CN1762202A - A kind of sunflower powder method in service life that prolongs - Google Patents
A kind of sunflower powder method in service life that prolongs Download PDFInfo
- Publication number
- CN1762202A CN1762202A CN 200510117728 CN200510117728A CN1762202A CN 1762202 A CN1762202 A CN 1762202A CN 200510117728 CN200510117728 CN 200510117728 CN 200510117728 A CN200510117728 A CN 200510117728A CN 1762202 A CN1762202 A CN 1762202A
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- China
- Prior art keywords
- pollen
- sunflower powder
- sunflower
- service life
- prolongs
- Prior art date
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- 238000000034 method Methods 0.000 title claims abstract description 32
- 241000208818 Helianthus Species 0.000 title claims abstract description 28
- 235000003222 Helianthus annuus Nutrition 0.000 title claims abstract description 28
- 239000000843 powder Substances 0.000 title claims abstract description 24
- 238000003860 storage Methods 0.000 claims abstract description 23
- 238000001035 drying Methods 0.000 claims abstract description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 239000000741 silica gel Substances 0.000 claims description 5
- 229910002027 silica gel Inorganic materials 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 4
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 claims description 4
- 239000002274 desiccant Substances 0.000 claims description 3
- 239000000292 calcium oxide Substances 0.000 claims description 2
- 235000012255 calcium oxide Nutrition 0.000 claims description 2
- 230000005070 ripening Effects 0.000 abstract description 13
- 238000009395 breeding Methods 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 6
- 238000004321 preservation Methods 0.000 abstract description 5
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 abstract description 4
- 230000002068 genetic effect Effects 0.000 abstract description 3
- 230000014759 maintenance of location Effects 0.000 abstract description 3
- 108010016634 Seed Storage Proteins Proteins 0.000 abstract description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000010257 thawing Methods 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000036119 Frailty Diseases 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of sunflower powder method in service life that prolongs.This method is that sunflower powder is carried out the ultralow temperature storage after drying, it is thawed with the method that progressively heats up during use again, makes the life-span of sunflower powder obtain prolonging.This method has solved sunflower preservation problem of pollen in flowering asynchronism and the strange land pollinating process in breeding process.With the easy row, effectively of this method caching sunflower powder, only need very little investment, can be with quite a large amount of pollen storages in a very little space, and ripening rate can reach 12-68%.The present invention helps breeding man to solve the difficult problem of seed storage requirement than large space, will provide more genetic resources for breeding work, has important practical value.
Description
Technical field
The present invention relates to a kind of sunflower powder method in service life that prolongs.
Background technology
Sunflower is the fourth-largest oil crop in the world, in its " three are " breeding work, needs to utilize the pollen in strange land sometimes, just might run into the different problem of parent's flowering time.Only break the isolation of the hybrid strain time and space, just can have increased access to the chance of new varieties, this just makes the work of stocking of preciousness, rare genetic germplasm seem and is even more important.Mature pollen is comprising whole hereditary information of plant, so the storage of sunflower powder parent's flowering asynchronism and artificial pollination when solving hybridization, germ plasm resource preservation etc. all have important and practical meanings.
At present, the research of relevant sunflower powder storage property is still few, Roath equals report in 1988: pollen was preserved in refrigerator 1 year, ripening rate only 4%, and storage 1 year in refrigerator-freezer then fully can not the solid (storages of W.W.Roath sunflower powder, Dang Jiuzhan translates, external agronomy-oil crop, 1995,1:24-25).Other has report, and only be 5 days the service life of the sunflower powder of freezing storage, seek out higher ripening rate, and pollen leaves 4 ℃ of times in the refrigerator in and is no more than 3 days at most.
Summary of the invention
The purpose of this invention is to provide a kind of sunflower powder method in service life that prolongs.
The method in prolongation sunflower powder provided by the present invention service life is that sunflower powder is carried out the ultralow temperature storage after drying, it is thawed with the method that progressively heats up during use again, makes the life-span of sunflower powder obtain prolonging.
In said method, pollen is carried out dry equipment can be and be equipped with silica gel, NaNO
2, CaCl
2, LiCl, NaHSO
4H
2O, (NH
4)
2SO
4, Na
2HPO
412H
2The drier of desiccant such as O or quicklime is preserved with the vaseline sealing.The different dry drying prescription control effect difference that wets is preferably the drier that is equipped with silica gel; For making pollen obtain better storage effect, make the water content of sunflower powder reach 6-8% after the described drying; The temperature of ultralow temperature storage is preferably-80 ℃ to-196 ℃; The method that pollen is thawed is preferably: will go to-20 ℃ through the pollen of ultralow temperature storage earlier and keep 1-12 days down, and go to 4 ℃ again and kept 1-30 days down.
The invention provides a kind of sunflower powder method in service life that prolongs.This method has solved sunflower preservation problem of pollen in flowering asynchronism and the strange land pollinating process in breeding process.Easily capable, effective with this method caching sunflower powder, only need very little investment, can be with quite a large amount of pollen storages in a very little space, ripening rate still can reach 12-68%.The invention solves the difficult problem of seed storage requirement, will provide more genetic resources, have important practical value for breeding work than large space.
The present invention will be further described below in conjunction with specific embodiment.
Embodiment
Method therefor is conventional method if no special instructions among the following embodiment.
The storage of embodiment 1, sunflower powder and thawing
One, the collection of pollen and vigor detect
Gather sunflower powder about 10:00 the fine morning in the sunflower full-bloom stage, detect after pollen is dyeed with TTC.What dyeing took on a red color is the stronger pollen of vigor, and pale red is the more weak pollen of vigor, and undyed is debility pollen.Testing result shows that the pollen of being gathered has higher vigor.Divide equally the EP pipe packing of back with 10mL, every pipe can be adorned the pollen of about 3g, open-ended.
Two, the drying of pollen
Open-ended in the step 1, EP pipe that pollen is housed are placed the drier that is placed with silica gel, after making pollen water content reach 7%, with the taking-up of obturaging of the EP mouth of pipe, can change desiccant at any time according to the size of dry pollen amount during this time, making water content in 12 hours with baking in 100 ℃ baking oven serves as that zero pollen is contrast.The result toasts in 100 ℃ baking oven and made water content in 12 hours is that the ripening rate of zero pollen is 0%, shows that relative moisture is the key factor that influences pollen viability.Within the specific limits, relative moisture and pollen storage life-span are negative correlation, and may to be that relative moisture is low can make the pollen metabolism be suppressed to its reason, make the reduced activity of enzyme, reduce respiration, thereby the pollen storage life-span is prolonged, preserve but bone-dry condition also is unfavorable for pollen.
Three, the storage of pollen
The EP pipe that step 2 is filled dry pollen places liquid nitrogen (196 ℃) preservation, preserves all the year round as if needing, and only pollen need be transferred in-80 ℃ of refrigerators to get final product after the liquid nitrogen preservation.Mensuration is with the ripening rate of the method storage pollen after 1 year, 2 years and 3 years, and ripening rate is respectively 12-68%, 11-56% and 8-44%, shows with this storage practice to make pollen all keep higher activity in a long time.
Four, thaw handle and different defreezing method to the influence of pollen vigor
Pollen by after taking out in liquid nitrogen or-80 ℃ of refrigerators, was placed pollen respectively 1,6,12 day in-20 ℃ of refrigerators earlier after ultralow temperature storage, went to place respectively in 4 ℃ of refrigerators again and thawed in 1,15,30 day.Carry out field trial after thawing to measure its ripening rate, the ripening rate statistics is as shown in table 1, show that the ripening rate with the method storage and the pollen through thawing can reach 12-68%, compare that the ripening rate tool improves a lot with the pollen of preserving through conventional method (ripening rate only is 4%).
Table 1 ripening rate statistics (%)
| 1 | 62 | 68 | 12 |
| 6 | 66 | 47 | 23 |
| 12 | 55 | 52 | 21 |
Claims (6)
1, a kind of sunflower powder method in service life that prolongs is that sunflower powder is carried out the ultralow temperature storage after drying, it is thawed with the method that progressively heats up during use again, makes obtain prolonging the service life of sunflower powder.
2, method according to claim 1 is characterized in that: described pollen is carried out dry equipment for being equipped with silica gel, NaNO
2, CaCl
2, LiCl, NaHSO
4H
2O, (NH
4)
2SO
4, Na
2HPO
412H
2The drier of O or quicklime.
3, method according to claim 2 is characterized in that: described to be used for pollen is carried out dry desiccant be silica gel.
4, according to claim 1 or 2 or 3 described methods, it is characterized in that: the water content of described sunflower powder after drying is 6-8%.
5, according to claim 1 or 2 or 3 described methods, it is characterized in that: the temperature of described ultralow temperature storage is-80 ℃ to-196 ℃.
6, according to claim 1 or 2 or 3 described methods, it is characterized in that: the described method that pollen is thawed is: will go to-20 ℃ through the pollen of ultralow temperature storage earlier and keep 1-12 days down, and go to 4 ℃ again and kept 1-30 days down.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510117728 CN1762202A (en) | 2005-11-08 | 2005-11-08 | A kind of sunflower powder method in service life that prolongs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510117728 CN1762202A (en) | 2005-11-08 | 2005-11-08 | A kind of sunflower powder method in service life that prolongs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1762202A true CN1762202A (en) | 2006-04-26 |
Family
ID=36746682
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510117728 Pending CN1762202A (en) | 2005-11-08 | 2005-11-08 | A kind of sunflower powder method in service life that prolongs |
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| Country | Link |
|---|---|
| CN (1) | CN1762202A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101926323A (en) * | 2010-07-29 | 2010-12-29 | 中国热带农业科学院橡胶研究所 | Ultra-low temperature preservation method for oil palm pollen |
| CN102415370A (en) * | 2011-08-18 | 2012-04-18 | 湖南省蔬菜研究所 | Ultralow temperature preservation method for capsicum pollens |
| CN103385238A (en) * | 2013-07-01 | 2013-11-13 | 镇江瑞繁农艺有限公司 | Method for keeping water lily pollen viability |
| CN104705292A (en) * | 2015-03-31 | 2015-06-17 | 河北双星种业有限公司 | Ultra-low-temperature storage method for sunflower pollen |
| WO2019010360A1 (en) * | 2017-07-06 | 2019-01-10 | Accelerated Ag Technologies, Llc | Pollen preservation method |
-
2005
- 2005-11-08 CN CN 200510117728 patent/CN1762202A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101926323A (en) * | 2010-07-29 | 2010-12-29 | 中国热带农业科学院橡胶研究所 | Ultra-low temperature preservation method for oil palm pollen |
| CN101926323B (en) * | 2010-07-29 | 2013-06-12 | 中国热带农业科学院橡胶研究所 | Ultra-low temperature preservation method for oil palm pollen |
| CN102415370A (en) * | 2011-08-18 | 2012-04-18 | 湖南省蔬菜研究所 | Ultralow temperature preservation method for capsicum pollens |
| CN103385238A (en) * | 2013-07-01 | 2013-11-13 | 镇江瑞繁农艺有限公司 | Method for keeping water lily pollen viability |
| CN104705292A (en) * | 2015-03-31 | 2015-06-17 | 河北双星种业有限公司 | Ultra-low-temperature storage method for sunflower pollen |
| WO2019010360A1 (en) * | 2017-07-06 | 2019-01-10 | Accelerated Ag Technologies, Llc | Pollen preservation method |
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