CN104126569A - Ginkgo biloba pollen fresh-keeping method - Google Patents
Ginkgo biloba pollen fresh-keeping method Download PDFInfo
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- CN104126569A CN104126569A CN201410346475.6A CN201410346475A CN104126569A CN 104126569 A CN104126569 A CN 104126569A CN 201410346475 A CN201410346475 A CN 201410346475A CN 104126569 A CN104126569 A CN 104126569A
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- ginkgo biloba
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Abstract
The invention discloses a ginkgo biloba pollen fresh-keeping method. The invention adopts a low-temperature refrigeration and a silica gel drying method for preservation of the collected ginkgo biloba pollen to effectively prolong the preservation time of pollen, and solve the technical problem of short pollen preservation time in artificial pollen collection and artificial pollination in ginkgo biloba nucleus application base with intensive management, so as to improve seed output and benefit in ginkgo biloba nucleus application cultivation. The method uses the characteristic that low-temperature drying treatment can prolong the life of ginkgo biloba pollen to prolongs the preservation time of ginkgo biloba pollen. The method for preservation of ginkgo grafted pollen has the advantages of simple operation and feasibility.
Description
Technical field
The invention belongs to the technical field that arboreal growth is grown and bred, particularly a kind of about the fresh-keeping method of gingko pollen.
Background technology
Ginkgo (Ginkgo biloba L.) is the Relict Plant of quaternary glacier, is the Ye Guocai excellent rare tree species of holding concurrently.Conduct ginkgo the first big country in the world of China, has the more than 90% of world's Ginkgo Germplasm Resource, and the cultivation history that can investigate in China is for thousand.To be chosen as the possibility of China " state tree " very large because having high economy, ecology and cultureal value for ginkgo.Ginkgo dioecism, mainly by wind pollination, owing to there being the different ripe phenomenon of dioecism, brings difficulty to ginkgo spontaneous pollination.The ginkgo core of intensive management is to ensure good quality and high output with base, conventionally artificial collection pollen, artificial pollination.And this mode mainly exists following outstanding problem in actual production: (1) gingko pollen maturation is is easily scattered and disappeared afterwards, be difficult to collect.(2) gingko pollen 15 days left and right pollen viabilities under normal temperature condition drop to below 10%, lose germinating capacity.
The feature that the present invention utilizes low temperature drying processing can extend the gingko pollen life-span, adopts deepfreeze and silica dehydrator mode to preserve the gingko pollen of collecting, and has effectively extended pollen fresh keeping time.Yet there are no the report of gingko pollen preservation technique, the present invention proposes under such technical background.
Summary of the invention
The object of the invention is; conventionally difficult collection of pollen there will be while preservation for gingko pollen; the phenomenon of vitality degradation under normal temperature condition, these phenomenons have had a strong impact on gingko pollen fresh keeping time, thereby have restricted seed production and the benefit of ginkgo core with cultivation.In order to address the above problem, the present invention has adopted following technical scheme:
(1) selection of pollen: mid-April, in the time of the micro-Huang of ginkgo microstrobilus, set the raw male flower branch of collection 3-5 from being greater than 50 years breeder mother.
(2) collection of pollen: in the indoor wide-mouth beaker that ginkgo spray oblique cutting is entered to fill clear water, immersed depth 20-30cm, the template of beaker below place mat one 50 × 50cm size, guarantee microstrobilus be positioned at template directly over, to accept the pollen being scattered.After 7-10 days, when microstrobilus cracking, pollen dispersed falls behind, and collects and puts in vitro, seals with absorbent cotton.
(3) storage of pollen: the test tube that fills gingko pollen is put into the drier that silica gel is housed, then drier is put into temperature and be the refrigerator of 2 DEG C and preserve.
The invention has the beneficial effects as follows:
(1) before gingko pollen maturation, choose the uncracked microstrobilus of micro-Huang, effectively solve gingko pollen maturation easy lost problem afterwards.
(2) in the indoor wide-mouth beaker that ginkgo spray oblique cutting is entered to fill clear water, and below beaker place mat template, guarantee microstrobilus be positioned at template directly over, can make so ripe pollen dispersed drop on template, be convenient to collect the pollen that is scattered.
(3) put into by containing polliniferous test tube the drier that silica gel is housed, then put into temperature and be the refrigerator of 2 DEG C and preserve, the drier that silica gel is housed has ensured the drying condition of pollen storage, and the refrigerator of 2 DEG C provides the cryogenic conditions of pollen storage.
Utilize said method to preserve gingko pollen, preserve more than half a year, above pollen germination rate can reach 70%-95%, good refreshing effect, easy and simple to handle, with low cost, extend the storage time of gingko pollen.
Embodiment
Embodiment 1
Application this method is carried out in Ginkgo Germplasm Resource garden, Shandong Agricultural University's forestry proving ground, Tai’an, Shandong Province, processing is collected, preserved to gingko pollen, to extend gingko pollen fresh keeping time.Gingko pollen preservation method implementation step is as follows:
1. the selection of pollen
Mid-April, in the time of the micro-Huang of ginkgo microstrobilus, set the raw male flower branch of collection 3-5 from being greater than 50 years breeder mother.
2. the collection of pollen
In the indoor wide-mouth beaker that ginkgo spray oblique cutting is entered to fill clear water, immersed depth 20-30cm, the template of beaker below place mat one 50 × 50cm size, guarantee microstrobilus be positioned at template directly over, to accept the pollen being scattered.After 7-10 days, when microstrobilus cracking, pollen dispersed falls behind, and collects and puts in vitro, seals with absorbent cotton.
3. the storage of pollen
The test tube that fills gingko pollen is put into the drier that silica gel is housed, then drier is put into temperature and be the refrigerator of 2 DEG C and preserve.
The present invention is not limited to above embodiment, as long as taked the method for above selection, collection and storage pollen in gingko pollen preservation technique problem, all will fall within the scope of protection of the present invention.
Claims (4)
1. a gingko pollen preservation method is characterised in that and adopts deepfreeze and silica dehydrator mode to preserve the gingko pollen of collecting, and the method comprises the following steps:
(1) selection of pollen: mid-April, in the time of the micro-Huang of ginkgo microstrobilus, set the raw male flower branch of collection 3-5 from being greater than 50 years breeder mother;
(2) collection of pollen: in the indoor wide-mouth beaker that ginkgo spray oblique cutting is entered to fill clear water, immersed depth 20-30cm, the template of beaker below place mat one 50 × 50cm size, guarantee microstrobilus be positioned at template directly over, to accept the pollen being scattered, after 7-10 days, when microstrobilus cracking, pollen dispersed falls behind, collection is put in vitro, seals with absorbent cotton;
(3) storage of pollen: the test tube that fills gingko pollen is put into the drier that silica gel is housed, then drier is put into temperature and be the refrigerator of 2 DEG C and preserve.
2. a kind of gingko pollen preservation method according to claim 1, is characterized in that, the selection of the described pollen of step (1) method access time.
3. a kind of gingko pollen preservation method according to claim 1, is characterized in that, the selection of the described pollen collecting method of step (2).
4. a kind of gingko pollen preservation method according to claim 1, is characterized in that, the selection of the described pollen storage method of step (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410346475.6A CN104126569A (en) | 2014-07-18 | 2014-07-18 | Ginkgo biloba pollen fresh-keeping method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410346475.6A CN104126569A (en) | 2014-07-18 | 2014-07-18 | Ginkgo biloba pollen fresh-keeping method |
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| Publication Number | Publication Date |
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| CN104126569A true CN104126569A (en) | 2014-11-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410346475.6A Pending CN104126569A (en) | 2014-07-18 | 2014-07-18 | Ginkgo biloba pollen fresh-keeping method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106036361A (en) * | 2016-08-22 | 2016-10-26 | 文山苗乡三七科技有限公司 | Panax notoginseng pollen storage method |
| CN109287623A (en) * | 2018-10-18 | 2019-02-01 | 南京林业大学 | A kind of purple U.S. pomegranate pollen storage practice |
| CN109832262A (en) * | 2019-01-25 | 2019-06-04 | 袁隆平农业高科技股份有限公司 | Live plant store method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH01139510A (en) * | 1987-11-27 | 1989-06-01 | Gakken Co Ltd | Storage of pollen of japanese horseradish |
| CN1615689A (en) * | 2004-11-03 | 2005-05-18 | 廖旭辉 | Technology for storing Momordica grosvenori pollen |
| CN102613170A (en) * | 2012-03-14 | 2012-08-01 | 中国林业科学研究院林业研究所 | Cattleya pollen storage method |
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2014
- 2014-07-18 CN CN201410346475.6A patent/CN104126569A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01139510A (en) * | 1987-11-27 | 1989-06-01 | Gakken Co Ltd | Storage of pollen of japanese horseradish |
| CN1615689A (en) * | 2004-11-03 | 2005-05-18 | 廖旭辉 | Technology for storing Momordica grosvenori pollen |
| CN102613170A (en) * | 2012-03-14 | 2012-08-01 | 中国林业科学研究院林业研究所 | Cattleya pollen storage method |
Non-Patent Citations (5)
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| 唐登松等: "银杏人工授粉技术的研究", 《落叶果树》 * |
| 孙霞等: "银杏花粉生活力研究", 《果树科学》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106036361A (en) * | 2016-08-22 | 2016-10-26 | 文山苗乡三七科技有限公司 | Panax notoginseng pollen storage method |
| CN109287623A (en) * | 2018-10-18 | 2019-02-01 | 南京林业大学 | A kind of purple U.S. pomegranate pollen storage practice |
| CN109832262A (en) * | 2019-01-25 | 2019-06-04 | 袁隆平农业高科技股份有限公司 | Live plant store method |
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Application publication date: 20141105 |