CN106813968B - A kind of preparation method of hairtail sagitta cross section - Google Patents
A kind of preparation method of hairtail sagitta cross section Download PDFInfo
- Publication number
- CN106813968B CN106813968B CN201710072041.5A CN201710072041A CN106813968B CN 106813968 B CN106813968 B CN 106813968B CN 201710072041 A CN201710072041 A CN 201710072041A CN 106813968 B CN106813968 B CN 106813968B
- Authority
- CN
- China
- Prior art keywords
- otolith
- polishing
- hot melt
- melt adhesive
- hairtail
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/32—Polishing; Etching
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Dental Prosthetics (AREA)
Abstract
A kind of preparation method of hairtail sagitta cross section, the invention belongs to experimental technique fields, it includes the following steps：1) label otolith center；2) otolith is clamped on thin head self-locking forceps；3) otolith is embedded with hot melt adhesive；4) polishing otolith is to mark line；5) buffed surface of otolith after polishing is close to and is bonded on glass slide；6) it polishes since otolith front end, is polishing to otolith center, stopping when annual ring occurs, polishing plane is always perpendicular to the cephalocaudal axis of otolith.The present invention embeds otolith with hot melt adhesive, plays resin embedding and fixes and while protective effect, can be cooled to the degree that can be polished only several minutes；While saving resin embedding material, improve work efficiency.
Description
Technical field
The invention belongs to experimental technique fields, more particularly to a kind of preparation method of hairtail sagitta cross section.
Background technology
When carrying out hairtail Age Determination, sagitta (hairtail 3 to maximum 1 pair in otolith, hereinafter abbreviation otolith) is
Most commonly used material.Band fish otolith is shaped like rice grain, small and crisp fritter；The band fish otolith of 500 grams of weight, length and width
Only 5-6mm and 2.5mm or so, leisure opinion bodily form smaller person.Therefore in conventional method, when preparing otolith cross section, it is mostly used asphalt mixtures modified by epoxy resin
Fat is embedded, to carry out subsequent operation.It has main steps that：1) otolith center 2 is marked) carry out embedding 3 with resin) wait for
Resin embedding block solidification after, carry out cutting 4 with otolith cutting machine) on otolith polishing and burnishing machine with varigrained sand paper to cutting after
Resin embedding block carry out grinding and buffing, to obtain required otolith cross section.
Since the hardening time of resin embedding block is usually more than ten to twenties hours, required time is longer, therefore tight
Important place affects working efficiency.
Invention content
The technical problem to be solved in the present invention is to provide a kind of preparation method of hairtail sagitta cross section, the method
Embedding speed is fast, can be obtained band fish otolith cross section without cutting.
The present invention is achieved by the following technical solution：
A kind of preparation method of hairtail sagitta cross section, includes the following steps：
1) label otolith center；
2) otolith is clamped on thin head self-locking forceps；
3) otolith is embedded with hot melt adhesive；
Further, the specific method of the embedding is heating hot melt adhesive to 180 degrees Celsius；Otolith is immersed into hot melt adhesive liquid
In, otolith fore-end is reserved, hot melt adhesive is not adhered to, the other parts of otolith is allowed uniformly to be embedded by hot melt adhesive.
4) polishing otolith is to mark line；
Further, the polishing process is to polish since otolith rear end, and polishing plane is always perpendicular to the head of otolith
Tailing axle.
5) buffed surface of otolith after polishing is close to and is bonded on glass slide；
6) polish since otolith front end, be polishing to otolith center, stopping when annual ring occurs, polishing plane always perpendicular to
The cephalocaudal axis of otolith.
The advantageous effect of the present invention compared with prior art
The present invention does not use resin embedding otolith, uses hot melt adhesive instead and is embedded to otolith, plays resin embedding and fixes
While with protective effect, because hot melt adhesive cooling velocity is fast, the degree that can be polished can be cooled to only several minutes；So
Substantially eliminate the time needed for resin solidification.Meanwhile directly polished using polishing and burnishing machine otolith and after being cut with cutting machine again
In contrast, required time is suitable for the mode of polishing.Therefore while saving resin embedding material, work is preferably improved
Make efficiency.
Description of the drawings
Fig. 1 hairtail sagitta structural schematic diagrams：1- winged petioles, 2- auditory nerve ditches, the front ends 3-, 4- base leaves, 5- cephalocaudal axis, 6-
Ventral margin, the rear ends 7-, 8- otoliths center, 9- back edges, 10- dorsoventral axis；
Fig. 2 label otoliths center；
Otolith is clamped on thin head self-locking forceps by Fig. 3；
Fig. 4 embed otolith with hot melt adhesive；
Fig. 5 polish otolith for the first time；
Otolith is bonded on glass slide by Fig. 6；
Fig. 7 are polishing to otolith center, annual ring occurs.
