CN104116803B - A kind of preparation method of the Chinese medicine microecological preparation preventing and treating duck pestilence - Google Patents

A kind of preparation method of the Chinese medicine microecological preparation preventing and treating duck pestilence Download PDF

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CN104116803B
CN104116803B CN201410350152.4A CN201410350152A CN104116803B CN 104116803 B CN104116803 B CN 104116803B CN 201410350152 A CN201410350152 A CN 201410350152A CN 104116803 B CN104116803 B CN 104116803B
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chinese medicine
duck
fermentation
duck pestilence
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CN104116803A (en
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郭庆
阮武营
王伟杰
杨涛
杜海燕
韩俊伟
黄岩
邹小娟
赵智灿
王海涛
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Guangzhou and Biotechnology Co., Ltd.
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郭庆
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

A kind of preparation method of the Chinese medicine microecological preparation preventing and treating duck pestilence, it is prepared by the following method, the intense fire that Flos Lonicerae, Ramulus Cinnamomi, Radix Isatidis, Lysimachia christinae Hance, Indigo Naturalis, Rhizoma Coptidis, the Radix Astragali, Herb Gynostemmae Pentaphylli, Radix Glycyrrhizae added water boils, slow fire insulation 30~60min, cold filtration, collect filtrate, add white sugar to boil, inoculating bacillus subtilis, lactobacillus and Zygosaccharomyces bailii bacterium after cooling down to room temperature through 3~7d fermentation gained microbial ecological agent, gained microbial ecological agent is drunk directly to duck after diluting in proportion.Chinese medicine microecological preparation of the present invention have profitable strain quantity in antiviral, inducement interferon, increase intestinal, improve immunologic function, strengthen duck body premunition, the effect such as without any side effects, and preparation method is simple, easy to use, noresidue, viable bacteria content are high, the content of its effective ingredient of Chinese medicine after fermentation can be multiplied.The needs that the applicable large-scale industrial of the present invention produces, have good market prospect.

Description

A kind of preparation method of the Chinese medicine microecological preparation preventing and treating duck pestilence
Technical field
The invention belongs to veterinary drug microbial ecological agent technical field, the preparation method particularly relating to a kind of natural green traditional Chinese medicine for livestock microbial ecological agent preventing and treating duck pestilence.
Background technology
Duck pestilence is commonly called as epidemic infection with swollen head, has another name called duck viral enteritis, and cause of disease is herpetoviridae, is be common in that the one of the geese flying in a line-brothers order birdss such as duck, goose, swan is acute, high degree in contact sexually transmitted disease, one of epidemic disease serious to the harm of world wide aquatic bird aquaculture.Clinical main manifestations is swollen head, shed tears, bipod paralysis, the green loose stool of row, body temperature raise, and esophagus has pseudomembranosa gangrenous inflammation, blood vessel injury, and cloaca is congested, edema and necrosis, and this disease is found in Holland in nineteen twenty-three at first, and China is in nineteen fifty-seven the first explosion.This disease all can occur throughout the year, and popular the most serious at spring and summer and autumn, this disease course of disease is shorter, and sickness rate is high, and aquaculture, up to more than 90%, is threatened serious by mortality rate.This disease of present stage there is no effective Therapeutic Method, and main means of prevention is vaccination and strengthens feeding and management, and treatment means is single and has the hidden danger of immuning failure,
Vaccination is currently mainly adopted to prevent the generation of this disease, basic controlling typicality duck pestilence popular, but the factors such as virus variation, the interference that vaccine uses and improper storage, malnutrition, other diseases antagonist produce all can cause immuning failure, causes that atypical duck pestilence often occurs.If to the amantadine of drug main antiviral of duck pestilence treatment and ribavirin and yolk antibody, zoopery shows that two kinds of medicines all have a teratogenesis, and amantadine also can the generation of inducible resistance strain, prolonged application makes virus develop immunity to drugs to affect the treatment.
Although Chinese veterinary drug preparation SHUANGHUANLIAN, astragalus polysaccharides, QINGKAILING etc. have become as one of main method of veterinary clinic treatment poultry antiviral property disease, the clinical epidemic prevention of epidemic disease plays an important role, but the virosis that many clinics are common is not shown gratifying clinical effectiveness by these medicines, therefore, continue to research and develop effective clinical application and remain one of veterinarian's main task.
And the Chinese medicine microecological preparation of preparation that Chinese medicine is fermented together with microorganism, prior art has no report.
Summary of the invention
It is an object of the invention to provide a kind of preparation method having and preventing and treating duck pestilence disease Chinese medicine microecological preparation, the environmental protection simple, easy to use, safe of this preparation method, pure ecology, pollution-free, noresidue, viable bacteria content are high, and the content of Effective Component of Chinese Medicine can be multiplied.
For reaching above-mentioned purpose, the method that the present invention adopts is as follows:
A kind of preparation method of the Chinese medicine microecological preparation preventing and treating duck pestilence, it is characterized in that: it is prepared by the following method, the intense fire that Flos Lonicerae, Ramulus Cinnamomi, Radix Isatidis, Lysimachia christinae Hance, Indigo Naturalis, Rhizoma Coptidis, the Radix Astragali, Herb Gynostemmae Pentaphylli, Radix Glycyrrhizae added water boils, slow fire insulation 30~60min, cold filtration, collect filtrate, add white sugar to boil, inoculating bacillus subtilis, lactobacillus and Zygosaccharomyces bailii bacterium after cooling down to room temperature through 3~7d fermentation gained microbial ecological agent, gained microbial ecological agent is drunk directly to duck after diluting in proportion.
The preparation method of described a kind of Chinese medicine microecological preparation preventing and treating duck pestilence, it is characterised in that: weigh the Chinese medicine composition of following weight portion: Flos Lonicerae 60~80, Ramulus Cinnamomi 60~80, Radix Isatidis 60~80, Lysimachia christinae Hance 40~60, Indigo Naturalis 40~60, Rhizoma Coptidis 40~60, the Radix Astragali 30~40, Herb Gynostemmae Pentaphylli 30~40, Radix Glycyrrhizae 20~30.
The preparation method of described a kind of Chinese medicine microecological preparation preventing and treating duck pestilence, it is characterised in that: each Chinese herbal medicine is pulverized, weighs the Chinese herbal medicine of weight portion, adding the water of Chinese herbal medicine gross weight 6~8 times amount, soak 30~60min, intense fire boils, slow fire insulation 30~60min, cools down Floor 12 filtered through gauze;Filtering residue adds 4 again~water of 5 times amount, and intense fire boils, slow fire insulation 30~60min, cools down Floor 12 filtered through gauze, merges twice filtrate.
The preparation method of described a kind of Chinese medicine microecological preparation preventing and treating duck pestilence, it is characterized in that: in units of 100Kg filtrate, add white sugar 15~20Kg, and boil 5~10min, it is cooled to room temperature, inoculation bacillus subtilis, lactobacillus and Zygosaccharomyces bailii bacteria preparation 10~15Kg, fermentation is to pH5.5~6.0.
The preparation method of described a kind of Chinese medicine microecological preparation preventing and treating duck pestilence, is characterized in that substituting white sugar with Mel, crystal sugar, glucose, and Mel consumption is 20~25Kg;Crystal sugar consumption is 10~15Kg;Glucose consumption is 21~25Kg.
The preparation method of described a kind of Chinese medicine microecological preparation preventing and treating duck pestilence, it is characterised in that: fermentation is to carry out in aseptic fermenting cellar.
The preparation method of described a kind of Chinese medicine microecological preparation preventing and treating duck pestilence, it is characterised in that: fermentation time is about 3~7 days, and fermentation temperature is 33~37 DEG C.
The preparation method of described a kind of Chinese medicine microecological preparation preventing and treating duck pestilence, it is characterised in that: gained fermentation liquid is drunk directly to duck after diluting 3~8 times:
During prevention duck pestilence, fermentation liquid is diluted 5~8 times, drinks 7 days to duck continuously;
During treatment duck pestilence, fermentation liquid is diluted 3~5 times, drinks 7 days to duck continuously.
Chinese medicine microecological preparation of the present invention have profitable strain quantity in antiviral, inducement interferon, increase intestinal, improve immunologic function, strengthen duck body premunition, the effect such as without any side effects.When the beneficial effects of the present invention is prevention duck pestilence, protective rate reaches more than 90%, and during treatment duck pestilence, cure rate reaches 70%.
Detailed description of the invention
Embodiment 1
The preparation method of a kind of Chinese medicine microecological preparation preventing and treating duck pestilence, comprises the following steps:
Weigh following weight (Kg) Chinese herbal medicine: Flos Lonicerae 60, Ramulus Cinnamomi 60, Radix Isatidis 60, Lysimachia christinae Hance 40, Indigo Naturalis 40, Rhizoma Coptidis 40, the Radix Astragali 30, Herb Gynostemmae Pentaphylli 30, Radix Glycyrrhizae 20.
The preparation of fermentation liquid: add the water of Chinese herbal medicine gross weight 6 times amount, soaks 30min, and intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze;Filtering residue adds the water of 4 times amount again, and intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze, merges twice filtrate.100Kg filtrate is that unit adds white sugar 20Kg, and boils 5min, is cooled to room temperature, and inoculation bacillus subtilis, lactobacillus and Zygosaccharomyces bailii bacteria preparation 5Kg, fermentation is to carry out in aseptic fermenting cellar.Fermentation is to pH5.5.
Gained fermentation liquid is drunk directly to duck after diluting 3~8 times: during prevention duck pestilence, fermentation liquid is diluted 5~8 times, drinks 7 days to duck continuously.
Embodiment 2
The preparation method of a kind of Chinese medicine microecological preparation preventing and treating duck pestilence, comprises the following steps:
Weigh following weight (Kg) Chinese herbal medicine: Flos Lonicerae 80, Ramulus Cinnamomi 80, Radix Isatidis 80, Lysimachia christinae Hance 60, Indigo Naturalis 60, Rhizoma Coptidis 60, the Radix Astragali 40, Herb Gynostemmae Pentaphylli 40, Radix Glycyrrhizae 30.The preparation of fermentation liquid: add the water of Chinese herbal medicine gross weight 8 times amount, soaks 60min, and intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze;Filtering residue adds the water of 5 times amount again, and intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze, merges twice filtrate.100Kg filtrate is that unit adds white sugar 25Kg, and boils 5~10min, is cooled to room temperature, and inoculation bacillus subtilis, lactobacillus and Zygosaccharomyces bailii bacteria preparation 10Kg, fermentation is to carry out in aseptic fermenting cellar.Fermentation is to pH6.0.
Gained fermentation liquid is drunk directly to duck after diluting 3~8 times: during treatment duck pestilence, fermentation liquid is diluted 3~5 times, drinks 7 days to duck continuously.
Embodiment 3
Clinical trial example: on May 15th, 2013, the meat-type duck morbidity of 5000 35 ages in days that North Huaihe River Area of Anhui Province's ducks specialized household is raised, clinical symptoms: blepharoedema, shed tears, bipod paralysis, the green loose stool of row, body temperature raise, lethargy, bowing necking down, appetite reduces, and cuing open the visible esophagus of inspection has pseudomembranosa gangrenous inflammation, blood vessel injury, serous coat and internal organs have hemorrhage shape speckle, and clinical diagnosis is duck pestilence.Morbidity duck number 957, carries out isolated rearing to morbidity duck, then dilutes 3~5 times with the microbial ecological agent described in embodiment 2, drinks 7 days to duck continuously, after medication 2 hours, the sick duck mental status is clearly better, and stops death after 3 days, dead 288 altogether, all the other sick ducks are almost recovered, cure rate 69.90%.To the duck not showing clinical symptoms, then dilute 5~8 times with the microbial ecological agent described in embodiment 1, drink 7 days to duck continuously, the duck not fallen ill in feeding process still later occurs 393 atypical duck pestilence symptoms, protective rate 90.27%.

Claims (5)

1. the preparation method of the Chinese medicine microecological preparation preventing and treating duck pestilence, it is characterized in that: it is to make by the Chinese medicine of following weight portion, Flos Lonicerae 60~80, Ramulus Cinnamomi 60~80, Radix Isatidis 60~80, Lysimachia christinae Hance 40~60, Indigo Naturalis 40~60, Rhizoma Coptidis 40~60, the Radix Astragali 30~40, Herb Gynostemmae Pentaphylli 30~40, Radix Glycyrrhizae 20~30;Being pulverized by each Chinese medicine, weigh the Chinese medicine of above-mentioned weight portion, add the water of Chinese herbal medicine gross weight 6~8 times amount, soak 30~60min, intense fire boils, slow fire insulation 30~60min, cools down Floor 12 filtered through gauze;Filtering residue adds 4 again~water of 5 times amount, and intense fire boils, slow fire insulation 30~60min, cools down Floor 12 filtered through gauze, merges twice filtrate, adds white sugar and boil;Bacillus subtilis, lactobacillus and Zygosaccharomyces bailii bacterium is inoculated through 3~7d fermentation gained microbial ecological agent after cooling down to room temperature;Gained microbial ecological agent is drunk directly to duck after diluting in proportion.
2. the preparation method of a kind of Chinese medicine microecological preparation preventing and treating duck pestilence according to claim 1, it is characterized in that: in units of 100Kg filtrate, add white sugar 15~20Kg, and boil 5~10min, it is cooled to room temperature, inoculation bacillus subtilis, lactobacillus and Zygosaccharomyces bailii bacteria preparation 10~15Kg, fermentation is to pH5.5~6.0.
3. the preparation method of a kind of Chinese medicine microecological preparation preventing and treating duck pestilence according to claim 1, it is characterised in that: fermentation is to carry out in aseptic fermenting cellar.
4. the preparation method of a kind of Chinese medicine microecological preparation preventing and treating duck pestilence according to claim 1, it is characterised in that: fermentation time is 3~7 days, and fermentation temperature is 33~37 DEG C.
5. the preparation method of a kind of Chinese medicine microecological preparation preventing and treating duck pestilence according to claim 1, it is characterised in that: during prevention duck pestilence, fermentation liquid is diluted 5~8 times, drinks 7 days to duck continuously;During treatment duck pestilence, fermentation liquid is diluted 3~5 times, drinks 7 days to duck continuously.
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CN110463857A (en) * 2019-09-17 2019-11-19 中国农业科学院哈尔滨兽医研究所 A kind of preparation method of feed that preventing and treating duck plague

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