CN104222017B - A kind of method of a large amount of production Amblyseius persidolongispinosus - Google Patents
A kind of method of a large amount of production Amblyseius persidolongispinosus Download PDFInfo
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- CN104222017B CN104222017B CN201310255843.1A CN201310255843A CN104222017B CN 104222017 B CN104222017 B CN 104222017B CN 201310255843 A CN201310255843 A CN 201310255843A CN 104222017 B CN104222017 B CN 104222017B
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Abstract
A kind of method that the present invention relates to a large amount of production Amblyseius persidolongispinosus, is characterized in that including the method for breeding of tetranychid, the raising first of Amblyseius persidolongispinosus and continuous method for breeding, the method that separates and collects of Amblyseius persidolongispinosus, the packing method of Amblyseius persidolongispinosus and the process of planting matrix and recycling method five part.Present invention eliminates the multistep operation in existing Amblyseius persidolongispinosus method for breeding; feeding cost can not only be reduced further; and propose the method that also can realize its scale mass rearing without inoculation when raising Amblyseius persidolongispinosus continuously first, and separation, the method collecting and packing Amblyseius persidolongispinosus and saving utilize the way of planting matrix.
Description
Technical field
A kind of method that the present invention relates to a large amount of production Amblyseius persidolongispinosus, belongs to agricultural pests field of biological control.
Background technology
Amblyseius persidolongispinosus is the Important Natural Enemy of preventing and treating tetranychid, it is possible to use tetranychid mass rearing.
Although mentioning the method for breeding of Amblyseius persidolongispinosus in number of patent application 200910158229.7, this method for breeding is capable of
The mass rearing of Amblyseius persidolongispinosus and separation, packaging, but there is also raising operating efficiency is not the highest problem, method for breeding
In mentioned separation, the method for collecting and pack Amblyseius persidolongispinosus be not preferred plan.
How to raise Amblyseius persidolongispinosus and could operate easier, the lower exploration that needs of cost；How to separate, collect and wrap
Dress Amblyseius persidolongispinosus could be better, needs to solve.
Summary of the invention
It is an object of the invention to provide a kind of time saving and energy saving, easy and simple to handle, method for breeding of low cost.
The present invention is that the host plant first with tetranychid raises tetranychid, and the tetranychid that recycling is raised raises the side of Amblyseius persidolongispinosus
Method, concrete key operation step is:
1) raising of tetranychid: in host plant launches the first 0.5 day-15 days to true leaf, by the blade with tetranychid kind source
Or plants stems is inoculated on new host plant together with the composition of blade, the tetranychid in inoculation is each mite state of tetranychid, comprises children
Mite, described blade is not cut into small pieces or is seldom cut into small pieces, and wherein new host plant width of blade is at least at more than 1cm, if posting
Main plant is that Kidney bean then preferred host plant leaf blade mean breadth reaches inoculation during more than 6cm, and average every strain host plant is accessed
Tetranychid amount is more than 5, preferably more than 30, removes for new host plant inoculation in 0.1 day-10 days after inoculation
Blade or stem and the composition of blade, wherein the terminal bud to plant processes is not cut or cut less, the most only cuts 1-2 time.
Wherein, the composition of described plants stems and blade refer to for tetranychid inoculation carrier in the most singly have blade also have plants stems or
Side shoot on stem, branch, palpus branch；Described blade is seldom cut into small pieces and refers to that blade is completely or partially complete, and imperfect
Part be when shearing with the plant of tetranychid, may incidentally miscut or accidentally injure the blade fritter that blade is formed；Described terminal bud
Process is not cut or cut less refer to not cut terminal bud or cut terminal bud rather than always once find that terminal bud is the most several times less
Wipe out, need member-retaining portion terminal bud or all terminal bud continued growths.
2) raising of Amblyseius persidolongispinosus: the whole receptacle raising Amblyseius persidolongispinosus is to seal, but can breathe freely, and is raising
During supporting Amblyseius persidolongispinosus, it is not necessary to dependent insect cage.
The method for breeding first of Amblyseius persidolongispinosus: during raising Amblyseius persidolongispinosus, the inoculation of Amblyseius persidolongispinosus carries
Body is not essential, if having, inoculation carrier can be the most singly blade, if selection blade is as carrier, and preferably plants stems and blade
Composition as the inoculation carrier of Amblyseius persidolongispinosus, described for not being cut into small pieces as the blade of Amblyseius persidolongispinosus carrier
Or be seldom cut into small pieces；Amblyseius persidolongispinosus each mite state, preferably every strain tetranychid is accessed when every strain tetranychid amount reaches more than 100
Amount reaches to access Amblyseius persidolongispinosus when more than 400；The access ratio of tetranychid and Amblyseius persidolongispinosus is more than 5: 1, the most greatly
In 40: 1, less than 1000: 1；Terminal bud process to plant is not cut or cut less, does not cuts.
