CN104093857A - 核酸的定量、高度多重检测 - Google Patents

核酸的定量、高度多重检测 Download PDF

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Publication number
CN104093857A
CN104093857A CN201280067273.7A CN201280067273A CN104093857A CN 104093857 A CN104093857 A CN 104093857A CN 201280067273 A CN201280067273 A CN 201280067273A CN 104093857 A CN104093857 A CN 104093857A
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CN
China
Prior art keywords
probe
array
nucleic acid
capture
signal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201280067273.7A
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English (en)
Chinese (zh)
Inventor
K·斯卡布
P·马丁
B·塔弗特
J·拉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nvs Technical Concern Co Ltd
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Nvs Technical Concern Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2012/025699 external-priority patent/WO2012112925A2/en
Application filed by Nvs Technical Concern Co Ltd filed Critical Nvs Technical Concern Co Ltd
Publication of CN104093857A publication Critical patent/CN104093857A/zh
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201280067273.7A 2011-11-17 2012-08-16 核酸的定量、高度多重检测 Pending CN104093857A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201161561198P 2011-11-17 2011-11-17
US61/561,198 2011-11-17
PCT/US2012/025699 WO2012112925A2 (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids
US13/399,872 2012-02-17
USPCT/US2012/025699 2012-02-17
US13/399,872 US20120214686A1 (en) 2011-02-18 2012-02-17 Quantitative, Highly Multiplexed Detection of Nucleic Acids
PCT/US2012/051236 WO2013074163A1 (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids

Publications (1)

Publication Number Publication Date
CN104093857A true CN104093857A (zh) 2014-10-08

Family

ID=48430026

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280067273.7A Pending CN104093857A (zh) 2011-11-17 2012-08-16 核酸的定量、高度多重检测

Country Status (12)

Country Link
EP (1) EP2780479A4 (enrdf_load_stackoverflow)
JP (1) JP2014533502A (enrdf_load_stackoverflow)
KR (1) KR20140094007A (enrdf_load_stackoverflow)
CN (1) CN104093857A (enrdf_load_stackoverflow)
AU (1) AU2012337389A1 (enrdf_load_stackoverflow)
BR (1) BR112014011937A2 (enrdf_load_stackoverflow)
CA (1) CA2855953A1 (enrdf_load_stackoverflow)
HK (1) HK1199746A1 (enrdf_load_stackoverflow)
IL (1) IL232586A0 (enrdf_load_stackoverflow)
MX (1) MX338076B (enrdf_load_stackoverflow)
SG (1) SG11201402341QA (enrdf_load_stackoverflow)
WO (1) WO2013074163A1 (enrdf_load_stackoverflow)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107735403A (zh) * 2015-04-16 2018-02-23 威廉马歇莱思大学 由辅助寡核苷酸物质对核酸杂交探针的化学计量调节
CN110016500A (zh) * 2018-01-10 2019-07-16 深圳闪量科技有限公司 表面探针定量pcr方法
CN111808989A (zh) * 2020-06-18 2020-10-23 重庆浦洛通基因医学研究院有限公司 一种冠状病毒/流感病毒/鼻病毒核酸联合检测试剂盒及使用方法
CN115210760A (zh) * 2019-11-21 2022-10-18 10X基因组学有限公司 分析物的空间分析

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015008985A1 (en) * 2013-07-15 2015-01-22 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
CN116438318A (zh) * 2020-10-15 2023-07-14 基因泰克公司 多重靶标结合候选筛选分析

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005010199A2 (en) * 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout
WO2005068660A1 (en) * 2003-12-19 2005-07-28 Beckman Coulter, Inc. Solid-phase multiplexed invader assay
US20080241838A1 (en) * 2006-12-29 2008-10-02 Applera Corporation, Applied Biosystems Group Methods and systems for detecting nucleic acids

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Publication number Priority date Publication date Assignee Title
US5422253A (en) * 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
WO2003042353A2 (en) * 2001-07-17 2003-05-22 Stratagene Methods for detection of a target nucleic acid by capture using multi-subunit probes
US9115389B2 (en) * 2006-06-30 2015-08-25 Rosetta Genomics Ltd. Method for detecting a target nucleic acid comprising two portions using probes having a first portion complementary to the first portion of the target nucleic acid and a second portion substantially complementary to the second portion of the target nucleic acid
EP2077336A1 (en) * 2007-12-19 2009-07-08 Koninklijke Philips Electronics N.V. Device and method for parallel quantitative analysis of multiple nucleic acids
US20120040853A1 (en) * 2008-11-21 2012-02-16 Koninklijke Philips Electronics N.V. Real time multiplex pcr detection on solid surfaces using double stranded nucleic acid specific dyes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005010199A2 (en) * 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout
WO2005068660A1 (en) * 2003-12-19 2005-07-28 Beckman Coulter, Inc. Solid-phase multiplexed invader assay
US20080241838A1 (en) * 2006-12-29 2008-10-02 Applera Corporation, Applied Biosystems Group Methods and systems for detecting nucleic acids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BERNARD JUSKOWIAK: "Nucleic acid-based fluorescent probes and their analytical potential", 《ANAL BIOANAL CHEM》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107735403A (zh) * 2015-04-16 2018-02-23 威廉马歇莱思大学 由辅助寡核苷酸物质对核酸杂交探针的化学计量调节
CN107735403B (zh) * 2015-04-16 2021-06-11 威廉马歇莱思大学 由辅助寡核苷酸物质对核酸杂交探针的化学计量调节
US11879152B2 (en) 2015-04-16 2024-01-23 William Marsh Rice University Stoichiometric tuning of nucleic acid hybridization probes by auxiliary oligonucleotide species
CN110016500A (zh) * 2018-01-10 2019-07-16 深圳闪量科技有限公司 表面探针定量pcr方法
CN115210760A (zh) * 2019-11-21 2022-10-18 10X基因组学有限公司 分析物的空间分析
CN115210760B (zh) * 2019-11-21 2023-08-01 10X基因组学有限公司 分析物的空间分析
CN111808989A (zh) * 2020-06-18 2020-10-23 重庆浦洛通基因医学研究院有限公司 一种冠状病毒/流感病毒/鼻病毒核酸联合检测试剂盒及使用方法

Also Published As

Publication number Publication date
BR112014011937A2 (pt) 2017-05-30
SG11201402341QA (en) 2014-06-27
WO2013074163A1 (en) 2013-05-23
CA2855953A1 (en) 2013-05-23
MX2014005912A (es) 2014-09-04
JP2014533502A (ja) 2014-12-15
KR20140094007A (ko) 2014-07-29
EP2780479A4 (en) 2015-04-08
MX338076B (es) 2016-04-01
EP2780479A1 (en) 2014-09-24
AU2012337389A1 (en) 2014-06-19
HK1199746A1 (en) 2015-07-17
IL232586A0 (en) 2014-06-30

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Application publication date: 20141008