CN103275876A - Tea source eurotium cristatum strain and application thereof - Google Patents
Tea source eurotium cristatum strain and application thereof Download PDFInfo
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- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
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- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Tea And Coffee (AREA)
Abstract
The invention discloses a tea source eurotium cristatum strain which is named as eurotium cristatum XU08 and is preserved in the CGMCC (China General Microbiological Culture Collection Center) on March 13, 2012 with a preservation number of CGMCC No.5891. Researches on the morphological feature, the physiological property, the genetic stability and the growing conditions of the strain during the production of the Fuzhuan tea and industrial trial production of the strain prove that the strain is available as a special leavening agent for the production of the Fuzhuan tea, so that the production cycle can be shortened and the product quality can be improved, and therefore, the strain is very suitable for modern production needs of the Fuzhuan tea.
Description
Technical field
The invention belongs to microorganism and use and the food processing technology field, be specifically related to the diffusing capsule bacteria strain of a strain tea source coronoid process and the application in Fu brick tea production fermentation thereof.
Technical background
The Fu brick tea belongs to fully fermenting tea, is that complete processing is the most complicated in all teas, unique black tea product.Be commonly called as the golden yellow point of " golden flower " at the tealeaves of Fu brick tea finished product surface ubiquity, this is the key character that the Fu brick tea is different from other brick tea." golden flower " is the golden yellow cleistothecium that the diffusing capsule bacterium of the coronoid process in the Fu brick tea produces, and how much " golden flower's " is the important indicator of Fu brick tea quality quality.
The diffusing capsule bacterium of coronoid process can produce various extracellular enzymes (as polyphenoloxidase, polygalacturonase, cellulase, proteolytic enzyme etc.), as effective biochemical power, various related substances generation oxidations, polymerization, degraded, conversion in the catalysis tealeaves improve the quality of Fu brick tea, form the distinctive local flavor of Fu brick tea.Spontaneous fermentation is produced in the Fu brick tea process, and the production cycle is generally longer, and earlier fermentation is easy to generate multiple assorted bacterium, causes Fu brick tea quality instability, can cause the Fu brick tea to go mouldy when serious and scrap.
Summary of the invention
Technical problem to be solved by this invention is: at above-mentioned the deficiencies in the prior art, provide a kind of coronoid process capsule bacterium (Eurotium cristatum) XU08 that looses, use when can be used as Fu brick tea production fermentation.
Coronoid process of the present invention capsule bacterium (Eurotium cristatum) XU08 that looses, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on March 13rd, 2012, deposit number is: CGMCC No.5891, preservation address: No. 3 Institute of Microorganism, Academia Sinica in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
The character that strain X U08 of the present invention has is as follows:
On the M40Y solid medium, cultivated 7 days for 25 ℃, can form diameter greater than the bacterium colony of 85 mm, and also can grow at 38 ℃.The bacterium colony periphery has a circle white to light yellow mycelium, and it is light yellow that inside is bordering on olive, and the middle body color is darker, is bordering on brown, after 14 days middle body become brown, again in outwards color shoal gradually, the surface forms closes yellow softgel shell in a large number.The dark brown yellow of bacterium colony reverse side, pigment diffuses in the matrix.With the visible cleistothecium surface irregularity of microscopic examination, obvious coronal process is arranged, the thecaspore of the little wart of tool and spinulate conidium; Cleistothecium is shaped as sphere or almost spherical by tool decorations mycelia looping; Thecaspore has two tangible coronal processes, is biconvex mirror shape, and the sporophyte convex surface is obviously coarse, the little wart of tool.
Strain X U08 of the present invention belongs to aerobe, and anti-dry osmophilic strain, optimal pH are 5.2, can grow in the sour environment of pH3-pH6, and optimum growth temperature is 24～30 ℃, and maximum growth temperature is 38 ℃.Can utilize multiple nitrogenous source, carbon source, utilize disaccharide or polysaccharide ability not as utilizing the monose ability strong, in disaccharide and polysaccharide, can only utilize maltose and sucrose.
Bacterial strain of the present invention is in morphologic feature, consistent with the description feature of the diffusing capsule bacterium (Eurotium cristatum) of coronoid process in the fungus identification handbook, and by to can determining behind its dna sequencing that bacterial strain of the present invention bacterial strain of the present invention is the loose novel species of capsule bacterium (Eurotium cristatum) of coronoid process, called after coronoid process capsule bacterium (Eurotium cristatum) XU08 that looses.
After the thalline of the strain X U08 of the present invention that activates on the M40Y slant medium and steamed stuffed bun collected, press the inoculum size that the 100mL substratum adds 0.2-3g thalline and steamed stuffed bun, thalline and the steamed stuffed bun of strain X U08 of the present invention are seeded in M40Y liquid nutrient medium (M40Y liquid nutrient medium: sucrose 400g, malt extract 20g, agar 20g, yeast extract paste 5.0g, water 1000mL, the pH nature, 121 ℃ of sterilization 20min) in, at 24-30 ℃, stirring velocity is that 50-200r/min condition bottom fermentation was cultivated 5-14 days, the fermented liquid that makes can be used in the fermentation manufacturing technique of Fu brick tea, be specially: after Fu brick tea raw material process decatize, before pressing the brick moulding, add the ratio of 5-50mL fermented liquid in the Fu brick tea raw material after the 1kg decatize, add this fermented liquid in the Fu brick tea raw material after decatize, press the brick moulding afterwards again, carry out subsequent production by general Fu brick tea production process after the moulding.
After the thalline of the strain X U08 of the present invention that activates on the M40Y slant medium and steamed stuffed bun collected, press the inoculum size that the 100g substratum adds 0.2-1g thalline and steamed stuffed bun, thalline and the steamed stuffed bun of strain X U08 of the present invention are seeded in the solid fermentation substratum, cultivated 3-7 days at 24-30 ℃, the starter that makes can be used in the fermentation manufacturing technique of Fu brick tea, be specially: after Fu brick tea raw material process decatize, before pressing the brick moulding, add the ratio of 2-40g solid fermentation agent in the Fu brick tea raw material after the 1kg decatize, add this solid fermentation agent in the Fu brick tea raw material after decatize, press the brick moulding again after mixing thoroughly, carry out subsequent production by general Fu brick tea production process after the moulding.
As it is big to produce the solid fermentation dosage that needs, also can utilize the solid fermentation agent of the 1st preparation to carry out 1-2 time enlarged culturing again, addition is the solid fermentation agent that 100g solid fermentation substratum adds the 1st preparation of 2-5g, after mixing thoroughly, be 24-30 ℃ in temperature, cultivated 3-7 days.
The above-mentioned solid fermentation substratum of mentioning contains 20% tea dust, 20% wheat bran, 0.5% malt extract, 0.5% yeast extract paste, 9% sucrose and 50% water by mass ratio, be after tea dust, wheat bran, malt extract, yeast extract paste and sucrose are mixed by mass ratio 40:40:1:1:18, the water of quality such as adding (total mass of tea dust, wheat bran, malt extract, yeast extract paste and sucrose) is mixed thoroughly, 121 ℃, the 20min sterilization makes.
Owing to most of microbe in the Fu brick tea raw material after the decatize is killed or is suppressed, add fermented liquid or the solid fermentation agent of strain X U08 preparation this moment, being beneficial to this bacterium grows in repressed brick tea rapidly and grows dim, pollution and the growth of assorted bacterium in the follow-up fermenting process have been avoided as much as possible, simultaneously, strain X U08 of the present invention grows at the Fu brick tea, but the extracellular enzyme that secreting outside is a large amount of, as polyphenoloxidase, polygalacturonase, cellulase, proteolytic enzyme etc., mass degradation in the Fu brick tea is become the less compound of molecular weight, the oxidation that in this process, takes place, polymerization, degraded, effects such as conversion can improve the processing quality of Fu brick tea, be beneficial to and form the strong distinctive local flavor of Fu brick tea, product stability improves.
Embodiment
The preparation of embodiment 1 bacterial strain of the present invention
Gather the ripe young sprout of a bud four or five leaves of tea tree from the yueyang, hunan area, in making Fu brick tea process, press and grow dim naturally after the brick moulding 3 days the time, take by weighing 25g, place and fill the sterilize triangular flask of 0.1% peptone water of 225 mL, fully vibration 5min behind 10 times of serial dilutions, gets three each 0.1mL of dilution bacteria suspension and coats the M40Y culture medium flat plate, cultivate 7d for 25 ℃, observe colonial morphology, get single bacterium colony line separation and purification, get strain X U08 of the present invention.
Embodiment 2 strain X U08 preparation of fermentation liquid of the present invention
Be inoculated in the M40Y liquid nutrient medium after the thalline of the strain X U08 of the present invention that activates on the M40Y slant medium and steamed stuffed bun collected and cultivate, inoculum size is that every 100mL M40Y liquid nutrient medium adds 1g thalline and steamed stuffed bun, culture temperature is 26 ℃, and stirring velocity is 80r/min, incubation time 10 days.
The preparation of embodiment 3 strain X U08 solid fermentation of the present invention agent
After the thalline of the strain X U08 of the present invention that activates on the M40Y slant medium and steamed stuffed bun collected, be seeded in the solid fermentation substratum, inoculum size is that every 100g substratum adds 0.5 gram thalline and steamed stuffed bun, after mixing thoroughly, cultivates 5 days in 27 ℃.
The preparation of embodiment 4 strain X U08 solid fermentation of the present invention agent
When need producing relatively large solid fermentation agent, after example 3 preparation solid fermentation agent, utilize this solid fermentation agent of preparation to carry out enlarged culturing again 1 time, addition is this solid fermentation agent that every 100g solid fermentation substratum adds the 3g preparation, after mixing thoroughly, cultivated 5 days at 27 ℃.
The application of embodiment 5 fermented liquids of the present invention in Fu brick tea production fermenting process
Get the fermented liquid that makes among the embodiment 2, Fu brick tea raw material through after the decatize, press the brick moulding before, add the amount of 20mL fermented liquid by the Fu brick tea raw material after the 1kg decatize and add this fermented liquid, press the brick moulding afterwards again, carry out subsequent production by general Fu brick tea production process after the moulding.
The application of embodiment 6 solid fermentation agent of the present invention in Fu brick tea production fermenting process
Get the solid fermentation agent that makes among the embodiment 3, Fu brick tea raw material through after the decatize, press the brick moulding before, add the amount of 25g solid fermentation agent by the Fu brick tea raw material after the 1kg decatize and add this solid fermentation agent, press the brick moulding afterwards again, carry out subsequent production by general Fu brick tea production process after the moulding.
Claims (7)
1. the strain tea source coronoid process capsule bacteria strain that looses, it is characterized in that, this bacterial strain called after coronoid process capsule bacterium (Eurotium cristatum) XU08 that looses is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on March 13rd, 2012, and deposit number is: CGMCC No.5891.
2. a fermentation method prepares the method that fermented liquid is used in Fu brick tea production fermentation, it is characterized in that, bacterial strain uses therefor is the diffusing capsule bacterium XU08 of the described coronoid process of claim 1, substratum is the M40Y liquid nutrient medium, fermentation condition: inoculum size is added the diffusing capsule bacterium XU08 of 0.2-3g coronoid process for the 100mLM40Y liquid nutrient medium, incubation time 5-14 days, temperature was 24-30 ℃, and stirring velocity is 50-200r/min.
3. a fermentation method prepares the method that starter is used in Fu brick tea production fermentation, it is characterized in that, bacterial strain uses therefor is the diffusing capsule bacterium XU08 of the described coronoid process of claim 1, substratum is the solid fermentation substratum, fermentation condition: inoculum size is that 100g solid fermentation substratum adds the diffusing capsule bacterium XU08 of 0.2-1g coronoid process, temperature is 24-30 ℃, and incubation time is 3-7 days.
4. method as claimed in claim 3, its spy is that described solid fermentation substratum contains 20% tea dust, 20% wheat bran, 0.5% malt extract, 0.5% yeast extract paste, 9% sucrose and 50% water by mass ratio.
5. the application of bacterial strain as claimed in claim 1 in Fu brick tea production fermentation.
6. as the application of fermented liquid in Fu brick tea production fermentation of method preparation as described in the claim 2.
7. as the application of starter in Fu brick tea production fermentation of method preparation as described in claim 3 or 4.
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865810A (en) * | 2014-03-26 | 2014-06-18 | 湖南城市学院 | Method for culturing golden flower fungus powder by utilizing tea leftovers |
| CN103865811A (en) * | 2014-03-26 | 2014-06-18 | 湖南城市学院 | Process for cyclically preparing eurotium cristarum by adding nutrient solution into tea residues |
| CN104222353A (en) * | 2014-09-22 | 2014-12-24 | 陕西科技大学 | Fuzhuan tea fragrance improving technology |
| CN104286238A (en) * | 2014-09-19 | 2015-01-21 | 陕西科技大学 | Technology for carrying out fungus growing on loose tea |
| CN104286247A (en) * | 2014-09-19 | 2015-01-21 | 陕西科技大学 | Preparation method of detoxified gingko tea |
| CN104928184A (en) * | 2014-03-17 | 2015-09-23 | 中国土产畜产进出口总公司 | Tea-source eurotium cristatum strain and application thereof |
| CN105062984A (en) * | 2015-09-16 | 2015-11-18 | 福建师范大学 | Method for preparing laccase by fungus fermentation |
| CN105087511A (en) * | 2015-10-09 | 2015-11-25 | 福建师范大学 | Culture medium and method for preparing polyphenol oxidase through fungus fermentation process |
| CN105132299A (en) * | 2015-09-07 | 2015-12-09 | 唐红军 | Method for producing saccharomycetes by use of eurotium cristatum and application of produced saccharomycetes |
| CN105441486A (en) * | 2015-12-15 | 2016-03-30 | 贵州大学 | Method for producing foot bath powder from waste tea slag through Eurotium amstelodami |
| CN105746756A (en) * | 2016-02-23 | 2016-07-13 | 中国科学院微生物研究所 | Method of producing Fu tea from artificial fermentation tea leaves and bacterial strain |
| CN106676013A (en) * | 2016-11-24 | 2017-05-17 | 广西中烟工业有限责任公司 | Method for improving quality of redrying tobacco leaves by utilizing fermentation of eurotium cristatum tea leaves |
| CN106834131A (en) * | 2016-11-24 | 2017-06-13 | 广西中烟工业有限责任公司 | Fungi and its application of a kind of degrading tobacco stem pectin and xylan |
| WO2018161216A1 (en) * | 2017-03-06 | 2018-09-13 | 中国茶叶有限公司 | Eurotium cristatum and use thereof |
| CN108719518A (en) * | 2018-06-01 | 2018-11-02 | 益阳茶厂有限公司 | The preparation method and fritter Fu-brick tea of fritter Fu-brick tea |
| CN108740131A (en) * | 2018-08-02 | 2018-11-06 | 湖南农业大学 | A kind of production method of betel nut Fu-brick tea |
| CN109749942A (en) * | 2018-08-02 | 2019-05-14 | 湖南农业大学 | A kind of preparation method of betel nut Fu-brick tea production leavening |
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| CN114574368A (en) * | 2021-12-06 | 2022-06-03 | 湖南农业大学 | Golden flower fungus and application thereof in processing of Fu tea |
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Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104928184B (en) * | 2014-03-17 | 2018-06-01 | 中国土产畜产进出口总公司 | A kind of tea source eurotium cristatum strain and its application |
| CN104928184A (en) * | 2014-03-17 | 2015-09-23 | 中国土产畜产进出口总公司 | Tea-source eurotium cristatum strain and application thereof |
| CN103865811A (en) * | 2014-03-26 | 2014-06-18 | 湖南城市学院 | Process for cyclically preparing eurotium cristarum by adding nutrient solution into tea residues |
| CN103865810A (en) * | 2014-03-26 | 2014-06-18 | 湖南城市学院 | Method for culturing golden flower fungus powder by utilizing tea leftovers |
| CN104286238A (en) * | 2014-09-19 | 2015-01-21 | 陕西科技大学 | Technology for carrying out fungus growing on loose tea |
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| CN104222353A (en) * | 2014-09-22 | 2014-12-24 | 陕西科技大学 | Fuzhuan tea fragrance improving technology |
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| CN110172419A (en) * | 2019-05-13 | 2019-08-27 | 山东省农业科学院农业质量标准与检测技术研究所 | A kind of Extracts from Peanut Hulls, preparation method and application |
| CN114574368A (en) * | 2021-12-06 | 2022-06-03 | 湖南农业大学 | Golden flower fungus and application thereof in processing of Fu tea |
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