CN110243640B

CN110243640B - Method for quickly preparing inner cavity grinding disc of deep sea porophytes

Info

Publication number: CN110243640B
Application number: CN201810189076.1A
Authority: CN
Inventors: 类彦立; 李铁刚
Original assignee: Institute of Oceanology of CAS; First Institute of Oceanography SOA
Current assignee: Institute of Oceanology of CAS; First Institute of Oceanography SOA
Priority date: 2018-03-08
Filing date: 2018-03-08
Publication date: 2022-01-25
Anticipated expiration: 2038-03-08
Also published as: CN110243640A

Abstract

The invention relates to the disciplines of marine biology, ancient oceanology, sedimentary geology and the like, can be applied to the fields of species identification research of polypore species, research of marine environment indication, ancient environment reconstruction, global change research and the like, and particularly relates to a rapid preparation method of a deep-sea polypore inner cavity structure abrasive disc. And (3) carrying out gum leaching cavity, hanging drop embedding, positioning fixing and water grinding treatment on the pore worms separated from the deep sea sediments, namely quickly obtaining the complete grinding disc of the deep sea pore worm inner cavity structure. The invention can more accurately identify the species of the holed worms by the layer information obtained by grinding the plates, and avoids scientific confusion such as identification errors caused by external morphological variations such as the appearance, the texture, the number of rooms and the like of the worm body. The invention provides accurate scientific basis and rapid problem solving scheme for accurately identifying the type of deep-sea porogens and researching marine environment indication thereof, and the subject fields of ancient environment reconstruction research, global change research and the like.

Description

Method for quickly preparing inner cavity grinding disc of deep sea porophytes

Technical Field

The invention relates to the disciplines of marine biology, ancient oceanology, sedimentary geology and the like, can be applied to the fields of species identification research of polypore species, research of marine environment indication, ancient environment reconstruction, global change research and the like, and particularly relates to a rapid preparation method of a deep-sea polypore inner cavity structure abrasive disc.

Background

The traditional method for preparing the abrasive disc is completely not suitable for researching deep sea porogens with thin and fragile shells, is completely different from the method in various aspects such as operation steps, chemical materials and experimental tools required by experiments, and has the defects of complicated experimental operation contents, long time consumption (1-3 months) in the experimental process, low success rate, easy breakage of samples, experimental failure and the like. The specific working procedure of the conventional grinding sheet is as follows:

(1) the process needs to be carried out in advance for tabletting and glue preparation, and adopts a liquid glue material to carry out glue baking, so that the process is time-consuming and labor-consuming, the local aging of the glue is easily caused, the glue baking process is difficult to control, and the curing degree of the glue needs to be detected at any time.

(2) In the process of burying fossil, the inside of a holed worm specimen is hollow easily, and the specimen is broken in the process of grinding and turning; in addition, because the glue solution viscosity is high when the glue is baked by the traditional method, bubbles in the specimen are difficult to be completely discharged at one time, the steps of repeating the steps for multiple times, manually breaking the bubbles by using a dissecting needle, heating again after the glue is completely solidified, and repeating the steps for multiple times; in addition, some bubbles can be additionally brought into the glue solution in the turning process, so that fossil observation and identification results are influenced; if the baking glue is too old, a large amount of bubbles are caused, the specimen is broken in the grinding process, and the experiment fails.

(3) In the abrasive disc and the mounting process, because traditional method buries fossil after baking glue earlier, the colloid sclerosis becomes fragile after solidifying, and elasticity diminishes, often leads to "falling the sediment" during the sample abrasive disc, and the colloid takes place when the structure fracture together with the sample casing, leads to the sample incomplete even experiment failure.

Disclosure of Invention

The invention aims to provide a preparation method of a grinding plate with a deep-sea holed insect inner cavity structure.

In order to achieve the purpose, the invention adopts the technical scheme that:

a method for quickly preparing the grinding disc with internal cavity of deep-sea holed worm includes such steps as immersing the deep-sea holed worm in gum, embedding in hanging drops, fixing by regulating position, and grinding in water.

The method specifically comprises the following steps: sticking the holed insects on a glass slide, heating and drying the glass slide above an alcohol lamp (the temperature is 100-150 ℃) to dry the insides of the holed insect cavities on the glass slide. Solid gum particles are adopted to surround the worm body, and the glass slide is heated to disperse bubbles generated by the sol until the gum melts and infiltrates the worm body, so that a 'gum infiltration cavity' is completed. And secondly, turning the glass slide 180 degrees for reverse heating, and forming hanging drops of the insect bodies in the melted gum to finish 'hanging drop embedding'. And thirdly, after reverse heating, turning the glass slide forward, gradually cooling and solidifying, adjusting the position of the holed worm during the process, and completely condensing and fixing the rubber-covered surface of the worm on the glass slide to finish 'positioning and fixing'. Dripping water on the grindstone, grinding the pest body glue-coated surface on the glass slide, and checking the glass slide by a microscope to master the grinding degree until the pest body is ground to the exposed initial room and the required layer surface, wherein the pest body does not need to be turned over in the period, thus finishing the water grinding piece treatment and finishing the manufacturing of the whole grinding piece.

The application of the deep-sea holed insect inner cavity structure abrasive disc in identifying deep-sea holed insects is provided.

The invention has the advantages that:

in the method, the deep-sea porogens live for a long time and in ocean sediment or deep-sea sediment, the samples of the deep-sea porogens are extremely precious due to difficult collection and extremely high voyage cost. In addition, the texture of the deep sea porogens is thin and crisp due to the growth environment and hydrological characteristics of the deep sea, and the specimens are very rare. In the traditional experiment treatment method, the hard fossil of the shell is taken as the technical background, so that the deep sea specimen is easy to crack and damage, and cannot be identified because the deep sea specimen cannot acquire complete internal structure information. The high experimental failure rate not only causes great waste on scientific research expenses, but also hinders scientific research and influences final scientific achievements. Compared with the traditional experimental method, the method of the invention saves time and labor, can finish the process within ten minutes, has high lapping success rate, clear imaging under a microscope, shortens the experimental period, saves the scientific research expenditure, and provides accurate scientific basis for identification; the method specifically comprises the following steps:

firstly, no impurity bubble interference exists. The method comprises the steps of firstly separating the porogens in the deep sea sediments, sticking the porogens specimen by using a writing brush, placing the specimen on a glass slide, slightly heating the specimen above an alcohol lamp, and drying the interior of a cavity. If utilize traditional approach directly to put into the gluey embedding with fossil sample, the inside moisture of sample slightly can produce a large amount of bubbles when gluey melts the embedding polypide, and on the one hand glue can not be complete embedding sample, easily causes the polypide to break during the abrasive disc, and on the other hand cavity is inside can produce a large amount of bubbles to because poroid shell is extremely fragile (the average 0.03 millimeter of shell wall thickness), the shell structure of polypide is often damaged in the process of traditional approach repeated baking and condensation. The imaging is fuzzy when the specimen is observed under a microscope, and the specimen identification result is influenced.

And secondly, the contract degree of the gum and the deep-sea holed worm specimen is high. The glue solution used in the traditional experimental method is suitable for fossil varieties, and because the fossil is fine in texture and hard in shell, the glue solution is baked to be cured and then melted to embed the fossil, and the glue is hard and is attached to the fossil. The deep sea holed worm related by the invention has a thin and crisp shell wall and a soft inner cavity, if the deep sea holed worm is embedded by using a traditional glue solution, the glue solution is hard after being cured, the inner cavity of the deep sea holed worm cannot be fully infiltrated, the supporting force of the inner cavity is insufficient, the colloid is broken easily due to the over-hard outer part during grinding, the specimen is deformed easily due to the insufficient supporting force of the inner cavity, and the final identification result is influenced. The gum of the invention has flexible texture, the density of the gum is similar to that of the shell of the deep sea polypide, the gum is dried by heating for one time slightly, the inner cavity and the shell embedded by the polypide are completely matched with the gum, the internal support is firm and keeps proper elasticity, the gum is not crisp, and the internal structure support is powerful. The sample is difficult damaged, and the shape is outstanding, does not fracture during the abrasive disc, and the sample is firm and the save time is of a specified duration, thereby practices thrift sample collection and research expenditure.

Thirdly, the process is simple and the time consumption of work is saved. The traditional experimental method is that fossil is immersed into glue after the glue is melted by heating. During the process, the dissecting needle is used for adjusting the direction of the fossil to discharge air bubbles or the dissecting needle is used for puncturing the air bubbles until the fossil is completely embedded into the glue. The steps are time-consuming and labor-consuming, the texture of the deep-sea holed worms is thin and crisp, and the specimen is easy to damage when the bubbles are punctured by an dissecting needle. The method of the invention adopts a proper amount of gum particles to surround the insect body and dissipates bubbles with slight heat. The gum is gradually heated on the alcohol lamp to gradually melt and infiltrate the insect body. Turning over and heating the upper part, and fully discharging bubbles by an alcohol lamp at the temperature of 100-150 ℃ to form hanging drops. Microscopic examination is carried out, and the position of the cavity of the holed insect is adjusted by a burning planing needle to be successfully embedded. According to the invention, the porous insect specimen is directly embedded by using the gum after being dried, so that no redundant bubbles are generated in the cavity of the insect body, the insect body is directly infiltrated when the gum is melted, the colloid is more attached to the insect body, the experimental process is rapid, the treatment method is time-saving and labor-saving, the experiment can be completed in a short time, and the research time is greatly shortened.

And fourthly, avoiding the specimen from cracking. After being embedded, the insect body is turned over, the glass slide is cooled and solidified, and the position of the holed insect is adjusted in the process, so that an ideal observation direction and an angle convenient for grinding are obtained, the positioning and drying are finished, and the holed insect is placed on a daily experiment table and naturally cooled for 2 minutes. The step is to avoid the slide glass from cracking when contacting low-temperature materials such as grindstones and the like after being heated at high temperature. The cooling step is not specifically described in the conventional experimental methods.

Fifthly, the imaging effect is clear. After the glass slide is slowly cooled and solidified, a little water is dripped on a grinding stone, the glass slide is lightly ground, and the grinding degree is mastered through microscopic examination until the glass slide is ground to the exposed initial room and the required layer. The traditional experimental method is to put the abrasive on a glass plate and add water to the piece embedded with the fossil for grinding. When the stone is ground to the initial room, the stone is cleaned, wiped dry, heated again to soften the glue, and then the stone is turned over. And (3) tightly attaching the ground surface of the fossil to the glass slide, grinding again after the glue is completely cooled, and finally cleaning the pieces and numbering. The step is not only complicated, but also requires skilled manipulation of operators, the sample has high breakage rate, impurities are often mixed in the adjusting process, interference of different degrees is caused to the inner cavity structure of the sample, and imaging blurring under a microscope is realized. The method provided by the invention is simple to operate, high in grinding success rate, free of impurity interference in the grinding process, good in grinding specimen effect, clear in imaging under a microscope, and provides a powerful basis for identification and research.

Drawings

Fig. 1 is a schematic diagram of a method for rapidly manufacturing an inner cavity grinding plate for deep-sea porogens provided by an embodiment of the present invention.

Fig. 2 is a schematic front profile view of a deep-sea porous insect grinding plate according to an embodiment of the invention.

Figure 3 is a schematic front top surface profile of a deep sea porous insect grinding plate according to an embodiment of the present invention.

Fig. 4 is a schematic structural view of an inner cavity of the deep-sea holed insect grinding plate provided by the embodiment of the invention.

Detailed Description

The following examples are further illustrative of the present invention and are not intended to be limiting thereof.

The invention aims to obtain a complete internal structure grinding disc for the individual tiny deep-sea poromydia with thin and crisp shells and realize accurate species identification. According to the invention, a deep-sea holed worm specimen is obtained through separation, then gum dipping cavity, hanging drop embedding, positioning drying and water grinding sheet treatment are carried out on the specimen, and the internal structure of the deep-sea holed worm is judged according to the ground layer. The invention can more accurately identify the species of the holed insects by the layer information obtained by grinding the plates, and avoids the identification errors caused by external factors such as the appearance, the texture, the number of rooms and the like of the insects. The invention provides accurate scientific basis for accurately identifying the types of the porogens, researching the marine environment indication and researching the global change.

The invention can more accurately identify the species of the holed worms by the layer information obtained by grinding the plates, and avoids scientific confusion such as identification errors caused by external morphological variations such as the appearance, the texture, the number of rooms and the like of the worm body. The invention provides accurate scientific basis and rapid problem solving scheme for accurately identifying the type of deep-sea porogens and researching marine environment indication thereof, and the subject fields of ancient environment reconstruction research, global change research and the like.

Example 1

1. Tool: alcohol burner, solid gum, dissecting needle (medical skin test needle), fine grindstone, glass slide, and fine brush pen.

2. The method comprises the steps of extracting a deep sea holed insect sample, sticking the holed insect on a glass slide by using a writing brush, heating the sample by using an alcohol lamp (slightly lifting the sample above the alcohol lamp), drying the sample, and otherwise, affecting gum dipping.

3. And (3) taking gum particles (the particle size is from rice grains to soybean grains), embedding the porose on the glass slide, namely surrounding the polypide with the gum and enabling bubbles in the gum to escape by slight heating on an alcohol lamp near the polypide.

4. Heating with alcohol lamp at 100-150 deg.C to melt gum, soaking in insect, and heating too fast to prevent the gum from aging and cracking easily. If the pectin discolors, the heating is excessive, so that the aged product is fragile and can not be ground any more. The air bubbles are easy to discharge when the upper part is turned and heated, and the formed hanging drop is convenient to grind.

5. Microscopic examination, adjusting the body position with a burning and hot planning needle, and if the glue is spread out too much, the hot needle can be used for one time.

6. After the poriferous insects are embedded, if the room temperature is low, the glass slide is not required to be directly placed on a table top of a metal porcelain and the like, so that the glue is prevented from being cracked due to too fast temperature reduction. About 5-8 minutes, the temperature is reduced to room temperature and after solidification, grinding is ready.

7. Dripping a little water on the grindstone, grinding lightly, checking with microscope to control grinding degree, washing with water absorbed by Pasteur pipette, and grinding continuously until the required layer is ground.

8. Note that: the alcohol lamp uses small fire in the whole process.

As can be seen from the attached drawings, fig. 2 and fig. 3 are external contour diagrams before grinding discs for deep sea holed insects, related literature data are consulted, the appearances of more than dozens of kinds of holed insects are similar to the external contour diagrams, the schematic diagram of the inner cavity structure of fig. 4 is obtained after specific experimental treatment of the inner cavity grinding discs, and through comparison with the literature data, the similarities between the external contour and the inner cavity structure and the common looper Spiroloculina commu Cushman & Todd,1944 reported in east seabed geology are both 99.99%, so that the identification result is as follows: the common armyworm Spiroloculina communis Cushman & Todd, 1944.

Claims

1. A method for quickly preparing an inner cavity grinding plate of deep sea porogens,

sticking the holed worms on a glass slide, and drying at the temperature of 100-150 ℃ to dry the insides of the holed worm cavities on the glass slide; solid gum particles are adopted to surround the worm body, and the glass slide is heated to disperse bubbles generated by the sol until the gum melts and infiltrates the worm body, so that a 'gum infiltration cavity' is completed;

overturning the glass slide for 180 ℃ to perform reverse heating to complete hanging drop embedding;

righting the glass slide after reverse heating, cooling and solidifying, adjusting the position of the holed worm during the process, and completely condensing and fixing the glue-coated surface of the worm on the glass slide to finish 'positioning and fixing';

dripping water on the grindstone, grinding the pest body glue-coated surface on the glass slide, and checking the glass slide by a microscope to master the grinding degree until the pest body is ground to the exposed initial room and the required layer surface, wherein the pest body does not need to be turned over in the period, thus finishing the water grinding piece treatment and finishing the manufacturing of the whole grinding piece.

2. Use of a deep-sea holed insect intracavity structured abrasive disc obtained by the method of claim 1 for identifying deep-sea holed insects.