CN104171800A - Isomaltooligosacharide-fructooligosaccharide composite particle and preparation method thereof - Google Patents
Isomaltooligosacharide-fructooligosaccharide composite particle and preparation method thereof Download PDFInfo
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- CN104171800A CN104171800A CN201410389991.7A CN201410389991A CN104171800A CN 104171800 A CN104171800 A CN 104171800A CN 201410389991 A CN201410389991 A CN 201410389991A CN 104171800 A CN104171800 A CN 104171800A
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- fos
- oligoisomaltose
- liquid glucose
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- 238000002360 preparation method Methods 0.000 title claims abstract description 44
- 239000011246 composite particle Substances 0.000 title claims abstract description 35
- 229940107187 fructooligosaccharide Drugs 0.000 title abstract 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 86
- 229920002472 Starch Polymers 0.000 claims description 46
- 235000019698 starch Nutrition 0.000 claims description 46
- 239000008107 starch Substances 0.000 claims description 46
- 102000004190 Enzymes Human genes 0.000 claims description 43
- 108090000790 Enzymes Proteins 0.000 claims description 43
- 229940088598 enzyme Drugs 0.000 claims description 43
- 108090000637 alpha-Amylases Proteins 0.000 claims description 36
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 35
- 239000000463 material Substances 0.000 claims description 34
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 27
- 229930006000 Sucrose Natural products 0.000 claims description 26
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 26
- 239000005720 sucrose Substances 0.000 claims description 26
- 238000010612 desalination reaction Methods 0.000 claims description 25
- 229930182470 glycoside Natural products 0.000 claims description 23
- 150000002338 glycosides Chemical class 0.000 claims description 23
- 239000007788 liquid Substances 0.000 claims description 23
- 238000006243 chemical reaction Methods 0.000 claims description 22
- 229910052799 carbon Inorganic materials 0.000 claims description 21
- ZCLAHGAZPPEVDX-UHFFFAOYSA-N D-panose Natural products OC1C(O)C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC1COC1C(O)C(O)C(O)C(CO)O1 ZCLAHGAZPPEVDX-UHFFFAOYSA-N 0.000 claims description 20
- AYRXSINWFIIFAE-SCLMCMATSA-N Isomaltose Natural products OC[C@H]1O[C@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)[C@@H](O)[C@@H](O)[C@@H]1O AYRXSINWFIIFAE-SCLMCMATSA-N 0.000 claims description 20
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 claims description 20
- ZCLAHGAZPPEVDX-MQHGYYCBSA-N panose Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@@H](O[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)C=O)O[C@@H]1CO[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 ZCLAHGAZPPEVDX-MQHGYYCBSA-N 0.000 claims description 20
- 238000007445 Chromatographic isolation Methods 0.000 claims description 19
- DBTMGCOVALSLOR-AXAHEAMVSA-N galactotriose Natural products OC[C@@H]1O[C@@H](O[C@@H]2[C@@H](O)[C@H](CO)O[C@@H](O[C@H]3[C@@H](O)[C@H](O)O[C@@H](CO)[C@@H]3O)[C@@H]2O)[C@H](O)[C@H](O)[C@H]1O DBTMGCOVALSLOR-AXAHEAMVSA-N 0.000 claims description 19
- FBJQEBRMDXPWNX-FYHZSNTMSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H]2[C@H]([C@H](O)[C@@H](O)C(O)O2)O)O1 FBJQEBRMDXPWNX-FYHZSNTMSA-N 0.000 claims description 19
- 239000000843 powder Substances 0.000 claims description 19
- 102000004139 alpha-Amylases Human genes 0.000 claims description 18
- 229940024171 alpha-amylase Drugs 0.000 claims description 18
- 108010019077 beta-Amylase Proteins 0.000 claims description 18
- 241000233866 Fungi Species 0.000 claims description 17
- 241000228245 Aspergillus niger Species 0.000 claims description 14
- 238000002834 transmittance Methods 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 230000001105 regulatory effect Effects 0.000 claims description 12
- 238000005469 granulation Methods 0.000 claims description 10
- 230000003179 granulation Effects 0.000 claims description 10
- 238000004061 bleaching Methods 0.000 claims description 9
- 239000003480 eluent Substances 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 9
- 239000008187 granular material Substances 0.000 claims description 9
- 238000005342 ion exchange Methods 0.000 claims description 9
- 239000002002 slurry Substances 0.000 claims description 9
- 239000004382 Amylase Substances 0.000 claims description 8
- 208000007976 Ketosis Diseases 0.000 claims description 7
- FLDFNEBHEXLZRX-DLQNOBSRSA-N Nystose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(O[C@@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 FLDFNEBHEXLZRX-DLQNOBSRSA-N 0.000 claims description 7
- 230000002411 adverse Effects 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 238000004255 ion exchange chromatography Methods 0.000 claims description 7
- 150000002584 ketoses Chemical class 0.000 claims description 7
- FLDFNEBHEXLZRX-UHFFFAOYSA-N nystose Natural products OC1C(O)C(CO)OC1(CO)OCC1(OCC2(OC3C(C(O)C(O)C(CO)O3)O)C(C(O)C(CO)O2)O)C(O)C(O)C(CO)O1 FLDFNEBHEXLZRX-UHFFFAOYSA-N 0.000 claims description 7
- 230000035484 reaction time Effects 0.000 claims description 6
- MYPRUMQOPMOOSZ-UHFFFAOYSA-N 1-(diaminomethylidene)-3-(3-sulfamoylphenyl)urea;hydrochloride Chemical compound Cl.NC(N)=NC(=O)NC1=CC=CC(S(N)(=O)=O)=C1 MYPRUMQOPMOOSZ-UHFFFAOYSA-N 0.000 claims description 2
- 101100174180 Caenorhabditis elegans fos-1 gene Proteins 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 6
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 2
- 239000002245 particle Substances 0.000 abstract description 2
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 abstract 2
- 239000006041 probiotic Substances 0.000 abstract 1
- 235000018291 probiotics Nutrition 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 25
- 238000012360 testing method Methods 0.000 description 9
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 7
- 244000005700 microbiome Species 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 6
- 238000002347 injection Methods 0.000 description 5
- 239000007924 injection Substances 0.000 description 5
- 230000000968 intestinal effect Effects 0.000 description 5
- 238000002955 isolation Methods 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 102000004357 Transferases Human genes 0.000 description 4
- 108090000992 Transferases Proteins 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 3
- 229920000945 Amylopectin Polymers 0.000 description 2
- 229920000856 Amylose Polymers 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 241000186000 Bifidobacterium Species 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- -1 feed Substances 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012543 microbiological analysis Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/22—Preparation of compounds containing saccharide radicals produced by the action of a beta-amylase, e.g. maltose
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01002—Beta-amylase (3.2.1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01041—Pullulanase (3.2.1.41)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention relates to an iosomaltooligosacharide-fructooligosaccharide composite particle and a preparation method thereof. The iosomaltooligosacharide-fructooligosaccharide composite particle comprises the following components in percentage by weight: 51-99% of iosomaltooligosacharide and 1-49% of fructooligosaccharide. The invention also relates to the preparation method of the composite particle. By virtue of the preparation method of the composite particle, iosomaltooligosacharide is firstly mixed with fructooligosaccharide according to a ratio for preparing the particle; the prepared composite particle has the advantages of good fluidity, high dissolubility, no moisture absorption, long shelf life, good effect of increasing probiotics in the intestinal tracts of the human bodies and the like, and is low in production cost; the additional value of iosomaltooligosacharide is greatly enhanced.
Description
Technical field
The present invention relates to a kind of oligoisomaltose and FOS composite particles and preparation method thereof, belong to functional sugar preparing technical field.
Technical background
Oligoisomaltose (Isomaltooligosacharide) claim again bifurcation oligose, refer to that glucosyl group is with α-1, the monose number that 6 glycosidic bonds are combined into, at 2～6 class compound sugar that do not wait, because molecular conformation is different, is called oligoisomaltose for being different from maltose.
Oligoisomaltose has good physicochemical property and physiological function, as low in sugariness, is 40%～50% of sucrose, and the soft U.S. of sweet taste, and mouthfeel is more refreshing; Be difficult to be digested by gastric enzyme, heat is low, does not substantially increase blood pressure and blood lipoid; Promote the propagation of Bifidobacterium in human body intestinal canal, can suppress the formation of the interior harmful bacteria of enteron aisle and corrupt substance.In addition, oligomeric different wheat sugar has the performance of anti-carious tooth etc.In view of characteristic and the health care of oligoisomaltose, its range of application is very extensive, can be applied to the industries such as food, medicine, feed, cosmetics.
The research and development of China's functional oligose start from the beginning of the nineties, what form at present suitability for industrialized production mainly contains oligoisomaltose, FOS, xylo-oligosaccharide, wherein the most cheap with oligoisomaltose price, occupation rate of market is the highest, the problem of puzzlement oligoisomaltose industry development mainly contains two at present: the one, and the hygroscopicity problem of oligoisomaltose itself, easily form caking, the application to it in downstream product has a negative impact; The 2nd, oligoisomaltose to the value-added effect of beneficial bacteria of intestinal tract compared with FOS and xylo-oligosaccharide a little less than, downstream product client more and more tends to use FOS and xylo-oligosaccharide and does not use oligoisomaltose.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, a kind of oligoisomaltose and FOS composite particles and preparation method thereof are provided, the oligoisomaltose of preparing by the present invention and FOS composite particles have good fluidity, soluble, nonhygroscopic, shelf life of products long, to advantages such as human body intestinal canal probio value-added effect are good, effectively solved two problems of above puzzlement oligoisomaltose industry development.
In order to realize object of the present invention, the present invention adopts following technical scheme:
A composite particles for oligoisomaltose and FOS, component is as follows, is all weight percentage:
Oligoisomaltose 51%～99%, FOS 1%～49%.
Preferred according to the present invention, component is as follows, is all weight percentage:
Oligoisomaltose 60%～80%, FOS 20%～40%.
The preparation method of the composite particles of above-mentioned oligoisomaltose and FOS, step is as follows:
(1) by oligoisomaltose liquid glucose through concentrated, make mass concentration and be 60～70% oligoisomaltose liquid,
(2) FOS liquid glucose warp is concentrated, dry, make FOS powder,
(3) the FOS powder that oligoisomaltose liquid step (1) being made and step (2) make adds in fluid bed granulator granulation in proportion, under 50～80 ℃ of temperature conditions, granulate, cross after 40～60 order vibratory sieves, make the composite particles of oligoisomaltose and FOS;
Or step is as follows:
(a) oligoisomaltose liquid glucose warp is concentrated, dry, make oligoisomaltose powder,
(b) by FOS liquid glucose through concentrated, make mass concentration and be 40～50% FOS liquid,
(c) the FOS liquid that oligoisomaltose powder step (a) being made and step (b) make adds in fluid bed granulator granulation in proportion; under 50～80 ℃ of temperature conditions, granulate; cross after 40～60 order vibratory sieves, make the composite particles of oligoisomaltose and FOS.
Preferred according to the present invention, described step (1) or (a) in the preparation method of oligoisomaltose liquid glucose as follows:
(I) regulating starch slurry concentration, after adjusting pH, add high temperature resistant AMS, is 20%～25% liquefier through liquefying, making DE value;
(II) in the liquefier making to step (I), add beta amylase, Pullulanase, fungi alpha amylase, carry out after saccharification react, then add phlorose to turn glycosides enzyme, proceed to turn glycosides reaction, the enzyme that then goes out is lived, and makes liquid glucose;
(III) in the liquid glucose making to step (II), add active carbon, after decolouring, with adverse current ion-exchange chromatography, carry out ion-exchange desalination, make desalination liquid glucose;
(IV) desalination liquid glucose step (III) being made is through chromatographic isolation, the eluent that collection contains isomaltose, panose and Isomaltotriose, makes the oligoisomaltose liquid glucose that isomaltose+panose in total reducing sugar+Isomaltotriose content is not less than 95wt%.
Further preferred according to the present invention, in described step (I), starch slurry concentration is 15～20 Baume degrees, and pH value is 4～6.5, and the addition of high-temperatureα-amylase is that starch material per ton adds 1.2 * 10
7～2.0 * 10
7u, condensing temperature is 110～141 ℃.More excellent, described liquefaction is steam ejection liquefaction, liquefaction number of times is twice.
Further preferred according to the present invention, in described step (II), the addition of described beta amylase is that starch material per ton adds 1.0 * 10
8～1.5 * 10
8the addition of U, Pullulanase is that starch material per ton adds 1.2 * 10
5～1.5 * 10
5the addition of ASPU, fungi alpha amylase is that starch material per ton adds 0.1 * 10
8～0.3 * 10
8u is saccharification react 2～5h under the condition of 50～60 ℃ in temperature; The addition that described phlorose turns glycosides enzyme is that starch material per ton adds 1.8 * 10
8～3.6 * 10
8u is under the condition of 55～60 ℃, to turn glycosides reaction 26～32h in temperature.
Further preferred according to the present invention, in described step (III), the addition of active carbon is 0.3～0.5wt%, bleaching time 1.5～2.0h; Light transmittance >=85% of described desalination liquid glucose after ion-exchange desalination.
Further preferred according to the present invention, in described step (IV), chromatographic isolation service condition is operating pressure 0.25～0.35MPa, 65～70 ℃ of temperature, water loss-rate 1:1.2～1:1.5, charging 1.5～1.7m per hour
3, collect the eluent that contains isomaltose, panose and Isomaltotriose, make the oligoisomaltose liquid glucose that isomaltose+panose in total reducing sugar+Isomaltotriose content is not less than 95wt%.
Preferred according to the present invention, described step (2) or (b) in the preparation method of FOS liquid glucose as follows:
(i) sucrose solution that preparation mass concentration is 25%～60%;
(ii) in the sucrose solution making to step (i), add β-transfructosylase, after reaction, make the thick solution of FOS;
(iii) step (ii) is made the thick solution of FOS through decolouring, filter, from handing over, chromatographic isolation, make the FOS liquid glucose that FOS content in total reducing sugar is not less than 95wt%.
Further preferred according to the present invention, in described step (ii), β-transfructosylase originates from Aspergillus niger strain BLCY-02, and the addition of this enzyme is that sucrose per ton adds 0.5 * 10
6～1.5 * 10
6u, 20～40 ℃ of reaction temperatures, the reaction time is 20～36h.It is CGMCC No.9449 that Aspergillus niger strain BLCY-02 is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) preserving number, β-transfructosylase that this bacterial strain produces is ectoenzyme, after isolation technics separation, separated enzyme liquid enzyme work can reach 1000U/ml routinely.
Further preferred according to the present invention, in described step (iii), decolour for activated carbon decolorizing, active carbon addition 0.1～0.3wt%, bleaching time 1～1.5h; Be filtered into plate-frame filtering; Through solution light transmittance >=85% after friendship.
Further preferred according to the present invention, in described step (iii), chromatographic isolation service condition is operating pressure 0.2～0.35MPa, 60～70 ℃ of temperature, water loss-rate 1:(1.2～1.5), charging 1.8～2.0m per hour
3, collect ketose, Nystose, GF4;
The high temperature resistant AMS that the present invention adopts act as and makes starch liquefaction and generate low molecule rapidly, so those skilled in the art can select corresponding high temperature resistant AMS according to actual needs, the high temperature resistant AMS of selling as Genencor Company.
The beta amylase that the present invention adopts act as from non reducing end successively take maltose as unit cut-out α-1,4-dextran chain, therefore those skilled in the art can select corresponding beta amylase according to actual needs, as the beta amylase of Shandong Anke Bioengineering Co., Ltd.'s sale.
The fungi alpha amylase that the present invention adopts is endo-amylase, can be hydrolyzed rapidly α-1 of gelatinized starch, amylose and amylopectin aqueous solution inside, 4 glucoside bonds, produce soluble dextrins and minority maltose and glucose, therefore those skilled in the art can select corresponding fungi alpha amylase according to actual needs, the fungi alpha amylase that as grand in Shandong mcroorganism Engineering Co., Ltd sells.
The Pullulanase that the present invention adopts act as selectivity and cuts α-1 in amylopectin branch point, 6 glycosidic bonds, cut whole branched structure, form amylose, therefore those skilled in the art can select corresponding Pullulanase according to actual needs, as the Pullulanase of Genencor Company's sale.
The alpha-glucosaccharase transferase that the present invention adopts act as α-1 of interrupting maltose, 4 glycosidic bonds, and by a free glucose residue with α-1, the form of 6 glycosidic bonds is connected on another glucose or maltose molecule, generate the non-fermentable carbohydrates such as isomaltose and panose, therefore those skilled in the art can select corresponding alpha-glucosaccharase transferase according to actual needs, the alpha-glucosaccharase transferase of selling as Japanese Tian Ye company.
Beneficial effect
The present invention is first in proportion by oligomeric maltose and FOS mixing granulation, prepared composite particles have good fluidity, soluble, nonhygroscopic, shelf life of products long, to advantages such as human body intestinal canal probio value-added effect are good, and production cost is low, greatly improved the added value of oligomeric maltose.
The specific embodiment:
Below in conjunction with embodiment, technical scheme of the present invention is further elaborated, but institute of the present invention protection domain is not limited to this.
Raw material sources
Aspergillus niger (Aspergillus niger) BLCY-02, within on 07 15th, 2014, be stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number CGMCC No.9449, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica.
High temperature resistant AMS, Genencor Company is on sale, and enzyme is lived as 20000U/mL;
Beta amylase, Shandong Anke Bioengineering Co., Ltd. is on sale; Enzyme is lived as 100000U/g;
Fungal alpha-amylase, Shandong grand mcroorganism Engineering Co., Ltd is on sale; Enzyme is lived as 100000U/g;
Pullulanase, Genencor Company is on sale; Enzyme is lived as 1000ASPU/ml;
Alpha-glucosaccharase transferase, Japanese Tian Ye company is on sale; Enzyme is lived as 300000U/mL;
Embodiment 1
The preparation method of the composite particles of oligoisomaltose and FOS, step is as follows:
(1) by oligoisomaltose liquid glucose through concentrated, make mass concentration and be 60% oligoisomaltose liquid,
(2) FOS liquid glucose warp is concentrated, dry, make FOS powder,
(3) the FOS powder that oligoisomaltose liquid step (1) being made and step (2) make adds in fluid bed granulator granulation; under 50 ℃ of temperature conditions, granulate; cross after 40 order vibratory sieves, make the composite particles of oligoisomaltose and FOS.
The preparation method of described oligoisomaltose liquid glucose is as follows:
(I) regulating starch slurry concentration is 15 Baume degrees, after regulating pH to be 4, adds high temperature resistant AMS, and the addition of high-temperatureα-amylase is 0.6L/ ton dried starch, at 125 ℃, through secondary injection liquefaction, makes DE value and be 20% liquefier;
(II) in the liquefier making to step (I), add beta amylase, Pullulanase, fungi alpha amylase, the addition of beta amylase is that addition that starch material per ton adds 1.3kg, Pullulanase is that the addition that starch material per ton adds 0.12L, fungi alpha amylase is that starch material per ton adds 0.25kg, at 55 ℃, carry out saccharification react 5h, add again phlorose to turn glycosides enzyme, the addition that phlorose turns glycosides enzyme is that starch material per ton adds 0.8L, at 55 ℃, proceed to turn glycosides reaction 26h, at the 70 ℃ of enzymes that go out 5min alive, make liquid glucose;
(III) in the liquid glucose making to step (II), add active carbon, the addition of active carbon is 0.4wt%, after decolouring 2h, with adverse current ion-exchange chromatography, carries out ion-exchange desalination, makes light transmittance and be 90% desalination liquid glucose;
(IV) desalination liquid glucose step (III) being made is through chromatographic isolation, and chromatographic isolation service condition is operating pressure 0.25,65 ℃ of temperature, water loss-rate 1:1.3, charging 1.5m per hour
3, collect the eluent that contains isomaltose, panose and Isomaltotriose, make the oligoisomaltose liquid glucose of isomaltose+panose in total reducing sugar+Isomaltotriose content 95wt%.
The preparation method of described FOS liquid glucose is as follows:
(i) sucrose solution that preparation mass concentration is 45%;
(ii) in the sucrose solution making to step (i), add β-transfructosylase, β-transfructosylase addition is 0.5L/ ton sucrose, and reaction 20h, makes the thick solution of FOS;
(iii) step (ii) is made to the thick solution of FOS through activated carbon decolorizing, active carbon addition is 0.1wt%, bleaching time is 1.5h, then through plate-frame filtering, then through from friendship, obtain light transmittance and be 85% liquid glucose, through operating pressure 0.2MPa, temperature 60 C, water loss-rate 1:1.2, charging 1.8m per hour
3, collect ketose, Nystose, GF4, make the FOS liquid glucose that FOS content in total reducing sugar is not less than 95wt%.
In described step (ii), β-transfructosylase originates from Aspergillus niger strain BLCY-02, and the addition of this enzyme is that sucrose per ton adds 0.5 * 10
6u, 20 ℃ of reaction temperatures, the reaction time is 36h.It is CGMCC No.9449 that Aspergillus niger strain BLCY-02 is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) preserving number, β-transfructosylase that this bacterial strain produces is ectoenzyme, after isolation technics separation, separated enzyme liquid enzyme work can reach 1000U/ml routinely.
After testing, in the composite particles of oligoisomaltose and FOS, the mass percent of oligoisomaltose is 51%, and the mass percent of FOS is 49%.
Embodiment 2
The preparation method of the composite particles of oligoisomaltose and FOS, step is as follows:
(a) by oligoisomaltose liquid glucose, through being concentrated into mass concentration, be 70%, then dry, make oligoisomaltose powder,
(b) by FOS liquid glucose through concentrated, make mass concentration and be 50% FOS liquid,
(c) the FOS liquid that oligoisomaltose powder step (a) being made and step (b) make adds in fluid bed granulator granulation in proportion; under 60 temperature conditions, granulate; cross after 60 order vibratory sieves, make the composite particles of oligoisomaltose and FOS.
The preparation method of described oligoisomaltose liquid glucose is as follows:
(I) regulating starch slurry concentration is 17 Baume degrees, after regulating pH to be 6.5, adds high temperature resistant AMS, and the addition of high-temperatureα-amylase is that starch material per ton adds 1.0L, at 130 ℃, through secondary injection liquefaction, makes DE value and be 25% liquefier;
(II) in the liquefier making to step (I), add beta amylase, Pullulanase, fungi alpha amylase, the addition of beta amylase is that addition that starch material per ton adds 1.25kg, Pullulanase is that the addition that starch material per ton adds 0.13L, fungi alpha amylase is that starch material per ton adds 0.15kg, at 55 ℃, carry out saccharification react 5h, add again phlorose to turn glycosides enzyme, the addition that phlorose turns glycosides enzyme is that starch material per ton adds 1.2L, at 55 ℃, proceed to turn glycosides reaction 32h, at the 80 ℃ of enzymes that go out 5min alive, make liquid glucose;
(III) in the liquid glucose making to step (II), add active carbon, the addition of active carbon is 0.4wt%, after decolouring 1.7h, with adverse current ion-exchange chromatography, carries out ion-exchange desalination, makes light transmittance and be 90% desalination liquid glucose;
(IV) desalination liquid glucose step (III) being made is through chromatographic isolation, and chromatographic isolation service condition is operating pressure 0.3MPa, 68 ℃ of temperature, water loss-rate 1:1.4, charging 1.7m per hour
3, collect the eluent that contains isomaltose, panose and Isomaltotriose, make the oligoisomaltose liquid glucose of isomaltose+panose in total reducing sugar+Isomaltotriose content 96wt%.
The preparation method of described FOS liquid glucose is as follows:
(i) sucrose solution that preparation mass concentration is 60%%;
(ii) in the sucrose solution making to step (i), add β-transfructosylase, β-transfructosylase addition is 1.5L/ ton sucrose, and reaction 36h, makes the thick solution of FOS;
(iii) step (ii) is made to the thick solution of FOS through activated carbon decolorizing, active carbon addition is 0.2wt%, bleaching time is 1.5h, then through plate-frame filtering, then through from friendship, obtain light transmittance and be 87% liquid glucose, through operating pressure 0.35MPa, temperature 70 C, water loss-rate 1:1.5, charging 2.0m per hour
3, collect under ketose, Nystose, GF4 condition and carry out chromatographic isolation, make the FOS liquid glucose of FOS content 97wt% in total reducing sugar.
In described step (ii), β-transfructosylase can be with reference to < < Preparation of fructosyltransferasefrom from Aspergillus niger VVTP 84 > > (Zheng Shi Jin Yun etc., the 22nd the 1st phase of volume of the journal > > of < < Wuxi Light Industry Univ., in January, 2003) the record preparation in a literary composition.
After testing, in the composite particles of oligoisomaltose and FOS, the mass percent of oligoisomaltose is 99%, and the mass percent of FOS is 1%.
Embodiment 3
The preparation method of the composite particles of oligoisomaltose and FOS, step is as follows:
(a) by oligoisomaltose liquid glucose, through being concentrated into mass concentration, be 65%, then dry, make oligoisomaltose powder,
(b) by FOS liquid glucose through concentrated, make mass concentration and be 45% FOS liquid,
(c) the FOS liquid that oligoisomaltose powder step (a) being made and step (b) make adds in fluid bed granulator granulation in proportion; under 80 ℃ of temperature conditions, granulate; cross after 50 order vibratory sieves, make the composite particles of oligoisomaltose and FOS.
The preparation method of described oligoisomaltose liquid glucose is as follows:
(I) regulating starch slurry concentration is 20 Baume degrees, after regulating pH to be 5.5, adds high temperature resistant AMS, and the addition of high-temperatureα-amylase is that starch material per ton adds 0.8L, at 141 ℃, through secondary injection liquefaction, makes DE value and be 22% liquefier;
(II) in the liquefier making to step (I), add beta amylase, Pullulanase, fungi alpha amylase, the addition of beta amylase is that addition that starch material per ton adds 1.5kg, Pullulanase is that the addition that starch material per ton adds 0.13L, fungi alpha amylase is that starch material per ton adds 0.3kg, at 60 ℃, carry out saccharification react 4h, add again phlorose to turn glycosides enzyme, the addition that phlorose turns glycosides enzyme is that starch material per ton adds 0.8L, at 58 ℃, proceed to turn glycosides reaction 28h, at the 75 ℃ of enzymes that go out 10min alive, make liquid glucose;
(III) in the liquid glucose making to step (II), add active carbon, the addition of active carbon is 0.5wt%, after decolouring 2h, with adverse current ion-exchange chromatography, carries out ion-exchange desalination, makes light transmittance and be 90% desalination liquid glucose;
(IV) desalination liquid glucose step (III) being made is through chromatographic isolation, and chromatographic isolation service condition is operating pressure 0.35MPa, temperature 70 C, water loss-rate 1:1.4, charging 1.65m per hour
3, collect the eluent that contains isomaltose, panose and Isomaltotriose, make the oligoisomaltose liquid glucose of isomaltose+panose in total reducing sugar+Isomaltotriose content 96wt%.
The preparation method of described FOS liquid glucose is as follows:
(i) sucrose solution that preparation mass concentration is 30%%;
(ii) in the sucrose solution making to step (i), add β-transfructosylase, β-transfructosylase addition is 1.25L/ ton sucrose, and reaction 28h, makes the thick solution of FOS;
(iii) step (ii) is made to the thick solution of FOS through activated carbon decolorizing, active carbon addition is 0.3wt%, bleaching time is 1.5h, then through plate-frame filtering, then through from friendship, obtain light transmittance and be 90% liquid glucose, condition is operating pressure 0.35MPa, 65 ℃ of temperature, water loss-rate 1:1.45, charging 1.9m per hour
3, collection ketose, Nystose, GF4 make the FOS liquid glucose of FOS content 96wt% in total reducing sugar.
In described step (ii), β-transfructosylase originates from Aspergillus niger strain BLCY-02, and the addition of this enzyme is that sucrose per ton adds 1.5 * 10
6u, 40 ℃ of reaction temperatures, the reaction time is 20h.It is CGMCC No.9449 that Aspergillus niger strain BLCY-02 is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) preserving number, β-transfructosylase that this bacterial strain produces is ectoenzyme, after isolation technics separation, separated enzyme liquid enzyme work can reach 1000U/ml routinely.
After testing, in the composite particles of oligoisomaltose and FOS, the mass percent of oligoisomaltose is 75%, and the mass percent of FOS is 25%.
Embodiment 4
The preparation method of the composite particles of oligoisomaltose and FOS, step is as follows:
(1) by oligoisomaltose liquid glucose through concentrated, make mass concentration and be 70% oligoisomaltose liquid,
(2) FOS liquid glucose warp is concentrated, dry, make FOS powder,
(3) the FOS powder that oligoisomaltose liquid step (1) being made and step (2) make adds in fluid bed granulator granulation; under 60 ℃ of temperature conditions, granulate; cross after 40 order vibratory sieves, make the composite particles of oligoisomaltose and FOS.
The preparation method of described oligoisomaltose liquid glucose is as follows:
(I) regulating starch slurry concentration is 17 Baume degrees, after regulating pH to be 5, adds high temperature resistant AMS, and the addition of high-temperatureα-amylase is that starch material per ton adds 0.8L, at 135 ℃, through secondary injection liquefaction, makes DE value and be 22% liquefier;
(II) in the liquefier making to step (I), add beta amylase, Pullulanase, fungi alpha amylase, the addition of beta amylase is that addition that starch material per ton adds 0.1L, Pullulanase is that the addition that starch material per ton adds 0.12L, fungi alpha amylase is that starch material per ton adds 0.1L, at 50 ℃, carry out saccharification react 5h, add again phlorose to turn glycosides enzyme, the addition that phlorose turns glycosides enzyme is that starch material per ton adds 0.6L, at 55 ℃, proceed to turn glycosides reaction 26h, at the 70 ℃ of enzymes that go out 5min alive, make liquid glucose;
(III) in the liquid glucose making to step (II), add active carbon, the addition of active carbon is 0.3wt%, after decolouring 2h, with adverse current ion-exchange chromatography, carries out ion-exchange desalination, makes light transmittance and be 85% desalination liquid glucose;
(IV) desalination liquid glucose step (III) being made is through chromatographic isolation, and chromatographic isolation service condition is operating pressure 0.25,65 ℃ of temperature, water loss-rate 1:1.2, charging 1.5m per hour
3, collect the eluent that contains isomaltose, panose and Isomaltotriose, make the oligoisomaltose liquid glucose of isomaltose+panose in total reducing sugar+Isomaltotriose content 95wt%.
The preparation method of described FOS liquid glucose is as follows:
(i) sucrose solution that preparation mass concentration is 25%;
(ii) in the sucrose solution making to step (i), add β-transfructosylase, β-transfructosylase addition is 1L/ ton sucrose, and reaction 20h, makes the thick solution of FOS;
(iii) step (ii) is made to the thick solution of FOS through activated carbon decolorizing, active carbon addition is 0.1wt%, bleaching time is 1.5h, then through plate-frame filtering, then through from friendship, obtain light transmittance and be 85% liquid glucose, through operating pressure 0.2MPa, temperature 60 C, water loss-rate 1:1.3, charging 1.8m per hour
3, collect ketose, Nystose, GF4, make the FOS liquid glucose that FOS content in total reducing sugar is not less than 95wt%.
In described step (ii), β-transfructosylase originates from Aspergillus niger strain BLCY-02, and the addition of this enzyme is that sucrose per ton adds 1.0 * 10
6u, 30 ℃ of reaction temperatures, the reaction time is 30h.It is CGMCC No.9449 that Aspergillus niger strain BLCY-02 is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) preserving number, β-transfructosylase that this bacterial strain produces is ectoenzyme, after isolation technics separation, separated enzyme liquid enzyme work can reach 1000U/ml routinely.
After testing, in the composite particles of oligoisomaltose and FOS, the mass percent of oligoisomaltose is 60%, and the mass percent of FOS is 40%.
Embodiment 5
The preparation method of the composite particles of oligoisomaltose and FOS, step is as follows:
(a) by oligoisomaltose liquid glucose, through being concentrated into mass concentration, be 66%, then dry, make oligoisomaltose powder,
(b) by FOS liquid glucose through concentrated, make mass concentration and be 46% FOS liquid,
(c) the FOS liquid that oligoisomaltose powder step (a) being made and step (b) make adds in fluid bed granulator granulation in proportion; under 75 ℃ of temperature conditions, granulate; cross after 50 order vibratory sieves, make the composite particles of oligoisomaltose and FOS.
The preparation method of described oligoisomaltose liquid glucose is as follows:
(I) regulating starch slurry concentration is 19 Baume degrees, after regulating pH to be 5.5, adds high temperature resistant AMS, and the addition of high-temperatureα-amylase is that starch material per ton adds 0.9L, at 141 ℃, through secondary injection liquefaction, makes DE value and be 25% liquefier;
(II) in the liquefier making to step (I), add beta amylase, Pullulanase, fungi alpha amylase, the addition of beta amylase is that addition that starch material per ton adds 1.4kg, Pullulanase is that the addition that starch material per ton adds 0.13L, fungi alpha amylase is that starch material per ton adds 0.25kg, at 55 ℃, carry out saccharification react 5h, add again phlorose to turn glycosides enzyme, the addition that phlorose turns glycosides enzyme is that starch material per ton adds 1.0L, at 60 ℃, proceed to turn glycosides reaction 30h, at the 75 ℃ of enzymes that go out 10min alive, make liquid glucose;
(III) in the liquid glucose making to step (II), add active carbon, the addition of active carbon is 0.5wt%, after decolouring 2h, with adverse current ion-exchange chromatography, carries out ion-exchange desalination, makes light transmittance and be 90% desalination liquid glucose;
(IV) desalination liquid glucose step (III) being made is through chromatographic isolation, and chromatographic isolation service condition is operating pressure 0.35MPa, temperature 70 C, water loss-rate 1:1.4, charging 1.65m per hour
3, collect the eluent that contains isomaltose, panose and Isomaltotriose, make the oligoisomaltose liquid glucose of isomaltose+panose in total reducing sugar+Isomaltotriose content 95wt%.
The preparation method of described FOS liquid glucose is as follows:
(i) sucrose solution that preparation mass concentration is 50%;
(ii) in the sucrose solution making to step (i), add β-transfructosylase, β-transfructosylase addition is 1.5L/ ton sucrose, and reaction 30h, makes the thick solution of FOS;
(iii) step (ii) is made to the thick solution of FOS through activated carbon decolorizing, active carbon addition is 0.2wt%, bleaching time is 1.0h, then through plate-frame filtering, then through from friendship, obtain the liquid glucose of light transmittance 90%, condition is operating pressure 0.3MPa, 65 ℃ of temperature, water loss-rate 1:1.3, charging 2.0m per hour
3, collection ketose, Nystose, GF4 make the FOS liquid glucose of FOS content 95wt% in total reducing sugar.
In described step (ii), β-transfructosylase originates from Aspergillus niger strain BLCY-02, and the addition of this enzyme is that sucrose per ton adds 0.8 * 10
6u, 25 ℃ of reaction temperatures, the reaction time is 33h.It is CGMCC No.9449 that Aspergillus niger strain BLCY-02 is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) preserving number, β-transfructosylase that this bacterial strain produces is ectoenzyme, after isolation technics separation, separated enzyme liquid enzyme work can reach 1000U/ml routinely.
After testing, in the composite particles of oligoisomaltose and FOS, the mass percent of oligoisomaltose is 80%, and the mass percent of FOS is 20%.
Test example
The product of embodiment 1-5 and oligoisomaltose Icing Sugar (isomaltose+panose in total reducing sugar+Isomaltotriose content reaches 96wt%) are contrasted, product mobility adopts angle of repose index to carry out comparative evaluation, angle of repose refers to that powder or particle packing become the hypotenuse of steepest heap and the angle between horizontal plane, mobility often represents with angle of repose, angle of repose small flow is good, otherwise contrary.
Moisture absorption (hydroscopicity) is calculated as follows:
Hydroscopicity=(sample quality-absolute dried sample quality after moisture absorption)/absolute dried sample quality
After testing, testing result is as shown in table 1:
Table 1
Human body intestinal canal probio value-added effect detects according to the method for GB < < GB4789.35-2010 food security national standard food microbiological analysis lactic acid bacteria check > > regulation and carries out bacterial classification Identification and detection, as shown in table 2 to human body intestinal canal probio value-added effect comparative test result:
Table 2
Claims (10)
1. a composite particles for oligoisomaltose and FOS, is characterized in that, component is as follows, is all weight percentage:
Oligoisomaltose 51%～99%, FOS 1%～49%.
2. composite particles as claimed in claim 1, is characterized in that, component is as follows, is all weight percentage:
Oligoisomaltose 60%～80%, FOS 20%～40%.
3. the preparation method of the composite particles of oligoisomaltose and FOS described in claim 1, is characterized in that, step is as follows:
(1) by oligoisomaltose liquid glucose through concentrated, make mass concentration and be 60～70% oligoisomaltose liquid,
(2) FOS liquid glucose warp is concentrated, dry, make FOS powder,
(3) the FOS powder that oligoisomaltose liquid step (1) being made and step (2) make adds in fluid bed granulator granulation in proportion, under 50～80 ℃ of temperature conditions, granulate, cross after 40～60 order vibratory sieves, make the composite particles of oligoisomaltose and FOS;
Or step is as follows:
(a) oligoisomaltose liquid glucose warp is concentrated, dry, make oligoisomaltose powder,
(b) by FOS liquid glucose through concentrated, make mass concentration and be 40～50% FOS liquid,
(c) the FOS liquid that oligoisomaltose powder step (a) being made and step (b) make adds in fluid bed granulator granulation in proportion; under 50～80 ℃ of temperature conditions, granulate; cross after 40～60 order vibratory sieves, make the composite particles of oligoisomaltose and FOS.
4. preparation method as claimed in claim 3, is characterized in that, described step (1) or (a) in the preparation method of oligoisomaltose liquid glucose as follows:
(I) regulating starch slurry concentration, after adjusting pH, add high temperature resistant AMS, is 20%～25% liquefier through liquefying, making DE value;
(II) in the liquefier making to step (I), add beta amylase, Pullulanase, fungi alpha amylase, carry out after saccharification react, then add phlorose to turn glycosides enzyme, proceed to turn glycosides reaction, the enzyme that then goes out is lived, and makes liquid glucose;
(III) in the liquid glucose making to step (II), add active carbon, after decolouring, with adverse current ion-exchange chromatography, carry out ion-exchange desalination, make desalination liquid glucose;
(IV) desalination liquid glucose step (III) being made is through chromatographic isolation, the eluent that collection contains isomaltose, panose and Isomaltotriose, makes the oligoisomaltose liquid glucose that isomaltose+panose in total reducing sugar+Isomaltotriose content is not less than 95wt%.
5. preparation method as claimed in claim 4, is characterized in that, in described step (I), starch slurry concentration is 15～20 Baume degrees, and pH value is 4～6.5, and the addition of high-temperatureα-amylase is that starch material per ton adds 1.2 * 10
7～2.0 * 10
7u, condensing temperature is 110～141 ℃; More excellent, described liquefaction is steam ejection liquefaction, liquefaction number of times is twice.
