CN107647011A - A kind of preparation method of no living contaminants Fu tea - Google Patents

A kind of preparation method of no living contaminants Fu tea Download PDF

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Publication number
CN107647011A
CN107647011A CN201711088646.XA CN201711088646A CN107647011A CN 107647011 A CN107647011 A CN 107647011A CN 201711088646 A CN201711088646 A CN 201711088646A CN 107647011 A CN107647011 A CN 107647011A
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CN
China
Prior art keywords
tea
capsule bacterium
tea leaf
dissipate capsule
coronoid process
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201711088646.XA
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Chinese (zh)
Inventor
杨保伟
孟令缘
盛敏
葛武鹏
张强
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Northwest A&F University
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Northwest A&F University
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Filing date
Publication date
Application filed by Northwest A&F University filed Critical Northwest A&F University
Priority to CN201711088646.XA priority Critical patent/CN107647011A/en
Publication of CN107647011A publication Critical patent/CN107647011A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/06Treating tea before extraction; Preparations produced thereby
    • A23F3/08Oxidation; Fermentation
    • A23F3/10Fermentation with addition of microorganisms or enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/06Treating tea before extraction; Preparations produced thereby
    • A23F3/14Tea preparations, e.g. using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/16Tea extraction; Tea extracts; Treating tea extract; Making instant tea
    • A23F3/18Extraction of water soluble tea constituents
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor

Abstract

The Fu tea preparation method of no living contaminants disclosed by the invention a kind of is through the following steps that realize:The purebred coronoid process dissipate capsule bacterium of activation culture;Prepare Aqua Folium Camelliae sinensis;Prepare tea leaf powder;Prepare bacteria suspension;Prepare starter tea;It is inoculated with starter tea;It is compressing;Fermented and cultured;Dry ageing.Tealeaves delicate fragrance and special arohid flavour gas can be presented in Fu brick teas.The cycle of existing Fu tea production technologies is foreshortened to 9 10d by the present invention by 30 35d, solves the pollution problem of miscellaneous bacteria in raw material tea leaf, coronoid process dissipate capsule bacterium growth is vigorous in finished product Fu tea, rich in strong tea smell, spore count amount is greatly improved, control Fu tea qualities that can be effective, the stability of Improving The Quality of Products, automatically controlling and standardizing for Fu tea making can be achieved, and be easy to industrialized production.

