CN106645150A - Method for determining ages of shrimps and crabs by using eyestalk - Google Patents
Method for determining ages of shrimps and crabs by using eyestalk Download PDFInfo
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- CN106645150A CN106645150A CN201611050283.6A CN201611050283A CN106645150A CN 106645150 A CN106645150 A CN 106645150A CN 201611050283 A CN201611050283 A CN 201611050283A CN 106645150 A CN106645150 A CN 106645150A
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- optic stalk
- optic
- stalk
- base portion
- resin
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/32—Polishing; Etching
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
- G01N2001/364—Embedding or analogous mounting of samples using resins, epoxy
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
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- Engineering & Computer Science (AREA)
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Abstract
The invention relates to a method for determining ages of shrimps and crabs by using an eyestalk. The method comprises an eyestalk cross-section grinding method and a growth striation observation and counting method. The method comprises the main steps of extraction, washing, storing, degreasing, embedding with resin, cutting, fixing, grinding, polishing, picture fabricating, growth striation counting and the like, wherein the eyestalk cross-section grinding is to respectively grinding the eyestalk into a sheet from a basic portion and tail end of the eyestalk to a middle portion of the eyestalk; a fabricated slice is photographed with a CCE so as to ensure that the growth striation can be read at any time in the future; the growth striation is counted from the inner side to the outer side of the eyestalk; and the problems that the eyestalk is uneasy to grind and easy to drop when in grinding, and the growth striation is uneasy to observe and count can be solved.
Description
Technical field
The invention belongs to Fisheries Science field, more particularly to a kind of Shrimp waste optic stalk growth striae observation procedure.
Background technology
Shrimp waste is under the jurisdiction of Arthropoda, Crustachia, Decapoda, most species life-spans between 1～10 years old, it
It is important marine economy species, is distributed widely in the marine sites such as each ocean in the world and south, the arctic.Age Determination is used as Shrimp waste
Fishery biology and ecological key content, all the time by Chinese scholars extensive concern.However, Shrimp waste and fish
Other marine economic animals such as class, siphonopods are different, the method for lacking its age of Direct Identification.For Fish and siphonopods come
Say, their growth is recorded in the sclerous tissueses such as otolith, fin ray, Angle Overlap Model, and people can be by these sclerous tissueses' growth striae
Count to determine their age.However, the growth of Shrimp waste is different from Fish and siphonopods etc., grow when they are shellings,
When therefore shelling every time, original duricrust is substituted by new.Existing technology is by methods such as laboratory rearing, labelling recaptures
To obtain the age of Shrimp waste, this aspect is experimental period long, on the other hand cannot direct access field sample age.We
Method identifies the age of Shrimp waste by directly reading optic stalk growth striae number, provides one newly for the Direct Identification shrimp Eriocheir sinensiss age
Method.
The content of the invention
It is an object of the invention to provide a kind of method that utilization optic stalk identifies the Shrimp waste age, the method is steady using form
Determine, easily extract, grind convenient optic stalk to identify the age of Shrimp waste, including the making step and eye of Shrimp waste optic stalk section
The observation of handle growth striae and counting step.
Technical scheme is as follows：A kind of method that utilization optic stalk identifies the Shrimp waste age, it is characterised in that：Including
Following steps：
(1) cut out：Optic stalk is taken out, optic stalk base portion is cut flat, while the eyeball of optic stalk end is wiped out；
(2) embed：By 1:1～1:1.5 volume ratio by acryl powder and liquid curing-agent be configured to cold to bury resin molten
Liquid, pours in mould, makes solution full of hollow optic stalk, and covers mould, then places and its hardening is treated at room temperature lucifuge；
(3) cut：Cross-sectional cut resin thin slice of the resin after embedding is hardened along optic stalk；
(4) it is fixed：By the resin flake optic stalk end down, it is fixed on microscope slide；
(5) polish：The resin flake optic stalk base portion being fixed on microscope slide is polished, is first roughly ground to optic stalk base surface,
Optic stalk base portion is finely ground to again, optic stalk base portion is ground to completely by ground flat and is defined；
(6) polish：The resin flake polishing grinding face that Jing is polished, removes oil and impurity, completes the resin flake
The grinding of optic stalk base portion；
(7) polished resin flake by more than is placed on heating on warm table together with microscope slide until resin softening, removes
Resin flake, after being dried, resin flake turn-over is fixed on microscope slide, is then first roughly ground to base portion from optic stalk end, thin again
Mill is high-visible up to the growth striae of cross section, and last re-polishing obtains optic stalk section；
(8) growth striae observation：The number of growth striae is counted by optic stalk Inside To Outside.