6. preparation method as claimed in claim 4, is characterized in that, in described step (II), the addition of described beta amylase is that starch material per ton adds 1.0 * 10
8～1.5 * 10
8the addition of U, Pullulanase is that starch material per ton adds 1.2 * 10
5～1.5 * 10
5the addition of ASPU, fungi alpha amylase is that starch material per ton adds 0.1 * 10
8～0.3 * 10
8u is saccharification react 2～5h under the condition of 50～60 ℃ in temperature; The addition that described phlorose turns glycosides enzyme is that starch material per ton adds 1.8 * 10
8～3.6 * 10
8u is under the condition of 55～60 ℃, to turn glycosides reaction 26～32h in temperature.
7. preparation method as claimed in claim 4, is characterized in that, in described step (III), the addition of active carbon is 0.3～0.5wt%, bleaching time 1.5～2.0h; Light transmittance >=85% of described desalination liquid glucose after ion-exchange desalination.
8. preparation method as claimed in claim 4, is characterized in that, in described step (IV), chromatographic isolation service condition is operating pressure 0.25～0.35MPa, 65～70 ℃ of temperature, water loss-rate 1:1.2～1:1.5, charging 1.5～1.7m per hour
3, collect the eluent that contains isomaltose, panose and Isomaltotriose, make the oligoisomaltose liquid glucose that isomaltose+panose in total reducing sugar+Isomaltotriose content is not less than 95wt%.
9. preparation method as claimed in claim 3, is characterized in that, described step (2) or (b) in the preparation method of FOS liquid glucose as follows:
(i) sucrose solution that preparation mass concentration is 25%～60%;
(ii) in the sucrose solution making to step (i), add β-transfructosylase, after reaction, make the thick solution of FOS;
(iii) step (ii) is made the thick solution of FOS through decolouring, filter, from handing over, chromatographic isolation, make the FOS liquid glucose that FOS content in total reducing sugar is not less than 95wt%.
10. preparation method as claimed in claim 9, is characterized in that, in described step (ii), β-transfructosylase originates from Aspergillus niger strain BLCY-02, and the addition of this enzyme is that sucrose per ton adds 0.5 * 10
6～1.5 * 10
6u, 20～40 ℃ of reaction temperatures, the reaction time is 20～36h;
Further preferred according to the present invention, in described step (iii), decolour for activated carbon decolorizing, active carbon addition 0.1～0.3wt%, bleaching time 1～1.5h; Be filtered into plate-frame filtering; Through solution light transmittance >=85% after friendship;
Further preferred according to the present invention, in described step (iii), chromatographic isolation service condition is operating pressure 0.2～0.35MPa, 60～70 ℃ of temperature, water loss-rate 1:(1.2～1.5), charging 1.8～2.0m per hour
3, collect ketose, Nystose, GF4.
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| CN105341746A (en) * | 2015-11-06 | 2016-02-24 | 滁州学院 | Nutrient purple potato ham sausage and processing technology thereof |
| CN106222216A (en) * | 2016-07-25 | 2016-12-14 | 山东百龙创园生物科技有限公司 | A kind of novel DP4 oligomeric isomaltose and preparation method thereof |
| CN107712888A (en) * | 2016-08-30 | 2018-02-23 | 河北农业大学 | A kind of raspberry oral liquid |
| CN109055461A (en) * | 2018-08-28 | 2018-12-21 | 广州双桥股份有限公司 | A kind of production method of oligoisomaltose |
| CN112111538A (en) * | 2020-09-21 | 2020-12-22 | 江南大学 | Functional sugar capable of adjusting positioning release of incretins and preparation method thereof |
| CN112438352A (en) * | 2019-08-15 | 2021-03-05 | 内蒙古伊利实业集团股份有限公司 | Oat beverage with low glucose content and preparation method thereof |
| CN114032262A (en) * | 2021-11-09 | 2022-02-11 | 山东星光首创生物科技有限公司 | Method for producing sucrose tetrasaccharide |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105341746A (en) * | 2015-11-06 | 2016-02-24 | 滁州学院 | Nutrient purple potato ham sausage and processing technology thereof |
| CN106222216A (en) * | 2016-07-25 | 2016-12-14 | 山东百龙创园生物科技有限公司 | A kind of novel DP4 oligomeric isomaltose and preparation method thereof |
| CN106222216B (en) * | 2016-07-25 | 2019-10-18 | 山东百龙创园生物科技股份有限公司 | A kind of DP4 oligoisomaltose and preparation method thereof |
| CN107712888A (en) * | 2016-08-30 | 2018-02-23 | 河北农业大学 | A kind of raspberry oral liquid |
| CN109055461A (en) * | 2018-08-28 | 2018-12-21 | 广州双桥股份有限公司 | A kind of production method of oligoisomaltose |
| CN109055461B (en) * | 2018-08-28 | 2021-12-17 | 广州双桥股份有限公司 | Production method of isomaltooligosaccharide |
| CN112438352A (en) * | 2019-08-15 | 2021-03-05 | 内蒙古伊利实业集团股份有限公司 | Oat beverage with low glucose content and preparation method thereof |
| CN112111538A (en) * | 2020-09-21 | 2020-12-22 | 江南大学 | Functional sugar capable of adjusting positioning release of incretins and preparation method thereof |
| CN112111538B (en) * | 2020-09-21 | 2022-05-10 | 江南大学 | Functional sugar capable of adjusting positioning release of incretins and preparation method thereof |
| CN114032262A (en) * | 2021-11-09 | 2022-02-11 | 山东星光首创生物科技有限公司 | Method for producing sucrose tetrasaccharide |
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