CN107019099A - A kind of sheep tannin acid type feed formula and its digestion and metabolism assay method - Google Patents
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Abstract
The present invention relates to technical field of animal, particularly a kind of sheep tannin acid type feed formula and its digestion and metabolism assay method.Including basal feed and tannic acid, tannic acid is admixed in basal feed, tannic acid addition is 0.1 20gkg‑1, it is more obtained than dispensing that basal feed presses following weight：0.1 0.2 parts of 35 40 parts of corn, 5 10 parts of Soybean Meal, 10 15 parts of alfalfa hay, 25 30 parts of maize straw, 15 20 parts of Barley straw, 0.1 0.2 parts of calcium monohydrogen phosphate, 0.3 0.4 parts of agstone, 0.5 1 parts of salt and premix.Added in the present invention after tannic acid; tannic acid protein complex is formed, in abomasum (pH is 2.5) and small intestine (pH is 8~9), is decomposed by pepsin and trypsase; the small-molecule substance for being easy to absorb is formed, so as to reach the effect of rumen bypass protein protection.Protein content in dairy produce can be improved, thus infers that tannic acid can improve forage protein digesting and assimilating in Contents in Cows.Research finds that tannin acid content is 3.40gkg in diet‑1When it is best to the protecting effect of diet protein.
Description
Technical field
The present invention relates to technical field of animal, particularly a kind of sheep tannin acid type feed formula and its digestion and metabolism
Assay method.
Background technology
Tannic acid (Tannic Acid) is also known as tannic acid, is a kind of polyphenol compound, be present in persimmon, tealeaves, coffee,
In the plant tissues such as pomegranate, sorghum seed, water-soluble tannin is formed when plant is not yet ripe, as the maturation of seed has water-soluble
Property switchs to coagulability.The structure of tannic acid is extremely complex, belongs to Polyhydroxy phenol.Astringent taste in food, feed, generally
It is relevant with tannic acid.Moreover, tannic acid can be combined to form precipitation with protein, alkaloid, pectin etc., therefore recognized always
To be the ANFs in feed.It is appropriate in diet however, due to ruminant special physiological structure and digestive characteristic
Tannic acid is utilized to the protein digestibility of ruminant has certain positive role with environmental protection, is on the one hand to work as rumen ph
For 5~7 when, tannin can with the protein formation compound in plant feed, solubility of the reduction protein in cud and
Surface-active, it is to avoid degraded rapidly by rumen microorganism, increases rumen bypass protein quantity, so as to improve Protein utilization.
But tannin acid content is limited in the plant tissues such as persimmon, tealeaves, coffee, pomegranate, sorghum seed, single pass plant
Tissue intake tannic acid can not realize that the mass of ruminant feeds work, while taking in a large amount of persimmons, tealeaves, coffee, stone
The plant tissues such as pomegranate, sorghum seed easily cause the phase that nutrition is unbalanced, does not in the prior art feed tannic acid mass
Close report.
The content of the invention
The present invention solves one kind can mass production, consumption accurately sheep tannin acid type feed formula and its digestion and metabolism
Assay method.
The technical solution adopted for the present invention to solve the technical problems is as follows：
Tannic acid is admixed in a kind of sheep tannin acid type feed formula, including basal feed and tannic acid, basal feed, it is single
Peaceful acid addition is 0.1-20gkg-1, it is more obtained than dispensing that basal feed presses following weight：35-40 parts of corn, Soybean Meal 5-10
Part, 10-15 parts of alfalfa hay, 25-30 parts of maize straw, 15-20 parts of Barley straw, 0.1-0.2 parts of calcium monohydrogen phosphate, agstone
0.1-0.2 parts of 0.3-0.4 parts, 0.5-1 parts of salt and premix.
The basal feed is made by following weight than dispensing：37.85 parts of corn, 6 parts of Soybean Meal, 12 parts of alfalfa hay,
27 parts of maize straw, 16 parts of Barley straw, 0.17 part of calcium monohydrogen phosphate, 0.33 part of agstone, 0.5 part of salt and premix 0.15
Part.
It is made in the premix by following weight than trace element：60 parts of zinc Zn, 60 parts of iron Fe, Mn40 parts of manganese, copper
12 parts of Cu, 1 part of iodine I, 0.2 part of selenium Se, 0.3 part of cobalt Co.
The tannic acid addition is 3.40gkg-1。
A kind of digestion and metabolism assay method of sheep tannin acid type feed formula, it is characterised in that comprise the following steps：
A, early-stage preparations
Rumen fistula, conventional feed and sheep tannin acid type feed mixing feeding are installed to experiment sheep, and gradually increased continuous
Sheep tannin acid type feed dosage, until complete feeding sheep tannin acid type feed, experiment sheep feeding sheep tannin acid type feed is matched somebody with somebody
Start sampling after Fang Yizhou, day feeding volume is the 3% of body weight, and experiment sheep, which is freely fetched water, drinks；
B, digestion and metabolism sample
Collect full excrement and full urine carries out digest supersession test, the phase of collecting is 5 days, fecaluria discharge rate is collected day by day and feeding is observed
Expect after being mixed, dried and crushed after inventory and surplus doses, full collecting dung, observation dry, organic substance and fiber
The digestion situation of material, and monitoring record calcium Ca, phosphorus P, nitrogen N content；
C, rumen fluid sample
At times collect rumen fluid sample, respectively before food 1h, food after 1h, food after 3h, food after 5h, food after 7h from abdomen capsule
Rumen fluid 50mL is extracted, with pH value is determined, with 4 layers of filtered through gauze, filtrate is collected, filtrate adds saturation HgCl2Solution, in ﹣ 20
DEG C preserve, detection rumen fluid in total nitrogen, ammonia nitrogen NH3- N content；
D, plasma sample
2h after on the feed, gathers sheep jugular vein blood 10mL, adds heparin and shakes up, with 5000r/min centrifugal treating 10min systems
Blood plasma is taken, the urea nitrogen content in 20 DEG C of preservations of ﹣, detection blood plasma.
Full excrement dries the H that mass concentration 10% is added to constant weight at 65 DEG C in the step B after mixing2SO4Solution
Excrement sample is submerged, is refrigerated in the case where 20 DEG C of ﹣, H is added in full urine2SO4Solution, full urine ph values are reduced to less than 3, are refrigerated at 4 DEG C,
Detection records calcium Ca, phosphorus P, nitrogen N content in full excrement and full urine.
Added in the present invention after tannic acid, tannic acid protein complex is formed, in abomasum (pH is 2.5) and small intestine (pH
For 8~9) when, decomposed by pepsin and trypsase, the small-molecule substance for being easy to absorb formed, so as to reach rumen bypass
The effect of protein protection.Protein content in dairy produce can be improved, thus infers that tannic acid can improve forage protein in Contents in Cows
Interior digests and assimilates.Research finds that tannin acid content is 3.40gkg in diet-1When to the protecting effect of diet protein most
It is good.At present, protection of the material rich in tannic acid such as tealeaves, sorghum seed, shrub leaf to ruminant rumen protein is imitated
Really, numerous studies report is had both at home and abroad；And this case is directly using chemical sterling tannic acid (C76H52O64) raised and surveyed
Examination, and then show that addition proportioning carries out mass production.This experiment is using sheep as animal model, it is intended to study addition point in diet
Analyse pure tannic acid (C76H52O64) to the utilization of ruminant animal nutrition substance digestion and the influence of nitrogen metabolism, the pure tannic acid of exploration and analysis
Broiler diets in diet, are sterling tannic acid as ruminant and protect a kind of novel fodder of rumen bypass protein to add
Agent is developed and provides theoretical foundation for production practices.
Embodiment
Tannic acid is admixed in a kind of sheep tannin acid type feed formula, including basal feed and tannic acid, basal feed, it is single
Peaceful acid addition is 0.1-20gkg-1, it is more obtained than dispensing that basal feed presses following weight：35-40 parts of corn, Soybean Meal 5-10
Part, 10-15 parts of alfalfa hay, 25-30 parts of maize straw, 15-20 parts of Barley straw, 0.1-0.2 parts of calcium monohydrogen phosphate, agstone
0.1-0.2 parts of 0.3-0.4 parts, 0.5-1 parts of salt and premix.
The basal feed is made by following weight than dispensing：37.85 parts of corn, 6 parts of Soybean Meal, 12 parts of alfalfa hay,
27 parts of maize straw, 16 parts of Barley straw, 0.17 part of calcium monohydrogen phosphate, 0.33 part of agstone, 0.5 part of salt and premix 0.15
Part.It is made in the premix by following weight than trace element：60 parts of zinc Zn, 60 parts of iron Fe, Mn40 parts of manganese, copper Cu 12
Part, 1 part of iodine I, 0.2 part of selenium Se, 0.3 part of cobalt Co.It is preferred that the tannic acid addition be 3.40gkg-1。
A kind of digestion and metabolism assay method of sheep tannin acid type feed formula, it is characterised in that comprise the following steps：
A, early-stage preparations
Rumen fistula, conventional feed and sheep tannin acid type feed mixing feeding are installed to experiment sheep, and gradually increased continuous
Sheep tannin acid type feed dosage, until complete feeding sheep tannin acid type feed, experiment sheep feeding sheep tannin acid type feed is matched somebody with somebody
Start sampling after Fang Yizhou, day feeding volume is the 3% of body weight, and experiment sheep, which is freely fetched water, drinks；
B, digestion and metabolism sample
Collect full excrement and full urine carries out digest supersession test, the phase of collecting is 5 days, fecaluria discharge rate is collected day by day and feeding is observed
Expect after being mixed, dried and crushed after inventory and surplus doses, full collecting dung, observation dry, organic substance and fiber
The digestion situation of material, and monitoring record calcium Ca, phosphorus P, nitrogen N content；Dried after full excrement is mixed in the step B at 65 DEG C
To constant weight, the H of mass concentration 10% is added2SO4Solution submerges excrement sample, is refrigerated in the case where 20 DEG C of ﹣, and H is added in full urine2SO4Solution,
Full urine ph values are reduced to less than 3, are refrigerated at 4 DEG C, and detection records calcium Ca, phosphorus P, nitrogen N content in full excrement and full urine；
C, rumen fluid sample
At times collect rumen fluid sample, respectively before food 1h, food after 1h, food after 3h, food after 5h, food after 7h from abdomen capsule
Rumen fluid 50mL is extracted, with pH value is determined, with 4 layers of filtered through gauze, filtrate is collected, filtrate adds saturation HgCl2Solution, in ﹣ 20
DEG C preserve, detection rumen fluid in total nitrogen, ammonia nitrogen NH3- N content；
D, plasma sample
2h after on the feed, gathers sheep jugular vein blood 10mL, adds heparin and shakes up, with 5000r/min centrifugal treating 10min systems
Blood plasma is taken, the urea nitrogen content in 20 DEG C of preservations of ﹣, detection blood plasma.
Above-mentioned detection data carry out Latin square variance analysis using the softwares of SPSS 20.0, and Turkey is used during significant difference
Method does Multiple range test, and result of the test is expressed as Mean ± SD.
Contrast test
1.1 experimental design
This experiment uses 4 × 4 Latin―Square designs, from the castrated ram sheep of 4 installation permanence lymphoma stomach fistulization pipes, 2 years old, average body
Weight is 35 ± 2.15kg, and diet cycle period is 16d, preliminary trial period 10d, positive examination phase 6d, the common 64d of full period.According to early stage trial test
As a result, tannic acid maximum additive capacity≤20gkg of sheep feed intake is influenceed-1, this experiment is divided into A, B, C and D group, exists successively
Sterling tannic acid 0,10,15,20gkg are added in Basic drawing-1DM。
1.2 experiment diets and the care of animal
Diet reference Chinese Merino sheep feeding standard is tested, by daily gain 50g/d ram hogg(et)s digestible energy (DE), slightly
1.5 times of protein (CP) recommended amounts prepare (table 1).Analyze pure tannic acid (tannic acid) limited purchased from the triumphant logical chemical reagent in Tianjin
Company.
The composition and trophic level of the experiment diet of table 1
Premix is as follows to the element percentage that every kilogram of daily ration is provided：Zn 60, Fe 60, Mn40, Cu 12, I 1, Se
0.2, Co 0.3.
Install before rumen fistula, experiment sheep is immunized, with keeping sheep hurdle and apparatus to clean during experiment before experiment, and
Periodically sterilization.Will experiment sheep be placed in individual digestion and metabolism cage raise, experiment the last week gradually transition for experiment diet, fine fodder and
After coarse fodder is crushed, it is prepared into TRM by Diet Formula and is fed, day feeding volume is about the 3% (average of body weight
1.0kg DM), point 3 equivalent feedings (07:00、14:00、21:00), free water.
1.3 sample collections and assay method
B, digestion and metabolism sample
Collect full excrement and full urine carries out digest supersession test, the phase of collecting is 5 days, fecaluria discharge rate is collected day by day and feeding is observed
Expect after being mixed, dried and crushed after inventory and surplus doses, full collecting dung, observation dry, organic substance and fiber
The digestion situation of material, and monitoring record calcium Ca, phosphorus P, nitrogen N content；Dried after full excrement is mixed in the step B at 65 DEG C
To constant weight, the H of mass concentration 10% is added2SO4Solution submerges excrement sample, is refrigerated in the case where 20 DEG C of ﹣, and H is added in full urine2SO4Solution,
Full urine ph values are reduced to less than 3, are refrigerated at 4 DEG C, and detection records calcium Ca, phosphorus P, nitrogen N content in full excrement and full urine；
C, rumen fluid sample
At times collect rumen fluid sample, respectively before food 1h, food after 1h, food after 3h, food after 5h, food after 7h from abdomen capsule
Rumen fluid 50mL is extracted, with pH value is determined, with 4 layers of filtered through gauze, filtrate is collected, filtrate adds saturation HgCl2Solution, in ﹣ 20
DEG C preserve, detection rumen fluid in total nitrogen, ammonia nitrogen NH3- N content；
D, plasma sample
2h after on the feed, gathers sheep jugular vein blood 10mL, adds heparin and shakes up, with 5000r/min centrifugal treating 10min systems
Blood plasma is taken, the urea nitrogen content in 20 DEG C of preservations of ﹣, detection blood plasma.
2 results and analysis
The influence that tannin acid content is utilized to sheep Nutrients Digestion and nitrogen in 2.1 diets
2.1.1 the apparent digestibility of dry, organic substance and fibrous matter
Table 2 shows that DM the and OM feed intakes of A, B, C processing, digestion amount pole are significantly higher than D (P ﹤ 0.01)；Each processing DM with
Discharge rate, digestibility difference is not notable (P ﹥ 0.05) in OM excrement, but DM digestibilities, the OM digestibilities of A, B, C processing are presented
Trend higher than D.It is also seen that A, B NDF the and ADF amounts of eating, digestion amount pole are significantly higher than C, D (P ﹤ 0.01), C NDF with
Also pole is significantly higher than D (P ﹤ 0.01) for the ADF amounts of eating, digestion amount；NDF and ADF excrement discharge rate, digestibility difference is not between processing
Significantly (P ﹥ 0.05).
Table 2 handles the influence to the apparent digestion of DM, OM, NDF and ADF
Same column of figure shoulder indicates different capitalizations and represents that difference extremely significantly (P ﹤ 0.01), there is different lowercase letters
Significant difference (P ﹤ 0.05), has identical big or lowercase letter indication difference not notable (P ﹥ 0.05), as follows
2.1.2 the apparent digestion of nitrogen and retain
Table 3 shows that the N amounts of eating and N digestion amounts pole for handling A, B, C are significantly higher than D (P ﹤ 0.01)；N discharges are urinated between processing
Difference is not notable (P ﹥ 0.05) between amount and N digestibility；Excrement N discharge rates decline with the increase of tannic acid ratio, and processing A is notable
Higher than processing C, D, processing B is significantly higher than processing D；Processing C N amount retaineds are significantly higher than A, D (P ﹤ 0.01)；N Retentions are with list
Rather the increase of acid ratio is in the trend risen, and only C processing is significantly higher than A (P ﹤ 0.01), not notable (the P ﹥ of the difference between other processing
0.05)。
The digestion apparent to N of the test process of table 3 and the influence retained
2.1.3 the apparent digestion of calcium, phosphorus
Found out by table 4, the Ca amounts of eating and B differences for handling A be not notable, but is significantly higher than C, D (P ﹤ 0.01)；C Ca foods
Entering amount, also pole is significantly higher than D (P ﹤ 0.01)；Processing A, B, C excrement Ca discharge rates pole are significantly higher than D (P ﹤ 0.01)；Between each treatment group
Ca digestion amount, digestibility are without significant difference (P ﹥ 0.05).Processing A, B, C the P amounts of eating pole are significantly higher than D (P ﹤ 0.01), everywhere
P excrement discharge rate, digestion amount, the difference of digestibility is not notable (P ﹥ 0.05) between reason group.
Influence of the test process of table 4 to the apparent digestion of Ca, P
Influence of the tannin acid content to rumen metabolic parameters in 2.2 feeds
Found out by table 5, each processing tannic acid content does not make significant difference (P ﹥ 0.05) to rumen fluid ph and total N content average；And
With the increase of tannic acid, rumen fluid NH3- N averages and plasma urea nitrogen linearly decline, A NH3- N is significantly higher than D (P ﹤
0.05), and its blood plasma BUN be higher than C, D (P ﹤ 0.01).
