CN102321704B - Method for treating starchy raw material and method for preparing citric acid - Google Patents
Method for treating starchy raw material and method for preparing citric acid Download PDFInfo
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Abstract
The invention relates to a method for treating a starchy raw material and a method for preparing citric acid. The method for treating the starchy raw material comprises the following steps of: mixing powder of the starchy raw material and water to obtain farinaceous size, and mixing the farinaceous size and first part amylase to obtain a mixture; performing primary injection on the mixture by using first steam at the temperature of between 80 and 100 DEG C to obtain primary emulsion, and performing secondary injection on the primary emulsion by using second steam at the temperature of between 120 and 150 DEG C to obtain secondary emulsion; performing flash evaporation and reducing the temperature; and mixing a product obtained through flash evaporation and temperature reduction and second part amylase, and performing enzymolysis. By the method, a dextrose equivalent (DE) value can be reduced, and the fermentation period of the citric acid can be shortened.
Description
Technical field
The present invention relates to a kind for the treatment of process of starchy material and the preparation method of citric acid.
Background technology
Citric acid is a kind of organic acid that is widely used in the industries such as beverage, food and medicine.At present, citric acid mainly prepares by fermentation method, generally first starchy material is pulverized, product and water after pulverizing are mixed to get farinaceous size, farinaceous size is mixed with enzyme carry out enzymolysis, obtain enzymolysis product (enzymatic liquefaction liquid), and aspergillus niger is seeded to fermentation generation citric acid in the fermented liquid that contains described enzymolysis product.
In above-mentioned technique, the enzymolysis of starchy material (liquefaction) is larger on the fermentation period impact of citric acid.Present starchy material enzymolysis (liquefaction) technique adopts steam ejection liquefaction technique mostly, and namely farinaceous size is injected into certain temperature liquefaction after enzyme-added, then separates after flash distillation, cooling, and isolated liquid glucose enters fermentation system.
There is following defective in this technique:
(1) gelatinization is not thorough, after the starchy material particle water-swelling of many greater particle sizes, only the surface is by Starch Hydrolysis, the above particle of 0.85mm (be equivalent to 20 mesh sieve hole) for example, only have the noncrystalline domain of starch and the crystal region swelling on surface, inner crystal region is difficult to reach gelatinization.
(2) liquefaction is not thorough, and amylase is difficult to and there is no the starch crystal region effect of swelling, although added many amylase, starchy material still is difficult to liquefaction, in order to make the iodine examination qualified, just must add amylase more, causes diastatic waste.
(3) the DE value of liquefier (reducing sugar (with glucose meter) accounts for the degree of syrup dry) is higher, under similarity condition, the DE value increases with diastatic increase, the DE value of the liquefaction clear liquid that this method is obtained is 28-30%, cause the dextrin molecule too little, and the DE value scope that is beneficial to citric acid fermentation is at 15-20%.Be unfavorable for the saccharifying enzyme effect.Therefore, need the treatment process of a kind of starchy material of exploitation, be applied to citric acid fermentation.
Summary of the invention
The treatment process that the purpose of this invention is to provide a kind of starchy material adopts the enzyme-added second spraying technique of secondary, overcomes the defects that exists in prior art.
To achieve these goals, the invention provides a kind for the treatment of process of starchy material, the method comprises the steps:
(1) starchy material powder and water are mixed to get farinaceous size, farinaceous size is mixed with first part amylase, obtain mixture;
(2) mixture that obtains in step (1) is once sprayed under 80-100 ℃ with the first steam, obtain liquefier one time, then a liquefier is carried out second spraying with the second steam under 120-150 ℃, obtain the secondary liquefier;
(3) the secondary liquefier that obtains in step (2) is carried out flash distillation and cooling;
(4) under the amylorrhexis condition, the product after the flash distillation that step (3) is obtained and cooling mixes with second section amylase, carries out enzymolysis.
The present invention also provides a kind of preparation method of citric acid, and the method comprises according to aforesaid method to be processed starchy material, obtains enzymolysis product, and this enzymolysis product is fermented under aspergillus niger exists.
By technique scheme, the enzyme-added second spraying technique of secondary makes the DE value of liquefier lower, is conducive to the aspergillus niger saccharification, reduces the fermentation residual sugar, shortens fermentation period, improves fermentation level; Second spraying makes liquefaction more thorough simultaneously, has reduced the residual starch content in the enzymolysis residue, has improved the sugaring yield, has reduced the grain consumption.Secondary is enzyme-added has guaranteed diastatic activity simultaneously.
Other features and advantages of the present invention will partly be described in detail in embodiment subsequently.
Embodiment
Below the specific embodiment of the present invention is elaborated.Should be understood that, embodiment described herein only is used for description and interpretation the present invention, is not limited to the present invention.
The invention provides a kind for the treatment of process of starchy material, the method comprises the steps:
(1) starchy material powder and water are mixed to get farinaceous size, farinaceous size is mixed with first part amylase, obtain mixture;
(2) mixture that obtains in step (1) is once sprayed under 80-100 ℃ with the first steam, obtain liquefier one time, then a liquefier is carried out second spraying with the second steam under 120-150 ℃, obtain the secondary liquefier;
(3) the secondary liquefier that obtains in step (2) is carried out flash distillation and cooling;
(4) under the amylorrhexis condition, the product after the flash distillation that step (3) is obtained and cooling mixes with second section amylase, carries out enzymolysis.
Amylase refers to the general name of class of enzymes that can the starch-splitting glycosidic link, and described amylase generally comprises α-amylase, beta-amylase, saccharifying enzyme and isoamylase.
α-amylase claims again starch Isosorbide-5-Nitrae-dextrinase, and it can cut the α-Isosorbide-5-Nitrae of starch chain inside-glycosidic link at random, brokenly, and Starch Hydrolysis is maltose, contains the oligosaccharides of 6 glucose units and with the oligosaccharides of side chain.
Beta-amylase claims again starch Isosorbide-5-Nitrae-maltoside enzyme, can cut Isosorbide-5-Nitrae-glycosidic link from the starch molecule non reducing end, generates maltose.The product that this enzyme acts on starch is maltose and limit dextrin.
Saccharifying enzyme claims again starch α-Isosorbide-5-Nitrae-glucuroide, and this enzyme acts on the non reducing end of starch molecule, take glucose as unit, acts on successively α-Isosorbide-5-Nitrae in starch molecule-glycosidic link, generates glucose.Saccharifying enzyme acts on that product after amylopectin has glucose and with α-1, the oligosaccharides of 6-glycosidic link; The product that acts on after amylose starch is all almost glucose.
Isoamylase claims again starch α-1, and 6-glucuroide, branching enzyme, this enzyme act on the α-1 at amylopectin molecule branching-point place, and the 6-glycosidic link downcuts the whole side chain of amylopectin and becomes amylose starch.
According to the present invention, preferably use α-amylase and/or isoamylase.More preferably use high temperature resistant α-amylase.High temperature resistant α-amylase has excellent heat resistance, to adopt Bacillus licheniformis to cultivate through deep layer, the operations such as extraction are refining to be formed, can random hydrolysis the α-1 of starch, glycogen and degradation product inside thereof, 4 glucosides are strong makes the viscosity of gluey starch solution descend rapidly, produce soluble dextrins and oligosaccharide, excessive hydrolysis can produce a small amount of glucose and maltose.
The present inventor's discovery, the pH value of controlling well when the starchy material powder is sized mixing are further to control the preferred version of DE value well, may be the reasons due to the zymin correlation properties.Therefore, under preferable case of the present invention, the condition that described starchy material powder mixes with water comprises: temperature is 45-60 ℃, and the pH value is controlled at 5.5-6.2, and the weight ratio of starchy material powder and water is 1: 1.8-4.The condition that further preferred described starchy material powder mixes with water comprises: temperature is 50-55 ℃, and the pH value is controlled at 5.6-6, and the weight ratio of starchy material powder and water is 1: 2-3.
More abundant for enzymolysis, under preferable case, in step (1), the condition that farinaceous size mixes with first part amylase comprises: temperature is 45-60 ℃, and the time is 30-60 minute.Under preferable case, in step (4), described enzymatic hydrolysis condition comprises: hydrolysis temperature is 85-99 ℃, and enzymolysis time is 60-150 minute.
In addition, the present inventor also finds, under equal processing condition, the enzyme concentration of controlling well is also further to control the preferred version of DE well.Therefore, under preferable case of the present invention, with the dry weight basis of every gram starchy material powder, described diastatic total consumption is the 8-24 enzyme activity unit.10-20 enzyme activity unit more preferably.The diastatic consumption of described first part and the adjustable a wider range of the diastatic consumption of second section, comprehensive hydrolysis result and cost consideration, the diastatic consumption of preferred described first part is the 20-50 % by weight of diastatic total consumption, and the diastatic consumption of described second section is the 50-80 % by weight of diastatic total consumption.Further the diastatic consumption of preferred described first part is the 30-40 % by weight of diastatic total consumption, and the diastatic consumption of described second section is the 60-70 % by weight of diastatic total consumption.
Define according to GB 8275-2009: 1g solid enzyme powder (or 1ml liquid enzymes), under 70 ℃, pH=6.0 condition, in 1min, the needed enzyme amount of liquefaction 1mg Zulkovsky starch, be 1 enzyme activity unit, represents with u/g (or u/ml).In the present invention, enzyme activity unit is continued to use this definition.
According to the present invention, in step (2), mixture (or a liquefier) is not particularly limited with the weight ratio, spray regime and the injecting time that spray with steam, can be at injector known in those skilled in the art (for example, the long water heater of million smooth injectors or sky) spray contact in, preferably, the weight ratio of described the first steam and mixture is 0.05-0.1: 1, the first steam and mixture duration of contact are 1-5 second, and Contact Temperature is 80-100 ℃; The weight ratio of described the second steam and mixture is 0.05-0.1: 1, and steam and mixture duration of contact are 1-3 second, Contact Temperature is 120-150 ℃.In more preferred situation, once the timed interval of injection and second spraying is 120-180 minute.
Flash distillation of the present invention, more abundant in order to reach better the expansion that makes starch molecule that is brought by temperature variation fast, to such an extent as to can make macrobead starch swelling fracture is the purpose of starch granule, and make the better effects if that contacts of amylase and starch granules, to reach better hydrolysis result.Under preferable case, the temperature of flash distillation is 100-103 ℃, and after flash distillation, cooling is 5-10 ℃, the pressure of flash distillation is-0.06～-0.09MPa.In more preferred situation, under preferable case, the steam that obtains after flash distillation is returned in step (2) be used as the first steam; The water of condensation that obtains after flash distillation is returned in step (1) for the preparation of farinaceous size.Like this can Resource Recycling Utilization, reduce production costs.
The present invention preferably in step (4) before, the product after the flash distillation that step (3) is obtained and cooling carries out press filtration, the liquid that press filtration is obtained mixes with second section amylase.Press filtration can make the crude oil that is attached on filter residue leach under pressure thereupon, and the promotion crude oil separates with liquid glucose, thereby improves the crude oil yield; Owing to there is no filter residue in the liquefaction clear liquid, grease easily separates simultaneously; In addition, the subsequent sedimentation disengaging time shortens, and enhances productivity.Under preferable case, the pressure of described press filtration is 0.1-0.6MPa.
According to the present invention, described starchy material can be the various raw materials that contain starch that can be used for enzymolysis, fermentation well known in the art, for example, can be selected from least a in corn, potato class (as cassava), wheat and Chinese sorghum.
The present invention also provides a kind of preparation method of citric acid, and the method comprises according to aforesaid method to be processed starchy material, obtains enzymolysis product, and this enzymolysis product is fermented under aspergillus niger exists.Under preferable case, as fermented liquid, sterilization is 15-30 minute under 80-90 ℃, after being cooled to 36-38 ℃, aspergillus niger is seeded in fermented liquid, ferments with described enzymolysis product.
Because the preparation method of citric acid provided by the invention relates generally to improvement to the treatment process of the front starchy material that ferments, therefore there is no particular limitation to the process of fermentation, can be same as the prior art.For example, the condition of the kind of aspergillus niger and consumption and fermentation can be carried out with reference to prior art, and the present invention no longer repeats at this.
The present invention is further illustrated for the following examples.in following examples, in enzymolysis solid phase residue, the measuring method of residual starch content is: the enzymolysis residue of getting two parts of equivalent, portion adds amylase and water (enzymolysis residue), portion adds the water (dissolved residue) with the gross weight equivalent of amylase and water, detect respectively the content A % by weight of dissolving total reducing sugar in dissolved residue with the Fehling method, the diastatic total sugar content B % by weight that adds, the content C % by weight of enzymolysis total reducing sugar in the enzymolysis residue, detect again contents on dry basis W % by weight in the enzymolysis residue, the residual starch content of butt=(C-B-A) * 0.9/W in the enzymolysis residue.
The measuring method of the DE value of enzymolysis clear liquid is: the Fehling method is measured enzymolysis solution reducing sugar A1 (m/m), and the oven for drying method is measured enzymolysis solution dry matter content A2 (m/m), enzymolysis solution DE value=(A1/A2) * 100%.
Concentration (abbreviation acidity) according to GB 1987-2007 standard detection fermentation secondary fermentation liquid, and the transformation efficiency of calculating citric acid, the weight of the volume/total reducing sugar of the concentration of transformation efficiency (%)=fermented liquid (abbreviation acidity) * fermented liquid (total reducing sugar=seeding tank total reducing sugar+fermentor tank total reducing sugar) * 100%.
In the embodiment of the present invention, amylase used is the high temperature resistant α-amylase available from Novozymes Company; Aspergillus niger T01 used is available from Tianjin industrial microorganism institute; Injector used is million smooth injectors.
Embodiment 1
100 weight part corns are pulverized, obtained average particle diameter and be the crushed products of 400 microns, with product and 400 weight parts waters after pulverizing, mixing, obtain farinaceous size, and the pH value of this farinaceous size be adjusted to 5.5 under 45 ℃.
Under 45 ℃, the α-amylase that farinaceous size and consumption are 6.4 enzyme activity units/g Semen Maydis powder was mixed 60 minutes, obtain mixture, this mixture and the steam of 156 ℃ are sprayed in injector contact (weight ratio of steam and mixture is 0.05: 1), the time of contact is 5 seconds, making the temperature with mixture after steam contacts is 95 ℃, and laminar flow kept 120 minutes, obtains liquefier one time.
An above-mentioned liquefier is sprayed in injector with the steam of 156 ℃ again contact (weight ratio of steam and mixture is 0.05: 1), the time of contact is 3 seconds, making again the temperature with mixture after steam contacts is 120 ℃, and keeps 5 minutes at this temperature;
Again carry out flash distillation (pressure is-0.07MPa, and the time is 10 seconds) with mixture after steam contacts and to 100 ℃, be cooled to 90 ℃ above-mentioned, obtain liquefier.And the steam that obtains after flash distillation is returned in above-mentioned injecting step for the first time, water of condensation is returned to step for above-mentioned modulation farinaceous size.
Under 85 ℃, the α-amylase that this liquefier and consumption are 1.6 enzyme activity units/g Semen Maydis powder was mixed 150 minutes, obtain mixture.With this mixture under 80 ℃ standing 5 hours, isolate enzymolysis solution and enzymolysis solid phase residue, liquid collecting is entered in the clear liquid tank.The DE value of measuring enzymolysis solution is as shown in table 1.
With liquid glucose obtained above, as fermented liquid, be heated to 80 ℃ of sterilizations, keep after 30 minutes fast cooling to 36 ℃, the access aspergillus niger strain ferments, and the total reducing sugar in the detection fermented liquid, inoculum size is: 4 * 105 colony-forming units of every gram fermented liquid inoculation, and at 40 ℃, 0.2 volume: cultivated 55 hours fermentation ends under the condition of the ventilation of (volume minute).Calculate acidity and the transformation efficiency of the fermented liquid after fermenting, result is as shown in table 1.
Embodiment 2
100 weight part corns are pulverized, obtained average particle diameter and be the crushed products of 400 microns, with product and 180 weight parts waters after pulverizing, mixing, obtain farinaceous size, and the pH value of this farinaceous size be adjusted to 6.2 under 60 ℃.
Under 60 ℃, the α-amylase that farinaceous size and consumption are 12 enzyme activity units/g Semen Maydis powder was mixed 60 minutes, obtain mixture, this mixture and the steam of 160 ℃ are sprayed in injector contact (weight ratio of steam and mixture is 0.1: 1), the time of contact is 1 second, making the temperature with mixture after steam contacts is 98 ℃, and laminar flow kept 180 minutes, obtains liquefier one time.
An above-mentioned liquefier is sprayed in injector with the steam of 160 ℃ again contact (weight ratio of steam and mixture is 0.1: 1), the time of contact is 3 seconds, making again the temperature with mixture after steam contacts is 125 ℃, and keeps 5 minutes at this temperature;
Again carry out flash distillation (vacuum tightness is-0.09MPa, and the time is 10 seconds) with mixture after steam contacts and to 103 ℃, be cooled to 99 ℃ above-mentioned, obtain liquefier.
Under 99 ℃, the α-amylase that this liquefier and consumption are 12 enzyme activity units/g Semen Maydis powder was mixed 100 minutes, obtain mixture.This mixture is carried out press filtration under 95 ℃, use chamber-type press filter, stop charging when pressure reaches 0.5MPa, isolate enzymolysis solution and enzymolysis solid phase residue, liquid collecting is entered in the clear liquid tank.The DE value of measuring enzymolysis solution is as shown in table 1.
With liquid glucose obtained above, as fermented liquid, be heated to 85 ℃ of sterilizations, keep after 20 minutes fast cooling to 37 ℃, the access aspergillus niger strain ferments, and the total reducing sugar in the detection fermented liquid, inoculum size is: 4 * 105 colony-forming units of every gram fermented liquid inoculation, and at 40 ℃, 0.2 volume: cultivated 58 hours fermentation ends under the condition of the ventilation of (volume minute).Calculate acidity and the transformation efficiency of the fermented liquid after fermenting, result is as shown in table 1.
Comparative Examples 1
According to the method in embodiment 2, different is, only carry out once enzyme-added, concrete operations are as follows: 100 weight part corns are pulverized, obtain average particle diameter and be the crushed products of 400 microns, with product and 180 weight parts waters after pulverizing, mixing under 60 ℃, obtain farinaceous size, and the pH value of this farinaceous size is adjusted to 6.2.
Under 60 ℃, the α-amylase that farinaceous size and consumption are 24 enzyme activity units/g Semen Maydis powder was mixed 30 minutes, obtain mixture, this mixture and the steam of 160 ℃ are sprayed in injector contact (weight ratio of steam and mixture is 0.1: 1), the time of contact is 1 second, making the temperature with mixture after steam contacts is 98 ℃, and laminar flow kept 180 minutes, obtains liquefier one time.
An above-mentioned liquefier is sprayed in injector with the steam of 160 ℃ again contact (weight ratio of steam and mixture is 0.1: 1), the time of contact is 3 seconds, making again the temperature with mixture after steam contacts is 125 ℃, and keeps 5 minutes at this temperature;
Again carry out flash distillation (vacuum tightness is-0.09MPa, and the time is 10 seconds) with mixture after steam contacts and to 103 ℃, be cooled to 99 ℃ above-mentioned, obtain liquefier.This liquefier is carried out press filtration under 95 ℃, use chamber-type press filter, stop charging when pressure reaches 0.5MPa, isolate enzymolysis solution and enzymolysis solid phase residue, liquid collecting is entered in the clear liquid tank.
The DE value of measuring enzymolysis solution is as shown in table 1.
With liquid glucose obtained above, as fermented liquid, be heated to 85 ℃ of sterilizations, keep after 20 minutes fast cooling to 37 ℃, the access aspergillus niger strain ferments, and the total reducing sugar in the detection fermented liquid, inoculum size is: 4 * 105 colony-forming units of every gram fermented liquid inoculation, and at 40 ℃, 0.2 volume: cultivated 70 hours fermentation ends under the condition of the ventilation of (volume minute).Calculate acidity and the transformation efficiency of the fermented liquid after fermenting, result is as shown in table 1.
Embodiment 3
100 weight part corns are pulverized, obtained average particle diameter and be the crushed products of 400 microns, with product and 300 weight parts waters after pulverizing, mixing, obtain farinaceous size, and the pH value of this farinaceous size be adjusted to 5.6 under 50 ℃.
