CN102613170A - Cattleya pollen storage method - Google Patents
Cattleya pollen storage method Download PDFInfo
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- CN102613170A CN102613170A CN2012100668697A CN201210066869A CN102613170A CN 102613170 A CN102613170 A CN 102613170A CN 2012100668697 A CN2012100668697 A CN 2012100668697A CN 201210066869 A CN201210066869 A CN 201210066869A CN 102613170 A CN102613170 A CN 102613170A
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- cattleya
- bowring cattleya
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Abstract
The invention discloses a Cattleya pollen storage method, which includes dry storage of Cattleya pollen at 4 DEG C and below or humid storage at -20 DEG C, preferably, dry storage at -20 DEG C. By the method, vitality of the Cattleya pollen can be maintained for more than 2 years, pollination effect is unaffected, and breeding is available all year round.
Description
Technical field
The present invention relates to a kind of pollen storage method, relate in particular to a kind of Bowring cattleya pollen storage method.
Background technology
Bowring cattleya belongs to the orchid family (family Orchideceae), tree orchid subfamily (subfamily Epidendroideae), tree orchid family (tribe Epidendreae), Lei Liya subtribe (subtribe Laeliinae), has another name called Jiade Li Yalan, blue type of the multiple stem property of growing nonparasitically upon another plant.Its pattern riot of color, that the flower type is very large is colourful, but Her breath is like the sweet odour of perfumes people, color are rich and varied, and the laudatory title of " king of cattleya " is arranged in the world.Originate in american torrid zone and subtropics, wherein Colombia is wild at most with Brazil, and states such as Colombia, Brazil, Costa Rica more are national flower with the Bowring cattleya.Bowring cattleya all has the production of scale property in Thailand, Singapore, Indonesia, Malaysia, Philippine and China Taiwan, and these areas become the main producing region of world's Bowring cattleya.
The Bowring cattleya initial species of report has 110 kinds (http://apps.kew.org/wcsp/home.do) approximately at present; But itself and relative genus Brassavola (white drag-line is blue), Laelia (the flower bud Leah is blue), Sophronitis (Suo Fu rowland) etc. have successfully carried out intergeneric cross; Bred a large amount of crossbreed, the Bowring cattleya of indication is all original seeds and the crossbreed of general reference Cattleya genus and other relevant relative genus at present, comprises that Bowring cattleya belongs to original seed and genus cross kind; Bowring cattleya relative genus and genus cross kind; Bowring cattleya belongs to the crossbreed with relative genus, and the crossbreed between the Bowring cattleya relative genus, has formed huge Bowring cattleya family kind.Because the various types of Bowring cattleya florescence is inconsistent, in the crossbreeding process, normal flowering date is not met, and need preserve the pollinium of gathering, and storage practice has material impact to pollen viability.
Because the pollen coated outside lipid membrane of orchid makes the pollen grain adhesion become pollinium, different storage practices is bigger to Bowring cattleya pollen effect of vigor.
In the prior art, be that normal temperature is preserved and 4 ℃ of moistening storages of low temperature to the store method of Bowring cattleya pollen, under these two kinds of store methods, the pollen devitalization is fast.In the crossbreeding process, if two kind florescences differed more than 1 month, conventional store method can't satisfy the breeding needs.
Summary of the invention
The purpose of this invention is to provide and a kind ofly can make the pollen vigor holding time long, and the pollination effect not have the Bowring cattleya pollen storage method that influences.
The objective of the invention is to realize through following technical scheme:
Bowring cattleya pollen storage method of the present invention, with Bowring cattleya pollen at dry storage below 4 ℃ or 4 ℃, or with Bowring cattleya pollen-20 ℃ of moistening storages.
Technical scheme by the invention described above provides can be found out; Bowring cattleya pollen storage method provided by the invention; Since with Bowring cattleya pollen in dry storage below 4 ℃ or 4 ℃ or-20 ℃ of moistening storages; The pollen vigor was preserved more than 2 years, and, made breeding to carry out throughout the year the not influence of pollination effect.
Embodiment
To do to describe in detail further to the embodiment of the invention below.
Bowring cattleya pollen storage method of the present invention, its preferable embodiment is:
With Bowring cattleya pollen at dry storage below 4 ℃ or 4 ℃, or with Bowring cattleya pollen-20 ℃ of moistening storages.
Can be with Bowring cattleya pollen at 4 ℃～-20 ℃ dry storages.
Preferably with Bowring cattleya pollen at-20 ℃ of dry storages.
Adopt the pollinium store method of this patent, the pollen vigor was preserved more than 2 years, and to the not influence of pollination effect, this provides technical support for effectively carrying out Bowring cattleya crossbreeding research.
Concrete experimental example:
Adopt two kinds of methods of moistening preservation and kept dry, respectively in 4 ℃ of refrigerations and-20 ℃ of chilled storages, totally 4 kinds of methods are preserved the Bowring cattleya polliniums, and experimental result is as shown in table 1.
The result shows: the pollen germination rate of 4 ℃ of moistening storages after 15 days descends very fast, is merely 33.6%, preserves after 30 days, and the germination rate of pollen drops to 3.5%, and along with the prolongation of storage time, pollen is devitalization gradually.And pollen germination rate descends slowlyer under other 3 kinds of holding conditions, preserves after 60 days, and the germination rate of pollen is still more than 56%; Preserve after 720 days; Pollen still has higher germination rate, and especially-20 under ℃ freeze drying condition, the germination rate of pollen is still up to 40.4%.Hybrid Bowring cattleya self-pollination test is shown pollinate with and 720 days pollen of dry storage moistening through 4 ℃ of dry storages ,-20 ℃, ripening rate is still up to more than 90%.
The germination rate (%) of hybrid Bowring cattleya pollen under the different holding conditions of table 1
In the table 1, different capitalizations and lowercase are represented difference extremely significantly (P＜0.01) and remarkable (P＜0.05) respectively after the data.
The present invention is through research moistening preservation of pollinium and kept dry (desiccant use silica gel); And two kinds of store methods preservation effect under 4 ℃ of refrigerations and-20 ℃ of chilled storage conditions respectively; The result shows: 4 ℃ of moistening storages are after 30 days, and the pollen vigor is lost basically.Other 3 kinds of method storage pollen pieces are after 720 days, and pollen still has higher germination rate, and especially-20 under ℃ freeze drying condition, the germination rate of pollen is still up to 40.4%.Wherein higher with 4 ℃ of dry storages ,-20 ℃ of dry storage pollen vigor, after pollinating, ripening rate is still up to more than 90%.
Therefore; In hybrid Bowring cattleya crossbreeding process; If run into flowering asynchronism or need the strange land pollination; Can pollinium elder generation drying be handled through 4 ℃ of deepfreezes again, perhaps directly pollinium carried out-20 ℃ of freezing preservations, all can make hybrid Bowring cattleya pollen in 1-2, keep higher vitality.
The above; Be merely the preferable embodiment of the present invention, but protection scope of the present invention is not limited thereto, any technical staff who is familiar with the present technique field is in the technical scope that the present invention discloses; The variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.
Claims (3)
1. a Bowring cattleya pollen storage method is characterized in that, with Bowring cattleya pollen at dry storage below 4 ℃ or 4 ℃, or with Bowring cattleya pollen-20 ℃ of moistening storages.
2. Bowring cattleya pollen storage method according to claim 1 is characterized in that, with Bowring cattleya pollen at 4 ℃～-20 ℃ dry storages.
3. Bowring cattleya pollen storage method according to claim 2 is characterized in that, with Bowring cattleya pollen at-20 ℃ of dry storages.
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| CN2012100668697A CN102613170A (en) | 2012-03-14 | 2012-03-14 | Cattleya pollen storage method |
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|---|---|---|---|
| CN2012100668697A CN102613170A (en) | 2012-03-14 | 2012-03-14 | Cattleya pollen storage method |
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| CN102613170A true CN102613170A (en) | 2012-08-01 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004753A (en) * | 2012-12-11 | 2013-04-03 | 浙江大学 | Long-time watermelon pollen preservation method and corresponding fruit setting promotion method by using early spring facility |
| CN103385238A (en) * | 2013-07-01 | 2013-11-13 | 镇江瑞繁农艺有限公司 | Method for keeping water lily pollen viability |
| CN104126569A (en) * | 2014-07-18 | 2014-11-05 | 山东农业大学 | Ginkgo biloba pollen fresh-keeping method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101129128A (en) * | 2007-09-27 | 2008-02-27 | 上海交通大学 | Cross breeding method for hippeastrum |
-
2012
- 2012-03-14 CN CN2012100668697A patent/CN102613170A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101129128A (en) * | 2007-09-27 | 2008-02-27 | 上海交通大学 | Cross breeding method for hippeastrum |
Non-Patent Citations (2)
| Title |
|---|
| 郑宝强，等: "卡特兰花粉萌发和花粉贮藏性研究", 《中国观赏园艺研究进展2011》, 18 August 2011 (2011-08-18) * |
| 郑宝强，等: "杂种卡特兰花粉萌发和花粉贮藏性研究", 《热带亚热带植物学报》, vol. 20, no. 1, 15 January 2012 (2012-01-15) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004753A (en) * | 2012-12-11 | 2013-04-03 | 浙江大学 | Long-time watermelon pollen preservation method and corresponding fruit setting promotion method by using early spring facility |
| CN103004753B (en) * | 2012-12-11 | 2014-12-24 | 浙江大学 | Long-time watermelon pollen preservation method and corresponding fruit setting promotion method by using early spring facility |
| CN103385238A (en) * | 2013-07-01 | 2013-11-13 | 镇江瑞繁农艺有限公司 | Method for keeping water lily pollen viability |
| CN104126569A (en) * | 2014-07-18 | 2014-11-05 | 山东农业大学 | Ginkgo biloba pollen fresh-keeping method |
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Inventor after: Wang Yan Inventor after: Zheng Baoqiang Inventor after: Zhou Jinchang Inventor after: Chen Zhenhuang Inventor after: Huang Zhenhong Inventor before: Wang Yan Inventor before: Zheng Baoqiang |
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Free format text: CORRECT: INVENTOR; FROM: WANG YAN ZHENG BAOQIANG TO: WANG YAN ZHENG BAOQIANG ZHOU JINCHANG CHEN ZHENHUANG HUANG ZHENHONG |
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Application publication date: 20120801 |