CN104189101B - The fat-soluble position of Herba Chelidonii seed and blood fat reducing purposes thereof - Google Patents
The fat-soluble position of Herba Chelidonii seed and blood fat reducing purposes thereof Download PDFInfo
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- CN104189101B CN104189101B CN201410373965.5A CN201410373965A CN104189101B CN 104189101 B CN104189101 B CN 104189101B CN 201410373965 A CN201410373965 A CN 201410373965A CN 104189101 B CN104189101 B CN 104189101B
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Abstract
The present invention relates to a kind of fat-soluble position of Herba Chelidonii seed and blood fat reducing purposes thereof.This fat-soluble position of Herba Chelidonii seed is with Herba Chelidonii mature seed as raw material, uses one or more in petroleum ether, ether, vegetable oil extraction solvent to extract, or uses supercritical fluid extraction to obtain；This fat-soluble position, it is possible to the serum total cholesterol that regulation high fat diet causes, total triglyceride, low-density lipoprotein cholesterol, the ANOMALOUS VARIATIONS of HDL-C, can be used for preparing blood fat reducing food, health food, medicine.
Description
Technical field
The present invention relates to the field of Chinese medicines, specifically, relate to a kind of fat-soluble position of Herba Chelidonii seed and blood fat reducing purposes thereof.
Background technology
In the blood of people, lipid contained in blood plasma is referred to as blood fat, including cholesterol, cholesterol ester, triglyceride, phospholipid and the most esterified fatty acid etc. several, the cholesterol being primarily referred to as in serum and triglyceride.Cholesterol and triglyceride are all the nutrient substance of needed by human, but Healthy People has certain standard level, cholesterol ＜ 5.172mmol/L, triglyceride ＜ 2.032mmol/L.When the average such as cholesterol, triglyceride exceedes normal value, then it is referred to as hyperlipemia.Blood lipid level is too high, can directly cause the disease of some serious harm healths, such as atherosclerosis, coronary heart disease, pancreatitis etc..
Hyperlipemia is commonly encountered diseases and the frequently-occurring disease of person in middle and old age.Along with raising and the change of living habit of people's living standard, the sickness rate of this disease substantially increases, and the age of morbidity has shifted to an earlier date.Atherosclerosis caused by hyperlipemia is to cause the main cause of coronary heart disease, hypertension and cerebrovascular disease, and the whole world there are about 12,000,000 people every year and dies from cardiovascular diseases and apoplexy.So, the treatment of hyperlipemia is extremely important, and this has caused the highest attention of the whole mankind.The final purpose for the treatment of hyperlipemia is the further development of suppression arteriosclerosis, thus prevents the death caused by cardio-cerebrovascular diseases.
Herba ChelidoniiChelidonium majusFor a kind under Papaveraceae Chelidonium.Come from herbal for Relief of Famines, belong to whole grass.For perennial herb, main root is coniform, khaki.Stem is upright, high 30～100 centimetres, and multi-branched has white lead, dredges raw white elongated pubescence, and disconnected has yellow milk.Main root is sturdy, cone, khaki or crineous, close raw fibrous root.Stem is upright, and multi-branched has white lead, has white elongated pubescence.Another name: Herba Munroniae henryi, Niu Jinhua, Radix seu caulis Berberidis Gagnepainii (Radix Berberidis Virgetori), eight steps are tight, Gelsemium elegans Benth., Shanxi melon, Realgar grass, mountain Rhizoma Coptidis, vacation Rhizoma Coptidis, little Herba Stylophori Lasiocapi grass, yellow stuff, Rhizoma Picrorhizae, little Rhizoma Coptidis.All herbal medicine has the effects such as analgesia, cough-relieving, diuresis, removing toxic substances.Cure mainly that stomachache, stomachache, enteritis, dysentery, chronic bronchitis, pertussis, cough, jaundice, edema, ascites, scabies skin ulcer be swollen, snake bite and insect sting.
Research currently, with respect to Herba Chelidonii is concentrated mainly on Herba Chelidonii cultivation, herb chemical composition and pharmacologically active aspect.And having no the relevant report about the fat-soluble position of Herba Chelidonii seed, the hypolipidemic activity at Herba Chelidonii seed and fat-soluble position thereof belongs to reported first.
Summary of the invention
It is an object of the invention to provide a kind of Herba Chelidonii seed extract and application thereof.
The purposes of Herba Chelidonii seed extract provided by the present invention is its application in preparing blood fat-reducing product.
Described product is food, health food and medicine.
Herba Chelidonii seed extract of the present invention is prepared by following method:
Take Herba Chelidonii mature seed, pulverize；Extract with one or more in petroleum ether, ether, vegetable oil extraction solvent, or use supercritical fluid extraction；Recycling design, obtains the fat-soluble position of Herba Chelidonii seed.
Detailed description of the invention
For the more detailed description present invention, be given and following prepare example.But the scope of the present invention is not limited thereto.
Embodiment 1: the preparation at the fat-soluble position of Herba Chelidonii seed
Gather time ripe Herba Chelidonii seed, remove impurity, dry, pulverize；Powder with 10 times amount petroleum ether extract 3 times, each 24 hours；Filter, united extraction liquid, recycling design, obtain the fat-soluble position of Herba Chelidonii seed.
Embodiment 2: the preparation at the fat-soluble position of Herba Chelidonii seed
Gather time ripe Herba Chelidonii seed, remove impurity, dry, pulverize；With carbon dioxide as extractant, use isothermal and isobaric method to carry out supercritical extraction, obtain the fat-soluble position of Herba Chelidonii seed.
Embodiment 3: Herba Chelidonii seed fat-soluble position hypolipidemic activity
1 experiment material
Positive control drug: Monas cuspurpureus Went extract.Serum total cholesterol (TC)；Triglyceride (TG)；HDL-C (HDL cho) and low-density lipoprotein cholesterol (LDL cho) assay test kit, built up Bioengineering Research Institute by Nanjing and produce.
KM
SPF level mice 120,18-22g, purchased from Co., Ltd of Changchun institute of biological products, credit number: SCXK(is lucky) 2011-0003, after adapting to environment, point cage monoculture, it is randomly divided into experimental group (the fat-soluble position of Herba Chelidonii seed high, medium and low dosage group, positive controls, model group) and blank group, often group 10.Preventative to 30 days given the test agent time, therapeutic was to 30 days given the test agent time.
High lipid food: 78.8% normal feedstuff, 1% cholesterol, 10% yolk powder and 10% Adeps Sus domestica, 0.2% cholate.
2 test methods
2.1 hyperlipoidemia method preventive administrations
Under experimental situation, 5d observed by mice feed normal feedstuff, carry out random packet, experimental group gives the given the test agent of various dose while giving high lipid food, blank group gives the distilled water that conventional feed gives same volume simultaneously, periodically weigh in, terminate fasting 16 hours in experiment, survey serum TC, TG, HDL-C, LDL-C level.
2.2 hyperlipoidemia method therapeutic
Under experimental situation, 5d observed by mice feed normal feedstuff, after formal experiment starts to use high lipid food feed 10d instead except blank group (feeding normal feedstuff) each test group of animals, starts to be administered.Given the test agent per os gavage, model group and blank group give the distilled water of same volume, and periodically weigh in, and terminate fasting 16 hours in experiment, measure serum TC, TG, HDL-C, LDL-C level.
3 experimental results
3.1 preventive administration
Preventive administration experimental result is shown in Table 1.
Table
1
Mice preventive administration lipid-lowering test each index situation (
Mean
±
SD
,
n=8
)
Compare with model group: *p0.05, * *p0.01, * * *p0.001
By table 1 data it follows that
(1) T-CHOL: through the High fat diet of 30 days, the TC content of model group mice be significantly higher than blank group (p0.01), illustrate successfully to establish hypercholesterolemia mouse model；Herba Chelidonii seed fat-soluble position high dose group (p0.01), middle dosage group (p0.001), low dose group (p0.001) and positive drug group (p0.05) TC content is all substantially less than model group, makes colza in the wrong son fat-soluble position each dosage group clear and positive drug is respectively provided with the effect reducing TC, and the fat-soluble position of Herba Chelidonii seed each dosage group is superior to positive drug group.
(2) triglyceride: feeding through the high lipid foods of 30 days, the TG content of model group mice is apparently higher than blank group, but not statistically significant；The fat-soluble position of Herba Chelidonii seed each dosage group and positive drug group TG content are below model group, but respectively organize equal not statistically significant.
(3) HDL-C: there is certain difference between the HDL-cho content of each dosage group, but all not statistically significants.
(4) low-density lipoprotein cholesterol: through the High fat diet of 30 days, the LDL-cho content of model group mice be significantly higher than Normal group (p0.05), illustrate successfully to establish high low density lipoprotein cholesterol mouse model；The fat-soluble position of Herba Chelidonii seed each dosage group and positive drug group (p0.01) LDL-cho content is all substantially less than model group, makes colza in the wrong son fat-soluble position each dosage group clear and positive drug is respectively provided with the effect reducing LDL-cho, and the fat-soluble position of Herba Chelidonii seed each dosage group is superior to positive drug group.
3.2 therapeutic
Therapeutic experimental result is shown in Table 2.
Table
2
Mice therapeutic lipid-lowering test each index situation (
Mean
±
SD
,
n=8
)
Compare with model group: *p0.05, * *p0.01, * * *p0.001
By table 2 data it follows that
(1) T-CHOL: through the High fat diet of 30 days, the TC content of model group mice be significantly higher than blank group (p0.001), illustrate successfully to establish hypercholesterolemia mouse model；Herba Chelidonii seed fat-soluble position high dose group (p0.001), middle dosage group (p0.01), low dose group (p0.01) and positive drug group (p0.001) TC content is all substantially less than model group, makes colza in the wrong son fat-soluble position each dosage group clear and positive drug is respectively provided with the effect reducing TC.
(2) triglyceride: through 30 days high lipid foods feed, the TG content of model group mice be significantly higher than blank group (p0.001), illustrate successfully to establish high triglyceride mouse model；Herba Chelidonii seed fat-soluble position high dose group (p0.001), middle dosage group (p0.01) and positive drug group (p0.001) TG content is all substantially less than model group, makes colza in the wrong fat-soluble position high dose group, middle dosage group and positive drug clear and is respectively provided with the effect reducing TG.
(3) HDL-C: feed through the high lipid foods of 30 days, the HDL-cho content of model group mice is significantly lower than blank group, but does not have statistical significance；The fat-soluble position of Herba Chelidonii seed each dosage group and positive drug group HDL-cho content are above model group, but respectively organize equal not statistically significant.
(4) low-density lipoprotein cholesterol: through the High fat diet of 30 days, the LDL-cho content of model group mice be significantly higher than Normal group (p0.001), illustrate successfully to establish high low density lipoprotein cholesterol mouse model；The fat-soluble position of Herba Chelidonii seed each dosage group (p0.001) and positive drug group (p0.01) LDL-cho content is all substantially less than model group, makes colza in the wrong son fat-soluble position each dosage group clear and positive drug is respectively provided with the effect reducing LDL-cho, and the fat-soluble position of Herba Chelidonii seed each dosage group is superior to positive drug group.
4 experiment conclusion
Preventative and the therapeutic lipid-lowering test interpretation of result of mice shows: the fat-soluble position of Herba Chelidonii seed (100mg/kg ~ 400mg/kg) can significantly suppress control and the prevention of arterial of the rising of TC, TG and LDL level of mice, beneficially blood fat atherosis；There is the effect of T-CHOL in blood vessel wall of removing at the fat-soluble position of Herba Chelidonii seed, and this is significant for the incidence rate reducing cardiovascular and cerebrovascular disease.Therefore, utilize the exploitation of Herba Chelidonii seed fat-soluble position to have the food of effect for reducing blood fat, health food and medicine and there is good prospect.
Claims (1)
1. Herba Chelidonii seed fat-soluble position application in preparing blood lipid-lowering medicine, it is characterised in that: take Herba Chelidonii mature seed, pulverize；Extract with one or more in petroleum ether, ether, vegetable oil extraction solvent, or use supercritical fluid extraction；Recycling design, obtains the fat-soluble position of Herba Chelidonii seed.
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