CN104093857A - 核酸的定量、高度多重检测 - Google Patents

核酸的定量、高度多重检测 Download PDF

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Publication number
CN104093857A
CN104093857A CN201280067273.7A CN201280067273A CN104093857A CN 104093857 A CN104093857 A CN 104093857A CN 201280067273 A CN201280067273 A CN 201280067273A CN 104093857 A CN104093857 A CN 104093857A
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CN
China
Prior art keywords
probe
array
nucleic acid
capture
signal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201280067273.7A
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English (en)
Chinese (zh)
Inventor
K·斯卡布
P·马丁
B·塔弗特
J·拉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nvs Technical Concern Co Ltd
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Nvs Technical Concern Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/399,872 external-priority patent/US20120214686A1/en
Application filed by Nvs Technical Concern Co Ltd filed Critical Nvs Technical Concern Co Ltd
Publication of CN104093857A publication Critical patent/CN104093857A/zh
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201280067273.7A 2011-11-17 2012-08-16 核酸的定量、高度多重检测 Pending CN104093857A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201161561198P 2011-11-17 2011-11-17
US61/561,198 2011-11-17
USPCT/US2012/025699 2012-02-17
US13/399,872 US20120214686A1 (en) 2011-02-18 2012-02-17 Quantitative, Highly Multiplexed Detection of Nucleic Acids
PCT/US2012/025699 WO2012112925A2 (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids
US13/399,872 2012-02-17
PCT/US2012/051236 WO2013074163A1 (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids

Publications (1)

Publication Number Publication Date
CN104093857A true CN104093857A (zh) 2014-10-08

Family

ID=48430026

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280067273.7A Pending CN104093857A (zh) 2011-11-17 2012-08-16 核酸的定量、高度多重检测

Country Status (12)

Country Link
EP (1) EP2780479A4 (enExample)
JP (1) JP2014533502A (enExample)
KR (1) KR20140094007A (enExample)
CN (1) CN104093857A (enExample)
AU (1) AU2012337389A1 (enExample)
BR (1) BR112014011937A2 (enExample)
CA (1) CA2855953A1 (enExample)
HK (1) HK1199746A1 (enExample)
IL (1) IL232586A0 (enExample)
MX (1) MX338076B (enExample)
SG (1) SG11201402341QA (enExample)
WO (1) WO2013074163A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107735403A (zh) * 2015-04-16 2018-02-23 威廉马歇莱思大学 由辅助寡核苷酸物质对核酸杂交探针的化学计量调节
CN110016500A (zh) * 2018-01-10 2019-07-16 深圳闪量科技有限公司 表面探针定量pcr方法
CN111808989A (zh) * 2020-06-18 2020-10-23 重庆浦洛通基因医学研究院有限公司 一种冠状病毒/流感病毒/鼻病毒核酸联合检测试剂盒及使用方法
CN115210760A (zh) * 2019-11-21 2022-10-18 10X基因组学有限公司 分析物的空间分析

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015008985A1 (en) * 2013-07-15 2015-01-22 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
EP4442838A3 (en) * 2020-10-15 2024-12-25 Genentech, Inc. Multiplexed target-binding candidate screening analysis

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005010199A2 (en) * 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout
WO2005068660A1 (en) * 2003-12-19 2005-07-28 Beckman Coulter, Inc. Solid-phase multiplexed invader assay
US20080241838A1 (en) * 2006-12-29 2008-10-02 Applera Corporation, Applied Biosystems Group Methods and systems for detecting nucleic acids

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5422253A (en) * 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
AU2002363628A1 (en) * 2001-07-17 2003-05-26 Stratagene Methods for detection of a target nucleic acid by capture using multi-subunit probes
CA2672246A1 (en) * 2006-06-30 2008-03-13 Rosetta Genomics Ltd Detecting nucleic acids
EP2077336A1 (en) * 2007-12-19 2009-07-08 Koninklijke Philips Electronics N.V. Device and method for parallel quantitative analysis of multiple nucleic acids
CN102224257A (zh) * 2008-11-21 2011-10-19 皇家飞利浦电子股份有限公司 使用双链核酸特异性染料在固体表面上的实时多重pcr检测

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005010199A2 (en) * 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout
WO2005068660A1 (en) * 2003-12-19 2005-07-28 Beckman Coulter, Inc. Solid-phase multiplexed invader assay
US20080241838A1 (en) * 2006-12-29 2008-10-02 Applera Corporation, Applied Biosystems Group Methods and systems for detecting nucleic acids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BERNARD JUSKOWIAK: "Nucleic acid-based fluorescent probes and their analytical potential", 《ANAL BIOANAL CHEM》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107735403A (zh) * 2015-04-16 2018-02-23 威廉马歇莱思大学 由辅助寡核苷酸物质对核酸杂交探针的化学计量调节
CN107735403B (zh) * 2015-04-16 2021-06-11 威廉马歇莱思大学 由辅助寡核苷酸物质对核酸杂交探针的化学计量调节
US11879152B2 (en) 2015-04-16 2024-01-23 William Marsh Rice University Stoichiometric tuning of nucleic acid hybridization probes by auxiliary oligonucleotide species
CN110016500A (zh) * 2018-01-10 2019-07-16 深圳闪量科技有限公司 表面探针定量pcr方法
CN115210760A (zh) * 2019-11-21 2022-10-18 10X基因组学有限公司 分析物的空间分析
CN115210760B (zh) * 2019-11-21 2023-08-01 10X基因组学有限公司 分析物的空间分析
CN111808989A (zh) * 2020-06-18 2020-10-23 重庆浦洛通基因医学研究院有限公司 一种冠状病毒/流感病毒/鼻病毒核酸联合检测试剂盒及使用方法

Also Published As

Publication number Publication date
SG11201402341QA (en) 2014-06-27
KR20140094007A (ko) 2014-07-29
WO2013074163A1 (en) 2013-05-23
HK1199746A1 (en) 2015-07-17
BR112014011937A2 (pt) 2017-05-30
JP2014533502A (ja) 2014-12-15
EP2780479A4 (en) 2015-04-08
IL232586A0 (en) 2014-06-30
EP2780479A1 (en) 2014-09-24
MX338076B (es) 2016-04-01
AU2012337389A1 (en) 2014-06-19
MX2014005912A (es) 2014-09-04
CA2855953A1 (en) 2013-05-23

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Application publication date: 20141008