CN104286238A - Technology for carrying out fungus growing on loose tea - Google Patents
Technology for carrying out fungus growing on loose tea Download PDFInfo
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- CN104286238A CN104286238A CN201410482495.6A CN201410482495A CN104286238A CN 104286238 A CN104286238 A CN 104286238A CN 201410482495 A CN201410482495 A CN 201410482495A CN 104286238 A CN104286238 A CN 104286238A
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Abstract
The invention discloses a technology for carrying out fungus growing on loose tea. The technology comprises the following steps: (1) preparing a fermenting agent, namely, separating out eurotium cristatum from fuzhuan tea, and preparing to obtain the solid pure strain fermenting agent; (2) treating raw dark green tea raw material, namely, removing the impurities in the raw dark green tea, steaming, carrying out pile fermentation and then feeding tea juice; (3) carrying out steaming inoculation and fungus growing, namely, steaming the raw dark green tea with the tea juice, then immediately feeding the solid pure strain fermenting agent and inoculating; after that, putting the inoculated raw dark green tea into a fungus growing culture room, and carrying out fungus growing culture; and (4) drying and packaging, namely, drying after fungus growing, and directly packaging the dried loose tea. After the technology is adopted, the fungus growing time is shortened, the bacterial pollution rate can be reduced in the fungus growing process of the loose tea, the problems that the existing loose tea is hard in fungus growing and easy in bacterial growth can be solved, the fungus growing rate of the loose tea reaches up to 100%, and the tea is widespread in golden flowers on the surface and the granules are plump.
Description
Technical field
The present invention relates to processing field of tea leaves, be specifically related to a kind of loose tea fungus growing process.
Background technology
Fu-brick tea is most characteristic black tea product, " growing dim " is Fu-brick tea characteristic mark, also be the key technology that it is produced, " golden flower bacterium " (the formal name used at school coronoid process fall apart capsule bacterium) formed by fermentation, under the microscope in golden yellow, Form Sphere, it can grow under suitable temperature and humidity condition in compressed tea, secrete a series of enzyme, impel Substance Transformation in tealeaves, thus form many functional components useful to human body.Research shows " golden flower " in Fu tea and lucidum spore powder very roughly the same, effectively can regulate human metabolism, and have stronger lipopenicillinase, step-down, adjustment carbohydate metabolism, promote the effect such as body immunity, people golden flower in tealeaves number depending on doing the quality quality of Fu-brick tea.
But Fu-brick tea complex manufacturing technology, the production cycle is long, and conditional request of growing dim higher and product quality difficulty controls, and is difficult to form scale pile line operation, needs tea block to pry open, be not easy to popular consumption when particularly drinking.At present for the research of the technology of growing dim mainly in compressed tea, less and immature to loose tea technical research of growing dim, because golden flower bacterium requires strict at loose tealeaves surface developmental condition, and easy pollution microbes, cause loose tea technical difficulty of growing dim large.Application number be 201010598870.5 denomination of invention be the patent of " Fu-brick tea grow dim loose tea preparation technology ", " golden flower bacterium " is made spore suspension, and its technique comprises the fall apart separation and purification of capsule bacterium, the preparation of eurotium cristatum spore suspension and concentration of coronoid process and determines (10
7~ 10
8individual/mL), Dark Green Tea process, add eurotium cristatum spore suspension, tea curing, control humiture are grown dim, loose tea dry and preserve.But long good coronoid process fall apart capsule bacterium PDA flat board on prepare spore suspension, the amount of the spore suspension that can prepare is limited, large to the demand of spore suspension when loose tea grows dim, and is not suitable for the demand of production practices.The denomination of invention of application number 201210148773.1 is the patent of " a kind of golden-flower loose tea manufacture craft of improvement ", and the good tea grinding that will grow dim is broken, is inoculated on tealeaves by the weight ratio of 1% ~ 2%.Its technique is: raw material choice, gas steam wet heap, moisture adjustment, golden flower inoculation, mounted box, high temperature steaming, grow dim, dry.But due in tea powder containing multiple miscellaneous bacteria, uncontrollable loose tea grows dim, and carries out high temperature and high pressure steam process after inoculation, and it is active to destroy the golden flower bacterium joined in tealeaves, cannot ensure that coronoid process falls apart the growth vigor of capsule bacterium, also just cannot produce golden flower.
Summary of the invention
In order to overcome the problems referred to above, the object of the present invention is to provide a kind of loose tea fungus growing process, this technique produces the golden flower bacterium of full grains while shortening the cycle of growing dim, and this technology is applicable to scale pile line operation.
For achieving the above object, the present invention adopts following technical scheme:
A kind of loose tea fungus growing process, comprises the following steps:
1) preparation of leavening: first isolate coronoid process and fall apart capsule bacterium from Fu-brick tea, is purified low temperature slant preservation after qualification, uses PDA to spread cultivation for subsequent use; Then starter medium is prepared; The capsule bacterium that fallen apart by the coronoid process spread cultivation is mixed with bacterium liquid and is inoculated on culture medium, obtains coronoid process and fall apart capsule bacterium culture after cultivation; The capsule bacterium culture that finally fallen apart by coronoid process is dry, and grind after drying and sieve, fall apart the coronoid process sieved capsule bacterium culture and KH
2pO
4, MgSO
4solid pure culture leavening is obtained according to the mass ratio preparation of 7:2:1;
2) process of Dark Green Tea raw material: by Dark Green Tea impurity elimination, then steams through gas and adds tea juice after wet heap;
3) gas steams inoculation and grows dim: the Dark Green Tea adding tea juice is carried out gas steaming, and gas adds solid pure culture leavening immediately and inoculates after steaming, and then the Dark Green Tea to take over kind is loaded in the gas-permeable container that sterilization crosses; And be placed in culturing room of growing dim and carry out cultivation of growing dim;
4) dry: to take out gas-permeable container after growing dim and drying.
Further, step 1) described in starter medium comprise the tea that tea-water proportion is 1/40, every 100L tea adds wheat bran 100kg, glucose 6kg, yeast leaching powder 4kg, NaCl 0.8kg.
Further, the temperature of described starter medium at 121 DEG C or the sterilized under pressure 30min of 0.1 ~ 0.15MPa.
Further, step 1) in the coronoid process that spreads cultivation fall apart that to be mixed with concentration be 2 × 10 to capsule bacterium
7~ 9 × 10
7the bacterium liquid of individual/ml, inoculum concentration is 20ml/kg relative to culture medium.
Further, step 1) in the temperature regulation mechanism of incubation be: incubation time is 8 days, and first three sky is 27 ~ 29 DEG C, and latter five days is 25 ~ 28 DEG C; The coronoid process obtained after the cultivation bacterium number in capsule bacterium culture that falls apart reaches 3 × 10
9~ 8 × 10
9/ g.
Further, step 1) in drying be adopt vacuum drying mode under 100 grades of purification conditions, baking temperature is 35 ~ 41 DEG C; Milled 60 mesh sieve after dry.
Further, step 2) in gas steam condition be: under the steam of 116 ~ 121 DEG C gas steam 3 ~ 5min; Wet heap condition is: wet heap 6h at 45 ~ 55 DEG C; Adding tea juice regulates Dark Green Tea water content to be 25 ~ 27%.
Further, step 3) in gas condition of steaming be: the Dark Green Tea adding tea juice is put into gas steamer, utilizes the steam gas of 0.25Mpa to steam 15 ~ 20s; Solid pure culture leavening adds by 0.2% of the Dark Green Tea weight adding tea juice.
Further, step 3) in gas-permeable container in layering discharge in culturing room of growing dim, upward, cultivation temperature setting degree is 26 ~ 28 DEG C to ventilation mouth, humidity 60 ~ 70%; From there is yellow particle in container, temperature is set to 28 ~ 30 DEG C, and is opened completely by gas-permeable container to strengthen aeration condition.
Further, step 4) in baking temperature be 40 ~ 45 DEG C, the water content controlling dried loose tea is 8 ~ 10%.
