CN101289681A - Method of producing high-purity fructo oligosaccharides - Google Patents
Method of producing high-purity fructo oligosaccharides Download PDFInfo
- Publication number
- CN101289681A CN101289681A CNA2007100216059A CN200710021605A CN101289681A CN 101289681 A CN101289681 A CN 101289681A CN A2007100216059 A CNA2007100216059 A CN A2007100216059A CN 200710021605 A CN200710021605 A CN 200710021605A CN 101289681 A CN101289681 A CN 101289681A
- Authority
- CN
- China
- Prior art keywords
- fructo oligosaccharides
- content
- oligofructose
- oligosaccharides
- percent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for producing high purity of fructo oligosaccharides. The method comprises the steps of converting raw material of sucrose through enzymatic industrialized production to obtain ordinary grade fructo oligosaccharides with the content around 50 percent; then eliminating glucose in the ordinary grade fructo oligosaccharides by adopting the biotransformation principle and yeast transformation to make the content of the fructo oligosaccharides reach over 75 percent; and removing other composition by combining ion-exchange chromatography separation and membrane Separation technology to make the content of the fructo oligosaccharides reach over 95 percent. The method has the advantage that the production technology is reasonable and scientific, the fructo oligosaccharides with high purity can be extracted and industrial mass production can be realized.
Description
Technical field
The present invention relates to a kind of production method of health food-Yizhibao, relate in particular to a kind of production method of high-purity fructo oligosaccharides.
Background technology
Oligofructose (being called for short FOS), claim oligofructose or kestose family oligose again, be to utilize the enzyme that has the fructose transfer activity in the microorganism to act on sucrose and the product that contains kestose, GF3 and GF4 that obtains, product has good nourishing function and physiology characteristic.Concrete property has: 1, can be utilized selectively by a class bacterium (bifidus bacillus) in the human body large intestine, behind the picked-up oligofructose, make the intravital bifidus bacillus quantity of people increase by 100 to 1000 times; 2,, thereby be a kind of low-calorie sweetener not by the human body alimentary canal enzymic hydrolysis; 3, be a kind of water-soluble foodstuff fibre, the energy blood fat reducing improves lipid metabolism, and after patient took, its cholesterol, triglyceride and free fatty acids all reduced; 4, prevention of dental caries, oligofructose can not be used as the formation matrix of carious tooth, also do not have the thalline coagulation, and the lactic acid content of generation is 10 to 50% of a sucrose; 5, suppress the growth of the interior spoilage organism of intestines and other harmful bacterium, promote functions of intestines and stomach.At present domestic oligofructose that can only the production regular grade, its content be only about 55% (weight ratio), also contains have an appointment 20% glucose, fructose and sucrose by product in the product, and these by products are unsuitable to diabetics and eurodonticus, make the function reduction of oligose.The preparation method of existing high-purity fructo oligosaccharides is the patent of invention of CN00130200.0 referring to the patent No., its preparation technology mainly is to be raw material with sucrose, adopt aspergillus niger strain, obtain fructose-transferring enzyme through the secondary cultivation, join concentration and be in 30% to 50% the sucrose solution, transferring pH value in the enzyme jar is 5-8%, under 62-70 degree Celsius, carry out one time the enzyme contact reacts, reaction was carried out 8-10 hour, the product of an enzymeization carries out a nanofiltration to be handled, filtrate syrup after the nanofiltration carries out the secondary enzymeization, and the syrup with enzymeization carries out secondary nanofiltration processing more then, thereby obtains highly purified oligofructose.The oligofructose that adopts this processing method to purify is difficult to reach the purity more than 95%.
Summary of the invention
The object of the present invention is to provide and a kind ofly can effectively improve oligofructose purity, and the relatively rational production method of high-purity fructo oligosaccharides of technology.
For realizing purpose of the present invention, a kind of production method of high-purity fructo oligosaccharides, its step mainly comprises: employing sucrose is raw material, produces through the industrialization enzymatic conversion method, obtains content and be about 55% regular grade oligofructose; Then, utilize the bio-transformation principle, adopt the glucose in the yeast conversion elimination regular grade oligofructose, the content of oligofructose is reached more than 75%; Utilize ion-exchange chromatography separation and membrane separation technique to combine again, remove wherein other composition, the end levan content in the product is reached more than 95%.
The invention has the beneficial effects as follows: production technique is scientific and reasonable, can extract highly purified oligofructose, and can realize industrialized mass production.
Production technique to a kind of high purity oligose of the present invention is described in further detail below:
Embodiment
A kind of production method of high-purity fructo oligosaccharides, its concrete steps comprise: employing sucrose is raw material, produce through the industrialization enzymatic conversion method, this industrialization Production by Enzymes technology is a prior art, obtain content and be about 55% regular grade oligofructose, also contain other byproducts such as glucose in this oligofructose; Then, utilize the bio-transformation principle, adopt the glucose in the yeast conversion elimination regular grade oligofructose, the content of oligofructose is reached more than 75%; At last, utilize ion-exchange chromatography separation and membrane separation technique to combine again, remove wherein other composition, the end levan content in the product is reached more than 95%.
