CN111357627A - Method for preparing salvia miltiorrhiza aseptic seedlings - Google Patents
Method for preparing salvia miltiorrhiza aseptic seedlings Download PDFInfo
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- CN111357627A CN111357627A CN202010180198.1A CN202010180198A CN111357627A CN 111357627 A CN111357627 A CN 111357627A CN 202010180198 A CN202010180198 A CN 202010180198A CN 111357627 A CN111357627 A CN 111357627A
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- 241000304195 Salvia miltiorrhiza Species 0.000 title claims abstract description 42
- 235000011135 Salvia miltiorrhiza Nutrition 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 23
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000008223 sterile water Substances 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000005708 Sodium hypochlorite Substances 0.000 claims abstract description 12
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims abstract description 12
- 235000017276 Salvia Nutrition 0.000 claims abstract description 8
- 241001072909 Salvia Species 0.000 claims abstract description 3
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 239000006870 ms-medium Substances 0.000 claims description 4
- 238000012256 transgenic experiment Methods 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 13
- 230000009261 transgenic effect Effects 0.000 abstract description 6
- 238000002791 soaking Methods 0.000 abstract description 4
- 241000251468 Actinopterygii Species 0.000 abstract 2
- 230000035784 germination Effects 0.000 description 14
- 240000007164 Salvia officinalis Species 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 230000007226 seed germination Effects 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 208000032382 Ischaemic stroke Diseases 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- LXNHXLLTXMVWPM-UHFFFAOYSA-N Vitamin B6 Natural products CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 1
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 description 1
- 239000011764 pyridoxine hydrochloride Substances 0.000 description 1
- 229960004172 pyridoxine hydrochloride Drugs 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000019158 vitamin B6 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
本发明涉及丹参种子消毒领域，具体是一种制备丹参无菌苗的方法。包括以下步骤：（1）将丹参种子用4wt%次氯酸钠浸泡5分钟，捞出，备用；（2）将步骤（1）得到的丹参种子置于30wt%过氧化氢浸泡5分钟，捞出，备用；（3）将步骤（2）得到的丹参种子用无菌水冲洗3‑5遍，接种于1/2MS培养基上，培养15‑20天即可得到转基因试验所用的无菌苗。与目前常用的采用田间丹参叶片经消毒和组织培养培育再生苗的方法相比，本发明解决了田间叶片培育受气候限制的问题，可以随时进行无菌苗制备，叶片经组织培养再生无菌苗周期长，一般需2个月以上，本发明用种子种植仅需15‑20天。The invention relates to the field of disinfection of Salvia miltiorrhiza seeds, in particular to a method for preparing sterile seedlings of Salvia miltiorrhiza. The method includes the following steps: (1) soaking the Salvia miltiorrhiza seeds with 4wt% sodium hypochlorite for 5 minutes, fish out and set aside; (2) soak the Salvia miltiorrhiza seeds obtained in step (1) in 30wt% hydrogen peroxide for 5 minutes, fish out and set aside for use (3) The Salvia miltiorrhiza seeds obtained in step (2) are rinsed 3-5 times with sterile water, inoculated on 1/2MS medium, and cultivated for 15-20 days to obtain sterile seedlings used in the transgenic test. Compared with the currently commonly used method of using field salvia leaves to be sterilized and tissue culture to cultivate regenerated seedlings, the invention solves the problem that field leaf cultivation is limited by climate, can prepare sterile seedlings at any time, and regenerate sterile seedlings by tissue culture. The cycle is long, generally needs more than 2 months, and the present invention only needs 15-20 days for seed planting.
Description
技术领域technical field
本发明涉及丹参种子消毒领域，具体是一种制备丹参无菌苗的方法。The invention relates to the field of disinfection of Salvia miltiorrhiza seeds, in particular to a method for preparing sterile seedlings of Salvia miltiorrhiza.
背景技术Background technique
丹参是著名的传统中草药之一，广泛应用于预防和治疗高血脂，心脑血管，和急性缺血性中风等疾病，具有抗氧化，抗肿瘤，抗病毒，消炎抗菌，调控血糖血脂等多种药理活性。利用转基因技术创建药效成分含量高、抗逆性好、经济产量高的丹参新种质材料是目前丹参研究和利用的重要技术。Salvia is one of the well-known traditional Chinese herbal medicines, widely used in the prevention and treatment of hyperlipidemia, cardiovascular and cerebrovascular diseases, and acute ischemic stroke. pharmacological activity. The use of transgenic technology to create new germplasm materials of Salvia miltiorrhiza with high content of medicinal components, good stress resistance and high economic yield is an important technology for the research and utilization of Salvia miltiorrhiza.
制备无菌苗是丹参转基因技术的第一步，是前提步骤，决定了丹参转基因的成败。目前常用的丹参无菌苗制备有两种方法：第一种是用大田带叶柄的丹参叶片消毒，经过组织培养培育无菌苗；具体方法是取带叶柄叶片用流水冲洗，转移至超净工作台中用酒精消毒，在次氯酸钠溶液中浸泡,然后用无菌水冲洗去除次氯酸钠溶液，无菌滤纸吸干水后将叶片切成小块，接种于1/2MS培养诱导实验所需要的无菌组培苗。这种方法存在的问题有：田间叶片经组织培养成苗周期长，一般需1个月至2个月；田间叶片取材受环境影响较大，田间丹参收获后无法取叶片；田间培育丹参叶片费时费力；田间取的叶片较大，消毒难度大。目前文献报道多是采用第一种方法。The preparation of sterile seedlings is the first step in the transgenic technology of Salvia miltiorrhiza. At present, there are two methods for preparing sterile Salvia miltiorrhiza seedlings: the first one is to sterilize the salvia leaves with petioles in the field, and cultivate sterile seedlings through tissue culture; Taichung was sterilized with alcohol, soaked in sodium hypochlorite solution, then rinsed with sterile water to remove sodium hypochlorite solution, dried with sterile filter paper, cut the leaves into small pieces, and inoculated into the sterile tissue culture required for the 1/2MS culture induction experiment. Seedling. The problems of this method are: the field leaves are grown into seedlings through tissue culture, which generally takes 1 month to 2 months; It is laborious; the leaves taken in the field are larger, and the disinfection is difficult. The current literature reports mostly use the first method.
第二种方法是用丹参种子消毒后，种于MS培养基上培育无菌苗。具体方法是，选取饱满的丹参种子，用2％-4％的次氯酸钠浸泡10min，然后用无菌水冲洗3-5遍，种于1/2MS培养基上培育成无菌苗。这种方法较第一种方法的优点是不受外界环境限制，任何时候都可以制备。存在的问题是消毒不彻底，容易污染，种子发芽慢，发芽时间较长，发芽时间长达7-10天。The second method is to sterilize the seeds of Salvia miltiorrhiza and plant them on MS medium to cultivate sterile seedlings. The specific method is to select plump Salvia miltiorrhiza seeds, soak them in 2%-4% sodium hypochlorite for 10 minutes, then rinse them with sterile water for 3-5 times, and plant them on 1/2MS medium and cultivate them into sterile seedlings. The advantage of this method over the first method is that it is not restricted by the external environment and can be prepared at any time. The existing problems are incomplete disinfection, easy pollution, slow germination of seeds, long germination time, and germination time of up to 7-10 days.
为了解决这个问题，发明人曾尝试加大次氯酸钠浓度和浸泡时间，但是加大浓度和时间解决了消毒问题，却降低了丹参种子发芽率，发芽时间也变长了。In order to solve this problem, the inventor tried to increase the concentration and soaking time of sodium hypochlorite, but increasing the concentration and time solved the problem of disinfection, but reduced the germination rate of Salvia miltiorrhiza seeds, and the germination time became longer.
发明内容SUMMARY OF THE INVENTION
本发明为了解决丹参种子消毒不彻底对其发芽率的影响，提供了一种制备丹参无菌苗的方法。The invention provides a method for preparing sterile seedlings of Salvia miltiorrhiza in order to solve the influence of incomplete disinfection of Salvia miltiorrhiza seeds on its germination rate.
本发明是通过以下技术方案实现的：一种制备丹参无菌苗的方法，包括以下步骤：The present invention is achieved through the following technical solutions: a method for preparing sterile seedlings of Salvia miltiorrhiza, comprising the following steps:
(1)将丹参种子用4wt％次氯酸钠浸泡5分钟，捞出，备用；(1) the salvia miltiorrhiza seeds were soaked for 5 minutes with 4wt% sodium hypochlorite, fished out, for subsequent use;
(2)将步骤(1)得到的丹参种子置于30wt％过氧化氢浸泡5分钟，捞出，备用；(2) the Salvia miltiorrhiza seeds obtained in step (1) are placed in 30wt% hydrogen peroxide and soaked for 5 minutes, fished out for subsequent use;
(3)将步骤(2)得到的丹参种子用无菌水冲洗3-5遍，接种于1/2MS培养基上，培养15-20天即可得到转基因试验所用的无菌苗。(3) The Salvia miltiorrhiza seeds obtained in step (2) are washed 3-5 times with sterile water, inoculated on 1/2 MS medium, and cultured for 15-20 days to obtain sterile seedlings used in the transgenic test.
本发明为了解决上述丹参种子消毒中，次氯酸钠浓度小，浸泡时间短，消毒不彻底，易污染；次氯酸钠浓度大，浸泡时间长，丹参种子发芽率受抑制，发芽时间长的问题。本发明采用合适浓度的次氯酸钠结合过氧化氢浸泡消毒的方法，利用过氧化氢同时具备杀菌和种子催芽的功效，不仅提高了消毒的彻底性，降低了污染率，还提高了丹参种子的发芽率和发芽势，大大缩短了发芽时间，提高了丹参无菌苗的制备效率。The present invention solves the problems of low concentration of sodium hypochlorite, short soaking time, incomplete disinfection and easy pollution in the above-mentioned disinfection of salvia seeds; The invention adopts the method of soaking and sterilizing sodium hypochlorite of a suitable concentration combined with hydrogen peroxide, and the hydrogen peroxide has the effects of sterilization and seed germination at the same time, which not only improves the thoroughness of disinfection, reduces the pollution rate, but also improves the germination rate of Salvia miltiorrhiza seeds. and germination potential, greatly shortening the germination time, and improving the preparation efficiency of sterile Salvia miltiorrhiza seedlings.
作为本发明技术方案的进一步改进，无菌水每次冲洗的冲洗时间为1-3min。As a further improvement of the technical solution of the present invention, the flushing time of each flushing with sterile water is 1-3min.
与目前常用的采用田间丹参叶片经消毒和组织培养培育再生苗的方法相比，本发明解决了田间叶片培育受气候限制的问题，可以随时进行无菌苗制备，叶片经组织培养再生无菌苗周期长，一般需2个月以上，本发明用种子种植仅需15-20天。Compared with the currently commonly used method of using field salvia leaves to be sterilized and tissue culture to cultivate regenerated seedlings, the invention solves the problem that field leaf cultivation is limited by climate, can prepare sterile seedlings at any time, and regenerate sterile seedlings by tissue culture. The cycle is long, generally more than 2 months, and the seed planting in the present invention only takes 15-20 days.
与部分单位采用的种子消毒种植技术相比，本发明应用了过氧化氢的杀菌和种子催芽功效，将种子污染率从10％-20％，降低至5％以下。丹参种子发芽时间从7-10天缩短至3-7天，将无菌苗培育时间从20-30天缩短至15-20天。Compared with the seed disinfection planting technology adopted by some units, the present invention applies the sterilization and seed germination effects of hydrogen peroxide, and reduces the seed contamination rate from 10% to 20% to less than 5%. The germination time of Salvia miltiorrhiza seeds is shortened from 7-10 days to 3-7 days, and the cultivation time of sterile seedlings is shortened from 20-30 days to 15-20 days.
具体实施方式Detailed ways
为使本发明的目的、技术方案和优点更加清楚，下面将对本发明的技术方案进行详细的描述。显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动的前提下所得到的所有其它实施方式，都属于本发明所保护的范围。In order to make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail below. Obviously, the described embodiments are only some, but not all, embodiments of the present invention. Based on the embodiments of the present invention, all other implementations obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.
本发明下列实施例中所使用的1/2MS培养基配方(mg/L)为：The 1/2MS medium formula (mg/L) used in the following examples of the present invention is:
大量元素：Lots of elements:
NH4NO3 825，KNO3 950，CaCl2·2H2O 220，MgSO4·7H2O 185，KH2PO4 350。NH 4 NO 3 825, KNO 3 950, CaCl 2 2H 2 O 220, MgSO 4 7H 2 O 185, KH 2 PO 4 350.
微量元素：Trace elements:
KI 0.415，H3BO3 3.1，MnSO4·4H2O 11.15，ZnSO4·7H2O 4.3，Na2MnO4·2H2O0.125，CuSO4·5H2O 0.0125，CoCl2·6H2O 0.0125，FeSO4·7H2O(13.9)+Na2-EDTA·2H2O(18.65)。KI 0.415, H 3 BO 3 3.1, MnSO 4 4H 2 O 11.15, ZnSO 4 7H 2 O 4.3, Na 2 MnO 4 2H 2 O 0.125, CuSO 4 5H 2 O 0.0125, CoCl 2 6H 2 O 0.0125, FeSO4.7H2O (13.9) + Na2 - EDTA.2H2O (18.65).
有机成分：Organic ingredients:
肌醇50，烟酸0.25，盐酸吡哆醇(维生素B6)0.25，盐酸硫胺素(维生素B1)0.25。Inositol 50, niacin 0.25, pyridoxine hydrochloride (vitamin B6) 0.25, thiamine hydrochloride (vitamin B1) 0.25.
实施例1Example 1
一种制备丹参无菌苗的方法，包括以下步骤：A method for preparing sterile seedlings of Salvia miltiorrhiza, comprising the following steps:
(1)将丹参种子用4wt％次氯酸钠浸泡5分钟，捞出，备用；(1) the salvia miltiorrhiza seeds were soaked for 5 minutes with 4wt% sodium hypochlorite, fished out, for subsequent use;
(2)将步骤(1)得到的丹参种子置于30wt％过氧化氢浸泡5分钟，捞出，备用；(2) the Salvia miltiorrhiza seeds obtained in step (1) are placed in 30wt% hydrogen peroxide and soaked for 5 minutes, fished out for subsequent use;
(3)将步骤(2)得到的丹参种子用无菌水冲洗3遍，无菌水每次冲洗的冲洗时间为3min，接种于1/2MS培养基上，培养18天即可得到转基因试验所用的无菌苗。(3) The Salvia miltiorrhiza seeds obtained in step (2) are rinsed 3 times with sterile water, and the rinse time of each rinse with sterile water is 3 min, inoculated on 1/2MS medium, and cultivated for 18 days to obtain the seeds used in the transgenic test. sterile seedlings.
实施例2Example 2
一种制备丹参无菌苗的方法，包括以下步骤：A method for preparing sterile seedlings of Salvia miltiorrhiza, comprising the following steps:
(1)将丹参种子用4wt％次氯酸钠浸泡5分钟，捞出，备用；(1) the salvia miltiorrhiza seeds were soaked for 5 minutes with 4wt% sodium hypochlorite, fished out, for subsequent use;
(2)将步骤(1)得到的丹参种子置于30wt％过氧化氢浸泡5分钟，捞出，备用；(2) the Salvia miltiorrhiza seeds obtained in step (1) are placed in 30wt% hydrogen peroxide and soaked for 5 minutes, fished out for subsequent use;
(3)将步骤(2)得到的丹参种子用无菌水冲洗4遍，无菌水每次冲洗的冲洗时间为2min，接种于1/2MS培养基上，培养17天即可得到转基因试验所用的无菌苗。(3) the salvia seeds obtained in step (2) were rinsed 4 times with sterile water, and the rinse time of each rinse with sterile water was 2min, inoculated on 1/2MS medium, and cultivated for 17 days to obtain the seeds used in the transgenic test. sterile seedlings.
实施例3Example 3
一种制备丹参无菌苗的方法，包括以下步骤：A method for preparing sterile seedlings of Salvia miltiorrhiza, comprising the following steps:
(1)将丹参种子用4wt％次氯酸钠浸泡5分钟，捞出，备用；(1) the salvia miltiorrhiza seeds were soaked for 5 minutes with 4wt% sodium hypochlorite, fished out, for subsequent use;
(2)将步骤(1)得到的丹参种子置于30wt％过氧化氢浸泡5分钟，捞出，备用；(2) the Salvia miltiorrhiza seeds obtained in step (1) are placed in 30wt% hydrogen peroxide and soaked for 5 minutes, fished out for subsequent use;
(3)将步骤(2)得到的丹参种子用无菌水冲洗5遍，无菌水每次冲洗的冲洗时间为1min，接种于1/2MS培养基上，培养15天即可得到转基因试验所用的无菌苗。(3) the salvia seeds obtained in step (2) were rinsed 5 times with sterile water, and the rinse time of each rinse with sterile water was 1 min, inoculated on 1/2 MS medium, and cultivated for 15 days to obtain the seeds used in the transgenic experiment. sterile seedlings.
发芽试验：选用的丹参品种为HLM，该丹参种子成都电子科技大学生命科学院提供，分别按照实施例1-3的方法进行培育，各获得消毒后的丹参种子100粒，分别接种在相同的1/2MS培养基上(具体成分参见前述)；统计种子发芽数据和污染数据，结果参见下表：Germination test: the selected Salvia miltiorrhiza variety is HLM, and the Salvia miltiorrhiza seeds are provided by the School of Life Sciences, Chengdu University of Electronic Science and Technology, and are cultivated according to the methods of Examples 1-3, respectively, and 100 sterilized Salvia miltiorrhiza seeds are obtained, respectively inoculated in the same 1/2 2MS medium (see above for specific components); statistics of seed germination data and pollution data, the results are shown in the following table:
由上表可以看出：本发明应用了过氧化氢的杀菌和种子催芽功效，将种子污染率从10％-20％，降低至5％以下。丹参种子发芽时间从7-10天缩短至3-7天，将无菌苗培育时间从20-30天缩短至15-20天。It can be seen from the above table that the present invention applies the sterilization and seed germination effects of hydrogen peroxide, and reduces the seed contamination rate from 10% to 20% to below 5%. The germination time of Salvia miltiorrhiza seeds is shortened from 7-10 days to 3-7 days, and the cultivation time of sterile seedlings is shortened from 20-30 days to 15-20 days.
以上所述，仅为本发明的具体实施方式，但本发明的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本发明揭露的技术范围内，可轻易想到变化或替换，都应涵盖在本发明的保护范围之内。因此，本发明的保护范围应以所述权利要求的保护范围为准。The above are only specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention. should be included within the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.
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