CN104274538A - Traditional Chinese medicine extract for treating gout and preparation method thereof - Google Patents
Traditional Chinese medicine extract for treating gout and preparation method thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/21—Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/254—Acanthopanax or Eleutherococcus
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/66—Papaveraceae (Poppy family), e.g. bloodroot
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention belongs to the field of a traditional Chinese medicine, and discloses a traditional Chinese medicine extract for treating gout and a preparation method thereof. The traditional Chinese medicine extract includes the following extracts in parts by weight: 4-10 parts of an angelica aqueous extract, 7-13 parts of a pseudo-ginseng aqueous extract, 5-10 parts of a rhizoma corydalis ethanol extract, 4-9 parts of radix peucedani enzymatic hydrolysate, 6-11 parts of acanthopanax enzymatic hydrolysate and 6-15 parts of an achyranthes bidentata water-extraction and alcohol-precipitation extract. The preparation method comprises such processes as water extraction, enzymatic hydrolysis, alcohol precipitation, stirring and condensing. The prepared traditional Chinese medicine disclosed by the invention has a remarkable effect on treating gout, and is capable of effectively curing gout symptoms of patients.
Description
Technical field
The invention belongs to the field of Chinese medicines, relate to a kind of Chinese medicine extract and preparation method thereof, particularly relate to a kind of Chinese medicine extract for the treatment of gout and preparation method thereof.
Background technology
It is the key reason that gout occurs that uric acid in blood increases for a long time.Uric Acid is mainly derived from two aspects: one be human body cell internal protein catabolism produce nucleic acid and other purine compound, through some enzymes effect and generate endogenous uric acid.Two is purine compound, nucleic acid and nucleoprotein compositions contained in food, and after peptic ulcer bleeding, the effect through some enzymes generates exogenous uric acid.Gout is exactly because various factors causes the activity of these enzymes abnormal, such as, promote the increased activity of uric acid synzyme, suppress the reduced activity etc. of uric acid synzyme, thus cause uricopoiesis too much, uric acid is built up in blood, produces hyperuricemia.
Patient with gout can adopt medicine to treat gout.Wherein Chinese medicine is a selection preferably, public announcement of a patent application number has been the disclosure of the invention of CN103933224, and employing Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati, Rhizoma Corydalis, Radix Saposhnikoviae etc. become to assign to treat gout, but it does not rationally screen effective ingredient wherein, therefore while treatment gout, also create a lot of side effect.This also forces us to need to screen the effective ingredient in Chinese medicine, obtains the Chinese medicine extract for the treatment of gout.
Summary of the invention
The technical problem solved: the DeGrain of conventional treatment by Chinese herbs gout, effectively can not cure arthralgia that patient produces due to gout, swelling, the symptom such as stiff, therefore need a kind of can effective Chinese medicine extract for the treatment of gout and preparation method thereof.
Technical scheme: for the problems referred to above, the invention discloses a kind of Chinese medicine extract for the treatment of gout, the Chinese medicine extract of described treatment gout comprises the extract of following weight parts: Radix Angelicae Sinensis water extract is 4 ~ 10 parts, pseudo-ginseng water extract is 7 ~ 13 parts, Rhizoma Corydalis ethanol extraction is 5 ~ 10 parts, Radix Peucedani zymolyte is 4 ~ 9 parts.
Preferably, described a kind of Chinese medicine extract for the treatment of gout, comprises the extract of following weight parts: Radix Angelicae Sinensis water extract is 4 ~ 10 parts, pseudo-ginseng water extract is 7 ~ 13 parts, Rhizoma Corydalis ethanol extraction is 5 ~ 10 parts, Radix Peucedani zymolyte is 4 ~ 9 parts, Radix Et Caulis Acanthopanacis Senticosi zymolyte is 6 ~ 11 parts, Radix Achyranthis Bidentatae water extracting alcohol hypostasis is 6 ~ 15 parts.
Preferred further, described a kind of Chinese medicine extract for the treatment of gout, comprises the extract of following weight parts: Radix Angelicae Sinensis water extract is 6 parts, pseudo-ginseng water extract is 10 parts, Rhizoma Corydalis ethanol extraction is 8 parts, Radix Peucedani zymolyte is 7 parts, Radix Et Caulis Acanthopanacis Senticosi zymolyte is 8 parts, Radix Achyranthis Bidentatae water extracting alcohol hypostasis is 11 parts.
A kind of preparation method for the treatment of the Chinese medicine extract of gout, the preparation method of the Chinese medicine extract of described treatment gout is as follows: (1) extracts with water after Gansu Angelica being pulverized, again extracting solution is separated with residue, after separation, supernatant is concentrated, after concentrated, obtain Radix Angelicae Sinensis water extract; (2) after being plucked by complete Radix Notoginseng, Radix Notoginseng is carried out lyophilization, grinds after drying, extract with water, centrifugalize after extracting, collect supernatant, concentrated supernatant obtains pseudo-ginseng water extract; (3) Rhizoma Corydalis is smashed to pieces, add ethanol and extract, by extracting liquid filtering after extraction, then obtain Rhizoma Corydalis ethanol extraction after filtrate being concentrated; (4) Radix Peucedani is carried out drying, after drying, Radix Peucedani is pulverized, in Radix Peucedani, add water and cellulase, after enzymolysis, get upper strata enzymolysis solution, after enzymolysis solution is concentrated, obtain Radix Peucedani zymolyte; (5) Radix Et Caulis Acanthopanacis Senticosi is pulverized, add cellulase and water after pulverizing, carry out enzymolysis, more concentrated after upper strata enzymolysis solution is collected, obtain Radix Et Caulis Acanthopanacis Senticosi zymolyte; (6) shredded by Radix Achyranthis Bidentatae, shred rear water and extract, filter after extracting, carrying out precipitate with ethanol, collecting precipitation by adding ethanol in filtrate, is Radix Achyranthis Bidentatae water extracting alcohol hypostasis; (7) getting that Radix Angelicae Sinensis water extract is 6 parts, pseudo-ginseng water extract is 10 parts, Rhizoma Corydalis ethanol extraction is 8 parts, Radix Peucedani zymolyte is 7 parts, Radix Et Caulis Acanthopanacis Senticosi zymolyte is 8 parts, Radix Achyranthis Bidentatae water extracting alcohol hypostasis is 11 parts by weight, will be the Chinese medicine extract for the treatment of gout after above-mentioned each composition mix homogeneously.
Preferably, described a kind of preparation method for the treatment of the Chinese medicine extract of gout, in preparation method, Radix Angelicae Sinensis water extract preparation process is as follows: Gansu Angelica first pulverized, and being crushed to when being classified as 200 orders, extracting after pulverizing with water, solid-liquid ratio is 1:6, Extracting temperature is 45 DEG C, and extraction time is 3h, then by extracting solution and residue centrifugalize, after separation, supernatant is carried out evaporation and concentration, after concentrated, obtain Radix Angelicae Sinensis water extract.
Preferably, described a kind of preparation method for the treatment of the Chinese medicine extract of gout, in preparation method, pseudo-ginseng water extract preparation process is as follows: after being plucked by complete Radix Notoginseng, Radix Notoginseng is carried out lyophilization, and lyophilization temperature is-25 DEG C, and drying time is 6h, after drying, Radix Notoginseng is ground, extract with water, solid-liquid ratio is 1:4, and Extracting temperature is 50 DEG C, extraction time is 120min, centrifugalize after extracting, collect supernatant, concentrated supernatant obtains pseudo-ginseng water extract.
Preferably, described a kind of preparation method for the treatment of the Chinese medicine extract of gout, in preparation method, Rhizoma Corydalis ethanol extraction preparation process is as follows: smashed to pieces by Rhizoma Corydalis, add ethanol and carry out stirring extraction, ethanol extraction temperature is 60 DEG C, and extraction time is 90min, and ethanol weight is 4 times of Rhizoma Corydalis weight, by extracting liquid filtering after extraction, then obtain Rhizoma Corydalis ethanol extraction after filtrate being concentrated.
Preferably, described a kind of preparation method for the treatment of the Chinese medicine extract of gout, in preparation method, Radix Peucedani zymolyte preparation process is as follows: Radix Peucedani is carried out drying, is pulverized by Radix Peucedani, add the aqueous solution of cellulase in Radix Peucedani after drying, the mass fraction of cellulase is 1wt%, aqueous solution weight is 5 times of Radix Peucedani weight, carries out stirred at ambient temperature enzymolysis, and enzymolysis time is 2h, get upper strata enzymolysis solution after enzymolysis, after enzymolysis solution is concentrated, obtain Radix Peucedani zymolyte.
Preferably, described a kind of preparation method for the treatment of the Chinese medicine extract of gout, in preparation method, the preparation process of Radix Et Caulis Acanthopanacis Senticosi zymolyte is as follows: pulverized by Radix Et Caulis Acanthopanacis Senticosi, cellulase aqueous solution is added after pulverizing, the concentration of cellulase is 1.5wt%, and solid-liquid ratio is 1:3, carries out stirred at ambient temperature enzymolysis, concentrated after being collected by upper strata enzymolysis solution, concentrate is Radix Et Caulis Acanthopanacis Senticosi zymolyte again.
Preferably, described a kind of preparation method for the treatment of the Chinese medicine extract of gout, in preparation method, the preparation process of Radix Achyranthis Bidentatae water extracting alcohol hypostasis is as follows: shredded by Radix Achyranthis Bidentatae, is extracted by Radix Achyranthis Bidentatae water after shredding, solid-liquid ratio is 1:3, Extracting temperature is room temperature, and extraction time is 3h, extracts rear filter-cloth filtering, in filtrate, add ethanol carry out precipitate with ethanol, ethanol addition is 4 times of filtrate weight, collecting precipitation, is Radix Achyranthis Bidentatae water extracting alcohol hypostasis after precipitating drying.
In treatment gout, Radix Angelicae Sinensis and Radix Notoginseng have certain curative effect.But the Chinese medicine formula of the treatment gout of routine majority have passed the mode that decocts to obtain effective ingredient, and the present invention extracts it, obtains the effective ingredient for the treatment of gout wherein.In addition, also add zymolyte and the water extracting alcohol hypostasis of some other Chinese medicines, obtain the good result for the treatment of gout.
Beneficial effect: Chinese medicine extract of the present invention has the curative effect of very good treatment gout.When Radix Angelicae Sinensis water extract is 4 ~ 10 parts, pseudo-ginseng water extract is 7 ~ 13 parts, Rhizoma Corydalis ethanol extraction is 5 ~ 10 parts, Radix Peucedani zymolyte is 4 ~ 9 parts, Radix Et Caulis Acanthopanacis Senticosi zymolyte is 6 ~ 11 parts, Radix Achyranthis Bidentatae water extracting alcohol hypostasis is 6 ~ 15 parts, the Chinese medicine extract treatment gout effect obtained is better, effectively can be used for the treatment of the gout symptom of patient.
Detailed description of the invention
Embodiment 1
(1) first Gansu Angelica is pulverized, being crushed to when being classified as 200 orders, extracting with water after pulverizing, solid-liquid ratio is 1:6, and Extracting temperature is 45 DEG C, and extraction time is 3h, again by extracting solution and residue centrifugalize, after separation, supernatant is carried out evaporation and concentration, after concentrated, obtain Radix Angelicae Sinensis water extract; (2) after complete Radix Notoginseng being plucked, Radix Notoginseng is carried out lyophilization, and lyophilization temperature is-25 DEG C, and drying time is 6h, after drying, Radix Notoginseng is ground, extract with water, solid-liquid ratio is 1:4, and Extracting temperature is 50 DEG C, extraction time is 120min, centrifugalize after extracting, collect supernatant, concentrated supernatant obtains pseudo-ginseng water extract; (3) smashed to pieces by Rhizoma Corydalis, add ethanol and carry out stirring extraction, ethanol extraction temperature is 60 DEG C, and extraction time is 90min, and ethanol weight is 4 times of Rhizoma Corydalis weight, by extracting liquid filtering after extraction, then obtains Rhizoma Corydalis ethanol extraction after filtrate being concentrated; (4) Radix Peucedani is carried out drying, after drying, Radix Peucedani is pulverized, the aqueous solution of cellulase is added in Radix Peucedani, the mass fraction of cellulase is 1wt%, aqueous solution weight is 5 times of Radix Peucedani weight, carries out stirred at ambient temperature enzymolysis, and enzymolysis time is 2h, get upper strata enzymolysis solution after enzymolysis, after enzymolysis solution is concentrated, obtain Radix Peucedani zymolyte; (5) pulverized by Radix Et Caulis Acanthopanacis Senticosi, add cellulase aqueous solution after pulverizing, the concentration of cellulase is 1.5wt%, and solid-liquid ratio is 1:3, carries out stirred at ambient temperature enzymolysis, more concentrated after being collected by upper strata enzymolysis solution, and concentrate is Radix Et Caulis Acanthopanacis Senticosi zymolyte; (6) Radix Achyranthis Bidentatae is shredded, after shredding, Radix Achyranthis Bidentatae water is extracted, solid-liquid ratio is 1:3, Extracting temperature is room temperature, and extraction time is 3h, extracts rear filter-cloth filtering, in filtrate, add ethanol carry out precipitate with ethanol, ethanol addition is 4 times of filtrate weight, collecting precipitation, is Radix Achyranthis Bidentatae water extracting alcohol hypostasis after precipitating drying; (7) getting that Radix Angelicae Sinensis water extract is 6 parts, pseudo-ginseng water extract is 10 parts, Rhizoma Corydalis ethanol extraction is 8 parts, Radix Peucedani zymolyte is 7 parts, Radix Et Caulis Acanthopanacis Senticosi zymolyte is 8 parts, Radix Achyranthis Bidentatae water extracting alcohol hypostasis is 11 parts by weight, will be the Chinese medicine extract for the treatment of gout after above-mentioned each composition mix homogeneously.
Embodiment 2
(1) first Gansu Angelica is pulverized, being crushed to when being classified as 200 orders, extracting with water after pulverizing, solid-liquid ratio is 1:6, and Extracting temperature is 45 DEG C, and extraction time is 3h, again by extracting solution and residue centrifugalize, after separation, supernatant is carried out evaporation and concentration, after concentrated, obtain Radix Angelicae Sinensis water extract; (2) after complete Radix Notoginseng being plucked, Radix Notoginseng is carried out lyophilization, and lyophilization temperature is-25 DEG C, and drying time is 6h, after drying, Radix Notoginseng is ground, extract with water, solid-liquid ratio is 1:4, and Extracting temperature is 50 DEG C, extraction time is 120min, centrifugalize after extracting, collect supernatant, concentrated supernatant obtains pseudo-ginseng water extract; (3) smashed to pieces by Rhizoma Corydalis, add ethanol and carry out stirring extraction, ethanol extraction temperature is 60 DEG C, and extraction time is 90min, and ethanol weight is 4 times of Rhizoma Corydalis weight, by extracting liquid filtering after extraction, then obtains Rhizoma Corydalis ethanol extraction after filtrate being concentrated; (4) Radix Peucedani is carried out drying, after drying, Radix Peucedani is pulverized, the aqueous solution of cellulase is added in Radix Peucedani, the mass fraction of cellulase is 1wt%, aqueous solution weight is 5 times of Radix Peucedani weight, carries out stirred at ambient temperature enzymolysis, and enzymolysis time is 2h, get upper strata enzymolysis solution after enzymolysis, after enzymolysis solution is concentrated, obtain Radix Peucedani zymolyte; (5) getting that Radix Angelicae Sinensis water extract is 4 parts, pseudo-ginseng water extract is 13 parts, Rhizoma Corydalis ethanol extraction is 5 parts, Radix Peucedani zymolyte is 9 parts by weight, will be the Chinese medicine extract for the treatment of gout after above-mentioned each composition mix homogeneously.
Embodiment 3
(1) first Gansu Angelica is pulverized, being crushed to when being classified as 200 orders, extracting with water after pulverizing, solid-liquid ratio is 1:6, and Extracting temperature is 45 DEG C, and extraction time is 3h, again by extracting solution and residue centrifugalize, after separation, supernatant is carried out evaporation and concentration, after concentrated, obtain Radix Angelicae Sinensis water extract; (2) after complete Radix Notoginseng being plucked, Radix Notoginseng is carried out lyophilization, and lyophilization temperature is-25 DEG C, and drying time is 6h, after drying, Radix Notoginseng is ground, extract with water, solid-liquid ratio is 1:4, and Extracting temperature is 50 DEG C, extraction time is 120min, centrifugalize after extracting, collect supernatant, concentrated supernatant obtains pseudo-ginseng water extract; (3) smashed to pieces by Rhizoma Corydalis, add ethanol and carry out stirring extraction, ethanol extraction temperature is 60 DEG C, and extraction time is 90min, and ethanol weight is 4 times of Rhizoma Corydalis weight, by extracting liquid filtering after extraction, then obtains Rhizoma Corydalis ethanol extraction after filtrate being concentrated; (4) Radix Peucedani is carried out drying, after drying, Radix Peucedani is pulverized, the aqueous solution of cellulase is added in Radix Peucedani, the mass fraction of cellulase is 1wt%, aqueous solution weight is 5 times of Radix Peucedani weight, carries out stirred at ambient temperature enzymolysis, and enzymolysis time is 2h, get upper strata enzymolysis solution after enzymolysis, after enzymolysis solution is concentrated, obtain Radix Peucedani zymolyte; (5) getting that Radix Angelicae Sinensis water extract is 10 parts, pseudo-ginseng water extract is 7 parts, Rhizoma Corydalis ethanol extraction is 10 parts, Radix Peucedani zymolyte is 4 parts by weight, will be the Chinese medicine extract for the treatment of gout after above-mentioned each composition mix homogeneously.
Embodiment 4
(1) first Gansu Angelica is pulverized, being crushed to when being classified as 200 orders, extracting with water after pulverizing, solid-liquid ratio is 1:6, and Extracting temperature is 45 DEG C, and extraction time is 3h, again by extracting solution and residue centrifugalize, after separation, supernatant is carried out evaporation and concentration, after concentrated, obtain Radix Angelicae Sinensis water extract; (2) after complete Radix Notoginseng being plucked, Radix Notoginseng is carried out lyophilization, and lyophilization temperature is-25 DEG C, and drying time is 6h, after drying, Radix Notoginseng is ground, extract with water, solid-liquid ratio is 1:4, and Extracting temperature is 50 DEG C, extraction time is 120min, centrifugalize after extracting, collect supernatant, concentrated supernatant obtains pseudo-ginseng water extract; (3) smashed to pieces by Rhizoma Corydalis, add ethanol and carry out stirring extraction, ethanol extraction temperature is 60 DEG C, and extraction time is 90min, and ethanol weight is 4 times of Rhizoma Corydalis weight, by extracting liquid filtering after extraction, then obtains Rhizoma Corydalis ethanol extraction after filtrate being concentrated; (4) Radix Peucedani is carried out drying, after drying, Radix Peucedani is pulverized, the aqueous solution of cellulase is added in Radix Peucedani, the mass fraction of cellulase is 1wt%, aqueous solution weight is 5 times of Radix Peucedani weight, carries out stirred at ambient temperature enzymolysis, and enzymolysis time is 2h, get upper strata enzymolysis solution after enzymolysis, after enzymolysis solution is concentrated, obtain Radix Peucedani zymolyte; (5) pulverized by Radix Et Caulis Acanthopanacis Senticosi, add cellulase aqueous solution after pulverizing, the concentration of cellulase is 1.5wt%, and solid-liquid ratio is 1:3, carries out stirred at ambient temperature enzymolysis, more concentrated after being collected by upper strata enzymolysis solution, and concentrate is Radix Et Caulis Acanthopanacis Senticosi zymolyte; (6) Radix Achyranthis Bidentatae is shredded, after shredding, Radix Achyranthis Bidentatae water is extracted, solid-liquid ratio is 1:3, Extracting temperature is room temperature, and extraction time is 3h, extracts rear filter-cloth filtering, in filtrate, add ethanol carry out precipitate with ethanol, ethanol addition is 4 times of filtrate weight, collecting precipitation, is Radix Achyranthis Bidentatae water extracting alcohol hypostasis after precipitating drying; (7) getting that Radix Angelicae Sinensis water extract is 4 parts, pseudo-ginseng water extract is 13 parts, Rhizoma Corydalis ethanol extraction is 5 parts, Radix Peucedani zymolyte is 9 parts, Radix Et Caulis Acanthopanacis Senticosi zymolyte is 6 parts, Radix Achyranthis Bidentatae water extracting alcohol hypostasis is 15 parts by weight, will be the Chinese medicine extract for the treatment of gout after above-mentioned each composition mix homogeneously.
Embodiment 5
(1) first Gansu Angelica is pulverized, being crushed to when being classified as 200 orders, extracting with water after pulverizing, solid-liquid ratio is 1:6, and Extracting temperature is 45 DEG C, and extraction time is 3h, again by extracting solution and residue centrifugalize, after separation, supernatant is carried out evaporation and concentration, after concentrated, obtain Radix Angelicae Sinensis water extract; (2) after complete Radix Notoginseng being plucked, Radix Notoginseng is carried out lyophilization, and lyophilization temperature is-25 DEG C, and drying time is 6h, after drying, Radix Notoginseng is ground, extract with water, solid-liquid ratio is 1:4, and Extracting temperature is 50 DEG C, extraction time is 120min, centrifugalize after extracting, collect supernatant, concentrated supernatant obtains pseudo-ginseng water extract; (3) smashed to pieces by Rhizoma Corydalis, add ethanol and carry out stirring extraction, ethanol extraction temperature is 60 DEG C, and extraction time is 90min, and ethanol weight is 4 times of Rhizoma Corydalis weight, by extracting liquid filtering after extraction, then obtains Rhizoma Corydalis ethanol extraction after filtrate being concentrated; (4) Radix Peucedani is carried out drying, after drying, Radix Peucedani is pulverized, the aqueous solution of cellulase is added in Radix Peucedani, the mass fraction of cellulase is 1wt%, aqueous solution weight is 5 times of Radix Peucedani weight, carries out stirred at ambient temperature enzymolysis, and enzymolysis time is 2h, get upper strata enzymolysis solution after enzymolysis, after enzymolysis solution is concentrated, obtain Radix Peucedani zymolyte; (5) pulverized by Radix Et Caulis Acanthopanacis Senticosi, add cellulase aqueous solution after pulverizing, the concentration of cellulase is 1.5wt%, and solid-liquid ratio is 1:3, carries out stirred at ambient temperature enzymolysis, more concentrated after being collected by upper strata enzymolysis solution, and concentrate is Radix Et Caulis Acanthopanacis Senticosi zymolyte; (6) Radix Achyranthis Bidentatae is shredded, after shredding, Radix Achyranthis Bidentatae water is extracted, solid-liquid ratio is 1:3, Extracting temperature is room temperature, and extraction time is 3h, extracts rear filter-cloth filtering, in filtrate, add ethanol carry out precipitate with ethanol, ethanol addition is 4 times of filtrate weight, collecting precipitation, is Radix Achyranthis Bidentatae water extracting alcohol hypostasis after precipitating drying; (7) getting that Radix Angelicae Sinensis water extract is 10 parts, pseudo-ginseng water extract is 7 parts, Rhizoma Corydalis ethanol extraction is 10 parts, Radix Peucedani zymolyte is 4 parts, Radix Et Caulis Acanthopanacis Senticosi zymolyte is 11 parts, Radix Achyranthis Bidentatae water extracting alcohol hypostasis is 6 parts by weight, will be the Chinese medicine extract for the treatment of gout after above-mentioned each composition mix homogeneously.
The patient of arthragra symptom is divided into 5 groups, and often organizing number is 10 people, takes the Chinese medicine extract of embodiment 1 to 5 respectively, takes every day once, and each serving with 2g, the cycle of taking is 20 days.Within 20 days, observe the gout treatment situation of patient with gout afterwards.
10 people are after the Chinese medicine extract having taken embodiment 1, and the arthroncus of 8 people, pain, stiff symptom are cured significantly, and the arthroncus of 2 people, pain, stiff symptom are greatly alleviated.
10 people are after the Chinese medicine extract having taken embodiment 2, and the arthroncus of 6 people, pain, stiff symptom are greatly alleviated, and the arthroncus of 4 people, pain, stiff symptom are effectively alleviated.
10 people are after the Chinese medicine extract having taken embodiment 3, and the arthroncus of 7 people, pain, stiff symptom are greatly alleviated, and the arthroncus of 3 people, pain, stiff symptom are effectively alleviated.
10 people are after the Chinese medicine extract having taken embodiment 4, and the arthroncus of 3 people, pain, stiff symptom are cured significantly, and the arthroncus of 7 people, pain, stiff symptom are greatly alleviated.
10 people are after the Chinese medicine extract having taken embodiment 5, and the arthroncus of 5 people, pain, stiff symptom are cured significantly, and the arthroncus of 5 people, pain, stiff symptom are greatly alleviated.
From the effect for the treatment of patient gout, the therapeutic effect of embodiment 1 is best, and the therapeutic effect of embodiment 4 and embodiment 5 is also very good, and the therapeutic effect of embodiment 2 and 3 is better.
Contrast experiment: the Chinese medicine that contrast experiment adopts is Radix Salviae Miltiorrhizae is 5 weight portions, Rhizoma Corydalis is 8 weight portions, Radix Saposhnikoviae is 3 weight portions.Chinese medicine extraction liquid is obtained after decocting.Assay method is with embodiment 1 to 5, and result is: 10 people are taking in contrast experiment after Chinese medicine extraction liquid, and the arthroncus of 3 people, pain, stiff symptom are cured significantly, and the arthroncus of 7 people, pain, stiff symptom are greatly alleviated.
Claims (10)
1. treat a Chinese medicine extract for gout, it is characterized in that the Chinese medicine extract of described treatment gout comprises the extract of following weight parts: Radix Angelicae Sinensis water extract is 4 ~ 10 parts, pseudo-ginseng water extract is 7 ~ 13 parts, Rhizoma Corydalis ethanol extraction is 5 ~ 10 parts, Radix Peucedani zymolyte is 4 ~ 9 parts.
2. a kind of Chinese medicine extract for the treatment of gout according to claim 1, is characterized in that the Chinese medicine extract of described treatment gout comprises the extract of following weight parts: Radix Angelicae Sinensis water extract is 4 ~ 10 parts, pseudo-ginseng water extract is 7 ~ 13 parts, Rhizoma Corydalis ethanol extraction is 5 ~ 10 parts, Radix Peucedani zymolyte is 4 ~ 9 parts, Radix Et Caulis Acanthopanacis Senticosi zymolyte is 6 ~ 11 parts, Radix Achyranthis Bidentatae water extracting alcohol hypostasis is 6 ~ 15 parts.
3. a kind of Chinese medicine extract for the treatment of gout according to claim 2, is characterized in that the Chinese medicine extract of described treatment gout comprises the extract of following weight parts: Radix Angelicae Sinensis water extract is 6 parts, pseudo-ginseng water extract is 10 parts, Rhizoma Corydalis ethanol extraction is 8 parts, Radix Peucedani zymolyte is 7 parts, Radix Et Caulis Acanthopanacis Senticosi zymolyte is 8 parts, Radix Achyranthis Bidentatae water extracting alcohol hypostasis is 11 parts.
4. treat the preparation method of the Chinese medicine extract of gout for one kind, it is characterized in that the preparation method of the Chinese medicine extract of described treatment gout is as follows: (1) extracts with water after Gansu Angelica being pulverized, again extracting solution is separated with residue, after separation, supernatant is concentrated, after concentrated, obtain Radix Angelicae Sinensis water extract; (2) after being plucked by complete Radix Notoginseng, Radix Notoginseng is carried out lyophilization, grinds after drying, extract with water, centrifugalize after extracting, collect supernatant, concentrated supernatant obtains pseudo-ginseng water extract; (3) Rhizoma Corydalis is smashed to pieces, add ethanol and extract, by extracting liquid filtering after extraction, then obtain Rhizoma Corydalis ethanol extraction after filtrate being concentrated; (4) Radix Peucedani is carried out drying, after drying, Radix Peucedani is pulverized, in Radix Peucedani, add water and cellulase, after enzymolysis, get upper strata enzymolysis solution, after enzymolysis solution is concentrated, obtain Radix Peucedani zymolyte; (5) Radix Et Caulis Acanthopanacis Senticosi is pulverized, add cellulase and water after pulverizing, carry out enzymolysis, more concentrated after upper strata enzymolysis solution is collected, obtain Radix Et Caulis Acanthopanacis Senticosi zymolyte; (6) shredded by Radix Achyranthis Bidentatae, shred rear water and extract, filter after extracting, carrying out precipitate with ethanol, collecting precipitation by adding ethanol in filtrate, is Radix Achyranthis Bidentatae water extracting alcohol hypostasis; (7) getting that Radix Angelicae Sinensis water extract is 6 parts, pseudo-ginseng water extract is 10 parts, Rhizoma Corydalis ethanol extraction is 8 parts, Radix Peucedani zymolyte is 7 parts, Radix Et Caulis Acanthopanacis Senticosi zymolyte is 8 parts, Radix Achyranthis Bidentatae water extracting alcohol hypostasis is 11 parts by weight, will be the Chinese medicine extract for the treatment of gout after above-mentioned each composition mix homogeneously.
5. a kind of preparation method for the treatment of the Chinese medicine extract of gout according to claim 4, it is characterized in that in preparation method, Radix Angelicae Sinensis water extract preparation process is as follows: Gansu Angelica first pulverized, be crushed to when being classified as 200 orders, extract with water after pulverizing, solid-liquid ratio is 1:6, and Extracting temperature is 45 DEG C, extraction time is 3h, again by extracting solution and residue centrifugalize, after separation, supernatant is carried out evaporation and concentration, after concentrated, obtain Radix Angelicae Sinensis water extract.
6. a kind of preparation method for the treatment of the Chinese medicine extract of gout according to claim 4, is characterized in that in preparation method, pseudo-ginseng water extract preparation process is as follows: after being plucked by complete Radix Notoginseng, Radix Notoginseng is carried out lyophilization, lyophilization temperature is-25 DEG C, drying time is 6h, is ground by Radix Notoginseng after drying, extracts with water, solid-liquid ratio is 1:4, Extracting temperature is 50 DEG C, and extraction time is 120min, centrifugalize after extracting, collect supernatant, concentrated supernatant obtains pseudo-ginseng water extract.
7. a kind of preparation method for the treatment of the Chinese medicine extract of gout according to claim 4, it is characterized in that in preparation method, Rhizoma Corydalis ethanol extraction preparation process is as follows: smashed to pieces by Rhizoma Corydalis, add ethanol and carry out stirring extraction, ethanol extraction temperature is 60 DEG C, extraction time is 90min, ethanol weight is 4 times of Rhizoma Corydalis weight, by extracting liquid filtering after extraction, then obtains Rhizoma Corydalis ethanol extraction after filtrate being concentrated.
8. a kind of preparation method for the treatment of the Chinese medicine extract of gout according to claim 4, it is characterized in that in preparation method, Radix Peucedani zymolyte preparation process is as follows: Radix Peucedani is carried out drying, after drying, Radix Peucedani is pulverized, in Radix Peucedani, add the aqueous solution of cellulase, the mass fraction of cellulase is 1wt%, and aqueous solution weight is 5 times of Radix Peucedani weight, carry out stirred at ambient temperature enzymolysis, enzymolysis time is 2h, gets upper strata enzymolysis solution after enzymolysis, obtains Radix Peucedani zymolyte after enzymolysis solution is concentrated.
9. a kind of preparation method for the treatment of the Chinese medicine extract of gout according to claim 4, it is characterized in that the preparation process of Radix Et Caulis Acanthopanacis Senticosi zymolyte in preparation method is as follows: pulverized by Radix Et Caulis Acanthopanacis Senticosi, cellulase aqueous solution is added after pulverizing, the concentration of cellulase is 1.5wt%, solid-liquid ratio is 1:3, carry out stirred at ambient temperature enzymolysis, more concentrated after upper strata enzymolysis solution is collected, concentrate is Radix Et Caulis Acanthopanacis Senticosi zymolyte.
10. a kind of preparation method for the treatment of the Chinese medicine extract of gout according to claim 4, it is characterized in that the preparation process of Radix Achyranthis Bidentatae water extracting alcohol hypostasis in preparation method is as follows: shredded by Radix Achyranthis Bidentatae, extracted by Radix Achyranthis Bidentatae water after shredding, solid-liquid ratio is 1:3, and Extracting temperature is room temperature, extraction time is 3h, extract rear filter-cloth filtering, add ethanol and carry out precipitate with ethanol in filtrate, ethanol addition is 4 times of filtrate weight, collecting precipitation will be Radix Achyranthis Bidentatae water extracting alcohol hypostasis after precipitating drying.
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