The cud nitrogen metabolism parameter of table 5 and plasma urea nitrogen
3 discuss
The influence that 3.1 diet tannic acid levels digest to sheep DM, OM and fibrous matter
Low concentration tannin acid content does not influence the feed intake of sheep, concentration of tannin acid (20gkg-1DM), hence it is evident that influence
The palatability of diet, so that the feed intake of sheep is reduced, and then influence sheep is to DM, OM, NDF and ADF intake.With list
The increase of peaceful acid concentration, sheep is to DM, OM, NDF and ADF digestibility and does not make significant difference, and research shows, castrated ram's goat feeding is rich
Feed containing tannin, compared with the feed without tannin of searching for food, its DM, OM, NDF and ADF digestibility is all significantly improved.
Influence of the 3.2 diet tannic acid levels to nitrogen metabolism parameter and digestibility and utilization
Tannic acid can be combined into tannin-protein complex in cud with protein, as rumen bypass protein, work as arrival
Compound can release albumen after abomasum and small intestine, so add absorption of the small intestine to amino acid, or add the ammonia of cud
Base acid outflow rate；It is further proposed that can prevent deamination in ruminal soluble protein and tannin combination, fresh herbage contains
Substantial amounts of soluble protein, degrades fast, tannin can effectively prevent the fast degradation of protein, objectively add after feeding
The quantity of ruminal protein.There is tannic acid (Hydrolysable Tannins) protected protein matter to exempt from ruminal degradation, improve the effect of nitrogen retention.Instead
After the low forage grass of hay feed intake tannin content, degradation rate of the soluble protein in cud is reduced, makes rumen bypass protein
Quantity increase, so as to improve the efficiency that domestic animal utilizes protein.The study show that, in tannic acid low concentration, with diet list
The increase of peaceful acid concentration, the amount retained of nitrogen, Retention are significantly improved；The reduction of CP degradation rates, causes in cud in rumen fluid
NH3The decline of-N concentration, variation tendency and the rumen fluid NH of plasma urea nitrogen3- N is consistent, and the two has very high correlation
(r=0.999, P=0.001), shows plasma urea nitrogen mainly by rumen fluid NH3- N is restricted.Diet tannin acid concentration is mainly led to
The Retention for reducing urinary nitrogen discharge rate and improving nitrogen is crossed, fecal nitrogen discharge rate is substantially uninfluenced.
The principal element that act on protein of influence tannic acid is the binding ability of tannic acid and protein, Nitrogen digestibility and
Tannin is in inverse ratio to protein binding capacity.The bond strength of tannin-protein complex depends on the spy of tannin and protein
Property, i.e. the compatibility of molecular weight, tertiary structure, isoelectric point and binding site, tannin level is 3.40gkg-1The urine of DM diets
Nitrogen output is substantially less than other treatment groups, and nitrogen retention ratio pole is significantly higher than other each treatment groups.
3.3 diet tannic acid levels are to calcium, the influence of phosphorus digestion
The study show that, Ca, P digestion amount and digestibility difference is not notable between each treatment group.Tannin reduces sulphur in cud
Degraded and absorption, improve digesting and assimilating for phosphorus and zinc, and the influence of digesting and assimilating on sodium, calcium, magnesium, iron, manganese etc. does not show
Write[22].The tannic acid such as Loque (Hydrolysable Tannins) and the calcium binding outside intracellular or tissue, can antagonism smooth muscle and cardiac muscle
Calcium induction is shunk, and is reduced blood pressure[23].And digest and assimilate apparent to Ca, P of tannin acid concentration does not make significant difference yet in this experiment, base
This is consistent with forefathers' report.
4 conclusions
The study show that, tannic acid has effects that to improve the digested absorption of aleuroplast；Tannin acid content is small in diet
In 15gkg-1During DM, on sheep feeding influence very little, and significantly improve nitrogen retention ratio, the effect absorbed to protein digestibility compared with
It is good；And it is 15~20gkg to work as tannin acid content in diet-1During DM, the feed intake of sheep is significantly affected；Therefore tannic acid is in diet
In addition should not exceed 15gkg-1DM.At present, protein feed resource is relative lacks, and the high feed of tannin content is former
Material can be reasonably combined with other feeds, to improve the biological value of feed nutrition material, it is also contemplated that adding in right amount
Tannic acid is used as feed addictive.
Claims (6)
1. a kind of sheep tannin acid type feed formula, it is characterised in that including basal feed and tannic acid, admixed in basal feed
Tannic acid, tannic acid addition is 0.1-20gkg-1, it is more obtained than dispensing that basal feed presses following weight：It is 35-40 parts of corn, big
5-10 parts of dregs of beans, 10-15 parts of alfalfa hay, 25-30 parts of maize straw, 15-20 parts of Barley straw, 0.1-0.2 parts of calcium monohydrogen phosphate,
0.1-0.2 parts of 0.3-0.4 parts of agstone, 0.5-1 parts of salt and premix.
2. a kind of sheep tannin acid type feed formula according to claim 1, it is characterised in that the basal feed is by such as
Lower weight is made than dispensing：37.85 parts of corn, 6 parts of Soybean Meal, 12 parts of alfalfa hay, 27 parts of maize straw, Barley straw 16
Part, 0.17 part of calcium monohydrogen phosphate, 0.33 part of agstone, 0.5 part of salt and 0.15 part of premix.
3. a kind of sheep tannin acid type feed formula according to claim 2, it is characterised in that by such as in the premix
Lower weight is made than trace element：60 parts of zinc Zn, 60 parts of iron Fe, Mn40 parts of manganese, 12 parts of copper Cu, 1 part of iodine I, selenium Se 0.2
Part, 0.3 part of cobalt Co.
4. a kind of sheep tannin acid type feed formula according to claim 1, it is characterised in that the tannic acid addition
For 3.40gkg-1。
5. according to a kind of any one of Claims 1-4 digestion and metabolism assay method of sheep tannin acid type feed formula, its
It is characterised by comprising the following steps：
A, early-stage preparations
Rumen fistula, conventional feed and sheep tannin acid type feed mixing feeding are installed to experiment sheep, and gradually increase sheep is single
Peaceful acid type feed dosage, until complete feeding sheep tannin acid type feed, experiment sheep feeding sheep tannin acid type feed formula one
Start sampling after week, day feeding volume is the 3% of body weight, and experiment sheep, which is freely fetched water, drinks；
B, digestion and metabolism sample
Collect full excrement and full urine carries out digest supersession test, the phase of collecting is 5 days, fecaluria discharge rate is collected day by day and feed throwing is observed
After being mixed, dried and crushed after doses and surplus doses, full collecting dung, observation dry, organic substance and fibrous matter
Digestion situation, and monitoring record calcium Ca, phosphorus P, nitrogen N content；
C, rumen fluid sample
Rumen fluid sample is collected at times, and 7h is extracted from abdomen capsule after 5h, food after 3h, food after 1h, food after 1h, food before food respectively
Rumen fluid 50mL, with pH value is determined, with 4 layers of filtered through gauze, collects filtrate, filtrate adds saturation HgCl2Solution, in 20 DEG C of guarantors of ﹣
Deposit, total nitrogen, ammonia nitrogen NH in detection rumen fluid3- N content；
D, plasma sample
2h after on the feed, gathers sheep jugular vein blood 10mL, adds heparin and shakes up, blood is produced with 5000r/min centrifugal treatings 10min
Slurry, the urea nitrogen content in 20 DEG C of preservations of ﹣, detection blood plasma.
6. a kind of digestion and metabolism assay method of sheep tannin acid type feed formula according to claim 5, it is characterised in that
Full excrement dries the H that mass concentration 10% is added to constant weight at 65 DEG C in the step B after mixing2SO4Solution submerges excrement sample,
Refrigerated in the case where 20 DEG C of ﹣, H is added in full urine2SO4Solution, full urine ph values are reduced to less than 3, are refrigerated at 4 DEG C, and detection record is complete
Calcium Ca, phosphorus P, nitrogen N content in excrement and full urine.
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Cited By (2)
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| CN108354078A (en) * | 2018-02-12 | 2018-08-03 | 中国农业科学院北京畜牧兽医研究所 | The preparation method of the compound Supplementation In Diet feed of forage grass type |
| CN109349221A (en) * | 2018-11-16 | 2019-02-19 | 西藏自治区农牧科学院畜牧兽医研究所 | A kind of method of the energy and protein requirement of determining growth period Tibet White pile goat |
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