CN100999767A - Tech, process of producing high pureness oligoiso maltose by yeast - Google Patents
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Abstract
The technological process of producing high purity isomaltose oligomer with distillers' yeast includes the steps of making milk with corn starch, liquefying, saccharifying, converting into glycoside, hydrolysis and purification with domesticated and cultured distillers' yeast, filtering, decolorizing, refining, and concentrating to obtain high purity isomaltose oligomer syrup. The isomaltose oligomer syrup may be further spray dried to obtain high purity isomaltose oligomer powder. The present invention produces high purity isomaltose oligomer by means of distillers' yeast containing several kinds of enzyme and with excellent hydrolysis effect and fermentation effect. The technological process may be completed in available apparatus for producing type-500 maltose oligomer, has low production cost and simple production process, and is suitable for industrial production.
Description
Technical field the invention belongs to the Dian Fentang technical field, specifically a kind of processing method of utilizing distiller's yeast production high purity (900 type) oligomeric isomaltose.
Background technology is present, and the method for some enterprise's production high purity (900 type) oligomeric isomaltose is to adopt the nanofiltration separation method.This production method need be invested expensive separating device, and the production cost height.
A kind of research of separation and purification oligomeric isomaltose was also disclosed on the 27th page of magazine " foodstuffs industry science and technology " the 5th phase of publishing in 2002, this research is to utilize yeast fermentation to have selectivity, be its can ferment glucose and maltose in the commodity oligomeric isomaltose, and unfermentable wherein branch oligose such as isomaltose, panose, Isomaltotriose, thereby reach the purpose that improves oligomeric isomaltose purity.Though this production method also is to utilize fermentative Production high purity oligomeric isomaltose, but the required yeast saccharomyces cerevisiae of this production method costs an arm and a leg, and consumes greatly, and needs the special fermentation equipment of investment, needed saccharification simultaneously, transglucosidase preparation consumption are big, its production cost height.
Summary of the invention the object of the present invention is to provide and a kind ofly not increase any equipment producing on the Equipment Foundations of 500 type oligomeric isomaltoses, and the zymin consumption is few, utilizes the processing method of cheap distiller's yeast production high purity (900 type) oligomeric isomaltose.
For achieving the above object, the technical scheme taked of the present invention is:
A kind of processing method of utilizing distiller's yeast production high purity oligomeric isomaltose is characterized in that this method may further comprise the steps:
A, size mixing: W-Gum is added deionized water furnishing concentration 17Be '～21Be ', add CaCL again
2Make Ca
2+Concentration at 50～80ppm, use the Na of mass percent concentration 20%～30% then
2CO
3Solution is regulated between pH value to 5.8～6.2;
B, liquefaction: in mixing up the emulsion of slurry, calculate the high temperature resistant α-Dian Fenmei 0.3～0.5kg/t of adding with dry starch, the steam ejection liquefaction temperature is 104～108 ℃ for the first time, injector admission pressure 0.3～0.4MPa, injector feed pressure 0.35～0.55MPa, pressure-bearing pressure tank≤0.1MPa, insulation liquefaction 50～80 minutes; The steam ejection liquefaction temperature is 130～140 ℃ for the second time, and high temperature is kept pressure tank 0.20～0.25MPa, pressure-bearing 3～6 minutes, flash cooling, liquefaction terminal point liquefier iodine examination reddish-brown, liquefaction DE value 15%～18%;
C, saccharification: liquefier is cooled to 54～58 ℃ by plate-type heat exchanger, enter saccharifying tank and regulate pH value to 5.4～5.6, calculate adding fungal enzyme 0.2～0.5kg/t with dry starch, stir saccharification after 15～30 minutes, started in per 1.8～2.2 hours and stir once and detected temperatures and pH value, it is 52%～58% that saccharification time 16～20h surveys saccharification DE value, promptly stops saccharification, be warmed up to 80～82 ℃, be incubated 15～25 minutes enzymes that go out;
D, change glycosides: liquid glucose temperature regulation to 55～60 ℃, pH value that saccharification rest goes out behind the enzyme are adjusted to 5.4～5.6, calculate adding glucose transglucosidase 0.5～1.0kg/t with dry starch and change branch, change glycosides time 24～36h, terminal point DE value is 45%～50%, the mass percent concentration of isomaltose+panose+Isomaltotriose 〉=30%;
E, distiller's yeast hydrolysis purify: earlier distiller's yeast is 30%～50% in the DE value, concentration adjusts to 6%～15%, carry out 28～32 minutes domestication in 1: 20 ratio under 38～42 ℃ of syrup conditions of temperature, through the distiller's yeast of too high sugar domestication by mass percentage concentration 0.5%～1.0% scope add in the liquid glucose after changeing glycosides, 38～42 ℃ of temperature, hydrolysis purification 36～48h, final DE value is 34～38%, the mass percent concentration of isomaltose+panose+Isomaltotriose 〉=48% is warmed up to 68～72 ℃;
F, filtration, decolouring: liquid glucose is in pH value 4.6～5.0,70～75 ℃ of filtrations that remove the gred earlier of temperature; During decolouring, once decolouring adds the dried activated carbon of 2～3kg/t, and secondary decolourization adds the dried activated carbon of 4～5kg/t;
G, refining: utilize ion exchange principle that its salt is removed from handing over;
H, concentrated: concentrate with the vacuum drop film evaporator, vapor pressure is controlled at 0.3～0.4MPa relatively, and mass percent concentration is controlled at 75%～76%, promptly makes high purity (900 type) oligomeric isomaltose slurry.
