CN105925642A - Method of industrial production for trehalose by way of microbial fermentation - Google Patents
Method of industrial production for trehalose by way of microbial fermentation Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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Abstract
A method of industrial production for trehalose by way of microbial fermentation comprises the steps of fermentation of trehalose hydrolase and trehalose synthase, starch liquefaction, catalytic reaction, glycosylation, decolorization and filtration, ion exchange, concentration, crystallization, centrifugation, drying, and membrane separation. The method of industrial production for trehalose by way of microbial fermentation has the following advantages: 1, starch is used as the raw material in the method to produce the trehalose through enzymatic catalysis, allowing mass and cheap industrial production of the trehalose; 2, high activity and low dosage of enzymes cause reduction of the production cost; 3, a centrifuged trehalose mother liquor is subjected to membrane separation such that the content of the trehalose is increased, and blending crystallization may be implemented to further increase the yield of the trehalose; 4, an innovative trehalose crystallization process causes the content of the trehalose to be above 50%, the content of glucose to be below 20%, and the content of the one-time crystallization product of the trehalose to be above 98.5%; 5, compared with a process of transforming the trehalose by using maltose, the method has great advantages, such as higher yield and lower production cost.
Description
Technical field
The invention belongs to starch sugar production technical field, a kind of method being specifically related to microbe fermentation method industrialized production trehalose.
Background technology
In November, 2000 FAO (Food and Agriculture Organization of the United Nation) and World Health Organization (WHO) (FAO/WHO) food additive joint specialist committee (JECFA) confirm trehalose as texturizer, stabilizer, wetting agent, the function of sweeting agent, the acceptable daily intake of trehalose is not restricted；In March, 2005, China's Ministry of Public Health approval trehalose is new forms of energy food.Trehalose produces the exploration project becoming popular, and its production method causes the extensive concern of domestic and international starch sugar manufacturing enterprise.Report has multiple production method, it is possible to industrialized production, the method for low-cost production is the most little.
Trehalose is freezing; in the environment of dry, hyperosmosis etc. are harsh, organism film, memebrane protein, DNA etc. are played protection effect; preservation and regeneration with life have the deepest relation; it it is the natural materials gazed at of pole; it is now widely used in food, medicine, cosmetic field; later along with the applied research of people's function exclusive to trehalose Yu characteristic; the exploitation of its purposes will be to more deep and broad development; application will more extend; to be that in starchy carbohydrate, consumption is bigger, the sugar products of new generation that application prospect is the most wide.
The general preparation method (microorganism extraction process, microbe fermentation method, enzyme process manufacture, method of gene recombination) of the trehalose having been reported that at present is all small test or theory, it is impossible to enough carry out industrialized production, and cost is the highest.Also having the industrialized preparing process that a kind of maltose converts, but yield is low, production cost is high.
Summary of the invention
A kind of method that present invention aim at providing microbe fermentation method industrialized production trehalose.
The purpose of the present invention is achieved through the following technical solutions:
By the method for microbe fermentation method industrialized production trehalose, comprise the steps:
(1) hydrolysis of trehalose enzyme and trehalose generate the fermentation of enzyme (X/Y): by the gene constructed recombination bacillus coli strain obtained, and use LB culture medium to cultivate Host Strains, including two steps:
1. the activation of seed and preparation: be connected in LB culture medium by above-mentioned recombination bacillus coli strain, cultivates 12h for 37 DEG C, it is thus achieved that shake-flask seed liquid；Described LB culture medium includes: bacteriological peptone 10g/L, yeast powder 5g/L, Nacl10g/L；Regulation pH value to 7.4,121 DEG C of sterilizing 20min；
2. tank fermentation is gone up: be seeded to by shake-flask seed liquid in the fermentation tank equipped with TB culture medium, cultivate the growing state to OD(antibacterial for 37 DEG C) grow to 0.6, add derivant lactose, make lactose concn reach 4mg/ml, then at 32 DEG C of inducing culture 24 36h, it is thus achieved that containing the fermentation liquid of X/Y enzyme；Twice high pressure homogenizer of fermentation liquor breaks cell wall, and then barrel filling cold preservation is standby；Described TB culture medium is made up of peptone 12g, yeast extract 24g, glycerol 4ml, autoclaving after the dissolving of each component, is cooled to 60 DEG C, then adds potassium dihydrogen phosphate and the solution of 0.72mol/L dipotassium hydrogen phosphate of the 170mol/L of 100ml sterilizing；
(2) starch liquefacation: join starch slurry concentration 14--15Be, pH value is transferred to 5.0 6.0, adds fire resistant alpha-diastase 400ml/ ton starch；Injection temperation 105 108 DEG C, is incubated 20 40 minutes, after iodine examination reaction is qualified, then secondary injection enzyme-removal temperature 130 135 DEG C, produce into liquefier；Cool to 44 46 DEG C by plate type heat exchanger, enter saccharifying tank catalysis, saccharifying；
(3) catalytic reaction: mix up liquefier temperature 44 46 DEG C in saccharifying tank, every cube of liquefier adds pullulanase 200ml, and trehalose generates enzyme Y enzyme 7000ml(enzyme fermentation liquid), hydrolysis of trehalose enzyme 2000ml (enzyme fermentation liquid)；Insulation catalytic reaction 30--36 hour, generates sugar liquid；
(4) saccharification react: the sugar liquid after catalytic reaction is warmed up to 62 DEG C, adjusts every cube of sugar liquid of PH4.2 4.5. to add compounded saccharifying enzyme 250ml. insulation saccharifying 24 hours with hydrochloric acid, after detection dextrin reaction is qualified, is warmed up to 80--85 DEG C of enzyme denaturing, generates saccharified liquid；If dextrin reaction is defective, add saccharifying enzyme, extend saccharificatinn period until qualified rear enzyme denaturing；
(5) decolorization filtering: the saccharified liquid after enzyme denaturing delivers to bleacher, by the 3% of saccharified liquid containing granular type thing adding activated carbon, 2% adding pearlite filtering aid, decolouring stirring 30 minutes, limpid, transparent, the foreign of refluxing delivers to basin before ion exchange；
(6)----positive--anion-exchange column of the moon, first returns stream, controls flow well, and electrical conductivity is less than 20us/cm, PH and is more than 4.5, delivers to concentrate front tank in ion exchange: the saccharified liquid filtered passes sequentially through sun regenerate；
(7) concentrate: the saccharified liquid quadruple effect concentrator that ion exchanges is concentrated to concentration 72% 73%, thicken tank before delivering to crystallization, produce into concentrated sugar；
(8) crystallization: concentrated sugar is lowered the temperature into crystallizer by plate-type heat-exchange, and temperature controls 46 50 DEG C, adds dry crystal seed by the 3% of gross weight, growing the grain 48 hours, temperature lowering curve controls to drop to 16 DEG C from 46 DEG C through 60 hours, and 46 30 DEG C, high-temperature region temperature slows down, and low-temperature space 30 16 DEG C fall is fast；
(9) centrifugal: to use upper suspension mechanical discharging automatic centrifuge, the sweet cake impeller pump of advantages of good crystallization delivers to distributing trough on centrifuge, open centrifuge, 5% affination of sweet cake amount is pressed with the temperature distilled water less than 20 DEG C, detection content of trehalose is more than 98.5%, if content is the most again with above way affination the most once, until reaching qualified；
(10) being dried: being dried with boiling fluidised-bed drying machine or pneumatic conveying drying unit, one time hot blast temperature controls to 65 75 DEG C, and secondary hot-air temperature controls to 75 85 DEG C, cold wind temperature is less than 20 DEG C, and commerieal sugar temperature is less than 37 DEG C of packagings；
(11) membrance separation: the mother solution after Li Xin contains glucose 21%, containing trehalose 20%, uses the organic molecular film of particular bore warp, is separated with trehalose by the glucose in mother solution；Content of trehalose can return more than 80% and be fitted on condensing crystallizing in ion exchange liquid, and glucose content can be used for more than 95% producing high fructose syrup.
Beneficial effects of the present invention: 1, the present invention is with starch as raw material, enzyme law catalysis produces trehalose, enables that trehalose is substantial amounts of, carry out industry law production at a low price, is also the promotion the biggest to starch industry and development.2, enzyme activity is high, and consumption is few, and can be used directly to catalytic production with fermentation liquid, reduces production cost.3, centrifugal trehalose mother solution is improved by membrance separation, content of trehalose, can be returned and join crystallization, improves trehalose yield further.4, the trehalose crystallization processes of innovation, by the saccharified liquid after catalytic reaction, by refining steps such as decolouring, ion exchanges, content of trehalose is more than 50%, glucose content is below 20%, by arranging the important indicators such as rational cerium sulphate crystal index, temperature lowering curve, sugar entering saturation, sugar entering temperature, primary crystallization trehalose finished product content is up to more than 98.5%.5 have great advantage with compared with maltose conversion Technology of Trehalose, and yield is high, and production cost is low.
Detailed description of the invention
The following examples can illustrate in greater detail the present invention, but limits the present invention the most in any form.
Embodiment 1:
Weighing corn starch 1200kg, add water stirring regulate pH value 5.68, starch slurry concentration 14Be, injection temperation 106 DEG C, be incubated 40 minutes, and inspection iodine colour response is qualified, secondary injection temperature 135 DEG C；Obtaining liquefier 4.06M, concentration 23.6Bx, liquefy DE value 9.83%；Cool to 46 DEG C, add pullulanase 800ml, generate enzyme Y enzyme 28kg(enzyme fermentation liquid with trehalose), hydrolysis of trehalose enzyme Z enzyme 8kg(enzyme fermentation liquid), catalytic reaction 30h；It is warmed up to 61 DEG C, adjusts PH4.4 with hydrochloric acid, add saccharifying enzyme 400ml, saccharification react 24h, check that dextrin reaction is qualified；It is warmed up to 85 DEG C of enzyme denaturing and is incubated 30 minutes, add activated carbon decolorizing and filter, obtain concentration 23.5%, electrical conductivity 1365us/cm, the decolouring clear liquid of volume 3.97M；By cation and anion exchange, electrical conductivity drops to 21us/cm, PH5.8；It is concentrated to concentration 72.2%, volume 1.03M, content of trehalose 71.22%, glucose content 28.10%；In 1.2M crystallizer, adding crystal seed 3kg, temperature drops to 16 DEG C from 48 DEG C；It is centrifuged and obtains tide sugar 480kg.Being dried with boiling fluidised-bed drying machine or pneumatic conveying drying unit, one time hot blast temperature controls to 65 75 DEG C, and secondary hot-air temperature controls to 75 85 DEG C, and cold wind temperature is less than 20 DEG C, and commerieal sugar temperature is less than 37 DEG C of packagings；The trehalose mother solution that will be centrifuged out, after membrance separation, a kind of containing glucose 95.5%, production results glucose is starched, another kind of containing trehalose 78.5%, to be fitted on for sugared time concentration with front workshop section ion exchange and crystallize again, concentration 72.5%, volume 1.10M, content of trehalose 75.12%, glucose content 24.21%, in 1.2M crystallizer, add crystal seed 3kg, temperature drops to 16 degree from 48 degree, is centrifuged and obtains tide sugar 502kg.
As shown in the table by crystalline trehalose GB GB/T23529 2009 testing result:
The explanation of above example is only intended to help to understand method and the core concept thereof of the present invention.It should be pointed out that, for those skilled in the art, under the premise without departing from the principles of the invention, it is also possible to the present invention is carried out some improvement and modification, these improve and modify in the protection domain also falling into the claims in the present invention.
Claims (1)
1. by the method for microbe fermentation method industrialized production trehalose, it is characterised in that comprise the steps:
(1) hydrolysis of trehalose enzyme and trehalose generate the fermentation of enzyme (X/Y): by the gene constructed recombination bacillus coli strain obtained, and use LB culture medium to cultivate Host Strains, including two steps:
1. the activation of seed and preparation: be connected in LB culture medium by above-mentioned recombination bacillus coli strain, cultivates 12h for 37 DEG C, it is thus achieved that shake-flask seed liquid；Described LB culture medium includes: bacteriological peptone 10g/L, yeast powder 5g/L, Nacl10g/L；Regulation pH value to 7.4,121 DEG C of sterilizing 20min；
2. tank fermentation is gone up: be seeded to by shake-flask seed liquid in the fermentation tank equipped with TB culture medium, cultivate the growing state to OD(antibacterial for 37 DEG C) grow to 0.6, add derivant lactose, make lactose concn reach 4mg/ml, then at 32 DEG C of inducing culture 24 36h, it is thus achieved that containing the fermentation liquid of X/Y enzyme；Twice high pressure homogenizer of fermentation liquor breaks cell wall, and then barrel filling cold preservation is standby；Described TB culture medium is made up of peptone 12g, yeast extract 24g, glycerol 4ml, autoclaving after the dissolving of each component, is cooled to 60 DEG C, then adds potassium dihydrogen phosphate and the solution of 0.72mol/L dipotassium hydrogen phosphate of the 170mol/L of 100ml sterilizing；
(2) starch liquefacation: join starch slurry concentration 14--15Be, pH value is transferred to 5.0 6.0, adds fire resistant alpha-diastase 400ml/ ton starch；Injection temperation 105 108 DEG C, is incubated 20 40 minutes, after iodine examination reaction is qualified, then secondary injection enzyme-removal temperature 130 135 DEG C, produce into liquefier；Cool to 44 46 DEG C by plate type heat exchanger, enter saccharifying tank catalysis, saccharifying；
(3) catalytic reaction: mix up liquefier temperature 44 46 DEG C in saccharifying tank, every cube of liquefier adds pullulanase 200ml, and trehalose generates enzyme Y enzyme 7000ml(enzyme fermentation liquid), hydrolysis of trehalose enzyme 2000ml (enzyme fermentation liquid)；Insulation catalytic reaction 30--36 hour, generates sugar liquid；
(4) saccharification react: the sugar liquid after catalytic reaction is warmed up to 62 DEG C, adjusts every cube of sugar liquid of PH4.2 4.5. to add compounded saccharifying enzyme 250ml. insulation saccharifying 24 hours with hydrochloric acid, after detection dextrin reaction is qualified, is warmed up to 80--85 DEG C of enzyme denaturing, generates saccharified liquid；If dextrin reaction is defective, add saccharifying enzyme, extend saccharificatinn period until qualified rear enzyme denaturing；
(5) decolorization filtering: the saccharified liquid after enzyme denaturing delivers to bleacher, by the 3% of saccharified liquid containing granular type thing adding activated carbon, 2% adding pearlite filtering aid, decolouring stirring 30 minutes, limpid, transparent, the foreign of refluxing delivers to basin before ion exchange；
(6)----positive--anion-exchange column of the moon, first returns stream, controls flow well, and electrical conductivity is less than 20us/cm, PH and is more than 4.5, delivers to concentrate front tank in ion exchange: the saccharified liquid filtered passes sequentially through sun regenerate；
(7) concentrate: the saccharified liquid quadruple effect concentrator that ion exchanges is concentrated to concentration 72% 73%, thicken tank before delivering to crystallization, produce into concentrated sugar；
(8) crystallization: concentrated sugar is lowered the temperature into crystallizer by plate-type heat-exchange, and temperature controls 46 50 DEG C, adds dry crystal seed by the 3% of gross weight, growing the grain 48 hours, temperature lowering curve controls to drop to 16 DEG C from 46 DEG C through 60 hours, and 46 30 DEG C, high-temperature region temperature slows down, and low-temperature space 30 16 DEG C fall is fast；
(9) centrifugal: to use upper suspension mechanical discharging automatic centrifuge, the sweet cake impeller pump of advantages of good crystallization delivers to distributing trough on centrifuge, open centrifuge, 5% affination of sweet cake amount is pressed with the temperature distilled water less than 20 DEG C, detection content of trehalose is more than 98.5%, if content is the most again with above way affination the most once, until reaching qualified；
(10) being dried: being dried with boiling fluidised-bed drying machine or pneumatic conveying drying unit, one time hot blast temperature controls to 65 75 DEG C, and secondary hot-air temperature controls to 75 85 DEG C, cold wind temperature is less than 20 DEG C, and commerieal sugar temperature is less than 37 DEG C of packagings；
(11) membrance separation: the mother solution after Li Xin contains glucose 21%, containing trehalose 20%, uses the organic molecular film of particular bore warp, is separated with trehalose by the glucose in mother solution；Content of trehalose can return more than 80% and be fitted on condensing crystallizing in ion exchange liquid, and glucose content can be used for more than 95% producing high fructose syrup.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107488689A (en) * | 2017-10-10 | 2017-12-19 | 溧阳维信生物科技有限公司 | α, the method for alpha trehalose dihydrate are prepared using electrodialytic technique |
| CN107739745A (en) * | 2017-10-10 | 2018-02-27 | 溧阳维信生物科技有限公司 | α, the method for alpha trehalose dihydrate are prepared using membrane separation technique |
| CN108588148A (en) * | 2018-07-06 | 2018-09-28 | 安徽民祯生物工程有限公司 | A kind of method of rice fermentation production trehalose |
| CN108624637A (en) * | 2018-08-24 | 2018-10-09 | 湖南汇升生物科技有限公司 | A method of utilizing rice production trehalose |
| CN109251952A (en) * | 2018-09-18 | 2019-01-22 | 溧阳维信生物科技有限公司 | A method of preparing 79-87% a, a-trehalose dihydrate |
| CN110055293A (en) * | 2019-04-29 | 2019-07-26 | 长沙而道新能源科技有限公司 | A kind of synthetic method of trehalose |
| WO2019144588A1 (en) * | 2018-01-26 | 2019-08-01 | 通辽梅花生物科技有限公司 | Preparation method of large-grained trehalose |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1807465A (en) * | 2006-01-11 | 2006-07-26 | 胡耀辉 | Mycose separation and purification method |
| CN102337313A (en) * | 2011-10-17 | 2012-02-01 | 山东天力药业有限公司 | Method for preparing trehalose |
| CN102586363A (en) * | 2012-03-06 | 2012-07-18 | 禹城绿健生物技术有限公司 | Maltose production and refining method |
| CN103952453A (en) * | 2014-05-20 | 2014-07-30 | 彭燕辉 | Method for preparing trehalose |
| CN104212854A (en) * | 2014-09-22 | 2014-12-17 | 吉林省轻工业设计研究院 | Method for producing isomalto-oligosaccharide by using high-concentration starch |
| CN105039371A (en) * | 2015-07-21 | 2015-11-11 | 齐鲁工业大学 | Trehalose synthase-trehalose hydrolase fusion enzyme, expression gene thereof and application |
| CN105039191A (en) * | 2015-09-09 | 2015-11-11 | 齐鲁工业大学 | Surface display method and application of trehalose synthase and trehalose-hydrolysing |
-
2016
- 2016-06-14 CN CN201610413674.3A patent/CN105925642B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1807465A (en) * | 2006-01-11 | 2006-07-26 | 胡耀辉 | Mycose separation and purification method |
| CN102337313A (en) * | 2011-10-17 | 2012-02-01 | 山东天力药业有限公司 | Method for preparing trehalose |
| CN102586363A (en) * | 2012-03-06 | 2012-07-18 | 禹城绿健生物技术有限公司 | Maltose production and refining method |
| CN103952453A (en) * | 2014-05-20 | 2014-07-30 | 彭燕辉 | Method for preparing trehalose |
| CN104212854A (en) * | 2014-09-22 | 2014-12-17 | 吉林省轻工业设计研究院 | Method for producing isomalto-oligosaccharide by using high-concentration starch |
| CN105039371A (en) * | 2015-07-21 | 2015-11-11 | 齐鲁工业大学 | Trehalose synthase-trehalose hydrolase fusion enzyme, expression gene thereof and application |
| CN105039191A (en) * | 2015-09-09 | 2015-11-11 | 齐鲁工业大学 | Surface display method and application of trehalose synthase and trehalose-hydrolysing |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107488689A (en) * | 2017-10-10 | 2017-12-19 | 溧阳维信生物科技有限公司 | α, the method for alpha trehalose dihydrate are prepared using electrodialytic technique |
| CN107739745A (en) * | 2017-10-10 | 2018-02-27 | 溧阳维信生物科技有限公司 | α, the method for alpha trehalose dihydrate are prepared using membrane separation technique |
| WO2019144588A1 (en) * | 2018-01-26 | 2019-08-01 | 通辽梅花生物科技有限公司 | Preparation method of large-grained trehalose |
| JP2021511835A (en) * | 2018-01-26 | 2021-05-13 | トンリャオ メイファ バイオロジカル サイ−テック カンパニー リミテッドＴｏｎｇｌｉａｏ Ｍｅｉｈｕａ Ｂｉｏｌｏｇｉｃａｌ Ｓｃｉ−Ｔｅｃｈ Ｃｏ．， Ｌｔｄ | Method for producing large particle trehalose |
| JP7075506B2 (en) | 2018-01-26 | 2022-05-25 | トンリャオ メイファ バイオロジカル サイ－テック カンパニー リミテッド | Manufacturing method of large particle trehalose |
| CN108588148A (en) * | 2018-07-06 | 2018-09-28 | 安徽民祯生物工程有限公司 | A kind of method of rice fermentation production trehalose |
| CN108624637A (en) * | 2018-08-24 | 2018-10-09 | 湖南汇升生物科技有限公司 | A method of utilizing rice production trehalose |
| CN108624637B (en) * | 2018-08-24 | 2021-10-19 | 湖南汇升生物科技有限公司 | Method for producing trehalose by using rice |
| CN109251952A (en) * | 2018-09-18 | 2019-01-22 | 溧阳维信生物科技有限公司 | A method of preparing 79-87% a, a-trehalose dihydrate |
| CN110055293A (en) * | 2019-04-29 | 2019-07-26 | 长沙而道新能源科技有限公司 | A kind of synthetic method of trehalose |
| CN110055293B (en) * | 2019-04-29 | 2020-10-27 | 郑州谦益食品有限公司 | Trehalose synthesis method |
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