Description

A kind of preparation method of no living contaminants Fu tea
Technical field
The present invention relates to a kind of preparation method of Fu tea, specifically a kind of preparation method of no living contaminants Fu tea, belong to Field of food.
Background technology
Fu tea relies primarily on coronoid process dissipate capsule bacterium growth and breeding in tealeaves, will be made after its proper fermentation, drying and ageing. At present, the production of Fu tea is in the majority with handicraft workshop, though there is scale industrial production, its process conditions still can not effectively prevent raw material The self-contained living contaminants of tealeaves, causes final product quality unstable.Embody in the following areas:Traditional handicraft is first to original Expect tealeaves removal of impurities, cake of press, spontaneous fermentation are carried out again after the processing of nest heap;Although adding flash process in the industrialized production of part, But the processing mode can not effectively remove the miscellaneous bacterias such as aspergillus niger, mould and the spore bacteria in raw material tea leaf;Traditional Fu tea life Production relies primarily on the coronoid process dissipate capsule bacterium spontaneous fermentation in environment, if the miscellaneous bacteria in raw material tea leaf is not killed, in fermentation process Miscellaneous bacteria will grow with coronoid process dissipate capsule bacterium symbiosis, a large amount of miscellaneous bacterias be present in finished product Fu tea, cause poor product quality or fermentation failure;From So fermentation is had a great influence by production environment and the condition such as the spore concentration of coronoid process dissipate capsule bacterium, temperature and humidity in room of growing dim, and is fermented Condition can not be controlled effectively, cause coronoid process dissipate capsule bacterium skewness in Fu tea, recall rate is low or fermentation failure, living contaminants compare Seriously, and it is subject to seasonal restrictions, product quality is extremely unstable;Traditional spontaneous fermentation time generally requires 30-35 days, the production cycle Long, cost is higher.
The content of the invention
The purpose of the present invention is the defects of overcoming present in current Fu tea preparation method, and discloses a kind of no living contaminants Fu tea preparation methods.
The present invention is through the following steps that realize:
A. the purebred coronoid process dissipate capsule bacterium of activation culture:By coronoid process dissipate capsule bacterium streak inoculation to PDA agar plate surfaces, in 25-28 DEG C 4-6 d are cultivated, terminates culture after coronoid process dissipate capsule bacterium grows golden yellow spore, purebred coronoid process dissipate capsule bacterium is made;
B. Aqua Folium Camelliae sinensis are prepared:A certain amount of raw material tea leaf is weighed, is added in the drinking water of 25-30 times of its weight and stirs, 10-15min is boiled, filters tealeaves, filtrate puts 121-126 DEG C of sterilizing 10-15min, cool to room temperature under aseptic condition, and tealeaves is made Water is standby;
C. tea leaf powder is prepared:A certain amount of raw material tea leaf is put into 121-126 DEG C of sterilizing 20-30min, then in 60-80 DEG C of dry 3- 5h, 60-100 mesh sieves are crossed after crushing, tea leaf powder is made;
D. bacteria suspension is prepared:The Aqua Folium Camelliae sinensis that will sterilize in right amount are aseptically added in purebred coronoid process dissipate capsule bacterium, use aseptic cotton carrier Or oese gently scrapes lawn, mycelia and spore are removed, bacteria suspension is made;
E. starter tea is prepared:Bacteria suspension is added in the sterilizing tea leaf powder of 3-5 times of its weight, stirred, 25-28 DEG C of culture 4-6 d, treat tea leaf powder surface and inside grow be evenly distributed, macroscopic golden yellow spore;
F. it is inoculated with starter tea:Starter tea and raw material tea leaf are pressed 1:8-1:10 ratio mixes, 60-80 DEG C of sterilizing 2-3h;
G. it is compressing:Inoculation starter tea is inserted in the sterile Fu tea bags pack of briquetting grinding tool, is pressed into Fu brick teas, Fu brick teas are big It is small to be determined by briquetting grinding tool inner chamber size;
H. fermented and cultured:Fu brick teas are placed in 25-28 DEG C, relative humidity 70%-80% culturing room fermented and cultured 5-7d, fermented the phase Between temperature and humidity keep constant, treat that Fu brick teas surface and inside are covered with visible, the equally distributed golden yellow coronoid process of naked eyes and dissipate capsule Bacterium spore, which ferments, to be completed;
I. ageing is dried:The Fu brick teas completed that will ferment dry 48-72h under 60-65 DEG C of aseptic condition, are placed in after fully drying It is aged under room temperature condition, tealeaves delicate fragrance and special arohid flavour gas can be presented in ageing process Fu brick teas.
The cycle of existing Fu tea production technologies is foreshortened to 9-10d by the present invention by 30-35d, it is crucial that solving original Expect the pollution problem of miscellaneous bacteria in tealeaves, coronoid process dissipate capsule bacterium growth is vigorous in finished product Fu tea, rich in strong tea smell, spore quantity Be greatly improved, control Fu tea qualities that can be effective, the stability of Improving The Quality of Products, can be achieved that Fu tea makes from Dynamic control and standardization, and it is easy to industrialized production.
Embodiment
With reference to embodiment, the invention will be further described, and embodiment is served only for explaining the present invention, will not be to this hair It is bright to form any restriction:
A. the purebred coronoid process dissipate capsule bacterium of activation culture:By coronoid process dissipate capsule bacterium streak inoculation to PDA agar plate surfaces, in 25-28 DEG C 5-6 d are cultivated, terminates culture after coronoid process dissipate capsule bacterium grows golden yellow spore, purebred coronoid process dissipate capsule bacterium is made;
B. Aqua Folium Camelliae sinensis are prepared:A certain amount of raw material tea leaf is weighed, is added in the drinking water of 30 times of its weight and stirs, boil 12min, filters tealeaves, and filtrate puts 121 DEG C of sterilizing 10min, cool to room temperature under aseptic condition, and it is standby that Aqua Folium Camelliae sinensis are made;
C. tea leaf powder is prepared:A certain amount of raw material tea leaf is put into 121 DEG C of sterilizing 20min, then in 80 DEG C of dry 3h, 60 are crossed after crushing Mesh sieve, tea leaf powder is made;
D. bacteria suspension is prepared:The Aqua Folium Camelliae sinensis that will sterilize in right amount are aseptically added in purebred coronoid process dissipate capsule bacterium, use aseptic cotton carrier Or oese gently scrapes lawn, mycelia and spore are removed, bacteria suspension is made;
E. starter tea is prepared:Bacteria suspension is added in the sterilizing tea leaf powder of 3 times of its weight, stirred, 28 DEG C of culture 5-6d, Treat tea leaf powder surface and inside grow be evenly distributed, macroscopic golden yellow spore;
F. it is inoculated with starter tea:Starter tea and raw material tea leaf are pressed 1:10 ratio mixes, 80 DEG C of sterilizing 2h;
G. it is compressing:Inoculation starter tea is inserted in the sterile Fu tea bags pack of briquetting grinding tool, is pressed into Fu brick teas, Fu brick teas are big It is small to be determined by briquetting grinding tool inner chamber size;
H. fermented and cultured:Fu brick teas are placed in 28 DEG C, the culturing room fermented and cultured 6-7d of relative humidity 80%, temperature during fermentation Keep constant with humidity, treat that Fu brick teas surface is covered with visible, the equally distributed golden yellow coronoid process dissipate capsule bacterium spore of naked eyes with inside I.e. fermentation is completed;
I. ageing is dried:The Fu brick teas completed that will ferment dry 72h under 65 DEG C of aseptic conditions, and room temperature bar is placed in after fully drying It is aged under part, tealeaves delicate fragrance and special arohid flavour gas can be presented in ageing process Fu brick teas.

Claims (2)

1. the Fu tea preparation methods of a kind of no living contaminants, it is characterised in that through the following steps that realizing:
A. the purebred coronoid process dissipate capsule bacterium of activation culture:By coronoid process dissipate capsule bacterium streak inoculation to PDA agar plate surfaces, in 25-28 DEG C 4-6 d are cultivated, terminates culture after coronoid process dissipate capsule bacterium grows golden yellow spore, purebred coronoid process dissipate capsule bacterium is made;
B. Aqua Folium Camelliae sinensis are prepared:A certain amount of raw material tea leaf is weighed, is added in the drinking water of 25-30 times of its weight and stirs, 10-15min is boiled, filters tealeaves, filtrate puts 121-126 DEG C of sterilizing 10-15min, cool to room temperature under aseptic condition, and tealeaves is made Water is standby;
C. tea leaf powder is prepared:A certain amount of raw material tea leaf is put into 121-126 DEG C of sterilizing 20-30min, then in 60-80 DEG C of dry 3- 5h, 60-100 mesh sieves are crossed after crushing, tea leaf powder is made;
D. bacteria suspension is prepared:The Aqua Folium Camelliae sinensis that will sterilize in right amount are aseptically added in purebred coronoid process dissipate capsule bacterium, use aseptic cotton carrier Or oese gently scrapes lawn, mycelia and spore are removed, bacteria suspension is made;
E. starter tea is prepared:Bacteria suspension is added in the sterilizing tea leaf powder of 3-5 times of its weight, stirred, 25-28 DEG C of culture 4-6 d, treat tea leaf powder surface and inside grow be evenly distributed, macroscopic golden yellow spore;
F. it is inoculated with starter tea:Starter tea and raw material tea leaf are pressed 1:8-1:10 ratio mixes, 60-80 DEG C of sterilizing 2-3h;
G. it is compressing:Inoculation starter tea is inserted in the sterile Fu tea bags pack of briquetting grinding tool, is pressed into Fu brick teas, Fu brick teas are big It is small to be determined by briquetting grinding tool inner chamber size;
H. fermented and cultured:Fu brick teas are placed in 25-28 DEG C, relative humidity 70%-80% culturing room fermented and cultured 5-7d, fermented the phase Between temperature and humidity keep constant, treat that Fu brick teas surface and inside are covered with visible, the equally distributed golden yellow coronoid process of naked eyes and dissipate capsule Bacterium spore, which ferments, to be completed;
I. ageing is dried:The Fu brick teas completed that will ferment dry 48-72h under 60-65 DEG C of aseptic condition, are placed in after fully drying It is aged under room temperature condition, tealeaves delicate fragrance and special arohid flavour gas can be presented in ageing process Fu brick teas.
2. the Fu tea preparation methods of a kind of no living contaminants according to claim 1, it is characterised in that be by following step It is rapid to realize:
A. the purebred coronoid process dissipate capsule bacterium of activation culture:By coronoid process dissipate capsule bacterium streak inoculation to PDA agar plate surfaces, in 25-28 DEG C 5-6 d are cultivated, terminates culture after coronoid process dissipate capsule bacterium grows golden yellow spore, purebred coronoid process dissipate capsule bacterium is made;
B. Aqua Folium Camelliae sinensis are prepared:A certain amount of raw material tea leaf is weighed, is added in the drinking water of 30 times of its weight and stirs, boil 12min, filters tealeaves, and filtrate puts 121 DEG C of sterilizing 10min, cool to room temperature under aseptic condition, and it is standby that Aqua Folium Camelliae sinensis are made;
C. tea leaf powder is prepared:A certain amount of raw material tea leaf is put into 121 DEG C of sterilizing 20min, then in 80 DEG C of dry 3h, 60 are crossed after crushing Mesh sieve, tea leaf powder is made;
D. bacteria suspension is prepared:The Aqua Folium Camelliae sinensis that will sterilize in right amount are aseptically added in purebred coronoid process dissipate capsule bacterium, use aseptic cotton carrier Or oese gently scrapes lawn, mycelia and spore are removed, bacteria suspension is made;
E. starter tea is prepared:Bacteria suspension is added in the sterilizing tea leaf powder of 3 times of its weight, stirred, 28 DEG C of culture 5-6d, Treat tea leaf powder surface and inside grow be evenly distributed, macroscopic golden yellow spore;
F. it is inoculated with starter tea:Starter tea and raw material tea leaf are pressed 1:10 ratio mixes, 80 DEG C of sterilizing 2h;
G. it is compressing:Inoculation starter tea is inserted in the sterile Fu tea bags pack of briquetting grinding tool, is pressed into Fu brick teas, Fu brick teas are big It is small to be determined by briquetting grinding tool inner chamber size;
H. fermented and cultured:Fu brick teas are placed in 28 DEG C, the culturing room fermented and cultured 6-7d of relative humidity 80%, temperature during fermentation Keep constant with humidity, treat that Fu brick teas surface is covered with visible, the equally distributed golden yellow coronoid process dissipate capsule bacterium spore of naked eyes with inside I.e. fermentation is completed;
I. ageing is dried:The Fu brick teas completed that will ferment dry 72h under 65 DEG C of aseptic conditions, and room temperature bar is placed in after fully drying It is aged under part, tealeaves delicate fragrance and special arohid flavour gas can be presented in ageing process Fu brick teas.
CN201711088646.XA 2017-11-08 2017-11-08 A kind of preparation method of no living contaminants Fu tea Pending CN107647011A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108935791A (en) * 2018-09-03 2018-12-07 西安交通大学 One kind can standardized scattered Fu tea production technology
CN109984227A (en) * 2019-05-10 2019-07-09 湖南农业大学 A kind of Fu tea fermentation process

