CN104068023B - A kind of agricultural bactericidal composition - Google Patents
A kind of agricultural bactericidal composition Download PDFInfo
- Publication number
- CN104068023B CN104068023B CN201410323860.9A CN201410323860A CN104068023B CN 104068023 B CN104068023 B CN 104068023B CN 201410323860 A CN201410323860 A CN 201410323860A CN 104068023 B CN104068023 B CN 104068023B
- Authority
- CN
- China
- Prior art keywords
- fludioxonil
- ningnanmycin
- grams per
- per liter
- bactericidal composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 68
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 20
- 239000005781 Fludioxonil Substances 0.000 claims abstract description 44
- MUJOIMFVNIBMKC-UHFFFAOYSA-N fludioxonil Chemical group C=12OC(F)(F)OC2=CC=CC=1C1=CNC=C1C#N MUJOIMFVNIBMKC-UHFFFAOYSA-N 0.000 claims abstract description 44
- AMNAZJFEONUVTD-KEWDHRJRSA-N (2s,3s,4s,5r,6r)-6-(4-amino-2-oxopyrimidin-1-yl)-4,5-dihydroxy-3-[[(2s)-3-hydroxy-2-[[2-(methylamino)acetyl]amino]propanoyl]amino]oxane-2-carboxamide Chemical compound O1[C@H](C(N)=O)[C@@H](NC(=O)[C@H](CO)NC(=O)CNC)[C@H](O)[C@@H](O)[C@@H]1N1C(=O)N=C(N)C=C1 AMNAZJFEONUVTD-KEWDHRJRSA-N 0.000 claims abstract description 43
- FEACDOXQOYCHKU-UHFFFAOYSA-N Gougerotin Natural products CNCC(=O)NC1=NC(=O)N(C=C1)C2OC(C(O)C(NC(=O)C(N)CO)C2O)C(=O)N FEACDOXQOYCHKU-UHFFFAOYSA-N 0.000 claims abstract description 43
- 239000004480 active ingredient Substances 0.000 claims abstract description 33
- 239000000375 suspending agent Substances 0.000 claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 13
- 241000209140 Triticum Species 0.000 claims abstract description 12
- 235000021307 Triticum Nutrition 0.000 claims abstract description 12
- 240000008067 Cucumis sativus Species 0.000 claims abstract description 10
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims abstract description 10
- 239000011248 coating agent Substances 0.000 claims abstract description 9
- 239000000725 suspension Substances 0.000 claims abstract description 9
- 239000003094 microcapsule Substances 0.000 claims abstract description 8
- 239000004562 water dispersible granule Substances 0.000 claims abstract description 8
- 238000009736 wetting Methods 0.000 claims abstract description 8
- 238000009472 formulation Methods 0.000 claims description 17
- 238000000034 method Methods 0.000 abstract description 28
- 239000002131 composite material Substances 0.000 abstract description 15
- 230000002195 synergetic effect Effects 0.000 abstract description 11
- 239000003905 agrochemical Substances 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 22
- 239000002270 dispersing agent Substances 0.000 description 20
- 239000003814 drug Substances 0.000 description 18
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 16
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 15
- 201000010099 disease Diseases 0.000 description 15
- 230000008569 process Effects 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 230000001988 toxicity Effects 0.000 description 14
- 231100000419 toxicity Toxicity 0.000 description 14
- 239000003795 chemical substances by application Substances 0.000 description 13
- 239000000080 wetting agent Substances 0.000 description 11
- 239000002904 solvent Substances 0.000 description 10
- 239000003755 preservative agent Substances 0.000 description 9
- 230000002335 preservative effect Effects 0.000 description 9
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 8
- 239000004299 sodium benzoate Substances 0.000 description 8
- 235000010234 sodium benzoate Nutrition 0.000 description 8
- 229920001285 xanthan gum Polymers 0.000 description 8
- 239000002775 capsule Substances 0.000 description 7
- 239000013530 defoamer Substances 0.000 description 7
- 239000000178 monomer Substances 0.000 description 7
- 239000002562 thickening agent Substances 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 6
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 6
- 239000004205 dimethyl polysiloxane Substances 0.000 description 6
- YDEXUEFDPVHGHE-GGMCWBHBSA-L disodium;(2r)-3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Na+].[Na+].COC1=CC=CC(C[C@H](CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O YDEXUEFDPVHGHE-GGMCWBHBSA-L 0.000 description 6
- 239000000839 emulsion Substances 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
- 239000000575 pesticide Substances 0.000 description 6
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 6
- 230000003449 preventive effect Effects 0.000 description 6
- 241000123650 Botrytis cinerea Species 0.000 description 5
- 241001290235 Ceratobasidium cereale Species 0.000 description 5
- 229920005551 calcium lignosulfonate Polymers 0.000 description 5
- RYAGRZNBULDMBW-UHFFFAOYSA-L calcium;3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Ca+2].COC1=CC=CC(CC(CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O RYAGRZNBULDMBW-UHFFFAOYSA-L 0.000 description 5
- 239000000945 filler Substances 0.000 description 5
- 238000011835 investigation Methods 0.000 description 5
- LCRMGUFGEDUSOG-UHFFFAOYSA-N naphthalen-1-ylsulfonyloxymethyl naphthalene-1-sulfonate;sodium Chemical compound [Na].C1=CC=C2C(S(=O)(OCOS(=O)(=O)C=3C4=CC=CC=C4C=CC=3)=O)=CC=CC2=C1 LCRMGUFGEDUSOG-UHFFFAOYSA-N 0.000 description 5
- 244000052769 pathogen Species 0.000 description 5
- 230000001717 pathogenic effect Effects 0.000 description 5
- 230000000361 pesticidal effect Effects 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 239000004372 Polyvinyl alcohol Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 4
- 239000003086 colorant Substances 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- -1 oleoyl amine Chemical class 0.000 description 4
- 229920002689 polyvinyl acetate Polymers 0.000 description 4
- 235000019422 polyvinyl alcohol Nutrition 0.000 description 4
- 229920002451 polyvinyl alcohol Polymers 0.000 description 4
- 238000010998 test method Methods 0.000 description 4
- 239000005995 Aluminium silicate Substances 0.000 description 3
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 description 3
- 235000012211 aluminium silicate Nutrition 0.000 description 3
- 239000003899 bactericide agent Substances 0.000 description 3
- 239000000306 component Substances 0.000 description 3
- 239000007884 disintegrant Substances 0.000 description 3
- 235000011187 glycerol Nutrition 0.000 description 3
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000223600 Alternaria Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 244000060011 Cocos nucifera Species 0.000 description 2
- 235000013162 Cocos nucifera Nutrition 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000221785 Erysiphales Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- 230000002528 anti-freeze Effects 0.000 description 2
- 239000012752 auxiliary agent Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 235000015895 biscuits Nutrition 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000012948 isocyanate Substances 0.000 description 2
- 238000010030 laminating Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000010534 mechanism of action Effects 0.000 description 2
- 235000010603 pastilles Nutrition 0.000 description 2
- 238000007747 plating Methods 0.000 description 2
- 229920005646 polycarboxylate Polymers 0.000 description 2
- 229920001228 polyisocyanate Polymers 0.000 description 2
- 239000005056 polyisocyanate Substances 0.000 description 2
- 229920006389 polyphenyl polymer Polymers 0.000 description 2
- 230000003716 rejuvenation Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000222195 Ascochyta Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241001530056 Athelia rolfsii Species 0.000 description 1
- 208000031968 Cadaver Diseases 0.000 description 1
- 241000371644 Curvularia ravenelii Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 241000233679 Peronosporaceae Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241001361634 Rhizoctonia Species 0.000 description 1
- 241000813090 Rhizoctonia solani Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000000853 biopesticidal effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 244000000037 crop pathogen Species 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- XUZHLZDRCCUWEV-UHFFFAOYSA-N formaldehyde;methyl naphthalene-1-sulfonate Chemical compound O=C.C1=CC=C2C(S(=O)(=O)OC)=CC=CC2=C1 XUZHLZDRCCUWEV-UHFFFAOYSA-N 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- PSZYNBSKGUBXEH-UHFFFAOYSA-M naphthalene-1-sulfonate Chemical compound C1=CC=C2C(S(=O)(=O)[O-])=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-M 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 150000003233 pyrroles Chemical class 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 210000002845 virion Anatomy 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of agricultural bactericidal composition, its active ingredient is fludioxonil and Ningnanmycin, and both mass ratioes are 40:1-1:40, and all the other are allow in agricultural chemicals to use and acceptable auxiliary element.The present composition can be prepared into suspending agent, microcapsule suspending agent, suspension seed-coating agent, wetting powder or water dispersible granules by known method.Fludioxonil and Ningnanmycin composite with certain proportion after there is obvious synergistic effect, be mainly used in prevent and treat wheat sharp eyespot, gray mold of cucumber.
Description
Technical field
The invention belongs to technical field of pesticide combination, relate to a kind of agricultural bactericidal composition, the application of especially a kind of agricultural bactericidal composition containing fludioxonil and control corps diseases thereof.
