CN101637124A - Pre-culture medium for storing Chinese yam idioplasm by embedding vitrifaction at ultralow temperature and cultivation method thereof - Google Patents
Pre-culture medium for storing Chinese yam idioplasm by embedding vitrifaction at ultralow temperature and cultivation method thereof Download PDFInfo
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- CN101637124A CN101637124A CN200910066047A CN200910066047A CN101637124A CN 101637124 A CN101637124 A CN 101637124A CN 200910066047 A CN200910066047 A CN 200910066047A CN 200910066047 A CN200910066047 A CN 200910066047A CN 101637124 A CN101637124 A CN 101637124A
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- chinese yam
- culture medium
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- vitrifaction
- idioplasm
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Abstract
The invention discloses a pre-culture medium for storing Chinese yam idioplasm by embedding vitrifaction at ultralow temperature and a cultivation method thereof, aiming at providing a pre-culture medium for storing Chinese yam idioplasm by embedding vitrifaction at ultralow temperature and a cultivation method thereof so as to be capable of applying the embedding vitrifaction cryopreservation technology in the conservation of the Chinese yam idioplasm. The invention has the technical scheme that the pre-culture medium contains an MS basic culture medium which is added with any of the two permeable substances: (1) 0.1mol/L of glycerol and 0.2mol/L of sucrose; and (2) 0.2mol/L of sucrose. Compared with the prior art, the invention has the remarkable advantages of simple operation, long conservation time and good genetic stability of regeneration plants after storage, thus being applied to in vitro conservation of Chinese yam.
Description
Technical field:
The present invention relates to a kind of pre-culture medium and cultural method thereof of quality saving technology, particularly a kind of storing Chinese yam idioplasm by embedding vitrifaction at ultralow temperature.
Background technology:
Chinese yam is a kind of food that is loved by the people, and is again the very high and widely used traditional Chinese medicine of a kind of medical value, Huai Chinese yam especially, and its medical value is higher.Yet, the preservation technology of Chinese yam idioplasm is still continued to use traditional method, many precious germ plasm resources are lost, and can not adapt to the requirement of now growing demand.Ultralow temperature preservation technology is a kind of quality saving method of present long shelf-life, stay in grade, application maturation.The embedding vitrifying method ultralow temperature that occur the nineties in 20th century is preserved technology, combines the advantage of vitrifying method and encapsulation dehydration method, and has overcome embedding dry method complex operation and the formation of the inside and outside ice crystal of cell and the toxicity of vitrifying method.At present in application of succeeding such as apple, peppermint, strawberry, persimmon, horseradish, sweet potato, garlics.Yet this kind method is practiced by successive transfer culture, low temperature and is thawed after seedling, pre-cultivation, embedding, loading and dehydration processing, liquid nitrogen are preserved, preserved and wash and link such as the cultivation of regenerating is formed, use in the preservation of this method of overtesting at Chinese yam idioplasm and fail, one of its reason is that pre-culture medium is unsuitable for Chinese yam idioplasm after deliberation.
Summary of the invention:
The purpose of this invention is to provide a kind of pre-culture medium and cultural method thereof that embedding vitrifying method ultralow temperature preservation technology can be applied in the storing Chinese yam idioplasm by embedding vitrifaction at ultralow temperature of Chinese yam idioplasm preservation.Technical solution of the present invention is, a kind of pre-culture medium of embedding vitrification cryopreservation of yam, it includes the MS minimal medium, it is characterized in that: also be added with any of following two kinds of permeate substances in MS: 1. 0.1mol/L glycerine and 0.2mol/L sucrose; 2. 0.2mol/L sucrose.A kind of pre-culture medium of the embedding vitrification cryopreservation of yam made from claim 1 carries out pre-incubated method to Chinese yam idioplasm, comprise the Chinese yam test-tube plantlet behind the cold acclimation is cut into stem with bud, it is characterized in that: the band bud Chinese yam stem section that will cut is put into pre-culture medium 4 ℃ of following constant cultivations 3-5 days.That the present invention has compared with the prior art is simple to operate, the holding time long and preserve the remarkable advantage of back good genetic stability of regeneration plants.
Embodiment:
Pre-culture medium of the present invention is MS+0.2mol/L sucrose+0.1mol/L glycerine or MS+0.2mol/L sucrose.
Pre-cultural method of the present invention is, the Huai Chinese yam behind the cold acclimation (general Chinese yam also can) test-tube plantlet is cut into the stem with bud about 1cm, and the pre-culture medium of putting into the invention described above is 4 ℃ of constant cultivations 3-5 days down.
By to the holding time and the discovering of genetic stability of preserving the back regeneration plant: relative survival rate and the survival rate of holding time to material all do not influence; Regeneration plant is preserved seedling with normal temperature and is compared, and variation all appears in content, SOD, POD, PPO and the diastatic isodynamic enzyme zymogram of plant height, the number of blade, bud number, stipes length, chlorophyll and soluble protein and RAPD band.
Claims (2)
1, a kind of pre-culture medium of storing Chinese yam idioplasm by embedding vitrifaction at ultralow temperature, it includes the MS minimal medium, it is characterized in that: also be added with any of following two kinds of permeate substances in MS: 1. 0.1mol/L glycerine and 0.2mol/L sucrose; 2. 0.2mol/L sucrose.
2, a kind of pre-culture medium of the embedding vitrification cryopreservation of yam made from claim 1 carries out pre-incubated method to Chinese yam idioplasm, comprise the Chinese yam test-tube plantlet behind the cold acclimation is cut into stem with bud, it is characterized in that: the band bud Chinese yam stem section that will cut is put into pre-culture medium 4 ℃ of following constant cultivations 3-5 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910066047A CN101637124A (en) | 2009-09-03 | 2009-09-03 | Pre-culture medium for storing Chinese yam idioplasm by embedding vitrifaction at ultralow temperature and cultivation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910066047A CN101637124A (en) | 2009-09-03 | 2009-09-03 | Pre-culture medium for storing Chinese yam idioplasm by embedding vitrifaction at ultralow temperature and cultivation method thereof |
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| CN101637124A true CN101637124A (en) | 2010-02-03 |
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| CN200910066047A Pending CN101637124A (en) | 2009-09-03 | 2009-09-03 | Pre-culture medium for storing Chinese yam idioplasm by embedding vitrifaction at ultralow temperature and cultivation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103278361A (en) * | 2013-05-16 | 2013-09-04 | 青岛科技大学 | Low-temperature embedding method of polymer thin film |
| CN113060392A (en) * | 2021-03-16 | 2021-07-02 | 天益食品（徐州）有限公司 | Burdock of adjustable interval of depositing is deposited and is used sterilizer |
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2009
- 2009-09-03 CN CN200910066047A patent/CN101637124A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103278361A (en) * | 2013-05-16 | 2013-09-04 | 青岛科技大学 | Low-temperature embedding method of polymer thin film |
| CN103278361B (en) * | 2013-05-16 | 2015-06-17 | 青岛科技大学 | Low-temperature embedding method of polymer thin film |
| CN113060392A (en) * | 2021-03-16 | 2021-07-02 | 天益食品（徐州）有限公司 | Burdock of adjustable interval of depositing is deposited and is used sterilizer |
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Open date: 20100203 |