Specific implementation mode
Embodiment
Technical scheme of the present invention is further explained below by embodiment combination attached drawing, but the protection of the present invention
Range is not limited in any form by embodiment.
1) it crosses otolith back edge peak with pencil and draws a line perpendicular to the wide about 0.6mm of its cephalocaudal axis with otolith center
(Fig. 2).
2) otolith after label is clamped on thin head self-locking forceps, tweezer point is placed on otolith in auditory nerve ditch, after otolith
It is outer (Fig. 3) that end (needing the part ground off) is placed in tweezer point.
3) utilize the hot melt adhesive (CrystalbondTM) in thermal station heating container to 180 degrees Celsius；Otolith is immersed and is heated
Glue reserves otolith fore-end, not adhere to hot melt adhesive (convenient for the otolith after polishing for the first time to be bonded on glass slide), allow
The other parts of otolith uniformly embed (Fig. 4) by hot melt adhesive.
4) otolith after embedding is placed on experimental bench；After hot melt adhesive cooling, hand-held tweezers are used on otolith polishing and burnishing machine
240 grit papers are polished；When polishing nearly to mark line, uses 400 and 600 grit papers instead successively and be polishing to mark line
(Fig. 5).
5) glass slide is placed in thermal station, temperature is adjusted to 160 degree or so.One hand-held self-locking forceps, by the otolith after polishing
It is placed on glass slide, buffed surface is close to glass slide；One holds thin head tweezers, after the hotmelt melts of otolith to be wrapped, open from
Tweezers are locked, the non-viscose glue part in otolith base leaf front end is clamped with thin head tweezers, is placed on glass slide (Fig. 6).
6) glass slide is removed from thermal station, after hot melt adhesive cooling, sets glass slide on glass slide fixture.Successively with 240,
The sand paper of 400 and 600 granularities is polished from otolith front end, is during which inspected with anatomical lens, be milled at otolith center, annual ring it is clear
Stop (Fig. 7) when appearance.
Claims (2)
1. a kind of preparation method of hairtail sagitta cross section, it is characterised in that it includes the following steps：
1) label otolith center；
2) otolith is clamped on thin head self-locking forceps；
3) otolith is embedded with hot melt adhesive；
4) polishing otolith is to mark line；
5) buffed surface of otolith after polishing is close to and is bonded on glass slide；
6) it polishes since otolith front end, is polishing to otolith center, stopping when annual ring occurs, polishing plane is always perpendicular to otolith
Cephalocaudal axis；The step 3）The specific method of embedding is heating hot melt adhesive to 180 degrees Celsius；Otolith is immersed into hot melt adhesive liquid
In, otolith fore-end is reserved, hot melt adhesive is not adhered to, the other parts of otolith is allowed uniformly to be embedded by hot melt adhesive.
2. a kind of preparation method of hairtail sagitta cross section according to claim 1, it is characterised in that the step 4）
Polishing process be polish since otolith rear end, polishing plane always perpendicular to the cephalocaudal axis of otolith.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710072041.5A CN106813968B (en) | 2017-02-09 | 2017-02-09 | A kind of preparation method of hairtail sagitta cross section |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710072041.5A CN106813968B (en) | 2017-02-09 | 2017-02-09 | A kind of preparation method of hairtail sagitta cross section |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106813968A CN106813968A (en) | 2017-06-09 |
| CN106813968B true CN106813968B (en) | 2018-10-12 |
Family
ID=59112577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710072041.5A Expired - Fee Related CN106813968B (en) | 2017-02-09 | 2017-02-09 | A kind of preparation method of hairtail sagitta cross section |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106813968B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU935742A1 (en) * | 1980-06-23 | 1982-06-15 | Московский областной научно-исследовательский институт акушерства и гинекологии | Method of localizing inflammatory process in placenta |