Next batch and the method raising Amblyseius persidolongispinosus the most continuously, method for breeding can be with the head of above-mentioned Amblyseius persidolongispinosus
Secondary method for breeding is identical, but preferably the following stated method for breeding, concrete operations are: after last batch Amblyseius persidolongispinosus is gathered,
The new a collection of host plant with tetranychid, Amblyseius persidolongispinosus to be seeded is not to continue with using inoculation Amblyseius persidolongispinosus to host
Method mass rearing Amblyseius persidolongispinosus on plant, but after last batch Amblyseius persidolongispinosus is gathered within 10 days, preferably
In 1 day, host plant to be seeded is moved in the receptacle of the complete Amblyseius persidolongispinosus of freshly harvested, be placed on raising frame, raising
Platform, ground, the complete Amblyseius persidolongispinosus of freshly harvested remaining host plant plantation utensil, planting matrix or other can see that plan becomes mildewed
Each of amblyseius as predatory mite is local, removes the host plant plantation utensil that last batch stays, the most again put after 0.1 day-10 days
Or the host plant that transfer stays, determine whether directly to gather or mend again to fill out portion according to the amount of Amblyseius persidolongispinosus on host plant
Point with the host plant of tetranychid or continue to raise or mend again to put to continue after part Amblyseius persidolongispinosus to raise, but the most no longer
Benefit puts Amblyseius persidolongispinosus.
Wherein, the composition of described plants stems and blade refers to the most singly have blade to also have in the carrier of Amblyseius persidolongispinosus inoculation
Side shoot in plants stems or stem, branch, palpus branch；Described blade is seldom cut into small pieces and refers to that blade is completely or partially complete,
And incomplete part is when shearing the plant with Amblyseius persidolongispinosus, may incidentally miscut or accidentally injure the leaf that blade is formed
Sheet fritter；It is not cut or cut less refer to not cut terminal bud or cut terminal bud rather than always several times less that described terminal bud processes
Denier finds that terminal bud is wiped out the most in time, needs member-retaining portion terminal bud or all terminal bud continued growths；The inoculation of described Amblyseius persidolongispinosus
Not essential the referring to of carrier can inoculate the pure Amblyseius persidolongispinosus without carrier, it is also possible to inoculates the plan with carrier and becomes mildewed blunt peaceful
Mite；Described single can be that blade refers to that the inoculation carrier of Amblyseius persidolongispinosus can be the load of any carrying Amblyseius persidolongispinosus
Body；Described plantation utensil refers to, for planting the basin of host plant, dish, container etc., be filled with planting matrix in these utensils,
Host plant is grown in these planting matrixs.
3) method that separates and collects of Amblyseius persidolongispinosus: when the Amblyseius persidolongispinosus number of animals raised reaches the amount of gathering, so that it may plan is become mildewed blunt
Peaceful mite carries out gathering, collecting, and collection method is to cut host plant with scissors, then plants stems, branch is put into together with leaf receipts
In acquisition means, it is possible to individually blade or the branches and leaves of clip host plant are put in collection device, seal collection device the most as early as possible,
Again collection device is placed in suitable space, fixes the collection that i.e. can be used for Amblyseius persidolongispinosus each movable mite state after putting well, receive
Collection operation is every weekly check, collects more than 1 time, preferably checks every day, collects more than 1 time, until collecting pipe is collected less than plan
Becoming mildewed till amblyseius as predatory mite, collecting pipe is that length is within 1m, for collecting the container of Amblyseius persidolongispinosus, it is ensured that collect less than plan
Become mildewed and can clear up the plants stems in collection device, branch or leaf after amblyseius as predatory mite.
Wherein, described collection device refers to the utensil for collecting Amblyseius persidolongispinosus, and collection device can be made up of two parts also may be used
Being made up of three parts, preferably collection device is made up of three parts, if collection device is made up of two parts, including collecting container and receipts
Collector two parts, when collecting Amblyseius persidolongispinosus, directly and coated lycopene, Amblyseius persidolongispinosus is to hold from collecting to collecting pipe
Directly climb in collecting pipe in device；If collection device is made up of three parts, including collecting container, being contained in the collection collected on container
Conduit and collecting pipe three part, when collecting Amblyseius persidolongispinosus, collecting pipe is directly connected with collecting duct, and Amblyseius persidolongispinosus is
Climb to again in collecting pipe in first climbing to collecting duct in collecting container.
Described collection device is placed on suitable space and refers to be designed for the space that Amblyseius persidolongispinosus is collected, it is desirable to this space is being intended
The amblyseius as predatory mite that becomes mildewed has light unglazed when collecting, but changes in collecting pipe, cleaning collection device in fixing collection device, inspection
Light is needed when plants stems, branch or leaf.
Described collection container refers to for loading Amblyseius persidolongispinosus and the part of plants stems, branch or leaf, collects container and has 0.001m3-1m3Volume, collect the manufacture material of container must contain gauze, gauze, cloth, fly net, paper or other can breathe freely and can
Effectively stop the material that mite class is escaped, described gauze, gauze, cloth, fly net, paper or other can breathe freely and can effectively stop mite
But the material that class is escaped can be used alone also that two or more is used in mixed way, collect container and must also have preferably sealing
Property, it is possible to ensure that Amblyseius persidolongispinosus cannot climb out of from other place in addition to climbing in collecting pipe as far as possible, collect container and also have
The opening being easily opened and sealing, opens opening and can load Amblyseius persidolongispinosus and plants stems, branch or leaf, seal opening
Seal and collect container, wherein collect on container for loading Amblyseius persidolongispinosus and plants stems, branch, the opening of leaf become mildewed more than plan
The opening that amblyseius as predatory mite climbs out of in collecting container.
Described collecting duct refers to be contained in collects the passage climbed out of on container for Amblyseius persidolongispinosus, and Amblyseius persidolongispinosus can be from
Collecting duct is contained in that collect on container and climbs out of collection container, then connect together from collecting duct and collecting pipe that
Crawling into collecting pipe, the collecting duct number collected on container is one or more, and the length of collecting duct is within 1m.
4) packing method of Amblyseius persidolongispinosus: after utilizing collection device to collect Amblyseius persidolongispinosus, can directly pack and also may be used
Packing the when of first refrigerating to be needed, packing method is by packaging standard amount, puts into Amblyseius persidolongispinosus with vermiculite or wood again
In the Packaging Bottle of bits or vermiculite and wood chip mixture, the most quickly cover tightly bottle cap, it is desirable to Packaging Bottle body and bottle cap can be the closeest
Sealing, can effectively stop mite class to be escaped, Amblyseius persidolongispinosus can apply or continue refrigeration after having packed, refrigerated storage temperature is 0
DEG C-20 ° of C, preferably 3 DEG C-8 DEG C, described Packaging Bottle bottle cap is with air-vent, and air-vent size, shape are not fixed, but air-vent
Need to be sealed by paper or gauze, gauze, cloth, preferably be sealed by paper.
5) process of planting matrix and re-using: first remove used planting matrix in tetranychid or Amblyseius persidolongispinosus receptacle
Matter, it is possible to the plantation utensil being used in connection with removes receptacle together, after removing or pour out used planting matrix, places 0.1
More than it, it is continuing with after directly sieving or smash with pulverizer after used planting matrix certain mass dryness fraction to be achieved, Qi Zhongruo
Sieving, screen sizes is 0.1cm-10cm, screen cloth planting matrix can be allowed to pass through and can prevent host plant root, stem, leaf or its
Its foreign material passes through, and the root, stem and leaf of host plant can be continuing with after smashing in pulverizer or directly abandon, after sieving or after smashing
The planting matrix stayed can directly utilize or be used in mixed way with new planting matrix or add other and be conducive to what host plant grew to become
Point, but preferably through freezing, high temperature deinsectization sterilizing or process of being exposed to the sun before utilizing these used planting matrixs, to guarantee to plant
Plant Medium Culture and there is no Amblyseius persidolongispinosus, tetranychid or other insects, pathogenic bacteria etc..Wherein, described plantation utensil refers to for planting
Planting the basin of host plant, dish, container etc., be filled with planting matrix in these utensils, host plant is grown in these planting matrixs
In.
The present invention compared with prior art, removes and accesses tetranychid, the mode of Amblyseius persidolongispinosus, time, amount, ratio etc. the most no
With outward, the main distinction also resides in the present invention and is raising tetranychid or be all inoculation during raising Amblyseius persidolongispinosus
Tetranychid and each mite state of Amblyseius persidolongispinosus, it is not necessary to pick into mite, which saves the time, reduce human cost；To leaf
The operating aspect that mite host plant is pruned, the present invention preferably prunes 1-2 time, the terminal bud grown after needing to retain host plant,
So be conducive to raising more tetranychid and Amblyseius persidolongispinosus；The present invention proposes a kind of plan of raising continuously first and becomes mildewed blunt peaceful
The new method of mite, such that it is able to avoid the method that the mode having been used up inoculation raises Amblyseius persidolongispinosus, it is possible to make full use of receipts
The last batch Amblyseius persidolongispinosus that collection is walked stays remaining Amblyseius persidolongispinosus as kind of a source, thus reduces the most again and raises into
This；Amblyseius persidolongispinosus separate and collection in terms of, present invention firstly provides a kind of new collection device, this device have light without
All can realize the collection of Amblyseius persidolongispinosus in the case of light, wherein the collection container in collection device can not use plastics completely
Film, the most completely can without reinforcing bar or reinforcing bar, without support, collect be used on container loading Amblyseius persidolongispinosus and plants stems,
The opening that branch, the opening of leaf climb out of in collecting container more than Amblyseius persidolongispinosus, compares lower the actual collecting effect of collection method
Can be higher, cost is lower, is more suitable for a large amount of collections of Amblyseius persidolongispinosus；In terms of packaging, the present invention uses bottled, it is to avoid
The problem that packed Amblyseius persidolongispinosus there may be extruding；In terms of the re-using of planting matrix, present invention firstly provides weight
The method newly utilizing these planting matrixs, it is possible to more effectively save and utilize resource, thus reduce cost.
Present invention eliminates the multistep operation in existing Amblyseius persidolongispinosus method for breeding, feeding cost can not only be reduced further,
And the method that also can realize its scale mass rearing without inoculation when raising Amblyseius persidolongispinosus continuously, Yi Jifen are proposed first
From, collect and pack the new method of Amblyseius persidolongispinosus and saving utilizes the way of planting matrix.
Detailed description of the invention
Following methods is only the citing of the embodiment to claim and summary of the invention, does not represent present claims and summary of the invention
Restriction.
The method for breeding of embodiment 1 tetranychid
Raising indoor feeding tetranychid between temperature 15 DEG C-45 DEG C, humidity 50%-100%, first plants host plant, host
Plant is launched first in 0.5 day-15 days of true leaf, by with the blade in tetranychid kind source or the composition one of plants stems and blade
Rising and be inoculated on new host plant, the tetranychid in inoculation is each movable mite state of tetranychid, and described blade is not cut into small pieces or seldom cuts
Becoming fritter, wherein new host plant width of blade is at least at more than 1cm, if host plant is Kidney bean, and preferred host plant leaf blade
Mean breadth reaches inoculation during more than 6cm, and the tetranychid amount that average every strain host plant is accessed is more than 5, preferably more than 30,
The blade or stem and the composition of blade inoculated for new host plant is removed in 0.1 day-10 days after inoculation, the most right
It is not cut or cut less that the terminal bud of plant processes, and the most only cuts 1-2 time.
Wherein, the composition of described plants stems and blade refer to for tetranychid inoculation carrier in the most singly have blade also have plants stems or
Side shoot on stem, branch, palpus branch；Described blade is seldom cut into small pieces and refers to that blade is completely or partially complete, and imperfect
Part be when shearing with the plant of tetranychid, may incidentally miscut or accidentally injure the blade fritter that blade is formed；Described terminal bud
Process is not cut or cut less refer to not cut terminal bud or cut terminal bud rather than always once find that terminal bud is the most several times less
Wipe out, need member-retaining portion terminal bud or all terminal bud continued growths.
The method for breeding first of embodiment 2 Amblyseius persidolongispinosus
Raising indoor feeding Amblyseius persidolongispinosus between temperature 15 DEG C-45 DEG C, humidity 50%-100%, raises plan and becomes mildewed blunt peaceful
The whole receptacle of mite is to seal, but can breathe freely, during raising Amblyseius persidolongispinosus, it is not necessary to dependent insect cage.
During raising Amblyseius persidolongispinosus, the inoculation carrier of Amblyseius persidolongispinosus is not essential, if having, inoculation carrier is not
List can be blade, if selection blade is as carrier, and preferably plants stems and composition the connecing as Amblyseius persidolongispinosus of blade
Plant carrier；Access Amblyseius persidolongispinosus when every strain tetranychid amount reaches more than 100, preferably every strain tetranychid amount reach 600 with
Amblyseius persidolongispinosus was accessed time upper；The access ratio of tetranychid and Amblyseius persidolongispinosus is more than 5: 1, preferably greater than 60: 1, is less than
1000∶1；Terminal bud process to plant is not cut or cut less, does not cuts.
Wherein, the composition of described plants stems and blade refers to the most singly have blade to also have in the carrier of Amblyseius persidolongispinosus inoculation
Side shoot in plants stems or stem, branch, palpus branch；It is not cut or cut less to refer to not cut terminal bud or few that described terminal bud processes
Cut terminal bud rather than always once find that terminal bud is wiped out the most in time several times, need member-retaining portion terminal bud or all terminal bud continued growths；
Inoculation not essential the referring to of carrier of described Amblyseius persidolongispinosus can inoculate the pure Amblyseius persidolongispinosus without carrier, it is also possible to connects
Plant the Amblyseius persidolongispinosus with carrier；Described single can be that blade refers to that the inoculation carrier of Amblyseius persidolongispinosus can be to appoint
The carrier of what carrying Amblyseius persidolongispinosus.
The continuous method for breeding of embodiment 3 Amblyseius persidolongispinosus
After last batch Amblyseius persidolongispinosus is gathered, new is a collection of with tetranychid, the host plant of Amblyseius persidolongispinosus to be seeded
It is not to continue with the method mass rearing Amblyseius persidolongispinosus using inoculation Amblyseius persidolongispinosus to host plant, but at last consignment of
Within secondary Amblyseius persidolongispinosus is gathered latter 10 days, in preferably 1 day, host plant to be seeded is moved into the complete plan of freshly harvested and becomes mildewed blunt
Pacify in the receptacle of mite, be placed on raising frame, raise platform, ground, the complete Amblyseius persidolongispinosus of freshly harvested remaining host plant kind
Plant utensil, planting matrix or other can see that each of Amblyseius persidolongispinosus is local, remove last batch after 0.1 day-10 days and stay
Host plant plantation utensil, the most again put or shift the host plant stayed, according on host plant intend become mildewed blunt pacify
The amount of mite determines whether directly to gather or mends and fills out part and with the host plant of tetranychid or continue to raise or mend to put part
Continue after Amblyseius persidolongispinosus to raise, but the most no longer mend and put Amblyseius persidolongispinosus.Wherein, described plantation utensil refers to for planting
Planting the basin of host plant, dish, container etc., be filled with planting matrix in these utensils, host plant is grown in these planting matrixs
In.
The collection method of embodiment 4 Amblyseius persidolongispinosus
When the Amblyseius persidolongispinosus number of animals raised reaches the amount of gathering, so that it may gather Amblyseius persidolongispinosus, collect, collection method is
Cut host plant with scissors, then plants stems, branch are put in collection device together with leaf, it is possible to individually clip host plant
Blade put in collection device, seal collection device the most as early as possible, then collection device be placed in suitable space, fixing put
I.e. can be used for the collection of Amblyseius persidolongispinosus each movable mite state after Hao, collect operation and be every weekly check, collect more than 1 time, preferably
Every day check, collect more than 1 time, until collecting pipe collect less than Amblyseius persidolongispinosus till, collecting pipe be length within 1m,
For collecting the container of Amblyseius persidolongispinosus, it is ensured that collect less than the plants stems can cleared up after Amblyseius persidolongispinosus in collection device,
Branch or leaf.
Wherein, described collection device refers to the utensil for collecting Amblyseius persidolongispinosus, and collection device can be made up of two parts also may be used
Being made up of three parts, preferably collection device is made up of three parts, if collection device is made up of two parts, including collecting container and receipts
Collector two parts, when collecting Amblyseius persidolongispinosus, directly and coated lycopene, Amblyseius persidolongispinosus is to hold from collecting to collecting pipe
Directly climb in collecting pipe in device；If collection device is made up of three parts, including collecting container, being contained in the collection collected on container
Conduit and collecting pipe three part, when collecting Amblyseius persidolongispinosus, collecting pipe is directly connected with collecting duct, and Amblyseius persidolongispinosus is
Climb to again in collecting pipe in first climbing to collecting duct in collecting container；
Described collection device is placed on suitable space and refers to be designed for the space that Amblyseius persidolongispinosus is collected, it is desirable to this space is being intended
The amblyseius as predatory mite that becomes mildewed has light unglazed when collecting, but changes in collecting pipe, cleaning collection device in fixing collection device, inspection
Need light when plants stems, branch or leaf, collecting chamber temperature 15 ° of C-45 ° of C, humidity between 30%-100%；
Described collection container refers to for loading Amblyseius persidolongispinosus and the part of plants stems, branch or leaf, collects container and has 0.001m3-1m3Volume, its surface area more than 10%, preferably 50% be above being made up of gauze, gauze, cloth, fly net or paper, its
Inside can have support also can without support, preferably without support, can have in collection device reinforcing bar, reinforcing bar also can nothing, preferably without；Collect
Container must also have preferable sealing, it is possible to ensure that Amblyseius persidolongispinosus cannot be from other in addition to climbing in collecting pipe as far as possible
Place climbs out of, collect container also have can the opening of free switch, open opening can load Amblyseius persidolongispinosus and plants stems,
Branch or leaf, close upper shed and can seal collection container, wherein collect be used on container loading Amblyseius persidolongispinosus and plants stems, branch,
The opening that the opening of leaf climbs out of in collecting container more than Amblyseius persidolongispinosus；
Described collecting duct refers to be contained in the passage climbed out of for Amblyseius persidolongispinosus collected on container, and Amblyseius persidolongispinosus is permissible
Climb out of from being contained in one, the conduit collected container, then crawl into collecting pipe from the other end connected together with collecting pipe, collect container
On collecting duct number be one or more, the length of collecting duct is within 1m.
The packing method of embodiment 5 Amblyseius persidolongispinosus
After utilizing collection device to collect Amblyseius persidolongispinosus, can directly pack the when of also can first refrigerating to be needed and pack again,
Packing method is by packaging standard amount, Amblyseius persidolongispinosus is put into the Packaging Bottle with dry or wet vermiculite, the most quickly covers tightly
Bottle cap, it is desirable to Packaging Bottle body and bottle cap can fully seal, can effectively stop mite class to be escaped, and Amblyseius persidolongispinosus has been packed
After can apply or continue refrigeration, refrigerated storage temperature is at 0 DEG C-20 DEG C, preferably 3 DEG C-8 DEG C, and described Packaging Bottle bottle cap is with ventilative
Hole, air-vent size, shape do not fix, but air-vent need to be sealed by paper or gauze, gauze, cloth, is preferably sealed by paper.
The process of embodiment 6 planting matrix and re-using
First in tetranychid or Amblyseius persidolongispinosus receptacle, remove used planting matrix, it is possible to the plantation utensil being used in connection with
Removing receptacle together, after removing or pour out used planting matrix, place more than 0.1 day, used planting matrix is treated
Being continuing with after directly sieving or smash with pulverizer after reaching certain mass dryness fraction, if wherein sieving, screen sizes is 0.1cm-8cm,
Screen cloth can allow planting matrix to pass through and can prevent host plant root, stem, leaf or other foreign material from passing through, the root, stem and leaf of host plant
Can be continuing with after smashing in pulverizer or directly abandon, the planting matrix stayed after sieving or after smashing can directly utilize or with
New planting matrix is used in mixed way or adds other composition being conducive to host plant to grow, but is utilizing these used planting matrixs
Preferably through freezing, high temperature deinsectization sterilizing or process of being exposed to the sun before matter, there is no Amblyseius persidolongispinosus, tetranychid in guaranteeing planting matrix
Or other insects, pathogenic bacteria etc..Wherein, described plantation utensil refers to for planting the basin of host plant, dish, container etc., this
Being filled with planting matrix in a little utensils, host plant is grown in these planting matrixs.
Claims (11)
1. a method for a large amount of production Amblyseius persidolongispinosus, is characterized in that raising tetranychid, then profit first with the host plant of tetranychid
The method raising Amblyseius persidolongispinosus with the tetranychid raised, concrete key operation step is:
1) raising of tetranychid: in host plant launches the first 0.5 day-15 days to true leaf, by the blade with tetranychid kind source
Or plants stems is inoculated on host plant together with the composition of blade, the tetranychid in inoculation is each mite state of tetranychid, comprises children mite,
Described blade is not cut into small pieces or is seldom cut into small pieces, and wherein host plant width of blade is at more than 1cm, and average every strain host plants
The tetranychid amount that thing accesses, more than 5, is removed the blade inoculated for host plant or plants in 0.1 day-10 days after inoculation
Thing stem and the composition of blade, wherein the terminal bud to plant processes is not cut or cut less；
2) raising of Amblyseius persidolongispinosus: the whole receptacle raising Amblyseius persidolongispinosus is to seal, but can breathe freely, and is raising
During supporting Amblyseius persidolongispinosus, it is not necessary to dependent insect cage,
The method for breeding first of Amblyseius persidolongispinosus: during raising Amblyseius persidolongispinosus, the inoculation of Amblyseius persidolongispinosus carries
Body is not essential, if having, inoculation carrier is the most singly blade, for not being cut into as the blade of Amblyseius persidolongispinosus inoculation carrier
Fritter or be seldom cut into small pieces；Amblyseius persidolongispinosus each mite state, tetranychid and plan is accessed when every strain tetranychid amount reaches more than 100
The access ratio of amblyseius as predatory mite of becoming mildewed is more than 5: 1, and processing the terminal bud of plant is not cut or cut less；
Next batch and the method raising Amblyseius persidolongispinosus the most continuously, method for breeding is raised first with above-mentioned Amblyseius persidolongispinosus
Breeding method is identical, or selects following method for breeding, and concrete operations are: after last batch Amblyseius persidolongispinosus is gathered, and new is a collection of
Host plant with tetranychid, Amblyseius persidolongispinosus to be seeded is not to continue with using inoculation Amblyseius persidolongispinosus to host plant
Method mass rearing Amblyseius persidolongispinosus, but after last batch Amblyseius persidolongispinosus is gathered within 10 days, to be seeded is posted
Main plant moves in the receptacle of the complete Amblyseius persidolongispinosus of freshly harvested, is placed on raising frame, raises platform, ground, the complete plan of freshly harvested
The amblyseius as predatory mite that becomes mildewed remaining host plant plantation utensil, planting matrix or other can see that each of Amblyseius persidolongispinosus is local, 0.1
My god remove the host plant plantation utensil that last batch stays after-10 days, the most again put or shift the host plant stayed,
Determine whether directly to gather according to the amount of Amblyseius persidolongispinosus on host plant or mend again fill out part with tetranychid host plant,
Or continue to raise or mend again to put continuation raising after part Amblyseius persidolongispinosus；
3) method that separates and collects of Amblyseius persidolongispinosus: when the Amblyseius persidolongispinosus number of animals raised reaches the amount of gathering, it becomes possible to intending becoming mildewed
Amblyseius as predatory mite carries out gathering, collecting, and collection method is to cut host plant with scissors, then plants stems, branch is put into together with leaf
In collection device, or the blade of independent clip host plant or branches and leaves are put in collection device, seal collection device the most as early as possible,
Again collection device is placed in suitable space, fixes the collection that i.e. can be used for Amblyseius persidolongispinosus each movable mite state after putting well, receive
Collection operation is every weekly check, collects more than 1 time, and till collecting pipe is collected less than Amblyseius persidolongispinosus, collecting pipe is length
Within 1m, for collecting the container of Amblyseius persidolongispinosus, it is ensured that collect less than clearing up collection device after Amblyseius persidolongispinosus
Interior plants stems, branch or leaf；
4) packing method of Amblyseius persidolongispinosus: after utilizing collection device to collect Amblyseius persidolongispinosus, can directly pack and also may be used
Packing the when of first refrigerating to be needed, packing method is by packaging standard amount, puts into Amblyseius persidolongispinosus with vermiculite or wood again
In the Packaging Bottle of bits or vermiculite and wood chip mixture, the most quickly cover tightly bottle cap, it is desirable to Packaging Bottle body and bottle cap can be the closeest
Sealing, can effectively stop mite class to be escaped, Amblyseius persidolongispinosus can apply or continue refrigeration after having packed, refrigerated storage temperature is at 0 DEG C
-20 DEG C, described Packaging Bottle bottle cap is not fixed with air-vent, air-vent size, shape, but air-vent need to be by paper or gauze, cloth
Seal；
5) process of planting matrix and re-using: first remove used planting matrix in tetranychid or Amblyseius persidolongispinosus receptacle
Matter, or the plantation utensil being used in connection with removes receptacle together, after removing or pour out used planting matrix, places 0.1
More than it, it is continuing with after directly sieving or smash with pulverizer after used planting matrix certain mass dryness fraction to be achieved, Qi Zhongruo
Sieving, screen cloth mesh length of side size is 0.1cm-10cm, screen cloth planting matrix can be allowed to pass through and can prevent host plant root, stem,
Leaf or other foreign material pass through, and the root, stem and leaf of host plant is continuing with after smashing in pulverizer or directly abandons, after sieving or beat
The planting matrix stayed after broken can directly utilize or be used in mixed way with new planting matrix or add other and be conducive to host plant to grow
Composition, but will be through freezing, high temperature deinsectization sterilizing or process of being exposed to the sun before utilizing these used planting matrixs, to guarantee to plant
Plant Medium Culture and there is no Amblyseius persidolongispinosus, tetranychid or other insects, pathogenic bacteria.
A kind of method of a large amount of production Amblyseius persidolongispinosus, is characterized in that described plants stems and blade
Composition refer to the most singly to have in the carrier that tetranychid or Amblyseius persidolongispinosus are inoculated blade also have the side shoot on plants stems or stem,
Branch, palpus branch.
A kind of method of a large amount of production Amblyseius persidolongispinosus, is characterized in that described blade is seldom cut into
Fritter refers to that blade is completely or partially complete, and incomplete part is to shear planting with tetranychid or Amblyseius persidolongispinosus
During strain, may incidentally miscut or accidentally injure the blade fritter that formed of blade.
A kind of method of a large amount of production Amblyseius persidolongispinosus, it is characterized in that described terminal bud processes is not
Cut or cut less and refer to not cut terminal bud or cut terminal bud rather than always once find that terminal bud is wiped out the most in time several times less, need to protect
Stay part terminal bud or all terminal bud continued growths.
A kind of method of a large amount of production Amblyseius persidolongispinosus, is characterized in that described Amblyseius persidolongispinosus
Inoculation not essential the referring to of carrier can inoculate the pure Amblyseius persidolongispinosus without carrier, it is also possible to inoculate long with the plan of carrier
Hair amblyseius as predatory mite.
A kind of method of a large amount of production Amblyseius persidolongispinosus, it is characterized in that described in single simply blade
Refer to that the inoculation carrier of Amblyseius persidolongispinosus is the carrier of any carrying Amblyseius persidolongispinosus.
A kind of method of a large amount of production Amblyseius persidolongispinosus, is characterized in that described plantation utensil refers to
For planting the container of host plant, being filled with planting matrix in these utensils, host plant is grown in these planting matrixs.
A kind of method of a large amount of production Amblyseius persidolongispinosus, is characterized in that described collection device refers to
For collecting the utensil of Amblyseius persidolongispinosus, collection device can be made up of two parts and also can be made up of three parts, if collection device by
Two parts composition then includes collecting container and collecting pipe two parts, and when collecting Amblyseius persidolongispinosus, collecting pipe directly and collects container
Being connected, Amblyseius persidolongispinosus is directly to climb in collecting pipe in collecting container；If collection device is made up of three parts, including receiving
Collection container, be contained in the collecting duct and collecting pipe three part collected on container, when collecting Amblyseius persidolongispinosus, collecting pipe directly with
Collecting duct is connected, Amblyseius persidolongispinosus be from collect in container first climb to collecting duct in climb to again in collecting pipe.
A kind of method of a large amount of production Amblyseius persidolongispinosus, is characterized in that described collection device is placed on
Suitably space refers to be designed for the space that Amblyseius persidolongispinosus is collected, it is desirable to there is light in this space when Amblyseius persidolongispinosus is collected
Unglazed, but need when changing plants stems, branch or the leaf in collecting pipe, cleaning collection device in fixing collection device, inspection
There is light.
A kind of method of a large amount of production Amblyseius persidolongispinosus, collects container and refers to for loading plan
Become mildewed amblyseius as predatory mite and the part of plants stems, branch or leaf, collects container and has 0.001m3-1m3Volume, collect container manufacture
Material must contain gauze, cloth, fly net, paper or other material can breathed freely and can effectively stop mite class to be escaped, described gauze,
Cloth, fly net, paper or other can be breathed freely and the material that can effectively stop mite class to be escaped is used alone or two or more is used in mixed way,
Collect container and must also have preferable sealing, it is possible to ensure that Amblyseius persidolongispinosus cannot be from addition to climbing in collecting pipe as far as possible
Other place climbs out of, collect container also there is the opening being easily opened and sealing, open opening can load Amblyseius persidolongispinosus and
Plants stems, branch or leaf, seal opening the most salable collection container, wherein collect on container and be used for loading Amblyseius persidolongispinosus and planting
The opening that strain stem, branch, the opening of leaf climb out of in collecting container more than Amblyseius persidolongispinosus.
The method of 11. a kind of a large amount of production Amblyseius persidolongispinosus, is characterized in that described collecting duct is
Referring to be contained in and collect the passage climbed out of for Amblyseius persidolongispinosus on container, Amblyseius persidolongispinosus can be contained in collection from collecting duct to be held
That on device climbs out of collection container, then connect together from collecting duct and collecting pipe that crawl into collecting pipe, collect and hold
Collecting duct number on device is one or more, and the length of collecting duct is within 1m.
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| EP2042036B1 (en) * | 2004-12-31 | 2011-06-22 | Koppert B.V. | Mite composition, use thereof, method for rearing the phytoseiid predatory mite Amblyseius swirskii, rearing system for rearing said phytoseiid mite and methods for biological pest control on a crop |
| US7947269B2 (en) * | 2004-12-31 | 2011-05-24 | Koppert B.V. | Mite composition, use thereof, method for rearing a phytoseiid predatory mite, rearing system for rearing said phytoseiid predatory mite and methods for biological pest control on a crop |
| CN101040612A (en) * | 2007-04-09 | 2007-09-26 | 张艳璇 | Production process of predacious mite |
| CN101268772B (en) * | 2008-05-23 | 2012-03-21 | 北京依科曼生物技术有限公司 | Predatory mites producing method |
| CN101288392A (en) * | 2008-06-19 | 2008-10-22 | 张艳璇 | Artificial feeding production method of Neoseiulus |
| CN101331869A (en) * | 2008-08-07 | 2008-12-31 | 北京市农林科学院 | Chile phytoseiulus persimilis propagating method |
| CN101773099B (en) * | 2010-01-29 | 2013-11-13 | 张艳璇 | Method for artificial feeding production of Steinman small blind cucumeris |
| CN102742554A (en) * | 2012-07-06 | 2012-10-24 | 贵州大学 | Artificial raising method for euseius nicholsi |
| CN103053477B (en) * | 2012-12-26 | 2014-01-22 | 中国农业科学院郑州果树研究所 | Scale breeding method of Scolothrips takahashii |
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