CN104222018B - A kind of method of artificial mass rearing Phyloseiulus nersimilis - Google Patents
A kind of method of artificial mass rearing Phyloseiulus nersimilis Download PDFInfo
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- CN104222018B CN104222018B CN201310255870.9A CN201310255870A CN104222018B CN 104222018 B CN104222018 B CN 104222018B CN 201310255870 A CN201310255870 A CN 201310255870A CN 104222018 B CN104222018 B CN 104222018B
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- phyloseiulus nersimilis
- tetranychid
- host plant
- nersimilis
- phyloseiulus
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- 238000000034 method Methods 0.000 title claims abstract description 50
- 230000000384 rearing effect Effects 0.000 title claims abstract description 20
- 238000009395 breeding Methods 0.000 claims abstract description 16
- 230000001488 breeding effect Effects 0.000 claims abstract description 16
- 238000011081 inoculation Methods 0.000 claims description 24
- 239000000203 mixture Substances 0.000 claims description 9
- 239000011159 matrix material Substances 0.000 claims description 5
- 241000238631 Hexapoda Species 0.000 claims description 2
- 241000179203 Phytoseiulus persimilis Species 0.000 claims description 2
- 230000001419 dependent effect Effects 0.000 claims description 2
- 230000012010 growth Effects 0.000 claims description 2
- 238000004064 recycling Methods 0.000 claims description 2
- 238000010008 shearing Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 238000011437 continuous method Methods 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 51
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 244000062645 predators Species 0.000 description 1
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Abstract
A kind of method that the present invention relates to artificial mass rearing Phyloseiulus nersimilis, including the method for breeding of tetranychid, the method for breeding first of Phyloseiulus nersimilis and continuous method for breeding.Present invention eliminates the multistep operation in existing Phyloseiulus nersimilis method for breeding; provide the new method of a kind of continuous raising Phyloseiulus nersimilis; can not only reduce the feeding cost of more than 15% further, the volume of production of unit are Phyloseiulus nersimilis can be risen to 30000/square metre by 10000/square metre, and without inoculating the method that also can realize its scale mass rearing when proposing to raise continuously Phyloseiulus nersimilis first.
Description
Technical field
A kind of method that the present invention relates to artificial mass rearing Phyloseiulus nersimilis (Phytoseiuluspersimilis), belongs to agricultural pests field of biological control.
Background technology
Phyloseiulus nersimilis is the special feeding habits Predator of tetranychid, is also both at home and abroad for preventing and treating the Important Natural Enemy of greenhouse tetranychid.
At present; the method for breeding of Phyloseiulus nersimilis can only utilize tetranychid mass rearing; although existing Phyloseiulus nersimilis method for breeding is capable of the scale mass rearing of Phyloseiulus nersimilis; but still there is the defect that cost height, unit are Phyloseiulus nersimilis production scale are relatively low, the volume of production how to reduce feeding cost, increasing unit are is urgently to be resolved hurrily.
Summary of the invention
It is an object of the invention to provide a kind of artificial mass rearing method of volume of production that can reduce Phyloseiulus nersimilis feeding cost, increase unit are Phyloseiulus nersimilis further.Comparing Phyloseiulus nersimilis method for breeding before this, the present invention can reduce the feeding cost of at least 15%；Unit are volume of production improves two times: the volume of production of Phyloseiulus nersimilis is by 10000/m230000/m can be risen to2。
The present invention is that the host plant first with tetranychid raises tetranychid, the method that the tetranychid raised of recycling raises Phyloseiulus nersimilis, but committed step has the very big difference, concrete key operation step to be:
1) raising of tetranychid: launch in the first 0.5 day-15 days to true leaf host plant, blade or plants stems with tetranychid kind source is inoculated on new host plant together with the compositions of blade, tetranychid in inoculation is each demodicid mite state of tetranychid, described blade is not cut into small pieces or is seldom cut into small pieces, wherein new host plant width of blade is at least at more than 1cm, if host plant is Kidney bean, preferred host plant leaf blade mean breadth reaches inoculation during more than 6cm, the tetranychid amount that average every strain host plant is accessed is more than 5, preferably more than 30, the blade for new host plant inoculation or the compositions of stem and blade is removed in 10 days after inoculation, wherein the terminal bud of plant being processed is do not cut or cut less, preferably only cut 1-2 time.
