CN104274538B - Traditional Chinese medicine extract for treating gout and preparation method thereof - Google Patents
Traditional Chinese medicine extract for treating gout and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/21—Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/254—Acanthopanax or Eleutherococcus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/66—Papaveraceae (Poppy family), e.g. bloodroot
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention belongs to the field of a traditional Chinese medicine, and discloses a traditional Chinese medicine extract for treating gout and a preparation method thereof. The traditional Chinese medicine extract includes the following extracts in parts by weight: 4-10 parts of an angelica aqueous extract, 7-13 parts of a pseudo-ginseng aqueous extract, 5-10 parts of a rhizoma corydalis ethanol extract, 4-9 parts of radix peucedani enzymatic hydrolysate, 6-11 parts of acanthopanax enzymatic hydrolysate and 6-15 parts of an achyranthes bidentata water-extraction and alcohol-precipitation extract. The preparation method comprises such processes as water extraction, enzymatic hydrolysis, alcohol precipitation, stirring and condensing. The prepared traditional Chinese medicine disclosed by the invention has a remarkable effect on treating gout, and is capable of effectively curing gout symptoms of patients.
Description
Technical field
The invention belongs to the field of Chinese medicines, it is related to a kind of Chinese medical extract and preparation method thereof, more particularly to a kind for the treatment of
Chinese medical extract of gout and preparation method thereof.
Background technology
It is the key reason that gout occurs that uric acid in blood increases for a long time.Uric Acid is mainly derived from two aspects: one
It is the nucleic acid and other purine compound that human body cell internal protein catabolism produces, generate through the effect of some enzymes interior
Source property uric acid.Two is contained purine compound in food, nucleic acid and nucleoprotein composition, after digestion with absorption, through one
The effect of a little enzymes generates exogenous uric acid.Gout precisely due to various factors leads to the activity of these enzymes abnormal, for example, promotes urine
The increased activity of acid enzyme, reduced activity of suppression uric acid synzyme etc., thus it is excessive to lead to uric acid to generate, make uric acid in blood
Build up in liquid, produce hyperuricemia.
Patient with gout can treat gout using medicine.Wherein Chinese medicine is a preferably selection, patent application publication
Disclosure of the invention number for cn103933224 to treat gout using the red sage root, giant knotweed, corydalis tuber, the composition such as windproof, but it is simultaneously
Active ingredient therein is not rationally screened, therefore while treating gout, also created a lot of side effects.This
Force us to need the active ingredient in Chinese medicine is screened, to obtain the Chinese medical extract for the treatment of gout.
Content of the invention
Technical problem to be solved: the DeGrain of conventional treatment by Chinese herbs gout it is impossible to effectively cure patient by
The arthralgia that produces in gout, swelling, the symptom such as stiff are it is therefore desirable to a kind of traditional Chinese medicine extraction that can effectively treat gout
Thing and preparation method thereof.
Technical scheme: for the problems referred to above, the invention discloses a kind of Chinese medical extract treating gout, described treatment
The Chinese medical extract of gout includes the extract of following weight parts: Radix Angelicae Sinensis water extract is 4～10 parts, pseudo-ginseng water extract is 7～13
Part, corydalis tuber ethanol extract are 5～10 parts, root of purple-flowered peucedanum zymolyte is 4～9 parts.
Preferably, described a kind of Chinese medical extract treating gout, including the extract of following weight parts: Radix Angelicae Sinensis water extraction
Thing is 4～10 parts, pseudo-ginseng water extract is 7～13 parts, corydalis tuber ethanol extract is 5～10 parts, root of purple-flowered peucedanum zymolyte is 4～9 parts, thorn
Slender acanthopanax zymolyte is 6～11 parts, root of bidentate achyranthes water extracting alcohol hypostasis is 6～15 parts.
