Disclosure of Invention
In order to solve the technical problems, the invention provides a traditional Chinese medicine composition for treating bovine tuberculosis, which comprises, by weight, 60-80 parts of bletilla striata, 20-30 parts of astragalus membranaceus, 20-30 parts of thunberg fritillary bulb, 8-13 parts of motherwort, 12-16 parts of pseudo-ginseng, 7-10 parts of radix rehmanniae and 4-8 parts of fritillary bulb.
More preferably, the traditional Chinese medicine composition comprises 68 parts by weight of bletilla striata, 24 parts by weight of astragalus membranaceus, 27 parts by weight of thunberg fritillary bulb, 10 parts by weight of motherwort, 13 parts by weight of pseudo-ginseng, 7 parts by weight of radix rehmanniae and 5 parts by weight of fritillary bulb.
Cleaning and drying the bletilla striata, crushing the bletilla striata into 80-120 meshes, putting the bletilla striata into an extractor for extraction for 2 times, wherein the extraction temperature is 34.5-36.8 ℃, and the extraction time is 30-40 min, so as to obtain an extracting solution;
the thunberg fritillary bulb is crushed to 50-100 meshes, added into 5-8 times of water for decoction, the decoction time is 30-60 min, and the mixture is filtered.
The traditional Chinese medicine composition also comprises medically acceptable auxiliary materials.
The invention also aims to provide a preparation method of the feed, which comprises the following steps:
(1) weighing bletilla striata, astragalus membranaceus, thunberg fritillary bulb and motherwort according to a formula, grinding and uniformly mixing, adding 85-90% ethanol with the concentration being 4-6 times of the mass of the bletilla striata, astragalus membranaceus, thunberg fritillary bulb and motherwort, controlling the temperature to be 35-40 ℃, refluxing for 2-3 hours, putting filtrate into thick paste with the vacuum degree of 0.05-0.08 Mpa, concentrating under reduced pressure until the relative density is 1.12-1.15 at 62-66 ℃, and then performing spray drying to obtain powder;
(2) weighing pseudo-ginseng, radix rehmanniae and fritillaria according to a formula, crushing, uniformly mixing, adding 85-90% ethanol with the concentration being 6-8 times of the mass of the pseudo-ginseng, the radix rehmanniae and the fritillaria, controlling the temperature to be 40-45 ℃, refluxing for 1-2 h, putting filtrate into thick paste with the vacuum degree of 0.04-0.06 Mpa, concentrating under reduced pressure to 55-60 ℃ and the relative density of 1.07-1.11, and then spray-drying to obtain powder;
(3) and (3) uniformly mixing the powder obtained in the step (1) and the powder obtained in the step (2) by adopting ultrasound.
The ultrasonic treatment frequency is 5-10 KHz, and the treatment time is 8-15 s.
The invention has the beneficial effects that:
in the scheme, after the bletilla striata is treated, effective substances in the bletilla striata are extracted, and then the bletilla striata is uniformly mixed with other traditional Chinese medicines in the formula in a liquid mode, so that the medicine property of the mixed medicines is enhanced; according to the scheme, after the thunberg fritillary bulb is treated, the effective ingredients in the thunberg fritillary bulb are leached out, and then the effective ingredients are uniformly mixed with other traditional Chinese medicines in the formula in a liquid mode, so that the medicine property of the mixed medicines is enhanced;
the traditional Chinese medicine composition is prepared from bletilla striata, astragalus membranaceus, thunberg fritillary bulb, motherwort herb, pseudo-ginseng, radix rehmanniae and fritillaria bulb as raw materials according to a reasonable proportion, can effectively treat bovine tuberculosis and improve the immunologic function of cattle, and is short in treatment period, remarkable in effect, low in toxicity and safe to eat.
Detailed Description
The technical solution of the present invention is further limited by the following specific embodiments, but the scope of the claims is not limited to the description.
Example 1
The formula is as follows: 60g of bletilla striata, 20g of astragalus membranaceus, 20g of thunberg fritillary bulb, 8g of motherwort, 12g of pseudo-ginseng, 7g of radix rehmanniae and 4g of fritillaria;
the preparation method comprises the following steps: (1) weighing bletilla striata, astragalus membranaceus, thunberg fritillary bulb and motherwort according to a formula, grinding and uniformly mixing, adding 85-90% ethanol with the concentration being 4-6 times of the mass of the bletilla striata, astragalus membranaceus, thunberg fritillary bulb and motherwort, controlling the temperature to be 35-40 ℃, refluxing for 2-3 hours, putting filtrate into thick paste with the vacuum degree of 0.05-0.08 Mpa, concentrating under reduced pressure until the relative density is 1.12-1.15 at 62-66 ℃, and then performing spray drying to obtain powder;
(2) weighing pseudo-ginseng, radix rehmanniae and fritillaria according to a formula, crushing, uniformly mixing, adding 85-90% ethanol with the concentration being 6-8 times of the mass of the pseudo-ginseng, the radix rehmanniae and the fritillaria, controlling the temperature to be 40-45 ℃, refluxing for 1-2 h, putting filtrate into thick paste with the vacuum degree of 0.04-0.06 Mpa, concentrating under reduced pressure to 55-60 ℃ and the relative density of 1.07-1.11, and then spray-drying to obtain powder;
(3) and (3) uniformly mixing the powder obtained in the step (1) and the powder obtained in the step (2) by adopting ultrasound.
The ultrasonic treatment frequency is 5KHz, and the treatment time is 8 s.
Example 2
The formula is as follows: 80g of bletilla striata, 30g of astragalus membranaceus, 30g of thunberg fritillary bulb, 13g of motherwort, 16g of pseudo-ginseng, 10g of radix rehmanniae and 8g of fritillaria;
the preparation method comprises the following steps: (1) weighing bletilla striata, astragalus membranaceus, thunberg fritillary bulb and motherwort according to a formula, grinding and uniformly mixing, adding 85-90% ethanol with the concentration being 4-6 times of the mass of the bletilla striata, astragalus membranaceus, thunberg fritillary bulb and motherwort, controlling the temperature to be 35-40 ℃, refluxing for 2-3 hours, putting filtrate into thick paste with the vacuum degree of 0.05-0.08 Mpa, concentrating under reduced pressure until the relative density is 1.12-1.15 at 62-66 ℃, and then performing spray drying to obtain powder;
(2) weighing pseudo-ginseng, radix rehmanniae and fritillaria according to a formula, crushing, uniformly mixing, adding 85-90% ethanol with the concentration being 6-8 times of the mass of the pseudo-ginseng, the radix rehmanniae and the fritillaria, controlling the temperature to be 40-45 ℃, refluxing for 1-2 h, putting filtrate into thick paste with the vacuum degree of 0.04-0.06 Mpa, concentrating under reduced pressure to 55-60 ℃ and the relative density of 1.07-1.11, and then spray-drying to obtain powder;
(3) and (3) uniformly mixing the powder obtained in the step (1) and the powder obtained in the step (2) by adopting ultrasound.
The ultrasonic treatment frequency is 10KHz, and the treatment time is 15 s.
Example 3
The formula is as follows: 68g of bletilla striata, 24g of astragalus membranaceus, 27g of thunberg fritillary bulb, 10g of motherwort, 13g of pseudo-ginseng, 7g of radix rehmanniae and 5g of fritillaria;
the preparation method comprises the following steps: (1) weighing bletilla striata, astragalus membranaceus, thunberg fritillary bulb and motherwort according to a formula, grinding and uniformly mixing, adding 85-90% ethanol with the concentration being 4-6 times of the mass of the bletilla striata, astragalus membranaceus, thunberg fritillary bulb and motherwort, controlling the temperature to be 35-40 ℃, refluxing for 2-3 hours, putting filtrate into thick paste with the vacuum degree of 0.05-0.08 Mpa, concentrating under reduced pressure until the relative density is 1.12-1.15 at 62-66 ℃, and then performing spray drying to obtain powder;
(2) weighing pseudo-ginseng, radix rehmanniae and fritillaria according to a formula, crushing, uniformly mixing, adding 85-90% ethanol with the concentration being 6-8 times of the mass of the pseudo-ginseng, the radix rehmanniae and the fritillaria, controlling the temperature to be 40-45 ℃, refluxing for 1-2 h, putting filtrate into thick paste with the vacuum degree of 0.04-0.06 Mpa, concentrating under reduced pressure to 55-60 ℃ and the relative density of 1.07-1.11, and then spray-drying to obtain powder;
(3) and (3) uniformly mixing the powder obtained in the step (1) and the powder obtained in the step (2) by adopting ultrasound.
The ultrasonic treatment frequency is 8KHz, and the treatment time is 12 s.
Cleaning rhizoma bletilla, drying, pulverizing to 100 mesh, extracting in an extractor for 2 times at 34.5 deg.C for 30min to obtain extractive solution;
pulverizing Bulbus Fritillariae Thunbergii to 50 mesh, adding 5 times of water, decocting for 30min, and filtering.
Test example 4
The formula is as follows: 70g of bletilla striata, 20g of astragalus membranaceus, 20g of thunberg fritillary bulb, 13g of motherwort, 12-16 g of pseudo-ginseng, 7g of radix rehmanniae and 8g of fritillaria;
the preparation method comprises the following steps: (1) weighing bletilla striata, astragalus membranaceus, thunberg fritillary bulb and motherwort according to a formula, grinding and uniformly mixing, adding 85-90% ethanol with the concentration being 4-6 times of the mass of the bletilla striata, astragalus membranaceus, thunberg fritillary bulb and motherwort, controlling the temperature to be 35-40 ℃, refluxing for 2-3 hours, putting filtrate into thick paste with the vacuum degree of 0.05-0.08 Mpa, concentrating under reduced pressure until the relative density is 1.12-1.15 at 62-66 ℃, and then performing spray drying to obtain powder;
(2) weighing pseudo-ginseng, radix rehmanniae and fritillaria according to a formula, crushing, uniformly mixing, adding 85-90% ethanol with the concentration being 6-8 times of the mass of the pseudo-ginseng, the radix rehmanniae and the fritillaria, controlling the temperature to be 40-45 ℃, refluxing for 1-2 h, putting filtrate into thick paste with the vacuum degree of 0.04-0.06 Mpa, concentrating under reduced pressure to 55-60 ℃ and the relative density of 1.07-1.11, and then spray-drying to obtain powder;
(3) and (3) uniformly mixing the powder obtained in the step (1) and the powder obtained in the step (2) by adopting ultrasound.
The ultrasonic treatment frequency is 7KHz, and the treatment time is 10 s.
Cleaning and drying rhizoma bletilla, pulverizing to 120 mesh, extracting in an extractor for 2 times at 36.8 deg.C for 40min to obtain extractive solution;
pulverizing Bulbus Fritillariae Thunbergii to 100 mesh, adding 8 times of water, decocting for 60min, and filtering.
Example 5
The formula is as follows: 65g of bletilla striata, 28g of astragalus membranaceus, 22g of thunberg fritillary bulb, 11g of motherwort, 15g of pseudo-ginseng, 9g of radix rehmanniae and 5g of fritillaria;
the preparation method comprises the following steps: (1) weighing bletilla striata, astragalus membranaceus, thunberg fritillary bulb and motherwort according to a formula, grinding and uniformly mixing, adding 85-90% ethanol with the concentration being 4-6 times of the mass of the bletilla striata, astragalus membranaceus, thunberg fritillary bulb and motherwort, controlling the temperature to be 35-40 ℃, refluxing for 2-3 hours, putting filtrate into thick paste with the vacuum degree of 0.05-0.08 Mpa, concentrating under reduced pressure until the relative density is 1.12-1.15 at 62-66 ℃, and then performing spray drying to obtain powder;
(2) weighing pseudo-ginseng, radix rehmanniae and fritillaria according to a formula, crushing, uniformly mixing, adding 85-90% ethanol with the concentration being 6-8 times of the mass of the pseudo-ginseng, the radix rehmanniae and the fritillaria, controlling the temperature to be 40-45 ℃, refluxing for 1-2 h, putting filtrate into thick paste with the vacuum degree of 0.04-0.06 Mpa, concentrating under reduced pressure to 55-60 ℃ and the relative density of 1.07-1.11, and then spray-drying to obtain powder;
(3) and (3) uniformly mixing the powder obtained in the step (1) and the powder obtained in the step (2) by adopting ultrasound.
The ultrasonic treatment frequency is 9KHz, and the treatment time is 14 s.
Cleaning and drying the bletilla striata, crushing to 10 meshes, putting into an extractor for extraction for 2 times, wherein the extraction temperature is 35 ℃, and the extraction time is 35min, so as to obtain an extracting solution;
test example 1
The medicine prepared by the scheme of the invention is taken 3 times a day, the dosage is 0.8g/kg, and isoniazid is taken as a control group twice a day by an intravenous injection mode, 1g each time;
the clinical test totally 120 cattle suffered from the disease, the clinical test totally divides into 6 groups, each group randomly has 20 cattle, the test period is 60d, and the test result is as follows:
according to test data, the medicine prepared by the scheme of the invention has obvious effect on treating bovine tuberculosis, wherein the cure rate of the example group is higher than that of the control group, the cure time of the first sick cattle is obviously shorter than that of the control group, and the cure rate is higher than that of the control group, wherein the medicine prepared by the scheme of the example 3 has the best effect, and the cure rate reaches 75%.