CN104126733A - Method for fermenting and producing feed additives using red-knees herbs and cassava residues as raw materials - Google Patents
Method for fermenting and producing feed additives using red-knees herbs and cassava residues as raw materials Download PDFInfo
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- CN104126733A CN104126733A CN201410367606.9A CN201410367606A CN104126733A CN 104126733 A CN104126733 A CN 104126733A CN 201410367606 A CN201410367606 A CN 201410367606A CN 104126733 A CN104126733 A CN 104126733A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a method for fermenting and producing feed additives using red-knees herbs and cassava residues as raw materials. According to the method, the cassava residues which belong to industrial waste are used as main raw materials, the red-knees herbs are matched, the cassava residues and the red-knees herbs are fermented in a synchronous saccharification and fermentation manner, the technology is simple, the manipulation strength is reduced, the efficiency is improved, the production cost can be effectively reduced, waste is changed into treasures, and the industrial production is facilitated. The protein content of the feed additives obtained by the method is more than 14.98%, the leaching efficiency of flavone is more than 18%, the feed additives are easy to digest and absorb by animals, the palatability of the feed additives is good, the organism immunity of the animals can be effectively improved, and the meat quality is improved.
Description
Technical field
The invention belongs to microorganism fermenting and producing field, particularly a kind of method of utilizing polygonum flaccidum and manioc waste to produce feed addictive for fermenting raw materials.
Background technology
Polygonum flaccidum (PolygonumhydropiperL) is the annual herb plant that belongs to Polygonum (Polygonaceae) polygonaceae (PolygonumL), extensively distribute in China, be a kind of abundant information, be easy to the cheap Chinese herbal medicine obtaining.It has good development prospect at aspects such as botanical pesticide, medicine, veterinary drug and food additives.Research to polygonum flaccidum at present and application mainly concentrate on: (1), as the ministerial drug of enterogastritis Chinese patent drug 'Fengliaochangweikang' ', is a kind of market medicine of maturation; (2) in distiller's yeast, add as auxiliary material, strengthen saccharification and the enzymolysis performance of distiller's yeast; (3) in all feeds additive as auxiliary material, to strengthen the growth promotion of feed, effect of anti-disease; (4) extract the main component of polygonum flaccidum, study its effect such as antibacterial, pest-resistant, anti-oxidant.Polygonum flaccidum, as very unique effectively antibacterial, the anti-oxidant Chinese herbal medicine of one, has the unrivaled advantage of other Chinese herbal medicines aspect feed addictive.
Flavones is a kind of important active components of plants, in plant, extensively exists.Research shows, flavones has high antioxygenic property, and the flavones content of vegetation water extract and its DPPH remove ability high-positive correlation.Polygonum flaccidum is rich in flavonoids composition, up to 4.79%, comprises the flavone components such as rutin, Quercetin, Hyperoside, rhamnose, Kaempferol.This has also given polygonum flaccidum anti-oxidation efficacy, can protect body, improves immunity, maintains the metabolic balance of body.In the time making feed addictive with it, can effectively prevent and alleviate the bad condition of noxious material and raising to the body injury of livestock and poultry, improve the speed of growth and dressing percentage, improve meat.
Manioc waste (Cassava pulp) is a kind of trade waste, originates from tapioca industry.More than 1,000 ten thousand tons, cassava is produced in the whole nation per year, and the manioc waste that tapioca industry produces approximately has 300,000 tons of left and right in dry.If a large amount of manioc wastes can not effectively be processed, will inevitably cause environmental pollution.In fact manioc waste, because water content is higher, is easy to grow microorganism, and inner cyanogen glycoside material is easily converted into the extremely toxic substances such as hydrogen cyanide, contaminated soil and atmosphere.The main component of manioc waste is cellulose, hemicellulose and starch, the protein (4% left and right) of small part.Because the content of the nutriments such as fat and protein is lower, manioc waste is not a kind of desirable feed or feed addictive.Therefore, the reasonable conversion to manioc waste and processing just seem particularly important.
Polygonum flaccidum and manioc waste all receive researcher's concern always as the prospect of feed and feed addictive.The one of Chinese patent CN102228152A using polygonum flaccidum as auxiliary material, extracts mixed plant active ingredient, and this extract, as pig feed additive, has disease-resistant growth-promoting effect.Chinese patent CN101385803A utilizes the one of polygonum flaccidum as auxiliary material, obtains compound Chinese herb medicament by lixiviate, is used for the treatment of livestock and poultry diarrhea and has good curative effect.But, Chinese patent CN102228152A and CN101385803A are all the extracts active ingredients of carrying out polygonum flaccidum and other Chinese herbal medicines under hot conditions, and inevitably, some sensitive compositions will lose, the operation of these two patents is very complicated simultaneously, and production cost is higher.
Summary of the invention
Primary and foremost purpose of the present invention is that the shortcoming that overcomes prior art, with not enough, provides a kind of method of utilizing polygonum flaccidum and manioc waste to produce feed addictive for fermenting raw materials.
Another object of the present invention is to provide the feed addictive obtaining by said method.
A further object of the present invention is the application of the feed addictive that provides described.
Object of the present invention is achieved through the following technical solutions: a kind of method of utilizing polygonum flaccidum and manioc waste to produce feed addictive for fermenting raw materials, comprises the steps:
(1) processing of raw material: pulverize manioc waste, obtain cassava ground-slag; Pulverize polygonum flaccidum, obtain polygonum flaccidum powder;
(2) preparation of fermentation medium A: preparation fermentation medium A, every liter of fermentation medium A contains following composition: cassava ground-slag 40～150g, polygonum flaccidum powder 7～33.3g, diammonium hydrogen phosphate 5.5～24g, corn steep liquor 0.3g, surplus is water, pH value is 4～5; Then sterilizing, cooling;
(3) diastatic fermentation: add amylase, carbohydrase and the cellulase of filtration sterilization in the sterilization fermentation culture medium A obtaining toward step (2), obtain fermentation medium B; S. cervisiae liquid after activation is inoculated in fermentation medium B to simultaneous saccharification and fermentation;
(4) zymotic fluid step (3) being obtained is dry, obtains feed addictive;
Cassava ground-slag described in step (1) is preferably the cassava ground-slag that can cross 40 mesh sieves;
Polygonum flaccidum powder described in step (1) is preferably the polygonum flaccidum powder that can cross 200 mesh sieves;
The condition of the sterilizing described in step (2) is preferably 115～121 DEG C of sterilizing 15～30min;
Diastatic addition described in step (3) is that every liter of sterilization fermentation culture medium A adds 6400～36000U amylase; Be preferably every liter of sterilization fermentation culture medium A and add 8000～36000U amylase;
The letter AMS heatproof SC of the preferred Novi of described amylase type;
The addition of the carbohydrase described in step (3) is that every liter of sterilization fermentation culture medium A adds 6400～36000U carbohydrase; Be preferably every liter of sterilization fermentation culture medium A and add 8000～36000U amylase;
The letter Amylase AG300L of the preferred Novi of described carbohydrase;
The addition of the cellulase described in step (3) is that every liter of sterilization fermentation culture medium A adds 90～375FPU cellulase; Be preferably every liter of sterilization fermentation culture medium A and add 190～275FPU cellulase;
The letter Celluclast1.5L of the preferred Novi of described cellulase;
S. cervisiae liquid after activation described in step (3) preferably prepares as follows: the S. cervisiae of freezing preservation is inoculated on solid medium, obtains first order seed; The single colony inoculation obtaining, to shaken cultivation in fluid nutrient medium, is obtained to secondary seed; Secondary seed is inoculated to shaken cultivation in fluid nutrient medium, obtain three grades of seeds, be the S. cervisiae liquid after activation;
Described solid medium is preferably yeast extract powder peptone glucose (YPD) agar medium;
Described fluid nutrient medium is preferably yeast extract powder peptone glucose (YPD) culture medium;
S. cervisiae liquid after described activation more preferably prepares as follows: the S. cervisiae of freezing preservation is lined on the inclined-plane that yeast extract powder peptone glucose agar medium makes to 30 DEG C of static cultivation 24h; By the single colony inoculation obtaining, to yeast extract powder peptone dextrose culture-medium, 30 DEG C, 180rpm are cultivated 12h, obtain secondary seed; Be that percent by volume 10% is inoculated in yeast extract powder peptone glucose (YPD) culture medium by secondary seed by inoculum concentration, 30 DEG C, 180rpm are cultivated 12h, obtain the S. cervisiae liquid after activation;
Described yeast extract powder peptone glucose (YPD) culture medium composed as follows: 20g/L glucose, 10g/L yeast extract powder, 5g/L peptone;
Described yeast extract powder peptone glucose (YPD) agar medium composed as follows: 20g/L glucose, 10g/L yeast extract powder, 5g/L peptone, 20g/L agar;
Described saccharomyces cerevisiae is preferably saccharomyces cerevisiae GIM2.121;
The volume of the inoculation described in step (3) is 2～20% of fermentation medium B volume; Be preferably 6～12%;
The temperature of the simultaneous saccharification and fermentation described in step (3) is preferably 28～37 DEG C; More preferably 28～32 DEG C; Most preferably be 30 DEG C;
The time of the simultaneous saccharification and fermentation described in step (3) is preferably 48～120h; More preferably 96～120h;
What step (4) was described is dried as dry or the freeze drying of spraying;
A kind of feed addictive, obtains by said method;
The protein content of described feed addictive is more than 14.98%, and flavones leaching rate is more than 18%;
Described feed addictive not only contains active dry yeasr, also contains polygonum flaccidum flavones extract, can directly be used as the feed addictive of bird.