It is further preferred that a kind of described Chinese medical extract treating gout, including the extract of following weight parts: when
Return water extract to be 6 parts, pseudo-ginseng water extract be 10 parts, corydalis tuber ethanol extract be 8 parts, root of purple-flowered peucedanum zymolyte be 7 parts, wilsonii enzymolysis
Thing is 8 parts, root of bidentate achyranthes water extracting alcohol hypostasis is 11 parts.
A kind of preparation method of the Chinese medical extract treating gout, the preparation side of the described Chinese medical extract for the treatment of gout
Method is as follows: (1) is extracted with water after pulverizing Gansu Angelica, then extract is separated with residue, by supernatant after separating
Concentrated, after concentration, obtained Radix Angelicae Sinensis water extract；(2) after complete pseudo-ginseng being plucked, pseudo-ginseng is carried out freeze-drying, after being dried
Grind, extracted with water, centrifugation after extraction, collect supernatant, concentrated supernatant obtains pseudo-ginseng water extract；(3) by unit
Recklessly smash to pieces, add ethanol to be extracted, after extraction, extract is filtered, then obtain corydalis tuber ethanol extract after filtrate is concentrated；
(4) root of purple-flowered peucedanum is dried, after being dried, the root of purple-flowered peucedanum is pulverized, in the root of purple-flowered peucedanum, add water and cellulase, after enzymolysis, take upper strata to digest
Liquid, obtains root of purple-flowered peucedanum zymolyte after enzymolysis liquid is concentrated；(5) wilsonii is pulverized, add cellulase and water after pulverizing, carry out enzyme
Solution, then concentrate after the collection of upper strata enzymolysis liquid, obtain wilsonii zymolyte；(6) root of bidentate achyranthes is shredded, carried with water after shredding
Take, filter after extraction, ethanol will be added in filtrate to carry out alcohol precipitation, collect precipitation, be root of bidentate achyranthes water extracting alcohol hypostasis；(7) take by weight
Radix Angelicae Sinensis water extract is 6 parts, pseudo-ginseng water extract is 10 parts, corydalis tuber ethanol extract is 8 parts, root of purple-flowered peucedanum zymolyte is 7 parts, wilsonii enzyme
Solution thing is 8 parts, root of bidentate achyranthes water extracting alcohol hypostasis is 11 parts, is the Chinese medical extract for the treatment of gout after above-mentioned each composition is mixed.
Preferably, the preparation method of described a kind of Chinese medical extract treating gout, Radix Angelicae Sinensis water extract in preparation method
Preparation process is as follows: Gansu Angelica is pulverized first, is crushed to when being classified as 200 mesh, is extracted with water, solid-liquid ratio after pulverizing
For 1:6, Extracting temperature is 45 DEG C, and extraction time is 3h, then by extract and residue centrifugation, after separating carries out supernatant
It is concentrated by evaporation, after concentration, obtain Radix Angelicae Sinensis water extract.
Preferably, the preparation method of described a kind of Chinese medical extract treating gout, pseudo-ginseng water extract in preparation method
Preparation process is as follows: after complete pseudo-ginseng is plucked, pseudo-ginseng is carried out freeze-drying, freeze-drying temperature is -25 DEG C, when being dried
Between be 6h, after being dried, pseudo-ginseng is ground, is extracted with water, solid-liquid ratio is 1:4, Extracting temperature is 50 DEG C, and extraction time is
120min, centrifugation after extraction, collect supernatant, concentrated supernatant obtains pseudo-ginseng water extract.
Preferably, the preparation method of described a kind of Chinese medical extract treating gout, in preparation method, corydalis tuber ethanol carries
Take thing preparation process as follows: corydalis tuber to be smashed to pieces, adds ethanol to be stirred extracting, alcohol extract temperature is 60 DEG C, extraction time
For 90min, ethanol weight is 4 times of corydalis tuber weight, after extraction filters extract, then obtains corydalis tuber ethanol after filtrate is concentrated
Extract.
Preferably, the preparation method of described a kind of Chinese medical extract treating gout, root of purple-flowered peucedanum zymolyte in preparation method
Preparation process is as follows: the root of purple-flowered peucedanum is dried, after being dried, the root of purple-flowered peucedanum pulverized, add the aqueous solution of cellulase in the root of purple-flowered peucedanum, fine
The mass fraction of the plain enzyme of dimension is 1wt%, and aqueous solution weight is 5 times of root of purple-flowered peucedanum weight, carries out stirring enzymolysis, enzymolysis time under room temperature
For 2h, after enzymolysis, take upper strata enzymolysis liquid, after enzymolysis liquid is concentrated, obtain root of purple-flowered peucedanum zymolyte.
Preferably, the preparation method of described a kind of Chinese medical extract treating gout, wilsonii enzymolysis in preparation method
The preparation process of thing is as follows: wilsonii is pulverized, adds the cellulase aqueous solution after pulverizing, the concentration of cellulase is
1.5wt%, solid-liquid ratio is 1:3, carries out stirring enzymolysis under room temperature, then concentrates after the collection of upper strata enzymolysis liquid, and concentrate is wilsonii
Zymolyte.
Preferably, the preparation method of described a kind of Chinese medical extract treating gout, root of bidentate achyranthes water extracting alcohol in preparation method
The preparation process of hypostasis is as follows: the root of bidentate achyranthes is shredded, after shredding is extracted the root of bidentate achyranthes with water, solid-liquid ratio is 1:3, Extracting temperature is
Room temperature, extraction time is 3h, uses filter-cloth filtering after extraction, adds ethanol to carry out alcohol precipitation in filtrate, and ethanol addition is filtrate
4 times of weight, collect precipitation, are root of bidentate achyranthes water extracting alcohol hypostasis after precipitation is dried.
In terms for the treatment of gout, Radix Angelicae Sinensis and pseudo-ginseng have certain curative effect.But the Chinese medicine preparation of the treatment gout of routine
Majority to obtain active ingredient by way of decocting, and the present invention is extracted to it, obtains treatment gout therein
Active ingredient.In addition, it is also added into zymolyte and the water extracting alcohol hypostasis of some other Chinese medicines, obtain treatment gout
Good result.
Beneficial effect: the Chinese medical extract of the present invention has the curative effect of very good treatment gout.Radix Angelicae Sinensis water extract is 4
～10 parts, pseudo-ginseng water extract be 7～13 parts, corydalis tuber ethanol extract be 5～10 parts, root of purple-flowered peucedanum zymolyte be 4～9 parts, wilsonii
When zymolyte is 6～11 parts, root of bidentate achyranthes water extracting alcohol hypostasis is 6～15 parts, the Chinese medical extract treatment gout effect of acquisition preferably, can
To be effectively used for treating the gout symptom of patient.
Specific embodiment
Embodiment 1
(1) Gansu Angelica is pulverized first, be crushed to when being classified as 200 mesh, extracted with water after pulverizing, solid-liquid ratio is
1:6, Extracting temperature is 45 DEG C, and extraction time is 3h, then by extract and residue centrifugation, after separating is steamed supernatant
Send out and concentrate, after concentration, obtain Radix Angelicae Sinensis water extract；(2), after complete pseudo-ginseng being plucked, pseudo-ginseng is carried out freeze-drying, freeze-drying
Temperature is -25 DEG C, and drying time is 6h, grinds pseudo-ginseng, extracted with water after being dried, and solid-liquid ratio is 1:4, and Extracting temperature is
50 DEG C, extraction time is 120min, and supernatant is collected in centrifugation after extraction, and concentrated supernatant obtains pseudo-ginseng water extract；(3)
Corydalis tuber is smashed to pieces, adds ethanol to be stirred extracting, alcohol extract temperature is 60 DEG C, extraction time is 90min, ethanol weight is
Extract is filtered after extraction by 4 times of corydalis tuber weight, then obtains corydalis tuber ethanol extract after filtrate is concentrated；(4) root of purple-flowered peucedanum is entered
The root of purple-flowered peucedanum is pulverized after being dried by row drying, adds the aqueous solution of cellulase in the root of purple-flowered peucedanum, and the mass fraction of cellulase is
1wt%, aqueous solution weight is 5 times of root of purple-flowered peucedanum weight, carries out stirring enzymolysis under room temperature, and enzymolysis time is 2h, takes upper strata enzyme after enzymolysis
Solution liquid, obtains root of purple-flowered peucedanum zymolyte after enzymolysis liquid is concentrated；(5) wilsonii is pulverized, after pulverizing, add the cellulase aqueous solution, fine
The concentration of dimension element enzyme is 1.5wt%, and solid-liquid ratio is 1:3, carries out stirring enzymolysis under room temperature, then concentrates after the collection of upper strata enzymolysis liquid,
Concentrate is wilsonii zymolyte；(6) root of bidentate achyranthes is shredded, after shredding, the root of bidentate achyranthes is extracted with water, solid-liquid ratio is 1:3, extracts
Temperature is room temperature, and extraction time is 3h, uses filter-cloth filtering after extraction, adds ethanol to carry out alcohol precipitation, ethanol addition in filtrate
For 4 times of filtrate weight, collect precipitation, be root of bidentate achyranthes water extracting alcohol hypostasis after precipitation is dried；(7) the Radix Angelicae Sinensis water extract is taken to be by weight
6 parts, pseudo-ginseng water extract be 10 parts, corydalis tuber ethanol extract be 8 parts, root of purple-flowered peucedanum zymolyte be 7 parts, wilsonii zymolyte be 8 parts, ox
Knee water extracting alcohol hypostasis is 11 parts, is the Chinese medical extract for the treatment of gout after above-mentioned each composition is mixed.
Embodiment 2
(1) Gansu Angelica is pulverized first, be crushed to when being classified as 200 mesh, extracted with water after pulverizing, solid-liquid ratio is
1:6, Extracting temperature is 45 DEG C, and extraction time is 3h, then by extract and residue centrifugation, after separating is steamed supernatant
Send out and concentrate, after concentration, obtain Radix Angelicae Sinensis water extract；(2), after complete pseudo-ginseng being plucked, pseudo-ginseng is carried out freeze-drying, freeze-drying
Temperature is -25 DEG C, and drying time is 6h, grinds pseudo-ginseng, extracted with water after being dried, and solid-liquid ratio is 1:4, and Extracting temperature is
50 DEG C, extraction time is 120min, and supernatant is collected in centrifugation after extraction, and concentrated supernatant obtains pseudo-ginseng water extract；(3)
Corydalis tuber is smashed to pieces, adds ethanol to be stirred extracting, alcohol extract temperature is 60 DEG C, extraction time is 90min, ethanol weight is
Extract is filtered after extraction by 4 times of corydalis tuber weight, then obtains corydalis tuber ethanol extract after filtrate is concentrated；(4) root of purple-flowered peucedanum is entered
The root of purple-flowered peucedanum is pulverized after being dried by row drying, adds the aqueous solution of cellulase in the root of purple-flowered peucedanum, and the mass fraction of cellulase is
1wt%, aqueous solution weight is 5 times of root of purple-flowered peucedanum weight, carries out stirring enzymolysis under room temperature, and enzymolysis time is 2h, takes upper strata enzyme after enzymolysis
Solution liquid, obtains root of purple-flowered peucedanum zymolyte after enzymolysis liquid is concentrated；(5) Radix Angelicae Sinensis water extract is taken to be 4 parts by weight, pseudo-ginseng water extract is 13
Part, corydalis tuber ethanol extract are 5 parts, root of purple-flowered peucedanum zymolyte is 9 parts, are the Chinese medicine for the treatment of gout after above-mentioned each composition is mixed
Extract.
Embodiment 3
(1) Gansu Angelica is pulverized first, be crushed to when being classified as 200 mesh, extracted with water after pulverizing, solid-liquid ratio is
1:6, Extracting temperature is 45 DEG C, and extraction time is 3h, then by extract and residue centrifugation, after separating is steamed supernatant
Send out and concentrate, after concentration, obtain Radix Angelicae Sinensis water extract；(2), after complete pseudo-ginseng being plucked, pseudo-ginseng is carried out freeze-drying, freeze-drying
Temperature is -25 DEG C, and drying time is 6h, grinds pseudo-ginseng, extracted with water after being dried, and solid-liquid ratio is 1:4, and Extracting temperature is
50 DEG C, extraction time is 120min, and supernatant is collected in centrifugation after extraction, and concentrated supernatant obtains pseudo-ginseng water extract；(3)
Corydalis tuber is smashed to pieces, adds ethanol to be stirred extracting, alcohol extract temperature is 60 DEG C, extraction time is 90min, ethanol weight is
Extract is filtered after extraction by 4 times of corydalis tuber weight, then obtains corydalis tuber ethanol extract after filtrate is concentrated；(4) root of purple-flowered peucedanum is entered
The root of purple-flowered peucedanum is pulverized after being dried by row drying, adds the aqueous solution of cellulase in the root of purple-flowered peucedanum, and the mass fraction of cellulase is
1wt%, aqueous solution weight is 5 times of root of purple-flowered peucedanum weight, carries out stirring enzymolysis under room temperature, and enzymolysis time is 2h, takes upper strata enzyme after enzymolysis
Solution liquid, obtains root of purple-flowered peucedanum zymolyte after enzymolysis liquid is concentrated；(5) Radix Angelicae Sinensis water extract is taken to be 10 parts by weight, pseudo-ginseng water extract is 7
Part, corydalis tuber ethanol extract are 10 parts, root of purple-flowered peucedanum zymolyte is 4 parts, are in treatment gout after above-mentioned each composition is mixed
Medicament extract.
Embodiment 4
(1) Gansu Angelica is pulverized first, be crushed to when being classified as 200 mesh, extracted with water after pulverizing, solid-liquid ratio is
1:6, Extracting temperature is 45 DEG C, and extraction time is 3h, then by extract and residue centrifugation, after separating is steamed supernatant
Send out and concentrate, after concentration, obtain Radix Angelicae Sinensis water extract；(2), after complete pseudo-ginseng being plucked, pseudo-ginseng is carried out freeze-drying, freeze-drying
Temperature is -25 DEG C, and drying time is 6h, grinds pseudo-ginseng, extracted with water after being dried, and solid-liquid ratio is 1:4, and Extracting temperature is
50 DEG C, extraction time is 120min, and supernatant is collected in centrifugation after extraction, and concentrated supernatant obtains pseudo-ginseng water extract；(3)
Corydalis tuber is smashed to pieces, adds ethanol to be stirred extracting, alcohol extract temperature is 60 DEG C, extraction time is 90min, ethanol weight is
Extract is filtered after extraction by 4 times of corydalis tuber weight, then obtains corydalis tuber ethanol extract after filtrate is concentrated；(4) root of purple-flowered peucedanum is entered
The root of purple-flowered peucedanum is pulverized after being dried by row drying, adds the aqueous solution of cellulase in the root of purple-flowered peucedanum, and the mass fraction of cellulase is
1wt%, aqueous solution weight is 5 times of root of purple-flowered peucedanum weight, carries out stirring enzymolysis under room temperature, and enzymolysis time is 2h, takes upper strata enzyme after enzymolysis
Solution liquid, obtains root of purple-flowered peucedanum zymolyte after enzymolysis liquid is concentrated；(5) wilsonii is pulverized, after pulverizing, add the cellulase aqueous solution, fine
The concentration of dimension element enzyme is 1.5wt%, and solid-liquid ratio is 1:3, carries out stirring enzymolysis under room temperature, then concentrates after the collection of upper strata enzymolysis liquid,
Concentrate is wilsonii zymolyte；(6) root of bidentate achyranthes is shredded, after shredding, the root of bidentate achyranthes is extracted with water, solid-liquid ratio is 1:3, extracts
Temperature is room temperature, and extraction time is 3h, uses filter-cloth filtering after extraction, adds ethanol to carry out alcohol precipitation, ethanol addition in filtrate
For 4 times of filtrate weight, collect precipitation, be root of bidentate achyranthes water extracting alcohol hypostasis after precipitation is dried；(7) the Radix Angelicae Sinensis water extract is taken to be by weight
4 parts, pseudo-ginseng water extract be 13 parts, corydalis tuber ethanol extract be 5 parts, root of purple-flowered peucedanum zymolyte be 9 parts, wilsonii zymolyte be 6 parts, ox
Knee water extracting alcohol hypostasis is 15 parts, is the Chinese medical extract for the treatment of gout after above-mentioned each composition is mixed.
Embodiment 5
(1) Gansu Angelica is pulverized first, be crushed to when being classified as 200 mesh, extracted with water after pulverizing, solid-liquid ratio is
1:6, Extracting temperature is 45 DEG C, and extraction time is 3h, then by extract and residue centrifugation, after separating is steamed supernatant
Send out and concentrate, after concentration, obtain Radix Angelicae Sinensis water extract；(2), after complete pseudo-ginseng being plucked, pseudo-ginseng is carried out freeze-drying, freeze-drying
Temperature is -25 DEG C, and drying time is 6h, grinds pseudo-ginseng, extracted with water after being dried, and solid-liquid ratio is 1:4, and Extracting temperature is
50 DEG C, extraction time is 120min, and supernatant is collected in centrifugation after extraction, and concentrated supernatant obtains pseudo-ginseng water extract；(3)
Corydalis tuber is smashed to pieces, adds ethanol to be stirred extracting, alcohol extract temperature is 60 DEG C, extraction time is 90min, ethanol weight is
Extract is filtered after extraction by 4 times of corydalis tuber weight, then obtains corydalis tuber ethanol extract after filtrate is concentrated；(4) root of purple-flowered peucedanum is entered
The root of purple-flowered peucedanum is pulverized after being dried by row drying, adds the aqueous solution of cellulase in the root of purple-flowered peucedanum, and the mass fraction of cellulase is
1wt%, aqueous solution weight is 5 times of root of purple-flowered peucedanum weight, carries out stirring enzymolysis under room temperature, and enzymolysis time is 2h, takes upper strata enzyme after enzymolysis
Solution liquid, obtains root of purple-flowered peucedanum zymolyte after enzymolysis liquid is concentrated；(5) wilsonii is pulverized, after pulverizing, add the cellulase aqueous solution, fine
The concentration of dimension element enzyme is 1.5wt%, and solid-liquid ratio is 1:3, carries out stirring enzymolysis under room temperature, then concentrates after the collection of upper strata enzymolysis liquid,
Concentrate is wilsonii zymolyte；(6) root of bidentate achyranthes is shredded, after shredding, the root of bidentate achyranthes is extracted with water, solid-liquid ratio is 1:3, extracts
Temperature is room temperature, and extraction time is 3h, uses filter-cloth filtering after extraction, adds ethanol to carry out alcohol precipitation, ethanol addition in filtrate
For 4 times of filtrate weight, collect precipitation, be root of bidentate achyranthes water extracting alcohol hypostasis after precipitation is dried；(7) the Radix Angelicae Sinensis water extract is taken to be by weight
10 parts, pseudo-ginseng water extract be 7 parts, corydalis tuber ethanol extract be 10 parts, root of purple-flowered peucedanum zymolyte be 4 parts, wilsonii zymolyte be 11 parts,
Root of bidentate achyranthes water extracting alcohol hypostasis is 6 parts, is the Chinese medical extract for the treatment of gout after above-mentioned each composition is mixed.
The patient of arthragra symptom is divided into 5 groups, each group of people's number is 10 people, the Chinese medicine taking embodiment 1 to 5 respectively carries
Take thing, take once a day, each take 2g, the cycle of taking is 20 days.The gout treatment situation of patient with gout is observed after 20 days.
After the Chinese medical extract having taken embodiment 1, the arthroncus of 8 people, pain, stiff symptom are shown 10 people
The healing writing, the arthroncus of 2 people, pain, stiff symptom are greatly alleviated.
After the Chinese medical extract having taken embodiment 2, the arthroncus of 6 people, pain, stiff symptom obtain pole to 10 people
Big alleviation, the arthroncus of 4 people, pain, stiff symptom are effectively alleviated.
After the Chinese medical extract having taken embodiment 3, the arthroncus of 7 people, pain, stiff symptom obtain pole to 10 people
Big alleviation, the arthroncus of 3 people, pain, stiff symptom are effectively alleviated.
After the Chinese medical extract having taken embodiment 4, the arthroncus of 3 people, pain, stiff symptom are shown 10 people
The healing writing, the arthroncus of 7 people, pain, stiff symptom are greatly alleviated.
After the Chinese medical extract having taken embodiment 5, the arthroncus of 5 people, pain, stiff symptom are shown 10 people
The healing writing, the arthroncus of 5 people, pain, stiff symptom are greatly alleviated.
From the point of view of the effect for the treatment of patient's gout, the therapeutic effect of embodiment 1 is optimal, the treatment of embodiment 4 and embodiment 5
Effect is also very good, and the therapeutic effect of embodiment 2 and 3 is preferable.
Contrast experiment: contrast experiment adopt Chinese medicine be the red sage root be 5 weight portions, corydalis tuber be 8 weight portions, windproof be 3 weights
Amount part.Chinese medicine extract is obtained after decocting.Assay method with embodiment 1 to 5, result is: 10 people are in having taken contrast experiment
After Chinese medicine extract, the arthroncus of 3 people, pain, stiff symptom are significantly cured, the arthroncus of 7 people, pain,
Stiff symptom is greatly alleviated.
Claims (7)
1. a kind of Chinese medical extract treating gout it is characterised in that the Chinese medical extract of described treatment gout include following heavy
The extract of amount part: Radix Angelicae Sinensis water extract is 6 parts, pseudo-ginseng water extract is 10 parts, corydalis tuber ethanol extract is 8 parts, root of purple-flowered peucedanum zymolyte
For 7 parts, wilsonii zymolyte be 8 parts, root of bidentate achyranthes water extracting alcohol hypostasis be 11 parts；
A kind of described Chinese medical extract treating gout is prepared by following preparation methods: (1) is by Gansu Angelica powder
Extracted with water after broken, then extract is separated with residue, after separating, supernatant is concentrated, after concentration, obtain Radix Angelicae Sinensis water
Extract；(2) after complete pseudo-ginseng being plucked, pseudo-ginseng is carried out freeze-drying, grind after being dried, extracted with water, after extraction
Centrifugation, collects supernatant, and concentrated supernatant obtains pseudo-ginseng water extract；(3) corydalis tuber is smashed to pieces, adds ethanol to be extracted,
After extraction, extract is filtered, then obtain corydalis tuber ethanol extract after filtrate is concentrated；(4) root of purple-flowered peucedanum is dried, after being dried
The root of purple-flowered peucedanum is pulverized, adds water and cellulase in the root of purple-flowered peucedanum, take upper strata enzymolysis liquid after enzymolysis, after enzymolysis liquid is concentrated, obtain the root of purple-flowered peucedanum
Zymolyte；(5) wilsonii is pulverized, add cellulase and water after pulverizing, digested, then will be dense after the collection of upper strata enzymolysis liquid
Contracting, obtains wilsonii zymolyte；(6) root of bidentate achyranthes is shredded, extracted with water after shredding, filter after extraction, will add in filtrate
Ethanol carries out alcohol precipitation, collects precipitation, is root of bidentate achyranthes water extracting alcohol hypostasis；(7) Radix Angelicae Sinensis water extract is taken to be 6 parts, pseudo-ginseng water extract by weight
For 10 parts, corydalis tuber ethanol extract be 8 parts, root of purple-flowered peucedanum zymolyte be 7 parts, wilsonii zymolyte be 8 parts, root of bidentate achyranthes water extracting alcohol hypostasis be
11 parts, be the Chinese medical extract for the treatment of gout after above-mentioned each composition is mixed.
2. a kind of Chinese medical extract treating gout according to claim 1 is it is characterised in that Radix Angelicae Sinensis water in preparation method
Extract preparation process is as follows: Gansu Angelica pulverized first, is crushed to when being classified as 200 mesh, extracted with water after pulverizing, material
Liquor ratio is 1:6, and Extracting temperature is 45 DEG C, and extraction time is 3h, then by extract and residue centrifugation, by supernatant after separating
It is evaporated concentrating, after concentration, obtain Radix Angelicae Sinensis water extract.
3. a kind of Chinese medical extract treating gout according to claim 1 is it is characterised in that pseudo-ginseng water in preparation method
Extract preparation process is as follows: after complete pseudo-ginseng is plucked, pseudo-ginseng is carried out freeze-drying, freeze-drying temperature is -25 DEG C, does
The dry time is 6h, grinds pseudo-ginseng, extracted with water after being dried, and solid-liquid ratio is 1:4, and Extracting temperature is 50 DEG C, extraction time
For 120min, centrifugation after extraction, collect supernatant, concentrated supernatant obtains pseudo-ginseng water extract.
4. a kind of Chinese medical extract treating gout according to claim 1 is it is characterised in that corydalis tuber second in preparation method
Alcohol extracting thing preparation process is as follows: corydalis tuber is smashed to pieces, adds ethanol to be stirred extracting, alcohol extract temperature is 60 DEG C, extracts
Time is 90min, and ethanol weight is 4 times of corydalis tuber weight, after extraction filters extract, then obtains corydalis tuber after filtrate is concentrated
Ethanol extract.
5. a kind of Chinese medical extract treating gout according to claim 1 is it is characterised in that root of purple-flowered peucedanum enzyme in preparation method
Solution thing preparation process is as follows: the root of purple-flowered peucedanum is dried, after being dried pulverizes the root of purple-flowered peucedanum, add the water-soluble of cellulase in the root of purple-flowered peucedanum
Liquid, the mass fraction of cellulase is 1wt%, and aqueous solution weight is 5 times of root of purple-flowered peucedanum weight, carries out stirring enzymolysis under room temperature, enzymolysis
Time is 2h, takes upper strata enzymolysis liquid, obtain root of purple-flowered peucedanum zymolyte after enzymolysis liquid is concentrated after enzymolysis.
6. a kind of Chinese medical extract treating gout according to claim 1 is it is characterised in that wilsonii in preparation method
The preparation process of zymolyte is as follows: wilsonii is pulverized, adds the cellulase aqueous solution after pulverizing, the concentration of cellulase is
1.5wt%, solid-liquid ratio is 1:3, carries out stirring enzymolysis under room temperature, then concentrates after the collection of upper strata enzymolysis liquid, and concentrate is wilsonii
Zymolyte.
7. a kind of Chinese medical extract treating gout according to claim 1 is it is characterised in that root of bidentate achyranthes water in preparation method
The preparation process carrying alcohol hypostasis is as follows: the root of bidentate achyranthes is shredded, after shredding is extracted the root of bidentate achyranthes with water, solid-liquid ratio is 1:3, extracts temperature
Spend for room temperature, extraction time is 3h, uses filter-cloth filtering after extraction, add ethanol to carry out alcohol precipitation in filtrate, ethanol addition is
4 times of filtrate weight, collect precipitation, are root of bidentate achyranthes water extracting alcohol hypostasis after precipitation is dried.
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