| CN101539495A (en) * | 2009-03-04 | 2009-09-23 | 上海海洋大学 | Preparation of mackerel otolith cross-sectional slices and method for determining age thereof |
| CN101548653A (en) * | 2009-05-12 | 2009-10-07 | 中国科学院水生生物研究所 | Method for researching age and migration life history of coilia by eardust |
| CN103231285A (en) * | 2013-04-26 | 2013-08-07 | 中国科学院水生生物研究所 | Manufacturing method and applications of otolith cross section grinding sheet of mandarin fish |
| CN103518655A (en) * | 2013-10-14 | 2014-01-22 | 中国水产科学研究院长江水产研究所 | Method for manufacturing section of xenophysogobio boulengeri otolith |
| CN104374615A (en) * | 2014-09-16 | 2015-02-25 | 中国水产科学研究院黑龙江水产研究所 | Schizothoracinae otolith slice production method |
| CN105136541A (en) * | 2015-09-21 | 2015-12-09 | 中国水产科学研究院淡水渔业研究中心 | Pretreatment method for micro-chemical analysis of otoliths of Coilia nasus |
-
2017
- 2017-02-09 CN CN201710072041.5A patent/CN106813968B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU935742A1 (en) * | 1980-06-23 | 1982-06-15 | Московский областной научно-исследовательский институт акушерства и гинекологии | Method of localizing inflammatory process in placenta |
| CN101539495A (en) * | 2009-03-04 | 2009-09-23 | 上海海洋大学 | Preparation of mackerel otolith cross-sectional slices and method for determining age thereof |
| CN101548653A (en) * | 2009-05-12 | 2009-10-07 | 中国科学院水生生物研究所 | Method for researching age and migration life history of coilia by eardust |
| CN103231285A (en) * | 2013-04-26 | 2013-08-07 | 中国科学院水生生物研究所 | Manufacturing method and applications of otolith cross section grinding sheet of mandarin fish |
| CN103518655A (en) * | 2013-10-14 | 2014-01-22 | 中国水产科学研究院长江水产研究所 | Method for manufacturing section of xenophysogobio boulengeri otolith |
| CN104374615A (en) * | 2014-09-16 | 2015-02-25 | 中国水产科学研究院黑龙江水产研究所 | Schizothoracinae otolith slice production method |
| CN105136541A (en) * | 2015-09-21 | 2015-12-09 | 中国水产科学研究院淡水渔业研究中心 | Pretreatment method for micro-chemical analysis of otoliths of Coilia nasus |
Non-Patent Citations (2)
| Title |
|---|
| 人工饲养和野生草鱼幼鱼耳石微结构的比较研究;宋昭彬 等;《水生生物学报》;20030131;第27卷(第1期);第7-12页 * |
| 广州从化拟细鲫基础生物学的研究;罗燕平;《华南师范大学2010学位年度硕士学位论文》;20101231;正文第16页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106813968A (en) | 2017-06-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US3959935A (en) | Abrasive pad for grinding lenses | |
| CN103712841A (en) | Production method for rock salt fluid inclusion flakes | |
| ATE516918T1 (en) | METHOD FOR GRINDING SHAFTS | |
| CN105458946B (en) | A kind of polishing disk for being used to polish curved-surface materials | |
| CN106813968B (en) | A kind of preparation method of hairtail sagitta cross section | |
| JP2017507796A (en) | Electric sharpener for ceramic and metal blades | |
| CN102172882A (en) | Method for grinding and preserving cephalopod otolith slices | |
| TW200626287A (en) | Method of sharpening cutting edges | |
| WO2012024267A3 (en) | Methods of grinding workpieces comprising superabrasive materials | |
| JP2005521565A5 (en) | ||
| CN102702982A (en) | Full grain leather polishing mending cream | |
| CN208496613U (en) | A kind of eyeglass grinding device | |
| CN103231285B (en) | A kind of preparation method of mandarin fish otolith cross section abrasive disc and application thereof | |
| US2024303A (en) | Glass polisher | |
| CN204639965U (en) | Heat radiating type frosted tape | |
| CN206084755U (en) | Bonding fixed glass's device | |
| CN102699838A (en) | Manufacture method of fleece abrasive cloth based on nonwoven fabrics | |
| JP2008260115A (en) | Blade whetting machine of hand plane | |
| GB578408A (en) | Improvements in or relating to abrasive-coated sheets or belts | |
| CN107971833A (en) | Bottom cover and preparation method thereof and mobile phone | |
| CN107336148A (en) | A kind of quick method for preparing abrasive particle pattern distribution mill | |
| CN209175539U (en) | A kind of adjustable polishing cloth conditioner discs in diamond grinding block position | |
| CN202763668U (en) | Fish otolith fixing clamp | |
| CN104136171A (en) | Method of dressing an abrasive wheel using a polycrystalline cvd synthetic diamond dresser and method of fabricating the same | |
| JPS6347049A (en) | Machining method for diamond member |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181012 Termination date: 20210209 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |