CN1290753A

CN1290753A - Method for preparing high-purity oligomer fructose

Info

Publication number: CN1290753A
Application number: CN 00130200
Authority: CN
Inventors: 牟云青
Original assignee: TIANYUAN HEALTH FOOD CO Ltd YUNNAN PROV
Current assignee: YUNNAN JIANSHENG BIOTECHNOLOGY Co.,Ltd.; Yunnan Kin Biotechnology Co.,Ltd.
Priority date: 2000-10-20
Filing date: 2000-10-20
Publication date: 2001-04-11
Anticipated expiration: 2020-10-20
Also published as: CN1125879C

Abstract

Through a simple technological process, effective components tri-, tetra-and penta-frustose content is raised and oligomer fractose in sucrose solution is increased. Aspergillus niger V. Tiegh strain in two-stage cultured to obtain fructore transferase, which in added into 30-50 % concentration sucrose colution in a enzymolyzing tank to produce the first enzymolyzing contact reaction at pH 5-8 and 65-70 deg.C for 8-10 hr. The product of the first enzymolysis is filtered and the filtered sucrose size is enzymoplyzed and filtered for the second time.

Description

Method for preparing high-purity oligomer fructose

The present invention relates to a kind of preparation method of novel function food.

Oligofructose (hereinafter to be referred as FOS) is that a kind of to be no more than four fructose be chain link, and is the fructooligosaccharide of the end group of chain with glucose, has another name called Nutriflora P or fructose oligosaccharides.Oligofructose is distributed widely in the foods such as various fruit, vegetables at nature, contains oligofructose 0.6% as garlic, contains 0.75% in the honey, but therefrom extraction is very difficult, the lower and very difficult realization suitability for industrialized production of yield.People such as nineteen fifty Bacon find to act on sucrose by yeast, can shift fructose and generate oligofructose.Its effective ingredient is kestose (GF ₂), GF3 (GF ₃) and GF4 (GF ₄).Japan has realized suitability for industrialized production in nineteen eighty-three, and the commercialization oligofructose is come into the market, and it is divided into G type (FOS 〉=50%) and P type (FOS 〉=90%).Because its good nourishing function and special physiologically active, oligofructose as a kind of internationally recognized typical prebiotics representative, are applied in 500 numerous food and the part medicine extremely modern food manufacturing enterprise and human consumer favor.

Present known oligofructose physiologic function mainly contains:

One, good health care effect has good physiologically active, and alternative is bred rapidly the probiotics bifidus bacillus in people's coelenteron, and can significantly suppress the growth and breeding of harmful bacterium, reaches the effect of two-ways regulation human body microecological balance;

Two, be similar to soluble dietary fibre, do not reduced the enteric cavity pH value, promote the enteric cavity wriggling, in company with the effect of bifidus bacillus, play relaxing bowel, the effect of blood fat reducing, dispelling toxicity, detoxicating by the digestion enzymolysis;

Three, effectively improve antibody forming cell's number and NK cytoactive, strengthened body immunity;

But four reducing total cholesterol and triglyceride play reducing blood lipid, and the blood glucose value that do not raise;

Five, can effectively synthesize vitamin B group, promote trace elements absorbed such as calcium, iron, zinc, have anti-dental caries, and can prevent colorectal carcinoma.

At present, the method for suitability for industrialized production FOS is divided into two kinds: first kind is to adopt the enzyme of fructosyl transfer activity to act on the method that sucrose is produced, and is called for short enzyme process.The liquid enzymes method and the immobilized enzyme method that on the enzyme reaction formation, are divided into suspension bed again.Second kind is by method of extraction in the plant witloof.The immobilized enzyme method of first method exists yield and transformation efficiency low, the GF in the oligofructose ₃And GF ₄Content is higher for total reducing sugar, and its effective physiologically active descends, and complicated process of preparation also causes the content of FOS low, owing to contain a large amount of colloid albumen or salt, and make product appearance seem muddy, and shade deviation; Product with a kind of method production in back, the polymerization degree is between 2～60, big and the sugarcane fruit three, four of molecular weight dispersity, pentasaccharides content are seldom, mostly be the high polymers of fructose, major part is digested in stomach and small intestine, can arrive large intestine on a small quantity, relatively poor to the cultivation effect of bifidus bacillus, the grease mouthfeel deficiency that is used for replenishing some food more.

The object of the present invention is to provide a kind of improved method for preparing high-purity oligomer fructose, it can further improve effective ingredient sugarcane fruit three, four, pentasaccharides in the product, the transformation efficiency of oligofructose in the sucrose solution is improved, and this preparation method's technology is simple, and cost is low.

Purpose of the present invention is achieved through the following technical solutions:

The oligofructose preparation method adopts aspergillus niger (Aspergillus nigerV.Tiegh) bacterial classification, obtain fructose-transferring enzyme through the secondary cultivation, add concentration and be in 30%～50% the sucrose solution, transferring pH value in the enzyme jar is 5～8, carry out the enzyme contact reacts under 62～70 ℃ one time, reaction was carried out 8～10 hours, and the product of an enzymeization carries out a nanofiltration to be handled, filtrate syrup after the nanofiltration carries out the secondary enzymeization, and the syrup with enzymeization carries out secondary nanofiltration processing more then.

Preparation method provided by the invention is acted on by fructose-transferring enzyme to carry out nanofiltration behind the sucrose molecules and handle and obtain product.Sucrose (GF represents with symbol) is made up of glucose (G represents with symbol) and fructose (symbol F represents), and solution forms oligofructose GF by each glucose molecule of effect of fructose-transferring enzyme in conjunction with 2～4 fructose molecules ₂, GF ₃, GF ₄, wherein a part of sucrose and glucose carry out nanofiltration again to be handled, and can obtain the high-purity fructo oligosaccharides of content 〉=90%.

The bacterial classification of selecting for use in the preparation is a kind of of aspergillus niger, belong to Aspergillus nigerV.Tiegh, bacterial classification is through optimizing screening, on aseptic condition, insert on the nutrient agar that mixes up in the eggplant bottle and cultivate, guarantee the fructose-transferring enzyme of the high vigor of enlarged culturing output step by step through secondary; In the enzyme process every processing condition are wanted strict control, guarantee that FOS purity after every batch of enzymeization is more than 55%.Can improve the physical and chemical index and the microbiological indicator of product after enzymeization is finished effectively, the product of acquisition is no matter in content, the outward appearance of working substance oligofructose with all be better than on the quality guaranteed period with immobilized enzyme method and plant extraction method.

The invention will be further described below in conjunction with embodiment and accompanying drawing thereof.

Accompanying drawing 1 is a process flow sheet of the present invention.

Fig. 2 is a nanofiltration principle of work synoptic diagram.

The preparation method: 1. adopt the bacterial classification of fructose-transferring enzyme, after microorganism was cultivated, centrifugation obtained fructose Transferase, adding concentration is 30%～50% sucrose solution, carries out once under 62～70 ℃ in the enzyme tank The enzyme haptoreaction, reaction was carried out 8～10 hours, obtained the FOS syrup of content 〉=50% after reaction is finished, Wherein accessory substance is completely sucrose of glucose and unreacted; 2. get FOS after removing accessory substance with nanofiltration equipment 〉=80%; 3. the solution of holding back after nanofiltration being processed carries out the syrup that the secondary enzyme reaction gets FOS 〉=85%, 4. will The syrup of secondary response carries out nanofiltration again and processes to get FOS 〉=90%; 5. the syrup of processing through the previous step nanofiltration advances The row decolorization filtering, spray-drying can be packed after the sterilization again.

Example one: get 1000kg one-level white sugar and add the hot water dissolving, amount of water is to 30～50% of solid content, the fructose-transferring enzyme 24kg that adds high vigor then, transfer pH value 5～8,62～70 ℃ of temperature of reaction, carry out under 8～10 hours reaction times, reaction soln is filtered decolouring, 50～60 ℃ of bleaching temperatures, bleaching time 45 minutes carries out press filtration after the decolouring, filtrate is the micropore filtering film of 0.1 μ m again through the hole, last vacuum scraper plate concentrates 70～80BAX, get final product the oligofructose syrup of 1130kg working substance content 〉=58%.

Example two: get in the example through the G of enzymeization type product 10kg, after being diluted to concentration and being 20～40% syrup with softening pure water (RO), with 5 μ m strainer millipore filtrations, with the high-pressure pump nanofiltration that circulates, solid content is not less than 8% in the nanofiltration process, keeps solid content to be advisable 10～20%.Filtrate syrup after the nanofiltration is carried out the secondary enzymeization.Syrup with the secondary enzymeization carries out secondary nanofiltration processing more then, uses the activated carbon decolorizing Plate Filtration again, and pH value maintains 5～8, last spray-dried high-purity fructo oligosaccharides pulvis, particle and three kinds of products of tablet that make 9.8kg content 〉=90% of filtrate.

Claims

1, a kind of method for preparing high-purity oligomer fructose, with sucrose is raw material, it is characterized in that: adopt aspergillus niger (Aspergillus nigerV.Tiegh) bacterial classification, obtain fructose-transferring enzyme through the secondary cultivation, add concentration and be in 30%～50% the sucrose solution, transferring pH value in the enzyme jar is 5～8, under 62～70 ℃, carry out the enzyme contact reacts one time, reaction was carried out 8～10 hours, the product of an enzymeization carries out a nanofiltration to be handled, filtrate syrup after the nanofiltration carries out the secondary enzymeization, and the syrup with enzymeization carries out secondary nanofiltration processing more then.

2, method according to claim 1 is characterized in that: can filter decolouring to solution after the enzyme reaction, and 50～60 ℃ of bleaching temperatures, bleaching time 45 minutes carries out press filtration after the decolouring, and filtrate is the micropore filtering film of 0.1 μ m again through the hole.

3, method according to claim 1 is characterized in that: the filtrate after the secondary nanofiltration is handled can be filtered with activated carbon decolorizing, and pH value maintains 5～8, and spraying drying can be packed after the sterilization again.