EP2780479A4 - QUANTITATIVE HIGHLY MULTIPLEXED DETECTION OF NUCLEIC ACIDS - Google Patents

QUANTITATIVE HIGHLY MULTIPLEXED DETECTION OF NUCLEIC ACIDS

Info

Publication number
EP2780479A4
EP2780479A4 EP12849697.3A EP12849697A EP2780479A4 EP 2780479 A4 EP2780479 A4 EP 2780479A4 EP 12849697 A EP12849697 A EP 12849697A EP 2780479 A4 EP2780479 A4 EP 2780479A4
Authority
EP
European Patent Office
Prior art keywords
quantitative
nucleic acids
highly multiplexed
multiplexed detection
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12849697.3A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP2780479A1 (en
Inventor
Kris Scaboo
Patrick Martin
Brad Taft
Jason La
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NVS TECHNOLOGIES Inc
Original Assignee
NVS TECHNOLOGIES Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2012/025699 external-priority patent/WO2012112925A2/en
Application filed by NVS TECHNOLOGIES Inc filed Critical NVS TECHNOLOGIES Inc
Publication of EP2780479A1 publication Critical patent/EP2780479A1/en
Publication of EP2780479A4 publication Critical patent/EP2780479A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP12849697.3A 2011-11-17 2012-08-16 QUANTITATIVE HIGHLY MULTIPLEXED DETECTION OF NUCLEIC ACIDS Withdrawn EP2780479A4 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161561198P 2011-11-17 2011-11-17
PCT/US2012/025699 WO2012112925A2 (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids
US13/399,872 US20120214686A1 (en) 2011-02-18 2012-02-17 Quantitative, Highly Multiplexed Detection of Nucleic Acids
PCT/US2012/051236 WO2013074163A1 (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids

Publications (2)

Publication Number Publication Date
EP2780479A1 EP2780479A1 (en) 2014-09-24
EP2780479A4 true EP2780479A4 (en) 2015-04-08

Family

ID=48430026

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12849697.3A Withdrawn EP2780479A4 (en) 2011-11-17 2012-08-16 QUANTITATIVE HIGHLY MULTIPLEXED DETECTION OF NUCLEIC ACIDS

Country Status (12)

Country Link
EP (1) EP2780479A4 (enrdf_load_stackoverflow)
JP (1) JP2014533502A (enrdf_load_stackoverflow)
KR (1) KR20140094007A (enrdf_load_stackoverflow)
CN (1) CN104093857A (enrdf_load_stackoverflow)
AU (1) AU2012337389A1 (enrdf_load_stackoverflow)
BR (1) BR112014011937A2 (enrdf_load_stackoverflow)
CA (1) CA2855953A1 (enrdf_load_stackoverflow)
HK (1) HK1199746A1 (enrdf_load_stackoverflow)
IL (1) IL232586A0 (enrdf_load_stackoverflow)
MX (1) MX338076B (enrdf_load_stackoverflow)
SG (1) SG11201402341QA (enrdf_load_stackoverflow)
WO (1) WO2013074163A1 (enrdf_load_stackoverflow)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015008985A1 (en) * 2013-07-15 2015-01-22 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
CN107735403B (zh) * 2015-04-16 2021-06-11 威廉马歇莱思大学 由辅助寡核苷酸物质对核酸杂交探针的化学计量调节
CN110016500A (zh) * 2018-01-10 2019-07-16 深圳闪量科技有限公司 表面探针定量pcr方法
AU2020388573A1 (en) * 2019-11-21 2022-06-23 10X Genomics, Inc, Spatial analysis of analytes
CN111808989A (zh) * 2020-06-18 2020-10-23 重庆浦洛通基因医学研究院有限公司 一种冠状病毒/流感病毒/鼻病毒核酸联合检测试剂盒及使用方法
CN116438318A (zh) * 2020-10-15 2023-07-14 基因泰克公司 多重靶标结合候选筛选分析

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0601834A1 (en) * 1992-12-07 1994-06-15 Wisconsin Alumni Research Foundation Method of cleaving a nucleic acid molecule
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
US20080241838A1 (en) * 2006-12-29 2008-10-02 Applera Corporation, Applied Biosystems Group Methods and systems for detecting nucleic acids

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003042353A2 (en) * 2001-07-17 2003-05-22 Stratagene Methods for detection of a target nucleic acid by capture using multi-subunit probes
WO2005010199A2 (en) * 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout
WO2005068660A1 (en) * 2003-12-19 2005-07-28 Beckman Coulter, Inc. Solid-phase multiplexed invader assay
US9115389B2 (en) * 2006-06-30 2015-08-25 Rosetta Genomics Ltd. Method for detecting a target nucleic acid comprising two portions using probes having a first portion complementary to the first portion of the target nucleic acid and a second portion substantially complementary to the second portion of the target nucleic acid
EP2077336A1 (en) * 2007-12-19 2009-07-08 Koninklijke Philips Electronics N.V. Device and method for parallel quantitative analysis of multiple nucleic acids
US20120040853A1 (en) * 2008-11-21 2012-02-16 Koninklijke Philips Electronics N.V. Real time multiplex pcr detection on solid surfaces using double stranded nucleic acid specific dyes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0601834A1 (en) * 1992-12-07 1994-06-15 Wisconsin Alumni Research Foundation Method of cleaving a nucleic acid molecule
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
US20080241838A1 (en) * 2006-12-29 2008-10-02 Applera Corporation, Applied Biosystems Group Methods and systems for detecting nucleic acids

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BERNARD JUSKOWIAK: "Nucleic acid-based fluorescent probes and their analytical potential", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, SPRINGER, BERLIN, DE, vol. 399, no. 9, 29 October 2010 (2010-10-29), pages 3157 - 3176, XP019885328, ISSN: 1618-2650, DOI: 10.1007/S00216-010-4304-5 *
MATSUBARA Y ET AL: "Microchamber array based DNA quantification and specific sequence detection from a single copy via PCR in nanoliter volumes", BIOSENSORS AND BIOELECTRONICS, ELSEVIER BV, NL, vol. 20, no. 8, 15 February 2005 (2005-02-15), pages 1482 - 1490, XP027619503, ISSN: 0956-5663, [retrieved on 20050215] *
See also references of WO2013074163A1 *
SHALON D ET AL: "A DNA MICROARRAY SYSTEM FOR ANALYZING COMPLEX DNA SAMPLES USING TWO-COLOR FLUORESCENT PROBE HYBRIDIZATION", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, WOODBURY, NY, US, vol. 6, no. 7, 1 July 1996 (1996-07-01), pages 639 - 645, XP009007958, ISSN: 1088-9051 *

Also Published As

Publication number Publication date
BR112014011937A2 (pt) 2017-05-30
SG11201402341QA (en) 2014-06-27
WO2013074163A1 (en) 2013-05-23
CA2855953A1 (en) 2013-05-23
MX2014005912A (es) 2014-09-04
JP2014533502A (ja) 2014-12-15
KR20140094007A (ko) 2014-07-29
CN104093857A (zh) 2014-10-08
MX338076B (es) 2016-04-01
EP2780479A1 (en) 2014-09-24
AU2012337389A1 (en) 2014-06-19
HK1199746A1 (en) 2015-07-17
IL232586A0 (en) 2014-06-30

Similar Documents

Publication Publication Date Title
IL227865A0 (en) Quantitative, highly multiplexed detection of nucleic acids
IL255724A (en) Malware analysis system
IL230610A0 (en) Methods for detecting nucleotide change
ZA201205891B (en) Dna polymerases
IL231983A0 (en) Dna containing tricyclo-phosphorothioate
ZA201406410B (en) Detection of nucleic acids
EP3425063C0 (en) PROCEDURE FOR DETECTING ANALYTES
EP2833144A4 (en) METHOD OF DETECTING BIOLOGICAL SUBSTANCE
EP2776574B8 (en) Methods for determining nucleotide sequence repeats
IL239258B (en) Multiplexed nucleic acid detection methods
GB201118636D0 (en) Nucleotide sequence
PT2736340T (pt) Biocontrolo de nemátodos
IL232586A0 (en) Highly multiplexed quantitative detection of nucleic acids
GB201215571D0 (en) Assay method
ZA201401400B (en) Assay for detection of jc virus dna
EP2786150A4 (en) DETECTION OF MULTIPLE ANALYTES
GB201118026D0 (en) Biosensor
EP2843047A4 (en) NUCLEINSÄUREDETEKTIONSVERFAHREN
GB201210756D0 (en) Analysis of small RNA
GB201221011D0 (en) Assay method
GB201107286D0 (en) Nucleic acid preparation
AU2012902218A0 (en) Detection of nucleic acids
AU2012900624A0 (en) Detection of Nucleic Acids
GB201119515D0 (en) Assay method
GB201107305D0 (en) Assay method

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20140529

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

RIN1 Information on inventor provided before grant (corrected)

Inventor name: SCABOO, KRIS

Inventor name: TAFT, BRAD

Inventor name: MARTIN, PATRICK

Inventor name: LA, JASON

DAX Request for extension of the european patent (deleted)
RA4 Supplementary search report drawn up and despatched (corrected)

Effective date: 20150311

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 15/11 20060101ALI20150305BHEP

Ipc: C12Q 1/68 20060101AFI20150305BHEP

REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1199746

Country of ref document: HK

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20170301

REG Reference to a national code

Ref country code: HK

Ref legal event code: WD

Ref document number: 1199746

Country of ref document: HK