The present invention has following advantage and effect with respect to prior art:
(1) technique of the present invention is simple, and the abundant raw material of use and cheapness are produced the feed addictive obtaining, polygonum flaccidum active ingredient extraction rate and protein content significantly promote, and are are easily digested and assimilated by animal, good palatability, can effectively improve the immunity of organisms of animal, improve meat.
(2) use trade waste manioc waste to ferment in simultaneous saccharification and fermentation mode for primary raw material, technique is simple, and manipulation strength declines, and efficiency improves, and can effectively reduce production costs, and turns waste into wealth, and is conducive to suitability for industrialized production.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Embodiment 1
(1) yeast starter liquid is prepared: by the saccharomyces cerevisiae of-80 DEG C of preservations (Saccharomyces Cerevisiae GIM2.121, microorganism fungus kind preservation center, Guangdong Province), line on the test tube slant that YPD Agr culture medium makes 30 DEG C of static cultivation 24h; By the single colony inoculation obtaining, to YPD fluid nutrient medium, 30 DEG C, 180rpm are cultivated 12h, obtain secondary seed; Be that percent by volume 10% is inoculated in YPD fluid nutrient medium by secondary seed by inoculum concentration, 30 DEG C, 180rpm are cultivated 12h, and (cell number reaches 10 to obtain yeast starter liquid
8individual/mL).The composition of YPD culture medium: glucose 20g/L, dusty yeast 10g/L, peptone 5g/L; , add 20g/L agar when on the inclined-plane; The initial pH nature of culture medium.
(2) fermentation medium is prepared and sterilizing: manioc waste is crossed 40 mesh sieves after pulverizing, and obtains cassava ground-slag; Polygonum flaccidum is crossed 200 mesh sieves after pulverizing, and obtains polygonum flaccidum powder.Manioc waste 40g/L, polygonum flaccidum 33.3g/L, supplement nitrogenous source (NH
4)
2hPO
4to concentration be 15.5g/L, add corn steep liquor (Aladdin, Cas66071-94-1, lower with) to concentration be 0.3g/L, the effect of corn steep liquor is microelement-supplementing, surplus is water, with 1M hydrochloric acid solution adjust pH be 4～5, obtain fermentation medium.15ml fermentation medium is placed in 150mL conical flask, 8 layers of gauze sealing.115 DEG C, 15min sterilizing.
(3) enzyme liquid is prepared and sterilizing: cellulase, amylase and carbohydrase are diluted to respectively desired concn, for subsequent use after 0.22 μ m membrane filtration degerming.
(4) simultaneous saccharification and fermentation: the volume of fermentation medium is 15mL, under sterile working, add 2.86FPU cellulase (Celluclast1.5L, Novi's letter, Denmark), 120U amylase (the letter AMS heatproof SC of Novi type), 120U carbohydrase (the letter Amylase AG300L of Novi).Then the yeast starter liquid that adds step (1) to obtain, inoculum concentration is percent by volume 6%.30 DEG C, 180rpm shaken cultivation 48h.
(5) dry: the zymotic fluid freeze drying that step (4) is obtained, obtains feed addictive.
(6) detect (same under detection method):
Spectra photometric method that 1. assay of zymotic fluid flavones adopts according to Wu Liangliang etc. (Wu Liangliang, Shi Xueping, Zhang Weiming. Chinese prickly ash Determination of Total Flavonoids method research [J]. food industry science and technology, 2010 (10): 372-374.) measure; The mensuration of polygonum flaccidum general flavone according to the method for Zhang Guoying paper (Zhang Guoying. the extraction of polygonum flaccidum main component, separate and identify [D]. Nanjing Forestry University, 2004.) measure.Calculate flavones leaching rate: flavones leaching rate=zymotic fluid flavones content/polygonum flaccidum general flavone * 100%.
2. use Kjeldahl nitrogen determination protein content.
Testing result is: flavones leaching rate is 27.56%, and protein content is 14.98%.
Embodiment 2
(1) yeast starter liquid is prepared: with embodiment step (1).
(2) fermentation medium is prepared and sterilizing: manioc waste is crossed 40 mesh sieves after pulverizing, and obtains cassava ground-slag; Polygonum flaccidum is crossed 200 mesh sieves after pulverizing, and obtains polygonum flaccidum powder.Manioc waste 60g/L, polygonum flaccidum 30g/L, supplement nitrogenous source (NH
4)
2hPO
4to concentration be 5.5g/L, adding corn steep liquor to concentration is 0.3g/L, with microelement-supplementing, surplus is water, with 1M hydrochloric acid solution adjust pH be 4～5, be placed in 150mL conical flask, 8 layers of gauze seal.115 DEG C, 15min sterilizing.
(3) enzyme liquid is prepared and sterilizing: cellulase, amylase and carbohydrase are diluted to respectively desired concn, for subsequent use after 0.22 μ m membrane filtration degerming.
(4) simultaneous saccharification and fermentation: the volume of zymotic fluid is 20mL, under sterile working, adds 4.23FPU cellulase, 288U amylase, 192U carbohydrase.The inoculum concentration of yeast starter liquid is percent by volume 10%.30 DEG C, 180rpm shaken cultivation 96h.
(5) dry: the zymotic fluid freeze drying that step (4) is obtained, obtains feed addictive.After testing, flavones leaching rate is 19.69%, and protein content is 16.40%.
Embodiment 3
(1) yeast starter liquid is prepared: with embodiment step (1).
(2) fermentation medium is prepared and sterilizing: manioc waste is crossed 40 mesh sieves after pulverizing, and obtains cassava ground-slag; Polygonum flaccidum is crossed 200 mesh sieves after pulverizing, and obtains polygonum flaccidum powder.Manioc waste 150g/L, polygonum flaccidum 7g/L, supplement nitrogenous source (NH
4)
2hPO
4to concentration be 24g/L, adding corn steep liquor to concentration is 0.3g/L, with microelement-supplementing, surplus is water, with 1M hydrochloric acid solution adjust pH be 4～5, be placed in 150mL conical flask, 8 layers of gauze seal.115 DEG C, 15min sterilizing.
(3) enzyme liquid is prepared and sterilizing: cellulase, amylase and carbohydrase are diluted to respectively desired concn, for subsequent use after 0.22 μ m membrane filtration degerming.
(4) simultaneous saccharification and fermentation: the volume of fermentation is 20mL, under sterile working, adds 5.48FPU cellulase, 720U amylase, 720U carbohydrase.The inoculum concentration of yeast starter liquid is percent by volume 10%.30 DEG C, 180rpm shaken cultivation 96h.
(5) dry: the zymotic fluid freeze drying that step (4) is obtained, obtains feed addictive.After testing, flavones leaching rate is 66.92%, and protein content is 16.33%.
Embodiment 4
(1) yeast starter liquid is prepared: with embodiment step (1).
(2) fermentation medium is prepared and sterilizing: manioc waste is crossed 40 mesh sieves after pulverizing, and obtains cassava ground-slag; Polygonum flaccidum is crossed 200 mesh sieves after pulverizing, and obtains polygonum flaccidum powder.Manioc waste 150g/L, polygonum flaccidum 7g/L, supplement nitrogenous source (NH
4)
2hPO
4to concentration be 24g/L, adding corn steep liquor to concentration is 0.3g/L, with microelement-supplementing, surplus is water, with 1M hydrochloric acid solution adjust pH be 4～5, be placed in 150mL conical flask, 8 layers of gauze seal.115 DEG C, 15min sterilizing.
