CN104429786B - A kind of method of high eyebrow Parakmeria sexual propagation - Google Patents
A kind of method of high eyebrow Parakmeria sexual propagation Download PDFInfo
- Publication number
- CN104429786B CN104429786B CN201410845309.0A CN201410845309A CN104429786B CN 104429786 B CN104429786 B CN 104429786B CN 201410845309 A CN201410845309 A CN 201410845309A CN 104429786 B CN104429786 B CN 104429786B
- Authority
- CN
- China
- Prior art keywords
- seed
- sand
- parakmeria
- humidity
- sowing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
A kind of method that the invention discloses high eyebrow Parakmeria sexual propagation comprises the following steps: (1) gathers pollen and carries out artificial pollination；(2) collect and process seed；(3) sowing.The setting percentage using the technology of the present invention can reach 95%, and setting percentage is only 12% under natural conditions, sand of the present invention is used to hide the seed germination rate of method storage up to 80%, it is provided without Sha Zangfa only according to conventional the storage of seeds method storage, its seed germination rate is less than 15%, and seed germination rate is extremely low under natural conditions, only 2%.The present invention makes endangered rare tree high eyebrow Parakmeria achieve amount reproduction, for studying its endangered mechanism, searches out and is effectively protected and saves countermeasure and be significant.
Description
Technical field
The present invention relates to agriculture forestry planting field, a kind of method being specifically related to high eyebrow Parakmeria sexual propagation.
Background technology
High eyebrow Parakmeria is the evergreen of (Parakmeria omeiensis Cheng) Magnoliaceae (Magnoliaceae) Parakmeria Hu & Cheng (Parakmeria)Arbor, up to 25 meters, be the type sepecies of Parakmeria Hu & Cheng, Sichuan endemic species, country-level focused protection rare or endangered species, is also the national minimum population wild plant urgently saving protection.Field is only distributed in shoulder pole precipice one band of Mount Emei's height above sea level 2200 meters, and due to the fragility of living environment, particularly under the natural environment that the mankind and monkey group disturb, the impact being damaged is bigger, and current field only finds more than 50 strains.High eyebrow Parakmeria is dioecism, cross-pollinatd plant, and staminiferous plant is many under field conditions (factors), and female plant is few, and male flower is bloomed early than female flower.Female flower is bloomed for the first time and is concentrated between dusk 6:00-8:00, and under natural conditions, pollination difficulty, setting percentage is low, and seed quality is poor, and seed is difficult to germination and growth under field conditions (factors), almost loses the ability of self-reproduction.Therefore, it is desirable to protect, save, breed high eyebrow Parakmeria, just can only be by artificial propagation.
Summary of the invention
In view of above-mentioned weak point, a kind of method that it is an object of the invention to provide high eyebrow Parakmeria sexual propagation, the method is possible not only to improve the setting percentage of high eyebrow Parakmeria, it is also possible to improve seed germination rate, it is thus achieved that healthy and strong seedling.
In order to achieve the above object, present invention employs techniques below scheme:
A kind of method of high eyebrow Parakmeria sexual propagation, comprises the following steps:
(1) gather pollen and carry out artificial pollination: after staminiferous plant enters florescence, select the male flower that flower pesticide has just ftractureed, take out flower pesticide；After all tapels of female flower first switch on, its stigma is creamy white and secretes when having mucus, pollinates；
(2) collect and process seed: when peel is bronzing, get final product collecting fruit in JIUYUE, fruit dry in the sun is stripped seed in one week after peel splits, and wash the aril of redness by rubbing with the hands, after the surface of the seed is cleaned up, seed and sterilized sand are mixed, uses the method storage of husky Tibetan, and keep its humidity at 80-90%, preserve under the conditions of 4 DEG C, and periodically carry out observing its humidity；
(3) sowing: March next year is sowed, uses hole tray sowing, one, a cave, and peat soil and the perlite that sowing media uses mass ratio to be 7:3, using compound fertilizer as base fertilizer.
Described husky Tibetan method is particularly as follows: first to sand sterilization treatment 30 minutes under the conditions of 121 DEG C, it is cooled to room temperature, seed and sand are pressed the mixing of 1:2 mass ratio again, add water simultaneously, sand humidity is made to be maintained at 80%-90%, then the seed mixed homogeneously with sand is contained in one in the gauze bag of 75% ethanol sterilizing, preserve under the conditions of 4 DEG C, and make regular check on its humidity.
The invention provides the sexual reproduction method of a kind of high eyebrow Parakmeria, the method can significantly improve its setting percentage, and uses the fruit of the technology of the present invention acquisition, seed quality to be significantly better than the fruit under natural conditions, seed.The setting percentage using the technology of the present invention can reach 95%, and setting percentage is only 12% under natural conditions, sand of the present invention is used to hide the seed germination rate of method storage up to 80%, it is provided without Sha Zangfa only according to conventional the storage of seeds method storage, its seed germination rate is less than 15%, and seed germination rate is extremely low under natural conditions, only 2%.The present invention makes endangered rare tree high eyebrow Parakmeria achieve amount reproduction, for studying its endangered mechanism, searches out and is effectively protected and saves countermeasure and be significant.
Detailed description of the invention
We will the present invention is further illustrated in conjunction with specific embodiments below.
A kind of method of high eyebrow Parakmeria sexual propagation comprises the following steps:
(1) gather pollen and carry out artificial pollination: after high eyebrow Parakmeria staminiferous plant enters florescence, select the male flower that flower pesticide has just ftractureed, take out flower pesticide；After the high eyebrow all tapels of Parakmeria female flower first switch on, its stigma is creamy white and secretes when having mucus, pollinates；Through test, gathering after pollen carries out artificial pollination mode by the present invention, high eyebrow Parakmeria setting percentage reaches 95%.Finding in force, if the non-secreting mucus of the stigma of female flower is just pollinated, can affect Pollination Effect, ovule only a few is expanded, thus is reduced setting percentage.Therefore, need to observe the flowering dynamics of female flower in force, it is to avoid miss the Best Times of pollination always.
(2) collect and process seed: when peel is bronzing, get final product collecting fruit in JIUYUE, fruit dry in the sun is stripped seed in one week after peel splits, and wash the aril of redness by rubbing with the hands, after the surface of the seed is cleaned up, seed and sterilized sand are mixed, use the method storage of husky Tibetan, Sha Zangfa is particularly as follows: first to sand sterilization treatment 30 minutes under the conditions of 121 DEG C, it is cooled to room temperature, seed and sand are pressed the mixing of 1:2 mass ratio again, add water simultaneously, sand humidity is made to be maintained at 85%, then the seed mixed homogeneously with sand is contained in one in the gauze bag of 75% ethanol sterilizing, preserve under the conditions of 4 DEG C, and make regular check on its humidity.Finding in force, humidity all can affect seed vitality less than 80% or higher than 90%, if humidity is less than 80%, intraseminal moisture is very fast to external diffusion, is unfavorable for the after-ripening of seed, and seed germination rate is less than 40%；After more than 90%, seed can go mouldy.
(3) sowing: March next year is sowed, uses hole tray sowing, one, a cave, and peat soil and the perlite that sowing media uses mass ratio to be 7:3, using compound fertilizer (N:P:K mass ratio is as 15:10:10) as base fertilizer.Investigate its germination rate May, reach 80%.
Claims (1)
1. the method for a high eyebrow Parakmeria sexual propagation, it is characterised in that the method comprises the following steps:
(1) gather pollen and carry out artificial pollination: after staminiferous plant enters florescence, select the male flower that flower pesticide has just ftractureed, take out flower pesticide；After all tapels of female flower first switch on, its stigma is creamy white and secretes when having mucus, pollinates；
(2) collect and process seed: when peel is bronzing, get final product collecting fruit in JIUYUE, fruit dry in the sun is stripped seed in one week after peel splits, and wash the aril of redness by rubbing with the hands, after the surface of the seed is cleaned up, seed and sterilized sand are mixed, uses the method storage of husky Tibetan, and keep its humidity at 80-90%, preserve under the conditions of 4 DEG C, and periodically carry out observing its humidity；
(3) sowing: March next year is sowed, uses hole tray sowing, one, a cave, and peat soil and the perlite that sowing media uses mass ratio to be 7:3, using compound fertilizer as base fertilizer；
Described husky Tibetan method is: first to sand sterilization treatment 30 minutes under the conditions of 121 DEG C, it is cooled to room temperature, seed and sand are pressed the mixing of 1:2 mass ratio again, add water simultaneously, sand humidity is made to be maintained at 80%-90%, then the seed mixed homogeneously with sand is contained in one in the gauze bag of 75% ethanol sterilizing, preserve under the conditions of 4 DEG C, and make regular check on its humidity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410845309.0A CN104429786B (en) | 2014-12-31 | 2014-12-31 | A kind of method of high eyebrow Parakmeria sexual propagation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410845309.0A CN104429786B (en) | 2014-12-31 | 2014-12-31 | A kind of method of high eyebrow Parakmeria sexual propagation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104429786A CN104429786A (en) | 2015-03-25 |
| CN104429786B true CN104429786B (en) | 2016-08-31 |
Family
ID=52877149
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410845309.0A Active CN104429786B (en) | 2014-12-31 | 2014-12-31 | A kind of method of high eyebrow Parakmeria sexual propagation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104429786B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107409792A (en) * | 2017-06-28 | 2017-12-01 | 成都大学 | A kind of generative propagation research method of sargent magnolia bark |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101049087A (en) * | 2007-05-11 | 2007-10-10 | 中国科学院昆明植物研究所 | Method for breeding plant brand of 'Yujuan Mulan' |
| JP6034053B2 (en) * | 2012-04-26 | 2016-11-30 | トヨタ自動車株式会社 | Production method of total plant seeds |
| CN103168695B (en) * | 2013-04-15 | 2014-06-11 | 四川省自然资源科学研究院 | Tissue culture method of parakmeria omeiensis cheng |
-
2014
- 2014-12-31 CN CN201410845309.0A patent/CN104429786B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104429786A (en) | 2015-03-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105340747A (en) | Asexual rapid propagation method for radix glycyrrhizae | |
| Singh | Effect of IBA concentrations on the rooting of pomegranate (Punica granatum L.) cv. Ganesh hardwood cuttings under mist house condition | |
| CN101773032A (en) | Quick propagation seedling establishment method for bracken spore matrix | |
| Kapczyńska | Effect of bulb size on growth, flowering and bulb formation in lachenalia cultivars. | |
| CN103858771A (en) | Maize transgenic tissue culture seedling transplanting method | |
| CN106417014B (en) | A kind of big flower pattern pocket orchid breeding and cultural method | |
| US20190208789A1 (en) | Compositions and methods for enhancing plant growth | |
| CN109526298B (en) | Passion fruit seed treatment method and sowing and breeding method thereof | |
| CN104429786B (en) | A kind of method of high eyebrow Parakmeria sexual propagation | |
| CN109511534B (en) | Berry seed sowing and breeding method | |
| CN103975855A (en) | Haploid breeding method of dendrobium candidum | |
| CN106258079A (en) | A kind of Fructus actinidiae chinensis seedling breeding method | |
| Chohan et al. | Effect of sodium nitroprusside on morphological characters under chilling stress in chickpea (Cicer arietinum L.) | |
| CN103190400A (en) | New application of sodium butyrate for improving resistance of plant to powdery mildew | |
| Devecı et al. | The Effect of Water Deficit on some Physiological Properties of Abelmoschus esculentus (L.) Moench cv." Sultani" | |
| Lee et al. | Botanical and germinating characteristics of Miscanthus species native to Korea | |
| CN104255482A (en) | Method for quickly reproducing Koelreuteria paniculata by tissue culture | |
| Morinaga et al. | Effect of salt and water stress on fruit quality, physiological responses, macro-and micro-element contents in leaves of Satsuma mandarin trees under greenhouse conditions | |
| Sharma et al. | Impact of protective condition and media on growth of seedling in Guava cv. L-49 in north western-Haryana, India | |
| CN104823649A (en) | Method for improving germination rate of oriental blueberry seeds | |
| KR20160067439A (en) | Cultivation method of sinseng sprout and cultivation set for the same | |
| Peng-fei et al. | Apomixis and new selections of walnut | |
| CN104012382A (en) | Large-scale seedling culturing technology for rhododendron calophytum Franch | |
| Grzesik et al. | Quality of seeds as a key to commodity cultivation of Lavatera Thuringiaca L.–plants with high potential for multi directional use | |
| CN104521481A (en) | Strawberry planting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |