CN102067840A - Compound pesticide containing triflumizole and ningnanmycin - Google Patents
Compound pesticide containing triflumizole and ningnanmycin Download PDFInfo
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- CN102067840A CN102067840A CN2011100396842A CN201110039684A CN102067840A CN 102067840 A CN102067840 A CN 102067840A CN 2011100396842 A CN2011100396842 A CN 2011100396842A CN 201110039684 A CN201110039684 A CN 201110039684A CN 102067840 A CN102067840 A CN 102067840A
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Abstract
The invention provides a pesticide combination, in particular to a compound pesticide, which contains ningnanmycin and triflumizole, wherein the ningnanmycin is purified or provided by a combination containing the ningnanmycin. In addition, the invention also provides pesticide preparations and the like prepared by the pesticide combination.
Description
Technical field
The invention belongs to pesticide field, particularly, the present invention relates to composition pesticide, it comprises Ningnanmycin and fluorine bacterium azoles, and wherein Ningnanmycin can be provided by the composition that contains Ningnanmycin.In addition, the invention still further relates to the pesticidal preparations etc. of above-mentioned agriculturally useful compositions preparation.
Background technology
Fluorine bacterium azoles (triflumizole) is a kind of imidazoles low toxicity efficient ergosterol demethylation inhibitor of Tso Tat Co., Ltd., Japan in exploitation in 1979; it belongs to flumizole heterocyclic nitrogen-group fungicide; have advantages such as efficient, low toxicity, low-residual; have protection and therapeutic action concurrently; can be widely used in prevent and treat multiple kinds of crops (as; water paddy and wheat, vegetables, melon and fruit) diseases such as powdery mildew, rust and tea tree anthrax; especially to powdery mildew have special efficacy (referring to Tan Chengxia etc. bactericide fluorine bacterium azoles synthetic and characterizing. agricultural chemicals; the 47th volume, the 7th phase: 497-499).Fluorine bacterium azoles is current mainly to be used with 30% triflumizole wettable powder in China, because price is comparatively expensive, uses large usage quantity separately, cost is higher, influenced the applying of China, especially, limited its use especially for the not rich peasant of China's wide geographic area.
Ningnanmycin (ningnanmycin) be a kind of from streptomyces noursei Xichang mutation (Streptomyces noursei var.xichangenis) CGMCC No.0194 isolated alkaline water dissolubility cytosine nucleoside antibiotic (its chemical constitution as shown in Equation 1), inhibited to Gram-negative and positive bacteria, especially the pathogen of the powdery mildews of plants such as bacterial blight of rice and wheat, cucumber, beans and flowers and tobacco mosaic virus etc. all there are good preventive and therapeutic effect (referring to No. the 93104287.9th, Chinese patent; To Gu Xi, etc. a kind of new farm antibiotics---Ningnanmycin. microorganism journal, the 35th volume, the 5th phase: 368-374).The substitute of chemistry agriculturals such as current withered peaceful as leaf, the phenazine oxide of Ningnanmycin is promoted the use of at the wide geographic area of China's the South and the North, has obtained huge economic and social benefit.
Especially the inventor has developed a kind of Ningnanmycin composition (referring to Chinese patent application 201010568126.0, denomination of invention is " a Ningnanmycin composition and method of making the same "), it comprises Ningnanmycin, also comprise formula as follows 2 compounds (promptly, compound with mass spectral analysis collection of illustrative plates shown in Figure 1) and formula 3 compounds (that is the compound that, has mass spectral analysis collection of illustrative plates shown in Figure 2):
This composition can disposablely prepare, and proportioning components is stable, though wherein Ningnanmycin only accounts for about 40% of described composition weight, still can play and the existing commercially available identical agricultural control efficiency of Ningnanmycin.
The inventor is through long-term and arduous research, find unexpectedly, when using when the use Ningnanmycin or with mould promotor composition in south and fluorine bacterium azoles (being mixed with Recompounded pesticide), has synergistic function, even the consumption of fluorine bacterium azoles in the minimizing Recompounded pesticide, also better control efficiency can be obtained, thereby the consumption of more expensive fluorine bacterium azoles can be reduced.