(3) enzyme liquid is prepared and sterilizing: cellulase, amylase and carbohydrase are diluted to respectively desired concn, for subsequent use after 0.22 μ m membrane filtration degerming.
(4) simultaneous saccharification and fermentation: the volume of fermentation is 20mL, under sterile working, adds 5.48FPU cellulase, 600U amylase, 600U carbohydrase.The inoculum concentration of yeast starter liquid is percent by volume 12%.30 DEG C, 180rpm shaken cultivation 120h.
(5) dry: the zymotic fluid freeze drying that step (4) is obtained, obtains feed addictive.After testing, flavones leaching rate is 91.94%, and protein content is 20.46%.
Comparative example 1
(1) yeast starter liquid is prepared: with embodiment 1 step (1).
(2) fermentation medium is prepared and sterilizing: with embodiment 4 steps (2).
(3) enzyme liquid is prepared and sterilizing: amylase is diluted to desired concn, for subsequent use after 0.22 μ m membrane filtration degerming.
(4) simultaneous saccharification and fermentation: the volume of fermentation is 20mL, under sterile working, adds 600U amylase.Inoculum concentration is percent by volume 12%.30 DEG C, 180rpm shaken cultivation 120h.
(5) dry: the zymotic fluid freeze drying that step (4) is obtained, obtains feed addictive.After testing, protein content is 12.56%.
Comparative example 2
(1) yeast starter liquid is prepared: with embodiment 1 step (1).
(2) fermentation medium is prepared and sterilizing: with embodiment 4 steps (2).
(3) enzyme liquid is prepared and sterilizing: carbohydrase is diluted to desired concn, for subsequent use after 0.22 μ m membrane filtration degerming.
(4) simultaneous saccharification and fermentation: the volume of fermentation is 20mL, under sterile working, adds 600U carbohydrase.Inoculum concentration is percent by volume 12%.30 DEG C, 180rpm shaken cultivation 120h.
(5) dry: the zymotic fluid freeze drying that step (4) is obtained, obtains feed addictive.After testing, protein content is 13.22%.
Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under Spirit Essence of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.
Claims (10)
1. a method of utilizing polygonum flaccidum and manioc waste to produce feed addictive for fermenting raw materials, is characterized in that comprising the steps:
(1) processing of raw material: pulverize manioc waste, obtain cassava ground-slag; Pulverize polygonum flaccidum, obtain polygonum flaccidum powder;
(2) preparation of fermentation medium A: preparation fermentation medium A, every liter of fermentation medium A contains following composition: cassava ground-slag 40～150g, polygonum flaccidum powder 7～33.3g, diammonium hydrogen phosphate 5.5～24g, corn steep liquor 0.3g, surplus is water, pH value is 4～5; Then sterilizing, cooling;
(3) diastatic fermentation: add amylase, carbohydrase and the cellulase of filtration sterilization in the sterilization fermentation culture medium A obtaining toward step (2), obtain fermentation medium B; S. cervisiae liquid after activation is inoculated in fermentation medium B to simultaneous saccharification and fermentation;
(4) zymotic fluid step (3) being obtained is dry, obtains feed addictive.
2. the method for utilizing polygonum flaccidum and manioc waste to produce feed addictive for fermenting raw materials according to claim 1, is characterized in that:
Diastatic addition described in step (3) is that every liter of sterilization fermentation culture medium A adds 6400～36000U amylase;
The addition of the carbohydrase described in step (3) is that every liter of sterilization fermentation culture medium A adds 6400～36000U carbohydrase;
The addition of the cellulase described in step (3) is that every liter of sterilization fermentation culture medium A adds 90～375FPU cellulase.
3. the method for utilizing polygonum flaccidum and manioc waste to produce feed addictive for fermenting raw materials according to claim 2, is characterized in that:
Described diastatic addition is that every liter of sterilization fermentation culture medium A adds 8000～36000U amylase;
The addition of described carbohydrase is that every liter of sterilization fermentation culture medium A adds 8000～36000U amylase;
The addition of described cellulase is that every liter of sterilization fermentation culture medium A adds 190～275FPU cellulase.
