CN105738363B - A kind of method using Angle Overlap Model identification siphonopods age in days - Google Patents
A kind of method using Angle Overlap Model identification siphonopods age in days Download PDFInfo
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- CN105738363B CN105738363B CN201410767847.2A CN201410767847A CN105738363B CN 105738363 B CN105738363 B CN 105738363B CN 201410767847 A CN201410767847 A CN 201410767847A CN 105738363 B CN105738363 B CN 105738363B
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- 238000000034 method Methods 0.000 title claims abstract description 39
- 239000011347 resin Substances 0.000 claims abstract description 63
- 229920005989 resin Polymers 0.000 claims abstract description 63
- 238000000227 grinding Methods 0.000 claims abstract description 34
- 239000011521 glass Substances 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 229920000832 Cutin Polymers 0.000 claims description 15
- 210000002050 maxilla Anatomy 0.000 claims description 12
- 238000005498 polishing Methods 0.000 claims description 10
- 210000003625 skull Anatomy 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- 210000001847 jaw Anatomy 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000012535 impurity Substances 0.000 claims description 6
- 238000007517 polishing process Methods 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
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- -1 acryl Chemical group 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 238000002203 pretreatment Methods 0.000 claims description 3
- 235000013312 flour Nutrition 0.000 claims 2
- 238000003801 milling Methods 0.000 claims 2
- 238000002360 preparation method Methods 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 description 8
- PNEYBMLMFCGWSK-UHFFFAOYSA-N Alumina Chemical compound [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 6
- HBMJWWWQQXIZIP-UHFFFAOYSA-N silicon carbide Chemical compound [Si+]#[C-] HBMJWWWQQXIZIP-UHFFFAOYSA-N 0.000 description 6
- 229910010271 silicon carbide Inorganic materials 0.000 description 6
- 229920000297 Rayon Polymers 0.000 description 5
- 239000003292 glue Substances 0.000 description 5
- 241000238366 Cephalopoda Species 0.000 description 4
- 241000356847 Otolithes Species 0.000 description 4
- 210000003323 beak Anatomy 0.000 description 4
- 210000003128 head Anatomy 0.000 description 4
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- 239000004033 plastic Substances 0.000 description 4
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- NOQGZXFMHARMLW-UHFFFAOYSA-N Daminozide Chemical group CN(C)NC(=O)CCC(O)=O NOQGZXFMHARMLW-UHFFFAOYSA-N 0.000 description 2
- 102000004895 Lipoproteins Human genes 0.000 description 2
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- 230000000694 effects Effects 0.000 description 2
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- 210000003097 mucus Anatomy 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
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- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
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Abstract
The present invention provides a kind of method using Angle Overlap Model identification siphonopods age in days, the method that grinding including Angle Overlap Model rostral longitudinal section and growth line count mainly comprises the following steps extraction, cleaning, impregnates, cuts out, placing, resin embedding, cut, be fixed on glass slide, polish, polish, picture making and growing the processes such as line counting；It polishes the longitudinal section of Angle Overlap Model rostral, i.e., core face is ground to by the left and right sides to the midline respectively；The section made is taken pictures with CCD, to ensure that growth line can be read at any time from now on；Growth line remains that vertical direction counts by rostral rear end forward end along with growth line, ensures the accuracy counted；Solving the problem of Angle Overlap Model rostral, easy grinding grinding is not frangible and growth line is not easy to observe and count.
Description
Technical field
The invention belongs to Fisheries Science fields, relate generally to a kind of method using Angle Overlap Model identification siphonopods age in days.
Background technology
Siphonopods is under the jurisdiction of mollusk, generally annual, has the characteristics that it is important ocean warp that it is i.e. dead, which to have produced ovum,
Ji species, is distributed widely in the marine sites such as each ocean in the world and the South Pole.
In siphonopods fishery biology and ecology, Age Determination is important research content.Cephalopodous Age Determination
Method can be divided into indirectly and directly two kinds.Due to characteristics such as siphonopods growth fast, egg-laying season length, population structure complexity, cause profit
Estimate that the age is not accurate enough indirectly with its trunk length or weight.In all direct methods, reflected using the growth line in sclerous tissues
The fixed cephalopodous age is considered as most efficient method.
In the past few decades, otolith is widely used in cephalopodous age in days identification, however compared with otolith, as cephalopodous
The Angle Overlap Model individual bigger of one of sclerous tissues, easily extraction, grinding are convenient, and equally have the characteristics that information stable storing, but
It is rarely reported using Angle Overlap Model identification siphonopods age in days.
Angle Overlap Model is made of maxilla and lower jaw two parts, and upper and lower jaw structure is similar, by rostral, alar part, side wall, skull and
Ridge such as is dashed forward main 5 part compositions (attached drawing 1).As the sclerous tissueses such as Angle Overlap Model and otolith, inner casing, eye lens, exist apparent
Line structure is grown, the growth line on each portion surfaces such as Angle Overlap Model skull, ridge are dashed forward, side wall, alar part is apparent, and naked eyes are as it can be seen that but be not easy
Division identification, and the growth line rule in rostral, it is periodically apparent.In the prior art, the making of siphonopods Angle Overlap Model section
The rostral of Angle Overlap Model is usually directly cut off with scissors, so operation be easy to cause the braking effect growth line interpretation of rostral section；Its
The secondary grinding to Angle Overlap Model rostral is directly to grind, and on the one hand can cause the fracture of cutin in process of lapping, still further aspect meeting
The offset of ground flat is caused, it is relatively low to increase the damage to cutin, the success rate of microsection manufacture in process of lapping.With the exception of this,
Angle Overlap Model growth line counting is directly observed under the microscope in the prior art, growth line is highly inconvenient when counting；And cutin
Growth line counting direction is along rostral rear end to rostral top, starts to calculate the growth line of Angle Overlap Model back side and repetition measurement intersection
(dotted portion in figure), and some sub- day lines can be mistakenly considered a day line by this counting side, generate large error.
The content of the invention
It is an object of the invention to provide a kind of method using Angle Overlap Model identification siphonopods age in days, this method utilizes individual
Bigger is easily extracted, grinds convenient Angle Overlap Model to identify siphonopods age in days, includes the making step of siphonopods Angle Overlap Model section
Observation and counting step with siphonopods Angle Overlap Model growth line.
Second purpose of the invention also resides in the production method for providing a kind of siphonopods Angle Overlap Model section.
3rd purpose of the invention also resides in the observation for providing a kind of siphonopods Angle Overlap Model growth line and method of counting.
Technical scheme is as follows：
A kind of method using Angle Overlap Model identification siphonopods age in days, it is characterised in that：Include the following steps：
(1) cut：The maxilla of siphonopods Angle Overlap Model is taken out, cutting maxilla is split into two halves, and chooses half of Angle Overlap Model of embedding, will
Angle Overlap Model rostral is cut；
(2) place：The Angle Overlap Model rostral cut in step (1) is positioned in the groove of mold；
(3) resin embedding：By 1:1~1:1.5 volume ratio cold buries tree by what acryl powder and liquid curing-agent were configured to
Lipoprotein solution is poured into mold, and solution is made to cover mold, and the Angle Overlap Model rostral described in embedding step (2) is placed the room temperature place of being protected from light and treated
It is hardened；
(4) cut：The resin after hardening will be embedded centered on Angle Overlap Model rostral cut surface, cut into resin flake, and
Make abradant surface parallel to Angle Overlap Model rostral cut surface；
(5) it is fixed：The resin flake is fixed on along the direction parallel to Angle Overlap Model rostral cut surface on glass slide；
(6) polish：The resin flake being fixed on glass slide is polished, is first roughly ground to Angle Overlap Model rostral surface, then fine grinding
To close at Angle Overlap Model rostral center；
(7) polish：By the resin flake polishing grinding face through polishing, oil and impurity are removed, completes the resin flake
Grinding on one side；
(8) glass slide for being fixed with polished resin flake is impregnated in the hot water until resin softening, removes resin
After dry, resin flake turn-over is fixed on glass slide for thin slice, above-mentioned grinding and polishing process is repeated, until Angle Overlap Model beak
The growth line of portion's median plane is high-visible, obtains the section of Angle Overlap Model rostral；
(9) line is grown to count：The direction vertical along growth line counts the number of the growth line at rostral back side and back side edge,
It is cephalopodous age in days to grow line number.
The liquid curing-agent of the present invention is that viscosity is 60mPas, is purchased in the limited public affairs of the vast and boundless instrument and equipment in Shenzhen three
Department.
The method using Angle Overlap Model identification siphonopods age in days according to the present invention, it is preferred that cut out it in step (1)
Preceding is to include the following steps to cutin volume progress pre-treatment step：
(1) extract：Taking-up Angle Overlap Model out of cephalopodous mouth ball；
(2) clean：Angle Overlap Model after taking-up is cleaned；
(3) impregnate：Clean Angle Overlap Model is impregnated with 75% alcohol to be preserved for use.
The method using Angle Overlap Model identification siphonopods age in days according to the present invention, it is preferred that in the step (1)
Be cut to the hand-held cutting machine with blade along Angle Overlap Model rostral top to skull trailing edge cutting into two, choose bag half
Angle Overlap Model, then Angle Overlap Model rostral is cut.
The method using Angle Overlap Model identification siphonopods age in days according to the present invention, it is preferred that in the step (9)
Growth line be counted as：
(1) picture making：Section will be made to be placed on glass slide, drip supernatant water, then covered with rubber head dropper；
Be connected with the microscope 100 of CCD × times under take pictures to the growth line of the entire rostral of Angle Overlap Model, 400 × times lower diagonal
It takes pictures on matter jaw rostral top；With image processing software, picture mosaic processing is carried out respectively to the picture clapped under two multiples；
(2) line is grown to count：First hung down always along growth line by rostral rear end forward end to the 100 × times lower image gathered
Straight direction counts the number of the growth line of rostral back side, until rostral back side edge；Then to edge 400 × times under adopt
The image of collection continues to count growth line number.
The method using Angle Overlap Model identification siphonopods age in days according to the present invention, it is preferred that described in step (2)
It is placed as down lying against the cutting of Angle Overlap Model rostral among the groove of mold.
The method using Angle Overlap Model identification siphonopods age in days according to the present invention, it is preferred that described in step (4)
The thickness of resin flake is 2-3mm.
The method using Angle Overlap Model identification siphonopods age in days according to the present invention, it is preferred that described in step (8)
The glass slide of polished resin flake impregnates 20~30 minutes in the hot water.
The present invention also provides a kind of production methods of siphonopods Angle Overlap Model rostral section, which is characterized in that including walking as follows
Suddenly：
(1) cut：The maxilla of siphonopods Angle Overlap Model is taken out, cutting maxilla is split into two halves, and half of Angle Overlap Model of bag is chosen, by angle
Matter jaw rostral is cut；
(2) place：The Angle Overlap Model rostral section cut in step (1) is positioned in the groove of mold downwards；
(3) resin embedding：By 1:1~1:1.5 volume ratio cold buries tree by what acryl powder and liquid curing-agent were configured to
Lipoprotein solution is poured into mold, and solution is made to cover mold, and the Angle Overlap Model rostral described in embedding step (2) is placed the room temperature place of being protected from light and treated
It is hardened；
(4) cut：The resin after hardening will be embedded centered on Angle Overlap Model rostral cut surface, cut into resin flake, and
Make abradant surface parallel to Angle Overlap Model rostral cut surface；
(5) it is fixed：The resin flake is fixed on along the direction parallel to Angle Overlap Model rostral cut surface on glass slide；
(6) polish：The resin flake being fixed on glass slide is polished, is first roughly ground to Angle Overlap Model rostral surface, then fine grinding
To close at Angle Overlap Model rostral center；
(7) polish：By the resin flake polishing grinding face through polishing, oil and impurity are removed, completes the resin flake
Grinding on one side；
(8) glass slide for being fixed with polished resin flake is impregnated in the hot water, removes resin flake, after dry,
Resin flake turn-over is fixed on glass slide, above-mentioned grinding and polishing process is repeated, until the life of Angle Overlap Model rostral median plane
Long line is high-visible, obtains the section of Angle Overlap Model rostral.
The production method that the siphonopods Angle Overlap Model rostral section is provided according to the present invention, it is preferred that cut in step (1)
It is to include the following steps to carry out pre-treatment step to cutin volume before sanction：
(1) extract：Taking-up Angle Overlap Model out of cephalopodous mouth ball；
(2) clean：Angle Overlap Model after taking-up is cleaned；
(3) impregnate：Clean Angle Overlap Model is impregnated with 75% alcohol to be preserved for use.
The production method that the siphonopods Angle Overlap Model rostral section is provided according to the present invention, it is preferred that in the step
(1) it is cut to be cut into two along Angle Overlap Model rostral top to skull trailing edge with the hand-held cutting machine with blade in, chooses
Half of Angle Overlap Model is embedded, then Angle Overlap Model rostral is cut.
Observation and method of counting the present invention also provides a kind of siphonopods Angle Overlap Model growth line, which is characterized in that including such as
Lower step：
(1) picture making：Section will be made to be placed on glass slide, drip supernatant water, then covered with rubber head dropper；
Be connected with the microscope 100 of CCD × times under take pictures to the growth line of the entire rostral of Angle Overlap Model, 400 × times lower diagonal
It takes pictures on matter jaw rostral top；With image processing software, picture mosaic processing is carried out respectively to the picture clapped under two multiples；
(2) line is grown to count：First hung down always along growth line by rostral rear end forward end to the 100 × times lower image gathered
Straight direction counts the number of the growth line of rostral back side, until rostral back side edge；Then to edge 400 × times under adopt
The image of collection continues to count growth line number.
The production method that a kind of siphonopods Angle Overlap Model as second aspect of the present invention is cut into slices, is by following technical solution
Come what is realized：
(1) extract：The Angle Overlap Model in mouth ball is taken out with stainless steel flexibility tweezers；
(2) clean：Angle Overlap Model after taking-up first cleans the mucus on its surface and other organic matters with suds, then uses clear water
It cleans；
(3) impregnate：Clean Angle Overlap Model is put into nozzle bottle, is added in the alcohol that weight percent concentration is 75% and is impregnated
It preserves；
(4) cut：The Angle Overlap Model maxilla preserved is taken out, with the small hand-held cutting machine of the blade with 0.3mm along cutin
Jaw rostral top to skull trailing edge is cut into two (longitudinally cutting), half of Angle Overlap Model of embedding is chosen, with scissors by Angle Overlap Model beak
It cuts (attached drawing 3) in portion；
(5) place：The cutting of cutin rostral is made to lie against down among the groove of plastic mould (attached drawing 3)；
(6) resin embedding：By 1:1~1:Acryl powder and liquid curing-agent are configured to cold bury resin by 1.5 volume ratio
Deployed solution is poured into plastic mould by solution, and solution needs to cover mold, pour into it is cold bury resin embedding, and place room temperature
Treat its hardening in the place of being protected from light；
(7) cut：The resin mass after hardening will be wrapped centered on Angle Overlap Model rostral cut surface, cut into the thin slice of 2-3mm,
And make abradant surface parallel to Angle Overlap Model rostral cut surface；
(8) it is fixed：The resin mass of well cutting is glued along the direction parallel to Angle Overlap Model rostral cut surface with the fast viscose glue of strength
On glass slide fixed (attached drawing 3)；
(9) polish：In process of lapping, first roughly ground with 240 silicon carbide papers to Angle Overlap Model rostral surface, then with 600,1200,
2000 silicon carbide paper fine grindings, and observe its degree of grinding under an optical microscope at any time, until (attached close at Angle Overlap Model rostral center
Fig. 3)；
(10) polish：Water surface caused by flannelette polishing grinding face, removing silicon carbide paper is added with 0.05 μm of aluminium oxide agent
Cut and extra impurity so complete the grinding of one side；
(11) glass slide is impregnated in the hot water 10~30 minutes until resin softening, removes resin mass.After determining drying,
It is sticked to by resin mass turn-over, then with the fast viscose glue of strength on glass slide.Above-mentioned grinding and polishing process are repeated, until Angle Overlap Model rostral
The growth line of median plane is high-visible.
A kind of observation of siphonopods Angle Overlap Model growth line as third aspect present invention and method of counting, are by as follows
Technical solution is realized：
(1) picture making：Section will be made to be placed on glass slide, (increased and observed with the upper drop clear water of rubber head dropper drop
Clarity), then covered；In the microscope 100 × lower pair again for the Olympus for being connected with CCD (ccd image sensor)
The growth line of the entire rostral of Angle Overlap Model is taken pictures, 400 × times under take pictures to Angle Overlap Model rostral top；With
7.0 image processing softwares of Photoshop carry out picture mosaic processing respectively to the picture clapped under two multiples；
(2) line is grown to count：First hung down always along growth line by rostral rear end forward end to the 100 × times lower image gathered
(spacing between vertical direction growth line is maximum, and growth line is most clear for the number of the growth line of straight direction counting rostral back side
It is clear), until rostral back side edge；Then the image of edge 400 × times lower acquisition is continued to count growth line number (edge
Place's growth line spacing is too narrow, 100 × times under be not easy to distinguish) (attached drawing 4).
Invention applies usual microscope 40 ×, 100 × and 400 × times, 40 × times too small improper, from our reality
From the point of view of testing, 100 × and 400 × are most suitable again.
Advantageous effects：
Method provided by the invention using Angle Overlap Model identification siphonopods age in days, Angle Overlap Model individual bigger are easily extracted, ground
Mill is convenient, and information stable storing, the application identifies siphonopods age in days using Angle Overlap Model, and qualification result is more accurate.This hair
The fracture of rostral section is avoided using the rostral of minitype cutting machine cutting Angle Overlap Model in bright method, growth line is further avoided to miss
It reads；The grinding of Angle Overlap Model rostral buries resin embedding Angle Overlap Model rostral using cold in the method for the present invention, then cutting and grinding again, avoids
Fracture and the offset of ground flat in Angle Overlap Model rostral process of lapping add the success rate of microsection manufacture.The method of the present invention
It is counted in growth line, the convenience of growth line counting is greatly facilitated using camera system, overcomes and by mistake will in the prior art
Sub- day line is judged as the shortcomings that day line；The section made is taken pictures with CCD, to ensure that growth line can be read at any time from now on.
The present invention solves Angle Overlap Model rostral and is not easy directly grinding (although Angle Overlap Model is larger with respect to otolith, if not
If embedding, still seem too small, therefore grinding contact surface added after embedding), grinding is frangible and growth line is not easy to observe
The problem of with counting, provide a kind of method that can be quick and precisely ground to Angle Overlap Model rostral core, ensure higher film-making into
Power, while accurately interpretation growth line number.
Description of the drawings
Fig. 1 a are siphonopods Angle Overlap Model maxilla morphological parameters schematic diagram；
Fig. 1 b are siphonopods Angle Overlap Model lower jaw morphological parameters schematic diagram
Fig. 2 is siphonopods Angle Overlap Model rostral Longitudinal cross section schematic；
Fig. 3 is siphonopods Angle Overlap Model rostral microsection manufacture schematic diagram；
Fig. 4 cuts into slices for squid Angle Overlap Model, and (squid sample is collected in northwest Pacific, cutin on May 21st, 2010 to split figure
Jaw identification age in days 215 days；Blue arrow represents the direction that growth line counts, and yellow dot represents each growth line)
In figure：
1- beaks, 2- alar parts, 3- skulls, 4- ridges are dashed forward, 5- side walls, 6- beaks, 7- alar parts, 8- skulls, and 9- ridges are dashed forward, 10- side walls.
Specific embodiment
For a better understanding of the present invention, with reference to the embodiment content that the present invention is furture elucidated, but the present invention
Content is not limited solely to the following examples.
Embodiment：Utilize the method for Angle Overlap Model identification siphonopods age in days：(Angle Overlap Model age in days, squid are identified by taking squid as an example
Sample is collected in northwest Pacific on May 21st, 2010)
1st, extract：The Angle Overlap Model in mouth ball is carefully taken out with stainless steel flexibility tweezers, pays attention to beating easily, avoids damage to cutin
Jaw rostral；
2nd, clean：Angle Overlap Model after taking-up first cleans the mucus on its surface and other organic matters with suds, then uses clear water
It cleans；
3rd, impregnate：Clean Angle Overlap Model is put into nozzle bottle, is added in the alcohol that weight percent concentration is 75% and is impregnated,
And it is placed at dry and preserves；
4th, cut：The Angle Overlap Model maxilla preserved is taken out, with the small hand-held cutting machine of the blade with 0.3mm along Angle Overlap Model
Rostral top (pays attention to skull trailing edge is longitudinally cutting into two：During cutting, cutting line is slightly inclined to the one side of center line, makes at least
Cutin after one cutting includes center line)；That half of the Angle Overlap Model for including center line is chosen, is cut Angle Overlap Model rostral with scissors
(attached drawing 2)；
5th, place：Make cutin rostral cut surface (in next process of lapping, ground flat always keeping parallelism therewith)
It lies against downward among the groove of plastic mould, pays attention to having to be laid flat, in case ground flat tilts (attached drawing 3)；
6th, resin embedding：First grinding tool is numbered, the number of nozzle bottle of the number with preserving Angle Overlap Model is consistent, by 1:1
~1:Acryl powder and curing agent are configured to solution by 1.5 volume ratio, deployed solution are poured into plastic mould, solution needs
Cover mold, pour into it is cold bury resin embedding, and place room temperature be protected from light place 24 it is small when, treat its harden (attached drawing 3)；
7th, cut：The resin mass after hardening will be wrapped centered on Angle Overlap Model rostral cut surface, cut into the thin slice of 2-3mm,
And make abradant surface parallel to Angle Overlap Model rostral cut surface；
8th, it is fixed：Melt the fast viscose glue of strength, pick suitable strength speed viscose glue with pin, the resin mass of well cutting is sticked to load
On slide fixed (attached drawing 3)；
9th, polish：Glass slide is pinned with hand, is positioned on grinder, is first roughly ground with 240 silicon carbide papers to Angle Overlap Model rostral
Surface, then with 600,1200,2000 silicon carbide paper fine grindings, and observe its degree of grinding under an optical microscope at any time, until close
At Angle Overlap Model rostral center；
10th, polish：Water is added on flannelette polishing grinding face, surface stroke caused by removing silicon carbide paper with 0.05 μm of aluminium oxide agent
Trace and extra impurity.So complete the process of lapping of one side；
11st, glass slide is impregnated in the hot water 10~30 minutes until resin softening, removes resin mass.After determining drying
(at least 30 minutes or more) are sticked to by resin mass turn-over, then with the fast viscose glue of strength on glass slide.Repeat above-mentioned grinding and polishing
Process, until the growth line of Angle Overlap Model rostral median plane is high-visible.
12nd, finally the Angle Overlap Model polished section is put into scale bag and preserved, and marked.
13rd, picture making：Section will be made to be placed on glass slide, (increased and observed with the upper drop clear water of rubber head dropper drop
Clarity), then covered；Be connected with the microscope 100 of the Olympus of CCD × times under to the entire rostral of Angle Overlap Model
Growth line take pictures, 400 × times under take pictures to Angle Overlap Model rostral top；With 7.0 image procossings of Photoshop
Software carries out picture mosaic processing respectively to the picture clapped under two multiples；
14th, line is grown to count：First hung down always along growth line by rostral rear end forward end to the 100 × times lower image gathered
Straight direction count the growth line of rostral back side number (spacing between vertical direction growth line is maximum, and growth line is most clear,
Will not be influenced by sub- day line), until rostral back side edge；Then the image of edge 400 × times lower acquisition is continued to count
Number growth line number (edge growth line spacing is too narrow, 100 × under be not easy to distinguish) (attached drawing 4).
Angle Overlap Model identification age in days as shown in Figure 4 215 days；Blue arrow represents the direction that growth line counts, yellow dot generation
Table each grows line.
The main application of basic principle of the invention, main feature and the present invention has been shown and described above.The industry
For technical staff it should be appreciated that the present invention is not limited to the above embodiments, what is described in the above embodiment and the description is only say
Bright the principle of the present invention, various changes and modifications of the present invention are possible without departing from the spirit and scope of the present invention, this
A little changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims
And its equivalent defines.
Claims (6)
- A kind of 1. method using Angle Overlap Model identification siphonopods age in days, it is characterised in that：Include the following steps：(1) cut：The maxilla of siphonopods Angle Overlap Model is taken out, cutting maxilla is split into two halves, and half of Angle Overlap Model of embedding is chosen, by cutin Jaw rostral is cut；(2) place：The Angle Overlap Model rostral cut in step (1) is positioned in the groove of mold；(3) resin embedding：By 1:1~1:1.5 volume ratio by acryl powder and liquid curing-agent be configured to cold to bury resin molten Liquid is poured into mold, and solution is made to cover mold, and the Angle Overlap Model rostral described in embedding step (2) places the room temperature place of being protected from light and treats that it is hard Change；(4) cut：The resin after hardening will be embedded centered on Angle Overlap Model rostral cut surface, resin flake is cut into, and make to grind Flour milling is parallel to Angle Overlap Model rostral cut surface；(5) it is fixed：The resin flake is fixed on along the direction parallel to Angle Overlap Model rostral cut surface on glass slide；(6) polish：The resin flake being fixed on glass slide is polished, is first roughly ground to Angle Overlap Model rostral surface, then is finely ground to and connects At nearly Angle Overlap Model rostral center；(7) polish：By the resin flake polishing grinding face through polishing, oil and impurity are removed, completes the resin flake one side Grinding；(8) glass slide for being fixed with polished resin flake is impregnated in the hot water up to resin softening, removes resin flake, After drying, resin flake turn-over is fixed on glass slide, above-mentioned grinding and polishing process are repeated, until in Angle Overlap Model rostral The growth line in heart face is high-visible, obtains the section of Angle Overlap Model rostral；(9) line is grown to count：The direction vertical along growth line counts the number of the growth line at rostral back side and back side edge, growth Line number is cephalopodous age in days；Growth line in the step (9) is counted as：(1) picture making：Section will be made to be placed on glass slide, drip supernatant water, then covered with rubber head dropper；Even Be connected to the microscope 100 of CCD × times under take pictures to the growth line of the entire rostral of Angle Overlap Model, 400 × times under to Angle Overlap Model It takes pictures on rostral top；With image processing software, picture mosaic processing is carried out respectively to the picture clapped under two multiples；(2) line is grown to count：First to 100 × image of times lower acquisition by rostral rear end forward end, it is vertical along growth line always Direction counts the number of the growth line of rostral back side, until rostral back side edge；Then to edge 400 × times lower acquisition Image continues to count growth line number.
- 2. the method for Angle Overlap Model identification siphonopods age in days is utilized according to claim 1, which is characterized in that in the step (1) before cutting out, cutin volume is pre-processed, pretreatment the step of be：(1) extract：Taking-up Angle Overlap Model out of cephalopodous mouth ball；(2) clean：Angle Overlap Model after taking-up is cleaned；(3) impregnate：Clean Angle Overlap Model is impregnated with 75% alcohol to be preserved for use.
- 3. the method for Angle Overlap Model identification siphonopods age in days is utilized according to claim 1, which is characterized in that in the step (1) it is cut to be cut into two along Angle Overlap Model rostral top to skull trailing edge with the hand-held cutting machine with blade in, chooses Half of Angle Overlap Model is embedded, then Angle Overlap Model rostral is cut.
- 4. the method for Angle Overlap Model identification siphonopods age in days is utilized according to claim 1, which is characterized in that in step (2) It is described to be placed as down lying against the cutting of Angle Overlap Model rostral among the groove of mold.
- 5. the method for Angle Overlap Model identification siphonopods age in days is utilized according to claim 1, which is characterized in that in step (4) The thickness of the resin flake is 2-3mm.
- 6. observation and the method for counting of a kind of siphonopods Angle Overlap Model growth line, which is characterized in that include the following steps：(1) picture making：Section will be made to be placed on glass slide, drip supernatant water, then covered with rubber head dropper；Even Be connected to the microscope 100 of CCD × times under take pictures to the growth line of the entire rostral of Angle Overlap Model, 400 × times under to Angle Overlap Model It takes pictures on rostral top；With image processing software, picture mosaic processing is carried out respectively to the picture clapped under two multiples；(2) line is grown to count：First to 100 × image of times lower acquisition by rostral rear end forward end, it is vertical along growth line always Direction counts the number of the growth line of rostral back side, until rostral back side edge；Then to edge 400 × times lower acquisition Image continues to count growth line number；The preparation method of the section includes the following steps：(1) cut：The maxilla of siphonopods Angle Overlap Model is taken out, cutting maxilla is split into two halves, and half of Angle Overlap Model of embedding is chosen, by cutin Jaw rostral is cut；(2) place：The Angle Overlap Model rostral section cut in step (1) is positioned in the groove of mold downwards；(3) resin embedding：By 1:1~1:1.5 volume ratio by acryl powder and liquid curing-agent be configured to cold to bury resin molten Liquid is poured into mold, and solution is made to cover mold, and the Angle Overlap Model rostral described in embedding step (2) places the room temperature place of being protected from light and treats that it is hard Change；(4) cut：The resin after hardening will be embedded centered on Angle Overlap Model rostral cut surface, resin flake is cut into, and make to grind Flour milling is parallel to Angle Overlap Model rostral cut surface；(5) it is fixed：The resin flake is fixed on along the direction parallel to Angle Overlap Model rostral cut surface on glass slide；(6) polish：The resin flake being fixed on glass slide is polished, is first roughly ground to Angle Overlap Model rostral surface, then is finely ground to and connects At nearly Angle Overlap Model rostral center；(7) polish：By the resin flake polishing grinding face through polishing, oil and impurity are removed, completes the resin flake one side Grinding；(8) glass slide for being fixed with polished resin flake is impregnated in the hot water, removes resin flake, after dry, will set Fat thin slice turn-over, is fixed on glass slide, above-mentioned grinding and polishing process is repeated, until the growth line of Angle Overlap Model rostral median plane It is high-visible, obtain the section of Angle Overlap Model rostral；It is to include the following steps to carry out pre-treatment step to cutin volume before step (1) is cut out：(1) extract：Taking-up Angle Overlap Model out of cephalopodous mouth ball；(2) clean：Angle Overlap Model after taking-up is cleaned；(3) impregnate：Clean Angle Overlap Model is impregnated with 75% alcohol to be preserved for use；It is cut to be cut along Angle Overlap Model rostral top to skull trailing edge with the hand-held cutting machine with blade in the step (1) Two halves are cut into, half of Angle Overlap Model of embedding is chosen, then Angle Overlap Model rostral is cut.
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| CN106645150A (en) * | 2016-11-25 | 2017-05-10 | 上海海洋大学 | Method for determining ages of shrimps and crabs by using eyestalk |
| CN106970079B (en) * | 2017-02-27 | 2019-12-27 | 上海海洋大学 | Method for identifying day age of teleost by using inner shell |
| CN107279017B (en) * | 2017-07-12 | 2020-04-07 | 上海海洋大学 | Method for researching growth of squid individual by using inner shell forenail |
| CN109588348A (en) * | 2018-12-19 | 2019-04-09 | 上海海洋大学 | A method of measuring and calculating South China Sea Symeplectoteuthis oualaniensis individual growth and maturity based on Angle Overlap Model form |
| CN109724979A (en) * | 2019-01-11 | 2019-05-07 | 上海海洋大学 | A kind of preparation of Shrimp waste optic stalk slice and its Age Determination method |
| CN111693527A (en) * | 2020-06-05 | 2020-09-22 | 上海海洋大学 | Method for identifying day age of cuttlefish by using cuttlefish bone |
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