CN104255514A - Rapid propagation method of regenerated plantlets of lacquer trees - Google Patents
Rapid propagation method of regenerated plantlets of lacquer trees Download PDFInfo
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- CN104255514A CN104255514A CN201410540495.7A CN201410540495A CN104255514A CN 104255514 A CN104255514 A CN 104255514A CN 201410540495 A CN201410540495 A CN 201410540495A CN 104255514 A CN104255514 A CN 104255514A
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- lacquer tree
- callus
- lacquer
- induction
- root
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Abstract
The invention researches a rapid propagation method of regenerated plantlets of lacquer trees. The method comprises steps as follows: disinfection of lacquer tree leaves, induction of calluses, differentiation of the calluses, root taking induction, seedling exercising, transplantation and the like. According to the method, a plantlet regeneration system of the lacquer trees is established, the growth cycle is short, the cost is low, and a material source and a research basis are provided for the development and the utilization of the lacquer trees.
Description
Technical field
The present invention relates to the quick-breeding method of lacquer tree tissue cultures, belong to field of plant growing technology.
Background technology
Lacquer tree, the large wood paint of another name, little wood paint, mountain is painted, and plants lucerne, blind girl,
toxicodendron vernicifluum, Anacardiaceae, arbor, high 20 meters, happiness light, not resistance toly gives shade; Happiness warm and moist weather and deep fertile and soil that draining is good, acid, neutral and calcium soil all can grow.Not resistance to dry-air blast and severe cold, with on the sunny side, the hillside of wind sheltering, the growth of mountain valley place as well.Not water-fast wet, when having impermeable stratum in the too glutinous heavy particularly soil of soil, easily rotten, even cause death.Lacquer tree is mainly containing laccol, catechol, tannic acid, gallic acid, laccase, lacquer tree Su, Mango tartaric acid, anacardic acid, young fustic, sulphuretin, strawberry tree element, shikimic acid, glucoside etc.In China, most area has plantation, breeds main seeding method, and root is inserted and also can.Hard because of fruit stone crust wax, not easily absorb water germination, needs through dewaxing and vernalization process before sowing.
Summary of the invention
Technical problem to be solved by this invention is to provide the method for quickly breeding of a kind of lacquer tree, and the method establishes the plant regeneration system of lacquer tree, and growth cycle is short, and cost is low, and the development and utilization for lacquer tree provides material source and Research foundation.
Technical problem to be solved by this invention is realized by following scheme:
The leaf cleaning choosing lacquer tree children tender is clean, first immerse in 1% clorox and soak 10min, immerse again in 1% hydrogen peroxide and soak 5min, aseptic water washing 5 times is to noresidue, the induction of callus is carried out, additional saccharose 30g/L, agar 7g/L in the lacquer tree blade access medium White+20ml/L coconut milk of disinfecting, temperature 26 DEG C, intensity of illumination 4W/m
2, every day light application time 5h, PH5.8, the callus derived puts into the differentiation that medium White+KT2-2.5mg/L+NAA0.3-0.5mg/L+ABA4-5mg/L carries out callus, additional saccharose 30g/L, agar 7g/L, temperature 26 DEG C, intensity of illumination 4W/m
2x, every day light application time 12h, PH5.8, when adventitious shoot is about 4cm, cut into single seedling, proceed on MS medium and take root, after one month, seedling grows and is about 5cm root, carry out indoor hardening in laboratory one week, proceed in the matrix of open grave mud+sand mixing and grow, after one month, add up survival rate.
The lacquer tree survival rate adopting the present invention to prepare is high, and the cycle is short, and output is large, pollutes little, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
The leaf cleaning choosing lacquer tree children tender is clean, first immerse in 1% clorox and soak 10min, immerse again in 1% hydrogen peroxide and soak 5min, aseptic water washing 5 times is to noresidue, the induction of callus is carried out, additional saccharose 30g/L, agar 7g/L in the lacquer tree blade access medium White+20ml/L coconut milk of disinfecting, temperature 26 DEG C, intensity of illumination 4W/m
2, every day light application time 5h, PH5.8, the callus derived puts into the differentiation that medium White+KT2.0mg/L+NAA0.3mg/L+ABA4mg/L carries out callus, additional saccharose 30g/L, agar 7g/L, temperature 26 DEG C, intensity of illumination 4W/m
2x, every day light application time 12h, PH5.8, when adventitious shoot is about 4cm, cuts into single seedling, proceed on MS medium and take root, after one month, seedling grows and is about 5cm root, carries out indoor hardening one week in laboratory, proceeds in the matrix of open grave mud+sand mixing and grows, survival rate is added up, survival rate 90% after one month.
Embodiment 2
The leaf cleaning choosing lacquer tree children tender is clean, first immerse in 1% clorox and soak 10min, immerse again in 1% hydrogen peroxide and soak 5min, aseptic water washing 5 times is to noresidue, the induction of callus is carried out, additional saccharose 30g/L, agar 7g/L in the lacquer tree blade access medium White+20ml/L coconut milk of disinfecting, temperature 26 DEG C, intensity of illumination 4W/m
2, every day light application time 5h, PH5.8, the callus derived puts into the differentiation that medium White+KT2.5mg/L+NAA0.4mg/L+ABA5mg/L carries out callus, additional saccharose 30g/L, agar 7g/L, temperature 26 DEG C, intensity of illumination 4W/m
2x, every day light application time 12h, PH5.8, when adventitious shoot is about 4cm, cuts into single seedling, proceed on MS medium and take root, after one month, seedling grows and is about 5cm root, carries out indoor hardening one week in laboratory, proceeds in the matrix of open grave mud+sand mixing and grows, survival rate is added up, survival rate 91% after one month.
Embodiment 3
The leaf cleaning choosing lacquer tree children tender is clean, first immerse in 1% clorox and soak 10min, immerse again in 1% hydrogen peroxide and soak 5min, aseptic water washing 5 times is to noresidue, the induction of callus is carried out, additional saccharose 30g/L, agar 7g/L in the lacquer tree blade access medium White+20ml/L coconut milk of disinfecting, temperature 26 DEG C, intensity of illumination 4W/m
2, every day light application time 5h, PH5.8, the callus derived puts into the differentiation that medium White+KT2.5mg/L+NAA0.5mg/L+ABA5mg/L carries out callus, additional saccharose 30g/L, agar 7g/L, temperature 26 DEG C, intensity of illumination 4W/m
2x, every day light application time 12h, PH5.8, when adventitious shoot is about 4cm, cuts into single seedling, proceed on MS medium and take root, after one month, seedling grows and is about 5cm root, carries out indoor hardening one week in laboratory, proceeds in the matrix of open grave mud+sand mixing and grows, survival rate is added up, survival rate 89% after one month.
Claims (3)
1. a method for quickly breeding for lacquer tree regeneration plant, comprise the sterilization of lacquer tree blade, the induction of callus, the differentiation of callus, root induction, acclimatization and transplants, its key step is as follows:
(1) blade that lacquer tree children is tender is got, disinfection;
(2) induction carrying out callus in the lacquer tree blade access medium White+20ml/L coconut milk that step (1) disinfected is got, additional saccharose 30g/L, agar 7g/L, temperature 26 DEG C, intensity of illumination 4W/m
2, every day light application time 5h, pH5.8;
(3) get the callus that step (2) derives and put into the differentiation that medium White+KT2-2.5mg/L+NAA0.3-0.5mg/L+ABA4-5mg/L carries out callus, additional saccharose 30g/L, agar 7g/L, temperature 26 DEG C, intensity of illumination 4W/m
2x, every day light application time 12h, pH5.8;
(4) get step (3) and break up the indefinite bud cultivating out, carry out field acclimatization and transplants.
2. according to the method for quickly breeding of a kind of lacquer tree regeneration plant according to claim 1, it is characterized in that: the sterilization method of lacquer tree blade described in step (1) is that the leaf cleaning choosing lacquer tree children tender is clean, first immerse in 1% clorox and soak 10min, immerse in 1% hydrogen peroxide again and soak 5min, aseptic water washing 5 times is to noresidue.
3. according to the method for quickly breeding of a kind of lacquer tree regeneration plant according to claim 1, it is characterized in that: in step (4), the hardening off method of lacquer tree is for when adventitious shoot is about 4cm, cut into single seedling, proceed on MS medium and take root, after one month, seedling grows and is about 5cm root, carry out indoor hardening in laboratory one week, proceed in the matrix of open grave mud+sand mixing and grow.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410540495.7A CN104255514A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method of regenerated plantlets of lacquer trees |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410540495.7A CN104255514A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method of regenerated plantlets of lacquer trees |
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| CN104255514A true CN104255514A (en) | 2015-01-07 |
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| CN201410540495.7A Pending CN104255514A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method of regenerated plantlets of lacquer trees |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638461A (en) * | 2015-12-30 | 2016-06-08 | 四川禾木本业农林科技有限公司 | Method for preventing browning of callus of toxicodendron succedaneum |
| CN111357627A (en) * | 2020-03-16 | 2020-07-03 | 山西省农业科学院棉花研究所 | Method for preparing salvia miltiorrhiza aseptic seedlings |
-
2014
- 2014-10-14 CN CN201410540495.7A patent/CN104255514A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638461A (en) * | 2015-12-30 | 2016-06-08 | 四川禾木本业农林科技有限公司 | Method for preventing browning of callus of toxicodendron succedaneum |
| CN111357627A (en) * | 2020-03-16 | 2020-07-03 | 山西省农业科学院棉花研究所 | Method for preparing salvia miltiorrhiza aseptic seedlings |
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Application publication date: 20150107 |