CN104204898B - 光片光学系统 - Google Patents

光片光学系统 Download PDF

Info

Publication number
CN104204898B
CN104204898B CN201380018625.4A CN201380018625A CN104204898B CN 104204898 B CN104204898 B CN 104204898B CN 201380018625 A CN201380018625 A CN 201380018625A CN 104204898 B CN104204898 B CN 104204898B
Authority
CN
China
Prior art keywords
mating plate
optical system
light
asymmetric
plate optical
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201380018625.4A
Other languages
English (en)
Chinese (zh)
Other versions
CN104204898A (zh
Inventor
K·德拉起亚
T·威滕伯格
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of St Andrews
Original Assignee
University of St Andrews
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1205974.7A external-priority patent/GB201205974D0/en
Priority claimed from GBGB1215169.2A external-priority patent/GB201215169D0/en
Application filed by University of St Andrews filed Critical University of St Andrews
Publication of CN104204898A publication Critical patent/CN104204898A/zh
Application granted granted Critical
Publication of CN104204898B publication Critical patent/CN104204898B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/10—Condensers affording dark-field illumination
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0927—Systems for changing the beam intensity distribution, e.g. Gaussian to top-hat
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0938—Using specific optical elements
    • G02B27/0944—Diffractive optical elements, e.g. gratings, holograms
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0938—Using specific optical elements
    • G02B27/0988—Diaphragms, spatial filters, masks for removing or filtering a part of the beam
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N7/00—Television systems
    • H04N7/18—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Signal Processing (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Nonlinear Science (AREA)
  • Optical Elements Other Than Lenses (AREA)
  • Tents Or Canopies (AREA)
CN201380018625.4A 2012-04-03 2013-03-26 光片光学系统 Active CN104204898B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB1205974.7A GB201205974D0 (en) 2012-04-03 2012-04-03 High resolution imaging of extended volumes
GB1205974.7 2012-04-03
GBGB1215169.2A GB201215169D0 (en) 2012-08-24 2012-08-24 High resolution imaging of extended volumes
GB1215169.2 2012-08-24
PCT/GB2013/050788 WO2013150273A1 (en) 2012-04-03 2013-03-26 High resolution imaging of extended volumes

Publications (2)

Publication Number Publication Date
CN104204898A CN104204898A (zh) 2014-12-10
CN104204898B true CN104204898B (zh) 2017-12-15

Family

ID=48095913

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380018625.4A Active CN104204898B (zh) 2012-04-03 2013-03-26 光片光学系统

Country Status (8)

Country Link
US (1) US10444520B2 (enExample)
EP (1) EP2834695B1 (enExample)
JP (1) JP6166776B2 (enExample)
CN (1) CN104204898B (enExample)
CA (1) CA2869359C (enExample)
ES (1) ES2898616T3 (enExample)
IN (1) IN2014DN09127A (enExample)
WO (1) WO2013150273A1 (enExample)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7838302B2 (en) 2006-08-07 2010-11-23 President And Fellows Of Harvard College Sub-diffraction limit image resolution and other imaging techniques
CN101918816B (zh) 2007-12-21 2015-12-02 哈佛大学 三维中的亚衍射极限图像分辨率
US9404869B2 (en) 2012-10-09 2016-08-02 Howard Hughes Medical Institute Multiview light-sheet microscopy
DE102013208926A1 (de) * 2013-05-14 2014-11-20 Carl Zeiss Microscopy Gmbh Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie
US10539772B2 (en) 2013-10-09 2020-01-21 Howard Hughes Medical Institute Multiview light-sheet microscopy
DE102013112595B4 (de) * 2013-11-15 2024-08-01 Carl Zeiss Microscopy Gmbh Anordnung zur Lichtblattmikroskopie
DE102013021222B4 (de) * 2013-12-17 2023-05-04 Carl Zeiss Microscopy Gmbh Mikroskop und Mikroskopieverfahren
WO2015117115A1 (en) * 2014-02-03 2015-08-06 President And Fellows Of Harvard College Three-dimensional super-resolution fluorescence imaging using airy beams and other techniques
DE102014102215A1 (de) * 2014-02-20 2015-08-20 Carl Zeiss Microscopy Gmbh Verfahren und Anordnung zur Lichtblattmikroskopie
GB201413500D0 (en) 2014-07-30 2014-09-10 Univ St Andrews Airy beam light sheet
DE102014113827A1 (de) * 2014-09-24 2016-03-24 Carl Zeiss Microscopy Gmbh Vorrichtung zur Abbildung einer Probe
CN104459971B (zh) * 2014-12-24 2016-01-13 中国科学院半导体研究所 一种基于集成光子芯片的结构光照明显微成像系统
GB201508376D0 (en) * 2015-05-15 2015-07-01 Univ St Andrews Light sheet imaging
US10942346B2 (en) 2015-05-22 2021-03-09 The Hong Kong University Of Science And Technology Methods and systems for generating non-diffracting light sheets for multicolor fluorescence microscopy
DE102015209758A1 (de) * 2015-05-28 2016-12-01 Carl Zeiss Microscopy Gmbh Anordnung und Verfahren zur Strahlformung und zur Lichtblattmikroskopie
EP3341710B1 (en) * 2015-08-26 2022-02-16 The United States of America, as represented by The Secretary, Department of Health & Human Services Systems and methods for multi-view nonlinear optical imaging for improved signal-to-noise ratio and resolution in point scanning multi-photon microscopy
DE102016203671A1 (de) * 2016-03-07 2017-09-07 Carl Zeiss Microscopy Gmbh Verfahren zum Bestimmen einer Höheninformationen einer Probe und Scanningmikroskop
DE102016108384B3 (de) 2016-05-04 2017-11-09 Leica Microsystems Cms Gmbh Vorrichtung und Verfahren zur lichtblattartigen Beleuchtung einer Probe
CN105866013A (zh) * 2016-05-26 2016-08-17 天津大学 基于两幅激光干涉成像离焦干涉图的球形粒子判别方法
US11320640B2 (en) 2016-06-24 2022-05-03 Howard Hughes Medical Institute Automated adjustment of light sheet geometry in a microscope
CN106053346B (zh) * 2016-06-29 2018-06-08 华中科技大学 一种光片显微成像转换装置
CN106153307B (zh) * 2016-06-30 2018-09-28 河南科技大学 一种利用光强比测量Airy光束衰减因子的装置和方法
EP3494419A4 (en) * 2016-08-07 2020-03-18 Ramot at Tel-Aviv University Ltd. METHOD AND SYSTEM FOR IMAGING AN INTERNAL MEDIUM
EP3497502B1 (de) * 2016-08-15 2022-10-26 Leica Microsystems CMS GmbH Lichtblattmikroskop
CN107991766A (zh) 2016-10-26 2018-05-04 中国科学技术大学 一种具有三维成像能力的显微镜和成像方法
US11506877B2 (en) 2016-11-10 2022-11-22 The Trustees Of Columbia University In The City Of New York Imaging instrument having objective axis and light sheet or light beam projector axis intersecting at less than 90 degrees
US20180139366A1 (en) * 2016-11-11 2018-05-17 Cold Spring Harbor Laboratory System and method for light sheet microscope and clearing for tracing
SG11201909955XA (en) * 2017-05-02 2019-11-28 Merck Sharp & Dohme Formulations of anti-lag3 antibodies and co-formulations of anti-lag3 antibodies and anti-pd-1 antibodies
CN107238590A (zh) * 2017-05-24 2017-10-10 清华大学 基于光片显微与单像素成像的显微层析成像装置
CN107144951B (zh) * 2017-06-30 2023-03-21 中国计量大学 一种基于半球微结构的超分辨显微装置
CN108303402A (zh) * 2017-12-26 2018-07-20 华中科技大学 一种大视场无衍射贝塞尔光片显微扫描成像方法及系统
WO2019135069A1 (en) * 2018-01-02 2019-07-11 King's College London Method and system for localisation microscopy
CN108398774B (zh) * 2018-01-18 2021-03-02 中国科学院广州生物医药与健康研究院 一种光片显微镜
WO2019153530A1 (zh) * 2018-02-06 2019-08-15 西安电子科技大学 一种双模态拉曼-光学投影断层成像系统
CN108469429B (zh) * 2018-02-06 2020-11-24 西安电子科技大学 一种双模态拉曼-光学投影断层成像系统
US10247910B1 (en) 2018-03-14 2019-04-02 Nanotronics Imaging, Inc. Systems, devices and methods for automatic microscopic focus
US10146041B1 (en) 2018-05-01 2018-12-04 Nanotronics Imaging, Inc. Systems, devices and methods for automatic microscope focus
CN108732124B (zh) * 2018-05-21 2023-05-26 深圳市重投华讯太赫兹科技有限公司 一种三维层析成像系统及方法
CN108956562B (zh) * 2018-06-11 2020-05-22 浙江大学 一种基于重定位的光切片荧光显微成像方法和装置
CN108645831B (zh) * 2018-06-14 2020-11-20 厦门大学 多功能表面等离子体耦合发射荧光与拉曼检测仪及其检测方法
DE102018115001A1 (de) * 2018-06-21 2019-12-24 Carl Zeiss Microscopy Gmbh Verfahren zum Kalibrieren einer Phasenmaske und Mikroskop
EP3620841A1 (en) * 2018-09-07 2020-03-11 ETH Zurich Automated system for wide-field multiphoton microscope
CN109297949B (zh) * 2018-09-19 2024-04-05 上海镭立激光科技有限公司 显微影像结合透射拉曼光谱的肿瘤细胞检测方法及装置
EP3876990A4 (en) 2018-11-07 2023-09-06 Merck Sharp & Dohme LLC CO-FORMULATIONS OF ANTI-LAG3 ANTIBODIES AND ANTI-PD-1 ANTIBODIES
CN109656028A (zh) * 2019-01-11 2019-04-19 中国科学院广州生物医药与健康研究院 一种产生无衍射光的系统及方法
US11187659B2 (en) * 2019-03-28 2021-11-30 The Regents Of The University Of California Systems for fluorescence light sheet microscopy of large samples in high refractive index solutions
US20220196557A1 (en) * 2019-04-05 2022-06-23 Cytoveris Inc. Angular depth resolved raman spectroscopy apparatus and method
CN110441983B (zh) * 2019-07-24 2022-09-09 成都仲伯科技有限公司 基于光学传递函数的x光高分辨率成像方法
US11940611B2 (en) 2020-03-06 2024-03-26 Georgia Tech Research Corporation Tomographic imaging systems and methods
CN112821946A (zh) * 2021-01-06 2021-05-18 广东工业大学 一种基于自加速光束的短距自由空间光互连系统
CN113075174B (zh) * 2021-03-12 2022-07-05 华中科技大学 一种斜置上顶式静态贝塞尔光片成像系统
CN115308917B (zh) * 2022-08-23 2025-07-11 浙江理工大学 一种矢量光场模间相位旋转角度随距离可调系统
SE546014C2 (en) * 2022-09-30 2024-04-16 Spec Imaging Ab An assembly for measurements of one or more optical parameters of a medium and a method of using the assembly

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1667494A (zh) * 2004-03-08 2005-09-14 明基电通股份有限公司 图像显示装置
WO2006058187A2 (en) * 2004-11-23 2006-06-01 Robert Eric Betzig Optical lattice microscopy
DE102007063274B8 (de) * 2007-12-20 2022-12-15 Albert-Ludwigs-Universität Freiburg Mikroskop
WO2012003259A1 (en) * 2010-06-30 2012-01-05 The Government Of The U.S.A., Represented By The Secretary, Department Of Health And Human Services System and method of producing nondiffracting light sheets by a multiplicity of spatially overlapping, minimally interfering nondiffracting optical beams

Also Published As

Publication number Publication date
US10444520B2 (en) 2019-10-15
US20150029325A1 (en) 2015-01-29
WO2013150273A1 (en) 2013-10-10
EP2834695B1 (en) 2021-08-25
JP6166776B2 (ja) 2017-07-19
JP2015514235A (ja) 2015-05-18
CA2869359A1 (en) 2013-10-10
CA2869359C (en) 2021-03-23
ES2898616T3 (es) 2022-03-08
EP2834695A1 (en) 2015-02-11
CN104204898A (zh) 2014-12-10
IN2014DN09127A (enExample) 2015-05-22

Similar Documents

Publication Publication Date Title
CN104204898B (zh) 光片光学系统
Jiang et al. Resolution-enhanced parallel coded ptychography for high-throughput optical imaging
Olarte et al. Light-sheet microscopy: a tutorial
Booth Adaptive optics in microscopy
US9477074B2 (en) Bessel beam plane illumination microscope
US9581798B2 (en) Light sheet-based imaging device with extended depth of field
Quirin et al. Instantaneous three-dimensional sensing using spatial light modulator illumination with extended depth of field imaging
US11762180B2 (en) Three-photon light sheet imaging
JP6512667B2 (ja) 側方照明顕微鏡システム及び顕微方法
US10401605B2 (en) Structured illumination in inverted light sheet microscopy
CN112005100A (zh) 用于荧光显微镜的光盘显微术
NL2008873C2 (en) Method and apparatus for multiple points of view three-dimensional microscopy.
Landry et al. High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution
US20200049968A1 (en) Flexible light sheet generation by field synthesis
Hsu et al. Metasurface-based Airy light-sheet fluorescence microscopy
Patton et al. Adaptive optics from microscopy to nanoscopy
Power et al. Illumination in Light Sheet Fluorescence Microscopy
Landry High-Speed Isotropic Resolution and Structured Illumination Using Phased Arrays In Light Sheet Fluorescence Microscopy
Vettenburg Planar light-sheet microscopy with curved Airy beams
Lachetta Development and cost-effective implementation of a structured illumination microscope and an optical projection tomography setup
Fiolka Rapid volumetric imaging with light-sheet microscopy
Botcherby et al. Aberration free refocusing for high numerical aperture microscopy
Özgürün A simple and low-cost structured illumination microscopy using a pico-projector
Thomas Adaptive Optics and structured illumination in fluorescence microscopy

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant