CN105746756A - Method of producing Fu tea from artificial fermentation tea leaves and bacterial strain - Google Patents
Method of producing Fu tea from artificial fermentation tea leaves and bacterial strain Download PDFInfo
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- CN105746756A CN105746756A CN201610099134.2A CN201610099134A CN105746756A CN 105746756 A CN105746756 A CN 105746756A CN 201610099134 A CN201610099134 A CN 201610099134A CN 105746756 A CN105746756 A CN 105746756A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/08—Oxidation; Fermentation
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
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- A23F3/06—Treating tea before extraction; Preparations produced thereby
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
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- A23F3/14—Tea preparations, e.g. using additives
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- C12N1/145—Fungal isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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Abstract
The invention discloses a method of producing Fu tea by means of artificial fermentation. Themethod comprises the following steps in sequence: humidifying dry tea leaves to enable water content of the tea leaves to be 30-40%; sterilizing the humidified tea leaves at a temperature of 110-120 DEG C; cooling the sterilized tea leaves to a temperature below 40 DEG C, artificially inoculating activated eurotium cristatum under a sterile condition, and mixing uniformly; fermenting the tea leaves for 20-30 days in a sterile and air-permeable container at a temperature of 20-30 DEG C; hot-pressing and forming the tea leaves; drying the tea leaves naturally. The invention further discloses an eurotium cristatum bacterial strain with the preservation number of CGMCC No.3.15516. According to the invention, the method has the advantages of simplicity in operation, relatively low cost and no limitation from raw materials and geographical conditions. As a sterile culture technology is employed, sundry bacteria contamination is avoided, and high product quality and product safety are realized.
Description
Technical field
The present invention relates to field of microbial fermentation, the method particularly relating to utilize microorganism artificial fermentation Tea Production FUCHA.
Background technology
By Eurotium cristatum (Eurotiumcristatum) (also known as aspergillus cristatus (Aspergilluscristatus), common name FUCHA Eurotium Cristatum) FUCHA of fermenting and producing has adjustment human metabolism, effect of blood fat reducing, blood pressure lowering and adjustment carbohydate metabolism, is the necessary of the daily making milk tea of China's border areas ethnic groups.It specially requires " JINHUA ", and namely the shell of ascus of Eurotium cristatum is generally luxuriant, have delicate fragrance bacterium flower taste, " JINHUA " number be FUCHA quality quality main mark.China's FUCHA makes to produce and adopts natural fermentation, the place of production to be only limitted to Hunan, Hubei, Sichuan, Guangxi and Shaanxi more.
Present stage produce FUCHA to purchase through Folium Camelliae sinensis, screen, wet heap, decatize, suppress, grow dim, 20 multiple working procedures such as drying, process is complicated, relatively costly, and the cycle is longer.Due to natural fermentation, FUCHA produces the restriction being subject to geographical environment, and its raw material adopts local raw dark green tea, and the natural fermentation that raw dark green tea needs at least 1 year could as raw material, and its produce primary limitation in Hunan, Hubei, Sichuan, Guangxi and Shaanxi.And if fermentation condition controls bad to be easy to contaminated mold, such as penicillium and aspergillosis, some mycete can produce harmful mycotoxin.It addition, the FUZHUAN produced can only see JINHUA therein, and JINHUA cannot be seen on surface, affect organoleptic quality.Its production process and quality all can not well control.
Summary of the invention
Present invention mainly solves following technical problem:
1. simplify FUCHA production technology, break industry technology barrier, improve production efficiency, be suitable for promoting.
2. breaking through the restriction of FUCHA raw materials for production and geographical environment, raw materials for production are extensively easy to get, and reduce production cost thus breaking down the barriers between regions and departments and trade monopoly.
3. artificial fermentation replaces natural fermentation can shorten the production cycle, stops living contaminants, makes the production process of FUCHA and quality all controlled.
4. the FUCHA of this ferment making is superior to or equal to like product and national standard (GB/T9833.3 2013) in organoleptic quality and physical and chemical index.
Technical scheme:
The invention provides a kind of method that artificial fermentation produces FUCHA, it in turn includes the following steps:
By dry Folium Camelliae sinensis humidification so that it is water content is 30 40%；
In 110 120 DEG C, by the Folium Camelliae sinensis sterilizing of described humidification；
The Folium Camelliae sinensis of described sterilizing is cooled to 40 DEG C of temperature below, the Eurotium cristatum that aseptically artificial vaccination is activated, and mixes；
In aseptic gas-permeable container, in the temperature of 20 30 DEG C, ferment 20 30 days；
Hot-forming；
Natural drying.
Further, described Folium Camelliae sinensis is the Folium Camelliae sinensis that the leaf of Camellia sinensis (Camelliasinensis) and Pu'er Camellia sinensis (Camelliaassamica) and mutation thereof is made.
Further, described Folium Camelliae sinensis is selected from green tea, raw tea, scented tea, black tea and postfermented tea or its combination.
Further, described Eurotium cristatum is eurotium cristatum strain CGMCCNo.3.15516.
Further, described water content is 35%.
Further, described sterilizing is the steam moist heat sterilization adopting common steamer or high-pressure steam sterilizing pan to carry out.
Further, the temperature of described sterilizing is 110 DEG C, and the time of described sterilizing is about 40 minutes.
Further, the temperature of described fermentation is 25 DEG C.
Further, the time of described fermentation is 25 days.
Further, described hot-forming be that the described Folium Camelliae sinensis fermented is heated approximately to 80 DEG C, then pour into rapidly in mould, be manually pressed into brick tea.
Present invention also offers Eurotium cristatum (Eurotiumcristatum) bacterial strain, it is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCCNo.3.15516.
Describe in detail:
Actication of culture and bacterial strain: strain in-20 DEG C of freezen protective, is transferred rapidly after being taken out by strain and activated in PDA culture dish, it is to avoid inclined-plane melts, and then puts back to and continues Cryopreservation on PDA slant medium.The quantity of activation PDA culture dish is calculated according to inoculum concentration.The culture dish of general 9 millimeters can inoculate 30 conical flasks (triangular flask).Postvaccinal PDA culture dish is cultivated 7 10 days at 25 30 DEG C.
For the Eurotium cristatum of the present invention, also known as aspergillus cristatus, it is isolatable from the old brick tea in the man of peasant household of Huzhu County, Qinghai Province.This bacterial strain is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center (Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3) with proprietary program on 9th in December in 2015, and deposit number is CGMCCNo.3.15516.
Tea raw material gets damp humidification: as long as the leaf of Camellia sinensis and Pu'er Camellia sinensis and mutation thereof after drying all can as raw materials for production.Being placed in 3000 milliliters of conical flasks by 300 grams of dry Folium Camelliae sinensis, Folium Camelliae sinensis carries out spraying humidification, spraying limit, limit is stirred so that it is uniformly, water content is 30 40% to humidification, builds sealed membrane.
Folium Camelliae sinensis sterilizing: can adopt steam moist heat sterilization, the Folium Camelliae sinensis after humidification is placed in common steamer or high-pressure steam sterilizing pan together with conical flask, reaches to carry out after 110 120 DEG C the steam moist heat sterilization of 30-60 minute.
Cooling inoculation: the Folium Camelliae sinensis after sterilizing is cooled to less than 40 DEG C, the Eurotium cristatum that will activate on PDA plate in an aseptic environment, cuts the strain block of about 0.25 square centimeter, is immediately placed in the tea bottle after sterilizing with aseptic inoculation shovel, fasten sealed membrane, firmly concussion and Folium Camelliae sinensis and strain mixing.This process aseptically to carry out, and stops to pollute.
Cultivation and fermentation: ferment 20 30 days in 20 30 DEG C of quiescent culture after inoculation.Now visible a large amount of Eurotium Cristatum covers with Folium Camelliae sinensis, if seeing dark green or pitchy bacterium colony, is penicillium sp or aspergillosis pollution.The Folium Camelliae sinensis polluted just can abandon after must carrying out the steam moist heat sterilization of 40 minutes after 110 120 DEG C, otherwise can cause Factory Building internal contamination.
Hot-forming: the Folium Camelliae sinensis fermented to be heated in common steamer about 80 DEG C, then pours into rapidly in mould, manually to suppress with jack or special pressure tea machine is pressed into brick tea, pressure is 4 6MPa, pressurize 10 minutes.
Natural drying: the brick tea just suppressed keeps flat after sloughing mould, will cover weight thereon and prevent it from expanding, at normal temperature drying, it is not necessary to drying room.Can placing for a long time, the brick tea suppressed still can continue fermentation, and this process is called for after-ripening, at least 1 year.A large amount of JINHUA is had all as seen on brick tea surface and inside after being typically in placement one month.
The main beneficial effect of the present invention:
1. the technology of the present invention is simple to operate, less costly, only through raw material humidification, sterilizing, cooling inoculation, cultivation and fermentation, hot-forming and these steps of natural drying.
2. the present invention is not limited by raw material and geographical conditions, and in office where district all can produce.
3. the present invention adopts Sterile Culture Methods Used, thus having stopped living contaminants, product quality and safety are all very high.
4. the FUCHA that the present invention produces is superior to or equal to the main common FUCHA in market in organoleptic quality and physical and chemical index, and is better than national standard (according to GB/T9833.3 2013), in Table 12.
According to below in conjunction with the accompanying drawing detailed description to the specific embodiment of the invention, those skilled in the art will understand the above-mentioned of the present invention and other purposes, advantage and feature more.
Accompanying drawing explanation
Some specific embodiments of the present invention are hereinafter described in detail with reference to the accompanying drawings by way of example, and not by way of limitation.In accompanying drawing:
Fig. 1: the process chart of the artificial fermenting and producing FUCHA of the present invention.
Detailed description of the invention
In embodiment, raw materials used, reagent, instrument and equipment are those skilled in the art and know, and are market and can buy.
Embodiment 1
1). actication of culture: freezen protective Eurotium cristatum CGMCCNo.3.15516 on PDA (potato dextrose agar) slant medium is transferred in PDA plate, cultivate 7 days at 25 DEG C.
2). choose tea raw material: green tea in bulk, purchased from Ningde City Yan Ting tea processing factory of Fujian Province (other Folium Camelliae sinensis are equally applicable).
3). Folium Camelliae sinensis humidifies: 300 grams of dry Folium Camelliae sinensis are placed in 3000 milliliters of conical flasks, after Folium Camelliae sinensis is carried out spraying humidification so that it is water content is 30%, builds sealed membrane.
4). steam sterilization: the Folium Camelliae sinensis after humidification is placed in common steamer together with conical flask, reach to carry out after 110 120 DEG C the steam moist heat sterilization of 40 minutes.
5). cooling inoculation: the Folium Camelliae sinensis after sterilizing is cooled to less than 40 DEG C, the Eurotium cristatum that will activate on PDA plate in an aseptic environment, cut the strain block of about 0.25 square centimeter with aseptic inoculation shovel, be immediately placed in conical flask, build sealed membrane concussion and Folium Camelliae sinensis mixing.
6). cultivation and fermentation: stand 30 days in 20 DEG C after inoculation.
7). hot-forming: the Folium Camelliae sinensis fermented to be heated in common steamer about to 80 DEG C, pour into rapidly in mould, be manually pressed into brick tea.
8). cover weight room temperature natural drying thereon, can preserve for a long time.The brick tea suppressed still can continue fermentation, and this process is called for after-ripening, at least 1 year, all visible JINHUA growth on brick tea surface and inside.
Embodiment 2
1). actication of culture: freezen protective Eurotium cristatum CGMCCNo.3.15516 on PDA slant medium is transferred in PDA plate, cultivate 7 days at 25 DEG C.
2). choose tea raw material: green tea, purchased from Ningde City Yan Ting tea processing factory of Fujian Province (other Folium Camelliae sinensis are equally applicable).
3). Folium Camelliae sinensis humidifies: 300 grams of dry Folium Camelliae sinensis are placed in 3000 milliliters of conical flasks, after Folium Camelliae sinensis is carried out spraying humidification so that it is water content is 35%, builds sealed membrane.
4). steam sterilization: the Folium Camelliae sinensis after humidification is placed in common steamer together with conical flask, reach to carry out after 110 120 DEG C the steam moist heat sterilization of 40 minutes.
5). cooling inoculation: the Folium Camelliae sinensis after sterilizing is cooled to less than 40 DEG C, the Eurotium cristatum that will activate on PDA plate in an aseptic environment, cut the strain block of about 0.25 square centimeter with aseptic inoculation shovel, be immediately placed in conical flask, build sealed membrane concussion and Folium Camelliae sinensis mixing.
6). cultivation and fermentation: stand 30 days in 20 DEG C after inoculation.
7). hot-forming: the Folium Camelliae sinensis fermented to be heated in common steamer about to 80 DEG C, pour into rapidly in mould, be manually pressed into brick tea.
8). cover weight room temperature natural drying thereon, can preserve for a long time.The brick tea suppressed still can continue fermentation, and this process is called for after-ripening, at least 1 year, all visible JINHUA growth on brick tea surface and inside.
Embodiment 3
1). actication of culture: freezen protective Eurotium cristatum CGMCCNo.3.15516 on PDA slant medium is transferred in PDA plate, cultivate 7 days at 25 DEG C.
2). choose tea raw material: green tea, purchased from Ningde City Yan Ting tea processing factory of Fujian Province (other Folium Camelliae sinensis are equally applicable).
3). Folium Camelliae sinensis humidifies: 300 grams of dry Folium Camelliae sinensis are placed in 3000 milliliters of conical flasks, after Folium Camelliae sinensis is carried out spraying humidification so that it is water content is 40%, builds sealed membrane.
4). steam sterilization: the Folium Camelliae sinensis after humidification is placed in common steamer together with conical flask, reach to carry out after 110 120 DEG C the steam moist heat sterilization of 40 minutes.
5). cooling inoculation: the Folium Camelliae sinensis after sterilizing is cooled to less than 40 DEG C, the Eurotium cristatum that will activate on PDA plate in an aseptic environment, cut the strain block of about 0.25 square centimeter with aseptic inoculation shovel, be immediately placed in conical flask, build sealed membrane concussion and Folium Camelliae sinensis mixing.
6). cultivation and fermentation: stand 30 days in 20 DEG C after inoculation.
7). hot-forming: the Folium Camelliae sinensis fermented to be heated in common steamer about to 80 DEG C, pour into rapidly in mould, be manually pressed into brick tea.
8). cover weight room temperature natural drying thereon, can preserve for a long time.The brick tea suppressed still can continue fermentation, and this process is called for after-ripening, at least 1 year, all visible JINHUA growth on brick tea surface and inside.
Embodiment 4
1). actication of culture: freezen protective Eurotium cristatum CGMCCNo.3.15516 on PDA slant medium is transferred in PDA plate, cultivate 7 days at 25 DEG C.
2). choose tea raw material: green tea, purchased from Ningde City Yan Ting tea processing factory of Fujian Province (other Folium Camelliae sinensis are equally applicable).
3). Folium Camelliae sinensis humidifies: 300 grams of dry Folium Camelliae sinensis are placed in 3000 milliliters of conical flasks, after Folium Camelliae sinensis is carried out spraying humidification so that it is water content is 30%, builds sealed membrane.
4). steam sterilization: the Folium Camelliae sinensis after humidification is placed in common steamer together with conical flask, reach to carry out after 110 120 DEG C the steam moist heat sterilization of 40 minutes.
5). cooling inoculation: the Folium Camelliae sinensis after sterilizing is cooled to less than 40 DEG C, the Eurotium cristatum that will activate on PDA plate in an aseptic environment, cut the strain block of about 0.25 square centimeter with aseptic inoculation shovel, be immediately placed in conical flask, build sealed membrane concussion and Folium Camelliae sinensis mixing.
6). cultivation and fermentation: stand 25 days in 25 DEG C after inoculation.
7). hot-forming: the Folium Camelliae sinensis fermented to be heated in common steamer about to 80 DEG C, pour into rapidly in mould, be manually pressed into brick tea.
8). cover weight room temperature natural drying thereon, can preserve for a long time.The brick tea suppressed still can continue fermentation, and this process is called for after-ripening, at least 1 year, all visible JINHUA growth on brick tea surface and inside.
Embodiment 5
1). actication of culture: freezen protective Eurotium cristatum CGMCCNo.3.15516 on PDA slant medium is transferred in PDA plate, cultivate 7 days at 25 DEG C.
2). choose tea raw material: green tea, purchased from Ningde City Yan Ting tea processing factory of Fujian Province (other Folium Camelliae sinensis are equally applicable).
3). Folium Camelliae sinensis humidifies: 300 grams of dry Folium Camelliae sinensis are placed in 3000 milliliters of conical flasks, after Folium Camelliae sinensis is carried out spraying humidification so that it is water content is 35%, builds sealed membrane.
4). steam sterilization: the Folium Camelliae sinensis after humidification is placed in common steamer together with conical flask, reach to carry out after 110 120 DEG C the steam moist heat sterilization of 40 minutes.
5). cooling inoculation: the Folium Camelliae sinensis after sterilizing is cooled to less than 40 DEG C, the Eurotium cristatum that will activate on PDA plate in an aseptic environment, cut the strain block of about 0.25 square centimeter with aseptic inoculation shovel, be immediately placed in conical flask, build sealed membrane concussion and Folium Camelliae sinensis mixing.
6). cultivation and fermentation: stand 25 days in 25 DEG C after inoculation.
7). hot-forming: the Folium Camelliae sinensis fermented to be heated in common steamer about to 80 DEG C, pour into rapidly in mould, be manually pressed into brick tea.
8). cover weight room temperature natural drying thereon, can preserve for a long time.The brick tea suppressed still can continue fermentation, and this process is called for after-ripening, at least 1 year, all visible JINHUA growth on brick tea surface and inside.
Embodiment 6
1). actication of culture: freezen protective Eurotium cristatum CGMCCNo.3.15516 on PDA slant medium is transferred in PDA plate, cultivate 7 days at 25 DEG C.
2). choose tea raw material: green tea, purchased from Ningde City Yan Ting tea processing factory of Fujian Province (other Folium Camelliae sinensis are equally applicable).
3). Folium Camelliae sinensis humidifies: 300 grams of dry Folium Camelliae sinensis are placed in 3000 milliliters of conical flasks, after Folium Camelliae sinensis is carried out spraying humidification so that it is water content is 40%, builds sealed membrane.
4). steam sterilization: the Folium Camelliae sinensis after humidification is placed in common steamer together with conical flask, reach to carry out after 110 120 DEG C the steam moist heat sterilization of 40 minutes.
5). cooling inoculation: the Folium Camelliae sinensis after sterilizing is cooled to less than 40 DEG C, the Eurotium cristatum that will activate on PDA plate in an aseptic environment, cut the strain block of about 0.25 square centimeter with aseptic inoculation shovel, be immediately placed in conical flask, build sealed membrane concussion and Folium Camelliae sinensis mixing.
6). cultivation and fermentation: stand 25 days in 25 DEG C after inoculation.
7). hot-forming: the Folium Camelliae sinensis fermented to be heated in common steamer about to 80 DEG C, pour into rapidly in mould, be manually pressed into brick tea.
8). cover weight room temperature natural drying thereon, can preserve for a long time.The brick tea suppressed still can continue fermentation, and this process is called for after-ripening, at least 1 year, all visible JINHUA growth on brick tea surface and inside.
Embodiment 7
1). actication of culture: freezen protective Eurotium cristatum CGMCCNo.3.15516 on PDA slant medium is transferred in PDA plate, cultivate 7 days at 25 DEG C.
2). choose tea raw material: green tea, purchased from Ningde City Yan Ting tea processing factory of Fujian Province (other Folium Camelliae sinensis are equally applicable).
3). Folium Camelliae sinensis humidifies: 300 grams of dry Folium Camelliae sinensis are placed in 3000 milliliters of conical flasks, after Folium Camelliae sinensis is carried out spraying humidification so that it is water content is 30%, builds sealed membrane.
4). steam sterilization: the Folium Camelliae sinensis after humidification is placed in common steamer together with conical flask, reach to carry out after 110 120 DEG C the steam moist heat sterilization of 40 minutes.
5). cooling inoculation: the Folium Camelliae sinensis after sterilizing is cooled to less than 40 DEG C, the Eurotium cristatum that will activate on PDA plate in an aseptic environment, cut the strain block of about 0.25 square centimeter with aseptic inoculation shovel, be immediately placed in conical flask, build sealed membrane concussion and Folium Camelliae sinensis mixing.
6). cultivation and fermentation: stand 20 days in 30 DEG C after inoculation.
7). hot-forming: the Folium Camelliae sinensis fermented to be heated in common steamer about to 80 DEG C, pour into rapidly in mould, be manually pressed into brick tea.
8). cover weight room temperature natural drying thereon, can preserve for a long time.The brick tea suppressed still can continue fermentation, and this process is called for after-ripening, at least 1 year, all visible JINHUA growth on brick tea surface and inside.
Embodiment 8
1). actication of culture: freezen protective Eurotium cristatum CGMCCNo.3.15516 on PDA slant medium is transferred in PDA plate, cultivate 7 days at 25 DEG C.
2). choose tea raw material: green tea, purchased from Ningde City Yan Ting tea processing factory of Fujian Province (other Folium Camelliae sinensis are equally applicable).
3). Folium Camelliae sinensis humidifies: 300 grams of dry Folium Camelliae sinensis are placed in 3000 milliliters of conical flasks, after Folium Camelliae sinensis is carried out spraying humidification so that it is water content is 35%, builds sealed membrane.
4). steam sterilization: the Folium Camelliae sinensis after humidification is placed in common steamer together with conical flask, reach to carry out after 110 120 DEG C the steam moist heat sterilization of 40 minutes.
5). cooling inoculation: the Folium Camelliae sinensis after sterilizing is cooled to less than 40 DEG C, the Eurotium cristatum that will activate on PDA plate in an aseptic environment, cut the strain block of about 0.25 square centimeter with aseptic inoculation shovel, be immediately placed in conical flask, build sealed membrane concussion and Folium Camelliae sinensis mixing.
6). cultivation and fermentation: stand 20 days in 30 DEG C after inoculation.
7). hot-forming: the Folium Camelliae sinensis fermented to be heated in common steamer about to 80 DEG C, pour into rapidly in mould, be manually pressed into brick tea.
8). cover weight room temperature natural drying thereon, can preserve for a long time.The brick tea suppressed still can continue fermentation, and this process is called for after-ripening, at least 1 year, all visible JINHUA growth on brick tea surface and inside.
Embodiment 9
1). actication of culture: freezen protective Eurotium cristatum CGMCCNo.3.15516 on PDA slant medium is transferred in PDA plate, cultivate 7 days at 25 DEG C.
2). choose tea raw material: green tea, purchased from Ningde City Yan Ting tea processing factory of Fujian Province (other Folium Camelliae sinensis are equally applicable).
3). Folium Camelliae sinensis humidifies: 300 grams of dry Folium Camelliae sinensis are placed in 3000 milliliters of conical flasks, after Folium Camelliae sinensis is carried out spraying humidification so that it is water content is 40%, builds sealed membrane.
4). steam sterilization: the Folium Camelliae sinensis after humidification is placed in common steamer together with conical flask, reach to carry out after 110 120 DEG C the steam moist heat sterilization of 40 minutes.
5). cooling inoculation: the Folium Camelliae sinensis after sterilizing is cooled to less than 40 DEG C, the Eurotium cristatum that will activate on PDA plate in an aseptic environment, cut the strain block of about 0.25 square centimeter with aseptic inoculation shovel, be immediately placed in conical flask, build sealed membrane concussion and Folium Camelliae sinensis mixing.
6). cultivation and fermentation: stand 20 days in 30 DEG C after inoculation.
7). hot-forming: the Folium Camelliae sinensis fermented to be heated in common steamer about to 80 DEG C, pour into rapidly in mould, be manually pressed into brick tea.
8). cover weight room temperature natural drying thereon, can preserve for a long time.The brick tea suppressed still can continue fermentation, and this process is called for after-ripening, at least 1 year, all visible JINHUA growth on brick tea surface and inside.
The FUCHA produced in above-described embodiment is superior to or equal to the main common Fu-brick tea in market in organoleptic quality and physical and chemical index, and is better than national standard (according to GB/T9833.3 2013), referring specifically to table 12.
The FUCHA that table 1. present invention produces compares (being for 1 term) with the organoleptic quality of presently commercially available Hunan FUCHA and Shaanxi FUCHA.
The FUCHA that table 2 present invention produces compares (being for 1 term) with the physical and chemical index of presently commercially available Hunan FUCHA and Shaanxi FUCHA.
So far, those skilled in the art will recognize that, although the detailed multiple exemplary embodiments illustrate and describing the present invention herein, but, without departing from the spirit and scope of the present invention, still can directly determine according to present disclosure or derive other variations or modifications many meeting the principle of the invention.Therefore, the scope of the present invention is it is understood that cover all these other variations or modifications with regarding as.
Claims (10)
1. the method that artificial fermentation produces FUCHA, it in turn includes the following steps:
By dry Folium Camelliae sinensis humidification so that it is water content is 30 40%；
In 110 120 DEG C, by the Folium Camelliae sinensis sterilizing of described humidification；
The Folium Camelliae sinensis of described sterilizing is cooled to 40 DEG C of temperature below, the Eurotium cristatum (Eurotiumcristatum) that aseptically artificial vaccination is activated, and mix；
In aseptic gas-permeable container, in the temperature of 20 30 DEG C, ferment 20 30 days；
Hot-forming；
Natural drying.
2. method according to claim 1, wherein, described Folium Camelliae sinensis is the Folium Camelliae sinensis that the leaf of Camellia sinensis (Camelliasinensis) and Pu'er Camellia sinensis (Camelliaassamica) and mutation thereof is made.
3. method according to claim 1, wherein, described Folium Camelliae sinensis is selected from green tea, raw tea, scented tea, black tea and postfermented tea or its combination.
4. method according to claim 1, wherein, described Eurotium cristatum is eurotium cristatum strain CGMCCNo.3.15516.
5. the method according to any one of claim 1-4, wherein, described water content is 35%.
6. the method according to any one of claim 1-4, wherein, described sterilizing is the steam moist heat sterilization adopting common steamer or high-pressure steam sterilizing pan to carry out.
7. the method according to any one of claim 1-4, wherein, the temperature of described sterilizing is 110 DEG C, and the time of sterilizing is about 40 minutes.
8. the method according to any one of claim 1-4, wherein, the temperature of described fermentation is 25 DEG C, and the time of described fermentation is 25 days.
9. the method according to any one of claim 1-4, wherein, described hot-forming be that the described Folium Camelliae sinensis fermented is heated approximately to 80 DEG C, then pour into rapidly in mould, be manually pressed into brick tea.
10. eurotium cristatum strain, is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCCNo.3.15516.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106260140A (en) * | 2016-08-24 | 2017-01-04 | 咸阳泾渭茯茶有限公司 | A kind of processing method of FUCHA |
| CN107647011A (en) * | 2017-11-08 | 2018-02-02 | 西北农林科技大学 | A kind of preparation method of no living contaminants Fu tea |
| CN108029829A (en) * | 2017-12-29 | 2018-05-15 | 中南林业科技大学 | A kind of paper mulberry golden flower tea preparation methods |
| CN108967584A (en) * | 2017-06-01 | 2018-12-11 | 勐海茶业有限责任公司 | The method for preparing fermented tea, the fermented tea prepared with this method and its application |
| CN109984227A (en) * | 2019-05-10 | 2019-07-09 | 湖南农业大学 | A kind of Fu tea fermentation process |
| CN110100916A (en) * | 2019-05-08 | 2019-08-09 | 西咸新区茯茶镇茶业有限公司 | A kind of rain and dew tea Fu tea fungus growing process |
| CN113951343A (en) * | 2021-10-15 | 2022-01-21 | 广州市东鹏食品饮料有限公司 | Dried orange peel Pu' er tea and preparation and brewing method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217688A (en) * | 2011-06-23 | 2011-10-19 | 牛乃秀 | Making method of golden flower puer tea |
| CN103275876A (en) * | 2013-05-23 | 2013-09-04 | 湖南农业大学 | Tea source eurotium cristatum strain and application thereof |
| CN103859082A (en) * | 2014-02-26 | 2014-06-18 | 湖南农业大学 | Gold flower big red robe tea and processing technology thereof |
| CN104738256A (en) * | 2013-12-31 | 2015-07-01 | 深圳华大基因科技有限公司 | Fu tea and preparation method and use thereof |
| CN104928184A (en) * | 2014-03-17 | 2015-09-23 | 中国土产畜产进出口总公司 | Tea-source eurotium cristatum strain and application thereof |
| CN105368716A (en) * | 2015-09-18 | 2016-03-02 | 中国土产畜产进出口总公司 | Eurotium cristatum strain and breeding method and application thereof |
-
2016
- 2016-02-23 CN CN201610099134.2A patent/CN105746756A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217688A (en) * | 2011-06-23 | 2011-10-19 | 牛乃秀 | Making method of golden flower puer tea |
| CN103275876A (en) * | 2013-05-23 | 2013-09-04 | 湖南农业大学 | Tea source eurotium cristatum strain and application thereof |
| CN104738256A (en) * | 2013-12-31 | 2015-07-01 | 深圳华大基因科技有限公司 | Fu tea and preparation method and use thereof |
| CN103859082A (en) * | 2014-02-26 | 2014-06-18 | 湖南农业大学 | Gold flower big red robe tea and processing technology thereof |
| CN104928184A (en) * | 2014-03-17 | 2015-09-23 | 中国土产畜产进出口总公司 | Tea-source eurotium cristatum strain and application thereof |
| CN105368716A (en) * | 2015-09-18 | 2016-03-02 | 中国土产畜产进出口总公司 | Eurotium cristatum strain and breeding method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刘武嫦等: "冠突散囊菌对发花黑毛茶品质呈味成分的影响", 《食品安全质量检测学报》 * |
| 李志香: "《食品微生物学及其技能训练》", 31 August 2011 * |
| 李适等: "冠突散囊菌对茶叶品质成分的影响研究 ", 《菌物学报》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106260140A (en) * | 2016-08-24 | 2017-01-04 | 咸阳泾渭茯茶有限公司 | A kind of processing method of FUCHA |
| CN108967584A (en) * | 2017-06-01 | 2018-12-11 | 勐海茶业有限责任公司 | The method for preparing fermented tea, the fermented tea prepared with this method and its application |
| CN107647011A (en) * | 2017-11-08 | 2018-02-02 | 西北农林科技大学 | A kind of preparation method of no living contaminants Fu tea |
| CN108029829A (en) * | 2017-12-29 | 2018-05-15 | 中南林业科技大学 | A kind of paper mulberry golden flower tea preparation methods |
| CN108029829B (en) * | 2017-12-29 | 2021-05-11 | 中南林业科技大学 | Preparation method of paper mulberry golden camellia |
| CN110100916A (en) * | 2019-05-08 | 2019-08-09 | 西咸新区茯茶镇茶业有限公司 | A kind of rain and dew tea Fu tea fungus growing process |
| CN109984227A (en) * | 2019-05-10 | 2019-07-09 | 湖南农业大学 | A kind of Fu tea fermentation process |
| CN113951343A (en) * | 2021-10-15 | 2022-01-21 | 广州市东鹏食品饮料有限公司 | Dried orange peel Pu' er tea and preparation and brewing method thereof |
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