CN115177679B - Preparation technology of veterinary isatis root granules for improving content and palatability of active ingredients - Google Patents
Preparation technology of veterinary isatis root granules for improving content and palatability of active ingredients Download PDFInfo
- Publication number
- CN115177679B CN115177679B CN202210779299.XA CN202210779299A CN115177679B CN 115177679 B CN115177679 B CN 115177679B CN 202210779299 A CN202210779299 A CN 202210779299A CN 115177679 B CN115177679 B CN 115177679B
- Authority
- CN
- China
- Prior art keywords
- percolate
- leaching
- concentrating
- mixing
- decoction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000334160 Isatis Species 0.000 title claims abstract description 50
- 239000008187 granular material Substances 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 239000004480 active ingredient Substances 0.000 title abstract description 15
- 235000019629 palatability Nutrition 0.000 title abstract description 11
- 238000005516 engineering process Methods 0.000 title description 4
- 238000002386 leaching Methods 0.000 claims abstract description 84
- 239000000284 extract Substances 0.000 claims abstract description 31
- 239000007853 buffer solution Substances 0.000 claims abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 61
- 238000002156 mixing Methods 0.000 claims description 56
- 239000000243 solution Substances 0.000 claims description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 239000010231 banlangen Substances 0.000 claims description 27
- 241000334154 Isatis tinctoria Species 0.000 claims description 26
- 239000000706 filtrate Substances 0.000 claims description 26
- 238000002791 soaking Methods 0.000 claims description 25
- -1 alkyl glucoside Chemical class 0.000 claims description 18
- 239000002245 particle Substances 0.000 claims description 18
- 229930182478 glucoside Natural products 0.000 claims description 17
- 239000000463 material Substances 0.000 claims description 17
- 229930006000 Sucrose Natural products 0.000 claims description 15
- 239000002244 precipitate Substances 0.000 claims description 15
- 239000005720 sucrose Substances 0.000 claims description 15
- 238000005119 centrifugation Methods 0.000 claims description 14
- 238000001816 cooling Methods 0.000 claims description 14
- 229920000858 Cyclodextrin Polymers 0.000 claims description 13
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 13
- 238000010298 pulverizing process Methods 0.000 claims description 13
- 239000006228 supernatant Substances 0.000 claims description 13
- 239000001116 FEMA 4028 Substances 0.000 claims description 12
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical group OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 12
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims description 12
- 229960004853 betadex Drugs 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 12
- 238000005520 cutting process Methods 0.000 claims description 10
- 239000008055 phosphate buffer solution Substances 0.000 claims description 10
- 239000000796 flavoring agent Substances 0.000 claims description 5
- 235000013355 food flavoring agent Nutrition 0.000 claims description 5
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 5
- 125000000185 sucrose group Chemical group 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 5
- 238000000034 method Methods 0.000 abstract description 25
- 239000003814 drug Substances 0.000 abstract description 17
- 238000005325 percolation Methods 0.000 abstract description 14
- 229940079593 drug Drugs 0.000 abstract description 5
- 239000004615 ingredient Substances 0.000 abstract description 5
- 235000019658 bitter taste Nutrition 0.000 abstract description 4
- 244000144977 poultry Species 0.000 abstract description 3
- 229940126680 traditional chinese medicines Drugs 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 16
- 238000012360 testing method Methods 0.000 description 13
- 238000001035 drying Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 230000002401 inhibitory effect Effects 0.000 description 9
- 241000287828 Gallus gallus Species 0.000 description 8
- VQQVWGVXDIPORV-UHFFFAOYSA-N Tryptanthrine Chemical compound C1=CC=C2C(=O)N3C4=CC=CC=C4C(=O)C3=NC2=C1 VQQVWGVXDIPORV-UHFFFAOYSA-N 0.000 description 8
- 230000000844 anti-bacterial effect Effects 0.000 description 7
- 235000013330 chicken meat Nutrition 0.000 description 7
- 241000193996 Streptococcus pyogenes Species 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 4
- 241000282898 Sus scrofa Species 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 238000005469 granulation Methods 0.000 description 4
- 230000003179 granulation Effects 0.000 description 4
- JXDYKVIHCLTXOP-UHFFFAOYSA-N isatin Chemical compound C1=CC=C2C(=O)C(=O)NC2=C1 JXDYKVIHCLTXOP-UHFFFAOYSA-N 0.000 description 4
- 238000012795 verification Methods 0.000 description 4
- 241000606748 Actinobacillus pleuropneumoniae Species 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 241000191967 Staphylococcus aureus Species 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 150000002475 indoles Chemical class 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229920001661 Chitosan Polymers 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 229930013930 alkaloid Natural products 0.000 description 2
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- 238000005352 clarification Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000021050 feed intake Nutrition 0.000 description 2
- 238000005189 flocculation Methods 0.000 description 2
- 230000016615 flocculation Effects 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 235000013594 poultry meat Nutrition 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 208000011580 syndromic disease Diseases 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- 238000009631 Broth culture Methods 0.000 description 1
- 208000004232 Enteritis Diseases 0.000 description 1
- 201000000297 Erysipelas Diseases 0.000 description 1
- 206010069767 H1N1 influenza Diseases 0.000 description 1
- 206010020843 Hyperthermia Diseases 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- 229920000715 Mucilage Polymers 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 1
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000009620 Orthomyxoviridae Infections Diseases 0.000 description 1
- 206010033971 Paratyphoid fever Diseases 0.000 description 1