2) raising of Phyloseiulus nersimilis: the whole receptacle raising Phyloseiulus nersimilis is to seal, but can breathe freely, in the process raising Phyloseiulus nersimilis, it is not necessary to dependent insect cage.
The method for breeding first of Phyloseiulus nersimilis: in the process raising Phyloseiulus nersimilis, the inoculation carrier of Phyloseiulus nersimilis is not essential, if having, inoculation carrier can not be singly blade, if selecting blade as carrier, preferably the compositions of plants stems and blade is as the inoculation carrier of Phyloseiulus nersimilis；The each demodicid mite state of Phyloseiulus nersimilis is accessed, it is preferable that every strain tetranychid amount accesses Phyloseiulus nersimilis when reaching more than 500 when every strain tetranychid amount reaches more than 100；The access ratio of tetranychid and Phyloseiulus nersimilis is more than 5: 1, it is preferable that more than 50: 1, less than 1000: 1；Raise between the host plant planting container of Phyloseiulus nersimilis without or less with boom, it is preferable that without boom；Terminal bud process to plant is not cut or cut less, it is preferable that do not cut.
nullNext batch and the method later raising Phyloseiulus nersimilis continuously，Method for breeding can be identical with the method for breeding first of above-mentioned Phyloseiulus nersimilis，But preferably the following stated method for breeding，Concrete operations are: after last batch Phyloseiulus nersimilis is gathered，New is a collection of with tetranychid、The host plant of Phyloseiulus nersimilis to be seeded is not to continue with the method mass rearing Phyloseiulus nersimilis adopting inoculation Phyloseiulus nersimilis to host plant，But after last batch Phyloseiulus nersimilis is gathered within 10 days、Preferably in 1 day，Host plant to be seeded is moved in the receptacle of the complete Phyloseiulus nersimilis of freshly harvested，It is placed on raising frame、Raise platform、Ground、The remaining host plant plantation utensil of the complete Phyloseiulus nersimilis of freshly harvested、Planting matrix or other can see that each of Phyloseiulus nersimilis is local，The host plant plantation utensil that last batch stays is removed after 0.1 day-10 days，Then again put again or shift the host plant stayed，Determine whether directly to gather according to the amount of Phyloseiulus nersimilis on host plant、Or mend the host plant filling out part with tetranychid again、Or continue to raise、Or benefit continues to raise after putting part Phyloseiulus nersimilis again，But preferably no longer mend and put Phyloseiulus nersimilis.
Wherein, the host plant of described tetranychid refers to the plant that can raise tetranychid；The compositions of described plants stems and blade refers to singly does not have blade to also have the side shoot on plants stems or stem, branch, palpus branch in the carrier that tetranychid or Phyloseiulus nersimilis inoculate；Described blade be seldom cut into small pieces refer to blade all or part of be complete, and incomplete part is when shearing with the plant of tetranychid, it is possible to incidentally miscut or accidentally injure the blade fritter that blade is formed；It is do not cut or cut less to refer to and can not cut terminal bud completely or cut terminal bud several times less that described terminal bud processes, rather than always once find that terminal bud is just wiped out in time, it is necessary to member-retaining portion terminal bud or all terminal bud continued growths；Inoculation not essential the referring to of carrier of described Phyloseiulus nersimilis can inoculate the pure Phyloseiulus nersimilis without carrier, it is also possible to inoculates the Phyloseiulus nersimilis with carrier；Described single can be that blade refers to that the inoculation carrier of Phyloseiulus nersimilis can be the carrier of any carrying Phyloseiulus nersimilis；Described plantation utensil refers to for planting the basin of host plant, dish, container etc., and being filled with planting matrix in these utensils, host plant is grown in these planting matrixs.
The present invention is compared with prior art, except access tetranychid, the mode of Phyloseiulus nersimilis, time, amount, ratio etc. different except, differ primarily in that in the process raising tetranychid and Phyloseiulus nersimilis of the present invention, the terminal bud of plant being processed is do not cut or cut less, this guarantees plant can grow just more branch and leaf, raise more tetranychid and Phyloseiulus nersimilis；In the process raising Phyloseiulus nersimilis without or less with boom, this just improves space availability ratio；Present invention firstly provides the method raising Phyloseiulus nersimilis continuously, such that it is able to the method avoiding the mode having been used up inoculation to raise Phyloseiulus nersimilis, reduce again feeding cost further.Present invention eliminates the multistep operation in existing Phyloseiulus nersimilis method for breeding; can not only reduce the feeding cost of more than 15% further, the volume of production of unit are Phyloseiulus nersimilis can be risen to 30000/square metre by 10000/square metre, and without inoculating the method that can realize its scale mass rearing when proposing to raise continuously Phyloseiulus nersimilis first.
Detailed description of the invention
Following methods is only that the embodiment to claim and summary of the invention is illustrated, and does not represent the restriction to present claims and summary of the invention.
The method for breeding of embodiment 1 tetranychid
nullTemperature 20 DEG C-40 DEG C、The raising indoor feeding tetranychid of humidity 75%-90%，First plant host plant，Launch in the first 0.5 day-15 days to true leaf host plant，Blade or plants stems with tetranychid kind source is inoculated on new host plant together with the compositions of blade，Tetranychid in inoculation is each movable mite state of tetranychid，Described blade is not cut into small pieces or is seldom cut into small pieces，Wherein new host plant width of blade is at least at more than 1cm，If host plant is Kidney bean, preferred host plant leaf blade mean breadth reaches inoculation during more than 6cm，The tetranychid amount that average every strain host plant is accessed is more than 5，Preferably more than 30，The blade for new host plant inoculation or the compositions of stem and blade is removed in 0.1 day-10 days after inoculation，Wherein the terminal bud of plant being processed is only cut 1-2 time.
The method for breeding first of embodiment 2 Phyloseiulus nersimilis
At the raising indoor feeding Phyloseiulus nersimilis of temperature 20 DEG C-40 DEG C, humidity 75%-90%, access Phyloseiulus nersimilis when every strain tetranychid amount reaches more than 100, it is preferable that every strain tetranychid amount accesses Phyloseiulus nersimilis when reaching more than 500；The access ratio of tetranychid and Phyloseiulus nersimilis is more than 5: 1, it is preferable that more than 50: 1, less than 1000: 1；Raise between the host plant planting container of Phyloseiulus nersimilis without boom；Terminal bud process to plant is not cut.
The continuous method for breeding of embodiment 3 Phyloseiulus nersimilis
nullAfter last batch Phyloseiulus nersimilis is gathered，New is a collection of with tetranychid、The host plant of Phyloseiulus nersimilis to be seeded is not to continue with the method mass rearing Phyloseiulus nersimilis adopting inoculation Phyloseiulus nersimilis to host plant，But after last batch Phyloseiulus nersimilis is gathered within 10 days、Preferably in 1 day，Host plant to be seeded is moved in the receptacle of the complete Phyloseiulus nersimilis of freshly harvested，It is placed on raising frame、Raise platform、Ground、The remaining host plant plantation utensil of the complete Phyloseiulus nersimilis of freshly harvested、Planting matrix or other can see that each of Phyloseiulus nersimilis is local，The host plant plantation utensil that last batch stays is removed after 0.1 day-10 days，Then again put again or shift the host plant stayed，Determine whether directly to gather according to the amount of Phyloseiulus nersimilis on host plant、Or mend the host plant filling out part with tetranychid again、Or continue to raise、Or benefit continues to raise after putting part Phyloseiulus nersimilis again，But preferably no longer mend and put Phyloseiulus nersimilis.
Claims (8)
1. the method for an artificial mass rearing Phyloseiulus nersimilis, it is characterized in that raising tetranychid first with the host plant of tetranychid, the tetranychid that recycling is raised raises the method for Phyloseiulus nersimilis (Phytoseiuluspersimilis), and concrete key operation step is:
1) raising of tetranychid: launch in the first 0.5 day-15 days to true leaf host plant, blade or plants stems with tetranychid kind source is inoculated on host plant together with the compositions of blade, tetranychid in inoculation is each demodicid mite state of tetranychid, described blade is not cut into small pieces or is seldom cut into small pieces, wherein host plant width of blade is at more than 1cm, the tetranychid amount that average every strain host plant is accessed is more than 5, the blade for host plant inoculation or the compositions of axis and blade is removed in 10 days after inoculation, wherein the terminal bud of plant being processed is do not cut or cut less；
2) raising of Phyloseiulus nersimilis: the whole receptacle raising Phyloseiulus nersimilis is to seal, but can breathe freely, in the process raising Phyloseiulus nersimilis, it is not necessary to dependent insect cage,
The method for breeding first of Phyloseiulus nersimilis: in the process raising Phyloseiulus nersimilis, the inoculation carrier of Phyloseiulus nersimilis is not essential, if having, inoculation carrier can not be singly blade, the each demodicid mite state of Phyloseiulus nersimilis is accessed when every strain tetranychid amount reaches more than 100, the access ratio of tetranychid and Phyloseiulus nersimilis is more than 5: 1, raise between the host plant planting container of Phyloseiulus nersimilis without or less with boom, it is do not cut or cut less that the terminal bud of plant is processed；
nullNext batch and the method later raising Phyloseiulus nersimilis continuously: after last batch Phyloseiulus nersimilis is gathered，New is a collection of with tetranychid、The host plant of Phyloseiulus nersimilis to be seeded is not to continue with the method mass rearing Phyloseiulus nersimilis adopting inoculation Phyloseiulus nersimilis to host plant，But after last batch Phyloseiulus nersimilis is gathered within 10 days，Host plant to be seeded is moved in the receptacle of the complete Phyloseiulus nersimilis of freshly harvested，It is placed on raising frame、Raise platform、Ground、The remaining host plant plantation utensil of the complete Phyloseiulus nersimilis of freshly harvested、Planting matrix or other can see that each of Phyloseiulus nersimilis is local，The host plant plantation utensil that last batch stays is removed after 0.1 day-10 days，Then again put again or shift the host plant stayed，Determine whether directly to gather according to the amount of Phyloseiulus nersimilis on host plant、Or mend the host plant filling out part with tetranychid again、Or continue to raise、Or benefit continues to raise after putting part Phyloseiulus nersimilis again.
2. the method for a kind of artificial mass rearing Phyloseiulus nersimilis according to claim 1, is characterized in that the host plant of described tetranychid refers to the plant that can raise tetranychid.
3. the method for a kind of artificial mass rearing Phyloseiulus nersimilis according to claim 1, it is characterized in that the compositions of described plants stems and blade refer to singly do not have in the carrier that tetranychid or Phyloseiulus nersimilis inoculate blade also have the side shoot on plants stems or stem, branch, must branch.
4. the method for a kind of artificial mass rearing Phyloseiulus nersimilis according to claim 1, it is characterized in that described blade be seldom cut into small pieces refer to blade all or part of be complete, and incomplete part is when shearing the plant with tetranychid, it is possible to incidentally miscut or accidentally injure the blade fritter that blade is formed.
5. the method for a kind of artificial mass rearing Phyloseiulus nersimilis according to claim 1, it is characterized in that described terminal bud processes is do not cut or cut less to refer to and can not cut terminal bud completely or cut terminal bud several times less, rather than always once find that terminal bud is just wiped out in time, it is necessary to member-retaining portion terminal bud or all terminal bud continued growths.
6. the method for a kind of artificial mass rearing Phyloseiulus nersimilis according to claim 1, it is characterized in that inoculation not essential the referring to of carrier of described Phyloseiulus nersimilis can inoculate the pure Phyloseiulus nersimilis without carrier, it is also possible to inoculate the Phyloseiulus nersimilis with carrier.
7. the method for a kind of artificial mass rearing Phyloseiulus nersimilis according to claim 1, single described in it is characterized in that can be that blade refers to that the inoculation carrier of Phyloseiulus nersimilis can be the carrier of any carrying Phyloseiulus nersimilis.
8. the method for a kind of artificial mass rearing Phyloseiulus nersimilis according to claim 1, is characterized in that described plantation utensil refers to the container for planting host plant, is filled with planting matrix in these utensils, and host plant is grown in these planting matrixs.
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| CN115281158B (en) * | 2022-08-15 | 2023-09-15 | 绵阳市农业科学研究院 | Method for large-scale propagation of phytoseiid chile |
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