CN104137949B - The application process of macleaya cordata plant in feed - Google Patents
The application process of macleaya cordata plant in feed Download PDFInfo
- Publication number
- CN104137949B CN104137949B CN201310303875.4A CN201310303875A CN104137949B CN 104137949 B CN104137949 B CN 104137949B CN 201310303875 A CN201310303875 A CN 201310303875A CN 104137949 B CN104137949 B CN 104137949B
- Authority
- CN
- China
- Prior art keywords
- macleaya cordata
- feed
- total amount
- plant
- content meter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Fodder In General (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides the application of macleaya cordata plant in feed, the broiler diets of described macleaya cordata plant in feed, with sanguinarine content meter, total amount is no more than 9.8ppm; With Chelerythrine content meter, total amount must not more than 5.0ppm; With Biflorine content meter, total amount must not more than 7.5ppm; In former allocryptopine, total amount must not more than 6.8ppm.One aspect of the present invention can reduce manpower and materials in Macleaya cordata alkaloids leaching process and environmental pollution, on the other hand, can ensure again the optimal dose of Macleaya cordata alkaloids in feed, plays class antibiotic and somatotrophic effectiveness.The recent tendency that the present invention meets development in science and technology, economy and environment protects demand, to the antibiotic abuse of control, reduces feed cost, ensures that the safety of meat product and minimizing environmental pollution have actual and great meaning.
Description
Technical field
The present invention relates to feed additive field, be specifically related to macleaya cordata plant (containing the seed) application in feed.
Background technology
In current aquaculture, antibiotic a large amount of use, though improve culture benefit, the drug resistance that result also in germ strengthens, medicament residue exceeds standard and the problems such as environmental pollution, and cultivation dealer and feed manufacturer are gradually then demand replaces antibiotic product and raw material.Macleaya cordata is one of Chinese herbal medicine of Chinese tradition, and its extract has been widely used in replacement antibiotic at home and abroad, promotes animal health and growth.
Macleaya cordata system Papaveraceae Macleaya plant, is distributed widely in China East China, south China and southwest.Isoquinoline alkaloid is rich in the plant of macleaya cordata and seed, as sanguinarine, Chelerythrine, opium alkali, cryptocavine, coptisine etc., these alkaloids have stronger inhibitory action to gram-positive bacteria, Gram-negative bacteria and Leptospira, also show very strong killing action to parasite.The extraction process of Macleaya cordata alkaloids and using method have been tending towards ripe, and it is widely used in Biological Pesticide, parasite insecticide, wound antiphlogistic, toothpaste additive, cancer therapy drug etc.
In animal productiong, the use research of macleaya cordata extracts also has report, and the veterinary drug that Macleaya cordata alkaloids is made has been applied to the treatment various diseases such as piglet yellow-white dysentery, enteritis and parasite.And macleaya cordata extracts makes an addition in feed, the intestinal environment of animal can be improved on the one hand, suppress the propagation of harmful bacteria; On the other hand, the amino acid that can watch for animals in enteron aisle, reduces amino acid whose degraded in alimentary canal, improves protein and amino acid whose utilization rate, also participate in the hormone control of body, increases the accumulation of body protein; Contain the lubricant component up to 35.4% simultaneously in macleaya cordata seed, contribute to the palatability improving feed, improve feed intake.Therefore, the macleaya cordata extracts be applied in feed has replacement antibiotic, promotes feed intake and growth, reduces the effect of Animal diseases.
Macleaya cordata plant (containing seed) possesses abundant raw material, technique simplifies the advantage with low cost, its seed is extracted the grease that obtains by existing practitioner and alkaloid is used in feed, but the process of extraction seed oil is more complicated, needs certain equipment, wastes time and energy.And if the macleaya cordata plant (containing seed) without chemical extraction is directly applied in feed, again due to the difference of the factors such as the place of production, season and weather, additive effect is unstable, is difficult to be formed clear and definite and effective application method.
Summary of the invention
The object of the invention is for the problems referred to above, develop and a kind ofly apply the application process of macleaya cordata in feed that be simple, effect stability.
For achieving the above object, technical scheme provided by the invention is, the application of macleaya cordata plant in feed, the broiler diets of described macleaya cordata plant in feed, and with sanguinarine content meter, total amount is no more than 9.8ppm; With Chelerythrine content meter, total amount is no more than 5.0ppm; With Biflorine content meter, total amount is no more than 7.5ppm; In former allocryptopine, total amount is no more than 6.8ppm.
Preferably, the broiler diets of described macleaya cordata plant in feed, with sanguinarine content meter, total amount is 0.25 ~ 5.0ppm; With Chelerythrine content meter, total amount is 0.02 ~ 1.5ppm; With Biflorine content meter, total amount is 0.08 ~ 3.0ppm; In former allocryptopine, total amount is 0.06 ~ 2.0ppm.
The broiler diets of described macleaya cordata plant in feed, with sanguinarine content meter, total amount is 0.4 ~ 2.5ppm; With Chelerythrine content meter, total amount is 0.03 ~ 1.0ppm; With Biflorine content meter, total amount is 0.1 ~ 1.5ppm; In former allocryptopine, total amount is 0.08 ~ 1.0ppm.
Preferably, described macleaya cordata plant comprises its seed.
Preferably, described macleaya cordata plant, after super-dry, pulverizing, detects its alkaloidal content, then according to the addition of its alkaloidal concentration determination macleaya cordata plant in feed.Wherein, described macleaya cordata plant comprises seed.
All contain the alkaloid (as shown in table 1) of higher concentration in the whole strain of macleaya cordata, be enough to directly use as feed addictive, the alkaloid wherein in seed is the highest, even can reach 5%, is secondly root, leaf and bar.
The content of main alkaloid in table 1 macleaya cordata plant (containing seed)
| Sanguinarine, % | Chelerythrine, % | Biflorine, % | Former allocryptopine, % | |
| Seed | 0.4~2.1 | 0.43~0.7 | 0.5~1.37 | 0.3~0.69 |
| Root | 0.28~0.71 | 0.17~0.65 | 0.4~1.19 | 0.36~0.97 |
| Leaf | 0.3~1.52 | 0.1~0.48 | 0.5~1.47 | 0.4~1.51 |
| Bar | 0.20~0.82 | 0.17~1.10 | 0.25~0.79 | 0.17~0.80 |
The present invention is based on ripe Macleaya cordata alkaloids to extract and detection technique, using method as the macleaya cordata plant (containing seed) of feed addictive is proposed: before Chinese herbal medicine macleaya cordata plant (containing seed) makes an addition to feed, first detect accurately, then foundation wherein one or several effective alkaloidal content, determine macleaya cordata plant (containing the seed) additive capacity in feed, with substitute antibiotics and promotion growth of animal.
Technical scheme of the present invention, mainly contains the advantage of following several aspect: 1, can be used in feed alkaloid by accurate quantification, the invalid interpolation avoiding low dosage to cause, and the excessive toxic action caused; 2, the addition of macleaya cordata plant (containing seed) additive in feed is little; 3, reduce antibiotic interpolation in feed, ensure that animal health grows fast, improve the security of feed and livestock products simultaneously; 4, macleaya cordata is without chemical reagent leaching process, avoids the energy waste in extraction and process, environmental pollution, and the toxicity that the excessive use of extract causes, and reduces feed cost simultaneously, improves cultivation income.
The present invention is intended to use macleaya cordata plant (containing seed) to reduce antibiotic use in pig industry, the manpower and materials in Macleaya cordata alkaloids leaching process and environmental pollution can be reduced on the one hand, on the other hand, the optimal dose of Macleaya cordata alkaloids in feed can be ensured again, play class antibiotic and somatotrophic effectiveness, effect stability is guaranteed.The recent tendency that the present invention meets development in science and technology, economy and environment protects demand, to the antibiotic abuse of control, reduces feed cost, ensures that the safety of meat product and minimizing environmental pollution have actual and great meaning.
Detailed description of the invention
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read content of the present invention, these equivalent form of values fall within the application's limited range equally.
Embodiment 1, macleaya cordata plant (containing seed) alkaloid composition and content analysis
The leaf bar of the macleaya cordata plant that 10 ~ November plucks and inferior seed, drying pulverizes the powder and homogeneous mixture thereof that obtain, its crude fat content is detected by National Standard Method, after alkaloid then adopts acidic ethanol method to extract, sanguinarine and chelerythrine alkali content is detected with high performance liquid chromatograph, all contain the alkaloid of higher concentration in the leaf bar, seed and composition thereof of result display macleaya cordata plant, and be also rich in a large amount of greases in seed, concrete data are as shown in table 2.
The content of crude fat and sanguinarine in table 2 macleaya cordata leaf bar and seed
| Macleaya cordata leaf bar | Macleaya cordata seed powder | Macleaya cordata leaf bar seed | |
| Crude fat, % | 9.37 | 29.32 | 21.71 |
| Sanguinarine, % | 1.99 | 0.27 | 0.99 |
| Chelerythrine, % | 0.13 | 0.1 | 0.06 |
Embodiment 2,
Preferred macleaya cordata plant (containing seed), drying is pulverized, the macleaya cordata seed leaf bar mixture made, after extracting by acidic ethanol method, detect that its sanguinarine content is 1.2% with high performance liquid chromatography, be as the criterion with sanguinarine concentration 0.4 ~ 2.5ppm final in feed, macleaya cordata seed leaf bar additive is made an addition to pig starter feed with the ratio of 50ppm, mix step by step, piglet of feeding, observe feeding effect.
This test chooses that 42 ± 2 ages in days, body weight are close, body condition healthy Du ╳ Chang ╳ Dasanyuan hybridization child care piglet 115, be divided into three groups at random, often organize 3 repetitions (hurdle), eachly repeat about 13, control group 1: basal diet of feeding (control group), control group 2: basal diet+90ppm import macleaya cordata of feeding extracts preparation, test group: basal diet+50ppm macleaya cordata plant (containing seed) of feeding, feeding and management flow process routinely, feed 21 days, every day observed and recorded pig mental status and to search for food situation, weigh in the morning of on-test and end on an empty stomach.The weightening finish of test gained, situation of searching for food and health status are as shown in table 3.
Table 3 macleaya cordata plant (containing seed) is on piglet growth and healthy impact
| Control group 1 | Control group 2 | Test group | |
| 42 age in days head numbers | 37 | 39 | 39 |
| 63 age in days head numbers | 36 | 35 | 36 |
| 42 age in days counterpoises, kg | 12.91±0.64 | 13.05±0.27 | 12.93±0.55 |
| 63 age in days counterpoises, kg | 19.62±0.65 | 20.55±0.19 | 20.77±0.61 |
| Average daily gain, g | 318.57±2.08 | 336.64±6.02 | 376.57±58.09 |
| Average daily ingestion amount, g | 607.15±17.49 | 607.76±26.01 | 608.28±18.72 |
| Feedstuff-meat ratio | 1.91±0.07 | 1.80±0.07 | 1.61±0.13 |
| Diarrhea rate, % | 4.83±1.69 | 4.37±1.90 | 2.37±0.26 |
Experimental group number is according to the show: add 90ppm macleaya cordata in daily ration and extract the control group 2 of preparation and add the test group of 50ppm macleaya cordata plant (containing seed), daily gain, the daily ingestion amount of piglet are all improved, and feedstuff-meat ratio and diarrhea rate all obviously reduce.Illustrate that macleaya cordata plant (containing seed) additive has good growth promotion, improves efficiency of feed utilization and anti-CaM antibody, its growth promotion and only dysentery effect are better than the effect extracting preparation.Wherein, test group and control group 2 are more or less the same in average daily ingestion amount, but in average daily gain, test group adds 18.2% than control group 1, add about 11.8% than control group 2, in feedstuff-meat ratio, test group reduces 0.3 than control group 1, about decrease 15.7%, about decrease 10.5% than control group 2.Diarrhea rate, test group is than control group 1 and control group 2 reduce 50.9% and 45.8% respectively.
Embodiment 3,
Preferred macleaya cordata plant (containing seed), drying is pulverized, the macleaya cordata seed leaf bar mixture made, after extracting by acidic ethanol method, detect the content of Chelerythrine is 0.07% with high performance liquid chromatography, the chelerythrine alkali concn that macleaya cordata extracts preparation is 0.09%, in Chelerythrine ultimate density 0.03 ~ 1.0ppm in feed, macleaya cordata seed leaf bar additive is extracted preparation with the ratio of 50ppm, macleaya cordata and makes an addition to pig starter feed with the ratio of 90ppm, mix step by step, to feed piglet, observe feeding effect.
This test chooses that 42 ± 2 ages in days, body weight are close, body condition healthy Du ╳ Chang ╳ Dasanyuan hybridization child care piglet 90, be divided into three groups at random, often organize 3 repetitions (hurdle), each repetition 10, control group 1: basal diet of feeding (control group), control group 2: basal diet+90ppm import macleaya cordata of feeding extracts preparation, test group: basal diet+50ppm macleaya cordata plant (containing seed) of feeding, feeding and management flow process routinely, feed 21 days, every day observed and recorded pig mental status and to search for food situation, weigh in the morning of on-test and end on an empty stomach.The weightening finish of test gained, situation of searching for food and health status are as shown in table 4.
Table 4 macleaya cordata plant (containing seed) is to the growth-promoting effect of piglet
| Control group 1 | Control group 2 | Test group | |
| 42 age in days head numbers | 30 | 30 | 30 |
| 63 age in days head numbers | 29 | 30 | 30 |
| 42 age in days counterpoises, kg | 13.52±0.34 | 13.23±0.37 | 12.54±0.55 |
| 63 age in days counterpoises, kg | 20.25±0.55 | 20.71±0.29 | 20.78±0.56 |
| Average daily gain, g | 318.76±2.98 | 334.41±7.12 | 377.95±6.19 |
| Average daily ingestion amount, g | 609.21±17.49 | 518.79±8.01 | 608.31±18.72 |
| Feedstuff-meat ratio | 1.91±0.08 | 1.74±0.05 | 1.61±0.06 |
| Diarrhea rate, % | 4.91±1.49 | 4.17±1.79 | 2.40±0.26 |
Experimental group number is according to the show: add the daily gain that 90ppm macleaya cordata extracts the control group 2 of preparation and the test group piglet of 50ppm macleaya cordata plant (containing seed) and be all improved, feedstuff-meat ratio and diarrhea rate have the trend of reduction, and the growth achievement of test group piglet is far away higher than control group 2, illustrate that macleaya cordata extracts and plant (containing seed) additive have and promote piglet growth, raising efficiency of feed utilization and anti-diarrhea effect, and the effect of macleaya cordata plant (containing seed) additive is better than extracting preparation effect.Wherein, although the average initial weight of test group and control group 2 piglet is lower than control group 1, but body weight exceeds 0.46kg and 0.53kg than control group respectively during off-test, although the average daily ingestion amount of test group piglet is in a slight decrease, but daily gain improves 18.57% than control group 1, improve 13.02% than control group 2, feedstuff-meat ratio reduces by 0.3 and 0.13 than control group 1 and control group 2 respectively.Relatively showing of embodiment, the feed adding macleaya cordata plant (containing seed) has a clear superiority in than the control group not adding macleaya cordata in average daily gain, feedstuff-meat ratio, diarrhea rate, promotes the quick growth of cultivated animals, improves efficiency of feed utilization and have positive using value in economizing on resources in breeding production.The effect that the effect of adding macleaya cordata plant (containing seed) extracts preparation with interpolation macleaya cordata is consistent; even extraction preparation will be exceeded in a lot; and consumption is less; processing is simplified and few chemicals and pollution thereof in process; therefore more save economically; more favourable in environmental protection, the toxicity brought because of excessive use can also be avoided.
