CN104204228A - 使用组合的核酸酶、连接和聚合酶反应用于核酸序列的相对定量、表达或拷贝变化的方法 - Google Patents
使用组合的核酸酶、连接和聚合酶反应用于核酸序列的相对定量、表达或拷贝变化的方法 Download PDFInfo
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- CN104204228A CN104204228A CN201380018363.1A CN201380018363A CN104204228A CN 104204228 A CN104204228 A CN 104204228A CN 201380018363 A CN201380018363 A CN 201380018363A CN 104204228 A CN104204228 A CN 104204228A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/501—Ligase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
- C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
- C12Q2533/107—Probe or oligonucleotide ligation
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261598343P | 2012-02-14 | 2012-02-14 | |
| US61/598,343 | 2012-02-14 | ||
| US201261605057P | 2012-02-29 | 2012-02-29 | |
| US61/605,057 | 2012-02-29 | ||
| US201261644405P | 2012-05-08 | 2012-05-08 | |
| US61/644,405 | 2012-05-08 | ||
| PCT/US2013/026180 WO2013123220A1 (en) | 2012-02-14 | 2013-02-14 | Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104204228A true CN104204228A (zh) | 2014-12-10 |
Family
ID=48984705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380018363.1A Pending CN104204228A (zh) | 2012-02-14 | 2013-02-14 | 使用组合的核酸酶、连接和聚合酶反应用于核酸序列的相对定量、表达或拷贝变化的方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US9598728B2 (https=) |
| EP (1) | EP2814986B1 (https=) |
| JP (2) | JP2015508655A (https=) |
| CN (1) | CN104204228A (https=) |
| AU (2) | AU2013221480B2 (https=) |
| CA (1) | CA2863257C (https=) |
| ES (1) | ES2655509T3 (https=) |
| IL (1) | IL233908A0 (https=) |
| SG (1) | SG11201404899VA (https=) |
| WO (1) | WO2013123220A1 (https=) |
Cited By (8)
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|---|---|---|---|---|
| CN107532207A (zh) * | 2015-04-10 | 2018-01-02 | 空间转录公司 | 生物样本的空间区别、多重核酸分析 |
| CN108456716A (zh) * | 2017-02-19 | 2018-08-28 | 南京科维思生物科技股份有限公司 | 用于检测靶基因突变体变异的方法、组合物和试剂盒 |
| CN108795930A (zh) * | 2018-07-09 | 2018-11-13 | 博奥生物集团有限公司 | 一种用于低丰度dna突变的富集技术及其应用 |
| CN109337887A (zh) * | 2018-10-24 | 2019-02-15 | 中国医药集团总公司四川抗菌素工业研究所 | 一种Nucyep酶的编码基因、重组表达载体、重组工程菌及其制备方法和应用 |
| CN114645081A (zh) * | 2020-12-17 | 2022-06-21 | 厦门大学 | 一种检测基因元件的方法和试剂盒 |
| CN114672543A (zh) * | 2022-04-06 | 2022-06-28 | 中国药科大学 | 一种高特异核酸多重可视化检测的方法 |
| CN115312121A (zh) * | 2022-09-29 | 2022-11-08 | 北京齐碳科技有限公司 | 靶基因位点检测方法、装置、介质及程序产品 |
| TWI906439B (zh) * | 2020-12-25 | 2025-12-01 | 日商Ｔｌ基因體科技股份有限公司 | 每個單細胞的特定核酸之拷貝數之檢測方法 |
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| SG11201404899VA (en) | 2014-10-30 |
| JP2017184740A (ja) | 2017-10-12 |
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| US20170212103A1 (en) | 2017-07-27 |
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| AU2018267633A1 (en) | 2018-12-13 |
| CA2863257A1 (en) | 2013-08-22 |
| US20220178916A1 (en) | 2022-06-09 |
| US20190346430A1 (en) | 2019-11-14 |
| EP2814986A4 (en) | 2015-12-09 |
| JP2015508655A (ja) | 2015-03-23 |
| JP6479083B2 (ja) | 2019-03-06 |
| CA2863257C (en) | 2021-12-14 |
| US11209424B2 (en) | 2021-12-28 |
| AU2013221480B2 (en) | 2018-08-23 |
| AU2013221480A1 (en) | 2014-08-21 |
| EP2814986A1 (en) | 2014-12-24 |
| US20150038336A1 (en) | 2015-02-05 |
| EP2814986B1 (en) | 2017-10-25 |
| US20250093341A1 (en) | 2025-03-20 |
| US9598728B2 (en) | 2017-03-21 |
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