CN104093857A - 核酸的定量、高度多重检测 - Google Patents
核酸的定量、高度多重检测 Download PDFInfo
- Publication number
- CN104093857A CN104093857A CN201280067273.7A CN201280067273A CN104093857A CN 104093857 A CN104093857 A CN 104093857A CN 201280067273 A CN201280067273 A CN 201280067273A CN 104093857 A CN104093857 A CN 104093857A
- Authority
- CN
- China
- Prior art keywords
- probe
- array
- nucleic acid
- capture
- signal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 159
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 144
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 144
- 238000001514 detection method Methods 0.000 title claims description 87
- 238000000034 method Methods 0.000 claims abstract description 157
- 238000006243 chemical reaction Methods 0.000 claims abstract description 91
- 239000000523 sample Substances 0.000 claims description 415
- 239000000463 material Substances 0.000 claims description 106
- 239000012634 fragment Substances 0.000 claims description 97
- 230000003321 amplification Effects 0.000 claims description 78
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 78
- 238000009396 hybridization Methods 0.000 claims description 68
- 239000003550 marker Substances 0.000 claims description 44
- 239000003153 chemical reaction reagent Substances 0.000 claims description 16
- 230000008878 coupling Effects 0.000 claims description 13
- 238000010168 coupling process Methods 0.000 claims description 13
- 238000005859 coupling reaction Methods 0.000 claims description 13
- 238000005259 measurement Methods 0.000 claims description 12
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 11
- 239000002773 nucleotide Substances 0.000 claims description 10
- 125000003729 nucleotide group Chemical group 0.000 claims description 10
- 101710163270 Nuclease Proteins 0.000 claims description 9
- 230000000670 limiting effect Effects 0.000 claims description 6
- 238000003705 background correction Methods 0.000 claims description 4
- 238000002955 isolation Methods 0.000 claims 1
- 238000003491 array Methods 0.000 abstract description 7
- 101150104383 ALOX5AP gene Proteins 0.000 description 49
- 101100236114 Mus musculus Lrrfip1 gene Proteins 0.000 description 49
- 230000003287 optical effect Effects 0.000 description 41
- 238000012360 testing method Methods 0.000 description 34
- 238000005516 engineering process Methods 0.000 description 28
- 238000013461 design Methods 0.000 description 26
- 239000012530 fluid Substances 0.000 description 26
- 108091093088 Amplicon Proteins 0.000 description 23
- 238000000576 coating method Methods 0.000 description 20
- -1 for example pottery Substances 0.000 description 20
- 238000004519 manufacturing process Methods 0.000 description 19
- 239000011248 coating agent Substances 0.000 description 18
- 238000004458 analytical method Methods 0.000 description 17
- 238000005520 cutting process Methods 0.000 description 17
- 108020004414 DNA Proteins 0.000 description 16
- 239000012071 phase Substances 0.000 description 16
- 230000008569 process Effects 0.000 description 16
- 230000000295 complement effect Effects 0.000 description 13
- 238000005382 thermal cycling Methods 0.000 description 13
- 239000011521 glass Substances 0.000 description 12
- 230000003750 conditioning effect Effects 0.000 description 11
- 238000003753 real-time PCR Methods 0.000 description 11
- 238000001228 spectrum Methods 0.000 description 11
- 239000000758 substrate Substances 0.000 description 11
- 239000003795 chemical substances by application Substances 0.000 description 10
- 238000013016 damping Methods 0.000 description 10
- 238000002493 microarray Methods 0.000 description 10
- 239000000203 mixture Substances 0.000 description 10
- 239000004820 Pressure-sensitive adhesive Substances 0.000 description 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 9
- 238000012545 processing Methods 0.000 description 9
- 239000000975 dye Substances 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 238000003499 nucleic acid array Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 230000008859 change Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 150000002148 esters Chemical class 0.000 description 7
- 230000005284 excitation Effects 0.000 description 7
- 239000004417 polycarbonate Substances 0.000 description 7
- 230000002829 reductive effect Effects 0.000 description 7
- 238000000018 DNA microarray Methods 0.000 description 6
- 238000012408 PCR amplification Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000010410 layer Substances 0.000 description 6
- 230000000149 penetrating effect Effects 0.000 description 6
- 229920000642 polymer Chemical group 0.000 description 6
- 235000012239 silicon dioxide Nutrition 0.000 description 6
- 230000000007 visual effect Effects 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 240000002853 Nelumbo nucifera Species 0.000 description 5
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 5
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 5
- 239000002253 acid Substances 0.000 description 5
- 238000007834 ligase chain reaction Methods 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- 230000005855 radiation Effects 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- 238000004448 titration Methods 0.000 description 5
- 238000003466 welding Methods 0.000 description 5
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 238000000695 excitation spectrum Methods 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 238000010791 quenching Methods 0.000 description 4
- 239000011541 reaction mixture Substances 0.000 description 4
- 229920006395 saturated elastomer Polymers 0.000 description 4
- 230000011664 signaling Effects 0.000 description 4
- 239000007790 solid phase Substances 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- NOWKCMXCCJGMRR-UHFFFAOYSA-N Aziridine Chemical compound C1CN1 NOWKCMXCCJGMRR-UHFFFAOYSA-N 0.000 description 3
- 239000004593 Epoxy Substances 0.000 description 3
- PEEHTFAAVSWFBL-UHFFFAOYSA-N Maleimide Chemical compound O=C1NC(=O)C=C1 PEEHTFAAVSWFBL-UHFFFAOYSA-N 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 241000489455 Sitta europaea Species 0.000 description 3
- 150000001266 acyl halides Chemical class 0.000 description 3
- 150000001299 aldehydes Chemical class 0.000 description 3
- 150000001336 alkenes Chemical class 0.000 description 3
- 150000001345 alkine derivatives Chemical class 0.000 description 3
- 150000001408 amides Chemical class 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 230000000712 assembly Effects 0.000 description 3
- 238000000429 assembly Methods 0.000 description 3
- 150000001540 azides Chemical class 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000001351 cycling effect Effects 0.000 description 3
- 238000006352 cycloaddition reaction Methods 0.000 description 3
- 150000001993 dienes Chemical class 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 238000007599 discharging Methods 0.000 description 3
- CCGKOQOJPYTBIH-UHFFFAOYSA-N ethenone Chemical compound C=C=O CCGKOQOJPYTBIH-UHFFFAOYSA-N 0.000 description 3
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 150000002576 ketones Chemical class 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 230000001915 proofreading effect Effects 0.000 description 3
- 239000002096 quantum dot Substances 0.000 description 3
- 239000010453 quartz Substances 0.000 description 3
- 238000011897 real-time detection Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000000377 silicon dioxide Substances 0.000 description 3
- 229960001866 silicon dioxide Drugs 0.000 description 3
- 238000004088 simulation Methods 0.000 description 3
- 230000003595 spectral effect Effects 0.000 description 3
- 150000007970 thio esters Chemical class 0.000 description 3
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- 241000711573 Coronaviridae Species 0.000 description 2
- 102000004533 Endonucleases Human genes 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 2
- 101100444396 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ECM30 gene Proteins 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 239000002872 contrast media Substances 0.000 description 2
- 238000007405 data analysis Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 230000008034 disappearance Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000000835 electrochemical detection Methods 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 238000009432 framing Methods 0.000 description 2
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000012775 microarray technology Methods 0.000 description 2
- 239000003068 molecular probe Substances 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 238000001216 nucleic acid method Methods 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 238000006384 oligomerization reaction Methods 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 238000005375 photometry Methods 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000011895 specific detection Methods 0.000 description 2
- 239000002344 surface layer Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000010189 synthetic method Methods 0.000 description 2
- 230000005619 thermoelectricity Effects 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- UDGUGZTYGWUUSG-UHFFFAOYSA-N 4-[4-[[2,5-dimethoxy-4-[(4-nitrophenyl)diazenyl]phenyl]diazenyl]-n-methylanilino]butanoic acid Chemical compound COC=1C=C(N=NC=2C=CC(=CC=2)N(C)CCCC(O)=O)C(OC)=CC=1N=NC1=CC=C([N+]([O-])=O)C=C1 UDGUGZTYGWUUSG-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 240000000662 Anethum graveolens Species 0.000 description 1
- 208000019901 Anxiety disease Diseases 0.000 description 1
- 102000007527 Autoreceptors Human genes 0.000 description 1
- 108010071131 Autoreceptors Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 238000012351 Integrated analysis Methods 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 101001031591 Mus musculus Heart- and neural crest derivatives-expressed protein 2 Proteins 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- 241001040659 Plasmodium (Plasmodium) Species 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000002146 bilateral effect Effects 0.000 description 1
- 238000011953 bioanalysis Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000004163 cytometry Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000010891 electric arc Methods 0.000 description 1
- 238000010894 electron beam technology Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 239000003822 epoxy resin Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000010954 inorganic particle Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 238000003913 materials processing Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001921 nucleic acid quantification Methods 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 230000005693 optoelectronics Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000011049 pearl Substances 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
- 239000013615 primer Substances 0.000 description 1
- 239000002987 primer (paints) Substances 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 125000006239 protecting group Chemical group 0.000 description 1
- NHDHVHZZCFYRSB-UHFFFAOYSA-N pyriproxyfen Chemical compound C=1C=CC=NC=1OC(C)COC(C=C1)=CC=C1OC1=CC=CC=C1 NHDHVHZZCFYRSB-UHFFFAOYSA-N 0.000 description 1
- 230000005616 pyroelectricity Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000010223 real-time analysis Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 238000002076 thermal analysis method Methods 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000013024 troubleshooting Methods 0.000 description 1
- 238000002371 ultraviolet--visible spectrum Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161561198P | 2011-11-17 | 2011-11-17 | |
US61/561,198 | 2011-11-17 | ||
PCT/US2012/025699 WO2012112925A2 (en) | 2011-02-18 | 2012-02-17 | Quantitative, highly multiplexed detection of nucleic acids |
US13/399,872 | 2012-02-17 | ||
USPCT/US2012/025699 | 2012-02-17 | ||
US13/399,872 US20120214686A1 (en) | 2011-02-18 | 2012-02-17 | Quantitative, Highly Multiplexed Detection of Nucleic Acids |
PCT/US2012/051236 WO2013074163A1 (en) | 2011-11-17 | 2012-08-16 | Quantitative, highly multiplexed detection of nucleic acids |
Publications (1)
Publication Number | Publication Date |
---|---|
CN104093857A true CN104093857A (zh) | 2014-10-08 |
Family
ID=48430026
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201280067273.7A Pending CN104093857A (zh) | 2011-11-17 | 2012-08-16 | 核酸的定量、高度多重检测 |
Country Status (12)
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107735403A (zh) * | 2015-04-16 | 2018-02-23 | 威廉马歇莱思大学 | 由辅助寡核苷酸物质对核酸杂交探针的化学计量调节 |
CN110016500A (zh) * | 2018-01-10 | 2019-07-16 | 深圳闪量科技有限公司 | 表面探针定量pcr方法 |
CN111808989A (zh) * | 2020-06-18 | 2020-10-23 | 重庆浦洛通基因医学研究院有限公司 | 一种冠状病毒/流感病毒/鼻病毒核酸联合检测试剂盒及使用方法 |
CN115210760A (zh) * | 2019-11-21 | 2022-10-18 | 10X基因组学有限公司 | 分析物的空间分析 |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015008985A1 (en) * | 2013-07-15 | 2015-01-22 | Seegene, Inc. | Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization |
CN116438318A (zh) * | 2020-10-15 | 2023-07-14 | 基因泰克公司 | 多重靶标结合候选筛选分析 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005010199A2 (en) * | 2003-07-16 | 2005-02-03 | Geneohm Sciences, Inc. | Invasive cleavage reaction with electrochemical readout |
WO2005068660A1 (en) * | 2003-12-19 | 2005-07-28 | Beckman Coulter, Inc. | Solid-phase multiplexed invader assay |
US20080241838A1 (en) * | 2006-12-29 | 2008-10-02 | Applera Corporation, Applied Biosystems Group | Methods and systems for detecting nucleic acids |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5422253A (en) * | 1992-12-07 | 1995-06-06 | Wisconsin Alumni Research Foundation | Method of site specific nucleic acid cleavage |
US6350580B1 (en) * | 2000-10-11 | 2002-02-26 | Stratagene | Methods for detection of a target nucleic acid using a probe comprising secondary structure |
WO2003042353A2 (en) * | 2001-07-17 | 2003-05-22 | Stratagene | Methods for detection of a target nucleic acid by capture using multi-subunit probes |
US9115389B2 (en) * | 2006-06-30 | 2015-08-25 | Rosetta Genomics Ltd. | Method for detecting a target nucleic acid comprising two portions using probes having a first portion complementary to the first portion of the target nucleic acid and a second portion substantially complementary to the second portion of the target nucleic acid |
EP2077336A1 (en) * | 2007-12-19 | 2009-07-08 | Koninklijke Philips Electronics N.V. | Device and method for parallel quantitative analysis of multiple nucleic acids |
US20120040853A1 (en) * | 2008-11-21 | 2012-02-16 | Koninklijke Philips Electronics N.V. | Real time multiplex pcr detection on solid surfaces using double stranded nucleic acid specific dyes |
-
2012
- 2012-08-16 EP EP12849697.3A patent/EP2780479A4/en not_active Withdrawn
- 2012-08-16 WO PCT/US2012/051236 patent/WO2013074163A1/en active Application Filing
- 2012-08-16 CN CN201280067273.7A patent/CN104093857A/zh active Pending
- 2012-08-16 BR BR112014011937A patent/BR112014011937A2/pt not_active IP Right Cessation
- 2012-08-16 CA CA2855953A patent/CA2855953A1/en not_active Abandoned
- 2012-08-16 JP JP2014542300A patent/JP2014533502A/ja active Pending
- 2012-08-16 AU AU2012337389A patent/AU2012337389A1/en not_active Abandoned
- 2012-08-16 HK HK15100214.3A patent/HK1199746A1/xx unknown
- 2012-08-16 SG SG11201402341QA patent/SG11201402341QA/en unknown
- 2012-08-16 MX MX2014005912A patent/MX338076B/es active IP Right Grant
- 2012-08-16 KR KR1020147016411A patent/KR20140094007A/ko not_active Withdrawn
-
2014
- 2014-05-13 IL IL232586A patent/IL232586A0/en unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005010199A2 (en) * | 2003-07-16 | 2005-02-03 | Geneohm Sciences, Inc. | Invasive cleavage reaction with electrochemical readout |
WO2005068660A1 (en) * | 2003-12-19 | 2005-07-28 | Beckman Coulter, Inc. | Solid-phase multiplexed invader assay |
US20080241838A1 (en) * | 2006-12-29 | 2008-10-02 | Applera Corporation, Applied Biosystems Group | Methods and systems for detecting nucleic acids |
Non-Patent Citations (1)
Title |
---|
BERNARD JUSKOWIAK: "Nucleic acid-based fluorescent probes and their analytical potential", 《ANAL BIOANAL CHEM》 * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107735403A (zh) * | 2015-04-16 | 2018-02-23 | 威廉马歇莱思大学 | 由辅助寡核苷酸物质对核酸杂交探针的化学计量调节 |
CN107735403B (zh) * | 2015-04-16 | 2021-06-11 | 威廉马歇莱思大学 | 由辅助寡核苷酸物质对核酸杂交探针的化学计量调节 |
US11879152B2 (en) | 2015-04-16 | 2024-01-23 | William Marsh Rice University | Stoichiometric tuning of nucleic acid hybridization probes by auxiliary oligonucleotide species |
CN110016500A (zh) * | 2018-01-10 | 2019-07-16 | 深圳闪量科技有限公司 | 表面探针定量pcr方法 |
CN115210760A (zh) * | 2019-11-21 | 2022-10-18 | 10X基因组学有限公司 | 分析物的空间分析 |
CN115210760B (zh) * | 2019-11-21 | 2023-08-01 | 10X基因组学有限公司 | 分析物的空间分析 |
CN111808989A (zh) * | 2020-06-18 | 2020-10-23 | 重庆浦洛通基因医学研究院有限公司 | 一种冠状病毒/流感病毒/鼻病毒核酸联合检测试剂盒及使用方法 |
Also Published As
Publication number | Publication date |
---|---|
BR112014011937A2 (pt) | 2017-05-30 |
SG11201402341QA (en) | 2014-06-27 |
WO2013074163A1 (en) | 2013-05-23 |
CA2855953A1 (en) | 2013-05-23 |
MX2014005912A (es) | 2014-09-04 |
JP2014533502A (ja) | 2014-12-15 |
KR20140094007A (ko) | 2014-07-29 |
EP2780479A4 (en) | 2015-04-08 |
MX338076B (es) | 2016-04-01 |
EP2780479A1 (en) | 2014-09-24 |
AU2012337389A1 (en) | 2014-06-19 |
HK1199746A1 (en) | 2015-07-17 |
IL232586A0 (en) | 2014-06-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103502471B (zh) | 核酸的定量、高度多重检测 | |
CN105102630A (zh) | 试验方法和系统 | |
Hu et al. | Advances in single quantum dot-based nanosensors | |
CN104093857A (zh) | 核酸的定量、高度多重检测 | |
US20110281740A1 (en) | Methods for Real Time Single Molecule Sequencing | |
EA007652B1 (ru) | Способ обнаружения и анализа полинуклеотидов с помощью светособирающих полихромофоров | |
CN101981204A (zh) | 固体支持物上的靶分子的检测和/或定量 | |
JP2013539654A (ja) | フローチップアッセイにおける核酸標的のリアルタイム増幅およびマイクロアレイに基づく検出 | |
CN102428189A (zh) | 反应筒内多重核酸序列的检测 | |
CN115515626A (zh) | 用于检测冠状病毒的组合物和方法 | |
Magiati et al. | A fluorometric lateral flow assay for visual detection of nucleic acids using a digital camera readout | |
CN115803111A (zh) | 用于检测核酸的装置、测定和方法 | |
US20190178804A1 (en) | Light-absorbing optical fiber-based systems and methods | |
US8703653B2 (en) | Quantitative, highly multiplexed detection of nucleic acids | |
JP2004187581A (ja) | 標的核酸の多型決定方法 | |
Naorungroj et al. | Fluorescence origami paper–based analytical device based on strand displacement assay for SARS-CoV-2 cDNA detection | |
WO2024235904A1 (en) | Detection of target analytes using multimodal signal probes | |
Emory | Single-Molecule Detection of Unique Genome Signatures: Applications in Molecular Diagnostics and Homeland Security | |
Piunno et al. | Toward the development of optical nucleic acid biosensors based on TIRF and TCSPC for high sensitivity determinations | |
Máčala | Nanoparticles for Optical Biosensors | |
CN106687599A (zh) | 不存在小沟结合物的直接定量pcr |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WD01 | Invention patent application deemed withdrawn after publication | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141008 |