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01257430A (en) * 1988-04-07 1989-10-13 House Food Ind Co Ltd Aseptic green tea
CN102084904A (en) * 2010-12-21 2011-06-08 湖南城市学院 Preparation technology for fu brick tea flowering loosing tea
CN102120963A (en) * 2010-12-21 2011-07-13 湖南城市学院 Rapid separation method of eurotium cristatum
CN105746756A (en) * 2016-02-23 2016-07-13 中国科学院微生物研究所 Method of producing Fu tea from artificial fermentation tea leaves and bacterial strain

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01257430A (en) * 1988-04-07 1989-10-13 House Food Ind Co Ltd Aseptic green tea
CN102084904A (en) * 2010-12-21 2011-06-08 湖南城市学院 Preparation technology for fu brick tea flowering loosing tea
CN102120963A (en) * 2010-12-21 2011-07-13 湖南城市学院 Rapid separation method of eurotium cristatum
CN105746756A (en) * 2016-02-23 2016-07-13 中国科学院微生物研究所 Method of producing Fu tea from artificial fermentation tea leaves and bacterial strain

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
高鸿鹄等著: "《甘蔗糖蜜及其酒精废液资源化处理技术研究》", 31 January 2012, 北京:中国环境科学出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108935791A (en) * 2018-09-03 2018-12-07 西安交通大学 One kind can standardized scattered Fu tea production technology
CN109984227A (en) * 2019-05-10 2019-07-09 湖南农业大学 A kind of Fu tea fermentation process

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