CN103083352A - Application of oldenlandia diffusa extract to preparation of medicament for promoting growth and development of animals - Google Patents
Application of oldenlandia diffusa extract to preparation of medicament for promoting growth and development of animals Download PDFInfo
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- CN103083352A CN103083352A CN2013100277880A CN201310027788A CN103083352A CN 103083352 A CN103083352 A CN 103083352A CN 2013100277880 A CN2013100277880 A CN 2013100277880A CN 201310027788 A CN201310027788 A CN 201310027788A CN 103083352 A CN103083352 A CN 103083352A
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- herba hedyotidis
- hedyotidis diffusae
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Abstract
The invention discloses an application of oldenlandia diffusa extract to the preparation of a medicament for promoting the growth and the development of animals. The oldenlandia diffusa polysassharides are used as active components to prepare the medicament for promoting the growth and the development of animals. The oldenlandia diffusa polysassharides have the functions of increasing the growth rate of animals and can serve as novel animal growth promoters. Due to the adoption of the oldenlandia diffusa polysassharides, the daily gain of domestic animals can be increased, and the time of culturing domestic animals for sale can be shortened.
Description
Technical field
The invention belongs to veterinary drug, especially extract the new medicine use of the Herba Hedyotidis Diffusae polysaccharide that obtains from natural plants, be specifically related to their application in preparation promotion animal growth drug products.
Background technology
Along with scale and the intensivization development of animal husbandry, people more and more pay close attention to healthy aquaculture and food-safety problem.A large amount of uses of the chemical drugs take antibiotic as representative, though can play the effect of disease preventing and treating and promotion growth of animal, the problem of the bacterial drug resistance that it brings and livestock products veterinary drug residue is day by day serious.In this case, the natural drug take Chinese medicine as representative has shown huge advantage.
Herba Hedyotidis Diffusae (formal name used at school: Hedyotis diffusa) be the plant of Rubiaceae cerastium, begin to be stated from " Guangxi Chinese medicinal herbal ", its nature and flavor are bitter, sweet, cold, enter the heart, liver, spleen three warps, have clearing heat, releasing toxin, removing swelling, promoting blood circulation, removing blood stasis and relieving pain, effect that dampness removing is treating stranguria, for " one of medical material kind that the Chinese Pharmacopoeia 2005 version is recorded ([1] Chinese Pharmacopoeia Commission. Chinese Pharmacopoeia (an one) [S]. Beijing: chemical industry is published, and 2005: appendix 22.).much more the effect of Herba Hedyotidis Diffusae antitumor and raising immunity has report ([2] Qiaoyan Cai, Jiumao Lin, Lihui Wei, et al.Hedyotis diffusa Willd Inhibits Colorectal Cancer Growth in Vivo via Inhibition of STAT3Signaling Pathway[J] .International Journal of Molecular Sciences, 2012, 13, 6117-6128.[3] Meng Wei, Liu Zhiqiang, Qiu Shicui, Deng. the preliminary study [J] of Chinese medicine Herba Hedyotidis Diffusae on the immune function of mice impact. modern combination of Chinese and Western medicine magazine, 2005, 14 (2): 163-164.), wherein polysaccharide is one of main function composition of Herba Hedyotidis Diffusae, by rhamnose, glucose, galactose, arabinose and mannose form ([4] Bao Ludan, Xu Guofang, horse Zheng, Deng. pre-column derivatization HPLC is analyzed monosaccharide composition [J] in Herba Hedyotidis Diffusae polysaccharide. Chinese patent medicine, 2008, 30(3): 406-408.).Extract the toxicity that the separating polyose composition is conducive to reduce former medicine, improve curative effect, the production control quality.
in recent years, people have carried out tentatively probing into to Herba Hedyotidis Diffusae polysaccharide, mainly concentrate on the extraction purification, active research ([5] Shen Jinhui of the part such as antitumor and antioxidation, Yang Chunhua, Liu Jinghan. Herba Hedyotidis Diffusae polysaccharide Study on extraction [J]. Strait Pharmaceutical Journal, 2009, 21 (10): 83-85.[6] Qian Yunxu, Liu Pei, Li Li, Deng. the separation and purification of Herba Hedyotidis Diffusae polysaccharide and PMP column front derivation high-efficient liquid phase analysis [J]. Yunnan University of Traditional Chinese Medicine's journal, 2010, 33, (3): 43-46.[7] Lin, J, Chen, Y, Wei, et al.Hed yotis diffu-sa Willd extract induc-es apoptosis via activation of th e mitochondrion-dependent pathway in human colon carcinoma cells.In t.J.Oncol.2010, 37, 1331 – 1338.), but comparatively be short of in aspect technology such as its quality control and the application of feedstuff interpolation formulation development, there is not yet at present the research report that adopts the preparation of Herba Hedyotidis Diffusae polysaccharide extract to promote animal body growth promoter medicine both at home and abroad.
Summary of the invention
In view of the above deficiency of prior art, the objective of the invention is to study and make the Herba Hedyotidis Diffusae polysaccharide extract promote purposes in the animal growth medicine to make its being used widely in preparation animal growth medicine in preparation.
The present invention has studied the application of Herba Hedyotidis Diffusae polysaccharide in preparation promotion animal growth medicine, promotes the animal growth medicine take Herba Hedyotidis Diffusae polysaccharide as the active component manufacturing.
The invention provides the purposes of Herba Hedyotidis Diffusae extract, it has the effect purposes that improves animal growth rate, can be used as a kind of novel growth improver for livestock and poultry, improves the poultry daily gain, shortens marketing time.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Below zoopery be to further description of the present invention, but and do not mean that any limitation of the invention.Obviously, due to the selected laboratory animal pharmacology feasible popularity that has expressed the present invention use should trace back and the animal realm.
1, Herba Hedyotidis Diffusae extraction process
get Herba Hedyotidis Diffusae, be ground into coarse powder, join in extraction pot and clean up, inject the water of 12 times of amounts in the extraction pot, be heated to 100 ℃, after constant temperature 2.0 hours, emit medicinal liquid, the water that adds again 10 times of amounts is heated to 100 ℃, after constant temperature 1.5 hours, emit medicinal liquid, the water that adds again 8 times of amounts is heated to 100 ℃, after constant temperature 1.0 hours, merge medicinal liquid, filter, getting filtrate, to be concentrated into relative density be 1.10～1.20, after placing room temperature, adding 95% ethanol is 80% to containing the alcohol amount, the rear placement that stirs is spent the night, abandoning supernatant, precipitation is with appropriate 95% washing with alcohol 3 times, discard ethanol, residue is flung to ethanol, after adding a small amount of purified water dissolve complete, add 5% trifluoroacetic acid solution by the 1:1 volume, the rear placement that stirs is spent the night, get supernatant concentration to appropriate, dust, and get final product.
The Herba Hedyotidis Diffusae extract assay method
The preparation of standard solution is taken at 105 ℃ of anhydrous glucose 0.1g that are dried to constant weight, and precision takes, and puts in the 100ml volumetric flask, is dissolved in water, and is diluted to scale, shakes up, and get final product.
The preparation precision of need testing solution takes approximately 0.1mg of this product, puts in the 50ml measuring bottle, is dissolved in water and is diluted to scale, shakes up, and get final product.
The assay precision measures standard solution and each 2ml of need testing solution, puts respectively in tool plug test tube, respectively adds 3% phenol solution 2ml, shakes up, add rapidly sulphuric acid 9ml, shake up, be placed to room temperature, according to spectrophotography, the place measures respectively trap at the 490nm wavelength, calculates, and get final product.
The Herba Hedyotidis Diffusae extract polyoses content
This product is pressed dry product and is calculated, and every 1g contains Herba Hedyotidis Diffusae polysaccharide with glucose meter, must not be less than 450mg.
2, effect:
The Herba Hedyotidis Diffusae polysaccharide tool improves the effect of animal growth rate, can be used as a kind of novel growth improver for livestock and poultry, improves the poultry daily gain, shortens marketing time.
Adult mice with 50,100,150mg/kg body weight Herba Hedyotidis Diffusae polysaccharide gavage 7 days, can significantly put on weight; Broiler, piglet with 400,800, the 1200ppm spice fed 7 days continuously, can significantly improve body weight.
3, application example
(1) promote the growth of mice
Adult mice with 50,100,150mg/kg body weight Herba Hedyotidis Diffusae polysaccharide gavage 7 days, measured Mouse Weight in 1～28 day after administration, found that, Herba Hedyotidis Diffusae polysaccharide can significantly improve Mouse Weight.
After administration, different time is respectively organized the body weight (g) of mice
Annotate: a:P＜0.01, difference are extremely remarkable, b:P＜0.05, significant difference
(2) improve the body weight of broiler
7 Day-old Broiler Chickens with 400,800, the 1200ppm spice, administration 7 days, body weight was measured in 7 days, 14 days in the interval, found that Herba Hedyotidis Diffusae polysaccharide significantly increases broiler weight.
