CN107400687A - A kind of method of biology enzyme polymerization production oligoisomaltose - Google Patents
A kind of method of biology enzyme polymerization production oligoisomaltose Download PDFInfo
- Publication number
- CN107400687A CN107400687A CN201710800251.1A CN201710800251A CN107400687A CN 107400687 A CN107400687 A CN 107400687A CN 201710800251 A CN201710800251 A CN 201710800251A CN 107400687 A CN107400687 A CN 107400687A
- Authority
- CN
- China
- Prior art keywords
- oligoisomaltose
- liquid
- temperature
- feed liquid
- iii
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of method of biology enzyme polymerization production oligoisomaltose, comprise the following steps：(i) DE >=98% is prepared, the glucose concentrate that dry biomass content is 60~75%；(ii) pH value is adjusted 65~85 DEG C of temperature, to add Pullulanase 1.2~4NPUN/g butts to 4.0~6.5,1~10h of insulation reaction, enzyme deactivation, oligoisomaltose liquid is made glucose concentrate；(iii) by oligoisomaltose liquid through decolourizing, filter, from hand over, be concentrated in vacuo it is refined after, carry out chromatographic isolation, the final oligoisomaltose for obtaining high-purity.
Description
Technical field
The present invention relates to a kind of method of biology enzyme polymerization production oligoisomaltose, belongs to functional sugar manufacturing technology neck
Domain.
Background technology
Oligoisomaltose (Isomaltooligosacharide) is also known as bifurcation oligose, refers to glucosyl group with α -1,
The monose number that 6 glycosidic bonds are combined into is in 2~6 a kind of oligosaccharide not waited, because molecular conformation is different, to be different from maltose
And referred to as oligoisomaltose.
Oligoisomaltose has good physicochemical property and physiological function, is the 40%~50% of sucrose as sugariness is low,
And sweet taste is soft, mouthfeel is relatively refreshing；It is difficult to be digested by gastric enzyme, heat is low, does not increase blood pressure and blood lipoid substantially；Promote in human body intestinal canal
The propagation of Bifidobacterium, the formation of harmful bacteria and corrupt substance in enteron aisle can be suppressed.In addition, oligomeric different wheat sugar has anti-caries tooth
Performance etc..In view of the characteristic and healthcare function of oligoisomaltose, its application is quite varied, may apply to food, doctor
The industries such as medicine, feed, cosmetics.
Contain more macromolecular branch polysaccharide in oligoisomaltose obtained by domestic traditional processing technology at present, effective three
Sugared (isomaltose, panose, Isomaltotriose) content only 45%, Physiological Properties are weaker, it is necessary to which 20~30 grams/day of ability show
Reveal obvious effect, and osmotic pressure is relatively low, water activity is high, is unfavorable for the preservation storage of food.
Chinese patent literature CN104152512A (application number 201410390546.2) discloses a kind of oligoisomaltose
Preparation method, comprise the following steps：(1) water is added into starch material to size mixing concentration, high temperature resistant α-shallow lake is added after adjusting pH
Powder enzyme, starch slurry is made；(2) by starch slurry after liquefaction, liquefier is made；(3) maltotriose generation will be added in liquefier
Enzyme and Pullulanase, after insulation saccharification, enzyme deactivation is lived, and saccharified liquid is made；(4) alpha-glucosaccharase transferase is added into saccharified liquid,
Reaction, which is made, once turns glycosides liquid glucose；Then again plus time enter alpha-glucosaccharase transferase, after reaction, enzyme deactivation live, be made turn glycosides liquid glucose；
(5) will turn glycosides liquid glucose through decolourizing, filter, from friendship, chromatographic isolation, concentration and dry after, be made oligoisomaltose.
Above-mentioned technical proposal carries out maltose generation using maltotriose generation enzyme and Pullulanase, then using α-grape
Glucoside transferase prepares oligoisomaltose, although high-purity oligoisomaltose can be obtained, yield is not high, prepares cost
It is higher.
The content of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of method of biology enzyme polymerization production oligoisomaltose.