Under 50 ℃, the α-amylase that farinaceous size and consumption are 8 enzyme activity units/g Semen Maydis powder was mixed 60 minutes, obtain mixture, this mixture and the steam of 100 ℃ are sprayed in injector contact (weight ratio of steam and mixture is 0.08: 1), the time of contact is 3 seconds, making the temperature with mixture after steam contacts is 92 ℃, and laminar flow was kept 150 minutes, obtained liquefier one time.
An above-mentioned liquefier is sprayed in injector with the steam of 160 ℃ again contact (weight ratio of steam and mixture is 0.08: 1), the time of contact is 2 seconds, making again the temperature with mixture after steam contacts is 130 ℃, and keeps 5 minutes at this temperature;
Again carry out flash distillation (vacuum tightness is-0.06MPa, and the time is 10 seconds) with mixture after steam contacts and to 102 ℃, be cooled to 98 ℃ above-mentioned, obtain liquefier.
Under 98 ℃, the α-amylase that this liquefier and consumption are 12 enzyme activity units/g Semen Maydis powder was mixed 60 minutes, obtain mixture.This mixture is carried out press filtration under 95 ℃, use chamber-type press filter, stop charging when pressure reaches 0.5MPa, isolate enzymolysis solution and enzymolysis solid phase residue, liquid collecting is entered in the clear liquid tank.The DE value of measuring enzymolysis solution is as shown in table 1.
With liquid glucose obtained above, as fermented liquid, be heated to 90 ℃ of sterilizations, keep after 15 minutes fast cooling to 38 ℃, the access aspergillus niger strain ferments, and the total reducing sugar in the detection fermented liquid, inoculum size is: 4 * 105 colony-forming units of every gram fermented liquid inoculation, and at 40 ℃, 0.2 volume: cultivated 50 hours fermentation ends under the condition of the ventilation of (volume minute).Calculate acidity and the transformation efficiency of the fermented liquid after fermenting, result is as shown in table 1.
Comparative Examples 2
According to the method in embodiment 3, different is, only once spray, being about to 100 weight part corns pulverizes, obtain average particle diameter and be the crushed products of 400 microns, with product and 300 weight parts waters after pulverizing, mixing under 50 ℃, obtain farinaceous size, and the pH value of this farinaceous size is adjusted to 5.6.
Under 50 ℃, the α-amylase that farinaceous size and consumption are 8 enzyme activity units/g Semen Maydis powder was mixed 100 minutes, obtain mixture, this mixture and the steam of 158 ℃ are sprayed in injector contact (weight ratio of steam and mixture is 0.08: 1), the time of contact is 3 seconds, making the temperature with mixture after steam contacts is 95 ℃, and laminar flow was kept 180 minutes, obtained liquefier one time.
An above-mentioned liquefier is carried out flash distillation (vacuum tightness is-0.06MPa that the time is 10 seconds) to 102 ℃, be cooled to 98 ℃, obtain liquefier.
This liquefier is carried out press filtration under 95 ℃, use chamber-type press filter, stop charging when pressure reaches 0.45MPa, isolate enzymolysis solution and enzymolysis solid phase residue, liquid collecting is entered in the clear liquid tank.The DE value of measuring enzymolysis solution is as shown in table 1.
With liquid glucose obtained above, as fermented liquid, be heated to 90 ℃ of sterilizations, keep after 15 minutes fast cooling to 38 ℃, the access aspergillus niger strain ferments, and the total reducing sugar in the detection fermented liquid, inoculum size is: 4 * 105 colony-forming units of every gram fermented liquid inoculation, and at 40 ℃, 0.2 volume: cultivated 68 hours fermentation ends under the condition of the ventilation of (volume minute).Calculate acidity and the transformation efficiency of the fermented liquid after fermenting, result is as shown in table 1.
Embodiment 4
According to the method in embodiment 1, different is, the pH value of farinaceous size is adjusted to 7.0; Fermented 62 hours.Measure the DE value of enzymolysis solution; Calculate acidity and the transformation efficiency of the fermented liquid after fermenting, result is as shown in table 1.
Table 1
Data from above embodiment and table 1 can be found out, adopt the starchy material of method processing of the present invention, have guaranteed that the residual starch content in enzymolysis solid phase residue is low, and the DE value of enzymolysis solution is low, thereby has shortened the time of follow-up fermentation.
Comparative Examples 1 adopts once enzyme-added, records the DE value higher, reach same fermentation level, and the time of fermentation is longer.
Comparative Examples 2 adopts secondary enzyme-added, once sprays, although the DE value descends to some extent than Comparative Examples 1, in enzymolysis solid phase residue, residual starch content is higher.
Embodiment 4 is adjusted to 7.0 with the pH value of farinaceous size, although fermentation time also reduces to some extent, the minimizing amplitude is less; Embodiment 2 is adjusted to 6.2 with the pH value of farinaceous size, and it is larger that fermentation time reduces amplitude, illustrates the pH value is controlled in preferable range of the present invention better effects if.
Claims (12)
1. the treatment process of a starchy material, is characterized in that, the method comprises the steps:
(1) starchy material powder and water are mixed to get farinaceous size, farinaceous size is mixed with first part amylase, obtain mixture;
(2) mixture that obtains in step (1) is once sprayed under 80-100 ℃ with the first steam, obtain liquefier one time, then a liquefier is carried out second spraying with the second steam under 120-150 ℃, obtain the secondary liquefier;
(3) the secondary liquefier that obtains in step (2) is carried out flash distillation and cooling;
(4) under the amylorrhexis condition, the product after the flash distillation that step (3) is obtained and cooling mixes with second section amylase, carries out enzymolysis,
Wherein, the diastatic consumption of described first part is the 20-50 % by weight of diastatic total consumption, and the diastatic consumption of described second section is the 50-80 % by weight of diastatic total consumption.
2. method according to claim 1, wherein, in step (1), the condition that described starchy material powder mixes with water comprises: temperature is 45-60 ℃, and the pH value is controlled at 5.5-6.2, the weight ratio of starchy material powder and water is 1:1.8-4.
3. method according to claim 1, wherein, in step (1), the condition that farinaceous size mixes with first part amylase comprises: temperature is 45-60 ℃, and the time is 30-60 minute.
4. method according to claim 1, wherein, in step (4), described enzymatic hydrolysis condition comprises: hydrolysis temperature is 85-99 ℃, enzymolysis time is 60-150 minute.
5. method according to claim 1, wherein, with the dry weight basis of every gram starchy material powder, described diastatic total consumption is the 8-24 enzyme activity unit.
6. method according to claim 1, wherein, the weight ratio of described the first steam and mixture is 0.05-0.1:1, and the first steam and mixture duration of contact are 1-5 second, and Contact Temperature is 100-110 ℃; The weight ratio of described the second steam and a liquefier is 0.05-0.1:1, and be 1-3 second the duration of contact of steam and a liquefier, and Contact Temperature is 130-140 ℃.
7. according to claim 1 or 6 described methods wherein, are once sprayed and the timed interval of second spraying is 120-180 minute.
8. method according to claim 1, wherein, the temperature of flash distillation is 100-103 ℃, and after flash distillation, cooling is 5-10 ℃, and the pressure of flash distillation is-0.06～-0.09MPa.
9. according to claim 1 or 8 described methods, wherein, the method comprises also that the steam that will obtain after flash distillation returns in step (2) and is used as the first steam; The water of condensation that obtains after flash distillation is returned in step (1) for the preparation of farinaceous size.
10. according to claim 1,2 or 5 described methods, wherein, described starchy material is selected from least a in corn, potato class, wheat and Chinese sorghum.
11. the preparation method of a citric acid, the method comprise that according to claim 1-10, the described method of any one is processed starchy material, obtain enzymolysis product, and this enzymolysis product is fermented under aspergillus niger exists.
12. method according to claim 11, wherein, as fermented liquid, sterilization is 15-30 minute under 80-90 ℃, after being cooled to 36-38 ℃, aspergillus niger is seeded in fermented liquid, ferments with described enzymolysis product.
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| CN102796787B (en) * | 2012-09-07 | 2013-12-18 | 吉林中粮生化科技有限公司 | Preparation method of corn starch sugar |
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| CN103146783B (en) * | 2013-03-28 | 2015-04-22 | 中粮生物化学（安徽）股份有限公司 | Pretreatment method of starchy material |
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| CN101787382A (en) * | 2010-01-19 | 2010-07-28 | 广州双桥股份有限公司 | Method for recovering coin protein sugar dregs and preparing protein nitrogen sources and nitrogen-containing syrup |
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