CN107328778A - A kind of method that utilization gastric mill identifies the Shrimp waste age - Google Patents
A kind of method that utilization gastric mill identifies the Shrimp waste age Download PDFInfo
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- CN107328778A CN107328778A CN201710564807.1A CN201710564807A CN107328778A CN 107328778 A CN107328778 A CN 107328778A CN 201710564807 A CN201710564807 A CN 201710564807A CN 107328778 A CN107328778 A CN 107328778A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/32—Polishing; Etching
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
- G01N2001/364—Embedding or analogous mounting of samples using resins, epoxy
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
- G01N2001/366—Moulds; Demoulding
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Abstract
The invention discloses a kind of method that utilization gastric mill identifies the Shrimp waste age, this method include mark, choose, embedding, cutting, fixed, polishing, polishing, heating, the step such as growth line observation.The present invention provides a new solution for the Direct Identification shrimp crab age, with significant research and commercial value.
Description
Technical field
The invention belongs to Fisheries Science field, specifically, a kind of method that utilization gastric mill identifies the Shrimp waste age is disclosed, should
Method is related to the observation that Shrimp waste gastric mill grows line.
Background technology
Shrimp waste is under the jurisdiction of Arthropoda, Crustachia, Decapoda, most species life-spans between 1~10 years old, it
Be important marine economy species, be distributed widely in the marine sites such as each ocean in the world and south, the arctic.Age Determination is fisheries biology
The important content of research is learned, by the identification to the aquatile age, helps to grasp its history of life and estimates its population quantity,
Back ground Information is provided for fishery resources.
It is biological for most of wild water, typically its age can be directly obtained by the growth line in sclerous tissues
Fin ray, the vertebrae of information, such as fish, cephalopodous otolith, Angle Overlap Model and inner casing etc..It is most of to learn in the research of early stage
Person is using raising, mark is recaptured, the analysis of the long frequency of body, lipofuscin and the method such as radioisotope analysis are crustacean to identify
Age, but these methods come with some shortcomings.In addition, different from fish and siphonopods, shellfish is by shelling
Journey is grown, therefore can be recorded the calcified structure of age information and periodically disappeared, and to Direct Identification, its age brings necessarily
Difficulty.
One of a small number of sclerous tissueses that can still retain when gastric mill is Shrimp waste shelling, so far there are no for this area is ground using gastric mill
Study carefully the report at the age of Shrimp waste.The present invention identifies the age of Shrimp waste by directly reading gastric mill growth line number, is straight
The identification shrimp crab age is connect there is provided a new effective method.
The content of the invention
The technical problem to be solved in the present invention is that deficiency and difficulty for identifying the Shrimp waste age in the prior art are carried
A kind of method at effective Direct Identification shrimp crab age is supplied.
To solve the above problems, the invention provides a kind of method that utilization gastric mill identifies the Shrimp waste age, its feature exists
In：Comprise the following steps：
(1) mark：Yoke ossicula cardia is chosen, one article of line of cut is drawn at the 1st tooth and near-end 1/2 according to Fig. 1；
(2) embed：The yoke ossicula cardia for being decorated with line of cut is lain against in grinding tool, acryl powder is poured into and liquid curing-agent is mixed
The resin solution being configured to is closed, solution is covered whole mould, then places and treats that it is hardened at room temperature lucifuge；
(3) cut：The resin mass after hardening will be embedded centered on line of cut, by the unnecessary Partial Resection in both sides；
(4) it is fixed：By the resin flake side after cutting down, it is fixed on slide；
(5) polish：By the resin flake opposite side being fixed on slide polishing, successively with 120,600,1200,2500
Purpose silicon carbide paper, first roughly grinds and is finely ground to line of cut surface afterwards；
(6) polish：By the resin flake polishing grinding face through polishing, oil and impurity are removed, the resin flake is completed
Grinding simultaneously；
(7) resin flake polished by more than is placed on heating on warm table until resin softening, is removed together with slide
Resin flake, after drying, resin flake turn-over is fixed on slide, then repeats (5) and (6) step up to section
Grow line high-visible；
(8) growth line observation：Count the number of growth line in calcified layer.
Further, gastric mill is pre-processed before step (1) mark, comprised the following steps：
(1) extract：The carapace of Shrimp waste is opened, its whole stomach is taken out, stomach is cut off with surgical scissors and takes out gastric mill；
(2) clean：Gastric mill is cut off with surgical scissors and is deployed, it is with clear water that gastric mill surface clean is clean；
(3) connective tissue is removed：The connective tissue being connected with gastric mill bone is rejected with dissecting scissors and tip tweezers to reject；
(4) preserve：The gastric mill that will be handled through step (3) freezen protective at -20 DEG C.
Further, the growth line counting step in the step (8) is：
(1) picture making：Section will be made to be placed on slide, supernatant water is dripped with glue head dropper；It is being connected with CCD's
Microscope 100 × times under the growth line of gastric mill is taken pictures, picture mosaic is carried out respectively to the picture clapped with image processing software
Processing；
(2) growth line is counted：Number of lines counting is entered to the growth line of calcified layer to the image of 100 × times lower collection.
In some embodiments, chosen and ground according to Fig. 1 as material with wing ossicula cardia or middle ossicula cardia in step (1)
Flat surface grinding, then completes embedding, cutting, fixed, polishing, polishing and implements subsequent step.
Preferably, described grinding tool is plastic mould.
Preferably, the configuration volume ratio for stating acryl powder and liquid curing-agent is 1:1-1:1.5.
Definition：
" shrimp crab ", is another family of arthropod, belongs to Crustacea Decapoda.This kind of animals and human beingses class has very
Close relationship, some are main aquaculture or fished species, wherein being caught especially with shrimp, lobster and crab etc. in sea fishery
Obtain yield in thing quite big, particularly prawn, shrimp, swimming crab etc., nutritious, the output value is very high, and status is even more important.China
Shrimp crab species class it is very many, by substantial amounts of investigation, what is had now been found that there are about kind more than 1000, wherein shrimps kind more than 400,
Crab class kind more than 600.According to the teachings of the present invention, those skilled in the art can apply to the inventive method any Shrimp waste life
Thing, therefore the invention is not restricted to any specific Shrimp waste is biological.
" gastric mill (gastric mill) ", i.e., so-called chewing stomach, the cuticula of its inner face forms gastric teeth at certain position, dashed forward
For stomach intracavitary, the control based on muscle can grind food.This mastication texture on coat of the stomach is referred to as gastric mill.
" acryl powder ", is a kind of exploitation important thermoplastic earlier, with the preferable transparency, chemical stability
And weatherability, easy dyeing, easy processing, attractive appearance.
" curing agent " curing agent also known as curing agent, curing agent or set agent, are that a class is promoted or controls the thing of curing reaction
Matter, can be divided into liquid curing-agent and solid curing agent.Those skilled in the art can select suitable liquid according to actual conditions
Curing agent, the invention is not restricted to any specific liquid curing-agent.
Beneficial effects of the present invention：
1. solve the deficiency at the age of identification Shrimp waste in the prior art and difficulty.
2. identifying the age of Shrimp waste by directly reading gastric mill growth line number, provided for the Direct Identification shrimp crab age
One new effective method.
Brief description of the drawings
With reference to accompanying drawing and the specific embodiment of the invention, those skilled in the art will become more apparent that the present invention it is above-mentioned with
And other purposes, advantages and features.
Fig. 1 is that Shrimp waste gastric mill structure selects schematic diagram with ground flat, wherein, m：Middle ossicula cardia, z：Yoke ossicula cardia, p：
Wing ossicula cardia, mt：Middle ossicula cardia, zt：Yoke ossicula cardia, pt：Wing ossicula cardia.
A：Section is at proximally and distally 1/2；
B：Section is located at the 1st tooth and near-end 1/2；
C：At left and right 1/2.
Fig. 2 is Portunus trituberculatus Miers yoke orifice of the stomach bone micro-structure figure.
Embodiment
Raw materials used and equipment is that those skilled in the art know in embodiment, and be in the market can buy or
It is readily available or is made.
The method that embodiment 1. identifies the Portunus trituberculatus Miers age using gastric mill, operating procedure is as follows：
(1) extract：The carapace for opening Shrimp waste takes out its whole stomach, and stomach is cut off with surgical scissors and takes out gastric mill；
(2) clean：Gastric mill is cut off with surgical scissors and is deployed, it is with clear water that gastric mill surface clean is clean；
(3) connective tissue is removed：Rejected and gastric mill bone (1 middle ossicula cardia, 1 pair of yoke with dissecting scissors and tip tweezers
Ossicula cardia and 1 pair of wing ossicula cardia) connected connective tissue；
(4) preserve：The gastric mill that will be handled through step (3) freezen protective at -20 DEG C；
(5) mark：Choose 1 yoke ossicula cardia, according to Fig. 1 at the 1st tooth and near-end 1/2 with the one article of cutting of mark stroke
Line；
(6) embed：The yoke ossicula cardia for being decorated with line of cut is lain against in plastics grinding tool, poured into by 1:1-1:1.5 volume
The resin solution being configured to than the acryl powder and liquid curing-agent of mixing, makes solution cover whole mould, then places room temperature
Treat that it is hardened at lucifuge；
(7) cut：The resin mass after hardening will be embedded centered on line of cut, by the unnecessary Partial Resection in both sides；
(8) it is fixed：By the resin flake side after cutting down, it is fixed on slide；
(9) polish：By the resin flake opposite side being fixed on slide polishing, successively with 120,600,1200,2500
Purpose silicon carbide paper, first roughly grinds and is finely ground to line of cut surface afterwards；
(10) polish：By the resin flake polishing grinding face through polishing, oil and impurity are removed, the resin flake is completed
Grinding simultaneously；
(11) resin flake polished by more than is placed on heating on warm table until resin softening, is removed together with slide
Resin flake, after drying, resin flake turn-over is fixed on slide, then repeats (9) and (10) step up to section
Grow line high-visible；
(12) picture making：Section will be made to be placed on slide, supernatant water is dripped with glue head dropper；It is being connected with CCD
Microscope 100 × times under the growth line of gastric mill is taken pictures, the picture clapped is spelled respectively with image processing software
Figure processing；
(13) growth line is counted：Number of lines counting is entered to the growth line of calcified layer to the image of 100 × times lower collection.
By above-mentioned steps, the age of Direct Identification Portunus trituberculatus Miers is able to.
Described above is only the specific embodiment of the present invention, not does any formal limitation to the present invention, though
So the present invention is disclosed above with specific embodiment, but is not limited to the present invention, any to be familiar with this professional technology people
Member, in the range of technical solution of the present invention is not departed from, when the technology contents using the disclosure above make a little change or repair
The equivalent embodiment for equivalent variations is adornd, as long as being the content without departing from technical solution of the present invention, the technology according to the present invention is real
Any simple modification, equivalent variations and modification that confrontation above example is made, still fall within the scope of technical solution of the present invention
It is interior.
Claims (6)
1. a kind of method that utilization gastric mill identifies the Shrimp waste age, it is characterised in that：Comprise the following steps：
(1) mark：Yoke ossicula cardia is chosen, one article of line of cut is drawn at the 1st tooth and near-end 1/2；
(2) embed：The yoke ossicula cardia for being decorated with line of cut is lain against in grinding tool, acryl powder is poured into and liquid curing-agent mixing is matched somebody with somebody
The resin solution being set to, makes solution cover whole mould, then places and treats that it is hardened at room temperature lucifuge；
(3) cut：The resin mass after hardening will be embedded centered on line of cut, by the unnecessary Partial Resection in both sides；
(4) it is fixed：By the resin flake side after cutting down, it is fixed on slide；
(5) polish：By the resin flake opposite side being fixed on slide polishing, successively with 120,600,1200,2500 purposes
Silicon carbide paper, first roughly grinds and is finely ground to line of cut surface afterwards；
(6) polish：By the resin flake polishing grinding face through polishing, oil and impurity are removed, the resin flake one side is completed
Grinding；
(7) resin flake polished by more than is placed on heating on warm table until resin softening, removes resin together with slide
Thin slice, after drying, resin flake turn-over is fixed on slide, then repeats (5) and (6) step until the growth in section
Line is high-visible；
(8) growth line observation：Count the number of growth line in calcified layer.
2. according to the method described in claim 1, it is characterised in that gastric mill is pre-processed before step (1) mark, wrapped
Include following steps：
(1) extract：The carapace of Shrimp waste is opened, its whole stomach is taken out, stomach is cut off with surgical scissors and takes out gastric mill；
(2) clean：Gastric mill is cut off with surgical scissors and is deployed, it is with clear water that gastric mill surface clean is clean；
(3) connective tissue is removed：The connective tissue being connected with gastric mill bone is rejected with dissecting scissors and tip tweezers to reject；
(4) preserve：The gastric mill that will be handled through step (3) freezen protective at -20 DEG C.
3. according to the method described in claim 1, it is characterised in that the growth line counting step in the step (8) is：
(1) picture making：Section will be made to be placed on slide, supernatant water is dripped with glue head dropper；It is being connected with the micro- of CCD
Mirror 100 × times under the growth line of gastric mill is taken pictures, picture mosaic processing is carried out respectively to the picture clapped with image processing software；
(2) growth line is counted：Number of lines counting is entered to the growth line of calcified layer to the image of 100 × times lower collection.
4. the method according to claim any one of 1-3, material is used as in step (1) with wing ossicula cardia or middle ossicula cardia
Ground flat is chosen, embedding, cutting, fixed, polishing, polishing is then completed and implements subsequent step.
5. the method according to claim any one of 1-3, it is characterised in that：Described grinding tool is plastic mould.
6. the method according to claim any one of 1-3, it is characterised in that：The acryl powder and liquid curing-agent are matched somebody with somebody
It is 1 to put volume ratio:1-1:1.5.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710564807.1A CN107328778A (en) | 2017-07-12 | 2017-07-12 | A kind of method that utilization gastric mill identifies the Shrimp waste age |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710564807.1A CN107328778A (en) | 2017-07-12 | 2017-07-12 | A kind of method that utilization gastric mill identifies the Shrimp waste age |
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| CN107328778A true CN107328778A (en) | 2017-11-07 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110702483A (en) * | 2019-11-07 | 2020-01-17 | 上海海洋大学 | Pretreatment method for identifying seafood or meat at ultra-fast speed |
| CN111693527A (en) * | 2020-06-05 | 2020-09-22 | 上海海洋大学 | Method for identifying day age of cuttlefish by using cuttlefish bone |
| CN114740003A (en) * | 2022-04-24 | 2022-07-12 | 烟台大学 | Method for rapidly identifying age of crustacean |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645150A (en) * | 2016-11-25 | 2017-05-10 | 上海海洋大学 | Method for determining ages of shrimps and crabs by using eyestalk |
-
2017
- 2017-07-12 CN CN201710564807.1A patent/CN107328778A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645150A (en) * | 2016-11-25 | 2017-05-10 | 上海海洋大学 | Method for determining ages of shrimps and crabs by using eyestalk |
Non-Patent Citations (1)
| Title |
|---|
| RAOUF KILADA 等: "Direct determination of age in shrimps, crabs,and lobsters", 《CAN. J. FISH. AQUAT. SCI.》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110702483A (en) * | 2019-11-07 | 2020-01-17 | 上海海洋大学 | Pretreatment method for identifying seafood or meat at ultra-fast speed |
| CN110702483B (en) * | 2019-11-07 | 2022-05-10 | 上海海洋大学 | Pretreatment method for identifying seafood or meat at ultra-fast speed |
| CN111693527A (en) * | 2020-06-05 | 2020-09-22 | 上海海洋大学 | Method for identifying day age of cuttlefish by using cuttlefish bone |
| CN114740003A (en) * | 2022-04-24 | 2022-07-12 | 烟台大学 | Method for rapidly identifying age of crustacean |
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Application publication date: 20171107 |