According to the method that utilization optic stalk of the present invention identifies the Shrimp waste age, also can be by optic stalk base portion in step (4)
Sticked on microscope slide down, then from optic stalk end is to base portion polishing, polishing and implements subsequent step.
According to the method that utilization optic stalk of the present invention identifies the Shrimp waste age, it is preferred that before step (1) is cut out
Pretreatment is carried out to optic stalk, is comprised the steps：
(1) extract：With 1 pair of optic stalk of surgical scissorses clip Shrimp waste；
(2) clean：Will be optic stalk surface clean clean with clear water；
(3) remove organic matter：The optic stalk for cleaning up is put in clear water and is boiled 5 minutes, then with dissecting needle and tip
Muscle inside optic stalk and connective tissue are rejected by tweezers；
(4) preserve：The optic stalk that Jing steps (3) are processed is put in 75% ethanol and is preserved.
According to the method that utilization optic stalk of the present invention identifies the Shrimp waste age, it is preferred that in the step (8)
Growth striae counting step is：
(1) picture making：Section will be made to be placed on microscope slide, supernatant water be dripped with glue head dropper；It is being connected with CCD's
Microscope 400 × times under the growth striae of optic stalk is taken pictures, picture mosaic is carried out respectively to the picture clapped with image processing software
Process；
(2) growth striae is counted：Growth striae number is counted by optic stalk Inside To Outside to the image of 400 × times lower collection.
The present invention also provides a kind of manufacture method of Shrimp waste optic stalk section, it is characterised in that comprise the steps：
(1) cut out：Optic stalk is taken out, optic stalk base portion is cut flat, while the eyeball of optic stalk end is wiped out；
(2) embed：By 1:1～1:By acryl powder and liquid curing-agent, (viscosity is 60mPas to 1.5 volume ratio, buying
In the vast and boundless experimental instruments and equipment limited in Shenzhen three) be configured to it is cold bury resin solution, pour in mould, make solution full of hollow
Optic stalk, and mould is covered, then place and at room temperature lucifuge, treat its hardening；
(3) cut：Cross-sectional cut resin thin slice of the resin after embedding is hardened along optic stalk；
(4) it is fixed：The resin flake (optic stalk end is down) is fixed on microscope slide；
(5) polish：The resin flake being fixed on microscope slide (optic stalk base portion) is polished, is first roughly ground to optic stalk base portion table
Face, then be finely ground to optic stalk base portion (optic stalk base portion being ground to completely by ground flat to be defined)；
(6) polish：The resin flake polishing grinding face that Jing is polished, removes oil and impurity, completes the resin flake
The grinding of (optic stalk base portion)；
(7) polished resin flake by more than is placed on heating on warm table together with microscope slide until resin softening, removes
Resin flake, after being dried, resin flake turn-over is fixed on microscope slide, is then first roughly ground to base portion from optic stalk end, thin again
Mill is high-visible up to the growth striae of cross section, and last re-polishing obtains optic stalk section.
According to the manufacture method that the present invention provides the Shrimp waste optic stalk section, it is preferred that before step (1) is cut out
It is to comprise the steps that pre-treatment step is carried out to optic stalk：
(1) extract：With 1 pair of optic stalk of surgical scissorses clip Shrimp waste；
(2) clean：Will be optic stalk surface clearly clean with clear water；
(3) remove organic matter：Clear clean optic stalk is put in clear water and is boiled 5 minutes, then with dissecting needle and tip
Muscle inside optic stalk and connective tissue are rejected by tweezers etc..
According to the manufacture method that the present invention provides the Shrimp waste optic stalk section, it is preferred that in the step (1)
Optic stalk base portion is cut flat by cutting, while the ball portions of end must be wiped out；Tree is made when embedding described in the step (2)
Fat is full of hollow optic stalk；Also optic stalk base portion can be sticked on microscope slide down in the step (4), then by optic stalk end
To base portion polishing, polishing and enforcement subsequent step.
The present invention also provides a kind of observation and the method for counting of Shrimp waste optic stalk growth striae, it is characterised in that including as follows
Step：
(1) picture making：Section will be made to be placed on microscope slide, supernatant water be dripped with glue head dropper；It is being connected with CCD's
Microscope 400 × times under the growth striae of optic stalk is taken pictures, picture mosaic is carried out respectively to the picture clapped with image processing software
Process；
(2) growth striae is counted：Growth striae number is counted by optic stalk Inside To Outside to the image of 400 × times lower collection.
As a kind of manufacture method of the Shrimp waste section of second aspect present invention, it is by following technical solution realizing
's：
(1) extract：The optic stalk in eye socket is taken out with surgical scissorses；
(2) clean：Mucus and other Organic substances of the optic stalk after taking-up with its surface of clean water；
(3) remove organic matter：Clear clean optic stalk is put in clear water and is boiled 5 minutes, then with dissecting needle and tip
Muscle inside optic stalk and connective tissue are rejected by tweezers etc.；
(4) preserve：The optic stalk handled well, is put in centrifuge tube, add weight percent concentration be 75% it is alcohol-pickled
Preserve；
(5) cutting：The optic stalk for preserving is taken out, optic stalk base portion is cut flat with surgical scissorses, while by the eyeball of optic stalk end
Wipe out；
(6) embed：By 1:1～1:1.5 volume ratio by acryl powder and liquid curing-agent be configured to cold to bury resin molten
Liquid, pours in mould, makes solution full of hollow optic stalk, and covers mould, then places and its hardening is treated at room temperature lucifuge；
(7) cut：Cross-sectional cut of the resin after hardening along optic stalk will be embedded into 2-3mm resin flakes, and make grinding
Facet of the face parallel to optic stalk；
(8) it is fixed：The resin flake (optic stalk end is down) is bonded on microscope slide with the fast viscose glue of strength；
(9) polish：The resin flake being fixed on microscope slide (optic stalk base portion) is polished, in process of lapping, 240 water is first used
Dry sanding paper is roughly ground to optic stalk base surface, then with 600,1200,2000 silicon carbide paper fine grindings, and see under an optical microscope at any time
Its degree of grinding is examined, until optic stalk base portion (optic stalk base portion being ground to completely by ground flat to be defined)；
(10) polish：Added water in flannelette polishing grinding face with 0.05 μm of aluminium oxide agent, remove the surface that silicon carbide paper is caused
Cut and unnecessary impurity, so complete the grinding of optic stalk base portion；
(11) polished resin flake by more than is placed on heating on warm table together with microscope slide until resin softening, removes
Resin flake, after being dried, resin flake turn-over is fixed on microscope slide, then repeats above-mentioned to base portion from optic stalk end
Grinding and polishing process are until the growth striae of cross section is high-visible.
Observation and method of counting as a kind of Shrimp waste optic stalk growth striae of third aspect present invention, is by following skill
Art scheme is realizing：
(1) picture making：Section will be made to be placed on microscope slide, (increase observation with the upper drop clear water of glue head dropper drop
Definition)；The Olympus for being connected with CCD (ccd image sensor) microscope 400 × times under growth striae to optic stalk
Taken pictures；With 7.0 image processing softwares of Photoshop, the picture to being clapped carries out picture mosaic process respectively；
(2) growth striae is counted：Growth striae number is counted by optic stalk Inside To Outside to the image of 400 × times lower collection.
The method that the utilization optic stalk that the present invention is provided identifies the Shrimp waste age, optic stalk are easily extracted, grind convenient, information storage
Deposit stable, at the optic stalk identification Shrimp waste age, qualification result is more direct, more accurate.Operating scissorss are adopted before embedding in the inventive method
Knife cuts flat optic stalk base portion, is easy to find most suitable ground flat, further increases the accuracy of growth striae reading；The inventive method
The grinding of middle optic stalk buries resin embedding optic stalk using cold, then cutting and grinding again, it is to avoid the ground flat in optic stalk process of lapping
Skew, increased the success rate of microsection manufacture.The inventive method is greatly facilitated using camera system on growth striae is counted
The convenience that growth striae is counted；The section made is taken pictures with CCD, to ensure to read growth striae from now at any time.The present invention
Solve optic stalk and be difficult directly grinding (grinding contact surface is increased after embedding, ground flat deflection probability is reduced), grinding is easy
Come off and growth striae is difficult the problem observing and count, there is provided one kind can quick and precisely be ground to best fit plane, it is ensured that compared with
High film-making success rate, while accurately interpretation growth striae number.
Description of the drawings
Fig. 1 Shrimp waste optic stalks cut out schematic diagram.
Specific embodiment
Embodiment one：The method that the Shrimp waste age is identified using optic stalk, operating procedure are as follows：
1st, extract：The optic stalk in eye socket is taken out with surgical scissorses, note beating easily, it is to avoid damage optic stalk base portion；
2nd, clean：Mucus and other Organic substances of the optic stalk after taking-up with its surface of clean water；
3rd, remove organic matter：Clear clean optic stalk is put in clear water and is boiled 5 minutes, then with dissecting needle and probe forcepses
Muscle inside optic stalk and connective tissue are rejected by son etc.；
4th, preserve：The optic stalk handled well, is put in centrifuge tube, adds the alcohol-pickled guarantor that weight percent concentration is 75%
Deposit；
5th, cutting：The optic stalk (Figure 1A) for preserving is taken out, optic stalk base portion is cut flat into (Figure 1B) with surgical scissorses, while by optic stalk
The eyeball of end wipes out (Fig. 1 C)；
6th, embed：By 1:1～1:1.5 volume ratio by acryl powder and liquid curing-agent (viscosity is 60mPas, purchase in
The vast and boundless experimental instruments and equipment limited in Shenzhen three) be configured to it is cold bury resin solution, pour in mould, make solution be full of hollow eye
Handle, and mould is covered, then place and at room temperature lucifuge, treat its hardening；
7th, cut：Cross-sectional cut of the resin after hardening along optic stalk will be embedded into 2-3mm resin flakes, and make abradant surface
Parallel to the facet of optic stalk；
8th, it is fixed：The resin flake (optic stalk end is down) is bonded on microscope slide with the fast viscose glue of strength；
9th, polish：The resin flake being fixed on microscope slide (optic stalk base portion) is polished, in process of lapping, 240 water is first used
Dry sanding paper is roughly ground to optic stalk base surface, then with 600,1200,2000 silicon carbide paper fine grindings, and see under an optical microscope at any time
Its degree of grinding is examined, until optic stalk base portion (optic stalk base portion being ground to completely by ground flat to be defined)；
10th, polish：Added water in flannelette polishing grinding face with 0.05 μm of aluminium oxide agent, remove the surface stroke that silicon carbide paper is caused
Trace and unnecessary impurity, so complete the grinding of optic stalk base portion；
11st, polished resin flake by more than is placed on heating on warm table together with microscope slide until resin softening, removes
Resin flake, after being dried, resin flake turn-over is fixed on microscope slide, then repeats above-mentioned to base portion from optic stalk end
Grinding and polishing process are until the growth striae of cross section is high-visible；
12nd, the optic stalk polished section is put in paper bag and is preserved, and carry out labelling；
13rd, picture making：Section will be made to be placed on microscope slide, (increase observation with the upper drop clear water of glue head dropper drop
Definition)；The Olympus for being connected with CCD (ccd image sensor) microscope 400 × times under growth striae to optic stalk
Taken pictures；With 7.0 image processing softwares of Photoshop, the picture to being clapped carries out picture mosaic process respectively；
14th, growth striae is counted：Growth striae number is counted by optic stalk Inside To Outside to the image of 400 × times lower collection.
Embodiment two：A kind of manufacture method of Shrimp waste optic stalk section, operating procedure are as follows：
(1) cut out：Optic stalk is taken out, optic stalk base portion is cut flat, while the eyeball of optic stalk end is wiped out；
(2) embed：By 1:1～1:By acryl powder and liquid curing-agent, (viscosity is 60mPas to 1.5 volume ratio, buying
In the vast and boundless experimental instruments and equipment limited in Shenzhen three) be configured to it is cold bury resin solution, pour in mould, make solution full of hollow
Optic stalk, and mould is covered, then place and at room temperature lucifuge, treat its hardening；
(3) cut：Cross-sectional cut resin thin slice of the resin after embedding is hardened along optic stalk；
(4) it is fixed：The resin flake (optic stalk end is down) is fixed on microscope slide；
(5) polish：The resin flake being fixed on microscope slide (optic stalk base portion) is polished, is first roughly ground to optic stalk base portion table
Face, then be finely ground to optic stalk base portion (optic stalk base portion being ground to completely by ground flat to be defined)；
(6) polish：The resin flake polishing grinding face that Jing is polished, removes oil and impurity, completes the resin flake
The grinding of (optic stalk base portion)；
(7) polished resin flake by more than is placed on heating on warm table together with microscope slide until resin softening, removes
Resin flake, after being dried, resin flake turn-over is fixed on microscope slide, is then first roughly ground to base portion from optic stalk end, thin again
Mill is high-visible up to the growth striae of cross section, and last re-polishing obtains optic stalk section.
According to the manufacture method that the present invention provides the Shrimp waste optic stalk section, it is preferred that before step (1) is cut out
It is to comprise the steps that pre-treatment step is carried out to optic stalk：
(1) extract：With 1 pair of optic stalk of surgical scissorses clip Shrimp waste；
(2) clean：Will be optic stalk surface clearly clean with clear water；
(3) remove organic matter：Clear clean optic stalk is put in clear water and is boiled 5 minutes, then with dissecting needle and tip
Muscle inside optic stalk and connective tissue are rejected by tweezers etc..
According to the manufacture method that the present invention provides the Shrimp waste optic stalk section, it is preferred that in the step (1)
Optic stalk base portion is cut flat by cutting, while the ball portions of end must be wiped out；Tree is made when embedding described in the step (2)
Fat is full of hollow optic stalk；Also optic stalk base portion can be sticked on microscope slide down in the step (4), then by optic stalk end
To base portion polishing, polishing and enforcement subsequent step.
Embodiment three：A kind of observation of Shrimp waste optic stalk growth striae and method of counting, operating procedure are as follows：
(1) picture making：Section will be made to be placed on microscope slide, (increase observation with the upper drop clear water of glue head dropper drop
Definition)；The Olympus for being connected with CCD (ccd image sensor) microscope 400 × times under growth striae to optic stalk
Taken pictures；With 7.0 image processing softwares of Photoshop, the picture to being clapped carries out picture mosaic process respectively；
(2) growth striae is counted：Growth striae number is counted by optic stalk Inside To Outside to the image of 400 × times lower collection.
Claims (8)
1. a kind of method that utilization optic stalk identifies the Shrimp waste age, it is characterised in that：Comprise the steps：
(1) cut out：Optic stalk is taken out, optic stalk base portion is cut flat, while the eyeball of optic stalk end is wiped out；
(2) embed：By 1:1～1:1.5 volume ratio by acryl powder and liquid curing-agent be configured to it is cold bury resin solution,
Enter in mould, solution is made full of hollow optic stalk, and cover mould, then place and its hardening is treated at room temperature lucifuge；
(3) cut：Cross-sectional cut resin thin slice of the resin after embedding is hardened along optic stalk；
(4) it is fixed：The resin flake optic stalk end is fixed on microscope slide down；
(5) polish：The resin flake optic stalk base portion being fixed on microscope slide is polished, is first roughly ground to optic stalk base surface, then carefully
Optic stalk base portion is milled to, optic stalk base portion is ground to completely by ground flat and is defined；
(6) polish：The resin flake polishing grinding face that Jing is polished, removes oil and impurity, completes the resin flake optic stalk
The grinding of base portion；
(7) polished resin flake by more than is placed on heating on warm table together with microscope slide until resin softening, removes resin
Thin slice, be dried after, resin flake turn-over is fixed on microscope slide, then first roughly ground from optic stalk end to base portion, again fine grinding it is straight
Growth striae to cross section is high-visible, and last re-polishing obtains optic stalk section；
(8) growth striae observation：The number of growth striae is counted by optic stalk Inside To Outside.
2. the method that a kind of utilization optic stalk according to claim 1 identifies the Shrimp waste age, it is characterised in that in the step
Suddenly pretreatment is carried out to optic stalk before (1) cuts out, step is：
(1) extract：With 1 pair of optic stalk of surgical scissorses clip Shrimp waste；
(2) clean：Will be optic stalk surface clean clean with clear water；
(3) remove organic matter：The optic stalk for cleaning up is put in clear water and is boiled 5 minutes, then with dissecting needle and tip tweezers
Muscle inside optic stalk and connective tissue are rejected；
(4) preserve：The optic stalk of step (3) is put in 75% ethanol and is preserved.
3. the method that a kind of utilization optic stalk according to claim 1 identifies the Shrimp waste age, it is characterised in that in step (4)
Optic stalk base portion is sticked on microscope slide down, then from optic stalk end is to base portion polishing, polishing and implements subsequent step.
4. the method that a kind of utilization optic stalk according to claim 1 identifies the Shrimp waste age, it is characterised in that the step
(8) the growth striae counting step in is：
(1) picture making：Section will be made to be placed on microscope slide, supernatant water be dripped with glue head dropper；It is being connected with the micro- of CCD
Mirror 400 × times under the growth striae of optic stalk is taken pictures, picture mosaic process is carried out respectively to the picture clapped with image processing software；
(2) growth striae is counted：Growth striae number is counted by optic stalk Inside To Outside to the image of 400 × times lower collection.
5. the manufacture method that a kind of Shrimp waste optic stalk is cut into slices, it is characterised in that comprise the steps：
(1) cut out：Optic stalk is taken out, optic stalk base portion is cut flat, while the eyeball of optic stalk end is wiped out；
(2) embed：By 1:1～1:1.5 volume ratio by acryl powder and liquid curing-agent be configured to it is cold bury resin solution,
Enter in mould, solution is made full of hollow optic stalk, and cover mould, then place and its hardening is treated at room temperature lucifuge；
(3) cut：Cross-sectional cut resin thin slice of the resin after embedding is hardened along optic stalk；
(4) it is fixed：The resin flake optic stalk end is fixed on microscope slide down；
(5) polish：The resin flake optic stalk base portion being fixed on microscope slide is polished, is first roughly ground to optic stalk base surface, then carefully
Optic stalk base portion is milled to, optic stalk base portion is ground to completely by ground flat and is defined；
(6) polish：The resin flake polishing grinding face that Jing is polished, removes oil and impurity, completes the resin flake optic stalk
The grinding of base portion；
(7) polished resin flake by more than is placed on heating on warm table together with microscope slide until resin softening, removes resin
Thin slice, be dried after, resin flake turn-over is fixed on microscope slide, then first roughly ground from optic stalk end to base portion, again fine grinding it is straight
Growth striae to cross section is high-visible, and last re-polishing obtains optic stalk section.
6. the manufacture method that a kind of Shrimp waste optic stalk according to claim 5 is cut into slices, it is characterised in that cut in step (1)
It is to comprise the steps to carry out pre-treatment step before sanction to optic stalk：
(1) extract：With 1 pair of optic stalk of surgical scissorses clip Shrimp waste；
(2) clean：Will be optic stalk surface clearly clean with clear water；
(3) remove organic matter：Clear clean optic stalk is put in clear water and is boiled 5 minutes, then with dissecting needle and tip tweezers
Muscle inside optic stalk and connective tissue are rejected；
(4) preserve：By the optic stalk of step (3), and preserve in being put into 75% ethanol.
7. the manufacture method that a kind of Shrimp waste optic stalk according to claim 5 is cut into slices, it is characterised in that in the step (4)
Optic stalk base portion is sticked on microscope slide down, then from optic stalk end is to base portion polishing, polishing and implements subsequent step.
8. a kind of observation of Shrimp waste optic stalk growth striae and method of counting, it is characterised in that comprise the steps：
(1) picture making：Section will be made to be placed on microscope slide, supernatant water be dripped with glue head dropper；It is being connected with the micro- of CCD
Mirror 400 × times under the growth striae of optic stalk is taken pictures, picture mosaic process is carried out respectively to the picture clapped with image processing software；
(2) growth striae is counted：Growth striae number is counted by optic stalk Inside To Outside to the image of 400 × times lower collection.
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Cited By (5)
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|---|---|---|---|---|
| CN107328778A (en) * | 2017-07-12 | 2017-11-07 | 上海海洋大学 | A kind of method that utilization gastric mill identifies the Shrimp waste age |
| CN109724979A (en) * | 2019-01-11 | 2019-05-07 | 上海海洋大学 | A kind of preparation of Shrimp waste optic stalk slice and its Age Determination method |
| CN110243640A (en) * | 2018-03-08 | 2019-09-17 | 中国科学院海洋研究所 | The inner cavity of deep-sea foraminifer is ground fast preparation method |
| CN112729982A (en) * | 2020-12-21 | 2021-04-30 | 上海海洋大学 | Preparation method of material for identifying age of shark |
| CN114740003A (en) * | 2022-04-24 | 2022-07-12 | 烟台大学 | Method for rapidly identifying age of crustacean |
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| MICHAEL SHERIDAN 等: "INVESTIGATING THE FEASIBILITY OF USING GROWTH INCREMENTS FOR AGE DETERMINATION OF NORWAY LOBSTER (NEPHROPS NORVEGICUS) AND BROWN CRAB (CANCER PAGURUS）", 《JOURNAL OF CRUSTACEAN BIOLOGY》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107328778A (en) * | 2017-07-12 | 2017-11-07 | 上海海洋大学 | A kind of method that utilization gastric mill identifies the Shrimp waste age |
| CN110243640A (en) * | 2018-03-08 | 2019-09-17 | 中国科学院海洋研究所 | The inner cavity of deep-sea foraminifer is ground fast preparation method |
| CN109724979A (en) * | 2019-01-11 | 2019-05-07 | 上海海洋大学 | A kind of preparation of Shrimp waste optic stalk slice and its Age Determination method |
| CN112729982A (en) * | 2020-12-21 | 2021-04-30 | 上海海洋大学 | Preparation method of material for identifying age of shark |
| CN114740003A (en) * | 2022-04-24 | 2022-07-12 | 烟台大学 | Method for rapidly identifying age of crustacean |
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