CN107647011A - A kind of preparation method of no living contaminants Fu tea - Google Patents
A kind of preparation method of no living contaminants Fu tea Download PDFInfo
- Publication number
- CN107647011A CN107647011A CN201711088646.XA CN201711088646A CN107647011A CN 107647011 A CN107647011 A CN 107647011A CN 201711088646 A CN201711088646 A CN 201711088646A CN 107647011 A CN107647011 A CN 107647011A
- Authority
- CN
- China
- Prior art keywords
- tea
- capsule bacterium
- tea leaf
- dissipate capsule
- coronoid process
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/08—Oxidation; Fermentation
- A23F3/10—Fermentation with addition of microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/14—Tea preparations, e.g. using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/18—Extraction of water soluble tea constituents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The Fu tea preparation method of no living contaminants disclosed by the invention a kind of is through the following steps that realize:The purebred coronoid process dissipate capsule bacterium of activation culture;Prepare Aqua Folium Camelliae sinensis;Prepare tea leaf powder;Prepare bacteria suspension;Prepare starter tea;It is inoculated with starter tea;It is compressing;Fermented and cultured;Dry ageing.Tealeaves delicate fragrance and special arohid flavour gas can be presented in Fu brick teas.The cycle of existing Fu tea production technologies is foreshortened to 9 10d by the present invention by 30 35d, solves the pollution problem of miscellaneous bacteria in raw material tea leaf, coronoid process dissipate capsule bacterium growth is vigorous in finished product Fu tea, rich in strong tea smell, spore count amount is greatly improved, control Fu tea qualities that can be effective, the stability of Improving The Quality of Products, automatically controlling and standardizing for Fu tea making can be achieved, and be easy to industrialized production.
Description
Technical field
The present invention relates to a kind of preparation method of Fu tea, specifically a kind of preparation method of no living contaminants Fu tea, belong to
Field of food.
Background technology
Fu tea relies primarily on coronoid process dissipate capsule bacterium growth and breeding in tealeaves, will be made after its proper fermentation, drying and ageing.
At present, the production of Fu tea is in the majority with handicraft workshop, though there is scale industrial production, its process conditions still can not effectively prevent raw material
The self-contained living contaminants of tealeaves, causes final product quality unstable.Embody in the following areas:Traditional handicraft is first to original
Expect tealeaves removal of impurities, cake of press, spontaneous fermentation are carried out again after the processing of nest heap;Although adding flash process in the industrialized production of part,
But the processing mode can not effectively remove the miscellaneous bacterias such as aspergillus niger, mould and the spore bacteria in raw material tea leaf;Traditional Fu tea life
Production relies primarily on the coronoid process dissipate capsule bacterium spontaneous fermentation in environment, if the miscellaneous bacteria in raw material tea leaf is not killed, in fermentation process
Miscellaneous bacteria will grow with coronoid process dissipate capsule bacterium symbiosis, a large amount of miscellaneous bacterias be present in finished product Fu tea, cause poor product quality or fermentation failure;From
So fermentation is had a great influence by production environment and the condition such as the spore concentration of coronoid process dissipate capsule bacterium, temperature and humidity in room of growing dim, and is fermented
Condition can not be controlled effectively, cause coronoid process dissipate capsule bacterium skewness in Fu tea, recall rate is low or fermentation failure, living contaminants compare
Seriously, and it is subject to seasonal restrictions, product quality is extremely unstable;Traditional spontaneous fermentation time generally requires 30-35 days, the production cycle
Long, cost is higher.
The content of the invention
The purpose of the present invention is the defects of overcoming present in current Fu tea preparation method, and discloses a kind of no living contaminants
Fu tea preparation methods.
The present invention is through the following steps that realize:
A. the purebred coronoid process dissipate capsule bacterium of activation culture:By coronoid process dissipate capsule bacterium streak inoculation to PDA agar plate surfaces, in 25-28 DEG C
4-6 d are cultivated, terminates culture after coronoid process dissipate capsule bacterium grows golden yellow spore, purebred coronoid process dissipate capsule bacterium is made;
B. Aqua Folium Camelliae sinensis are prepared:A certain amount of raw material tea leaf is weighed, is added in the drinking water of 25-30 times of its weight and stirs,
10-15min is boiled, filters tealeaves, filtrate puts 121-126 DEG C of sterilizing 10-15min, cool to room temperature under aseptic condition, and tealeaves is made
Water is standby;
C. tea leaf powder is prepared:A certain amount of raw material tea leaf is put into 121-126 DEG C of sterilizing 20-30min, then in 60-80 DEG C of dry 3-
5h, 60-100 mesh sieves are crossed after crushing, tea leaf powder is made;
D. bacteria suspension is prepared:The Aqua Folium Camelliae sinensis that will sterilize in right amount are aseptically added in purebred coronoid process dissipate capsule bacterium, use aseptic cotton carrier
Or oese gently scrapes lawn, mycelia and spore are removed, bacteria suspension is made;
E. starter tea is prepared:Bacteria suspension is added in the sterilizing tea leaf powder of 3-5 times of its weight, stirred, 25-28 DEG C of culture
4-6 d, treat tea leaf powder surface and inside grow be evenly distributed, macroscopic golden yellow spore;
F. it is inoculated with starter tea:Starter tea and raw material tea leaf are pressed 1:8-1:10 ratio mixes, 60-80 DEG C of sterilizing 2-3h;
G. it is compressing:Inoculation starter tea is inserted in the sterile Fu tea bags pack of briquetting grinding tool, is pressed into Fu brick teas, Fu brick teas are big
It is small to be determined by briquetting grinding tool inner chamber size;
H. fermented and cultured:Fu brick teas are placed in 25-28 DEG C, relative humidity 70%-80% culturing room fermented and cultured 5-7d, fermented the phase
Between temperature and humidity keep constant, treat that Fu brick teas surface and inside are covered with visible, the equally distributed golden yellow coronoid process of naked eyes and dissipate capsule
Bacterium spore, which ferments, to be completed;
I. ageing is dried:The Fu brick teas completed that will ferment dry 48-72h under 60-65 DEG C of aseptic condition, are placed in after fully drying
It is aged under room temperature condition, tealeaves delicate fragrance and special arohid flavour gas can be presented in ageing process Fu brick teas.
The cycle of existing Fu tea production technologies is foreshortened to 9-10d by the present invention by 30-35d, it is crucial that solving original
Expect the pollution problem of miscellaneous bacteria in tealeaves, coronoid process dissipate capsule bacterium growth is vigorous in finished product Fu tea, rich in strong tea smell, spore quantity
Be greatly improved, control Fu tea qualities that can be effective, the stability of Improving The Quality of Products, can be achieved that Fu tea makes from
Dynamic control and standardization, and it is easy to industrialized production.
Embodiment
With reference to embodiment, the invention will be further described, and embodiment is served only for explaining the present invention, will not be to this hair
It is bright to form any restriction:
A. the purebred coronoid process dissipate capsule bacterium of activation culture:By coronoid process dissipate capsule bacterium streak inoculation to PDA agar plate surfaces, in 25-28 DEG C
5-6 d are cultivated, terminates culture after coronoid process dissipate capsule bacterium grows golden yellow spore, purebred coronoid process dissipate capsule bacterium is made;
B. Aqua Folium Camelliae sinensis are prepared:A certain amount of raw material tea leaf is weighed, is added in the drinking water of 30 times of its weight and stirs, boil
12min, filters tealeaves, and filtrate puts 121 DEG C of sterilizing 10min, cool to room temperature under aseptic condition, and it is standby that Aqua Folium Camelliae sinensis are made;
C. tea leaf powder is prepared:A certain amount of raw material tea leaf is put into 121 DEG C of sterilizing 20min, then in 80 DEG C of dry 3h, 60 are crossed after crushing
Mesh sieve, tea leaf powder is made;
D. bacteria suspension is prepared:The Aqua Folium Camelliae sinensis that will sterilize in right amount are aseptically added in purebred coronoid process dissipate capsule bacterium, use aseptic cotton carrier
Or oese gently scrapes lawn, mycelia and spore are removed, bacteria suspension is made;
E. starter tea is prepared:Bacteria suspension is added in the sterilizing tea leaf powder of 3 times of its weight, stirred, 28 DEG C of culture 5-6d,
Treat tea leaf powder surface and inside grow be evenly distributed, macroscopic golden yellow spore;
F. it is inoculated with starter tea:Starter tea and raw material tea leaf are pressed 1:10 ratio mixes, 80 DEG C of sterilizing 2h;
G. it is compressing:Inoculation starter tea is inserted in the sterile Fu tea bags pack of briquetting grinding tool, is pressed into Fu brick teas, Fu brick teas are big
It is small to be determined by briquetting grinding tool inner chamber size;
H. fermented and cultured:Fu brick teas are placed in 28 DEG C, the culturing room fermented and cultured 6-7d of relative humidity 80%, temperature during fermentation
Keep constant with humidity, treat that Fu brick teas surface is covered with visible, the equally distributed golden yellow coronoid process dissipate capsule bacterium spore of naked eyes with inside
I.e. fermentation is completed;
I. ageing is dried:The Fu brick teas completed that will ferment dry 72h under 65 DEG C of aseptic conditions, and room temperature bar is placed in after fully drying
It is aged under part, tealeaves delicate fragrance and special arohid flavour gas can be presented in ageing process Fu brick teas.
Claims (2)
1. the Fu tea preparation methods of a kind of no living contaminants, it is characterised in that through the following steps that realizing:
A. the purebred coronoid process dissipate capsule bacterium of activation culture:By coronoid process dissipate capsule bacterium streak inoculation to PDA agar plate surfaces, in 25-28 DEG C
4-6 d are cultivated, terminates culture after coronoid process dissipate capsule bacterium grows golden yellow spore, purebred coronoid process dissipate capsule bacterium is made;
B. Aqua Folium Camelliae sinensis are prepared:A certain amount of raw material tea leaf is weighed, is added in the drinking water of 25-30 times of its weight and stirs,
10-15min is boiled, filters tealeaves, filtrate puts 121-126 DEG C of sterilizing 10-15min, cool to room temperature under aseptic condition, and tealeaves is made
Water is standby;
C. tea leaf powder is prepared:A certain amount of raw material tea leaf is put into 121-126 DEG C of sterilizing 20-30min, then in 60-80 DEG C of dry 3-
5h, 60-100 mesh sieves are crossed after crushing, tea leaf powder is made;
D. bacteria suspension is prepared:The Aqua Folium Camelliae sinensis that will sterilize in right amount are aseptically added in purebred coronoid process dissipate capsule bacterium, use aseptic cotton carrier
Or oese gently scrapes lawn, mycelia and spore are removed, bacteria suspension is made;
E. starter tea is prepared:Bacteria suspension is added in the sterilizing tea leaf powder of 3-5 times of its weight, stirred, 25-28 DEG C of culture
4-6 d, treat tea leaf powder surface and inside grow be evenly distributed, macroscopic golden yellow spore;
F. it is inoculated with starter tea:Starter tea and raw material tea leaf are pressed 1:8-1:10 ratio mixes, 60-80 DEG C of sterilizing 2-3h;
G. it is compressing:Inoculation starter tea is inserted in the sterile Fu tea bags pack of briquetting grinding tool, is pressed into Fu brick teas, Fu brick teas are big
It is small to be determined by briquetting grinding tool inner chamber size;
H. fermented and cultured:Fu brick teas are placed in 25-28 DEG C, relative humidity 70%-80% culturing room fermented and cultured 5-7d, fermented the phase
Between temperature and humidity keep constant, treat that Fu brick teas surface and inside are covered with visible, the equally distributed golden yellow coronoid process of naked eyes and dissipate capsule
Bacterium spore, which ferments, to be completed;
I. ageing is dried:The Fu brick teas completed that will ferment dry 48-72h under 60-65 DEG C of aseptic condition, are placed in after fully drying
It is aged under room temperature condition, tealeaves delicate fragrance and special arohid flavour gas can be presented in ageing process Fu brick teas.
2. the Fu tea preparation methods of a kind of no living contaminants according to claim 1, it is characterised in that be by following step
It is rapid to realize:
A. the purebred coronoid process dissipate capsule bacterium of activation culture:By coronoid process dissipate capsule bacterium streak inoculation to PDA agar plate surfaces, in 25-28 DEG C
5-6 d are cultivated, terminates culture after coronoid process dissipate capsule bacterium grows golden yellow spore, purebred coronoid process dissipate capsule bacterium is made;
B. Aqua Folium Camelliae sinensis are prepared:A certain amount of raw material tea leaf is weighed, is added in the drinking water of 30 times of its weight and stirs, boil
12min, filters tealeaves, and filtrate puts 121 DEG C of sterilizing 10min, cool to room temperature under aseptic condition, and it is standby that Aqua Folium Camelliae sinensis are made;
C. tea leaf powder is prepared:A certain amount of raw material tea leaf is put into 121 DEG C of sterilizing 20min, then in 80 DEG C of dry 3h, 60 are crossed after crushing
Mesh sieve, tea leaf powder is made;
D. bacteria suspension is prepared:The Aqua Folium Camelliae sinensis that will sterilize in right amount are aseptically added in purebred coronoid process dissipate capsule bacterium, use aseptic cotton carrier
Or oese gently scrapes lawn, mycelia and spore are removed, bacteria suspension is made;
E. starter tea is prepared:Bacteria suspension is added in the sterilizing tea leaf powder of 3 times of its weight, stirred, 28 DEG C of culture 5-6d,
Treat tea leaf powder surface and inside grow be evenly distributed, macroscopic golden yellow spore;
F. it is inoculated with starter tea:Starter tea and raw material tea leaf are pressed 1:10 ratio mixes, 80 DEG C of sterilizing 2h;
G. it is compressing:Inoculation starter tea is inserted in the sterile Fu tea bags pack of briquetting grinding tool, is pressed into Fu brick teas, Fu brick teas are big
It is small to be determined by briquetting grinding tool inner chamber size;
H. fermented and cultured:Fu brick teas are placed in 28 DEG C, the culturing room fermented and cultured 6-7d of relative humidity 80%, temperature during fermentation
Keep constant with humidity, treat that Fu brick teas surface is covered with visible, the equally distributed golden yellow coronoid process dissipate capsule bacterium spore of naked eyes with inside
I.e. fermentation is completed;
I. ageing is dried:The Fu brick teas completed that will ferment dry 72h under 65 DEG C of aseptic conditions, and room temperature bar is placed in after fully drying
It is aged under part, tealeaves delicate fragrance and special arohid flavour gas can be presented in ageing process Fu brick teas.
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CN201711088646.XA CN107647011A (en) | 2017-11-08 | 2017-11-08 | A kind of preparation method of no living contaminants Fu tea |
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CN201711088646.XA CN107647011A (en) | 2017-11-08 | 2017-11-08 | A kind of preparation method of no living contaminants Fu tea |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108935791A (en) * | 2018-09-03 | 2018-12-07 | 西安交通大学 | One kind can standardized scattered Fu tea production technology |
CN109984227A (en) * | 2019-05-10 | 2019-07-09 | 湖南农业大学 | A kind of Fu tea fermentation process |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01257430A (en) * | 1988-04-07 | 1989-10-13 | House Food Ind Co Ltd | Aseptic green tea |
CN102084904A (en) * | 2010-12-21 | 2011-06-08 | 湖南城市学院 | Preparation technology for fu brick tea flowering loosing tea |
CN102120963A (en) * | 2010-12-21 | 2011-07-13 | 湖南城市学院 | Rapid separation method of eurotium cristatum |
CN105746756A (en) * | 2016-02-23 | 2016-07-13 | 中国科学院微生物研究所 | Method of producing Fu tea from artificial fermentation tea leaves and bacterial strain |
-
2017
- 2017-11-08 CN CN201711088646.XA patent/CN107647011A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01257430A (en) * | 1988-04-07 | 1989-10-13 | House Food Ind Co Ltd | Aseptic green tea |
CN102084904A (en) * | 2010-12-21 | 2011-06-08 | 湖南城市学院 | Preparation technology for fu brick tea flowering loosing tea |
CN102120963A (en) * | 2010-12-21 | 2011-07-13 | 湖南城市学院 | Rapid separation method of eurotium cristatum |
CN105746756A (en) * | 2016-02-23 | 2016-07-13 | 中国科学院微生物研究所 | Method of producing Fu tea from artificial fermentation tea leaves and bacterial strain |
Non-Patent Citations (1)
Title |
---|
高鸿鹄等著: "《甘蔗糖蜜及其酒精废液资源化处理技术研究》", 31 January 2012, 北京:中国环境科学出版社 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108935791A (en) * | 2018-09-03 | 2018-12-07 | 西安交通大学 | One kind can standardized scattered Fu tea production technology |
CN109984227A (en) * | 2019-05-10 | 2019-07-09 | 湖南农业大学 | A kind of Fu tea fermentation process |
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Application publication date: 20180202 |
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