Compared with prior art, the present invention has following useful technique effect:
Golden flower bacterium distinctive in Fu-brick tea is prepared into solid fermentation agent by the present invention, successfully golden flower bacterium is incorporated in the middle of loose tea with the form of leavening, the time of growing dim can not only be shortened, and the bacterial contamination rate that loose tea grows dim in process can be reduced, solve current loose tea grow dim difficulty, easily grow the problem of miscellaneous bacteria; Meanwhile, this leavening is not only applicable to loose tea and grows dim, and also can be used for other tealeaves as compressed tea etc., and this leavening viable count is high, effect is many, consumption is few, be convenient to packaging, is applicable to suitability for industrialized production, can reduces entreprise cost; Adopt gas-permeable container to carry out temperature and humidity control to grow dim and shorten the cycle of growing dim, improve production efficiency, energy-saving and cost-reducing, improve drinking safety and the convenience of product.Result of the present invention shows, the loose tea rate of growing dim reaches 100%, golden flower is general on tealeaves surface, millet paste given off a strong fragrance arohid flavour, flavour alcohol and, soup look orange red, the loose tea that the method obtains conveniently is drunk, and has effect same with Fu-brick tea, decreases Fu-brick tea and must prize the trouble of drinking, give up consuming misgivings, adapted to current fast pace life requirement.
Detailed description of the invention
Embodiments of the present invention are:
A kind of loose tea fungus growing process, comprises the following steps:
1) preparation of leavening:
1. from Fu-brick tea, first isolate coronoid process to fall apart capsule bacterium, be purified low temperature slant preservation after qualification, use PDA to spread cultivation for subsequent use;
2. then prepare starter medium, starter medium comprises the tea that tea-water proportion is 1/40, and every 100L tea adds wheat bran 100kg, glucose 6kg, yeast leaching powder 4kg, NaCl 0.8kg; Described starter medium is at the temperature of 121 DEG C or the sterilized under pressure 30min of 0.1 ~ 0.15MPa;
3. it is 2 × 10 that the capsule bacterium that fallen apart by the coronoid process spread cultivation is mixed with concentration
7~ 9 × 10
7the bacterium liquid of individual/mL, be inoculated on above-mentioned culture medium, inoculum concentration is 20ml/kg relative to above-mentioned culture medium, incubation time 8 days, first three sky of cultivation temperature is 27 ~ 29 DEG C, and latter five days is 25 ~ 28 DEG C, when golden yellow cleistothecium covers media surface completely, loosen charge level and stir, impelling and evenly fully grow dim, reach bacterium number 3 × 10
9~ 8 × 10
9/ g, obtains coronoid process and to fall apart capsule bacterium culture;
4. the last capsule bacterium culture that fallen apart by coronoid process adopts vacuum drying mode dry with the temperature of 35 ~ 41 DEG C under 100 grades of purification conditions, and milled 60 mesh sieves after dry, fall apart the coronoid process sieved capsule bacterium culture and KH
2pO
4and MgSO
4solid pure culture leavening is obtained according to the mass ratio preparation of 7:2:1;
2) process of Dark Green Tea raw material: by Dark Green Tea impurity elimination, then under the steam of 116 ~ 121 DEG C gas steam 3 ~ 5min, then at 45 ~ 55 DEG C wet heap 6h, material in tealeaves is transformed, after wet heap, add tea juice and regulate water content of tea to be 25 ~ 27%, be beneficial to coronoid process and fall apart capsule bacteria growing;
3) gas steams inoculation and grows dim: the Dark Green Tea adding tea juice is put into gas steamer, utilizes the steam gas of 0.25Mpa to steam 15 ~ 20s; Gas adds rapidly solid pure culture leavening after steaming and mixes, solid pure culture leavening adds by 0.2% of the Dark Green Tea weight adding tea juice, then the Dark Green Tea to take over kind is loaded in the gas-permeable container that sterilization crosses, by gas-permeable container in layering discharge in culturing room of growing dim, ventilation mouth upward, cultivation temperature setting degree is 26 ~ 28 DEG C, humidity 60 ~ 70%; From there is yellow particle in container, temperature is set to 28 ~ 30 DEG C, and strengthens aeration condition;
4) dry and packaging: take out gas-permeable container after growing dim dry at 40 ~ 45 DEG C, the water content controlling dried loose tea is 8 ~ 10%, and dried loose tea is directly packed.
