CN103385238A - Method for keeping water lily pollen viability - Google Patents
Method for keeping water lily pollen viability Download PDFInfo
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- CN103385238A CN103385238A CN2013102690863A CN201310269086A CN103385238A CN 103385238 A CN103385238 A CN 103385238A CN 2013102690863 A CN2013102690863 A CN 2013102690863A CN 201310269086 A CN201310269086 A CN 201310269086A CN 103385238 A CN103385238 A CN 103385238A
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- water lily
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- lily pollen
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Abstract
The invention discloses a method for keeping water lily pollen viability, which comprises the following steps: 1) drying the water lily pollen naturally for 39 min and packaging and sealing thereafter; 2) storing in a refrigerator at a temperature of -20 DEG C; 3) transferring water lily pollen to a refrigerator refrigerating chamber at a temperature of 4 DEG C for 4 hours for transition; 4) taking out the water lily pollen and then putting it into a container with filter paper saturated with water for 30 min in order to have the water lily pollen reabsorb air moisture to resume viability. In this way, the viability of the water lily pollen can be maintained over a month, and water lily pollination isn't influenced, which solves the problems of blossoming of flowers at different times and pollination in different places in the process of water lily cross breeding.
Description
Technical field
The present invention relates to the cross breeding method of a kind of water lily, especially keep the method for water lily pollen viability in not oversize time about one month, belong to ornamental plants crossbreeding technology field.
Background technology
Water lily be the Nymphaeceae Nymphaea (
Nymphaea) general term of plant, water lily is with its abundant pattern, holy and pure implied meaning and the cauline leaf strong adsorption capacity to nutrient rich in water and harmful substance, and is very popular, is widely used in water feature design.Be the natural florescence of various cold-resistant water lily kinds May to October, in the crossbreeding process, the situation that flowering asynchronism can't breeding often occurs, further formed the reproduction isolation.It is one of important channel that solves flowering asynchronism that the water lily pollen that gathers is preserved a period of time, but under general condition preserves water lily pollen, and its vitality there will be significantly decline, is difficult to be cross-breeding.
The principal element that affects pollen viability is temperature and humidity, and according to the influence factor of pollen viability, the storage practice of pollen roughly is summarized as following several: cold drying, vacuum storage method, Cryopreservation method and lyophilize and preserve method etc.Said method has his own strong points, can be according to the purpose employing of suiting measures to local conditions of storage while specifically applying.The Chinese invention patent application that is CN 101926323 A as publication number discloses Bowring cattleya Pollen preservation method,, with pollen dry storage under-20 ℃, can make Pollen Activity preserve more than 2 years.Publication number is that the Chinese invention patent of CN 102613170 A discloses a kind of oil palm pollen storage method for another example, and the fresh oil palm pollen that gathers is carried out drying after removing impurity, and the residual moisture content that makes oil palm pollen is 8-9%; Dried oil palm pollen is packed in cryovial and directly put into pre-freeze in liquid nitrogen and process, and then be moved in ultra low temperature freezer and preserve, the oil palm pollen of while thawing, ultralow temperature being preserved can thaw with the flushing of normal temperature running water.As seen, various plant pollen storage temperature and humidity and the method for thawing are not quite similar, but not yet see the method that keeps the water lily pollen viability about month in the time.
Summary of the invention
The object of the present invention is to provide a kind ofly can keep the method for pollen viability within least one month, and can not affect the Pollination Effect of water lily pollen.
The present invention is achieved by the following technical programs:
A kind of method that keeps the water lily pollen viability comprises the following steps:
1) collection of water lily pollen and drying
Gather mature water lily flower, stamen pollen is collected on template, the aluminium foil paper bag is put in natural seasoning after 25～35 minutes, then aluminium-foil paper is packaged in centrifuge tube and seals;
2) low temperature storage
The centrifuge tube that water lily pollen is housed is put into the freezer chamber of refrigerator chilled storage of-18～-22 ℃;
3) water lily pollen thaws and uses front alleviation transition
The centrifuge tube that water lily pollen is housed is transferred under the cold storage chamber of refrigerator of 3～5 ℃ of low temperature transition from the freezer chamber of refrigerator of-18～-22 ℃ 3.5～4.5 hours;
4) water lily pollen recovers vitality
Take out the centrifuge tube that water lily pollen is housed of having alleviated transition from cold storage chamber of refrigerator, first take out the aluminium foil paper bag in centrifuge tube, then open the aluminium foil paper bag, take out water lily pollen, put it into again in the container of full of water filter paper 25～35 minutes, allow water lily pollen reuptake air moisture and recover vitality, then take the field pollination.
The present invention provides a kind of science, simple water lily pollen store method to the Nymphaea kind, can make the water lily Pollen Activity keep more than 1 month, and, on the not impact of water lily Pollination Effect, solved the problem such as flowering asynchronism and strange land pollination in water lily crossbreeding process.
Advantage and disadvantage of the present invention, will make an explanation by the non-limitative illustration of following preferred embodiment, and these embodiment only provide as an example.
Embodiment
Below in conjunction with the embodiment of the water lily kind that is called " luxurious ", the invention will be further described.
The present embodiment comprises the following steps:
1) collection of water lily pollen and drying
Gather mature tens water lily flowers, with tweezers, stamen pollen is collected on template, the aluminium foil paper bag is put in natural seasoning after 30 minutes, then aluminium-foil paper is packaged in centrifuge tube and seals.
2) low temperature storage
The centrifuge tube that water lily pollen is housed is put into the freezer chamber of refrigerator chilled storage of-20 ℃;
3) water lily pollen thaws and uses front alleviation transition
The centrifuge tube that water lily pollen is housed is transferred under the cold storage chamber of refrigerator of 4 ℃ of low temperature transition from the freezer chamber of refrigerator of-20 ℃ 4 hours;
4) water lily pollen recovers vitality
Take out the centrifuge tube that water lily pollen is housed of having alleviated transition from cold storage chamber of refrigerator, first take out the aluminium foil paper bag in centrifuge tube, then open the aluminium foil paper bag, take out water lily pollen, put it into again in the container of full of water filter paper 30 minutes, allow water lily pollen reuptake air moisture and recover vitality, then take the field pollination.
Following table is the water lily kind of " luxurious " by name of the preservation of employing the inventive method and the water lily inter breed crossing of " Peter " by name, and the ripening rate result under the difference holding times contrasts.
| Holding time (my god) | " Peter " and " luxurious " results of hybridization |
| 0 | 93±4.2% |
| 15 | 84±2.8% |
| 30 | 86±4.7% |
| 60 | 58±2.1% |
As can be seen from the table, the water lily pollen that adopts the inventive method to preserve 30 days has still kept the vitality of pollen, and after preserving 30 days " luxurious " kinds and " Peter " inter breed crossing, the water lily ripening rate still has 86 ± 4.7%.
In addition to the implementation, the present invention can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent deformation one-tenth, all drop in the protection domain of requirement of the present invention.
Claims (1)
1. method that keeps the water lily pollen viability is characterized in that: comprise the following steps:
1) collection of water lily pollen and drying
Gather mature water lily flower, stamen pollen is collected on template, the aluminium foil paper bag is put in natural seasoning after 25～35 minutes, then aluminium-foil paper is packaged in centrifuge tube and seals;
2) low temperature storage
The centrifuge tube that water lily pollen is housed is put into the freezer chamber of refrigerator chilled storage of-18～-22 ℃;
3) water lily pollen thaws and uses front alleviation transition
The centrifuge tube that water lily pollen is housed is transferred under the cold storage chamber of refrigerator of 3～5 ℃ of low temperature transition from the freezer chamber of refrigerator of-18～-22 ℃ 3.5～4.5 hours;
4) water lily pollen recovers vitality
Take out the centrifuge tube that water lily pollen is housed of having alleviated transition from cold storage chamber of refrigerator, first take out the aluminium foil paper bag in centrifuge tube, then open the aluminium foil paper bag, take out water lily pollen, put it into again in the container of full of water filter paper 25～35 minutes, allow water lily pollen reuptake air moisture and recover vitality, then take the field pollination.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2013102690863A CN103385238A (en) | 2013-07-01 | 2013-07-01 | Method for keeping water lily pollen viability |
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| CN2013102690863A CN103385238A (en) | 2013-07-01 | 2013-07-01 | Method for keeping water lily pollen viability |
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| CN103385238A true CN103385238A (en) | 2013-11-13 |
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| CN2013102690863A Pending CN103385238A (en) | 2013-07-01 | 2013-07-01 | Method for keeping water lily pollen viability |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107864750A (en) * | 2016-09-23 | 2018-04-03 | 四川种都高科种业有限公司 | A kind of tomato pollen collection, the method to maintain vigour |
| CN108739800A (en) * | 2018-06-26 | 2018-11-06 | 陕西省西安植物园 | A kind of method of strongly fragrant Lonicera periclymenum in vitro pollen short term storage |
| CN109168644A (en) * | 2018-08-30 | 2019-01-11 | 江苏沿江地区农业科学研究所 | A kind of bedleaf cherry plum pollen collection store method |
| CN109938012A (en) * | 2019-04-28 | 2019-06-28 | 南京林业大学 | A kind of efficient method collected and save perfume lotus flower anther and pollen |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06157201A (en) * | 1992-11-30 | 1994-06-03 | Japan Tobacco Inc | Storage of pollen of cruciferous plant |
| CN1762202A (en) * | 2005-11-08 | 2006-04-26 | 中国科学院植物研究所 | A kind of sunflower powder method in service life that prolongs |
| JP2009040703A (en) * | 2007-08-07 | 2009-02-26 | National Agriculture & Food Research Organization | Method for preserving pollen |
| CN102144632A (en) * | 2011-01-30 | 2011-08-10 | 广西壮族自治区林业科学研究院 | Method for storing and unfreezing pinus massoniana pollen |
| CN102613170A (en) * | 2012-03-14 | 2012-08-01 | 中国林业科学研究院林业研究所 | Cattleya pollen storage method |
-
2013
- 2013-07-01 CN CN2013102690863A patent/CN103385238A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06157201A (en) * | 1992-11-30 | 1994-06-03 | Japan Tobacco Inc | Storage of pollen of cruciferous plant |
| CN1762202A (en) * | 2005-11-08 | 2006-04-26 | 中国科学院植物研究所 | A kind of sunflower powder method in service life that prolongs |
| JP2009040703A (en) * | 2007-08-07 | 2009-02-26 | National Agriculture & Food Research Organization | Method for preserving pollen |
| CN102144632A (en) * | 2011-01-30 | 2011-08-10 | 广西壮族自治区林业科学研究院 | Method for storing and unfreezing pinus massoniana pollen |
| CN102613170A (en) * | 2012-03-14 | 2012-08-01 | 中国林业科学研究院林业研究所 | Cattleya pollen storage method |
Non-Patent Citations (2)
| Title |
|---|
| MASAKAZU KASUMI等: "Effectiveness of Cold Stored Pollen on Seed Formation in East Indian Lotus(Nelumbo nucifera Gaertn.)", 《JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE》, vol. 69, no. 6, 31 December 2000 (2000-12-31), pages 732 - 735 * |
| 李晨: "荷花花粉形态学及生活力研究", 《河南农业大学硕士学位论文》, 8 December 2012 (2012-12-08) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107864750A (en) * | 2016-09-23 | 2018-04-03 | 四川种都高科种业有限公司 | A kind of tomato pollen collection, the method to maintain vigour |
| CN108739800A (en) * | 2018-06-26 | 2018-11-06 | 陕西省西安植物园 | A kind of method of strongly fragrant Lonicera periclymenum in vitro pollen short term storage |
| CN109168644A (en) * | 2018-08-30 | 2019-01-11 | 江苏沿江地区农业科学研究所 | A kind of bedleaf cherry plum pollen collection store method |
| CN109938012A (en) * | 2019-04-28 | 2019-06-28 | 南京林业大学 | A kind of efficient method collected and save perfume lotus flower anther and pollen |
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Application publication date: 20131113 |