CN104221827B - A kind of candidum tissue culturing seedling directly transplants soilless planting method - Google Patents
A kind of candidum tissue culturing seedling directly transplants soilless planting method Download PDFInfo
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- CN104221827B CN104221827B CN201310221835.5A CN201310221835A CN104221827B CN 104221827 B CN104221827 B CN 104221827B CN 201310221835 A CN201310221835 A CN 201310221835A CN 104221827 B CN104221827 B CN 104221827B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The present invention discloses a kind of candidum tissue culturing ground and directly transplants soilless planting method, its Technology step: tissue cultured seedling is transplanted front and back and cultivated in plastic greenhouse by tissue-culture container seedling, the transplanting of tissue cultured seedling, maintenance management after transplanting, the prevention and control of plant diseases, pest control, tissue cultured seedling is transplanted plantation about a year or is grown into the harvesting after more than 20 centimetres; Surviving rate height, growth is fast, output height, and disease and pest is few, and contained by Herba Dendrobii, major ingredient is guaranteed, and greatly reduce plantation and handling cost, it is to increase the economic benefit of plantation.
Description
Technical field
The present invention relates to a kind of candidum tissue culturing seedling and directly transplant soilless planting method, belong to Chinese medicinal materials cultivation hydroponics technical field.
Background technology
The stem of noble dendrobium is Chinese medicinal materials, and its kind is more, and Herba Dendrobii is one of main famous and precious kind that " Chinese Pharmacopoeia " records, pharmaceutical use height, and nourishing function is strong, and major ingredient is the trace elements such as polysaccharide, dendrobine, amino acid. effects such as there is enriching yin clearing lung-heat, reinforcing stomach reg fluid, the clear bright order of throat, strengthen body immunity, be anti-ageing, antitumor. for many years, owing to producing region medicinal herb grower's destructiveness is excavated, make dendrobium officinale resource endangered, Herba Dendrobii has been classified as and has laid special stress on protecting rare and endangered species by country, and encourage to reward domestic artificial group of training and artificial growth Herba Dendrobii, become that wild harvesting is artificial propagation, plantation, to ensure sustainable use and the development of Herba Dendrobii resource. Herba Dendrobii breeding planting technology research originates in 20th century the seventies, main contents have: candidum tissue culturing seedling is bred fast, hardening domestication research, group training and hardening acclimatization technology are comparatively ripe, develop also very fast, but the artificial establishing in large scale of Herba Dendrobii is still in the starting stage in China, not on a large scale, fltting speed is slower, wherein major cause is that tissue cultured seedling transplanting survival rate is low, what be more a problem is, the hardening acclimatization technology technique that employing Cortex Pini makes matrix is immature, transplant growth conditions difference too big, it is long to there is the cultivation plantation cycle in these technology, the shortcomings such as cost height, therefore, how to shorten tissue cultured seedling to cultivate and the plantation cycle becomes Herba Dendrobii to realize the plantation of artificial large-scale planting crucial.
Summary of the invention
It is an object of the invention to overcome the defect of prior art processes existence and make improvements, it is provided that a kind of candidum tissue culturing seedling directly transplants soilless planting method.
It is characterized in that: a kind of candidum tissue culturing seedling directly transplants soilless planting method, through following Technology step:
Tissue-culture container seedling is utilized natural light before transplanting by A, tissue cultured seedling in plastic greenhouse, after adapting to 5-10 days, can transplant in plastics planting tray by bottle outlet under being allowed to condition at natural light;
A. in pond, plastics planting tray and funnel-form plantation casket are sterilized more than 2 hours together with clear water of sterilizing, in tissue cultured seedling bottle, pour into clear water of sterilizing on a small quantity in addition;
B. press from both sides the base portion toward plant in tissue cultured seedling bottle with chopsticks or metal tweezers, plant is pulled out gently tissue cultured seedling bottle, puts into the pond sterilized, sterilize under the prerequisite not injuring stem, leaf, root system, and clean root system;
C. placing funnel-form plantation casket in the grid of falling funnel-form on plastics planting tray upper strata, the funnel-form plantation lower end of casket immerses in the aqueous stratum of plastics planting trays lower floor, and aqueous stratum height is 8-12 centimetre;
D. implanting in funnel-form plantation casket through cleaned plant 4-6 strain, the root system of plant immerses in aqueous stratum;
Maintenance management after the plantation of B, tissue cultured seedling;
A. tissue cultured seedling forms plant after being transplanted to plastics planting tray, plastics planting tray is placed in pattern plastic warmhouse booth overhead highly at 50-80 centimetre, in pattern plastic warmhouse booth, temperature controls between 5-30 DEG C, and illumination controls between 3000-5000lux, and in canopy, relative air humidity is no more than 60%;
B. after tissue cultured seedling is transplanted to plastics planting tray, the most junior three is poured water three times in 24 hours month every days at regular time and quantity, pours water lasting 1 hour every time, and other times are in anhydrous state, and per week adopts liquid nutrient solution to apply fertilizer once, each 1.5 ~ 2.5 hours;
C. after plant is after three months or it grows into 5-7 cm long, pour water at regular time and quantity in 24 hours every days 2 times, each 1 hour, other times are in anhydrous state, per week adopts liquid nutrient solution to apply fertilizer 2 times, each 1.5 ~ 2.5 hours, daytime every day foliar spray 3-4 time, at night foliar spray 1 time;
C, the prevention and control of plant diseases, pest control: the prevention and control of plant diseases, pest control should be followed and put prevention first, usually it is noted that observe disease or insect pest, once discovery disease and pest symptom, spray with low-toxin farm chemicals, per week medication 1 time, and the spray medicine time is between afternoon 4-6 point;
D, when tissue cultured seedling transplant plantation year and a day, or grow into more than 20 centimetres, can pluck, with the scissors of volumetric concentration more than 80% alcohol disinfecting from Herba Dendrobii root 1 centimeters horizontal shear before plucking, spray water after shearing 1 hour once, branch bar under shearing can be used as fresh goods listing, or is processed into dry product sale;
Described liquid nutrient solution can be high nitrogenous fertilizer or balance fertilizer;
The described humidity in plastics planting tray remains on more than 80%.
Implementing the obvious advantage after this technology and effect is: surviving rate height, growth is fast, output height, and disease and pest is few, and contained by Herba Dendrobii, major ingredient is guaranteed, and greatly reduce plantation and handling cost, it is to increase the economic benefit of plantation.
Embodiment
Hereinafter being used in each funnel-form plantation casket, to be implanted into 5 strains be example, and the invention will be further described:
Tissue-culture container seedling is utilized natural light before and after transplanting by A, tissue cultured seedling in plastic greenhouse, after adapting to 8 days, can transplant in plastics planting tray by bottle outlet under being allowed to condition at natural light;
A. in pond, plastics planting tray and funnel-form plantation casket are sterilized more than 2 hours together with clear water of sterilizing, in tissue cultured seedling bottle, pour into clear water of sterilizing on a small quantity in addition;
B. press from both sides the base portion toward plant in tissue cultured seedling bottle with chopsticks or metal tweezers, plant is pulled out gently tissue culture bottle, puts into the pond that poison is crossed clearly, sterilize under the prerequisite not injuring stem, leaf, root system, and clean root system;
C. placing funnel-form plantation casket in the grid of falling funnel-form on plastics planting tray upper strata, the funnel-form plantation lower end of casket immerses in the aqueous stratum of plastics planting trays lower floor, and aqueous stratum height is 10 centimetres;
D. implanting in funnel-form plantation casket through cleaned plant 5 strain, the root system of plant immerses in aqueous stratum;
Maintenance management after the plantation of B, tissue cultured seedling;
A. tissue cultured seedling forms plant after being transplanted to plastics planting tray, plastics planting tray is placed in pattern plastic warmhouse booth overhead highly at 60 centimetres, in pattern plastic warmhouse booth, temperature controls between 25 DEG C, and illumination controls between 4000lux �� 500, and in canopy, relative air humidity is no more than 60%; Humidity in plastics planting tray remains on more than 80%;
B. after tissue cultured seedling is transplanted to plastics planting tray, the most junior three was poured water once every 8 hours in 24 hours month every days, poured water lasting 1 hour every time, and other times are in anhydrous state, and per week adopts liquid nutrient solution to apply fertilizer once, each 2 hours;
C., after plant is after three months or it grows into 5 cm long, pour water in 12 hours every days once, each 1 hour, other times are in anhydrous state, and per week adopts high nitrogenous fertilizer liquid or other nutritive mediums to apply fertilizer 2 times, each 2 hours, daytime every day foliar spray 3 times, at night foliar spray 1 time;
C, the prevention and control of plant diseases, pest control: the prevention and control of plant diseases, pest control should be followed and put prevention first, usually it is noted that observe disease or insect pest, once discovery disease and pest symptom, spray with low-toxin farm chemicals, per week medication 1 time, if desired, per week 3 times, the spray medicine time is between afternoon 4-6 point;
D, when tissue cultured seedling transplant plantation year and a day about, or grow into more than 20 centimetres, can pluck, with the scissors of volumetric concentration more than 80% alcohol disinfecting from Herba Dendrobii root 1 centimeters horizontal shear before plucking, spray water after shearing 1 hour once, branch bar under shearing can be used as fresh goods listing, or is processed into dry product sale.
Claims (1)
1. candidum tissue culturing seedling directly transplants a soilless planting method, through following Technology step:
Tissue-culture container seedling is utilized natural light before transplanting by A, tissue cultured seedling in plastic greenhouse, after adapting to 5-10 days, can transplant in plastics planting tray by bottle outlet under being allowed to condition at natural light;
A. in pond, plastics planting tray and funnel-form plantation casket are sterilized more than 2 hours together with clear water of sterilizing, in tissue cultured seedling bottle, pour into clear water of sterilizing on a small quantity in addition;
B. press from both sides the base portion toward plant in tissue cultured seedling bottle with chopsticks or metal tweezers, plant is pulled out gently tissue cultured seedling bottle, puts into the pond sterilized, sterilize under the prerequisite not injuring stem, leaf, root system, and clean root system;
C. placing funnel-form plantation casket in the grid of falling funnel-form on plastics planting tray upper strata, the funnel-form plantation lower end of casket immerses in the aqueous stratum of plastics planting trays lower floor, and aqueous stratum height is 8-12 centimetre;
D. implanting in funnel-form plantation casket through cleaned plant 4-6 strain, the root system of plant immerses in aqueous stratum;
Maintenance management after the plantation of B, tissue cultured seedling;
A. tissue cultured seedling forms plant after being transplanted to plastics planting tray, plastics planting tray is placed in pattern plastic warmhouse booth overhead highly at 50-80 centimetre, in pattern plastic warmhouse booth, temperature controls between 5-30 DEG C, and illumination controls between 3000-5000lux, and in canopy, relative air humidity is no more than 60%;
B. after tissue cultured seedling is transplanted to plastics planting tray, the most junior three is poured water three times in 24 hours month every days at regular time and quantity, pours water lasting 1 hour every time, and other times are in anhydrous state, and per week adopts liquid nutrient solution to apply fertilizer once, each 1.5 ~ 2.5 hours;
C. after plant is after three months or it grows into 5-7 cm long, pour water at regular time and quantity in 24 hours every days 2 times, each 1 hour, other times are in anhydrous state, per week adopts liquid nutrient solution to apply fertilizer 2 times, each 1.5 ~ 2.5 hours, daytime every day foliar spray 3-4 time, at night foliar spray 1 time;
C, the prevention and control of plant diseases, pest control: the prevention and control of plant diseases, pest control should be followed and put prevention first, usually it is noted that observe disease or insect pest, once discovery disease and pest symptom, spray with low-toxin farm chemicals, per week medication 1 time, and the spray medicine time is between afternoon 4-6 point;
D, when tissue cultured seedling transplant plantation year and a day, or grow into more than 20 centimetres, can pluck, with the scissors of volumetric concentration more than 80% alcohol disinfecting from Herba Dendrobii root 1 centimeters horizontal shear before plucking, spray water after shearing 1 hour once, branch bar under shearing can be used as fresh goods listing, or is processed into dry product sale.
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| CN104221827B true CN104221827B (en) | 2016-06-08 |
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Families Citing this family (8)
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| CN104663395B (en) * | 2015-02-04 | 2017-01-18 | 郴州市芝草农业科技开发有限公司 | Process for manufacturing dendrobium officinale |
| CN104838998A (en) * | 2015-06-03 | 2015-08-19 | 饶平茂丰源生物科技有限公司 | Under-forest dendrobium candidum soilless stereoscopic planting method |
| CN105532396A (en) * | 2015-12-09 | 2016-05-04 | 安徽和合园林绿化工程有限公司 | Method for culturing persimmon tree seedlings by using culture medium |
| CN105454016A (en) * | 2015-12-09 | 2016-04-06 | 安徽和合园林绿化工程有限公司 | Method for culturing silver birch seedling by culture medium |
| CN105454017A (en) * | 2015-12-09 | 2016-04-06 | 安徽和合园林绿化工程有限公司 | Method for culturing maple seedling by culture medium |
| CN105454014A (en) * | 2015-12-09 | 2016-04-06 | 安徽和合园林绿化工程有限公司 | Method for culturing bischofia polycarpa seedling by culture medium |
| CN105454015A (en) * | 2015-12-09 | 2016-04-06 | 安徽和合园林绿化工程有限公司 | Method for culturing willow seedling by culture medium |
| CN106386440A (en) * | 2016-09-05 | 2017-02-15 | 深圳市双赢伟业科技股份有限公司 | Standardized soilless cultivation method and standardized soilless cultivation method for traditional Chinese medicine |
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| CN102422811A (en) * | 2011-10-06 | 2012-04-25 | 红河州巨丰生物科技有限公司 | Dendrobium officinale tissue culture reproduction method |
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| EP1704769A1 (en) * | 2003-12-26 | 2006-09-27 | Noriko Takeda | Method of growing plant, water freshness preserver, root rot inhibitor, plant medium, water holder, bactericide, herbicide, heat insulator, improver, deodorizer and water-freshness preservation |
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