Term explanation
NPUN(New Pullulanase Unit Novo)：It is new inscribe Pullulanase unit of activity, is believed with Novi
Determined on the basis of Promozyme D standard enzymes；1NPUN=0.35PUN；
One Pullulanase unit of activity (PUN)：The release per minute under standard conditions as defined in method KAL-SM-1013
Locate the amount of the enzyme needed for 1 μm of ol glucose.
In order to realize the purpose of the present invention, the present invention adopts the following technical scheme that：
A kind of method of biology enzyme polymerization production oligoisomaltose, comprises the following steps：
(i) DE >=98% is prepared, the glucose concentrate that dry biomass content is 60~75%；
(ii) pH value is adjusted 65~85 DEG C of temperature, to add general Shandong to 4.0~6.5 glucose concentrate made from step (i)
Blue 1.2~4NPUN/g of enzyme butts, 1~10h of insulation reaction, enzyme deactivation, oligoisomaltose liquid is made；
After testing, effective three sugared content of gained oligoisomaltose liquid (isomaltose, panose, Isomaltotriose) content is
20~35%；
(iii) by oligoisomaltose liquid made from step (ii) through decolourizing, filter, from hand over, be concentrated in vacuo it is refined after, enter
Row chromatographic isolation, the final oligoisomaltose for obtaining high-purity.
After testing, effective three sugared content of gained oligoisomaltose liquid (isomaltose, panose, Isomaltotriose) content >=
95%.
According to currently preferred, in the step (i), the preparation process of glucose concentrate is as follows：
(1) it is 14~20 ° of Be to add water to size mixing to concentration into starch, adjusts pH value that starch slurry is made to 5.5~6.5；
(2) Thermostable α-Amylase is added into starch slurry made from step (1), the addition of fire resistant alpha-diastase is every
Gram starch (dry weight) adds 10~15U fire resistant alpha-diastases, after the once injection liquefaction of 108~110 DEG C of temperature, then through temperature
123~125 DEG C of secondary injection liquefaction, is made liquefier；
(3) liquefier made from step (2) is cooled to 50~60 DEG C, adjusts pH value to 4.5~5.2, it is (dry by every gram of starch
Weight) 100~200U amount add saccharification 24~48h of enzymatic conversion, enzyme deactivation live, be made saccharified liquid；
After testing, glucose content >=95% in obtained saccharified liquid；
(4) DE >=98%, dry is made through decolourizing, filtering, from handing over, being concentrated in vacuo in saccharified liquid made from step (3)
Mass content is 60-75% glucose concentrate.
According to the present invention it is further preferred that the temperature that enzyme deactivation is lived in the step (3) is 80~90 DEG C.
According to the present invention it is further preferred that being decolourized in the step (4) for by weight percent 1.0%~1.5%
Ratio adds activated carbon, under agitation, carries out 30~60min of decolorization.
Filtered according to the present invention it is further preferred that being filtered into the step (4) using flame filter press.
According to the present invention it is further preferred that expecting in the step (4) from friendship to be handled using continuous ionic exchange system
Liquid, from feed liquid light transmittance >=98% after friendship.Feed liquid after processing is as clear as crystal, free from extraneous odour.
According to currently preferred, Pullulanase addition is 2.0~3.5NPUN/g butts in the step (ii).
According to ratio currently preferred, that the middle decolouring of the step (iii) is by weight percent 0.5%~1.0%
Activated carbon is added, under agitation, carries out 10~30min of decolorization.
According to currently preferred, in the step (iii), chromatrographic separation step is as follows：
Volume will be concentrated into from feed liquid after friendship as after 50~60%, into chromatographic fractionation system, chromatographic run pressure 0.25
~0.35MPa, 65~75 DEG C of temperature, water consume is than 1:(1.2~1.4), 1.5~2.0m is fed per hour3, collect oligomeric different malt
Sugar.
According to currently preferred, in the step (iii), it is filtered into and is filtered using flame filter press.
It is that feed liquid is handled using continuous ionic exchange system from handing in the step (iii) according to currently preferred,
From feed liquid light transmittance >=98% after friendship.Feed liquid after processing is as clear as crystal, free from extraneous odour.
Beneficial effect
The present invention realizes the polymerization to glucose using Pullulanase under given conditions first, produces oligomeric different malt
Sugar, effectively trisaccharide (isomaltose, panose and Isomaltotriose) content is up to more than 98% in obtained product, no macromolecular point
Branch polysaccharide, Physiological Properties are very strong, it is only necessary to which 5-10 grams, be 3 times of traditional product；Simultaneously because because more without macromolecular
Sugar, osmotic pressure improve, and water activity is greatly lowered, and greatly extends shelf life.
Brief description of the drawings
The testing result peak value figure of oligoisomaltose liquid made from Fig. 1 embodiments 3.
Embodiment
Technical scheme is further elaborated with reference to embodiment, but institute's protection domain of the present invention is not limited to
This.
Thermostable α-Amylase is purchased from Jie Nengke bioengineering Co., Ltd, and enzyme activity is enzyme activity 20000U/ml；
Carbohydrase is ordinary commercial products.
Pullulanase is purchased from Novozymes Company, vigor 4000NPUN/g.
Embodiment 1
A kind of method of biology enzyme polymerization production oligoisomaltose, comprises the following steps：
(1) it is 17 ° of Be to add water to size mixing to concentration into starch, adjusts pH value that starch slurry is made to 6.0；
(2) Thermostable α-Amylase is added into starch slurry made from step (1), the addition of fire resistant alpha-diastase is every
Gram starch (dry weight) adds 12U fire resistant alpha-diastases, after the once injection liquefaction of 109 DEG C of temperature, then two through 124 DEG C of temperature
Secondary injection liquefaction, is made liquefier；
(3) liquefier made from step (2) is cooled to 55 DEG C, pH value is adjusted to 4.8, by every gram of starch (dry weight) 150U's
Amount adds saccharification enzymatic conversion 36h, and 85 DEG C of enzyme deactivations are lived, and saccharified liquid is made；
After testing, glucose content is 95.1% in obtained saccharified liquid.
(4) 1.2% ratio adds activated carbon by weight percent in saccharified liquid made from item step (3), in stirring bar
Under part, decolorization 45min is carried out, is then filtered using flame filter press, feed liquid is handled through continuous ionic exchange system,
After feed liquid light transmittance >=98% after friendship, it is concentrated in vacuo, it is dense for 68% glucose that DE >=98%, dry biomass content is made
Contracting liquid；
(5) pH value is adjusted 75 DEG C of temperature, to add Pullulanase to 5.2 glucose concentrate made from step (4)
2.6NPUN/g butts, insulation reaction 5.5h, enzyme deactivation, oligoisomaltose liquid is made；
After testing, effective three sugared content of gained oligoisomaltose liquid (isomaltose, panose, Isomaltotriose) content is
20%；
(6) into oligoisomaltose liquid made from step (5), 0.7% ratio adds activated carbon by weight percent,
Under agitation, decolorization 20min is carried out, flame filter press is filtered, continuous ionic exchange system processing feed liquid, from
Feed liquid light transmittance >=98% after friendship, after being concentrated in vacuo and refining, carry out chromatographic isolation, the final oligomeric different malt for obtaining high-purity
Sugar；
Chromatrographic separation step is as follows：
Volume will be concentrated into from feed liquid after friendship as after 55%, into chromatographic fractionation system, chromatographic run pressure 0.3MPa, temperature
70 DEG C of degree, water consume is than 1:1.3,1.75m is fed per hour3, collect oligoisomaltose.
After testing, effective three sugared content of gained oligoisomaltose liquid (isomaltose, panose, Isomaltotriose) content is
98.3%, yield 93.5%.
Embodiment 2
A kind of method of biology enzyme polymerization production oligoisomaltose, comprises the following steps：
(1) it is 14 ° of Be to add water to size mixing to concentration into starch, adjusts pH value that starch slurry is made to 6.5；
(2) Thermostable α-Amylase is added into starch slurry made from step (1), the addition of fire resistant alpha-diastase is every
Gram starch (dry weight) adds 10U fire resistant alpha-diastases, after the once injection liquefaction of 110 DEG C of temperature, then two through 123 DEG C of temperature
Secondary injection liquefaction, is made liquefier；
(3) liquefier made from step (2) is cooled to 60 DEG C, pH value is adjusted to 4.5, by every gram of starch (dry weight) 200U's
Amount adds saccharification enzymatic conversion 24h, and 90 DEG C of enzyme deactivations are lived, and saccharified liquid is made；
After testing, glucose content is 95%.3 in obtained saccharified liquid.
(4) 1.0% ratio adds activated carbon by weight percent in saccharified liquid made from item step (3), in stirring bar
Under part, decolorization 60min is carried out, is then filtered using flame filter press, feed liquid is handled through continuous ionic exchange system,
After feed liquid light transmittance >=98% after friendship, it is concentrated in vacuo, it is dense for 60% glucose that DE >=98%, dry biomass content is made
Contracting liquid；
(5) pH value is adjusted 65 DEG C of temperature, to add Pullulanase 4NPUN/ to 6.5 glucose concentrate made from step (4)
G butts, insulation reaction 1h, enzyme deactivation, oligoisomaltose liquid is made；
After testing, effective three sugared content of gained oligoisomaltose liquid (isomaltose, panose, Isomaltotriose) content is
28%；
(6) into oligoisomaltose liquid made from step (5), 1.0% ratio adds activated carbon by weight percent,
Under agitation, decolorization 10min is carried out, flame filter press is filtered, continuous ionic exchange system processing feed liquid, from
Feed liquid light transmittance >=98% after friendship, after being concentrated in vacuo and refining, carry out chromatographic isolation, the final oligomeric different malt for obtaining high-purity
Sugar；
Chromatrographic separation step is as follows：
Volume will be concentrated into from feed liquid after friendship as after 60%, into chromatographic fractionation system, chromatographic run pressure 0.25MPa,
75 DEG C of temperature, water consume is than 1:1.2,2.0m is fed per hour3, collect oligoisomaltose.
After testing, effective three sugared content of gained oligoisomaltose liquid (isomaltose, panose, Isomaltotriose) content is
98.5%, yield 91.5%.
Embodiment 3
A kind of method of biology enzyme polymerization production oligoisomaltose, comprises the following steps：
(1) it is 20 ° of Be to add water to size mixing to concentration into starch, adjusts pH value that starch slurry is made to 5.5；
(2) Thermostable α-Amylase is added into starch slurry made from step (1), the addition of fire resistant alpha-diastase is every
Gram starch (dry weight) adds 15U fire resistant alpha-diastases, after the once injection liquefaction of 108 DEG C of temperature, then two through 125 DEG C of temperature
Secondary injection liquefaction, is made liquefier；
(3) liquefier made from step (2) is cooled to 50 DEG C, pH value is adjusted to 5.2, by every gram of starch (dry weight) 100U's
Amount adds saccharification enzymatic conversion 48h, and 80 DEG C of enzyme deactivations are lived, and saccharified liquid is made；
After testing, glucose content is 95.5% in obtained saccharified liquid；
(4) 1.5% ratio adds activated carbon by weight percent in saccharified liquid made from item step (3), in stirring bar
Under part, decolorization 30min is carried out, is then filtered using flame filter press, feed liquid is handled through continuous ionic exchange system,
After feed liquid light transmittance >=98% after friendship, it is concentrated in vacuo, it is dense for 75% glucose that DE >=98%, dry biomass content is made
Contracting liquid；
(5) pH value is adjusted 85 DEG C of temperature, to add Pullulanase to 4.0 glucose concentrate made from step (4)
1.2NPUN/g butts, insulation reaction 10h, enzyme deactivation, oligoisomaltose liquid is made；
After testing, effective three sugared content of gained oligoisomaltose liquid (isomaltose, panose, Isomaltotriose) content is
29%；
(6) into oligoisomaltose liquid made from step (5), 0.5% ratio adds activated carbon by weight percent,
Under agitation, decolorization 30min is carried out, flame filter press is filtered, continuous ionic exchange system processing feed liquid, from
Feed liquid light transmittance >=98% after friendship, after being concentrated in vacuo and refining, carry out chromatographic isolation, the final oligomeric different malt for obtaining high-purity
Sugar；
Chromatrographic separation step is as follows：
Volume will be concentrated into from feed liquid after friendship as after 50%, into chromatographic fractionation system, chromatographic run pressure 0.35MPa,
65 DEG C of temperature, water consume is than 1:1.4,1.5m is fed per hour3, collect oligoisomaltose.
After testing, effective three sugared content of gained oligoisomaltose liquid (isomaltose, panose, Isomaltotriose) content is
98.6%, as shown in figure 1, yield is 92%.
Embodiment 4
The method of biology enzyme polymerization production oligoisomaltose as described in Example 3, difference are：
(5) pH value is adjusted 85 DEG C of temperature, to add Pullulanase to 4.0 glucose concentrate made from step (4)
2.0NPUN/g butts, insulation reaction 10h, enzyme deactivation, oligoisomaltose liquid is made；
After testing, effective three sugared content of gained oligoisomaltose liquid (isomaltose, panose, Isomaltotriose) content is
32%；
Obtained effective three sugared content of oligoisomaltose liquid (isomaltose, panose, Isomaltotriose) content is
98.8%, yield 93%.
Embodiment 5
The method of biology enzyme polymerization production oligoisomaltose as described in Example 3, difference are：
(5) pH value is adjusted 85 DEG C of temperature, to add Pullulanase to 4.0 glucose concentrate made from step (4)
3.5NPUN/g butts, insulation reaction 10h, enzyme deactivation, oligoisomaltose liquid is made；
After testing, effective three sugared content of gained oligoisomaltose liquid (isomaltose, panose, Isomaltotriose) content is
35%；
Obtained effective three sugared content of oligoisomaltose liquid (isomaltose, panose, Isomaltotriose) content >=99%,
Yield is 93.2%.
Comparative example 1
Method according to Chinese patent literature CN104152512A (application number 201410390546.2) embodiment 3 prepares low
IMO.
After testing, effective three sugared content of gained oligoisomaltose liquid (isomaltose 12%, panose 20%, different malt three
15%) content is 47% to sugar, yield 40%.
Comparative example 2
Method as described in Example 1, difference are that the condition of step (5) is as follows：
PH value is adjusted 85 DEG C of temperature, to add Pullulanase 0.3NPUN/g to 4.0 glucose concentrate made from step (4)
Butt, insulation reaction 10h, enzyme deactivation, oligoisomaltose liquid is made；
After testing, effective three sugared content of gained oligoisomaltose liquid (isomaltose, panose, Isomaltotriose) content is
3.5%.
Interpretation of result
The addition that Pullulanase is can be seen that by the data of comparative example 1 and comparative example 2 is to determine efficiency of pcr product
Key parameter.Those skilled in the art know that existing Pullulanase is generally used as debranching enzyme, but inventor is led to
Cross research to find, under special reaction condition, Pullulanase can realize the effect to glucose polymerisation and then generation effective three
Sugar.
Claims (10)
- A kind of 1. method of biology enzyme polymerization production oligoisomaltose, it is characterised in that comprise the following steps：(i) DE >=98% is prepared, the glucose concentrate that dry biomass content is 60~75%；(ii) pH value is adjusted 65~85 DEG C of temperature, to add Pullulanase to 4.0~6.5 glucose concentrate made from step (i) 1.2~4NPUN/g butts, 1~10h of insulation reaction, enzyme deactivation, oligoisomaltose liquid is made；(iii) by oligoisomaltose liquid made from step (ii) through decolourizing, filter, from hand over, be concentrated in vacuo it is refined after, carry out color Spectrum separation, the final oligoisomaltose for obtaining high-purity.
- 2. the method as described in claim 1, it is characterised in that in the step (i), the preparation process of glucose concentrate is such as Under：(1) it is 14~20 ° of Be to add water to size mixing to concentration into starch, adjusts pH value that starch slurry is made to 5.5~6.5；(2) Thermostable α-Amylase is added into starch slurry made from step (1), the addition of fire resistant alpha-diastase is every gram of shallow lake Powder (dry weight) adds 10~15U fire resistant alpha-diastases, after 108~110 DEG C of once injection of temperature is liquefied, then through temperature 123 ~125 DEG C of secondary injection liquefaction, is made liquefier；(3) liquefier made from step (2) is cooled to 50~60 DEG C, pH value is adjusted to 4.5~5.2, by every gram of starch (dry weight) 100~200U amount adds saccharification 24~48h of enzymatic conversion, and enzyme deactivation is lived, and saccharified liquid is made；(4) DE >=98%, dry biomass is made through decolourizing, filtering, from handing over, being concentrated in vacuo in saccharified liquid made from step (3) Content is 60-75% glucose concentrate.
- 3. method as claimed in claim 2, it is characterised in that the temperature that enzyme deactivation is lived in the step (3) is 80~90 DEG C.
- 4. method as claimed in claim 2, it is characterised in that decolourize in the step (4) for by weight percent 1.0%~ 1.5% ratio adds activated carbon, under agitation, carries out 30~60min of decolorization.
- 5. method as claimed in claim 2, it is characterised in that be filtered into the step (4) and carried out using flame filter press Filtering；Preferably, it is that feed liquid is handled using continuous ionic exchange system from handing in the step (4), from feed liquid light transmittance after friendship >=98%.
- 6. the method as described in claim 1, it is characterised in that in the step (ii) Pullulanase addition be 2.0~ 3.5NPUN/g butt.
- 7. the method as described in claim 1, it is characterised in that decolourized in the step (iii) for by weight percent 0.5% ~1.0% ratio adds activated carbon, under agitation, carries out 10~30min of decolorization.
- 8. the method as described in claim 1, it is characterised in that in the step (iii), chromatrographic separation step is as follows：Volume will be concentrated into from feed liquid after friendship as after 50~60%, into chromatographic fractionation system, chromatographic run pressure 0.25~ 0.35MPa, 65~75 DEG C of temperature, water consume is than 1:(1.2~1.4), 1.5~2.0m is fed per hour3, collect oligomeric different malt Sugar.
- 9. the method as described in claim 1, it is characterised in that in the step (iii), be filtered into and entered using flame filter press Row filtering.
- 10. the method as described in claim 1, it is characterised in that be to be handed over using continuous ionic from handing in the step (iii) System processing feed liquid is changed, from feed liquid light transmittance >=98% after friendship.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710800251.1A CN107400687A (en) | 2017-09-07 | 2017-09-07 | A kind of method of biology enzyme polymerization production oligoisomaltose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710800251.1A CN107400687A (en) | 2017-09-07 | 2017-09-07 | A kind of method of biology enzyme polymerization production oligoisomaltose |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107400687A true CN107400687A (en) | 2017-11-28 |
Family
ID=60397089
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710800251.1A Pending CN107400687A (en) | 2017-09-07 | 2017-09-07 | A kind of method of biology enzyme polymerization production oligoisomaltose |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107400687A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628843A (en) * | 2019-10-29 | 2019-12-31 | 保龄宝生物股份有限公司 | Preparation process of oligomeric maltose syrup with maltotetraose content of more than or equal to 60 percent |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101446489B1 (en) * | 2014-06-05 | 2014-10-01 | 농업회사법인 오르피아 주식회사 | Method for Manufacturing High Purity Isomalto-oligosaccharide Syrup Using Organic Rice |
| CN104131051A (en) * | 2014-08-08 | 2014-11-05 | 山东百龙创园生物科技有限公司 | Preparation method of isomaltooligosaccharide |
| CN104152512A (en) * | 2014-08-08 | 2014-11-19 | 山东百龙创园生物科技有限公司 | Preparation method of isomaltooligosacharide |
| CN105671105A (en) * | 2016-04-11 | 2016-06-15 | 江南大学 | Method for producing malt syrup and isomaltooligosaccharide in enzyme-adding, extruding and liquefying mode |
-
2017
- 2017-09-07 CN CN201710800251.1A patent/CN107400687A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101446489B1 (en) * | 2014-06-05 | 2014-10-01 | 농업회사법인 오르피아 주식회사 | Method for Manufacturing High Purity Isomalto-oligosaccharide Syrup Using Organic Rice |
| CN104131051A (en) * | 2014-08-08 | 2014-11-05 | 山东百龙创园生物科技有限公司 | Preparation method of isomaltooligosaccharide |
| CN104152512A (en) * | 2014-08-08 | 2014-11-19 | 山东百龙创园生物科技有限公司 | Preparation method of isomaltooligosacharide |
| CN105671105A (en) * | 2016-04-11 | 2016-06-15 | 江南大学 | Method for producing malt syrup and isomaltooligosaccharide in enzyme-adding, extruding and liquefying mode |
Non-Patent Citations (3)
| Title |
|---|
| TAKASHI KURIKI等: "A New Way of Producing Isomalto-Oligosaccharide Syrup by Using the Transglycosylation Reaction of Neopullulanase", 《APPL ENVIRON MICROBIOL.》 * |
| 尤新: "《淀粉糖品生产与应用手册》", 30 June 1997, 中国轻工业出版社 * |
| 黄金莲等: "普鲁兰酶对不同原料淀粉糖生产的影响", 《安徽农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628843A (en) * | 2019-10-29 | 2019-12-31 | 保龄宝生物股份有限公司 | Preparation process of oligomeric maltose syrup with maltotetraose content of more than or equal to 60 percent |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Aiyer | Amylases and their applications | |
| CN104152512B (en) | Preparation method of isomaltooligosacharide | |
| JP7011393B2 (en) | Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzyme | |
| CN104561191B (en) | A kind of preparation method of resistant dextrin | |
| CN104131051B (en) | A kind of preparation method of oligoisomaltose | |
| Narisetty et al. | Valorization of renewable resources to functional oligosaccharides: Recent trends and future prospective | |
| CN102321704B (en) | Method for treating starchy raw material and method for preparing citric acid | |
| CN104212854A (en) | Method for producing isomalto-oligosaccharide by using high-concentration starch | |
| CN107043796B (en) | A method of preparing malt syrup | |
| CN105950686A (en) | Novel use of maltotriosyl transferase | |
| KR101446489B1 (en) | Method for Manufacturing High Purity Isomalto-oligosaccharide Syrup Using Organic Rice | |
| CN105925642A (en) | Method of industrial production for trehalose by way of microbial fermentation | |
| CN106244647A (en) | A kind of method simultaneously preparing trehalose and gluconic acid lactone | |
| CN104204216A (en) | Method for making high maltose syrup | |
| CN108546724A (en) | High-purity oligoisomaltose and preparation method thereof | |
| CN104164458A (en) | Processing method of starch raw material and method for preparing citric acid | |
| Power | Enzymatic conversion of starch to fermentable sugars | |
| CN104718279A (en) | Method for preparing fermented ginkgo biloba beverage using heat-resistant amylase | |
| CN108300749A (en) | A method of preparing straight chain maltopentaose with two enzymes method | |
| CN101438782B (en) | Moisture-keeping syrup and preparation method thereof | |
| Samanta | Structural and Catalytical Features of Different Amylases and their Potential Applications. | |
| CN102234672A (en) | Enzymolysis method for starchy material and method for preparing citric acid | |
| CN107400687A (en) | A kind of method of biology enzyme polymerization production oligoisomaltose | |
| TWI284151B (en) | Methods of preparing starch-derived products | |
| WO2002010427A1 (en) | Method for producing maltose syrup by using a hexosyltransferase |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 251200 Dexin Street, Dezhou (Yucheng) National High-tech Industrial Development Zone, Shandong Province Applicant after: Shandong Bailong Park biological Polytron Technologies Inc Address before: 251200 Dexin Street, Yucheng High-tech Development Zone, Dezhou City, Shandong Province Applicant before: Shandong Bailong Park biological Polytron Technologies Inc |
|
| CB02 | Change of applicant information |