4. the method for utilizing polygonum flaccidum and manioc waste to produce feed addictive for fermenting raw materials according to claim 1, is characterized in that:
Cassava ground-slag described in step (1) is the cassava ground-slag that can cross 40 mesh sieves;
Polygonum flaccidum powder described in step (1) is the polygonum flaccidum powder that can cross 200 mesh sieves;
The condition of the sterilizing described in step (2) is 115～121 DEG C of sterilizing 15～30min;
The volume of the inoculation described in step (3) is 2～20% of fermentation medium B volume;
The temperature of the simultaneous saccharification and fermentation described in step (3) is 28～37 DEG C;
The time of the simultaneous saccharification and fermentation described in step (3) is 48～120h;
What step (4) was described is dried as dry or the freeze drying of spraying.
5. the method for utilizing polygonum flaccidum and manioc waste to produce feed addictive for fermenting raw materials according to claim 1, it is characterized in that: the S. cervisiae liquid after the activation described in step (3) prepares as follows: the S. cervisiae of freezing preservation is inoculated on solid medium, obtains first order seed; The single colony inoculation obtaining, to shaken cultivation in fluid nutrient medium, is obtained to secondary seed; Secondary seed is inoculated to shaken cultivation in fluid nutrient medium, obtain three grades of seeds, be the S. cervisiae liquid after activation.
6. the method for utilizing polygonum flaccidum and manioc waste to produce feed addictive for fermenting raw materials according to claim 5, is characterized in that: described solid medium is yeast extract powder peptone glucose agar medium;
Described fluid nutrient medium is yeast extract powder peptone dextrose culture-medium.
7. the method for utilizing polygonum flaccidum and manioc waste to produce feed addictive for fermenting raw materials according to claim 5, it is characterized in that: the S. cervisiae liquid after described activation prepares as follows: the S. cervisiae of freezing preservation is lined on the inclined-plane that yeast extract powder peptone glucose agar medium makes to 30 DEG C of static cultivation 24h; By the single colony inoculation obtaining, to yeast extract powder peptone dextrose culture-medium, 30 DEG C, 180rpm are cultivated 12h, obtain secondary seed; Be that percent by volume 10% is inoculated in yeast extract powder peptone dextrose culture-medium by secondary seed by inoculum concentration, 30 DEG C, 180rpm are cultivated 12h, obtain the S. cervisiae liquid after activation.
8. a feed addictive, obtains by the method described in claim 1～7 any one.
9. feed addictive according to claim 8, is characterized in that: the protein content of described feed addictive is more than 14.98%, and flavones leaching rate is more than 18%.
10. the application of the feed addictive described in claim 8 or 9, is characterized in that: the feed addictive that described feed addictive is directly used as to bird.
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| CN104938768A (en) * | 2015-07-13 | 2015-09-30 | 北京英惠尔生物技术有限公司 | Fermentation method for fermenting liquid fodder |
| CN107173556A (en) * | 2017-05-04 | 2017-09-19 | 凤台硕力畜禽养殖有限公司 | A kind of processing method for making an addition to manioc waste in sheep feed |
| CN107460135A (en) * | 2017-09-14 | 2017-12-12 | 湖北金悦农产品开发有限公司 | Yeast zymotechnique and yeast tunning |
| CN108185160A (en) * | 2017-12-04 | 2018-06-22 | 杨忠华 | A kind of preparation method of cattle feed |
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| 刘琨等: "工业糖化酶固态发酵木薯渣制取单细胞蛋白饲料的研究", 《高校化学工程学报》 * |
| 沈慧: "自制糖化饲料喂牛", 《农民科技培训》 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104938768A (en) * | 2015-07-13 | 2015-09-30 | 北京英惠尔生物技术有限公司 | Fermentation method for fermenting liquid fodder |
| CN107173556A (en) * | 2017-05-04 | 2017-09-19 | 凤台硕力畜禽养殖有限公司 | A kind of processing method for making an addition to manioc waste in sheep feed |
| CN107460135A (en) * | 2017-09-14 | 2017-12-12 | 湖北金悦农产品开发有限公司 | Yeast zymotechnique and yeast tunning |
| CN108185160A (en) * | 2017-12-04 | 2018-06-22 | 杨忠华 | A kind of preparation method of cattle feed |
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| CN104126733B (en) | 2016-05-04 |
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