CN104293765A - Preparation method of solid photosynthetic bacterium - Google Patents
Preparation method of solid photosynthetic bacterium Download PDFInfo
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- CN104293765A CN104293765A CN201410473548.8A CN201410473548A CN104293765A CN 104293765 A CN104293765 A CN 104293765A CN 201410473548 A CN201410473548 A CN 201410473548A CN 104293765 A CN104293765 A CN 104293765A
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- photosynthetic bacterium
- absorbent resin
- bacterial classification
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Abstract
The invention relates to a preparation method of a solid photosynthetic bacterium. The preparation method comprises the specific following steps: firstly amplifying the photosynthetic bacterium selected as a vector, absorbing the strain with a super absorbent resin selected as a solidifying agent and solidifying together to obtain the solid photosynthetic bacterium strain. Through the implementation of the preparation method disclosed by the invention, the prepared solid photosynthetic bacterium strain is not easily infected with heterogeneous bacteria, is stable, has good quality and can be widely in fields such as farming, aquaculture, food processing and environmental protection. The storage period and quality guarantee period of the prepared solid photosynthetic bacterium product are greatly prolonged. Meanwhile, the prepared solid photosynthetic bacterium is very convenient to transport and circulate. During the implementation of the preparation method, super absorbent resin with relatively high water absorption capacity is applied as the solidifying agent, the consumption of the super absorbent resin is very less and thus the produced product is low in production cost, the application effect is good and the preparation method is worthy of being widely promoted.
Description
Technical field
The present invention relates to a kind of microbial preparation field, be specifically related to the preparation method of a kind of solid-state shape photosynthetic bacterium.
Technical background
Photosynthetic bacterium, the prokaryotic organism with original luminous energy synthetic system the most ancient on the earth, photosynthetic bacterium is distributed widely in marsh, pond, lake, river, ditch, ocean and soil, restrain oneself high temperature, high salt and low temperature, less salt ability strong especially, even in the hot spring of 90 DEG C of high temperature, containing in the salt lake of 30% salinity, in the deep-sea deeply reaching 2000 meters, on the seashore that the South Pole is ice-bound, the trace of photosynthetic bacterium can be found.
The history of the action oriented research of photosynthetic bacterium and productivity research existing 30 or 40 years.Japanology must comparatively early, and the sixties in 20th century, the change of bacterium number when Japanese Scientists has observed the fecal sewage of high density naturally place, is first disclosed natural organic sewage and be cleaned by the ecological succession of microorganism.Through further research, find that again the young of the thalline of photosynthetic bacterium to poultry, domestic animal and fish, shrimp, crab, shellfish has obvious growth promoting effects and improve the effect of surviving rate, thus opened up new field for the comprehensive utilization of photosynthetic bacterial thallus.
The research of China to photosynthetic bacterium is started late, be only limitted at first bacterial classification separation and for high-concentration sewage process, many professionals had carried out again otherwise applied research afterwards, achieved a large amount of achievements.The people such as Shanghai Aquatic Products Univ. 9CN) Zhang Daonan in 1987 apply it in shrimps nursery, and result test group comparatively control group surviving rate improves 19.3%, and distortion ratio improves 18.5%.Now, photosynthetic bacterium as a kind of most attention that there is special nutrition, growth promotion, disease-resistant factor and high-level efficiency purifying cultivation sewage and environment and the nontoxic Special Bacteria of aquatic animal are received to people with like.Therefore, it is widely used in the field that aquaculture, livestock and poultry cultivation and water treatment are relevant.
At present, the photosynthetic bacterium product on market is various, and in these photosynthetic bacterium products, and production technology process is especially in variation, and most products is all liquid, there is inconvenience in the storage of product, the transport process of circulation.
Application number is " solid photosynthetic bacterial technology " of 94118491.9, this invention proposes with the pollution-free soil of deep layer as base-material, and be aided with light calcium carbonate and syrup, extractum carnis, yeast extract paste, the soluble nutrient such as vitamin B complexes and plurality of inorganic salt, formation of solid polycomplex substratum according to a certain ratio, the liquid photosynthetic bacterium that ordinary method is cultivated is inoculated after individually sterilizing, use shallow-layer or thin solid zymotechnique, 48-72 hour is cultivated under be 1000-1200lux and temperature being 26-30 DEG C of condition in illumination, and drying process and pulverizing and obtain solid photosynthetic bacteria preparation.
Application number is " solid photosynthetic bacteria " of 98119850.3, and powdered rice hulls non-woven fabrics and vibratory liquefaction low temperature drying technology are selected in this invention, and make thalline dormancy under not deactivated condition, the solid photosynthetic bacteria finished product quality guaranteed period of making can reach more than 12 months.
Application number is " process for fixation of photosynthetic bacterium " of 200610018362.9, this invention selects granularity to be 120 order ~ 200 object zeolite powders, be in mass ratio 1: 0.03 ~ 0.05: 0.2 ~ 0.3 ratio add the sodium alginate and photosynthetic bacterium concentrated solution that viscosity is 500-1000mpas, mix and mix thoroughly, granulate, being placed in concentration is that the CaCl2 solution of 20g/L ~ 40g/L hardens, after clear water rinsing finished product.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art and the preparation method that brand-new a kind of solid-state shape photosynthetic bacterium is provided.Specifically comprise: select photosynthetic bacterium to do carrier, select super absorbent resin as solidifying agent, with super absorbent resin bacterial classification absorbing and solidified again after first being expanded by photosynthetic bacterium becomes a kind of solid-state shape photosynthetic bacterium bacterial classification.By implementing the present invention, prepared solid photosynthetic bacterium bacterial classification is out not easy bacteria infection, bacteriological quality is good, stable, can be widely used in the fields such as plant husbandry, aquaculture, food-processing, environmental protection.The solid photosynthetic bacterium product prepared significantly extends on storage, quality guaranteed period.Meanwhile, the solid photosynthetic bacterium prepared is all very convenient in the middle of the transport process of circulation.Implement the present invention, apply the higher High hydrophilous resin of Water absorption as solidifying agent, consumption is few, so the products production cost produced is low, but effect is good, is worth promoting.
The present invention is achieved through the following technical solutions:
A preparation method for solid-state shape photosynthetic bacterium, technical scheme comprises: select photosynthetic bacterium to do carrier, selects super absorbent resin as solidifying agent, to be absorbed by bacterial classification again and solidify after first being expanded by photosynthetic bacterium with super absorbent resin.
Specifically comprise the following steps:
1, bacterial classification collection: use and select color light violet red, concentration is that the liquid photosynthetic bacterium finished product of 3,000,000,000/milliliter does female kind.
2, solidifying agent collection: select Water absorption to be that the super absorbent resin of 200 ~ 300 times is as solidifying agent.
3, bacterial classification is expanded: raw material is that mother plants, 3 kilograms of brown sugar, 100 grams of potassium primary phosphates, 30 grams of peptones, 50 kilograms of clear water form by 1 kilogram of photosynthetic bacterium, first above-mentioned clear water is positioned in the plastic tank of white clear, then in well water, brown sugar, potassium primary phosphate, peptone is being added successively, and by stirring, brown sugar, potassium primary phosphate, peptone are dissolved in clear water completely, then plastic tank is placed in visible ray, and in the place in cool place, bung is sealed and carries out anaerobically fermenting and expand to obtain bacterial classification in 10 ~ 15 days.
4, solidify: super absorbent resin is rendered in bacterial classification by the ratio in 1: 200 ~ 300, and after 20 ~ 30 minutes, bacterial classification absorbs and solidifies by super absorbent resin becomes solid-state shape.
5, pack: the photosynthetic bacterium bacterial classification of solid-state shape is wired up into finished product according to quantity.
The present invention both had the following advantages:
1, solid-state shape photosynthetic bacterium its preparation method proposed by the invention is not only novel, and preparation technology simply, easily implements.
2, by implementing the present invention, prepared solid photosynthetic bacterium bacterial classification is out not easy bacteria infection, bacteriological quality is good, stable, can be widely used in the fields such as plant husbandry, aquaculture, food-processing, environmental protection.
3, by implementing the present invention, the solid photosynthetic bacterium product prepared significantly extends on storage, quality guaranteed period.
4, by implementing the present invention, the solid photosynthetic bacterium prepared is all very convenient in the middle of the transport process of circulation.
5, implement the present invention flexible, apply the higher High hydrophilous resin of Water absorption as solidifying agent, consumption is few, so the products production cost produced is low, but effect is good, is worth promoting.
Embodiment
Below in conjunction with embodiment, method of the present invention is further illustrated.
A preparation method for solid-state shape photosynthetic bacterium, technical scheme comprises: select photosynthetic bacterium to do carrier, selects super absorbent resin as solidifying agent, to be absorbed by bacterial classification again and solidify after first being expanded by photosynthetic bacterium with super absorbent resin.
Embodiment is as follows:
1, bacterial classification collection: use and select color light violet red, concentration is that the liquid photosynthetic bacterium finished product of 3,000,000,000/milliliter does female kind.
2, solidifying agent collection: select Water absorption to be that the super absorbent resin of 200 ~ 300 times is as solidifying agent.
3, bacterial classification is expanded: raw material is that mother plants, 3 kilograms of brown sugar, 100 grams of potassium primary phosphates, 30 grams of peptones, 50 kilograms of clear water form by 1 kilogram of photosynthetic bacterium, first above-mentioned clear water is positioned in the plastic tank of white clear, then in well water, brown sugar, potassium primary phosphate, peptone is being added successively, and by stirring, brown sugar, potassium primary phosphate, peptone are dissolved in clear water completely, then plastic tank is placed in visible ray, and in the place in cool place, bung is sealed and carries out anaerobically fermenting and expand to obtain bacterial classification in 10 ~ 15 days.
4, solidify: super absorbent resin is rendered in bacterial classification by the ratio in 1: 200 ~ 300, and after 20 ~ 30 minutes, bacterial classification absorbs and solidifies by super absorbent resin becomes solid-state shape.
5, pack: the photosynthetic bacterium bacterial classification of solid-state shape is wired up into finished product according to quantity.
Claims (1)
1. a preparation method for solid-state shape photosynthetic bacterium, its feature comprises: select photosynthetic bacterium to do carrier, selects super absorbent resin as solidifying agent, to be absorbed by bacterial classification again and solidify after first being expanded by photosynthetic bacterium with super absorbent resin;
Specifically comprise the following steps:
(1) bacterial classification collection: use and select color light violet red, concentration is that the liquid photosynthetic bacterium finished product of 3,000,000,000/milliliter does female kind;
(2) solidifying agent collection: select Water absorption to be that the super absorbent resin of 200 ~ 300 times is as solidifying agent;
(3) bacterial classification is expanded: raw material is that mother plants, 3 kilograms of brown sugar, 100 grams of potassium primary phosphates, 30 grams of peptones, 50 kilograms of clear water form by 1 kilogram of photosynthetic bacterium, first above-mentioned clear water is positioned in the plastic tank of white clear, then in well water, brown sugar, potassium primary phosphate, peptone is being added successively, and by stirring, brown sugar, potassium primary phosphate, peptone are dissolved in clear water completely, then plastic tank is placed in visible ray, and in the place in cool place, bung is sealed and carries out anaerobically fermenting and expand to obtain bacterial classification in 10 ~ 15 days;
(4) solidify: super absorbent resin is rendered in bacterial classification by the ratio in 1: 200 ~ 300, and after 20 ~ 30 minutes, bacterial classification absorbs and solidifies by super absorbent resin becomes solid-state shape;
(5) pack: the photosynthetic bacterium bacterial classification of solid-state shape is wired up into finished product according to quantity.
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| CN201410473548.8A CN104293765A (en) | 2014-09-17 | 2014-09-17 | Preparation method of solid photosynthetic bacterium |
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| CN201410473548.8A CN104293765A (en) | 2014-09-17 | 2014-09-17 | Preparation method of solid photosynthetic bacterium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104845907A (en) * | 2015-04-16 | 2015-08-19 | 赵宏耀 | Preparation of photosynthetic bacteria solid strain with conelike redpepper stalks and leaves as raw materials |
| CN105063004A (en) * | 2015-07-31 | 2015-11-18 | 黄旭胜 | Solid probiotic |
Citations (6)
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|---|---|---|---|---|
| CN1124293A (en) * | 1994-12-09 | 1996-06-12 | 王厚德 | Solid photosynthetic bacteria technology |
| CN1221791A (en) * | 1998-09-22 | 1999-07-07 | 庞昆 | Solistate photosynthetic germ |
| CN1696276A (en) * | 2004-05-13 | 2005-11-16 | 韩舞鹰 | Twol steps continuous fermentation technology for beneficial microbe (EM) to improve water quality |
| CN103255122A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Preparation method of solid photosynthetic bacterium |
| CN103283605A (en) * | 2013-06-17 | 2013-09-11 | 何寒 | Preparation method of tissue culture medium without agar |
| CN103329800A (en) * | 2013-06-17 | 2013-10-02 | 何寒 | Tissue culture medium without agar during plant open tissue culturing and preparation method of tissue culture medium |
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2014
- 2014-09-17 CN CN201410473548.8A patent/CN104293765A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1124293A (en) * | 1994-12-09 | 1996-06-12 | 王厚德 | Solid photosynthetic bacteria technology |
| CN1221791A (en) * | 1998-09-22 | 1999-07-07 | 庞昆 | Solistate photosynthetic germ |
| CN1696276A (en) * | 2004-05-13 | 2005-11-16 | 韩舞鹰 | Twol steps continuous fermentation technology for beneficial microbe (EM) to improve water quality |
| CN103255122A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Preparation method of solid photosynthetic bacterium |
| CN103283605A (en) * | 2013-06-17 | 2013-09-11 | 何寒 | Preparation method of tissue culture medium without agar |
| CN103329800A (en) * | 2013-06-17 | 2013-10-02 | 何寒 | Tissue culture medium without agar during plant open tissue culturing and preparation method of tissue culture medium |
Non-Patent Citations (1)
| Title |
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| 吴清平 等: "微生物固体培养基凝固剂研究进展", 《微生物学通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104845907A (en) * | 2015-04-16 | 2015-08-19 | 赵宏耀 | Preparation of photosynthetic bacteria solid strain with conelike redpepper stalks and leaves as raw materials |
| CN105063004A (en) * | 2015-07-31 | 2015-11-18 | 黄旭胜 | Solid probiotic |
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Application publication date: 20150121 |