CN113318150A - Application of macleaya cordata extract in preparation of oral liquid for promoting animal growth - Google Patents
Application of macleaya cordata extract in preparation of oral liquid for promoting animal growth Download PDFInfo
- Publication number
- CN113318150A CN113318150A CN202110425623.3A CN202110425623A CN113318150A CN 113318150 A CN113318150 A CN 113318150A CN 202110425623 A CN202110425623 A CN 202110425623A CN 113318150 A CN113318150 A CN 113318150A
- Authority
- CN
- China
- Prior art keywords
- macleaya cordata
- extract
- oral liquid
- preparation
- macleaya
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/66—Papaveraceae (Poppy family), e.g. bloodroot
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/12—Carboxylic acids; Salts or anhydrides thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Diabetes (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Obesity (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the technical field of preparation of traditional Chinese veterinary medicines, and particularly relates to an application of a macleaya cordata extract in preparation of an oral liquid for promoting animal growth; the effective components of the fresh leaves and stems of the macleaya cordata and the macleaya cordata fruits are extracted in different modes, the whole plant of the macleaya cordata is fully utilized, the macleaya cordata leaf and stem extract and the macleaya cordata fruit extract which are respectively extracted are matched, and auxiliary materials are added to obtain the pure traditional Chinese medicine oral liquid without antibiotics, which is used for treating inappetence, dyspepsia, growth retardation and other diseases of cattle, sheep, pigs, poultry and the like, has obvious curative effect, can not cause the accumulation of antibiotics in livestock and poultry bodies, avoids the induction of drug resistance, has no toxic and side residual effect after being used, meets the medication requirements for producing pollution-free livestock products, organic livestock products and green livestock products, and is a better substitute-resistant traditional Chinese veterinary medicine product at present.
Description
Technical Field
The invention belongs to the technical field of preparation of traditional Chinese veterinary medicines, and particularly relates to an application of macleaya cordata extract in preparation of an oral liquid for promoting animal growth.
Background
For a long time, the safety and sanitation of animal food are seriously influenced by the residues of antibiotics and chemical veterinary drugs, and the healthy development of ecological animal husbandry is influenced. The global elimination and the limitation of the use varieties of chemical veterinary drugs and antibiotics are increasing. All growth-promoting drug feed additive varieties except the traditional Chinese medicines are withdrawn from 1 month and 1 day in 2020. The national veterinary medicine is a treasure left by Chinese nation in the long-term struggle with animal diseases, is a pure natural green animal health care product, has rich resources, is a new industry, has huge development space, market prospect and export earning potential, and is the research and development direction of veterinary medicines in China and in the current world.
Macleaya cordata, named as haocasiae, haocasiae pole, paulownia pole, Boletus, Haimazai, herba Menthae, Huang Po mother, Sanqian, fructus Cannabis and Huihuo tube, is a perennial big herb of macleaya genus of Papaveraceae, and has a long history in China for treating scabies, furunculosis, killing insects and maggots, etc. The whole herb has strong toxicity, and can be used for treating traumatic injury, arthritis, tinea versicolor, malignant boil, bee sting, anesthesia, pain relieving, and detumescence; it can be used as agricultural chemical for preventing and treating rice stink bug, rice bud worm, oncomelania, etc. Recent pharmacological research shows that the traditional Chinese medicine composition is effective in treating various inflammations. The sanguinarine and chelerythrine which are main effective components of macleaya cordata play an important role in the efficacy of the macleaya cordata, and the volatile oil in the leaves and stems of the macleaya cordata mainly contains nonadecane, butyl phthalate, n-pentadecanal and the like, has good free radical scavenging capacity, and shows good antioxidant activity.
Patent document with application number of CN201710863864.X discloses a compound macleaya cordata composition preparation for treating animal diarrhea diseases and application thereof, wherein the active ingredients of the composition comprise macleaya cordata total alkaloid extract and quinolone compounds, and the composition is used for treating animal diarrhea diseases. However, the quinolone compound is adopted for compatibility, the quinolone medicine has certain medicine safety risk, belongs to eliminated and limited veterinary antibiotics, and animal food with residual quinolone medicine after long-term eating by people can cause respiratory tract infection, gene mutation, carcinogenesis and the like of a human body, and easily induces the transmission of drug resistance, so that the clinical curative effect of the medicine and the safety of the animal food are influenced. No. 194 bulletin of rural agricultural departments indicates that all varieties of growth-promoting drug feed additives except traditional Chinese medicines are withdrawn from 2020 and 1 month and 1 day.
Disclosure of Invention
The invention provides an application of macleaya cordata extract in preparation of oral liquid for promoting animal growth in order to solve the problems.
The method is realized by the following technical scheme:
1. an oral liquid for promoting animal growth is prepared from herba Macleayae Cordatae extract as main ingredient, and cosolvent 0.5-1 wt%, correctant 5 wt%, and antiseptic 0.05 wt%.
Furthermore, the macleaya cordata extract is prepared by mixing macleaya cordata leaf and stem extract which is equal to or more than 0.5g of the primary medicine of the macleaya cordata leaf and stem and the macleaya cordata fruit extract which is equal to or more than 5mg of alkaloid per milliliter.
Further, the cosolvent is polysorbate-80.
Further, the flavoring agent is glucose.
Further, the preservative is sorbic acid.
2. The preparation method of the macleaya cordata extract comprises the following steps:
(1) preparing a macleaya cordata leaf and stem extract:
cutting leaf and stem of herba Macleayae Cordatae, sieving with 40 mesh sieve, adding supercritical CO2In an extraction tank, extracting at 50 deg.C under 25MPa with 95% ethanol as entrainer for 1 hr to obtain Macleaya cordata extractive solution (per ml equivalent to 1g of crude drug), and refrigerating.
(2) Preparing a macleaya cordata fruit extracting solution:
(a) boiling and crushing in boiling water: placing herba Macleayae Cordatae coarse powder into a reduced pressure reaction kettle, adding equivalent amount of boiling water, and soaking for 30-60min under boiling state;
(b) glycollic acid digestion and heat extraction: extracting 95% acid ethanol at 60-80 deg.C for 1-2 hr, wherein the amount of ethanol is 3-5 times of total amount of the Macleaya cordata coarse powder-boiling water mixture;
(c) vacuum concentration and filtration: filtering the extractive solution, recovering ethanol under reduced pressure until the ethanol content of the extractive solution is 30%, and filtering while hot;
(d) removing impurities by using a cold and hot treatment clarifying agent: adding clarifier 0.1-0.2% of the filtrate into the filtrate, stirring, standing at 5-15 deg.C for 12 hr, filtering, adjusting pH to 3-5, heating, and adding distilled water until the total alkaloid content per ml is not less than 10 mg.
Further, the boiling water in the step (1) is acid water with pH adjusted to 2-3 by sulfuric acid.
(3) Preparing the macleaya cordata extract: and mixing the extract of the leaves and stems of the macleaya cordata and the extract of the macleaya cordata fruits uniformly to obtain the macleaya cordata extract.
3. The preparation method of the oral liquid for promoting the growth of animals comprises the following steps: diluting herba Macleayae Cordatae extract with distilled water to obtain diluent, adjusting pH to 4-6, adding cosolvent, correctant and antiseptic, stirring, filtering, quality testing, quantitatively bottling, sealing, and packaging.
Furthermore, the content of total alkaloids in each milliliter of diluent is more than or equal to 5mg, and each milliliter of diluent is equivalent to that of leaf and stem protogenic drugs of the fresh plant macleaya cordata is more than or equal to 0.5 g.
In conclusion, the beneficial effects of the invention are as follows: the invention extracts effective components from the fresh leaves and stems of macleaya cordata and macleaya cordata fruits in different modes, not only fully utilizes the whole plant of macleaya cordata, but also mixes the macleaya cordata leaf and stem extract and macleaya cordata fruit extract obtained by respective extraction, adds auxiliary materials to obtain the pure traditional Chinese medicine oral liquid without antibiotics, is used for treating diseases such as inappetence, dyspepsia, growth retardation and the like of cattle, sheep, pigs, poultry and the like, has obvious curative effect, cannot cause the accumulation of antibiotics in livestock and poultry bodies, avoids the transfer of induced drug resistance, has no toxic or side residual effect after use, and meets the medication requirements for producing pollution-free livestock products and green livestock products.
Wherein, the pharmacological actions are as follows: macleaya cordata, bitter and cold in flavor. It is toxic. The main treatment is as follows: dispel wind, remove toxicity, dissipate blood stasis and relieve swelling. Can be used for treating traumatic injury, rheumatic arthralgia, carbuncle, furuncle, toxic swelling, and ulcer of lower limbThe product is pounded and applied topically or ground into powder and applied to the affected part), vaginal infusorian (vagina is washed by decoction), eczema (appearance of decoction), and burn and scald (affected part is ground into powder and applied). And can kill maggots. The method adopts the leaves and stems of the fresh macleaya cordata to extract the volatile oil, has high content and good quality of the volatile oil, fully utilizes the whole plant, does not cause waste, and is more economical and practical than the method of extracting the macleaya cordata fruits only. The essential oil in the stems and leaves of Macleaya cordata mainly comprises nonadecane, butyl phthalate, n-pentadecanal and the like, and has good capacity of removing free radicals. By using supercritical CO2The macleaya cordata extract is prepared by extracting volatile oil from the leaves and stems of macleaya cordata, boiling in boiling water, crushing and solubilizing, glycollic acid digestion by heating, extracting, vacuum concentrating and filtering, precipitating and removing impurities by using a clarifying agent, and mixing the extract with the extract and refining by a new process and a new technology, does not need a drying process, effectively reduces the production cost and saves about 30 percent of manpower and material resources. The finished product has high content of effective components, stable quality and good color, is suitable for industrial production, and is convenient to package, transport and carry. The macleaya cordata alkaloid and the macleaya cordata volatile oil are compatible, so that the effect of the whole effective components of the macleaya cordata can be obvious, the macleaya cordata alkaloid and the macleaya cordata volatile oil have good synergistic effect, the drug effect can be enhanced, and the effect that 1+1 is more than 2 is achieved. The veterinary drug has obvious curative effect on inappetence, dyspepsia, growth retardation and other diseases of cattle, sheep, pigs (particularly piglets), poultry and the like, and is superior to other similar veterinary drugs. The oral liquid can be directly absorbed by the stomach and the intestine more quickly and completely through oral administration, has high bioavailability and better drug effect, and can better exert the traditional characteristic advantages of the overall effect of multiple effects, bidirectionality, cooperativity and regulation of the treatment effect. And embodies the advantages of the modern scientific technology in refining Chinese herbal medicines, convenient and quick use, easy carrying and the like. Accords with the development direction of 'green products', and is a better 'substitute for antibiotics' traditional Chinese veterinary medicine product. The traditional Chinese veterinary medicine oral liquid product prepared by extracting the effective components of the pure natural green plants has no toxic or side effect after use, is safe and reliable, convenient to use, wide in application range, remarkable in curative effect and quick in effect, and meets the medication requirements of producing pollution-free animal products and green animal products.
Detailed Description
The following is a detailed description of the embodiments of the present invention, but the present invention is not limited to these embodiments, and any modifications or substitutions in the basic spirit of the embodiments are included in the scope of the present invention as claimed in the claims.
Example 1
1. An oral liquid for promoting animal growth is prepared from Macleaya cordata extract as main ingredient, and 0.5% of polysorbate-80, 5% of glucose and 0.05% of sorbic acid by weight.
Furthermore, the macleaya cordata extract is prepared by mixing macleaya cordata leaf and stem extract which is equal to or more than 0.5g of the primary medicine of the macleaya cordata leaf and stem and the macleaya cordata fruit extract which is equal to or more than 5mg of alkaloid per milliliter.
2. The preparation method of the macleaya cordata extract comprises the following steps:
(1) preparing a macleaya cordata leaf and stem extract:
5000 g of fresh plant macleaya cordata leaves and stems are cut into pieces and sieved by a 40-mesh sieve, and supercritical CO is added into the pieces2In an extraction tank, extracting at 50 deg.C under 25MPa with 95% ethanol as entrainer for 1 hr to obtain Macleaya cordata extractive solution (per ml equivalent to 1g of crude drug), and refrigerating.
(2) Preparing a macleaya cordata fruit extracting solution:
(a) boiling and crushing in boiling water: putting 5000 g of macleaya cordata fruit coarse powder into a reduced pressure reaction kettle, adding boiling water with the same amount, and soaking for 50min under the boiling state;
(b) glycollic acid digestion and heat extraction: extracting 95% acid ethanol at 70 deg.C for 2 hr, wherein the amount of ethanol is 4 times of total amount of the macleaya cordata coarse powder-boiling water mixture;
(c) vacuum concentration and filtration: filtering the extractive solution, recovering ethanol under reduced pressure until the ethanol content of the extractive solution is 30%, and filtering while hot;
(d) removing impurities by using a cold and hot treatment clarifying agent: adding 0.2% volume of clarifier into the filtrate, stirring, standing at 5 deg.C for 12 hr, filtering, adjusting pH to 4, heating, and adding 5000ml distilled water to obtain Macleaya cordata fruit extract with total alkaloid content of not less than 10mg per ml.
Further, the boiling water in the step (1) is acid water adjusted to pH 3 with sulfuric acid.
(3) Preparing the macleaya cordata extract: and mixing the extract of the leaves and stems of the macleaya cordata and the extract of the macleaya cordata fruits uniformly to obtain the macleaya cordata extract.
3. The preparation method of the oral liquid for promoting the growth of animals comprises the following steps: adding distilled water to 10000ml to obtain diluted solution, adjusting pH to 5, adding cosolvent, correctant and antiseptic, stirring well, filtering, quality testing, quantitatively bottling, sealing, and packaging.
Furthermore, the content of total alkaloids in each milliliter of diluent is more than or equal to 5mg, and each milliliter of diluent is equivalent to that of leaf and stem protogenic drugs of the fresh plant macleaya cordata is more than or equal to 0.5 g.
Example 2
1. An oral liquid for promoting animal growth is prepared from Macleaya cordata extract as main ingredient, polysorbate-80 (1 wt%), glucose (5 wt%) and sorbic acid (0.05 wt%).
Furthermore, the macleaya cordata extract is prepared by mixing macleaya cordata leaf and stem extract which is equal to or more than 0.5g of the primary medicine of the macleaya cordata leaf and stem and the macleaya cordata fruit extract which is equal to or more than 5mg of alkaloid per milliliter.
2. The preparation method of the macleaya cordata extract comprises the following steps:
(1) preparing a macleaya cordata leaf and stem extract:
5000 g of fresh plant macleaya cordata leaves and stems are cut into pieces and sieved by a 40-mesh sieve, and supercritical CO is added into the pieces2In an extraction tank, extracting at 60 deg.C under 20MPa with 95% ethanol as entrainer for 1 hr to obtain Macleaya cordata extractive solution (per ml equivalent to 1g of crude drug), and refrigerating.
(2) Preparing a macleaya cordata fruit extracting solution:
(a) boiling and crushing in boiling water: taking 5000 g of macleaya cordata fruit coarse powder, putting the macleaya cordata fruit coarse powder into a pressure reduction reaction kettle, adding boiling water with the same amount, and soaking for 30 mm under the boiling state;
(b) glycollic acid digestion and heat extraction: extracting 95% acid ethanol at 60 deg.C for 2 hr, wherein the amount of ethanol is 3 times of total amount of the macleaya cordata coarse powder-boiling water mixture;
(c) vacuum concentration and filtration: filtering the extractive solution, recovering ethanol under reduced pressure until the ethanol content of the extractive solution is 30%, and filtering while hot;
(d) removing impurities by using a cold and hot treatment clarifying agent: adding 0.1% volume of clarifier into the filtrate, stirring, standing at 8 deg.C for 12 hr, filtering, adjusting pH to 6, heating, and adding 5000ml distilled water to obtain Macleaya cordata fruit extract with total alkaloid content of more than 10mg per ml.
Further, the boiling water in the step (1) is acid water adjusted to pH 2 with sulfuric acid.
(3) Preparing the macleaya cordata extract: and mixing the extract of the leaves and stems of the macleaya cordata and the extract of the macleaya cordata fruits uniformly to obtain the macleaya cordata extract.
3. The preparation method of the oral liquid for promoting the growth of animals comprises the following steps: adding distilled water to 10000ml to obtain diluted solution, adjusting pH to 6, adding cosolvent, correctant and antiseptic, stirring well, filtering, quality testing, quantitatively bottling, sealing, and packaging.
Furthermore, the content of total alkaloids in each milliliter of diluent is more than or equal to 6mg, and each milliliter of diluent is equivalent to that of leaf and stem protogenic drugs of the fresh plant macleaya cordata and is more than or equal to 0.4 g.
Example 3
1. An oral liquid for promoting animal growth is prepared from Macleaya cordata extract as main ingredient, and polysorbate-80, 5% glucose and 0.05% sorbic acid in an amount of 0.7% by weight of the Macleaya cordata extract.
Furthermore, the macleaya cordata extract is prepared by mixing macleaya cordata leaf and stem extract which is equal to or more than 0.5g of the primary medicine of the macleaya cordata leaf and stem and the macleaya cordata fruit extract which is equal to or more than 5mg of alkaloid per milliliter.
2. The preparation method of the macleaya cordata extract comprises the following steps:
(1) preparing a macleaya cordata leaf and stem extract:
putting into supercritical CO2In an extraction tank, extracting at 55 ℃ under 22MPa with 95% ethanol as entrainer for 1 hour to obtain macleaya cordata extractive solution, wherein each ml of the extractive solution is equal to 1g of the crude drug, and refrigerating for later use.
(2) Preparing a macleaya cordata fruit extracting solution:
(a) boiling and crushing in boiling water: taking 5000 g of macleaya cordata fruit coarse powder, putting the macleaya cordata fruit coarse powder into a pressure reduction reaction kettle, adding boiling water with the same amount, and soaking for 60 mm under the boiling state;
(b) glycollic acid digestion and heat extraction: extracting 95% acid ethanol at 50 deg.C for 1.5 hr, wherein the amount of ethanol is 5 times of total amount of the Macleaya cordata coarse powder-boiling water mixture;
(c) vacuum concentration and filtration: filtering the extractive solution, recovering ethanol under reduced pressure until the ethanol content of the extractive solution is 30%, and filtering while hot;
(d) removing impurities by using a cold and hot treatment clarifying agent: adding 0.2% volume of clarifier into the filtrate, stirring, standing at 12 deg.C for 12 hr, filtering, adjusting pH to 5, heating, and adding 5000ml distilled water to obtain Macleaya cordata fruit extract with total alkaloid content of more than 10mg per ml.
Further, the boiling water in the step (1) is acid water adjusted to pH 3 with sulfuric acid.
(3) Preparing the macleaya cordata extract: and mixing the extract of the leaves and stems of the macleaya cordata and the extract of the macleaya cordata fruits uniformly to obtain the macleaya cordata extract.
3. The preparation method of the oral liquid for promoting the growth of animals comprises the following steps: adding distilled water to 10000ml to obtain diluted solution, adjusting pH to 6, adding cosolvent, correctant and antiseptic, stirring well, filtering, quality testing, quantitatively bottling, sealing, and packaging.
Furthermore, the content of total alkaloids in each milliliter of diluent is more than or equal to 6mg, and each milliliter of diluent is equivalent to that of leaf and stem protogenic drugs of the fresh plant macleaya cordata and is more than or equal to 0.5 g.
Example 4
1. An oral liquid for promoting animal growth is prepared from Macleaya cordata extract as main ingredient, and polysorbate-80, 5% glucose and 0.05% sorbic acid in an amount of 0.8% by weight of the Macleaya cordata extract.
Furthermore, the macleaya cordata extract is prepared by mixing macleaya cordata leaf and stem extract which is equal to or more than 0.5g of the primary medicine of the macleaya cordata leaf and stem and the macleaya cordata fruit extract which is equal to or more than 5mg of alkaloid per milliliter.
2. The preparation method of the macleaya cordata extract comprises the following steps:
(1) preparing a macleaya cordata leaf and stem extract:
5000 g of fresh plant macleaya cordata leaves and stems are cut up and sieved by a 40-mesh sieve, the cut leaves and stems are put into a distiller to be distilled by water separation, primary distilled liquid is collected and put into the distiller to be distilled again, and secondary distilled liquid with the volume per milliliter equal to 1g of the original medicine is obtained and is refrigerated for standby.
(2) Preparing a macleaya cordata fruit extracting solution:
(a) boiling and crushing in boiling water: taking 5000 g of macleaya cordata fruit coarse powder, putting the macleaya cordata fruit coarse powder into a reduced pressure reaction kettle, adding boiling water with the same amount, and soaking for 50 mm under the boiling state;
(b) glycollic acid digestion and heat extraction: extracting 95% acid ethanol at 60 deg.C for 2 hr, wherein the amount of ethanol is 5 times of total amount of the macleaya cordata coarse powder-boiling water mixture;
(c) vacuum concentration and filtration: filtering the extractive solution, recovering ethanol under reduced pressure until the ethanol content of the extractive solution is 30%, and filtering while hot;
(d) removing impurities by using a cold and hot treatment clarifying agent: adding 0.2% volume of clarifier into the filtrate, stirring, standing at 10 deg.C for 12 hr, filtering, adjusting pH to 5, heating, and adding 5000ml distilled water to obtain Macleaya cordata fruit extract with total alkaloid content of more than 10mg per ml.
Further, the boiling water in the step (1) is acid water adjusted to pH 3 with sulfuric acid.
(3) Preparing the macleaya cordata extract: and mixing the extract of the leaves and stems of the macleaya cordata and the extract of the macleaya cordata fruits uniformly to obtain the macleaya cordata extract.
3. The preparation method of the oral liquid for promoting the growth of animals comprises the following steps: adding distilled water to 10000ml to obtain diluted solution, adjusting pH to 6, adding cosolvent, correctant and antiseptic, stirring well, filtering, quality testing, quantitatively bottling, sealing, and packaging.
Furthermore, the content of total alkaloids in each milliliter of diluent is more than or equal to 6mg, and each milliliter of diluent is equivalent to that of leaf and stem protogenic drugs of the fresh plant macleaya cordata and is more than or equal to 0.5 g.
The clarifying agents used in the above examples are all art-accepted clarifying agents.
Test for promoting growth of broiler chickens
1.1 Experimental methods
Selecting 90 healthy three-yellow hybrid broilers with the age of 10 days and similar body weight, and randomly dividing the broilers into 3 groups: the control group, macleaya cordata alkali group and macleaya cordata oleine group were each prepared by repeating 3 times each of 10 chickens. The test period was 30 days. The three-layer stacked metal cages are adopted for cage culture, and each repeated test chicken is bred in the same cage. Wherein the control group is fed with basal diet, the macleaya cordata alkali group is fed with basal diet +10 ml/group of macleaya cordata alkali oral liquid, and the macleaya cordata oleyl alkali group is fed with basal diet +10 ml/group of macleaya cordata oleyl alkali oral liquid. According to conventional feeding management, the feeding environment and the feeding management mode of each group are consistent, free feeding and free drinking water are realized in the whole period, the cleanness and sanitation in the chicken house are kept, good ventilation is realized, the chicken house is disinfected regularly, and the macleaya cordata alkali oral liquid are added into the drinking water to enable the chicken house to feed freely.
1.2 Experimental indices
The initial weight of the test chicken is weighed on the day of the beginning of the normal test period (10 days old), the test chicken is weighed on an empty stomach in the morning of 15d and 30d after the test is started, and the total weight and the average weight of the weight gain of each period of each repetition are recorded.
1.3 results of the experiment
The effect of the oral liquid on the weight of the three-yellow hybrid broiler is shown in table 1.
TABLE 1
According to the experimental results, compared with a control group, the daily gain weight of the bred three-yellow hybrid broiler chickens of 10-30 days old is improved, and the average weight gain of the macleaya cordata alkali oral liquid group is improved by 271g compared with the control group at 20 days; the average weight gain of the macleaya cordata oleifera oral liquid group is increased by 406g compared with that of a control group, and the average weight gain of the macleaya cordata oleifera oral liquid group is increased by 135g compared with that of the macleaya cordata oleifera oral liquid group; the average weight gain of the macleaya cordata alkali oral liquid group is increased by 233g compared with that of the control group at 30d, the average weight gain of the macleaya cordata oleyl alkali oral liquid group is increased by 370g compared with that of the control group, and the average weight gain of the macleaya cordata alkali oral liquid group is increased by 137g compared with that of the macleaya cordata alkali oral liquid group.
Second, acute toxicity test
1. Material
1.1 Experimental animals: 20 clean-grade healthy Wistar rats weighing 180-.
1.2 test drugs: the macleaya cordata growth promoting oral liquid prepared by the method of example 1 is a test liquid.
2. Experimental methods
According to the 'guiding principle of veterinary drug acute toxicity test', 20 clean-grade healthy Wistar rats are taken and separately raised (5 rats per cage), after fasting for 12 hours, the Wistar rats are respectively weighed, numbered and orally administered, and the LD of the macleaya cordata oral liquid for orally infecting the Wistar rats is measured by adopting a maximum dose method50The contamination was stopped when the oral contamination dose of the rats reached or exceeded 5g/kg b.w. When the contamination dose of the pre-experimental rats reaches more than 5g/kg b.w, the rats all survive. According to the maximum contamination dose of 5g/kg b.w of preliminary experiment, contamination is carried out, and LD is determined50The observation continued for 14 days after the end of the last infection, and the rats were observed and recorded for clinical symptoms and death. During the observation period rats had free access to food and water. At the end of the observation period all surviving rats were sacrificed and examined for ocular pathology.
3. Results of the experiment
All rats in the observation period showed normal clinical manifestations, no obvious neurological symptoms, no abnormal skin fur, normal food intake, drinking water, defecation and the like, and 20 rats survived by the end of the observation period. No obvious pathological changes were observed in the eye when the live rats were examined by dissection. The result shows that the macleaya cordata oral liquid is toxic LD for rats through mouth50More than 5g/kg b.w, is very safe.
The experiments show that the oral liquid obtained by matching the macleaya cordata leaf and stem extract and the macleaya cordata fruit extract has the advantages of synergistic effect, obvious growth promoting effect, no toxic or side residue after use, safety, reliability, convenient use, wide application range, obvious curative effect and quick response, and meets the medication requirements for producing pollution-free animal products and green animal products.
Claims (9)
1. An application method of macleaya cordata extract in preparing an oral liquid for promoting animal growth is characterized in that the oral liquid comprises the following components: macleaya cordata extract, cosolvent, flavoring agent and preservative; wherein, the dosage of the cosolvent is 0.5-1% of the mass of the oral liquid, the flavoring agent is 5% of the mass of the oral liquid, and the preservative is 0.05% of the mass of the oral liquid.
2. The use of macleaya cordata extract in the preparation of an oral liquid for promoting animal growth according to claim 1, wherein the macleaya cordata extract is prepared by mixing macleaya cordata leaf and stem extract with the amount of more than or equal to 0.5g of the primary drug of the leaf and stem of macleaya cordata and macleaya cordata fruit extract with the amount of more than or equal to 5mg of alkaloid per ml.
3. The use of macleaya cordata extract in the preparation of an oral liquid for promoting animal growth as claimed in claim 2, wherein the extract of the stems and leaves of macleaya cordata is obtained by subjecting the stems and leaves of fresh macleaya cordata to supercritical CO extraction2The preparation method comprises the following steps of.
4. The use of macleaya cordata extract in the preparation of an oral liquid for promoting animal growth as claimed in claim 2, wherein the supercritical CO is present2The extraction method adopts 50 ℃ of extraction temperature, 25MPa of extraction pressure, 95 percent ethanol as entrainer and 1h of extraction time.
5. The use of macleaya cordata extract in the preparation of an oral liquid for promoting animal growth as claimed in claim 2, wherein the macleaya cordata extract is prepared by the steps of:
(1) boiling and crushing in boiling water: adding equal amount of boiling water into the coarse powder of Macleaya cordata fruit, and soaking for 30-60mim under boiling state;
(2) glycollic acid digestion and heat extraction: leaching 95% acid ethanol at 60-80 deg.C for 1-2 hr;
(3) vacuum concentration and filtration: filtering the extractive solution, recovering ethanol under reduced pressure until the ethanol content of the extractive solution is 30%, and filtering while hot;
(4) removing impurities by using a cold and hot treatment clarifying agent: adding clarifier 0.1-0.2% of the filtrate into the filtrate, stirring, standing at 5-15 deg.C for 12 hr, filtering, adjusting pH to 3-5, heating, and adding distilled water until the total alkaloid content per ml is not less than 10 mg.
6. The use of macleaya cordata extract in the preparation of an oral liquid for promoting animal growth as claimed in claim 5, wherein the boiling water is acid water adjusted to pH 2-3 with sulfuric acid.
7. An oral liquid for promoting animal growth is characterized in that the main drug is macleaya cordata extract, wherein the main active ingredients are macleaya cordata total alkaloids and macleaya cordata leaf and stem volatile oil, the content of the total alkaloids in each milliliter of macleaya cordata extract of a semi-finished product is more than or equal to 10mg, and the content of the volatile oil is equal to more than or equal to 1g of the original drug.
8. A preparation method of an oral liquid for promoting animal growth is characterized by comprising the following steps: mixing the extract of stem and leaf of herba Macleayae Cordatae with the extract of fruit of herba Macleayae Cordatae to obtain herba Macleayae Cordatae extract, diluting with distilled water to obtain diluent, adjusting pH to 4-6, adding cosolvent, correctant and antiseptic, stirring, filtering, quality testing, quantitatively bottling, sealing, and packaging.
9. The method for preparing oral liquid for promoting animal growth according to claim 8, wherein the content of total alkaloids in each milliliter of the diluted solution is not less than 5 mg.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110425623.3A CN113318150A (en) | 2021-04-20 | 2021-04-20 | Application of macleaya cordata extract in preparation of oral liquid for promoting animal growth |
| ZA2022/03896A ZA202203896B (en) | 2021-04-20 | 2022-04-06 | Application of macleaya cordata extract in preparation of oral liquid for promoting animal growth |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110425623.3A CN113318150A (en) | 2021-04-20 | 2021-04-20 | Application of macleaya cordata extract in preparation of oral liquid for promoting animal growth |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113318150A true CN113318150A (en) | 2021-08-31 |
Family
ID=77414934
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110425623.3A Pending CN113318150A (en) | 2021-04-20 | 2021-04-20 | Application of macleaya cordata extract in preparation of oral liquid for promoting animal growth |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN113318150A (en) |
| ZA (1) | ZA202203896B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113425676A (en) * | 2021-07-07 | 2021-09-24 | 河北农业大学 | Oregano macleaya cordata oral solution and preparation method thereof |
| CN114766594A (en) * | 2022-04-12 | 2022-07-22 | 圣道生物技术(山东)有限公司 | Plant extract bacterium enzyme biological preparation for resisting inflammation and promoting growth of livestock and poultry and preparation method thereof |
| CN115054942A (en) * | 2022-06-09 | 2022-09-16 | 珠海科技学院 | Preparation method and application of macleaya cordata extract |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530475A (en) * | 2009-04-17 | 2009-09-16 | 湖南美可达生物资源有限公司 | Application of pink plumepoppy herb extract in veterinary drugs of economic animals |
| CN104137949A (en) * | 2013-07-19 | 2014-11-12 | 安佑生物科技集团有限公司 | Method for application of macleaya cordata plant in feed |
| CN108837006A (en) * | 2018-07-24 | 2018-11-20 | 湖南美可达生物资源股份有限公司 | A kind of macleaya cordata leaf extract and preparation method thereof and product |
-
2021
- 2021-04-20 CN CN202110425623.3A patent/CN113318150A/en active Pending
-
2022
- 2022-04-06 ZA ZA2022/03896A patent/ZA202203896B/en unknown
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530475A (en) * | 2009-04-17 | 2009-09-16 | 湖南美可达生物资源有限公司 | Application of pink plumepoppy herb extract in veterinary drugs of economic animals |
| CN104137949A (en) * | 2013-07-19 | 2014-11-12 | 安佑生物科技集团有限公司 | Method for application of macleaya cordata plant in feed |
| CN108837006A (en) * | 2018-07-24 | 2018-11-20 | 湖南美可达生物资源股份有限公司 | A kind of macleaya cordata leaf extract and preparation method thereof and product |
Non-Patent Citations (1)
| Title |
|---|
| 李春梅等: "博落回根茎叶挥发油化学成分及抗菌活性研究", 《化学与生物工程》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113425676A (en) * | 2021-07-07 | 2021-09-24 | 河北农业大学 | Oregano macleaya cordata oral solution and preparation method thereof |
| CN114766594A (en) * | 2022-04-12 | 2022-07-22 | 圣道生物技术(山东)有限公司 | Plant extract bacterium enzyme biological preparation for resisting inflammation and promoting growth of livestock and poultry and preparation method thereof |
| CN115054942A (en) * | 2022-06-09 | 2022-09-16 | 珠海科技学院 | Preparation method and application of macleaya cordata extract |
Also Published As
| Publication number | Publication date |
|---|---|
| ZA202203896B (en) | 2022-06-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113318150A (en) | Application of macleaya cordata extract in preparation of oral liquid for promoting animal growth | |
| KR101061257B1 (en) | Natural antibiotics using bee venom and herbal extracts as an active ingredient and preparation method thereof | |
| CN102630816A (en) | Camphor tree branch and leave extracting feed additive and preparation method thereof | |
| KR100920713B1 (en) | A composition for the treatment or prevention of diarrhea | |
| CN109645232A (en) | A kind of Chinese herbal medicine composite feed additive for treating animal diarrhea | |
| CN109316597B (en) | Traditional Chinese medicine preparation with antibacterial and liver-protecting functions for freshwater fish and preparation method thereof | |
| CN113332340A (en) | Plant compound extract and application thereof in preparation of macleaya cordata oral liquid | |
| CN100335073C (en) | Process for preparing semen momordicae seed extract containing triterpene saponin component | |
| CN104825700A (en) | Traditional Chinese medicine composition for resisting bacteria and diminishing inflammation | |
| CN111358850B (en) | Traditional Chinese medicine composition for preventing and treating chicken colibacillosis and preparation method thereof | |
| CN104758516B (en) | Eupatorium adenophorum composition for treating chicken coccidiosis and preparation method thereof | |
| CN111990533A (en) | Fermented eucommia ulmoides leaf extract and application thereof in preparation of chicken feed | |
| EP2793917B1 (en) | Extract of rhus copallina extract for use as a medicament | |
| CN111281922B (en) | Compound traditional Chinese veterinary medicine oral preparation for treating diarrhea of pigs and poultry and preparation method thereof | |
| CN113599432B (en) | Traditional Chinese medicine for preventing bacterial enteritis of grass carp and preparation method thereof | |
| CN114984117A (en) | Vine tea flavone extract and application thereof in preparation of compound vine tea flavone oral liquid | |
| KR20130013965A (en) | Anti-fungal and anti-bacterial compositions against fish pathogenic bacteria | |
| CN110974878B (en) | Traditional Chinese medicine spray for treating avian infectious rhinotracheitis of green-shell laying hens and preparation method thereof | |
| CN115177679B (en) | Preparation technology of veterinary isatis root granules for improving content and palatability of active ingredients | |
| CN115137020A (en) | Anti-inflammatory feed composition for ruminants and preparation method thereof | |
| CN117298186A (en) | Compound medicament for treating trichomonas pigeon, preparation method and medicament thereof | |
| CN1927251B (en) | Medicament for animals with heat clearing and detoxicating functions and preparing method thereof | |
| CN105079175A (en) | Application of garden burnet or extract thereof in preparing veterinary drug for treating animal diarrhea | |
| CN114306554A (en) | A compound Chinese medicinal preparation for broad-spectrum antibacterial of animals, and its preparation method | |
| CN113768990A (en) | Traditional Chinese medicine composition for preventing and treating infectious bursal disease of chicken |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210831 |