Background technology
Fludioxonil (Fludioxonil), molecular formula: C
26h
23f
2nO
4, be a kind of new pyrrole class non-internal-absorting wide-spectrum bactericide of Syngenta Co., Ltd's development and production, its mechanism of action is the transferase by suppressing thalline glucose phosphorylated relevant, and the mycelial growth of energy Antifungi, causes germ dead.To microbial diseases of cause of disease such as Alternaria, Ascochyta, aspergillus, Fusarium, Helminthosporium, Rhizoctonia and Penicilliums, there is well protection and therapeutic action, for preventing and treating snow rotten Fusariumsp, wheat black rot, rhizoctonia solani etc.
In recent years due to single drug and other unscientific application method, various crop pathogen has created pesticide resistance as downy mildew, powdery mildew, anthracnose, banded sclerotial blight etc., becomes a great problem of chemical control.Because pathogen produces resistance, pharmacy control efficacy declines, and causes frequent dispenser, causes peasant burden to increase the weight of and increasing environmental pollution.In order to meet people to food security and the more and more higher requirement of living environment, delay the drug-fast generation of pathogen, extend the service life of product, the synergistic composition of existing pesticide species has important Development volue.
Summary of the invention
The object of the invention is to: a kind of bactericidal composition being applicable to agriculturally production and application is provided, and contribute to reducing dosage, delay pathogen and produce resistance and reduce use cost.
For overcoming the deficiencies in the prior art, inventor is tested by a large amount of compound screening, find fludioxonil and Ningnanmycin composite with certain proportion after, to wheat sharp eyespot and gray mold of cucumber, there is obvious synergistic effect.
Ningnanmycin (ningnanmycin), molecular formula: C
16h
23o
8n
7being the innovation biopesticide succeeded in developing by Chengdu Inst. of Biology, Chinese Academy of Sciences, is Cystosine nucleocide type antibiotic, by extending the incubation period of germ, break virus mitochondrial structure, reduces virion concentration and regulates organism metabolism thus strengthen plant resistance against diseases and immunity.To diseases prevention and the obvious effect of increasing production of the multiple diseases such as tomato virus disease, rice seedling blight, alternaria leaf spot of apple, powdery mildew of cucumber.
Technical solution of the present invention is: a kind of agricultural bactericidal composition, it is characterized in that active ingredient is fludioxonil and Ningnanmycin, and the mass ratio of two active ingredients is 40:1-1:40, and preferred mass is than being 10:1-1:20.
Above-mentioned active component fludioxonil, Ningnanmycin are all bactericide well known in the art, can be buied by various commercial channel.
In bactericidal composition of the present invention, the mass accumulation percentage composition of active ingredient is 2%-55%, all the other are solvent, auxiliary agent, carrier and other be of value to the active ingredient auxiliary element that plays of stable and drug effect in the formulation.All auxiliary elements are all conventional in pesticidal preparations or allow to use, and be not particularly limited, concrete composition and consumption are determined by simple experiment according to recipe requirements.
Bactericidal composition of the present invention can be prepared into any one formulation of applicable agricultural use by known method, good formulation has suspending agent, microcapsule suspending agent, suspension seed-coating agent, wetting powder or water dispersible granules.
Composition described in the invention can provide with finished product preparation form, and namely in composition, each material mixes; Also can provide with single dose combining form, directly mix in described ratio in bucket or tank before using, be then diluted to required concentration.
Bactericidal composition of the present invention may be used for preventing and treating the numerous diseases on cereal, wheat class, beans and field vegetables crop, is particularly useful for control wheat sharp eyespot and gray mold of cucumber.Composition of the present invention can be used by common method, and as seed treatment, filling root, spray, spread fertilizer over the fields or be fuming, its amount of application is fixed with weather condition, Crop growing stage and disease occurrence degree.
Compared with prior art, the beneficial effect that the present invention produces is: (1) composition synergistic effect is obvious, and preventive effect significantly improves compared with single dose; (2), after drug effect improves, reduce field dosage and use cost, decrease residue of pesticide and environmental pollution; (3) composition is made up of the active ingredient of different mechanism of action, and action site increases, and is conducive to overcoming and delay germ and produces resistance.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, the following specific embodiment of the present invention is described, but the present invention is limited to absolutely not these examples.
Being undertaken composite by the pesticide active ingredient of different types of structure, is current development novel pesticide preparation and effective measures of preventing and treating agriculturally resistance germ.After the pestsides synthesis of different cultivars, usually show three kinds of type of action, i.e. summation action, synergistic effect and antagonism, but be specially which kind of effect, just can must be known by lot of experiments.Composite rear synergy is significantly filled a prescription, and can improve actual control efficiency, reduces dosage, and contributing to delaying germ and produce resistance, is the important means of integrated control disease.
Inventor, by a large amount of screening tests, finds fludioxonil and Ningnanmycin is composite has significant synergistic function to wheat sharp eyespot and gray mold of cucumber, and is not only the simple addition (referring to biologicall test example) of two kinds of medicaments.
Biologicall test example 1: fludioxonil and the composite Toxicity Determination to rhizoctonia cerealis of Ningnanmycin
Subjects: rhizoctonia cerealis (Rhizoctonia cerealis Van der Hoeven), is collected in field, the bacterial classification of rejuvenation of transferring through laboratory separation and 3 times.
Test method: test is with reference to " farm-chemical indoor determination test rule NY/T1156.2-2006 and 1156.6-2006 ", and this test adopts growth rate method (Plating).On the basis of preliminary experiment, on aseptic operating platform, according to test process, the sterilising medium melted in advance is quantitatively placed in aseptic conical flask, from low concentration to high concentration, get 5mL liquid successively join respectively the hot medium of 45mL (PDA medium is housed, 45-50 DEG C) conical flask in, after shaking up, pour rapidly diameter 90mm glass culture dish into, band medicine medium 10mL poured into by each culture dish.Horizontal rest, makes flat board after cooling.Each concentration five repetition.Blank is made with the process not containing medicament effective ingredient.
By cultured pathogen, aseptically use the sterilizing card punch of diameter 5mm, cut bacterium cake from colony edge, with inoculator, pure culture biscuits involvng inoculation is dull and stereotyped central in pastille, facing down having one of mycelia and medium laminating, covering ware lid.Above all operations all carries out sterile working at superclean bench.
Be placed on after process in the constant temperature aseptic culture case of 26 ± 0.5 DEG C and cultivate, take out after 2d.Adopt right-angled intersection method to measure the colony diameter (in units of mm) of each process respectively, calculate the mean value of colony diameter.Do regression analysis with the preventive effect probability value of the valid density logarithm value of each test medicine and correspondence, obtain concentration EC in the suppression of each medicament
50, 90% inhibition concentration EC
90and the confidence limit of 95%.The toxicity index of each medicament and the co-toxicity coefficient (CTC value) of mixture is calculated according to the abundant method of Sun Yun.
Actual measurement toxicity index (ATI)=(standard agent EC
50/ reagent agent EC
50) × 100
In theoretical toxicity index (TTI)=A medicament toxicity index × mixture A percentage composition+B medicament toxicity index × mixture in the percentage composition of B
Co-toxicity coefficient (CTC)=[the theoretical toxicity index (TTI) of mixture actual measurement toxicity index (ATI)/mixture] × 100
When CTC≤80, then composition shows as antagonism, and as 80 < CTC < 120, then composition shows as summation action, and when CTC >=120, then composition shows as synergistic effect.Joint toxicity measuring the results are shown in Table 1.
Table 1 fludioxonil and the composite Toxicity Determination result to rhizoctonia cerealis of Ningnanmycin
In table 1, result shows, when fludioxonil and Ningnanmycin are between proportioning 40:1-1:40, have synergistic effect to rhizoctonia cerealis, co-toxicity coefficient is all more than 120, when proportioning is between 10:1-1:20, synergistic effect particularly evident (co-toxicity coefficient is greater than 160).
Biologicall test example 2: fludioxonil and the composite Toxicity Determination to botrytis cinerea pers of Ningnanmycin
Subjects: botrytis cinerea pers (Botrytis cinerea), is provided by Agricultural University Of South China's bactericide laboratory, through the bacterial classification of research institute of company raw survey group sterilizing chamber 3 switching rejuvenation.
Test method: test is with reference to " farm-chemical indoor determination test rule NY/T1156.2-2006 and 1156.6-2006 ", and this test adopts growth rate method (Plating).On the basis of preliminary experiment, on aseptic operating platform, according to test process, the sterilising medium melted in advance is quantitatively placed in aseptic conical flask, from low concentration to high concentration, get 5mL liquid successively join respectively the hot medium of 45mL (PDA medium is housed, 45-50 DEG C) conical flask in, after shaking up, pour rapidly diameter 90mm glass culture dish into, band medicine medium 10mL poured into by each culture dish.Horizontal rest, makes flat board after cooling.Each concentration five repetition.Blank is made with the process not containing medicament active ingredient.
By cultured pathogen, aseptically use the sterilizing card punch of diameter 4mm, cut bacterium cake from colony edge, with inoculator, pure culture biscuits involvng inoculation is dull and stereotyped central in pastille, facing down having one of mycelia and medium laminating, covering ware lid.Above all operations all carries out sterile working at superclean bench.
Be placed on after process in the constant temperature aseptic culture case of 26 ± 0.5 DEG C and cultivate, take out after 6d.Adopt right-angled intersection method to measure the colony diameter (in units of millimeter) of each process respectively, calculate the mean value of colony diameter.
The logarithm value processing effective ingredient concentration (μ g/mL) with each is independent variable X, the probit value that inhibiting rate changes into is dependent variable Y, use expert data statistical software DPS process, obtain A and B in virulence regression equation formula (Y=A+BX); Concentration EC in suppression
50; 90% inhibition concentration EC
90; EC
50the confidence limit of 95%; Standard error SE (B).The toxicity index of each medicament and the co-toxicity coefficient (CTC value) of mixture is calculated according to the abundant method of Sun Yun.
Co-toxicity coefficient computational methods are with above-described embodiment 1.
Joint toxicity measuring the results are shown in Table 2.
Table 2 fludioxonil and the composite Toxicity Determination result to botrytis cinerea pers of Ningnanmycin
In table 2, result shows, when fludioxonil and Ningnanmycin are between proportioning 40:1-1:40, has synergistic effect to botrytis cinerea pers, and co-toxicity coefficient is all more than 120.When proportioning is between 10:1-1:20, synergistic effect more obvious (co-toxicity coefficient is greater than 170).
The present composition can be prepared into any one formulation of applicable agricultural use by known method, reasonable formulation has suspending agent, microcapsule suspending agent, suspension seed-coating agent, wetting powder or water dispersible granules.
The following stated is only the good embodiment of the present invention, only in order to explain the present invention, therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.In all formulas of the embodiment of the present invention, percentage is mass percent, the amount of the amount of active ingredient to be industrial goods conversion the be sterling of 100% content.
Wetting powder embodiment
The ratio in formula such as pesticide activity component, various auxiliary agent and filler is fully mixed, after ultra-fine pulverizer disintegrating, obtains wetting powder product.
Example of formulations 1:41% fludioxonil Ningnanmycin wetting powder
Formula composition: active ingredient fludioxonil 40%, active ingredient Ningnanmycin 1%, lauryl sodium sulfate (wetting agent) 6%, naphthalene sulfonate (diffusant) 5%, sodium lignin sulfonate (dispersant) 7%, kaolin (filler) complements to 100%.
Suspending agent embodiment
The ratio of each components such as pesticide activity component, dispersant, wetting agent, thickener, defoamer, preservative, antifreeze and water in formula is mixed, after grinding and/or high speed shear, obtains suspending agent product.
Example of formulations 2:31% fludioxonil Ningnanmycin suspending agent
Formula composition: active ingredient fludioxonil 30%, active ingredient Ningnanmycin 1%, methyl naphthalene sulfonate formaldehyde condensate (wetting agent) 10%, xanthans (thickener) 1%, 20% aqueous emulsion of dimethyl polysiloxane fluid (defoamer) 0.4%, sodium lignin sulfonate (dispersant) 3%, glycerine (antifreeze) 4%, Sodium Benzoate (preservative) 0.25%, water (carrier) complements to 100%.
Formulation Example 3:12% fludioxonil Ningnanmycin suspending agent
Formula composition: active ingredient Ningnanmycin 10%, active ingredient fludioxonil 2%, calcium lignosulfonate (dispersant) 3.5%, sodium methylene bis-naphthalene sulfonate (dispersant) 2%, pull open powder (wetting agent) 1%, 0% aqueous emulsion of dimethyl polysiloxane fluid (defoamer) 0.4%, ethylene glycol (antifreezing agent) 4%, xanthans (thickener) 0.15%, Sodium Benzoate (preservative) 0.15%, water (carrier) surplus.
Suspension seed-coating agent embodiment
Formulation Example 4:6% fludioxonil Ningnanmycin suspension seed-coating agent
Formula composition: active ingredient fludioxonil 5%, active ingredient Ningnanmycin 1%, calcium lignosulfonate (dispersant) 2.5%, sodium methylene bis-naphthalene sulfonate (dispersant) 1%, pull open powder (wetting agent) 2%, 20% aqueous emulsion of dimethyl polysiloxane fluid (defoamer) 0.4%, ethylene glycol (antifreezing agent) 4%, xanthans (thickener) 0.25%, Sodium Benzoate (preservative) 0.25%, PVAC polyvinylalcohol (film forming agent) 0.3%, solvent red (coloring agent) 1%, water (carrier) surplus.
Formulation Example 5:105 grams per liter fludioxonil Ningnanmycin suspension seed-coating agent
Formula composition: active ingredient fludioxonil 5 grams per liter, active ingredient Ningnanmycin 100 grams per liter, calcium lignosulfonate (dispersant) 30 grams per liter, sodium methylene bis-naphthalene sulfonate (dispersant) 20 grams per liter, pull open powder (wetting agent) 30 grams per liter, 20% aqueous emulsion of dimethyl polysiloxane fluid (defoamer) 4 grams per liter, ethylene glycol (antifreezing agent) 35 grams per liter, xanthans (thickener) 1.5 grams per liter, Sodium Benzoate (preservative) 1.5 grams per liter, PVAC polyvinylalcohol (film forming agent) 2.5 grams per liter, solvent red (coloring agent) 10 grams per liter, water (carrier) surplus.
Formulation Example 6:66 grams per liter fludioxonil Ningnanmycin suspension seed-coating agent
Formula composition: active ingredient Ningnanmycin 60 grams per liter, active ingredient fludioxonil 6 grams per liter, calcium lignosulfonate (dispersant) 35 grams per liter, sodium methylene bis-naphthalene sulfonate (dispersant) 35 grams per liter, pull open powder (wetting agent) 40 grams per liter, 20% aqueous emulsion of dimethyl polysiloxane fluid (defoamer) 4 grams per liter, ethylene glycol (antifreezing agent) 40 grams per liter, xanthans (thickener) 1.8 grams per liter, Sodium Benzoate (preservative) 1.8 grams per liter, PVAC polyvinylalcohol (film forming agent) 2.5 grams per liter, solvent red (coloring agent) 10 grams per liter, water (carrier) surplus.
Formulation Example 7:82 grams per liter fludioxonil Ningnanmycin suspension seed-coating agent
Formula composition: active ingredient Ningnanmycin 80 grams per liter, active ingredient fludioxonil 2 grams per liter, calcium lignosulfonate (dispersant) 25 grams per liter, sodium methylene bis-naphthalene sulfonate (dispersant) 30 grams per liter, pull open powder (wetting agent) 35 grams per liter, 20% aqueous emulsion of dimethyl polysiloxane fluid (defoamer) 4 grams per liter, ethylene glycol (antifreezing agent) 36 grams per liter, xanthans (thickener) 1.6 grams per liter, Sodium Benzoate (preservative) 1.6 grams per liter, PVAC polyvinylalcohol (film forming agent) 2.8 grams per liter, solvent red (coloring agent) 10 grams per liter, water (carrier) surplus.
Microcapsule suspending agent embodiment
Pesticide activity component, solvent, capsule skin monomer are mixed mutually, enters compounding kettle and stir, make oil phase, more mixed with water through shearing dispersion Post RDBMS encystation, obtained microcapsule suspending agent product.
Formulation Example 8:2% fludioxonil Ningnanmycin microcapsule suspending agent
Formula composition: active ingredient Ningnanmycin 1%, active ingredient fludioxonil 1%, solvent naphtha S-200 (solvent) 10%, sodium lignin sulfonate (dispersant) 3.5%, coconut oleoyl amine (capsule skin monomer) 4%, poly methylene poly phenyl poly isocyanate (capsule skin monomer) 1.5%, toluene di-isocyanate(TDI) (capsule skin monomer) 1.5%, xanthans (stabilizing agent) 0.2%, Sodium Benzoate (preservative) 0.2%, glycerine (antifreezing agent) 3%, water (carrier) is supplied.
Formulation Example 9:62 grams per liter fludioxonil Ningnanmycin microcapsule suspending agent
Formula composition: active ingredient Ningnanmycin 60 grams per liter, active ingredient fludioxonil 2 grams per liter, solvent naphtha S-200 (solvent) 90 grams per liter, sodium lignin sulfonate (dispersant) 30 grams per liter, coconut oleoyl amine (capsule skin monomer) 40 grams per liters, poly methylene poly phenyl poly isocyanate (capsule skin monomer) 12 grams per liters, toluene di-isocyanate(TDI) (capsule skin monomer) 16 grams per liters, xanthans (stabilizing agent) 1.5 grams per liter, Sodium Benzoate (preservative) 1.5 grams per liter, glycerine (antifreezing agent) 30 grams per liter, water (carrier) is supplied.
Water dispersible granules embodiment
Pesticide activity component, dispersant, wetting agent, disintegrant and the filler ratio in formula is mixed, becomes wetting powder through air-flow crushing, then add a certain amount of water mixing extruder grain, after drying screening, obtain water dispersible granules product.
Formulation Example 10:42% fludioxonil Ningnanmycin water dispersible granules
Formula composition: active ingredient fludioxonil 40%, active ingredient Ningnanmycin 2%, polycarboxylate (dispersant) 5%, sodium lignin sulfonate (dispersant) 10%, lauryl sodium sulfate (wetting agent) 4%, ammonium sulfate (disintegrant) 15%, kaolin (filler) complements to 100%.
Formulation Example 11:55% fludioxonil Ningnanmycin water dispersible granules
Formula composition: active ingredient fludioxonil 50%, active ingredient Ningnanmycin 5%, polycarboxylate (dispersant) 5%, sodium lignin sulfonate (dispersant) 10%, lauryl sodium sulfate (wetting agent) 4%, ammonium sulfate (disintegrant) 15%, kaolin (filler) complements to 100%.
In order to further illustrate the advantage of the technology of the present invention formula scheme, the following concrete field trial embodiment of the present invention is illustrated.
Field trial embodiment 1: fludioxonil and Ningnanmycin composite control wheat sharp eyespot field control effectiveness test
Test and carry out in Shangshui County, Zhoukou City of Henan Province in March, 2013, soil fertility is medium, and front stubble is corn, and experimental field wheat sharp eyespot over the years occurs serious.Test medicine process, using dosage and preventive effect refer to table 3, often process 4 times and repeat, plot area 20m
2, Gong44Ge community, random district group arranges.
Test method: 1) adopt conventional spray-on process, carries out first time dispenser at wheat sharp eyespot their early stage, and according to disease development, every dispenser again in 7-10 days, dispenser 3 times, separately establishes clear water to be treated to blank altogether.2) dress seed: the medicament thin up first will weighed up, amount of water 1.5L/100kg seed, then slowly pours into liquid on the seed got ready, stirs, and dries the sowing of rear this day.
Investigation method: every community diagonal 5 samplings, often some investigation 100 strains, after last medicine, 14 days investigation disease indexs and diseased plant rate, calculate control efficiency.
Table 3 fludioxonil and Ningnanmycin composite control wheat sharp eyespot field control effectiveness test result
Field trial embodiment 2: fludioxonil and Ningnanmycin composite control gray mold of cucumber field control effectiveness test
Test and carry out in growing vegetables base, Yanqing County, Beijing in April, 2013, soil fertility is medium, and experimental field gray mold of cucumber over the years occurs serious.Test medicine process, using dosage and preventive effect refer to table 4, often process 4 times and repeat, plot area 15m
2, Gong44Ge community, random district group arranges.
Test method: 1) adopt conventional spray-on process, in the first time dispenser of disease initial stage of origination, according to disease occurrence degree every dispenser in 7 days 1 time, dispenser 3 times, separately establishes clear water to be treated to blank altogether.2) dress seed: the medicament thin up first will weighed up, amount of water 1.5L/100kg seed, then slowly pours into liquid on the seed got ready, stirs, and dries the sowing of rear this day.
Investigation method: every community diagonal 5 samplings, often some sampling 2 strains, the investigation complete stool fruit incidence of disease, records disease fruit tree and sick progression, calculates the control efficiency of each medicament.
Table 4 fludioxonil and Ningnanmycin composite control gray mold of cucumber field control effectiveness test result
Above-mentioned wheat sharp eyespot and gray mold of cucumber field control effectiveness test result all show, fludioxonil and Ningnanmycin composite with certain proportion after, effective dose is all starkly lower than the simple addition of two kinds of single dose effective doses, and composite after preventive effect be obviously better than single dose preventive effect, illustrate that technical scheme of the present invention is practicable.
Claims (5)
1. an agricultural bactericidal composition, is characterized in that: the active ingredient of described bactericidal composition is fludioxonil and Ningnanmycin, and both mass ratioes are 40:1-1:40.
2. bactericidal composition according to claim 1, is characterized in that: the mass ratio of fludioxonil and Ningnanmycin is 10:1-1:20.
3. bactericidal composition according to claim 1, is characterized in that: the gross mass percentage composition of active ingredient is 2%-55%.
4. bactericidal composition according to claim 1 and 2, is characterized in that: the formulation of described bactericidal composition is suspending agent, microcapsule suspending agent, suspension seed-coating agent, wetting powder or water dispersible granules.
5. bactericidal composition described in claim 1 is applied to the purposes of control wheat sharp eyespot and gray mold of cucumber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410323860.9A CN104068023B (en) | 2014-07-09 | 2014-07-09 | A kind of agricultural bactericidal composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410323860.9A CN104068023B (en) | 2014-07-09 | 2014-07-09 | A kind of agricultural bactericidal composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104068023A CN104068023A (en) | 2014-10-01 |
| CN104068023B true CN104068023B (en) | 2015-09-09 |
Family
ID=51589952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410323860.9A Active CN104068023B (en) | 2014-07-09 | 2014-07-09 | A kind of agricultural bactericidal composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104068023B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015160619A1 (en) * | 2014-04-16 | 2015-10-22 | Bayer Cropscience Lp | Compositions comprising ningnanmycin and a fungicide |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102067840A (en) * | 2011-02-17 | 2011-05-25 | 德强生物股份有限公司 | Compound pesticide containing triflumizole and ningnanmycin |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130324493A1 (en) * | 2012-06-01 | 2013-12-05 | North Carolina State University | Compositions comprising ningnanmycin and uses therefor |
-
2014
- 2014-07-09 CN CN201410323860.9A patent/CN104068023B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102067840A (en) * | 2011-02-17 | 2011-05-25 | 德强生物股份有限公司 | Compound pesticide containing triflumizole and ningnanmycin |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104068023A (en) | 2014-10-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102172240B (en) | A kind of bactericidal composition containing benziothiazolinone and methoxy acrylic bactericide | |
| CN105961399B (en) | Bactericidal composition and its application | |
| CN103947658B (en) | Thifluzamide-containing bactericidal composition | |
| CN103814909A (en) | Synergistic pesticidal composition | |
| CN101642104A (en) | Sterilization composition containing fenoxanil | |
| CN104255757A (en) | Compound bactericide | |
| CN104068023B (en) | A kind of agricultural bactericidal composition | |
| CN104351254B (en) | A kind of containing Nikemycin with the bactericidal composition of Fluoxastrobin | |
| CN104996450A (en) | Sterilization composite contained with pyribencarb | |
| CN104982451A (en) | Bactericidal composition containing pyribencarb | |
| CN102308817B (en) | Bactericidal composition | |
| CN102308835B (en) | Pesticide composition containing streptomycin sulfate and oxine-copper | |
| CN104542630A (en) | Antibacterial composition containing zinc thiazole and benziothiazolinone | |
| CN104026136B (en) | Agricultural fungicidal composition | |
| CN105052997B (en) | A kind of composition pesticide and bactericide containing toxic fluoride phosphate and antimicrobial fungicides | |
| CN104604897A (en) | Sterilization composition containing thifluzamide and fludioxonil | |
| CN103960261B (en) | Sterilization composition | |
| CN104012555A (en) | Agricultural sterilization composition | |
| CN104026152B (en) | A kind of agricultural bactericidal composition | |
| CN103975923B (en) | A kind of agricultural bactericidal composition | |
| CN102972402B (en) | Kasugamycin-containing pesticide composition | |
| CN103636670B (en) | Sterilization composition containing Dufulin and enestroburin | |
| CN102224824B (en) | Sterilization composition containing kresoxim-methyl and iprodione | |
| CN105340898A (en) | Sterilization composition containing penthiopyrad and fludioxonil | |
| CN104585197A (en) | Compound bactericide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 518102 No. 113 Reservoir Road, Baoan District, Guangdong, Shenzhen, Xixiang Patentee after: Shenzhen Novozon Crop Science Co.,Ltd. Country or region after: China Address before: 518102 No. 113 Reservoir Road, Baoan District, Guangdong, Shenzhen, Xixiang Patentee before: SHENZHEN NOPOSION AGROCHEMICALS Co.,Ltd. Country or region before: China |
|
| CP03 | Change of name, title or address | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240130 Address after: Unit 2310, 3rd Floor, Building 2, Fuzhou Strait Creative Industry Park, No.1 Jingong Road, Jianxin Town, Cangshan District, Fuzhou City, Fujian Province, 350000 Patentee after: Fujian Sino-Dashing Bioengineering Co.,Ltd. Country or region after: China Address before: 518102 No. 113 Reservoir Road, Baoan District, Guangdong, Shenzhen, Xixiang Patentee before: Shenzhen Novozon Crop Science Co.,Ltd. Country or region before: China |
|
| TR01 | Transfer of patent right |