- 241000125945 Protoparvovirus Species 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 150000004056 anthraquinones Chemical class 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 229940054051 antipsychotic indole derivative Drugs 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 238000002481 ethanol extraction Methods 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 230000036031 hyperthermia Effects 0.000 description 1
- 230000008629 immune suppression Effects 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000004634 pharmacological analysis method Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 229940126532 prescription medicine Drugs 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 208000009305 pseudorabies Diseases 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical group O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 208000013223 septicemia Diseases 0.000 description 1
- 235000019832 sodium triphosphate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 201000010740 swine influenza Diseases 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 238000005550 wet granulation Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
- A61K36/855—Clerodendrum, e.g. glorybower
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
- A61P31/22—Antivirals for DNA viruses for herpes viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Epidemiology (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Rheumatology (AREA)
- Pulmonology (AREA)
- Medicines Containing Plant Substances (AREA)
- Pain & Pain Management (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a preparation process of veterinary isatis root granules for improving the content of active ingredients and palatability. The preparation process of the isatis root granules mainly optimizes and decomposes the leaching process, extracts active ingredients and auxiliary ingredients to the greatest extent through double leaching by a decoction method and a percolation method, avoids the leaching of invalid ingredients and tissue ingredients, and simultaneously maintains the pH stability of the decoction by adding a proper amount of buffer solution in the leaching process of the decoction method, reduces the leaching of the invalid ingredients, reduces bitter taste and improves the medication compliance of poultry.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a preparation process of isatis root granules for improving active ingredients.
Background
The radix Isatidis granule is a traditional antiviral Chinese medicine. The clinical medicine generally can be used for treating and preventing the immune suppression diseases such as swine influenza, hyperthermia, breeding and breathing syndrome, parvovirus, exudative septicemia, erysipelas, streptococcus infection, paratyphoid fever, viral enteritis, eperythrozoon, pseudorabies, postweaning multisystem syndrome and the like by using the isatis root granules as one of prescription medicines.
As is well known, the isatis root granules are granules prepared from isatis root and dyers woad leaf extracts, the isatis root and dyers woad leaf are both derived from cruciferae plant Isatis indigotica, the root of which is isatis root and the leaf of which is dyer woad leaf.
According to pharmacological analysis, the isatis root and the dyers woad leaf have broad-spectrum antibacterial effects, the active ingredients of the antibacterial effects are tryptanthrin and indole derivatives, the dyers woad leaf with high concentration has bactericidal effects, and the dyers woad leaf has antiviral effects. Modern pharmacological studies prove that the radix isatidis particles have wide functions of resisting virus, bacteria, tumors, endotoxin, immunity and the like.
In the prior art, the preparation process of the isatis root and dyers woad leaf granules comprises the steps of mixing isatis root and dyers woad leaf, decocting, concentrating filtrate, mixing thick paste obtained by concentrating filtrate with auxiliary materials, granulating, and the like, wherein the thick paste is mainly prepared by adopting a traditional hydroalcoholic extraction method, wet granulation and the like, and impurities such as protein, starch, mucilage, grease, fat-soluble pigment, resin, gum, part of sugar and the like in an extracting solution are removed to a certain extent, but the preparation process still has a plurality of defects such as high loss of active ingredients, low content, direct caused efficacy weakness, slow effect, delayed illness state and low cure rate.
Chinese patent CN103006914 adopts flocculation clarification technology to prepare green granules, and adopts ZTC1+1-II type clarifier adsorption mode to remove coarse granules in solution so as to attain the goal of refining medicament. The calculation shows that the loss rate of the effective components of the traditional Chinese medicine of the medicament prepared by the method is 12-17%, and the loss rate of the effective components of the traditional Chinese medicine in the medicament prepared by the water extraction and alcohol precipitation method in the prior art is about 37%, so that the loss rate of the effective components of the traditional Chinese medicine is reduced. However, flocculation clarification technology has higher difficulty, ZTC1+1-II type clarifier has higher cost, and industrial production is difficult to realize.
The Chinese patent CN107898876 provides a green particle process, which is added with betaine, chitosan with small molecular weight and sodium tripolyphosphate in the process of decoction and extraction, and compared with the conventional process recorded in Chinese veterinary pharmacopoeia, the antibacterial activity of pathogenic bacteria of livestock and poultry is obviously improved. The polysaccharide substances such as chitosan and the like have the immunoregulation function, and under the action of the main medicine, the polysaccharide substances can assist in improving the immunity of organisms, thereby improving the curative effect of the medicine.
Disclosure of Invention
The invention provides a preparation process of veterinary radix isatidis granules capable of obviously improving active ingredients.
Specifically, the technical scheme of the invention is as follows:
a preparation process of radix Isatidis granule comprises four steps of pretreatment of medicinal materials, leaching, separation and concentration, and granulation.
The pretreatment of the medicinal materials in the step (1) is to cut up the isatis root and the dyers woad leaf for standby.
The step (2) of leaching is to leach the effective components of the pretreated isatis root and the isatis root She Tong by water decoction leaching and percolation leaching, and the decoction filtrate and the percolation liquid are mixed to obtain leaching liquid.
And (3) separating and concentrating, removing precipitate in the leaching liquor by centrifugation, and concentrating to obtain an extract.
And (4) granulating, namely cooling the extract, adding an excipient and a flavoring agent, and granulating.
Further, the water decoction leaching step in the step (2) is as follows: mixing the pretreated radix Isatidis and folium Isatidis, adding 5-8 times of water, adding buffer solution to adjust pH to 7.2-8.0, decocting for 2 times each for 1 hr, mixing decoctions, and filtering to obtain decoction filtrate.
The aim is to leach out the water-soluble effective components in the medicinal materials, wherein the specific effective components include, but are not limited to, amino acids, anthraquinone compounds, tryptanthrin, alkaloids, sugar and the like. In addition, the purpose of adding the buffer solution is that the pH value of the decoction is continuously changed in the process of decocting medicinal materials, the addition of the buffer solution can keep the decoction within a specific pH range, the inventor finds that the decoction of the isatis root and the dyers woad leaf is slightly alkaline, the decoction becomes thick along with the increase of the decoction time in the process of decoction, and the alkalinity is gradually increased, which is one of the important reasons of bitter taste, and therefore the inventor tries to add the buffer solution into the decoction, so that the pH value of the decoction is kept within a range of 7.2-8.0 slightly alkaline, and the bitter taste is reduced. Preferably, the buffer solution is phosphate buffer, ph=7.5-7.8.
Further, the percolation leaching in the step (2) comprises primary percolation and secondary percolation.
Wherein, the primary percolation step is as follows: collecting the residue after decoction, pulverizing the residue again, placing in a percolator, adding 50% ethanol, adding alkyl glucoside, soaking for 12-18 hr, percolating, collecting 3/4 primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to obtain percolate I.
Wherein, the secondary percolation step is: adding 70% ethanol into a percolator, soaking for 8-15 hr, percolating, collecting 3/4 of primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to obtain percolate I.
The purpose of the two percolating is to extract and leach the effective components to the maximum extent by using ethanol with different volume fractions. The first percolation is performed by ethanol extraction with volume fraction of 50%, the extracted components include but are not limited to residual alkaloids, tryptanthrin and alcohols, the second percolation is performed by ethanol with volume fraction of 70% with weaker polarity, and the extracted components include but are not limited to isatin, organic acid, lipid, anthraquinone and indole compounds. In addition, the inventor adds a small amount of alkyl glucoside in the first percolation process, so that the leaching effect of ethanol and the stability of active ingredients are improved, the decomposition of alcohols and glycoside substances is avoided, and especially, the dosage of the alkyl glucoside is 1/15-1/50 of the weight of isatis root and dyers woad leaf, the adding amount of the alkyl glucoside is too small, the effect of improving the leaching effect and the stability of the active ingredients is not obvious, and the adding amount can influence the interaction of the active ingredients and auxiliary ingredients.
And (3) removing solid precipitate in the leaching solution in the step (2) by centrifugation, taking supernatant, and concentrating to obtain extract with the density of 1.1-1.3 at normal pressure for later use.
And (4) cooling the extract, adding an excipient and a flavoring agent, and granulating. The excipient is cyclodextrin, the flavoring agent is selected from sucrose, but not limited to sucrose, and the main purpose of the sucrose is to reduce cost of veterinary medicines.
In summary, the preparation process of the isatis root granules of the invention specifically comprises the following steps:
(1) Pretreatment of medicinal materials: chopping radix Isatidis and folium Isatidis for use;
(2) Leaching:
decocting and leaching with water: mixing the pretreated radix Isatidis and folium Isatidis, adding 5-8 times of water, adding phosphate buffer solution to adjust pH to 7.5-7.8, decocting for 2 times each for 1 hr, mixing decoctions, and filtering to obtain decoction filtrate;
percolating and leaching: collecting the decoction dregs, pulverizing the decoction dregs again, placing the decoction dregs into a percolator, adding 50% ethanol, adding alkyl glucoside in an amount which is 1/15-1/50 of the weight of the isatis root and the dyers woad leaf, soaking for 12-18h, percolating, collecting 3/4 primary percolate, concentrating the secondary percolate at a low temperature, and combining the secondary percolate with the primary percolate to form a percolate I; adding 70% ethanol into a percolator, soaking for 8-15h, percolating, collecting 3/4 of primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to form percolate I;
mixing the decoction filtrate, the percolate I and the percolate I to obtain a leaching solution;
(3) Separating and concentrating: removing precipitate in the leaching solution in the step (2) by centrifugation, taking supernatant, and concentrating to obtain extract with density of 1.1-1.3 at normal pressure;
(4) Granulating: and cooling the extract, adding the crushed beta-cyclodextrin and sucrose, and granulating.
Compared with the prior art, the invention has the beneficial effects that:
(1) In the preparation process of the isatis root granules, the leaching process is mainly optimized, and the effective components and the auxiliary components are leached to the maximum extent through double leaching by a decoction method and a percolation method, so that the leaching of ineffective components and tissue components is avoided.
(2) Proper amount of buffer solution is added in the leaching process of the decoction method to keep the pH of the decoction stable, reduce the leaching of invalid components, reduce bitter taste and improve the medication compliance of poultry.
(3) The effective components are leached respectively by two times of ethanol with different volume fractions in the percolation leaching, and a certain amount of alkyl glucoside is added in the first percolation process, so that the leaching effect of the ethanol and the stability of the effective components are improved, the content of the effective components in the percolate is high, and the content of the effective components in the further prepared isatis root granules is high, and the medicine effect is good.
Drawings
FIG. 1 minimum inhibitory concentration of Isatis-root granulation on Streptococcus pyogenes of examples 1-4, comparative examples 1-6
FIG. 2 minimum inhibitory concentration of Isatis-associated granulation on Staphylococcus aureus for examples 1-4, comparative examples 1-6
FIG. 3 minimum inhibitory concentration of Isoque granulating of examples 1-4, comparative examples 1-6 on Actinobacillus pleuropneumoniae
FIG. 4 minimum inhibitory concentration of Isatis-root granulation of examples 1-4, comparative examples 1-6 on Streptococcus hemolyticus
FIG. 5 minimum inhibitory concentration of Isoque particles of examples 1 to 4 and comparative examples 1 to 6 on E.coli
Detailed Description
The present invention will be further described with reference to examples for the purpose of making the objects and technical aspects of the present invention more apparent, but the scope of the present invention is not limited to these examples, which are only for explaining the present invention. It will be understood by those skilled in the art that variations or equivalent substitutions that do not depart from the spirit of the invention are intended to be included within the scope of the invention.
Example 1 preparation Process of veterinary Isatis Equipped particles
(1) Pretreatment of medicinal materials: 600g of radix isatidis and 900g of dyers woad leaf, and cutting up for later use;
(2) Leaching:
decocting and leaching with water: mixing the pretreated radix Isatidis and folium Isatidis, adding 6 times of water, adding phosphate buffer solution to adjust pH to 7.8, decocting for 2 times each for 1 hr, mixing decoctions, and filtering to obtain decoction filtrate;
percolating and leaching: collecting the decoction dregs, pulverizing the decoction dregs again, placing the decoction dregs in a percolator, adding 50% ethanol, adding 50g of alkyl glucoside, soaking for 15 hours, percolating, collecting 3/4 primary percolate, concentrating the secondary percolate at low temperature, and combining with the primary percolate to form a percolate I; adding 70% ethanol into a percolator, soaking for 10h, percolating, collecting 3/4 of primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to form a percolate I;
mixing the decoction filtrate, the percolate I and the percolate I to obtain a leaching solution;
(3) Separating and concentrating: removing precipitate in the leaching solution in the step (2) by centrifugation, taking supernatant, and concentrating to obtain extract with density of 1.2 at normal pressure;
(4) Granulating: and cooling the extract, adding the crushed beta-cyclodextrin and sucrose, granulating, drying and preparing 1500g.
Example 2 preparation Process of veterinary Isatis Equipped particles
(1) Pretreatment of medicinal materials: 600g of radix isatidis and 900g of dyers woad leaf, and cutting up for later use;
(2) Leaching:
decocting and leaching with water: mixing the pretreated radix Isatidis and folium Isatidis, adding 5 times of water, adding phosphate buffer solution to adjust pH to 7.5, decocting for 2 times each for 1 hr, mixing decoctions, and filtering to obtain decoction filtrate;
percolating and leaching: collecting the decoction dregs, pulverizing the decoction dregs again, placing the decoction dregs in a percolator, adding 50% ethanol, adding 30g of alkyl glucoside, soaking for 12 hours, percolating, collecting 3/4 primary percolate, concentrating the secondary percolate at low temperature, and combining with the primary percolate to form a percolate I; adding 70% ethanol into a percolator, soaking for 8h, percolating, collecting 3/4 of primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to form a percolate I;
mixing the decoction filtrate, the percolate I and the percolate I to obtain a leaching solution;
(3) Separating and concentrating: removing precipitate in the leaching solution in the step (2) by centrifugation, taking supernatant, and concentrating to obtain extract with density of 1.1 at normal pressure;
(4) Granulating: and cooling the extract, adding the crushed beta-cyclodextrin and sucrose, granulating, drying and preparing 1500g.
Example 3 preparation Process of veterinary Isatis Equipped particles
(1) Pretreatment of medicinal materials: 600g of radix isatidis and 900g of dyers woad leaf, and cutting up for later use;
(2) Leaching:
decocting and leaching with water: mixing the pretreated radix Isatidis and folium Isatidis, adding water 8 times of its total weight, adding phosphate buffer solution to adjust pH to 8.0, decocting for 2 times each for 1 hr, mixing decoctions, and filtering to obtain decoction filtrate;
percolating and leaching: collecting the decocted medicinal residues, pulverizing the medicinal residues again, placing in a percolator, adding 50% ethanol, adding 80g of alkyl glucoside, soaking for 18 hr, percolating, collecting 3/4 primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to form percolate I; adding 70% ethanol into a percolator, soaking for 15h, percolating, collecting 3/4 of primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to form a percolate I;
mixing the decoction filtrate, the percolate I and the percolate I to obtain a leaching solution;
(3) Separating and concentrating: removing precipitate in the leaching solution in the step (2) by centrifugation, taking supernatant, and concentrating to obtain extract with density of 1.3 at normal pressure;
(4) Granulating: and cooling the extract, adding the crushed beta-cyclodextrin and sucrose, granulating, drying and preparing 1500g.
Example 4 preparation Process of veterinary Isatis Equipped particles
(1) Pretreatment of medicinal materials: 600g of radix isatidis and 900g of dyers woad leaf, and cutting up for later use;
(2) Leaching:
decocting and leaching with water: mixing the pretreated radix Isatidis and folium Isatidis, adding 6 times of water, adding phosphate buffer solution to adjust pH to 7.2, decocting for 2 times each for 1 hr, mixing decoctions, and filtering to obtain decoction filtrate;
percolating and leaching: collecting the decocted medicinal residues, pulverizing the medicinal residues again, placing in a percolator, adding 50% ethanol, adding 100g of alkyl glucoside, soaking for 14h, percolating, collecting 3/4 primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to form percolate I; adding 70% ethanol into a percolator, soaking for 12h, percolating, collecting 3/4 of primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to form a percolate I;
mixing the decoction filtrate, the percolate I and the percolate I to obtain a leaching solution;
(3) Separating and concentrating: removing precipitate in the leaching solution in the step (2) by centrifugation, taking supernatant, and concentrating to obtain extract with density of 1.15 at normal pressure;
(4) Granulating: and cooling the extract, adding the crushed beta-cyclodextrin and sucrose, granulating, drying and preparing 1500g. Comparative example 1 preparation process of veterinary Isatis indigotica fort particles
(1) Pretreatment of medicinal materials: 600g of radix isatidis and 900g of dyers woad leaf, and cutting up for later use;
(2) Leaching:
decocting and leaching with water: mixing the pretreated radix Isatidis and folium Isatidis, adding 6 times of water, decocting for 2 times each for 1 hr, mixing decoctions, and filtering to obtain decoction filtrate;
percolating and leaching: collecting the decoction dregs, pulverizing the decoction dregs again, placing the decoction dregs in a percolator, adding 50% ethanol, adding 50g of alkyl glucoside, soaking for 15 hours, percolating, collecting 3/4 primary percolate, concentrating the secondary percolate at low temperature, and combining with the primary percolate to form a percolate I; adding 70% ethanol into a percolator, soaking for 10h, percolating, collecting 3/4 of primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to form a percolate I;
mixing the decoction filtrate, the percolate I and the percolate I to obtain a leaching solution;
(3) Separating and concentrating: removing precipitate in the leaching solution in the step (2) by centrifugation, taking supernatant, and concentrating to obtain extract with density of 1.2 at normal pressure;
(4) Granulating: and cooling the extract, adding the crushed beta-cyclodextrin and sucrose, granulating, drying and preparing 1500g. Comparative example 2 preparation process of veterinary isatis root granules
(1) Pretreatment of medicinal materials: 600g of radix isatidis and 900g of dyers woad leaf, and cutting up for later use;
(2) Leaching:
decocting and leaching with water: mixing the pretreated radix Isatidis and folium Isatidis, adding 6 times of water, adding phosphate buffer solution to adjust pH to 6.8, decocting for 2 times each for 1 hr, mixing decoctions, and filtering to obtain decoction filtrate;
percolating and leaching: collecting the decoction dregs, pulverizing the decoction dregs again, placing the decoction dregs in a percolator, adding 50% ethanol, adding 50g of alkyl glucoside, soaking for 15 hours, percolating, collecting 3/4 primary percolate, concentrating the secondary percolate at low temperature, and combining with the primary percolate to form a percolate I; adding 70% ethanol into a percolator, soaking for 10h, percolating, collecting 3/4 of primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to form a percolate I;
mixing the decoction filtrate, the percolate I and the percolate I to obtain a leaching solution;
(3) Separating and concentrating: removing precipitate in the leaching solution in the step (2) by centrifugation, taking supernatant, and concentrating to obtain extract with density of 1.2 at normal pressure;
(4) Granulating: and cooling the extract, adding the crushed beta-cyclodextrin and sucrose, granulating, drying and preparing 1500g. Comparative example 3 preparation process of veterinary Isatis indigotica fort particles
(1) Pretreatment of medicinal materials: 600g of radix isatidis and 900g of dyers woad leaf, and cutting up for later use;
(2) Leaching:
percolating and leaching: collecting the decoction dregs, pulverizing the decoction dregs again, placing the decoction dregs in a percolator, adding 50% ethanol, adding 50g of alkyl glucoside, soaking for 15 hours, percolating, collecting 3/4 primary percolate, concentrating the secondary percolate at low temperature, and combining with the primary percolate to form a percolate I; adding 70% ethanol into a percolator, soaking for 10h, percolating, collecting 3/4 of primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to form a percolate I;
mixing the percolate I and the percolate I to obtain a leaching solution;
(3) Separating and concentrating: removing precipitate in the leaching solution in the step (2) by centrifugation, taking supernatant, and concentrating to obtain extract with density of 1.2 at normal pressure;
(4) Granulating: and cooling the extract, adding the crushed beta-cyclodextrin and sucrose, granulating, drying and preparing 1500g. Comparative example 4 preparation process of veterinary isatis root granules
(1) Pretreatment of medicinal materials: 600g of radix isatidis and 900g of dyers woad leaf, and cutting up for later use;
(2) Leaching:
decocting and leaching with water: mixing the pretreated radix Isatidis and folium Isatidis, adding 6 times of water, adding phosphate buffer solution to adjust pH to 7.8, decocting for 2 times each for 1 hr, mixing decoctions, and filtering to obtain decoction filtrate;
percolating and leaching: collecting the decoction dregs, pulverizing the decoction dregs again, placing the decoction dregs in a percolator, adding 50% ethanol, adding 50g of alkyl glucoside, soaking for 15 hours, percolating, collecting 3/4 primary percolate, concentrating the secondary percolate at low temperature, and combining with the primary percolate to form a percolate;
mixing the decoction filtrate and the percolate to obtain a leaching solution;
(3) Separating and concentrating: removing precipitate in the leaching solution in the step (2) by centrifugation, taking supernatant, and concentrating to obtain extract with density of 1.2 at normal pressure;
(4) Granulating: and cooling the extract, adding the crushed beta-cyclodextrin and sucrose, granulating, drying and preparing 1500g. Comparative example 5 preparation process of veterinary radix Isatidis granule
(1) Pretreatment of medicinal materials: 600g of radix isatidis and 900g of dyers woad leaf, and cutting up for later use;
(2) Leaching:
decocting and leaching with water: mixing the pretreated radix Isatidis and folium Isatidis, adding 6 times of water, adding phosphate buffer solution to adjust pH to 7.8, decocting for 2 times each for 1 hr, mixing decoctions, and filtering to obtain decoction filtrate;
percolating and leaching: collecting the decoction dregs, pulverizing the decoction dregs again, placing the decoction dregs in a percolator, adding 50% ethanol, soaking for 15h, percolating, collecting 3/4 of primary percolate, concentrating the secondary percolate at low temperature, and combining with the primary percolate to form a percolate I; adding 70% ethanol into a percolator, soaking for 10h, percolating, collecting 3/4 of primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to form a percolate I;
mixing the decoction filtrate, the percolate I and the percolate I to obtain a leaching solution;
(3) Separating and concentrating: removing precipitate in the leaching solution in the step (2) by centrifugation, taking supernatant, and concentrating to obtain extract with density of 1.2 at normal pressure;
(4) Granulating: and cooling the extract, adding the crushed beta-cyclodextrin and sucrose, granulating, drying and preparing 1500g. Comparative example 6:
the preparation method of the isatis root granules is the same as the standard of the 2015 edition of the animal pharmacopoeia of the people's republic of China.
Verification embodiment
1. Verification of antibacterial effect
To investigate the antimicrobial activity of the plate green particles of each example and comparative example, the bacteriostatic effect was measured using the Minimum Inhibitory Concentration (MIC), which is the minimum inhibitory concentration at which bacterial growth was completely inhibited in the tube or well in a dilution method for microbiological identification. The method refers to the test method of the bacteriostasis effect of the paragraphs [0037] to [0042] of the specification of Chinese patent CN107898876, and is obtained after adjustment according to the specific situation of the invention.
The method comprises the following specific operations:
(1) Culturing bacterial liquid: inoculating Streptococcus pyogenes, staphylococcus aureus, actinobacillus pleuropneumoniae, streptococcus hemolyticus, and Escherichia coli into MH broth culture medium, inoculating the above 5 activated strains into slant culture medium, culturing in 37 deg.C incubator for 24 hr, diluting the strain in slant culture medium with nutrient broth to obtain bacterial solutions with concentration of 10 6 CFU/ml, ready for use.
(2) Sample treatment: 5g of each of the plate green granules prepared in examples 1 to 4 and comparative examples 1 to 6 was placed in a 150m1 triangular flask, and a sample solution containing 0.5g/ml of the plate green granules was prepared with distilled water, autoclaved and stored in a refrigerator at 4℃for use.
(3) Determination of MIC: taking 11 sterile test tubes, adding 1ml of sterile distilled water, respectively taking 1.0ml of sample solutions of examples 1-4 and comparative examples 1-6, respectively transferring into test tubes 1-10, uniformly mixing, and performing gradient dilution, wherein the test tubes 11 are drug-free controls. The minimum concentration of drug contained without bacterial growth was the Minimum Inhibitory Concentration (MIC) observed in culture with 50% mh broth.
The above test is repeated 3 times in parallel to ensure the accuracy and precision of the test result.
The antibacterial effect is shown in figures 1-5, and the minimum concentration of the veterinary isatis root particles for inhibiting streptococcus pyogenes, staphylococcus aureus, actinobacillus pleuropneumoniae, streptococcus hemolyticus and escherichia coli is smaller in the example 1-4, so that the antibacterial effect of the veterinary isatis root particles is better, and the fact that the content of active ingredients in the veterinary isatis root particles is higher is suggested.
2. Verification of the content of active ingredients
In order to verify whether the process of the invention can improve the content of the active ingredients in the isatis leaf particles, the isatis leaf particles prepared in examples 1-4 and comparative examples 1-6 are respectively subjected to content measurement of arginine, isatin, tryptanthrin and indole compounds. The measuring method is finished by referring to detection methods of the pharmacopoeia of the people's republic of China, the Chinese pharmacopoeia, the Chinese patent CN103575818 and the like.
TABLE 1 content of active ingredient in examples 1-4, comparative examples 1-6 ted green pellets
As can be seen from Table 1, the effective component content of the isatis root granules prepared by the preparation process is high, which indicates that the optimized preparation process can obviously improve the effective component content and further improve the curative effect.
3. Palatability verification of green-board particles
On the premise of safety research, the palatability of the isatis root granules and the commercial isatis root granules is compared by a double-basin method. Two basins of the same food are added with the isatis root granules of the invention, and are uniformly stirred, and the other basin is added with the commercially available isatis root granules, and the two basins are simultaneously placed in a feeding area to feed animals, and the feeding amount in the two basins is calculated respectively, so that the palatability of the animals to the medicine is measured.
The palatability test method specifically involves the following steps:
sample: 40 healthy pigs in a region (pig species: white pigs) of the Linshu county in Shandong province and 40 healthy chickens in a region (chicken species: spotted-brown chickens) of the Linshu county in Shandong province and 30 days old are selected.
Dosage is as follows: the dosage of the isatis root granules and the commercial isatis root granules is 0.2g per kilogram of body weight.
Pig palatability test design: fasted after 6 days and late before the test, fasted for 12 hours, fed for the first time at 6 days and fed for the same initial weight of food in the two basins, calculated dose of the example 1 green pellets are put in the basin A, the same dose of the commercial green pellets are put in the basin B, fasted after 6 days and fasted after 12 hours, fed for 6 days and fed for the same content (fed twice a day) at 6 days and the test is repeated for 5 days, wherein the basins need to be exchanged for the left and right in the morning and evening in the test stage, and the preference error of the animal to the direction is reduced. After the test was completed, the feed rates (IR) of the a-basin and B-basin foods were calculated, respectively, referring to the sum of the feed intake of one test feed divided by the feed intake of the two control samples, IR (a) =a/(a+b).
Chicken palatability test design: the food amount and the dose are calculated according to the chicken dose and the food amount by the method.
Table 2 feeding rate of basin a and basin B foods for chicken and pig samples
| Feeding rate of basin A (%) | B basin feeding rate (%) | |
| Chicken (chicken) | 60.41±3.88 | 39.59±2.74 |
| Pig | 65.56±4.01 | 34.44±2.85 |
According to the palatability test, the prepared isatis root granules have better palatability through optimizing the preparation process, and the compliance of veterinary medicines is obviously improved.
Claims (6)
1. The preparation process of the green granules is characterized by comprising the following steps of:
(1) Pretreatment of medicinal materials: chopping 6 parts by weight of isatis root and 9 parts by weight of dyers woad leaf for later use;
(2) Leaching:
decocting and leaching with water: mixing the pretreated radix Isatidis and folium Isatidis, adding 5-8 times of water, adding buffer solution to adjust pH to 7.2-8.0, decocting for 2 times each for 1 hr, mixing decoctions, and filtering to obtain decoction filtrate;
percolating and leaching: collecting the residue after decoction, pulverizing the residue again, placing in a percolator, adding 50% ethanol, adding alkyl glucoside, soaking for 12-18 hr, percolating, collecting 3/4 primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to obtain percolate I; adding 70% ethanol into the percolator, soaking for 8-15 hr, percolating, collecting 3/4 of primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to obtain percolate I; mixing the decoction filtrate, the percolate I and the percolate I to obtain a leaching solution;
(3) Separating and concentrating: centrifuging to remove precipitate in the leaching solution, and concentrating to obtain extract;
(4) Granulating: cooling the extract, adding excipient and flavoring agent, and granulating.
2. The process for preparing the isatis root granules according to claim 1, wherein the buffer solution in the water decoction leaching of the step (2) is phosphate buffer solution, and the ph=7.5-7.8.
3. The process for preparing isatis root granules according to claim 1, wherein the alkyl glucoside is used in an amount of 1/15 to 1/50 of the weight of isatis root and dyers woad leaf.
4. The process for preparing the green sheet particles according to claim 1, wherein the step (3) is: removing precipitate in the leaching solution in the step (2) by centrifugation, taking supernatant, and concentrating to obtain extract with density of 1.1-1.3 at normal pressure.
5. The process for preparing the isatis root granules according to claim 1, characterized in that the excipient is beta-cyclodextrin and the flavoring agent is sucrose.
6. The process for preparing the green sheet particles according to claim 1, comprising the steps of:
(1) Pretreatment of medicinal materials: 6 parts of isatis root and 9 parts of dyers woad leaf, and cutting up for later use;
(2) Leaching:
decocting and leaching with water: mixing the pretreated radix Isatidis and folium Isatidis, adding 5-8 times of water, adding phosphate buffer solution to adjust pH to 7.5-7.8, decocting for 2 times each for 1 hr, mixing decoctions, and filtering to obtain decoction filtrate;
percolating and leaching: collecting the decocted medicinal residues, pulverizing the medicinal residues again, placing into a percolator, adding 50% ethanol, adding 0.3-1 weight part of alkyl glucoside, soaking for 12-18 hr, percolating, collecting 3/4 primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to obtain percolate I; adding 70% ethanol into the percolator, soaking for 8-15 hr, percolating, collecting 3/4 of primary percolate, concentrating the secondary percolate at low temperature, and mixing with the primary percolate to obtain percolate I; mixing the decoction filtrate, the percolate I and the percolate I to obtain a leaching solution;
(3) Separating and concentrating: removing precipitate in the leaching solution in the step (2) by centrifugation, taking supernatant, and concentrating to obtain extract with density of 1.2 at normal pressure;
(4) Granulating: and cooling the extract, adding the crushed beta-cyclodextrin and sucrose, and granulating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210779299.XA CN115177679B (en) | 2022-07-01 | 2022-07-01 | Preparation technology of veterinary isatis root granules for improving content and palatability of active ingredients |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210779299.XA CN115177679B (en) | 2022-07-01 | 2022-07-01 | Preparation technology of veterinary isatis root granules for improving content and palatability of active ingredients |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115177679A CN115177679A (en) | 2022-10-14 |
| CN115177679B true CN115177679B (en) | 2023-05-09 |
Family
ID=83514643
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210779299.XA Active CN115177679B (en) | 2022-07-01 | 2022-07-01 | Preparation technology of veterinary isatis root granules for improving content and palatability of active ingredients |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115177679B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107898876A (en) * | 2017-12-19 | 2018-04-13 | 江西纵横生物科技有限公司 | Eutherapeutic plate green grass or young crops particle and preparation method thereof |
| CN108186706A (en) * | 2018-02-28 | 2018-06-22 | 山东信合生物制药有限公司 | A kind of isatis root-folium isatidis grain for livestock and preparation method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102727545B (en) * | 2012-06-30 | 2014-09-10 | 郑州百瑞动物药业有限公司 | Preparation method of indigowoad root and indigowoad leaf particles used for livestock and poultry |
| CN107998158A (en) * | 2017-12-19 | 2018-05-08 | 江西纵横生物科技有限公司 | High plate green grass or young crops particle of active constituent content and preparation method thereof |
| CN113713004A (en) * | 2021-09-16 | 2021-11-30 | 哈尔滨绿达生动物药业有限公司 | Radix isatidis granules and preparation method thereof |
-
2022
- 2022-07-01 CN CN202210779299.XA patent/CN115177679B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107898876A (en) * | 2017-12-19 | 2018-04-13 | 江西纵横生物科技有限公司 | Eutherapeutic plate green grass or young crops particle and preparation method thereof |
| CN108186706A (en) * | 2018-02-28 | 2018-06-22 | 山东信合生物制药有限公司 | A kind of isatis root-folium isatidis grain for livestock and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115177679A (en) | 2022-10-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107648326B (en) | Preparation method of amaranth yellow dysentery-stopping oral liquid for livestock | |
| CN114601842A (en) | Application of pulsatilla saponin refined extract in medicine for treating piglet diarrhea | |
| CN102318746B (en) | Animal health care product for bacterial livestock and poultry disease | |
| CN112587595B (en) | Traditional Chinese medicine composition for clearing heat and relieving exterior syndrome and preparation method thereof | |
| CN107184702B (en) | Traditional Chinese medicine composition for preventing and treating proventriculitis and myogastritis of poultry and preparation method thereof | |
| CN115177679B (en) | Preparation technology of veterinary isatis root granules for improving content and palatability of active ingredients | |
| CN110025657B (en) | Bacteriostatic traditional Chinese medicine composition, bacteriostatic traditional Chinese medicine preparation, and preparation method and application thereof | |
| CN111358850B (en) | Traditional Chinese medicine composition for preventing and treating chicken colibacillosis and preparation method thereof | |
| CN114304429A (en) | Traditional Chinese medicine composite probiotic feed additive for broiler chickens and preparation method thereof | |
| CN113521262A (en) | Lysozyme preparation with anti-inflammatory effect | |
| CN102416050B (en) | Veterinary medicinal preparation for treating colibacillosis and preparation method thereof | |
| CN109568430B (en) | Preparation method and application of immunopotentiator | |
| CN1927272A (en) | Medicament for animals with heat clearing and detoxicating, detumescence and obstruction removing functions and preparing method thereof | |
| CN115721677B (en) | Traditional Chinese medicine composition for preventing and treating calf bacterial diarrhea, and preparation method and application thereof | |
| CN115715783B (en) | Traditional Chinese medicine composition for preventing and treating white diarrhea of pigs and preparation method thereof | |
| CN111544570B (en) | Antiviral preparation added with antibacterial peptide and preparation method thereof | |
| CN113694096B (en) | Licorice extract and preparation method and application thereof | |
| CN103356739B (en) | A kind of Shuanghuanglian oral liquid for livestock and extracting method thereof | |
| CN115607614B (en) | Traditional Chinese medicine composition for treating colibacillosis of livestock and poultry and preparation method thereof | |
| CN102600210A (en) | Compound dextromethorphan hydrobromide syrup and preparation method for same | |
| CN103494913B (en) | Preparation method of compound radix astragali immune activation injection | |
| CN109731044B (en) | Traditional Chinese medicine compound granule for treating intestinal infection of poultry and preparation method thereof | |
| CN106619767A (en) | Medicine composition for treating dairy cow mastitis as well as preparation method and application thereof | |
| CN101606964A (en) | A kind of compound Chinese medicinal preparation for animals | |
| KR100839099B1 (en) | Composition comprising an extract of galla rhois and moutan cortex radicis for preventing and treating the porcine epidemic diarrhea virus(pedv) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A preparation process for improving the content and palatability of active ingredients in animal use Banqing granules Effective date of registration: 20231011 Granted publication date: 20230509 Pledgee: Postal Savings Bank of China Limited Yishui County sub branch Pledgor: Shandong Qi Kang Bio Technology Co.,Ltd. Registration number: Y2023980060903 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |