CN103555794A - isomaltose hypgather cleaning and production method - Google Patents
isomaltose hypgather cleaning and production method Download PDFInfo
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- CN103555794A CN103555794A CN201310481026.8A CN201310481026A CN103555794A CN 103555794 A CN103555794 A CN 103555794A CN 201310481026 A CN201310481026 A CN 201310481026A CN 103555794 A CN103555794 A CN 103555794A
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- semen maydis
- maydis powder
- oligomeric isomaltose
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- isomaltose hypgather
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Abstract
The invention belongs to the technical field of starch sugar and especially relates to an isomaltose hypgather cleaning and production method. The isomaltose hypgather cleaning and production method comprises the following steps: using peeled and degermed corn flour as a raw material, dissolving the corn flour by adding water, adding alpha-amylase in the dissolved solution, and adjusting pH value and keeping warm; cooling the dissolved solution and adding fungal enzyme for thermal insulation, adding alpha-transglucosidase and keeping warm, adjusting pH to neutral, and adding neutral protease to carry out enzymolysis so as to obtain an enzymatic hydrolysate; filtering, decolouring and refining the enzymatic hydrolysate and finally concentrating to obtain a concentrate; separating the concentrate by a sequential simulated moving bed, respectively collecting effluents of isomaltose hypgather, glucose and polysaccharide, concentrating the isomaltose hypgather eluant, and drying to obtain the product. The method provided by the invention has advantages of simple step and stable reaction, is easy to carry out, is adopted to effectively raise production efficiency and utilization rate of raw materials, reduce energy consumption and production cost and mitigate environmental burden, and is beneficial to industrial production.
Description
(1) technical field
The invention belongs to β-amylose technical field, particularly a kind of clean preparation method of oligomeric isomaltose.
(2) background technology
Oligomeric isomaltose be 2-8 glucose molecule with α-1, the oligosaccharides that 6 glycosidic links couple together; Generally, oligomeric isomaltose is not fermented by yeast, is non-fermentable oligose.Oligomeric isomaltose can effectively promote the growth and breeding of beneficial bacteria-bifidus bacillus in human body, therefore be called again " positive growth factor for bifidus ", is called for short " bifidus factor ".Through clinical for many years, show with practical application, bifidus bacillus has many nourishing functions, and promotes the oligomeric isomaltose of the factor naturally just to receive people's concern as bifidus bacillus.
Because oligomeric isomaltose has good processing characteristics, in various food, be applied to production technique without particular requirement, can add in food easily, and needn't worry that manufacturing procedure causes damage to it, thereby be widely used in food and healthcare products.Oligomeric isomaltose at candy, bake, have interpolation in functional drinks, milk-product, not only can not affect texture of food and mouthfeel, also strengthened the health-care effect of food.As apply the candy that oligomeric isomaltose is produced, there is the function of low in calories, unlikely carious tooth, resistive connection crystalline substance and whole intestines; In Puffer-type candy and burnt odor type candy, through beating for a long time inflation, formed heterogeneous spatial grid structure, aeration, gelation and retentiveness are all very strong, pliable and tough mainly with mouthfeel, have a chewiness.
At present, the raw materials for production of oligomeric isomaltose are mainly W-Gum or corn steep liquor, and traditional Corn Starch Wet complete processing, exists the shortcomings such as energy consumption is high, seriously polluted, and the comprehensive utilization ratio of corn is not high.Dry method Semen Maydis powder complete processing, the development potentiality of another approach ， China fully utilizing as China's corn resources is huge, and it can realize the total composition utilization of corn; Utilize Semen Maydis powder direct production oligomeric isomaltose can shorten the production chain of oligomeric isomaltose, reduce environmental pollution, reduce production costs.
(3) summary of the invention
The present invention, in order to make up the deficiencies in the prior art, provides the clean preparation method of the oligomeric isomaltose that a kind of step is simple, waste is few.
The present invention is achieved through the following technical solutions:
A clean preparation method for oligomeric isomaltose, the Semen Maydis powder of making through peeling, de-embryo of take is raw material, comprises the steps:
(1) Semen Maydis powder is dissolved in water, and in lysate, to add the α-amylase that accounts for Semen Maydis powder weight 0.06-0.1%, adjust pH be 5.5-6.0, be incubated 15-25min at 87-92 ℃;
(2) lysate is cooled to 40-50 ℃, add the fungal enzyme that accounts for Semen Maydis powder weight 0.1-0.2%, insulation 15-20h, add again the α-glucose transglucosidase that accounts for Semen Maydis powder weight 0.6-1%, 40-50 ℃ of insulation 10-15h, it is neutral adjusting pH, adds the neutral protease that accounts for Semen Maydis powder weight 1-2%, 35-40 ℃ of enzymolysis 2-4h, obtains enzymolysis solution;
(3) by enzymolysis solution filtration, decolouring, refining through continuous exchange of particles system, finally concentrate and to obtain concentrated solution;
(4) adopt and sequential simulated moving bed concentrated solution is carried out to separation, adopt highly acidic resin, charging flow velocity is 1.8-2.2L/h, feeding temperature is 60-70 ℃, separated pH value is 6.0-7.0, and be 15-25min switching time, collects respectively the effluent liquid of oligomeric isomaltose, glucose and polysaccharide, oligomeric isomaltose effluent liquid is concentrated, dry, obtain product.
The present invention be take Semen Maydis powder as raw material, adopt aborning neutral protease enzymolysis, can make the total macro-molecular protein of liquid glucose be decomposed into the proteins and peptides of small molecules solubility, do not affecting under the prerequisite of quality product, improve its impact and be worth, expand its use range.
The present invention adopts the separated mixed concentrated liquid of sequential type simulated moving bed chromatography, and by product glucose and polysaccharide effluent liquid are collected respectively, reaches the object of recycle, has improved product yield and economic worth simultaneously, has reduced cost.
More excellent scheme of the present invention is:
In step (3), it be 4-5 that described enzymolysis solution is adjusted pH value, adds flocculation agent, and standing insulation 40-50min at 70-80 ℃, then adds gac, stirring 20-30min, filtration enzymolysis solution.
Described Semen Maydis powder is low fat maize flour, and lipid content is less than or equal to 0.2%, and its fineness is 80-120 order.
In step (4), described simmer down to Multi-effect plate type vacuum evaporation, is dried as spraying dry.
Step of the present invention is simple, and stable reaction is easy to implement, effectively enhances productivity and raw-material utilization ratio, has reduced energy consumption and production cost, has alleviated environmental pressure, is conducive to suitability for industrialized production.
(4) embodiment
Embodiment:
The Semen Maydis powder of making through peeling, de-embryo of take is raw material, comprises the steps:
(1) Semen Maydis powder is dissolved in water, and in lysate, to add the α-amylase that accounts for Semen Maydis powder weight 0.06-0.1%, adjust pH be 5.5-6.0, be incubated 15-25min at 87-92 ℃;
(2) lysate is cooled to 40-50 ℃, add the fungal enzyme that accounts for Semen Maydis powder weight 0.1-0.2%, insulation 15-20h, add again the α-glucose transglucosidase that accounts for Semen Maydis powder weight 0.6-1%, 40-50 ℃ of insulation 10-15h, it is neutral adjusting pH, adds the neutral protease that accounts for Semen Maydis powder weight 1-2%, 35-40 ℃ of enzymolysis 2-4h, obtains enzymolysis solution;
(3) by enzymolysis solution filtration, decolouring, refining through continuous exchange of particles system, finally concentrate and to obtain concentrated solution; It is 4-5 that described enzymolysis solution is adjusted pH value, adds flocculation agent, and standing insulation 40-50min at 70-80 ℃, then adds gac, stirring 20-30min, filtration enzymolysis solution;
(4) adopt and sequential simulated moving bed concentrated solution is carried out to separation, adopt highly acidic resin, charging flow velocity is 1.8-2.2L/h, feeding temperature is 60-70 ℃, separated pH value is 6.0-7.0, and be 15-25min switching time, collects respectively the effluent liquid of oligomeric isomaltose, glucose and polysaccharide, oligomeric isomaltose effluent liquid is concentrated, dry, obtain product.
Described Semen Maydis powder is low fat maize flour, and lipid content is less than or equal to 0.2%, and its fineness is 80-120 order.
In step (4), described simmer down to Multi-effect plate type vacuum evaporation, is dried as spraying dry.
Claims (4)
1. the clean preparation method of an oligomeric isomaltose, the Semen Maydis powder of making through peeling, de-embryo of take is raw material, it is characterized by, comprise the steps: that (1) is dissolved in water Semen Maydis powder, and in lysate, add the α-amylase that accounts for Semen Maydis powder weight 0.06-0.1%, adjust pH is 5.5-6.0, is incubated 15-25min at 87-92 ℃; (2) lysate is cooled to 40-50 ℃, add the fungal enzyme that accounts for Semen Maydis powder weight 0.1-0.2%, insulation 15-20h, add again the α-glucose transglucosidase that accounts for Semen Maydis powder weight 0.6-1%, 40-50 ℃ of insulation 10-15h, it is neutral adjusting pH, adds the neutral protease that accounts for Semen Maydis powder weight 1-2%, 35-40 ℃ of enzymolysis 2-4h, obtains enzymolysis solution; (3) by enzymolysis solution filtration, decolouring, refining through continuous exchange of particles system, finally concentrate and to obtain concentrated solution; (4) adopt and sequential simulated moving bed concentrated solution is carried out to separation, adopt highly acidic resin, charging flow velocity is 1.8-2.2L/h, feeding temperature is 60-70 ℃, separated pH value is 6.0-7.0, and be 15-25min switching time, collects respectively the effluent liquid of oligomeric isomaltose, glucose and polysaccharide, oligomeric isomaltose effluent liquid is concentrated, dry, obtain product.
2. the clean preparation method of oligomeric isomaltose according to claim 1, is characterized in that: in step (3), it is 4-5 that described enzymolysis solution is adjusted pH value, add flocculation agent, standing insulation 40-50min at 70-80 ℃, then adds gac, stir 20-30min, filter enzymolysis solution.
3. the clean preparation method of oligomeric isomaltose according to claim 1, is characterized in that: described Semen Maydis powder is low fat maize flour, and lipid content is less than or equal to 0.2%, and its fineness is 80-120 order.
4. the clean preparation method of oligomeric isomaltose according to claim 1, is characterized in that: in step (4), described simmer down to Multi-effect plate type vacuum evaporation, is dried as spraying dry.
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| CN201310481026.8A CN103555794A (en) | 2013-10-15 | 2013-10-15 | isomaltose hypgather cleaning and production method |
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| CN201310481026.8A CN103555794A (en) | 2013-10-15 | 2013-10-15 | isomaltose hypgather cleaning and production method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103936878A (en) * | 2014-04-28 | 2014-07-23 | 黑龙江八一农垦大学 | Method for purifying isomaltose hypgather by virtue of sequential simulated moving chromatography (SSMB) |
| CN104171800A (en) * | 2014-08-08 | 2014-12-03 | 山东百龙创园生物科技有限公司 | Isomaltooligosacharide-fructooligosaccharide composite particle and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757990A (en) * | 2012-06-30 | 2012-10-31 | 保龄宝生物股份有限公司 | Preparation method of high-purity isomaltose hypgather |
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- 2013-10-15 CN CN201310481026.8A patent/CN103555794A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757990A (en) * | 2012-06-30 | 2012-10-31 | 保龄宝生物股份有限公司 | Preparation method of high-purity isomaltose hypgather |
Non-Patent Citations (1)
| Title |
|---|
| 刘宗利等: "模拟移动床色谱分离技术在功能糖生产中的应用", 《中国食品添加剂》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103936878A (en) * | 2014-04-28 | 2014-07-23 | 黑龙江八一农垦大学 | Method for purifying isomaltose hypgather by virtue of sequential simulated moving chromatography (SSMB) |
| CN104171800A (en) * | 2014-08-08 | 2014-12-03 | 山东百龙创园生物科技有限公司 | Isomaltooligosacharide-fructooligosaccharide composite particle and preparation method thereof |
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Application publication date: 20140205 |