In the above-mentioned processing method, step h concentrates with the vacuum drop film evaporator, mass percent concentration is controlled at 42%～46%, spray-dried again, vapor pressure is controlled at 0.7～0.9MPa, the clarifixator pressure-controlling is at 6～14MPa, and moisture content of finished products is controlled at 4.0%～4.5%, promptly makes high purity (900 type) oligomeric isomaltose powder.
The processing method of production high purity oligomeric isomaltose provided by the invention, use distiller's yeast and contained the hydrolytic action of plurality of enzymes preparation and its fermentative action, utilize its hydrolytic action can be with the further hydrolysis of the macromole in low-purity oligomeric isomaltose sugar, make its saccharification, transglucosidase preparation consumption reduces relatively; Utilize its fermentative action with fermentations such as its glucose, maltose simultaneously, and then the content that improves branched sugars such as isomaltose, panose, Isomaltotriose is produced highly purified oligomeric isomaltose.This processing method does not need newly-increased any equipment on the basis that produces 500 type oligomeric isomaltoses, utilize inexpensive distiller's yeast, saccharification, transglucosidase preparation have been saved, the oligomeric isomaltose of having purified that ferments simultaneously, low production cost, production method is simple, is fit to suitability for industrialized production.
Description of drawings is the process flow sheet of the embodiment of the invention.
Embodiment as shown in the figure, the implementation step of the processing method of distiller's yeast production high purity oligomeric isomaltose of utilizing provided by the invention is:
A, size mixing: W-Gum is added deionized water furnishing concentration 19Be ', add CaCL again
2Make Ca
2+Concentration at 65ppm, be 25% Na then with mass percent concentration
2CO
3Solution is regulated pH value to 6;
B, liquefaction: in mixing up the emulsion of slurry, add high temperature resistant α-Dian Fenmei 0.4kg/t (calculating with dry starch), the steam ejection liquefaction temperature is 106 ℃ for the first time, injector admission pressure 0.35MPa, injector feed pressure 0.45MPa, pressure-bearing pressure tank≤0.1MPa, insulation liquefaction 65 minutes; The steam ejection liquefaction temperature is 135 ℃ for the second time, and high temperature is kept pressure tank 0.25MPa, pressure-bearing 5 minutes, flash cooling, liquefaction terminal point liquefier iodine examination reddish-brown, liquefaction DE value 17%;
C, saccharification: liquefier is cooled to 56 ℃ by plate-type heat exchanger, enter saccharifying tank and regulate pH value to 5.5, add fungal enzyme 0.35kg/t (calculating) with dry starch, stir saccharification after 20 minutes, opened stirring in per 2 hours once and detected temperatures and pH value, saccharification time 18h, surveying saccharification DE value is 56%, promptly stop saccharification, be warmed up to 81 ℃, be incubated 20 minutes enzymes that go out;
D, change glycosides: the saccharification rest liquid glucose temperature regulation to 58 ℃ behind the enzyme of going out, pH value is adjusted to 5.5, add glucose transglucosidase 0.75kg/t (calculating) and change branch with dry starch, change glycosides time 30h, terminal point DE value is 47%, the mass percent concentration of isomaltose+panose+Isomaltotriose 〉=30%;
E, distiller's yeast hydrolysis purify: earlier distiller's yeast is 50% in the DE value, carries out 30 minutes domestication in 1: 20 ratio under the concentration 10%, 40 ℃ of syrup conditions of temperature, through the distiller's yeast of too high sugar domestication by mass percentage concentration 0.75% scope add in the liquid glucose after changeing glycosides, 40 ℃ of temperature, hydrolysis purification 42h, final DE value is 36%, the mass percent concentration of isomaltose+panose+Isomaltotriose 〉=48% is warmed up to 70 ℃;
F, filtration, decolouring: liquid glucose is in pH value 4.8,72 ℃ of filtrations that remove the gred earlier of temperature; During decolouring, once decolouring adds the dried activated carbon of 2.5kg/t, and secondary decolourization adds the dried activated carbon of 4.5kg/t;
G, refining: utilize ion exchange principle that its salt is removed from handing over;
H, concentrated: concentrate with the vacuum drop film evaporator, vapor pressure is controlled at 0.35MPa relatively, and mass percent concentration is controlled at 75%, and packing promptly makes high purity 900 type oligomeric isomaltoses slurry.
Produce the oligomeric isomaltose powder according to above-mentioned steps, after concentrating with the vacuum drop film evaporator, mass percent concentration is controlled at 44%, spray-dried again, vapor pressure is controlled at 0.8MPa, the clarifixator pressure-controlling is at 10MPa, and moisture content of finished products is controlled at below 4.5%, promptly makes high purity 900 type oligomeric isomaltose powder.
The above only is one embodiment of the present of invention.Be noted that the present invention is not limited to above-mentioned processing parameter; for the person of ordinary skill of the art; under the enlightenment that does not break away from technical solution of the present invention, on its processing step, can also make other and be equal to replacement and improvement, all should think and drop within protection scope of the present invention.
Claims (2)
1, a kind of processing method of utilizing distiller's yeast production high purity oligomeric isomaltose is characterized in that this method may further comprise the steps:
A, size mixing: W-Gum is added deionized water furnishing concentration 17Be '～21Be ', add CaCL again
2Make Ca
2+Concentration at 50～80ppm, use the Na of mass percent concentration 20%～30% then
2CO
3Solution is regulated between pH value to 5.8～6.2;
B, liquefaction: in mixing up the emulsion of slurry, calculate the high temperature resistant α-Dian Fenmei 0.3～0.5kg/t of adding with dry starch, the steam ejection liquefaction temperature is 104～108 ℃ for the first time, injector admission pressure 0.3～0.4MPa, injector feed pressure 0.35～0.55MPa, pressure-bearing pressure tank≤0.1MPa, insulation liquefaction 50～80 minutes; The steam ejection liquefaction temperature is 130～140 ℃ for the second time, and high temperature is kept pressure tank 0.20～0.25MPa, pressure-bearing 3～6 minutes, flash cooling, liquefaction terminal point liquefier iodine examination reddish-brown, liquefaction DE value 15%～18%;
C, saccharification: liquefier is cooled to 54～58 ℃ by plate-type heat exchanger, enter saccharifying tank and regulate pH value to 5.4～5.6, calculate adding fungal enzyme 0.2～0.5kg/t with dry starch, stir saccharification after 15～30 minutes, started in per 1.8～2.2 hours and stir once and detected temperatures and pH value, it is 52%～58% that saccharification time 16～20h surveys saccharification DE value, promptly stops saccharification, be warmed up to 80～82 ℃, be incubated 15～25 minutes enzymes that go out;
D, change glycosides: liquid glucose temperature regulation to 55～60 ℃, pH value that saccharification rest goes out behind the enzyme are adjusted to 5.4～5.6, calculate adding glucose transglucosidase 0.5～1.0kg/t with dry starch and change branch, change glycosides time 24～36h, terminal point DE value is 45%～50%, the mass percent concentration of isomaltose+panose+Isomaltotriose 〉=30%;
E, distiller's yeast hydrolysis purify: earlier distiller's yeast is 30%～50% in the DE value, concentration adjusts to 6%～15%, carry out 28～32 minutes domestication in 1: 20 ratio under 38～42 ℃ of syrup conditions of temperature, through the distiller's yeast of too high sugar domestication by mass percentage concentration 0.5%～1.0% scope add in the liquid glucose after changeing glycosides, 38～42 ℃ of temperature, hydrolysis purification 36～48h, final DE value is 34～38%, the mass percent concentration of isomaltose+panose+Isomaltotriose 〉=48% is warmed up to 68～72 ℃;
F, filtration, decolouring: liquid glucose is in pH value 4.6～5.0,70～75 ℃ of filtrations that remove the gred earlier of temperature; During decolouring, once decolouring adds the dried activated carbon of 2～3kg/t, and secondary decolourization adds the dried activated carbon of 4～5kg/t;
G, refining: utilize ion exchange principle that its salt is removed from handing over;
H, concentrated: concentrate with the vacuum drop film evaporator, vapor pressure is controlled at 0.3～0.4MPa relatively, and mass percent concentration is controlled at 75%～76%, promptly makes high purity oligomeric isomaltose slurry.
2, the processing method of utilizing distiller's yeast production high purity oligomeric isomaltose according to claim 1, it is characterized in that in the step h of this processing method, concentrate with the vacuum drop film evaporator, mass percent concentration is controlled at 42%～46%, spray-dried again, vapor pressure is controlled at 0.7～0.9MPa, and the clarifixator pressure-controlling is at 6～14MPa, moisture content of finished products is controlled at 4.0%～4.5%, promptly makes high purity oligomeric isomaltose powder.
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| CN101126109B (en) * | 2007-09-25 | 2010-11-10 | 中粮融氏生物科技有限公司 | Production technique for corn starch sugar |
| CN102061343A (en) * | 2010-11-14 | 2011-05-18 | 河南永昌飞天淀粉糖有限公司 | Novel process for purifying and recovering crystalline sugar mother liquor |
| CN101117647B (en) * | 2007-09-25 | 2011-05-25 | 中粮融氏生物科技有限公司 | Process for corn starch sugar by enzyme method |
| CN101429528B (en) * | 2007-11-07 | 2011-12-07 | 安庆堂 | Method for direct production of gluconic acid sodium salt with dry method |
| CN102321686A (en) * | 2011-09-14 | 2012-01-18 | 中粮生物化学（安徽）股份有限公司 | Processing method and application of starch raw material |
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| CN102690851A (en) * | 2011-03-23 | 2012-09-26 | 山东百龙创园生物科技有限公司 | Method for producing oligoisomaltose by maltose |
| CN102757990A (en) * | 2012-06-30 | 2012-10-31 | 保龄宝生物股份有限公司 | Preparation method of high-purity isomaltose hypgather |
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| CN103911409A (en) * | 2014-04-09 | 2014-07-09 | 孝感市惠隆油脂有限公司 | Preparation method of malt syrup |
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| CN101117647B (en) * | 2007-09-25 | 2011-05-25 | 中粮融氏生物科技有限公司 | Process for corn starch sugar by enzyme method |
| CN101126109B (en) * | 2007-09-25 | 2010-11-10 | 中粮融氏生物科技有限公司 | Production technique for corn starch sugar |
| CN101429528B (en) * | 2007-11-07 | 2011-12-07 | 安庆堂 | Method for direct production of gluconic acid sodium salt with dry method |
| CN102061343A (en) * | 2010-11-14 | 2011-05-18 | 河南永昌飞天淀粉糖有限公司 | Novel process for purifying and recovering crystalline sugar mother liquor |
| CN102690851A (en) * | 2011-03-23 | 2012-09-26 | 山东百龙创园生物科技有限公司 | Method for producing oligoisomaltose by maltose |
| CN102352396B (en) * | 2011-09-01 | 2013-11-13 | 河南飞天农业开发股份有限公司 | Method of producing functional starch sugar through wheat starch |
| CN102352396A (en) * | 2011-09-01 | 2012-02-15 | 河南永昌飞天淀粉糖有限公司 | Method of producing functional starch sugar through wheat starch |
| CN102321686B (en) * | 2011-09-14 | 2015-01-07 | 中粮生物化学（安徽）股份有限公司 | Processing method and application of starch raw material |
| CN102321686A (en) * | 2011-09-14 | 2012-01-18 | 中粮生物化学（安徽）股份有限公司 | Processing method and application of starch raw material |
| CN102757990A (en) * | 2012-06-30 | 2012-10-31 | 保龄宝生物股份有限公司 | Preparation method of high-purity isomaltose hypgather |
| CN102965284A (en) * | 2012-11-27 | 2013-03-13 | 中粮生化能源（公主岭）有限公司 | Dual-purpose preparation process and equipment for maltodextrin and oligomerization isomaltose |
| CN102965284B (en) * | 2012-11-27 | 2014-09-03 | 中粮生化能源（公主岭）有限公司 | Dual-purpose preparation process and equipment for maltodextrin and oligomerization isomaltose |
| CN103039777A (en) * | 2012-12-21 | 2013-04-17 | 保龄宝生物股份有限公司 | Preparation method of syrup special for moon cake |
| CN103039777B (en) * | 2012-12-21 | 2014-01-15 | 保龄宝生物股份有限公司 | Preparation method of syrup special for moon cake |
| CN103911409A (en) * | 2014-04-09 | 2014-07-09 | 孝感市惠隆油脂有限公司 | Preparation method of malt syrup |
| CN104171800A (en) * | 2014-08-08 | 2014-12-03 | 山东百龙创园生物科技有限公司 | Isomaltooligosacharide-fructooligosaccharide composite particle and preparation method thereof |
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| CN105294783B (en) * | 2015-09-21 | 2018-10-12 | 山东百龙创园生物科技股份有限公司 | A kind of method of polymerization production oligoisomaltose |
| CN108265093A (en) * | 2017-12-27 | 2018-07-10 | 河南飞天农业开发股份有限公司 | A kind of syrup dedicated production technology of toffee |
| CN107937456A (en) * | 2017-12-29 | 2018-04-20 | 广州华汇生物实业有限公司 | A kind of preparation method of slow digestible saccharide |
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