The body weight of different time broiler (g) after administration
Annotate: a:P＜0.01, difference are extremely remarkable, b:P＜0.05, significant difference
(3) reduce broiler material anharmonic ratio
Give continuous 7 Day-old Broiler Chickens of broiler with 400,800, the 1200ppm spice, administration 7 days, the material anharmonic ratio was measured at the interval in 7 days, 14 days, found that Herba Hedyotidis Diffusae polysaccharide significantly improves feed conversion rate, reduced and expected anharmonic ratio.
The material anharmonic ratio of different time broiler (feedstuff weight/body weight) after administration
(3) accelerate piglet growth
Give ablactational baby pig continuously with 400,800,1200ppm spice 7 days, the material anharmonic ratio was measured at the interval in 7 days, 14 days, found that Herba Hedyotidis Diffusae polysaccharide significantly improves feed conversion rate, reduced and expected anharmonic ratio.
35 days weight gain of piglets situations and material anharmonic ratio after administration
Claims (3)
1. the application of Herba Hedyotidis Diffusae extract in preparation promotion animal growth medicine, the medicine of promotion animal growth take Herba Hedyotidis Diffusae polysaccharide as the active component manufacturing.
2. according to claim 1 application is characterized in that, the medicine of described promotion animal growth promotes the growth of poultry or/and the purposes in reducing the medicine of broiler material anharmonic ratio in preparation.
3. described application according to claim 1 and 2, is characterized in that, Herba Hedyotidis Diffusae extract adopts following method to obtain:
Herba Hedyotidis Diffusae is ground into coarse powder, clean up, inject the water of 12 times of amounts, be heated to 100 ℃, after constant temperature 2.0 hours, emit medicinal liquid, the water that adds again 10 times of amounts is heated to 100 ℃, after constant temperature 1.5 hours, emit medicinal liquid, the water that adds again 8 times of amounts is heated to 100 ℃, after constant temperature 1.0 hours, merge medicinal liquid, filter, getting filtrate, to be concentrated into relative density be 1.10～1.20, after placing room temperature, adding 95% ethanol is 80% to containing the alcohol amount, the rear placement that stirs is spent the night, abandoning supernatant, precipitation is with appropriate 95% washing with alcohol 3 times, discard ethanol, residue is flung to ethanol, after adding a small amount of purified water dissolve complete, add 5% trifluoroacetic acid solution by the 1:1 volume, the rear placement that stirs is spent the night, get supernatant concentration to appropriate, dust, namely get object.
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| CN2013100277880A CN103083352A (en) | 2013-01-24 | 2013-01-24 | Application of oldenlandia diffusa extract to preparation of medicament for promoting growth and development of animals |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104059159A (en) * | 2014-06-17 | 2014-09-24 | 湖南农业大学 | Cellulase-catalyzed method for extracting spreading hedyotis herb polysaccharides |
| CN104292353A (en) * | 2014-09-30 | 2015-01-21 | 河南科技大学 | Anti-oxidation natural plant polysaccharide and preparation method thereof |
-
2013
- 2013-01-24 CN CN2013100277880A patent/CN103083352A/en active Pending
Non-Patent Citations (3)
| Title |
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| 《广东畜牧兽医科技》 20061231 王刚 "壮能"饲料增效剂对猪生长性能及肉质的影响 22-24 1-3 第31卷, 第4期 * |
| 王刚: ""壮能"饲料增效剂对猪生长性能及肉质的影响", 《广东畜牧兽医科技》 * |
| 钱韵旭等: "白花蛇舌草多糖的分离纯化及PMP柱前衍生高效液相分析", 《云南中医学院学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104059159A (en) * | 2014-06-17 | 2014-09-24 | 湖南农业大学 | Cellulase-catalyzed method for extracting spreading hedyotis herb polysaccharides |
| CN104059159B (en) * | 2014-06-17 | 2016-04-20 | 湖南农业大学 | The processing method of Mierocrystalline cellulose Enzymatic Extraction Herba Hedyotidis Diffusae polysaccharide |
| CN104292353A (en) * | 2014-09-30 | 2015-01-21 | 河南科技大学 | Anti-oxidation natural plant polysaccharide and preparation method thereof |
| CN104292353B (en) * | 2014-09-30 | 2016-06-29 | 河南科技大学 | A kind of antioxidation natural plant polyose and preparation method thereof |
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Application publication date: 20130508 |