Claims (1)
1, a kind of production method of high-purity fructo oligosaccharides, its step mainly comprises: employing sucrose is raw material, produces through the industrialization enzymatic conversion method, obtains content and be about 55% regular grade oligofructose; Then, utilize the bio-transformation principle, adopt the glucose in the yeast conversion elimination regular grade oligofructose, the content of oligofructose is reached more than 75%; Utilize ion-exchange chromatography separation and membrane separation technique to combine again, remove wherein other composition, the end levan content in the product is reached more than 95%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100216059A CN101289681A (en) | 2007-04-17 | 2007-04-17 | Method of producing high-purity fructo oligosaccharides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100216059A CN101289681A (en) | 2007-04-17 | 2007-04-17 | Method of producing high-purity fructo oligosaccharides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101289681A true CN101289681A (en) | 2008-10-22 |
Family
ID=40034126
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100216059A Pending CN101289681A (en) | 2007-04-17 | 2007-04-17 | Method of producing high-purity fructo oligosaccharides |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101289681A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102618599A (en) * | 2011-01-28 | 2012-08-01 | 上海赛奥分离技术工程有限公司 | Multistage membrane separation purification method of high-purity fructo-oligosaccharide |
| CN104171800A (en) * | 2014-08-08 | 2014-12-03 | 山东百龙创园生物科技有限公司 | Isomaltooligosacharide-fructooligosaccharide composite particle and preparation method thereof |
| CN111466578A (en) * | 2020-03-26 | 2020-07-31 | 上海儿童营养中心有限公司海南分公司 | Fructooligosaccharide prebiotics powder and production method thereof |
-
2007
- 2007-04-17 CN CNA2007100216059A patent/CN101289681A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102618599A (en) * | 2011-01-28 | 2012-08-01 | 上海赛奥分离技术工程有限公司 | Multistage membrane separation purification method of high-purity fructo-oligosaccharide |
| CN102618599B (en) * | 2011-01-28 | 2014-02-05 | 上海赛奥分离技术工程有限公司 | Multistage membrane separation purification method of high-purity fructo-oligosaccharide |
| CN104171800A (en) * | 2014-08-08 | 2014-12-03 | 山东百龙创园生物科技有限公司 | Isomaltooligosacharide-fructooligosaccharide composite particle and preparation method thereof |
| CN111466578A (en) * | 2020-03-26 | 2020-07-31 | 上海儿童营养中心有限公司海南分公司 | Fructooligosaccharide prebiotics powder and production method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101540230B1 (en) | BRANCHED α-GLUCAN, α-GLUCOSYLTRANSFERASE PRODUCING THE SAME, METHOD FOR PRODUCING THE SAME AND USE THEREOF | |
| CN102471792B (en) | Process for the production of ultrapure galacto-oligosaccharides | |
| Marim et al. | Xylooligosaccharides: prebiotic potential from agro-industrial residue, production strategies and prospects | |
| CN102127574A (en) | Method for producing fructooligosaccharide by using transfructosylase | |
| KR101458111B1 (en) | Method for Manufacturing Herb Candy Using Isomalto-oligosaccharide Syrup Produced from Organic Rice | |
| CN101591689B (en) | Method for producing isomaltulose by transforming sucrose by biological enzymatic method | |
| KR101446489B1 (en) | Method for Manufacturing High Purity Isomalto-oligosaccharide Syrup Using Organic Rice | |
| CN101200750B (en) | Rhubarb horsetails Erwinia sp. and its application in preparation of isomaltulose | |
| CN109439552A (en) | One Aspergillus oryzae BLCY-006 and its preparing the application in galactooligosaccharide | |
| JPS5953834B2 (en) | Bifidobacterium growth promoter | |
| JP3553866B2 (en) | Composition based on mannooligosaccharides | |
| CN101289681A (en) | Method of producing high-purity fructo oligosaccharides | |
| CN114134189B (en) | Method for synchronously producing low-calorie syrup containing trehalose and erythritol | |
| JP2806522B2 (en) | Method for producing branched fructooligosaccharide | |
| CN108013306A (en) | The preparation method of juice drinks rich in psicose | |
| CN1125879C (en) | Method for preparing high-purity oligomer fructose | |
| CN1231593C (en) | Technology for producing glucomannan using neutral beta-mannase to degradate fine konjaku flour | |
| JP2004159659A (en) | Composition mainly composed of mannooligosaccharide | |
| KR101105391B1 (en) | Food composition comprising enzymatic hydrolysates of kudzu root and preparation method thereof | |
| CN106755197B (en) | Method for preparing linear maltohexaose by using linear maltooligosaccharide generating enzyme | |
| Cheng et al. | Difructose anhydride III: A 50-year perspective on its production and physiological functions | |
| US20060178340A1 (en) | Composition comprising soluble glucan oligomer from saccharomyces cerevisiae is2 for immune activation or prevention and treatment of cancer and the preparation method thereof | |
| CN107287258A (en) | A kind of preparation method of the resistant dextrin containing oligoisomaltose | |
| CN106148450B (en) | High-yield zinc-rich biological cellulose gel product | |
| Hassan et al. | Lactose Derivatives: Properties, Preparation and Their Applications in Food and Pharmaceutical Industries |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20081022 |