The present invention can also implement in the following manner:
Step 1) with step 2) identical with said method;
3) secondary gas steams, and after wet heap, adds tea juice and regulates water content of tea to be 25%, under pressure 0.1Mpa, temperature 121 DEG C of conditions, carry out the steaming of secondary gas, to kill the miscellaneous bacteria in tealeaves;
4) set length × wide × floor height as the support of 3m × 1.5m × .03m in the bacterium indoor of sending out of cleaning, frame is put several 1m × 0.5m × .0.08m charging tray, be laid in sterile charging tray by sterilized tealeaves while hot, thickness is 2cm ~ 3cm, charge level covers two-layer sterilized wet gauze;
5) when tealeaves is cooled to below 30 DEG C by sterile working requirement, be sprinkled in tealeaves by leavening, and stir with sterile glass rod, solid pure culture leavening addition is 0.3% of dry weight of tea leaves;
6) strict humiture and the ventilation condition controlling desinfection chamber, first 3 days temperature of fermenting are 27 ~ 29 DEG C, from tealeaves, humidity 60 ~ 70%, occurs that after yellow particle, wet down heats, and are beneficial to maintain coronoid process and fall apart the growth vigor of capsule bacterium;
7) after growing dim 8 days, progressively rise to 40 ~ 55 DEG C in room temperature of growing dim and dehydrate, control the water content of loose tea 8 ~ 10%, directly pack after dry.
Below in conjunction with embodiment, the present invention is described in further detail:
Embodiment 1
A kind of loose tea fungus growing process, comprises the following steps:
1) preparation of leavening:
1. from Fu-brick tea, first isolate coronoid process to fall apart capsule bacterium, be purified low temperature slant preservation after qualification, use PDA to spread cultivation for subsequent use;
2. then prepare starter medium, starter medium comprises the tea that tea-water proportion is 1/40, and every 100L tea adds wheat bran 100kg, glucose 6kg, yeast leaching powder 4kg, NaCl 0.8kg; Described starter medium is at the temperature of 121 DEG C or the sterilized under pressure 30min of 0.1MPa;
3. it is 2 × 10 that the capsule bacterium that fallen apart by the coronoid process spread cultivation is mixed with concentration
7~ 9 × 10
7the bacterium liquid of individual/mL, be inoculated on above-mentioned culture medium, inoculum concentration is 20ml/kg relative to above-mentioned culture medium, incubation time 8 days, first three sky of cultivation temperature is 27 DEG C, and latter five days is 25 DEG C, when golden yellow cleistothecium covers media surface completely, loosen charge level and stir, impelling and evenly fully grow dim, reach bacterium number 3 × 10
9~ 8 × 10
9/ g, obtains coronoid process and to fall apart capsule bacterium culture;
4. the last capsule bacterium culture that fallen apart by coronoid process adopts vacuum drying mode dry with the temperature of 35 DEG C under 100 grades of purification conditions, and milled 60 mesh sieves after dry, fall apart the coronoid process sieved capsule bacterium culture and KH
2pO
4and MgSO
4solid pure culture leavening is obtained according to the mass ratio preparation of 7:2:1;
2) process of Dark Green Tea raw material: by Dark Green Tea impurity elimination, then under the steam of 116 DEG C gas steam 5min, then at 45 DEG C wet heap 6h, material in tealeaves is transformed, after wet heap, add tea juice and regulate water content of tea to be 25%, be beneficial to coronoid process and fall apart capsule bacteria growing;
3) gas steams inoculation and grows dim: the Dark Green Tea adding tea juice is put into gas steamer, utilizes the steam gas of 0.25Mpa to steam 15s; Gas adds rapidly solid pure culture leavening after steaming and mixes, solid pure culture leavening adds by 0.2% of the Dark Green Tea weight adding tea juice, then the Dark Green Tea to take over kind is loaded in the polypropylene venting bags that sterilization crosses or in crisper, tealeaves height is about 2/3 of container height, and tighten sack with the collar and upper shield, the tea bag installed or caddy are discharged in layering in culturing room of growing dim, ventilation mouth is upward, cultivation temperature setting degree is 26 DEG C, humidity 60%; From there is yellow particle in container, temperature is set to 28 DEG C, and tea bag or caddy is opened to strengthen aeration condition completely;
4) dry and packaging: take out gas-permeable container after growing dim dry at 40 ~ 45 DEG C, the water content controlling dried loose tea is 8 ~ 10%, and dried loose tea is directly packed.
Embodiment 2
A kind of loose tea fungus growing process, comprises the following steps:
1) preparation of leavening:
1. from Fu-brick tea, first isolate coronoid process to fall apart capsule bacterium, be purified low temperature slant preservation after qualification, use PDA to spread cultivation for subsequent use;
2. then prepare starter medium, starter medium comprises the tea that tea-water proportion is 1/40, and every 100L tea adds wheat bran 100kg, glucose 6kg, yeast leaching powder 4kg, NaCl 0.8kg; Described starter medium is at the temperature of 121 DEG C or the sterilized under pressure 30min of 0.12MPa;
3. it is 2 × 10 that the capsule bacterium that fallen apart by the coronoid process spread cultivation is mixed with concentration
7~ 9 × 10
7the bacterium liquid of individual/mL, be inoculated on above-mentioned culture medium, inoculum concentration is 20ml/kg relative to above-mentioned culture medium, incubation time 8 days, first three sky of cultivation temperature is 28 DEG C, and latter five days is 26 DEG C, when golden yellow cleistothecium covers media surface completely, loosen charge level and stir, impelling and evenly fully grow dim, reach bacterium number 3 × 10
9~ 8 × 10
9/ g, obtains coronoid process and to fall apart capsule bacterium culture;
4. the last capsule bacterium culture that fallen apart by coronoid process adopts vacuum drying mode dry with the temperature of 38 DEG C under 100 grades of purification conditions, and milled 60 mesh sieves after dry, fall apart the coronoid process sieved capsule bacterium culture and KH
2pO
4and MgSO
4solid pure culture leavening is obtained according to the mass ratio preparation of 7:2:1;
2) process of Dark Green Tea raw material: by Dark Green Tea impurity elimination, then under the steam of 118 DEG C gas steam 4min, then at 50 DEG C wet heap 6h, material in tealeaves is transformed, after wet heap, add tea juice and regulate water content of tea to be 26%, be beneficial to coronoid process and fall apart capsule bacteria growing;
3) gas steams inoculation and grows dim: the Dark Green Tea adding tea juice is put into gas steamer, utilizes the steam gas of 0.25Mpa to steam 17s; Gas adds rapidly solid pure culture leavening after steaming and mixes, solid pure culture leavening adds by 0.2% of the Dark Green Tea weight adding tea juice, then the Dark Green Tea to take over kind is loaded in the polypropylene venting bags that sterilization crosses or in crisper, tealeaves height is about 2/3 of container height, and tighten sack with the collar and upper shield, the tea bag installed or caddy are discharged in layering in culturing room of growing dim, ventilation mouth is upward, cultivation temperature setting degree is 27 DEG C, humidity 65%; From there is yellow particle in container, temperature is set to 29 DEG C, and tea bag or caddy is opened to strengthen aeration condition completely;
4) dry and packaging: take out gas-permeable container after growing dim dry at 40 ~ 45 DEG C, the water content controlling dried loose tea is 8 ~ 10%, and dried loose tea is directly packed.