The application of macleaya cordata plant in feed, the broiler diets of described macleaya cordata plant in feed, with sanguinarine content meter, total amount is no more than 9.8ppm; With Chelerythrine content meter, total amount must not more than 5.0ppm; With Biflorine content meter, total amount must not more than 7.5ppm; In former allocryptopine, total amount must not more than 6.8ppm.
One aspect of the present invention can reduce manpower and materials in Macleaya cordata alkaloids leaching process and environmental pollution, on the other hand, can ensure again the optimal dose of Macleaya cordata alkaloids in feed, and play class antibiotic and somatotrophic effectiveness, effect stability is guaranteed.The recent tendency that the present invention meets development in science and technology, economy and environment protects demand, to the antibiotic abuse of control, reduces feed cost, ensures that the safety of meat product and minimizing environmental pollution have actual and great meaning.
Be more than the description to the embodiment of the present invention, by the above-mentioned explanation to the disclosed embodiments, professional and technical personnel in the field realized or uses the present invention.To be apparent for those skilled in the art to the multiple amendment of these embodiments, General Principle as defined herein can without departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention can not be restricted to these embodiments shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (5)
1. the application of macleaya cordata plant in feed, is characterized in that, the broiler diets of described macleaya cordata plant in feed, and with sanguinarine content meter, total amount is no more than 9.8ppm; With Chelerythrine content meter, total amount is no more than 5.0ppm; With Biflorine content meter, total amount is no more than 7.5ppm; In former allocryptopine, total amount is no more than 6.8ppm.
2. the application of macleaya cordata plant in feed as claimed in claim 1, it is characterized in that, the broiler diets of described macleaya cordata plant in feed, with sanguinarine content meter, total amount is 0.25 ~ 5.0ppm; With Chelerythrine content meter, total amount is 0.02 ~ 1.5ppm; With Biflorine content meter, total amount is 0.08 ~ 3.0ppm; In former allocryptopine, total amount is 0.06 ~ 2.0ppm.
3. the application of macleaya cordata plant in feed as claimed in claim 1, it is characterized in that, the broiler diets of described macleaya cordata plant in feed, with sanguinarine content meter, total amount is 0.4 ~ 2.5ppm; With Chelerythrine content meter, total amount is 0.03 ~ 1.0ppm; With Biflorine content meter, total amount is 0.1 ~ 1.5ppm; In former allocryptopine, total amount is 0.08 ~ 1.0ppm.
4. the application of macleaya cordata plant in feed as described in as arbitrary in claim 1 ~ 3, it is characterized in that, described macleaya cordata plant comprises its seed.
5. the application of macleaya cordata plant in feed as described in as arbitrary in claim 1 ~ 3, it is characterized in that, described macleaya cordata plant, after super-dry, pulverizing, detects its alkaloidal content, then according to the addition of its alkaloidal concentration determination macleaya cordata plant in feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310303875.4A CN104137949B (en) | 2013-07-19 | 2013-07-19 | The application process of macleaya cordata plant in feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310303875.4A CN104137949B (en) | 2013-07-19 | 2013-07-19 | The application process of macleaya cordata plant in feed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104137949A CN104137949A (en) | 2014-11-12 |
| CN104137949B true CN104137949B (en) | 2016-03-23 |
Family
ID=51847432
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310303875.4A Active CN104137949B (en) | 2013-07-19 | 2013-07-19 | The application process of macleaya cordata plant in feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104137949B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105192406A (en) * | 2015-10-15 | 2015-12-30 | 无锡市南阳湖水产科技有限公司 | Breeding feed for catfish aquatic products |
| CN113318150A (en) * | 2021-04-20 | 2021-08-31 | 铜仁职业技术学院 | Application of macleaya cordata extract in preparation of oral liquid for promoting animal growth |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530475A (en) * | 2009-04-17 | 2009-09-16 | 湖南美可达生物资源有限公司 | Application of pink plumepoppy herb extract in veterinary drugs of economic animals |
-
2013
- 2013-07-19 CN CN201310303875.4A patent/CN104137949B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530475A (en) * | 2009-04-17 | 2009-09-16 | 湖南美可达生物资源有限公司 | Application of pink plumepoppy herb extract in veterinary drugs of economic animals |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104137949A (en) | 2014-11-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101843298B (en) | Plant composite extract for feed additive, and preparation method and application thereof | |
| CN103385367B (en) | Piglet anti-diarrhea composite Chinese medicinal herb feed additive | |
| CN105010884A (en) | Environment-friendly pig feed efficient in growth promotion and preparation method thereof | |
| CN102132780B (en) | Feed additive for chickens | |
| CN105746885A (en) | Mixed type feed additive and application thereof | |
| CN102524589B (en) | Weaned pig feed as well as preparation method and application thereof | |
| CN102613436A (en) | Chinese herbal medicine additive for meat duck feed and use method thereof | |
| CN1082337C (en) | Contain the fodder compound and the nourishing healthy agent of triterpenes saponin extract | |
| CN105661076A (en) | Pig breeding feed formulation and breeding method thereof | |
| CN103202409A (en) | Special feed additive for grass carp | |
| CN104137949B (en) | The application process of macleaya cordata plant in feed | |
| CN109007344A (en) | A kind of compound feed additive of anti-cow heat stress and preparation method thereof | |
| Silva et al. | Effects of dietary brown propolis on nutrient intake and digestibility in feedlot lambs | |
| CN102210404B (en) | Feed additive capable of effectively enhancing immunologic function and improving pork quality | |
| EP1317187B1 (en) | Feedstuff or a feedstuff additive used as a performance promoter or as an appetite promoter for domestic animals | |
| Moradzadeh‐Somarin et al. | Valorization of dietary edible mushrooms waste: chemical and physical properties, nutrient digestibility, microbial protein synthesis and nitrogen balance in sheep | |
| CN103892048B (en) | Piglet is calmed the nerves to increase and exempts from growth-promoting feedstuff additive, preparation method and application thereof | |
| KR101427610B1 (en) | Piglet feed additive composition having increasing immunity effect and feed of piglet containing thereof | |
| CN104431374B (en) | A kind of feed addictive for improveing Egg Quality | |
| CN107019099A (en) | A kind of sheep tannin acid type feed formula and its digestion and metabolism assay method | |
| KR101444402B1 (en) | Weaning pig feed additive composition having improving productivity and feed of weaning piglet containing thereof | |
| CN109170344A (en) | A kind of feed and preparation method thereof for improving field and putting chicken disease resistance in a suitable place to breed | |
| CN103202388A (en) | Additive for reducing sheep haemonchus contortus infection and improving feed protein utilization rate and application thereof | |
| CN103404747A (en) | Compound feed containing sunflower disk meal for piglets and preparation method of compound feed | |
| CN103766652A (en) | Natural plant feed additive for improving mutton quality and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent for invention or patent application | ||
| CB02 | Change of applicant information |
Address after: 215437 Jiangsu city of Suzhou province Taicang city Shaxi town Xingang Yuewang Road No. 239 Applicant after: ANYOU BIOTECHNOLOGY GROUP CO., LTD. Address before: 215437 Jiangsu city of Suzhou province Taicang city Shaxi town Xingang Yuewang Road No. 239 Applicant before: Anyou Biological Technology Group Co., Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |