CN104292353A - Anti-oxidation natural plant polysaccharide and preparation method thereof - Google Patents
Anti-oxidation natural plant polysaccharide and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the field of traditional Chinese medicines, and in particular relates to anti-oxidation natural plant polysaccharide. According to the anti-oxidation natural plant polysaccharide, natural traditional Chinese herbal medicines such as oldenlandia diffusa, sculellaria barbata and herba epimedii are taken as core components, the medical effect components polysaccharide of the components are extracted, and through scientific and reasonable compatibility, a formula with the optimal anti-oxidation property is achieved, and the anti-oxidation natural plant polysaccharide has the synergism effect of synergetic complementation of the polysaccharide of the three medicines. The polysaccharide of the formula is extracted from natural plants, is free of chemical synthetic component, is free of hormone, heavy metal or any western medicine component, has relatively high medical functions such as anti-oxidation, anti-aging and immunity improvement, and is very high in industrialization development potential.
Description
Technical field
The invention belongs to the field of Chinese medicines, be specifically related to a kind of anti-oxidant natural plant polyose.
Background technology
In recent years, along with the change of society, life, work, environment, human diseases spectrum there occurs huge change.The disease that incidence is high and harm is maximum has turned to new hair style variability virus, body aging or body own metabolism by traditional infectious single factor test disease and has regulated and controled the not normal complexity multifactor property disease for main spectrum group.Under this trend, numerous conventional medicament that is target with single disease target spot more and more can not meet the needs of mankind's preventing and treating diseases.Therefore, from enhancing body oxidation-resistance and autoimmunity, utilize the Integrate adjustment ability of the distinctive multicomponent of Chinese herb medicine, too many levels, Mutiple Targets to flight against senium of human body anti-oxidant and immunity system improve, thus reach the object improving human health level, become a focus in medical treatment or health care activity.
Modern medicine study achievement shows, a lot of herbal medicine contains polyose, organic acid, alkaloids, glucoside and volatile oil etc., these materials pass through the resistance of oxidation promoting body in human body, strengthen immune cell function, improve the effects such as cytokine secretion, the consolidation to each organ dysfunction of body and lifting can be realized, the control of disease is had great significance.
Herba Scutellariae Barbatae, Spreading Hedyotis Herb and Herba Epimedii are the primary medicinal plants of Funiu Mountains of Henan Province In China.Medical book of reportedly uniting is recorded, and this three herbal medicine has invigorating the spleen and replenishing QI, promoting blood circulation and removing blood stasis, clearing heat and detoxicating effect, as reasonable compatibility uses, when playing an important role in anti-oxidant and immune lifting.By modern science bibliographical information, Scutellaria Barbata D. Don Polysaccharides can promote that the mouse boosting cell lymphocyte transformation that ConA induces, its optimal concentration are 400 μ g/ml in vitro.Subcutaneous administrations one Zhou Houke significantly improves the percentage of esterase positive cell in Mouse Peripheral Blood Lymphocyte, promotes the mouse delayed allergy that DNCB brings out after intraperitoneal administration.Herba Scutellariae Barbatae water extraction polysaccharide is to H
2o
2there is stronger scavenging(action), and in dose-effect relationship.Show through mouse experiment, its oxidation-resisting and caducity effect is by improving SOD vigor and resisting old and feeble prolongs life to the scavenging(action) of negative oxyradical and lipoid peroxidization resistant.It is active that Herba Hedyotidis Diffusae polysaccharide extract can strengthen immune phagocytic cell, very strong promoter action is had to the proliferation activity of mouse boosting cell, the generation and the monocytic cytokine that strengthen B cell antibody produce, strengthen monocyte to the phagocytic function of tumour cell, and promote the secretion of proliferation of bone marrow cells reaction and IL2.In addition, Herba Hedyotidis Diffusae polysaccharide has the effect of enhancing body specific immune function and non-specific immune function.Herba Epimedii can improve the phagocytic function of Turnover of Mouse Peritoneal Macrophages and the phagocytic function of the Turnover of Mouse Peritoneal Macrophages by endoxan damage can be made to return to normal level.Herba Epimedii has obvious enhancement to the phagocytic function of immunologic hypofunction model mice scavenger cell caused by heavy dose of hydrocortisone and hydroxyurea.Epimedium brevicornum polysaccharide has dual regulation to macrophages secrete IL-1 and tumour necrosis factor, and promotes the lymphocytic proliferation and differentiation of T/B and natural killer cell activity, improves Immune Function.In addition, Herba Epimedii can promote the secretion of panimmunity cytokine, as Interferon, rabbit and IL-2 etc.
In sum, tradition medicinal plant Herba Scutellariae Barbatae, no matter Spreading Hedyotis Herb and Herba Epimedii are based on Traditional Chinese medical theory, or modern pharmacology Research foundation, all show the potentiality of extremely strong anti-oxidant activity and enhancing body immunologic function, its chief active position is polysaccharide.Therefore, target optimized Separation Herba Scutellariae Barbatae, Spreading Hedyotis Herb and epimedium brevicornum polysaccharide, and carry out composite under drug effect instructs, a kind of compound prescription of effective anti-oxidant natural plant polyose can be realized.
Summary of the invention
The object of this invention is to provide a kind of anti-oxidant natural plant polyose, under the prerequisite of not adding any artificial synthetic chemistry composition, exploitation has natural plant polyose that is anti-oxidant, anti-aging and lifting immunizing power.
The present invention is for solving the problem, taked technical scheme be: a kind of anti-oxidant natural plant polyose, described vegetable polysaccharides is made up of Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide, and the weight ratio preparing each component needed for vegetable polysaccharides is 1:1:1;
Described Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide are that Spreading Hedyotis Herb, Herba Scutellariae Barbatae and Herba Epimedii are obtained Spreading Hedyotis Herb extracting solution, Herba Scutellariae Barbatae extracting solution and extraction ofHerba Epimedii respectively by ultrasonication, then Spreading Hedyotis Herb extracting solution, Herba Scutellariae Barbatae extracting solution and the further evaporate to dryness of extraction ofHerba Epimedii are obtained respectively.
The preparation method of described anti-oxidant natural plant polyose, comprises the following steps:
The preparation of step one, Herba Hedyotidis Diffusae polysaccharide
Spreading Hedyotis Herb after pulverizing and distilled water are pressed the weight ratio mixing of 1:14, be the ultrasonic extraction 30-40min of 40kHz by frequency afterwards, extracting solution aperture is the filter paper filtering of 20 μm, collect filtrate, repeat said extracted process 2-3 time, merging filtrate, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture I, the ethanol that volumetric concentration is 95% is added to mixture I, after making mixing, the volumetric concentration of ethanol is 60%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely Herba Hedyotidis Diffusae polysaccharide is obtained by after filtrate evaporate to dryness,
The preparation of step 2, Scutellaria Barbata D. Don Polysaccharides
Herba Scutellariae Barbatae after pulverizing and distilled water are mixed and stir, the distilled water volume that every gram of Herba Scutellariae Barbatae is corresponding is 14ml, be the ultrasonic extraction 10min of 40kHz by frequency afterwards, by the filter paper filtering that extracting solution aperture is 20 μm, collect filtrate, repeat said extracted process 5-6 rear merging filtrate, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture II, the ethanol that concentration of volume percent is 95% is added to mixture II, after making mixing, the volumetric concentration of ethanol is 80%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely Scutellaria Barbata D. Don Polysaccharides is obtained by after filtrate evaporate to dryness,
The preparation of step 3, epimedium brevicornum polysaccharide
Herba Epimedii after pulverizing and distilled water are mixed and stir, the distilled water volume that every gram of Herba Epimedii is corresponding is 10ml, be the ultrasonic extraction 10min of 40kHz by frequency afterwards, by the filter paper filtering that extracting solution aperture is 20 μm, collect filtrate, repeat said extracted process 4-5 rear merging filtrate, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture III, the ethanol that volumetric concentration is 95% is added to mixture III, after making mixing, the volumetric concentration of ethanol is 70%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely epimedium brevicornum polysaccharide is obtained by after filtrate evaporate to dryness,
Step 4, preparation
Obtained Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide are mixed by the weight ratio of 1:1:1 and i.e. obtained vegetable polysaccharides after stirring.
beneficial effect
The present invention with natural Chinese medicinal herb Spreading Hedyotis Herb, Herba Scutellariae Barbatae and Herba Epimedii for core, its effective component Polyose extraction is extracted, and the formula of maximum resistance of oxidation is obtained through scientific and reasonable compatibility, the formula invented serves the synergism collaborative to three taste source medicine polysaccharide complementations.Polysaccharide component in formula derives from natural phant, and without chemosynthesis composition, without hormone, heavy metal or any western medicine composition, have the pharmacodynamic features such as stronger anti-oxidant, anti-ageing and immunizing power lifting, have industrialization development potentiality.
Embodiment
A kind of anti-oxidant natural plant polyose, described vegetable polysaccharides is made up of Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide, and the weight ratio preparing each component needed for vegetable polysaccharides is 1:1:1;
Described Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide are that Spreading Hedyotis Herb, Herba Scutellariae Barbatae and Herba Epimedii are obtained Spreading Hedyotis Herb extracting solution, Herba Scutellariae Barbatae extracting solution and extraction ofHerba Epimedii respectively by ultrasonication, then Spreading Hedyotis Herb extracting solution, Herba Scutellariae Barbatae extracting solution and the further evaporate to dryness of extraction ofHerba Epimedii are obtained respectively.
The preparation method of described anti-oxidant natural plant polyose, comprises the following steps:
The preparation of step one, Herba Hedyotidis Diffusae polysaccharide
Spreading Hedyotis Herb after pulverizing and distilled water are pressed the weight ratio mixing of 1:14, be the ultrasonic extraction 30-40min of 40kHz by frequency afterwards, extracting solution aperture is the filter paper filtering of 20 μm, collect filtrate, repeat said extracted process 2-3 time, merging filtrate, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture I, the ethanol that volumetric concentration is 95% is added to mixture I, after making mixing, the volumetric concentration of ethanol is 60%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely Herba Hedyotidis Diffusae polysaccharide is obtained by after filtrate evaporate to dryness,
The preparation of step 2, Scutellaria Barbata D. Don Polysaccharides
Herba Scutellariae Barbatae after pulverizing and distilled water are mixed and stir, the distilled water volume that every gram of Herba Scutellariae Barbatae is corresponding is 14ml, be the ultrasonic extraction 10min of 40kHz by frequency afterwards, by the filter paper filtering that extracting solution aperture is 20 μm, collect filtrate, repeat said extracted process 5-6 rear merging filtrate, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture II, the ethanol that concentration of volume percent is 95% is added to mixture II, after making mixing, the volumetric concentration of ethanol is 80%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely Scutellaria Barbata D. Don Polysaccharides is obtained by after filtrate evaporate to dryness,
The preparation of step 3, epimedium brevicornum polysaccharide
Herba Epimedii after pulverizing and distilled water are mixed and stir, the distilled water volume that every gram of Herba Epimedii is corresponding is 10ml, be the ultrasonic extraction 10min of 40kHz by frequency afterwards, by the filter paper filtering that extracting solution aperture is 20 μm, collect filtrate, repeat said extracted process 4-5 rear merging filtrate, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture III, the ethanol that volumetric concentration is 95% is added to mixture III, after making mixing, the volumetric concentration of ethanol is 70%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely epimedium brevicornum polysaccharide is obtained by after filtrate evaporate to dryness,
Step 4, preparation
Obtained Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide are mixed by the weight ratio of 1:1:1 and i.e. obtained vegetable polysaccharides after stirring.
Embodiment 1
A kind of anti-oxidant natural plant polyose, described vegetable polysaccharides is made up of Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide, and the weight ratio preparing each component needed for vegetable polysaccharides is 1:1:1;
Described Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide are that Spreading Hedyotis Herb, Herba Scutellariae Barbatae and Herba Epimedii are obtained Spreading Hedyotis Herb extracting solution, Herba Scutellariae Barbatae extracting solution and extraction ofHerba Epimedii respectively by ultrasonication, then Spreading Hedyotis Herb extracting solution, Herba Scutellariae Barbatae extracting solution and the further evaporate to dryness of extraction ofHerba Epimedii are obtained respectively.
The preparation method of described anti-oxidant natural plant polyose, comprises the following steps:
The preparation of step one, Herba Hedyotidis Diffusae polysaccharide
Spreading Hedyotis Herb after pulverizing and distilled water are pressed the weight ratio mixing of 1:14, be the ultrasonic extraction 30min of 40kHz by frequency afterwards, extracting solution aperture is the filter paper filtering of 20 μm, collect filtrate, repeat said extracted process 2 times, merging filtrate, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture I, the ethanol that volumetric concentration is 95% is added to mixture I, after making mixing, the volumetric concentration of ethanol is 60%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely Herba Hedyotidis Diffusae polysaccharide is obtained by after filtrate evaporate to dryness,
The preparation of step 2, Scutellaria Barbata D. Don Polysaccharides
Herba Scutellariae Barbatae after pulverizing and distilled water are mixed and stir, the distilled water volume that every gram of Herba Scutellariae Barbatae is corresponding is 14ml, be the ultrasonic extraction 10min of 40kHz by frequency afterwards, by the filter paper filtering that extracting solution aperture is 20 μm, collect filtrate, repeat merging filtrate after said extracted process 5 times, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture II, the ethanol that concentration of volume percent is 95% is added to mixture II, after making mixing, the volumetric concentration of ethanol is 80%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely Scutellaria Barbata D. Don Polysaccharides is obtained by after filtrate evaporate to dryness,
The preparation of step 3, epimedium brevicornum polysaccharide
Herba Epimedii after pulverizing and distilled water are mixed and stir, the distilled water volume that every gram of Herba Epimedii is corresponding is 10ml, be the ultrasonic extraction 10min of 40kHz by frequency afterwards, by the filter paper filtering that extracting solution aperture is 20 μm, collect filtrate, repeat said extracted process 4-5 rear merging filtrate, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture III, the ethanol that volumetric concentration is 95% is added to mixture III, after making mixing, the volumetric concentration of ethanol is 70%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely epimedium brevicornum polysaccharide is obtained by after filtrate evaporate to dryness,
Step 4, preparation
Obtained Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide are mixed by the weight ratio of 1:1:1 and i.e. obtained vegetable polysaccharides after stirring.
Embodiment 2
A kind of anti-oxidant natural plant polyose, described vegetable polysaccharides is made up of Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide, and the weight ratio preparing each component needed for vegetable polysaccharides is 1:1:1;
Described Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide are that Spreading Hedyotis Herb, Herba Scutellariae Barbatae and Herba Epimedii are obtained Spreading Hedyotis Herb extracting solution, Herba Scutellariae Barbatae extracting solution and extraction ofHerba Epimedii respectively by ultrasonication, then Spreading Hedyotis Herb extracting solution, Herba Scutellariae Barbatae extracting solution and the further evaporate to dryness of extraction ofHerba Epimedii are obtained respectively.
The preparation method of described anti-oxidant natural plant polyose, comprises the following steps:
The preparation of step one, Herba Hedyotidis Diffusae polysaccharide
Spreading Hedyotis Herb after pulverizing and distilled water are pressed the weight ratio mixing of 1:14, be the ultrasonic extraction 40min of 40kHz by frequency afterwards, extracting solution aperture is the filter paper filtering of 20 μm, collect filtrate, repeat said extracted process 3 times, merging filtrate, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture I, the ethanol that volumetric concentration is 95% is added to mixture I, after making mixing, the volumetric concentration of ethanol is 60%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely Herba Hedyotidis Diffusae polysaccharide is obtained by after filtrate evaporate to dryness,
The preparation of step 2, Scutellaria Barbata D. Don Polysaccharides
Herba Scutellariae Barbatae after pulverizing and distilled water are mixed and stir, the distilled water volume that every gram of Herba Scutellariae Barbatae is corresponding is 14ml, be the ultrasonic extraction 10min of 40kHz by frequency afterwards, by the filter paper filtering that extracting solution aperture is 20 μm, collect filtrate, repeat merging filtrate after said extracted process 6 times, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture II, the ethanol that concentration of volume percent is 95% is added to mixture II, after making mixing, the volumetric concentration of ethanol is 80%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely Scutellaria Barbata D. Don Polysaccharides is obtained by after filtrate evaporate to dryness,
The preparation of step 3, epimedium brevicornum polysaccharide
Herba Epimedii after pulverizing and distilled water are mixed and stir, the distilled water volume that every gram of Herba Epimedii is corresponding is 10ml, be the ultrasonic extraction 10min of 40kHz by frequency afterwards, by the filter paper filtering that extracting solution aperture is 20 μm, collect filtrate, repeat said extracted process 4-5 rear merging filtrate, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture III, the ethanol that volumetric concentration is 95% is added to mixture III, after making mixing, the volumetric concentration of ethanol is 70%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely epimedium brevicornum polysaccharide is obtained by after filtrate evaporate to dryness,
Step 4, preparation
Obtained Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide are mixed by the weight ratio of 1:1:1 and i.e. obtained vegetable polysaccharides after stirring.
Experiment one, the collocation of whole formula and preparation can be divided into following four steps
One, Herba Hedyotidis Diffusae polysaccharide preparation technology
It is as follows that Herba Hedyotidis Diffusae polysaccharide extracts preparation process, Spreading Hedyotis Herb is ground into powder, mixes with distilled water with certain solid-liquid ratio, carry out supersound extraction with the time set, and crosses leaching supernatant liquor, repeats to extract once, merge supernatant liquor.Rotary evaporation, to original volume 1/4th, adds ethanol, makes alcohol concn reach preset percentage, hold over night, and cross and filter insolubles, evaporate to dryness obtains polysaccharide.For optimizing polysaccharide yield and purity, it is as follows first to carry out experiment of single factor:
1, Herba Hedyotidis Diffusae polysaccharide extracts experiment of single factor
) test of different feed liquid ratio
Setting solid-liquid ratio (g/ml):
Material: Spreading Hedyotis Herb 5 g, distilled water
1:6，1:10，1:14，1:18，1:24。
Put into ultrasonication 30min, cross leaching supernatant liquor, repeat to extract once, merge supernatant liquor, rotary evaporation, to original volume 1/4th, adds ethanol, makes alcohol concn reach 60%, cross after spending the night and filter insolubles, evaporate to dryness obtains polysaccharide, and anthrone colorimetry measures polysaccharide.
B) test
Solid-liquid ratio 1:10(g/ml)
Material: Spreading Hedyotis Herb 5 g, distilled water
Setting puts into ultrasonic time 10min, 20min, 30min, 40min, 50min.
Cross leaching supernatant liquor, repeat to extract once, merge supernatant liquor, rotary evaporation, to original volume 1/4th, adds ethanol, makes alcohol concn reach 60%, and cross after spending the night and filter insolubles, evaporate to dryness obtains polysaccharide, and anthrone colorimetry measures polysaccharide.
) different extraction time
Solid-liquid ratio 1:10(g/ml)
Material: Spreading Hedyotis Herb 5 g, distilled water
Ultrasonic 30 min are put in setting, cross leaching supernatant liquor, and setting repeats extraction 1,2,3,4,5, secondary, merge supernatant liquor, rotary evaporation is to original volume 1/4th, add ethanol, make alcohol concn reach 60%, cross after spending the night and filter insolubles, evaporate to dryness obtains polysaccharide, and anthrone colorimetry measures polysaccharide.
D）
Solid-liquid ratio 1:10(g/ml)
Material: Spreading Hedyotis Herb 5 g, distilled water
Ultrasonic time 30 min is put in setting, crosses leaching supernatant liquor, repeats to extract once, merge supernatant liquor, rotary evaporation, to original volume 1/4th, adds ethanol, alcohol concn is made to reach 50%, 60%, 70%, 80%, 90%, cross after spending the night and filter insolubles, evaporate to dryness obtains polysaccharide, and anthrone colorimetry measures polysaccharide.
In experiment of single factor, each obtains the light absorption value (i.e. polysaccharide content) of sample
Determine thus, in orthogonal test, parameter solid-liquid ratio is 1:6,1:10 and 1:14; Ultrasonic time is 20min, 30min and 40min; Extraction time is 2,3 and 4 times; Alcohol concn is 60%, 70% and 90%.
2, Herba Hedyotidis Diffusae polysaccharide extracts orthogonal test
Orthogonal test parameter
Parameter designed in table is used to proceed as follows:
Solid-liquid ratio is the quality of Spreading Hedyotis Herb and the ratio of distilled water, and the quality of every part of Spreading Hedyotis Herb is 5g; The solution prepared in proportion (quality of every part of medicinal material is 5g) is put into ultrasonic wave; The ultrasonic time that will reach defined is stop 1 minute in every 5 minutes; Cross leaching supernatant liquor, repeat to extract, close rear also supernatant liquor; Rotary evaporation, to original volume 1/4th, adds the ethanol content of regulation, spends the night; Cross and filter insolubles, evaporate to dryness obtains polysaccharide; Anthrone colorimetry measures polysaccharide.
Orthogonal experiments is analyzed
Draw thus, the optimum extraction parameter of Herba Hedyotidis Diffusae polysaccharide is: solid-liquid ratio=1:14; Extraction time 30-40min; Repeat extraction time 3 times; Extract alcohol concn 60%.
Two, Scutellaria Barbata D. Don Polysaccharides preparation technology
It is as follows that Scutellaria Barbata D. Don Polysaccharides extracts preparation process, Herba Scutellariae Barbatae is ground into powder, mixes with distilled water with certain solid-liquid ratio, carry out supersound extraction with the time set, and crosses leaching supernatant liquor, repeats to extract once, merge supernatant liquor.Rotary evaporation, to original volume 1/4th, adds ethanol, makes alcohol concn reach preset percentage, hold over night, and cross and filter insolubles, evaporate to dryness obtains polysaccharide.For optimizing polysaccharide yield and purity, it is as follows first to carry out experiment of single factor:
1, Scutellaria Barbata D. Don Polysaccharides extracts experiment of single factor
A) different feed liquid is than test
Material: Herba Scutellariae Barbatae powder 5 g, distilled water
Setting solid-liquid ratio (g/ml): 1:6,1:10,1:14,1:18,1:24.
Put into ultrasonic time 30min, cross leaching supernatant liquor, repeat to extract once, merge supernatant liquor, rotary evaporation, to original volume 1/4th, adds ethanol, makes alcohol concn reach 60%, and cross after spending the night and filter insolubles, evaporate to dryness obtains polysaccharide, and anthrone colorimetry measures polysaccharide.
B) different supersound extraction time test
Solid-liquid ratio 1:10
Material: Herba Scutellariae Barbatae powder 5 g, distilled water
Setting puts into ultrasonic time 10min, 20min, 30min, 40min, 50min
Cross leaching supernatant liquor, repeat to extract once, merge supernatant liquor, rotary evaporation, to original volume 1/4th, adds ethanol, makes alcohol concn reach 60%, and cross after spending the night and filter insolubles, evaporate to dryness obtains polysaccharide, and anthrone colorimetry measures polysaccharide.
C) different extraction time
Solid-liquid ratio 1:10
Material: Herba Scutellariae Barbatae powder 5 g, distilled water
Ultrasonication 30 min is put in setting, crosses leaching supernatant liquor, and setting repeats extraction 1,2,3,4,5, secondary, merge supernatant liquor, rotary evaporation is to original volume 1/4th, add ethanol, make alcohol concn reach 60%, cross after spending the night and filter insolubles, evaporate to dryness obtains polysaccharide, and anthrone colorimetry measures polysaccharide.
D) different ethanol concentration
Solid-liquid ratio 1:10
Material: Herba Scutellariae Barbatae powder 5 g, distilled water
Ultrasonic time 30 min is put in setting, crosses leaching supernatant liquor, repeats to extract once, merge supernatant liquor, rotary evaporation, to original volume 1/4th, adds ethanol, alcohol concn is made to reach 50%, 60%, 70%, 80%, 90%, cross after spending the night and filter insolubles, evaporate to dryness obtains polysaccharide, and anthrone colorimetry measures polysaccharide.
In experiment of single factor, each obtains the light absorption value (i.e. polysaccharide content) of sample
Determine thus, in orthogonal test, parameter solid-liquid ratio is 1:6,1:10 and 1:14; Ultrasonic time is 10min, 20min and 30min; Extraction time is 3,5 and 6 times; Alcohol concn is 60%, 80% and 90%.
2, Scutellaria Barbata D. Don Polysaccharides extracts orthogonal test
Orthogonal test parameter
Parameter designed in table is used to proceed as follows:
Solid-liquid ratio be Herba Scutellariae Barbatae powder mass ratio on distilled water, the quality of every part of Herba Scutellariae Barbatae is 5g; The solution prepared in proportion (quality of every part of medicinal material is 5g) is put into ultrasonic wave; The ultrasonic time that will reach defined, every 5 minutes stop 1 minute; Cross leaching supernatant liquor, repeat to extract, merge supernatant liquor; Rotary evaporation, to original volume 1/4th, adds the ethanol content of regulation, spends the night; Cross and filter insolubles, evaporate to dryness obtains polysaccharide; Anthrone colorimetry measures polysaccharide.
Orthogonal experiments is analyzed
Draw thus, the optimum extraction parameter of Scutellaria Barbata D. Don Polysaccharides is solid-liquid ratio=1:14; Extraction time=10min; Repeat extraction time=5 time; Extract alcohol concn=80%.
Three, epimedium brevicornum polysaccharide preparation technology
It is as follows that epimedium brevicornum polysaccharide extracts preparation process, Herba Epimedii is ground into powder, mixes with distilled water with certain solid-liquid ratio, carry out supersound extraction with the time set, and crosses leaching supernatant liquor, repeats to extract once, merge supernatant liquor.Rotary evaporation, to original volume 1/4th, adds ethanol, makes alcohol concn reach preset percentage, hold over night, and cross and filter insolubles, evaporate to dryness obtains polysaccharide.For optimizing polysaccharide yield and purity, it is as follows first to carry out experiment of single factor:
1, epimedium brevicornum polysaccharide extracts experiment of single factor
A) different feed liquid is than test
Material: Epimedium Herb 5 g, distilled water
Setting solid-liquid ratio (g/ml) 1:6,1:10,1:14,1:18,1:24;
Put into ultrasonic time 30min; Cross leaching supernatant liquor, repeat to extract once, merge supernatant liquor; Rotary evaporation, to original volume 1/4th, adds ethanol, makes alcohol concn reach 60%, and cross after spending the night and filter insolubles, evaporate to dryness obtains polysaccharide, and anthrone colorimetry measures polysaccharide.
B) different supersound extraction time test
Solid-liquid ratio 1:10
Material: Epimedium Herb 5 g, distilled water
Setting puts into ultrasonic time 10min, 20min, 30min, 40min, 50min
Cross leaching supernatant liquor, repeat to extract once, merge supernatant liquor, rotary evaporation, to original volume 1/4th, adds ethanol, makes alcohol concn reach 60%, and cross after spending the night and filter insolubles, evaporate to dryness obtains polysaccharide, and anthrone colorimetry measures polysaccharide.
C) different extraction time
Solid-liquid ratio 1:10
Material: Epimedium Herb 5 g, distilled water
Ultrasonic 30 min are put in setting; Cross leaching supernatant liquor, setting repeats extraction 1,2,3,4,5 times, and merge supernatant liquor, rotary evaporation, to original volume 1/4th, adds ethanol, makes alcohol concn reach 60%, and cross after spending the night and filter insolubles, evaporate to dryness obtains polysaccharide, and anthrone colorimetry measures polysaccharide.
D) different ethanol concentration
Solid-liquid ratio 1:10
Material: Epimedium Herb 5 g, distilled water
Ultrasonic time 30 min is put in setting; Cross leaching supernatant liquor, repeat to extract once, merge supernatant liquor; Rotary evaporation, to original volume 1/4th, adds ethanol, makes alcohol concn reach 50%, 60%, 70%, 80%, 90%, and cross after spending the night and filter insolubles, evaporate to dryness obtains polysaccharide, and anthrone colorimetry measures polysaccharide.
In experiment of single factor, each obtains the light absorption value (i.e. polysaccharide content) of sample
Determine thus, in orthogonal test, parameter solid-liquid ratio is 1:6,1:10 and 1:22; Ultrasonic time is 10,30 and 50min; Extraction time is 2,5 and 6 times; Alcohol concn is 70%, 80% and 90%.
2, epimedium brevicornum polysaccharide extracts orthogonal test
Orthogonal test parameter
Parameter designed in table is used to proceed as follows:
Solid-liquid ratio be Epimedium Herb mass ratio on distilled water, the quality of every part of Herba Epimedii is 5g; The solution prepared in proportion (quality of every part of medicinal material is 5g) is put into ultrasonic wave; Ultrasonic wave will reach the time of defined, and every 5 minutes stop 1 minute; Cross leaching supernatant liquor, repeat to extract, close rear also supernatant liquor; Rotary evaporation, to original volume 1/4th, adds the ethanol content of regulation, spends the night; Cross and filter insolubles, evaporate to dryness obtains polysaccharide; Anthrone colorimetry measures polysaccharide.
Orthogonal experiments is analyzed
Draw thus, the optimum extraction parameter of epimedium brevicornum polysaccharide is: solid-liquid ratio=1:10; Extraction time=10min; Repeat extraction time=4 time; Extract alcohol concn=70%.
Four, Spreading Hedyotis Herb-Herba Scutellariae Barbatae-Herba Epimedii polysaccharide formula and preparation
For determining Spreading Hedyotis Herb, the optimum proportioning of Herba Scutellariae Barbatae and epimedium brevicornum polysaccharide, ad hoc meter nine assembly ratio, by three kinds of polysaccharide respectively with Spreading Hedyotis Herb: Herba Scutellariae Barbatae: Herba Epimedii=1:1:1; Spreading Hedyotis Herb: Herba Scutellariae Barbatae: Herba Epimedii=2:1:2; Spreading Hedyotis Herb: Herba Scutellariae Barbatae: Herba Epimedii=3:1:3; Spreading Hedyotis Herb: Herba Scutellariae Barbatae: Herba Epimedii=1:2:2; Spreading Hedyotis Herb: Herba Scutellariae Barbatae: Herba Epimedii=2:2:3; Spreading Hedyotis Herb: Herba Scutellariae Barbatae: Herba Epimedii=3:2:1; Spreading Hedyotis Herb: Herba Scutellariae Barbatae: Herba Epimedii=1:3:3; Spreading Hedyotis Herb: Herba Scutellariae Barbatae: Herba Epimedii=2:3:1; Spreading Hedyotis Herb: Herba Scutellariae Barbatae: Herba Epimedii=3:3:2 mixes.
To nine groups of samples the measuring DPPH free radical scavenging activity under different concns obtained, thus obtain its resistance of oxidation, result is as following table:
White in table: half: excessive, represent Herba Hedyotidis Diffusae polysaccharide: Scutellaria Barbata D. Don Polysaccharides: epimedium brevicornum polysaccharide.
Use orthogonal test to Spreading Hedyotis Herb in formula, the proportioning parameters relationship of Herba Scutellariae Barbatae and epimedium brevicornum polysaccharide is analyzed, and its result is as follows:
Orthogonal experiments is analyzed
Can be reached a conclusion, when Herba Hedyotidis Diffusae polysaccharide by above result: Scutellaria Barbata D. Don Polysaccharides: when epimedium brevicornum polysaccharide=1:1:1 carries out compatibility, formula can obtain best anti-oxidant activity.
Claims (2)
1. an anti-oxidant natural plant polyose, is characterized in that: described vegetable polysaccharides is made up of Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide, and the weight ratio preparing each component needed for vegetable polysaccharides is 1:1:1;
Described Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide are that Spreading Hedyotis Herb, Herba Scutellariae Barbatae and Herba Epimedii are obtained Spreading Hedyotis Herb extracting solution, Herba Scutellariae Barbatae extracting solution and extraction ofHerba Epimedii respectively by ultrasonication, then Spreading Hedyotis Herb extracting solution, Herba Scutellariae Barbatae extracting solution and the further evaporate to dryness of extraction ofHerba Epimedii are obtained respectively.
2. the preparation method of a kind of anti-oxidant natural plant polyose according to claim 1, is characterized in that, comprise the following steps:
The preparation of step one, Herba Hedyotidis Diffusae polysaccharide
Spreading Hedyotis Herb after pulverizing and distilled water are pressed the weight ratio mixing of 1:14, be the ultrasonic extraction 30-40min of 40kHz by frequency afterwards, extracting solution aperture is the filter paper filtering of 20 μm, collect filtrate, repeat said extracted process 2-3 time, merging filtrate, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture I, the ethanol that volumetric concentration is 95% is added to mixture I, after making mixing, the volumetric concentration of ethanol is 60%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely Herba Hedyotidis Diffusae polysaccharide is obtained by after filtrate evaporate to dryness,
The preparation of step 2, Scutellaria Barbata D. Don Polysaccharides
Herba Scutellariae Barbatae after pulverizing and distilled water are mixed and stir, the distilled water volume that every gram of Herba Scutellariae Barbatae is corresponding is 14ml, be the ultrasonic extraction 10min of 40kHz by frequency afterwards, by the filter paper filtering that extracting solution aperture is 20 μm, collect filtrate, repeat said extracted process 5-6 rear merging filtrate, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture II, the ethanol that concentration of volume percent is 95% is added to mixture II, after making mixing, the volumetric concentration of ethanol is 80%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely Scutellaria Barbata D. Don Polysaccharides is obtained by after filtrate evaporate to dryness,
The preparation of step 3, epimedium brevicornum polysaccharide
Herba Epimedii after pulverizing and distilled water are mixed and stir, the distilled water volume that every gram of Herba Epimedii is corresponding is 10ml, be the ultrasonic extraction 10min of 40kHz by frequency afterwards, by the filter paper filtering that extracting solution aperture is 20 μm, collect filtrate, repeat said extracted process 4-5 rear merging filtrate, and filtrate is put into Rotary Evaporators and be concentrated into original volume 1/4th, obtained mixture III, the ethanol that volumetric concentration is 95% is added to mixture III, after making mixing, the volumetric concentration of ethanol is 70%, after leaving standstill 24 h, with the filter paper filtering that aperture is 20 μm, namely epimedium brevicornum polysaccharide is obtained by after filtrate evaporate to dryness,
Step 4, preparation
Obtained Herba Hedyotidis Diffusae polysaccharide, Scutellaria Barbata D. Don Polysaccharides and epimedium brevicornum polysaccharide are mixed by the weight ratio of 1:1:1 and i.e. obtained vegetable polysaccharides after stirring.
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