Embodiment 3
A kind of loose tea fungus growing process, comprises the following steps:
1) preparation of leavening:
1. from Fu-brick tea, first isolate coronoid process to fall apart capsule bacterium, be purified low temperature slant preservation after qualification, use PDA to spread cultivation for subsequent use;
2. then prepare starter medium, starter medium comprises the tea that tea-water proportion is 1/40, and every 100L tea adds wheat bran 100kg, glucose 6kg, yeast leaching powder 4kg, NaCl 0.8kg; Described starter medium is at the temperature of 121 DEG C or the sterilized under pressure 30min of 0.15MPa;
3. it is 2 × 10 that the capsule bacterium that fallen apart by the coronoid process spread cultivation is mixed with concentration
7~ 9 × 10
7the bacterium liquid of individual/mL, be inoculated on above-mentioned culture medium, inoculum concentration is 20ml/kg relative to above-mentioned culture medium, incubation time 8 days, first three sky of cultivation temperature is 29 DEG C, and latter five days is 28 DEG C, when golden yellow cleistothecium covers media surface completely, loosen charge level and stir, impelling and evenly fully grow dim, reach bacterium number 3 × 10
9~ 8 × 10
9/ g, obtains coronoid process and to fall apart capsule bacterium culture;
4. the last capsule bacterium culture that fallen apart by coronoid process adopts vacuum drying mode dry with the temperature of 41 DEG C under 100 grades of purification conditions, and milled 60 mesh sieves after dry, fall apart the coronoid process sieved capsule bacterium culture and KH
2pO
4and MgSO
4solid pure culture leavening is obtained according to the mass ratio preparation of 7:2:1;
2) process of Dark Green Tea raw material: by Dark Green Tea impurity elimination, then under the steam of 121 DEG C gas steam 3min, then at 55 DEG C wet heap 6h, material in tealeaves is transformed, after wet heap, add tea juice and regulate water content of tea to be 27%, be beneficial to coronoid process and fall apart capsule bacteria growing;
3) gas steams inoculation and grows dim: the Dark Green Tea adding tea juice is put into gas steamer, utilizes the steam gas of 0.25Mpa to steam 20s; Gas adds rapidly solid pure culture leavening after steaming and mixes, solid pure culture leavening adds by 0.2% of the Dark Green Tea weight adding tea juice, then the Dark Green Tea to take over kind is loaded in the polypropylene venting bags that sterilization crosses or in crisper, tealeaves height is about 2/3 of container height, and tighten sack with the collar and upper shield, the tea bag installed or caddy are discharged in layering in culturing room of growing dim, ventilation mouth is upward, cultivation temperature setting degree is 28 DEG C, humidity 70%; From there is yellow particle in container, temperature is set to 30 DEG C, and tea bag or caddy is opened to strengthen aeration condition completely;
4) dry and packaging: take out gas-permeable container after growing dim dry at 40 ~ 45 DEG C, the water content controlling dried loose tea is 8 ~ 10%, and dried loose tea is directly packed.
Golden flower bacterium distinctive in Fu-brick tea is prepared into solid fermentation agent by the present invention, successfully golden flower bacterium is incorporated in the middle of loose tea with the form of leavening, the time of growing dim can not only be shortened, and the bacterial contamination rate that loose tea grows dim in process can be reduced, solve current loose tea grow dim difficulty, easily grow the problem of miscellaneous bacteria;
Result shows: the loose tea rate of growing dim obtained by the method for the present invention reaches 100%, golden flower is general on tealeaves surface, full grains, millet paste given off a strong fragrance arohid flavour, flavour alcohol and, soup look orange red, the loose tea that the method obtains conveniently is drunk, and has effect same with Fu-brick tea, decreases Fu-brick tea and must prize the trouble of drinking, give up consuming misgivings, adapted to current fast pace life requirement.
Claims (10)
1. a loose tea fungus growing process, is characterized in that, comprises the following steps:
1) preparation of leavening: first isolate coronoid process and fall apart capsule bacterium from Fu-brick tea, is purified low temperature slant preservation after qualification, uses PDA to spread cultivation for subsequent use; Then starter medium is prepared; The capsule bacterium that fallen apart by the coronoid process spread cultivation is mixed with bacterium liquid and is inoculated on culture medium, obtains coronoid process and fall apart capsule bacterium culture after cultivation; The capsule bacterium culture that finally fallen apart by coronoid process is dry, and grind after drying and sieve, fall apart the coronoid process sieved capsule bacterium culture and KH
2pO
4, MgSO
4solid pure culture leavening is obtained according to the mass ratio preparation of 7:2:1;
2) process of Dark Green Tea raw material: by Dark Green Tea impurity elimination, then steams through gas and adds tea juice after wet heap;
3) gas steams inoculation and grows dim: the Dark Green Tea adding tea juice is carried out gas steaming, and gas adds solid pure culture leavening immediately and inoculates after steaming, and then the Dark Green Tea to take over kind is loaded in the gas-permeable container that sterilization crosses; And be placed in culturing room of growing dim and carry out cultivation of growing dim;
4) dry: to take out gas-permeable container after growing dim and drying.
2. a kind of loose tea fungus growing process according to claim 1, is characterized in that, step 1) described in starter medium comprise the tea that tea-water proportion is 1/40, every 100L tea adds wheat bran 100kg, glucose 6kg, yeast leaching powder 4kg, NaCl 0.8kg.
3. a kind of loose tea fungus growing process according to claim 1, is characterized in that, the temperature of described starter medium at 121 DEG C or the sterilized under pressure 30min of 0.1 ~ 0.15MPa.
4. a kind of loose tea fungus growing process according to claim 1, is characterized in that, step 1) in the coronoid process that spreads cultivation fall apart that to be mixed with concentration be 2 × 10 to capsule bacterium
7~ 9 × 10
7the bacterium liquid of individual/ml, inoculum concentration is 20ml/kg relative to culture medium.
5. a kind of loose tea fungus growing process according to claim 1, is characterized in that, step 1) in the temperature regulation mechanism of incubation be: incubation time is 8 days, and first three sky is 27 ~ 29 DEG C, and latter five days is 25 ~ 28 DEG C; The coronoid process obtained after the cultivation bacterium number in capsule bacterium culture that falls apart reaches 3 × 10
9~ 8 × 10
9/ g.
6. a kind of loose tea fungus growing process according to claim 1, is characterized in that, step 1) in drying be adopt vacuum drying mode under 100 grades of purification conditions, baking temperature is 35 ~ 41 DEG C; Milled 60 mesh sieve after dry.
7. a kind of loose tea fungus growing process according to claim 1, is characterized in that, step 2) in gas steam condition be: under the steam of 116 ~ 121 DEG C gas steam 3 ~ 5min; Wet heap condition is: wet heap 6h at 45 ~ 55 DEG C; Adding tea juice regulates Dark Green Tea water content to be 25 ~ 27%.
8. a kind of loose tea fungus growing process according to claim 1, is characterized in that, step 3) in gas steam condition be: the Dark Green Tea adding tea juice is put into gas steamer, utilize 0.25Mpa steam gas steam 15 ~ 20s; Solid pure culture leavening adds by 0.2% of the Dark Green Tea weight adding tea juice.
9. a kind of loose tea fungus growing process according to claim 1, is characterized in that, step 3) in gas-permeable container in layering discharge of growing dim in culturing room, upward, cultivation temperature setting degree is 26 ~ 28 DEG C to ventilation mouth, humidity 60 ~ 70%; From there is yellow particle in container, temperature is set to 28 ~ 30 DEG C, and is opened completely by gas-permeable container to strengthen aeration condition.
10. a kind of loose tea fungus growing process according to claim 1, is characterized in that, step 4) in baking temperature be 40 ~ 45 DEG C, the water content controlling dried loose tea is 8 ~ 10%.
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| CN105230827A (en) * | 2015-10-13 | 2016-01-13 | 杭州艺福堂茶业有限公司 | Eurotium cristatum tea automatic fermentation process |
| CN105901212A (en) * | 2016-04-15 | 2016-08-31 | 四川师范大学 | Docynia delavayi (Franch.)Schneid. fermented tea and preparation method thereof |
| CN106417724A (en) * | 2016-06-24 | 2017-02-22 | 中国土产畜产进出口总公司 | Loose tea Eurotium cristatum growth technology |
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