Summary of the invention
The technical problem to be solved in the present invention is to provide composition pesticide, and it comprises Ningnanmycin and fluorine bacterium azoles, has synergistic function, and can reduce the consumption of fluorine bacterium azoles, thereby reduce cost.
Particularly, in first aspect, the invention provides composition pesticide, it comprises Ningnanmycin and fluorine bacterium azoles.In this article, " agricultural chemicals " has implication known in the art, promptly uses and is used to prevent and/or treat the medicine of (that is, control) plant pest to plant.Wherein, Ningnanmycin can be a purifying, as being the commercially available former medicine of Ningnanmycin; Ningnanmycin can be to be provided by the composition that contains Ningnanmycin, for example the Chinese patent application 201010568126.0 described compositions that contain Ningnanmycin.
Ningnanmycin or contain the composition of Ningnanmycin and the weight ratio of fluorine bacterium azoles is 1-40: 1-60 in the composition of preferred first aspect present invention, be preferably 1-10: 10-50,2-6: 15-40 more preferably, most preferably be 4: 25, promptly, the weight ratio of Ningnanmycin and fluorine bacterium azoles is 1-40: 1-60, be preferably 1-10: 10-50, more preferably 2-6: 15-40 most preferably is 4: 25, perhaps, containing the composition of Ningnanmycin and the weight ratio of fluorine bacterium azoles is 1-40: 1-60, be preferably 1-10: 10-50, more preferably 2-6: 15-40 most preferably is 4: 25.
The composition that contains Ningnanmycin in the composition of first aspect present invention preferably comprises formula as follows 1 compound (promptly, Ningnanmycin), formula 2 compounds (promptly, compound with mass spectral analysis collection of illustrative plates shown in Figure 1) and formula 3 compounds (that is the compound that, has mass spectral analysis collection of illustrative plates shown in Figure 2):
The composition of first aspect present invention preferably is made up of composition that contains Ningnanmycin and fluorine bacterium azoles.Discover to have only about 40% through the inventor, can replace Ningnanmycin to use fully, control efficiency and the commercially available not significant difference of Ningnanmycin although contain the composition Ningnanmycin content of Ningnanmycin.
Contain in the composition of first aspect present invention and can comprise water in the composition of Ningnanmycin, also can not comprise water, preferably its water content is lower than 1% (w/w), preferably is lower than 0.5% (w/w), more preferably less than 0.4% (w/w), the moisture content as shown in the specific embodiment of the invention.The composition that contains Ningnanmycin in the composition of first aspect present invention preferably is made up of formula 1 compound, formula 2 compounds and formula 3 compounds and water.
The composition that contains Ningnanmycin in the composition of first aspect present invention preferably contains the Ningnanmycin greater than 35% (w/w).Particularly, contain in the composition of first aspect present invention in the composition of Ningnanmycin, the content of Ningnanmycin is greater than 35% (w/w), be preferably 40-50% (w/w), 40-45% (w/w) more preferably is as 40-42% (w/w), the content as shown in the specific embodiment of the invention.In this article, as do not add special instruction, content is all recently represented with weight percent.
The composition that contains Ningnanmycin in the composition of first aspect present invention preferably contains formula 2 compounds of 35-55% (w/w).Particularly, contain in the composition of first aspect present invention in the composition of Ningnanmycin, the content of formula 2 compounds is 35-55% (w/w), is preferably 40-48% (w/w), the content as shown in the specific embodiment of the invention.
The composition that contains Ningnanmycin in the composition of first aspect present invention preferably contains formula 3 compounds of 7-23% (w/w).Particularly, contain in the composition of first aspect present invention in the composition of Ningnanmycin, the content of formula 3 compounds is 7-23% (w/w), is preferably 11-18% (w/w), the content as shown in the specific embodiment of the invention.
The composition that contains Ningnanmycin in the composition of first aspect present invention can be also formulated together by obtaining (as synthetic or purification) formula 1 compound, formula 2 compounds and formula 3 compounds respectively, and prepare.But the composition that contains Ningnanmycin in the composition of first aspect present invention preferably prepares by the time that prolongs fermented and cultured.The composition gating that contains Ningnanmycin in the composition of first aspect present invention is spent the time that prolongs fermented and cultured and is prepared greater than 70 hours (more preferably greater than 70.5 hours, as 72 hours).Particularly, contain the preferably preparation by the following method of composition of Ningnanmycin in the composition of first aspect present invention:
(1) rejuvenation streptomyces noursei Xichang mutation;
(2) on solid culture medium, cultivate mycelium;
(3) carry out enrichment culture in the liquid medium within;
(4) carry out fermented and cultured in fermentation tank, the time of fermented and cultured is preferably greater than 70 hours, more preferably greater than 70.5 hours, as 72 hours; With
(5) purifying.
In step (1), the mutation of streptomyces noursei Xichang refers to the CGMCC No.0194 bacterial strain that is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center and is equal to bacterial strain, as having the bacterial strain of identical 16s rRNA with CGMCC No.0194 bacterial strain.These bacterial strains can obtain easily by buying on market.Rejuvenation preferably 26-30 ℃ (as, 28 ℃) and 65%-90% (as, 80%) the condition of relative moisture under carry out, usually on solid culture medium aerobic culture 14-18 (as, 16) day.In this article, solid culture medium contains corn flour, soybean cake powder, glucose, corn starch, dusty yeast, NaCl, CaCO
3, (NH
4)
2SO
4, KH
2PO
4, MgSO
4And agar powder, preferably its weight ratio prescription is: corn flour 3%, and soybean cake powder 4%, glucose 1%, corn starch 1%, dusty yeast 0.1%, NaCl 0.5%, CaCO
30.5%, (NH
4)
2SO
40.2%, KH
2PO
40.04%, MgSO
40.35%, agar powder 2.2%, surplus is a water, regulates pH value to 6.8 with NaOH.
In step (2), cultivate mycelium preferably 26-30 ℃ (as, 28 ℃) and 65%-90% (as, 80%) the condition of relative moisture under aerobic cultivate 12-16 (as, 14) sky.Get the mycelium of turning out and be used for enrichment culture thereafter.
In step (3), enrichment culture preferably 26-30 ℃ (as, 28 ℃) condition under cultivate (as, concussion is cultivated) 12-16 (and as, 14) day, more preferably in first liquid nutrient medium, cultivating.In this article, first liquid nutrient medium contains corn flour, soybean cake powder, glucose, corn starch, dusty yeast, NaCl, CaCO
3, (NH
4)
2SO
4, KH
2PO
4And MgSO
4, preferably its weight ratio prescription is: corn flour 3%, and soybean cake powder 4%, glucose 1%, corn starch 1%, dusty yeast 0.1%, NaCl 0.5%, CaCO
30.5%, (NH
4)
2SO
40.2%, KH
2PO
40.04%, MgSO
40.35%, surplus is a water, regulates pH value to 6.8 with NaOH.
In step (4), fermented and cultured preferably 26-30 ℃ (as, 28 ℃) and 6-10L/ hour (as, 8L/ hour) the condition of throughput under cultivate, more preferably in second liquid nutrient medium, cultivating.In this article, second liquid nutrient medium contains corn flour, soybean cake powder, glucose, corn starch, dusty yeast, NaCl, CaCO
3, (NH
4)
2SO
4, KH
2PO
4And MgSO
40.35%, preferably its weight ratio prescription is: corn flour 2%, and soybean cake powder 3%, glucose 1.5%, corn starch 1%, dusty yeast 0.2%, NaCl 0.5%, CaCO
30.5%, (NH
4)
2SO
40.2%, KH
2PO
40.04%, MgSO
40.35%, surplus is a water, regulates pH value to 6.8 with NaOH.
In step (5), purifying preferably includes acidifying, filtration and drying.The inventor finds, the purifying process (as, chromatography etc.) that composition of the present invention need not by complexity prepares, and only needs simple acidifying, filtration and dry step to get final product.Particularly, acidifying preferably add acid (as, oxalic acid) acidifying pH to 5-6 (as, 5.6); Filtration preferably include with tentatively filter the filtrate that (as, press filtration) obtain and be heated to 75-95 ℃ (as, 85 ℃), be cooled to then 0-10 ℃ (as, 4 ℃), then use membrane filtration; Drying is the negative-pressure vacuum drying preferably, and its baking temperature is no more than 60 ℃, and preferred 40-60 ℃, more preferably 45-55 ℃, as 50 ℃.
In second aspect, the invention provides pesticidal preparations, it comprises acceptable assistant on described composition pesticide of first aspect present invention and the agricultural chemicals.Pesticidal preparations is tangible product, can be missible oil (EC), pulvis (DP), granule (GR), wetting powder (WP), finish (OL), microemulsion (ME), suspending agent (SC), suspension emulsion (SE), water dispersible granules (WG) or aqueous emulsion preparations such as (EW).In this article, " acceptable assistant on the agricultural chemicals " refers to can add in agricultural chemicals and prepares the auxiliary material that forms preparation.Auxiliary material does not normally have the reagent of disease-resistant worm activity, comprises carrier or filler (as, water), wetting agent, dispersant, thickener, defoamer, emulsifier, antifreezing agent, cosolvent, disintegrant, etc.Typical auxiliary material comprises lauryl sodium sulfate, sodium lignin sulfonate, sodium metnylene bis-naphthalene sulfonate, kaolin, ethylene glycol, xanthans, silicone oil, water, dimethylbenzene, calcium dodecyl benzene sulfonate, phenethyl phenol polyethenoxy ether, triphen ethyl-phenol polyoxyethylene, Negel, ammonium sulfate and calcium carbonate, etc.In the pesticidal preparations of a second aspect of the present invention, preferred described auxiliary agent is selected from one or more of lauryl sodium sulfate, sodium lignin sulfonate, sodium metnylene bis-naphthalene sulfonate, kaolin, ethylene glycol, xanthans, silicone oil, water, dimethylbenzene, calcium dodecyl benzene sulfonate, phenethyl phenol polyethenoxy ether, triphen ethyl-phenol polyoxyethylene, Negel, ammonium sulfate and calcium carbonate.
The pesticidal preparations of second aspect present invention preferably is selected from the preparation of following prescription:
(1) composition and the weight portion thereof of fluorine bacterium azoles+Ningnanmycin wetting powder (WP) prescription: fluorine bacterium azoles 1-60; Ningnanmycin or contain the composition 1-40 of Ningnanmycin; Lauryl sodium sulfate 2; Sodium lignin sulfonate 4; Sodium metnylene bis-naphthalene sulfonate 5; Complement to 100 with kaolin;
(2) composition and the weight portion thereof of fluorine bacterium azoles+Ningnanmycin suspending agent (SC) prescription: fluorine bacterium azoles 1-60; Ningnanmycin or contain the composition 1-40 of Ningnanmycin; Sodium lignin sulfonate 3; Sodium metnylene bis-naphthalene sulfonate 2; Ethylene glycol 4; Xanthans 0.2; Silicone oil 0.3; Water complements to 100;
(3) composition and the weight portion thereof of fluorine bacterium azoles+Ningnanmycin microemulsion (ME) prescription: fluorine bacterium azoles 1-60; Ningnanmycin or contain the composition 1-40 of Ningnanmycin; Dimethylbenzene 10; Calcium dodecyl benzene sulfonate 5; Phenethyl phenol polyethenoxy ether 7; Triphen ethyl-phenol polyoxyethylene 4; Ethylene glycol 4; Water complements to 100;
(4) composition and the weight portion thereof of fluorine bacterium azoles+Ningnanmycin aqueous emulsion (EW) prescription: fluorine bacterium azoles 1-60; Ningnanmycin or contain the composition 1-40 of Ningnanmycin; Dimethylbenzene 10; Calcium dodecyl benzene sulfonate 5.5; Phenethyl phenol polyethenoxy ether 7.5; Triphen ethyl-phenol polyoxyethylene 4; Ethylene glycol 4; Water complements to 100;
(5) composition and the weight portion thereof of fluorine bacterium azoles+Ningnanmycin water dispersible granules (WG) prescription: fluorine bacterium azoles 1-60; Ningnanmycin or contain the composition 1-40 of Ningnanmycin; Negel 4; Sodium lignin sulfonate 8; Lauryl sodium sulfate 3; Ammonium sulfate 5; Calcium carbonate complements to 100.
Wherein, more preferably in Ningnanmycin or contain the composition of Ningnanmycin and the weight ratio of fluorine bacterium azoles is 1-40: 1-60, be preferably 1-10: 10-50, more preferably 2-6: 15-40, as 4: 25,6: 15,2: 30,2: 40 etc.
In the third aspect, the invention provides the application in the viroses of plant, mycosis and/or bacteriosis that control Ningnanmycin and fluorine bacterium azoles can be prevented and treated of described composition of first aspect present invention or the described pesticidal preparations of third aspect present invention.The present invention preferably provides described composition of first aspect present invention or the application of the described pesticidal preparations of third aspect present invention in control plant stripe virus disease and/or powdery mildew.
The present invention has following beneficial effect: can effectively prevent and treat the viroses of plant, mycosis, bacteriosis; Control efficiency has synergistic function, and can reduce the consumption of fluorine bacterium azoles, thereby reduces cost; Alleviate or suspension of pesticide resistance generation; Constant product quality; Fermentation preparation can disposablely be prepared the composition that contains Ningnanmycin in the composition of the present invention, etc.
For the ease of understanding, below will describe in detail the present invention by concrete accompanying drawing, embodiment.It needs to be noted that these descriptions only are exemplary descriptions, do not constitute limitation of the scope of the invention.According to the argumentation of this specification, many variations of the present invention, change all are conspicuous concerning one of ordinary skill in the art.
In addition, the present invention has quoted open source literature, and these documents are in order more clearly to describe the present invention, and their full text content is all included this paper in and carried out reference, just looks like that repeated description is the same excessively in this article for their full text.
Description of drawings
Fig. 1 is the mass spectral analysis collection of illustrative plates of formula 2 compounds, and wherein last figure is its mass-spectrogram, and figure below is the second order ms collection of illustrative plates of its fragment ion.
Fig. 2 is the mass spectral analysis collection of illustrative plates of formula 3 compounds, and wherein last figure is its mass-spectrogram, and figure below is the second order ms collection of illustrative plates of its fragment ion.
Embodiment
Further specify content of the present invention by the following examples.As do not specialize, conventional means that used technological means is well known to those skilled in the art among the embodiment and commercially available common instrument, reagent can be referring to the references such as manufacturers instruction of " microbiology experiment (the 4th edition) " (Higher Education Publishing House), " pesticidal preparations " (Chinese agriculture publishing house) and corresponding instrument and reagent.
Frozen streptomyces noursei Xichang mutation (available from Heilongjiang Province's biopesticide Engineering Technical Research Centre) is inoculated on the freshly prepared solid culture medium, in 28 ℃, 80% relative moisture, under the condition of filtrated air circulation, cultivate and carried out rejuvenation in 16 days, the bacterial classification spore that picking produces.Aerial mycelium then is about to the bacterial classification spore inoculating on freshly prepared solid culture medium, and in 28 ℃, 80% relative moisture, aerobic was cultivated 14 days under the condition of filtrated air circulation, high-visible have mycelium to produce.Carry out enrichment culture then, promptly mycelium washed from the solid culture basal plane and enter the 1L conical flask with freshly prepared first liquid nutrient medium, adding freshly prepared first liquid nutrient medium, to be supplemented to culture volume be 250ml, in 28 ℃, cultivated 14 days with the 250rpm shaking table.Through the thalline that microscope sampling detects each bottle cultivation have all have normal morphology after, carry out fermented and cultured, the culture fluid that promptly merges 4 conical flasks, inject 10L fermentation tank (can available from German Bei Lang company), with second liquid culture and be settled to 5L, in 28 ℃ of fermented and cultured 72 hours, condition of culture is: the blowing air amount was 8L/ hour, and mixing speed is 250rpm.
Wherein, the weight ratio of solid culture medium prescription is as follows: corn flour 3%, and soybean cake powder 4%, glucose 1%, corn starch 1%, dusty yeast 0.1%, NaCl 0.5%, CaCO
30.5%, (NH
4)
2SO
40.2%, KH
2PO
40.04%, MgSO
40.35%, agar powder 2.2%, surplus is a water, regulates pH value to 6.8 with NaOH; The weight ratio prescription of first liquid nutrient medium is as follows: corn flour 3%, and soybean cake powder 4%, glucose 1%, corn starch 1%, dusty yeast 0.1%, NaCl 0.5%, CaCO
30.5%, (NH
4)
2SO
40.2%, KH
2PO
40.04%, MgSO
40.35%, surplus is a water, regulates pH value to 6.8 with NaOH; The weight ratio prescription of second liquid nutrient medium is as follows: corn flour 2%, and soybean cake powder 3%, glucose 1.5%, corn starch 1%, dusty yeast 0.2%, NaCl 0.5%, CaCO
30.5%, (NH
4)
2SO
40.2%, KH
2PO
40.04%, MgSO
40.35%, surplus is a water, regulates pH value to 6.8 with NaOH.
Get the zymotic fluid after above-mentioned 72 hours, under condition of stirring, add oxalic acid at once and regulate pH to 5.6, inject BYJ650 template frame filter press (can available from Jin Hai Yongchang, Hangzhou filter press Co., Ltd) press filtration then.Collect filtrate and be heated to 85 ℃, keep moving into the cryogenic freezing case at once the temperature of filtrate being reduced to 4 ℃ after 2 minutes.Cross 0.45 micron filter membrane then, collect filtrate, place negative-pressure vacuum drying box (can available from Nanjing step butterfly heating equipment Co., Ltd) to be dried to solid in 50 ℃, promptly get the composition (abbreviating the Ningnanmycin that embodiment 1 prepares as) that the present invention contains Ningnanmycin with the pressure of 0.05MPa.
Prepared 5 batches of compositions that contain Ningnanmycin respectively according to above-mentioned method, carried out constituent analysis, wherein: the moisture content of each sets of batches compound is 0.35-0.39% all less than 0.5%; The stable content of Ningnanmycin in composition is 40.49-41.32% about 41%; The mass spectral analysis collection of illustrative plates of formula 2 compounds as shown in Figure 1, its stable content in composition is 41.28-46.52% about 44%; The mass spectral analysis collection of illustrative plates of the compound of formula 3 as shown in Figure 2, its stable content in composition is 12.03-17.07% about 15%.
The prescription of embodiment 2 Recompounded pesticides
1,25% fluorine bacterium azoles+4% Ningnanmycin wetting powder (WP): get fluorine bacterium azoles 25g; The Ningnanmycin 4g of embodiment 1 preparation; Lauryl sodium sulfate (wetting agent) 2g; Sodium lignin sulfonate (dispersant) 4g; Sodium metnylene bis-naphthalene sulfonate (NNO, dispersant) 5g; Kaolin (filler) complements to 100g, is ground into wetting powder.
2,25% fluorine bacterium azoles+4% Ningnanmycin suspending agent (SC): get fluorine bacterium azoles 25g; The Ningnanmycin 4g of embodiment 1 preparation; Sodium lignin sulfonate (dispersant) 3g; Sodium metnylene bis-naphthalene sulfonate (NNO, dispersant) 2g; Ethylene glycol (antifreezing agent) 4g; Xanthans (thickener) 0.2g; Silicone oil (defoamer) 0.3g; Deionized water complements to 100g.
3,15% fluorine bacterium azoles+6% Ningnanmycin microemulsion (ME): get fluorine bacterium azoles 15g; The Ningnanmycin 6g of embodiment 1 preparation; Dimethylbenzene (cosolvent) 10g; Calcium dodecyl benzene sulfonate (emulsifier) 5g; Phenethyl phenol polyethenoxy ether (emulsifier) 7g; Triphen ethyl-phenol polyoxyethylene (emulsifier) 4g; Ethylene glycol (antifreezing agent) 4g; Deionized water complements to 100g, is emulsified into homogeneous liquid, forms microemulsion.
4,30% fluorine bacterium azoles+2% Ningnanmycin aqueous emulsion (EW): get fluorine bacterium azoles 30g; The Ningnanmycin 2g of embodiment 1 preparation; Dimethylbenzene (cosolvent) 10g; Calcium dodecyl benzene sulfonate (emulsifier) 5.5g; Phenethyl phenol polyethenoxy ether (emulsifier) 7.5g; Triphen ethyl-phenol polyoxyethylene (emulsifier) 4g; Ethylene glycol (antifreezing agent) 4g; Deionized water complements to 100g, is emulsified into aqueous emulsion.
5,40% fluorine bacterium azoles+2% Ningnanmycin water dispersible granules (WG): get fluorine bacterium azoles 40g; The Ningnanmycin 2g of embodiment 1 preparation; Negel (dispersant) 4g; Sodium lignin sulfonate (dispersant) 8g; Lauryl sodium sulfate (wetting agent) 3g; Ammonium sulfate (disintegrant) 5g; Precipitated calcium carbonate (filler) complements to 100g, pulverizes.
The control efficiency of embodiment 3 Recompounded pesticides
We use according to commercially available 30% triflumizole wettable powder (available from Tso Tat Co., Ltd., Japan) of 25% fluorine bacterium azoles of embodiment 2 preparation+4% Ningnanmycin wetting powder (WP1) dilution back and equivalent (WP2) and the 25% fluorine bacterium azoles+4% Ningnanmycin wetting powder (WP3 for preparing with former medicine; It is with reference to the corresponding prescription of embodiment 2, but replaces with the former medicine of Ningnanmycin (available from Seoul Heilongjiang Qianger biochemical technology development Co., Ltd)) do field trial relatively, in order to show its control efficiency to each crops.
(1) field control effectiveness test of stripe disease
The high diseased region in Henan Province, with 2 mu of rice test field five equilibriums, use WP1, WP2, WP3 and blank (CP) respectively to test a area respectively, to wherein the plantation paddy rice in early July equivalent dispenser once, at interval after 7 days again the equivalent dispenser once after last medication, observed the effect of preventing and treating stripe disease on the 7th day.The result is shown in table 3-1, surprisingly, the control efficiency of WP1 and WP3 obviously is better than WP2, shows in the Recompounded pesticide that active ingredient such as Ningnanmycin can work in coordination with fluorine bacterium azoles and play synergistic effect, but also has reduced the consumption of more expensive fluorine bacterium azoles effectively; And the Ningnanmycin of embodiment 1 preparation and the former medicine of Ningnanmycin do not make significant difference to the synergistic effect of Recompounded pesticide, can exchange use.
The control efficiency table of table 3-1 Recompounded pesticide and 30% fluorine bacterium azoles
| Processed group | The rice strain number of sampling | Diseased plant rate (%) | Prevent and treat efficient (%) |
| WP1 | 732 | 6.69 | 69.6 |
| WP2 | 712 | 12.1 | 45.0 |
| WP3 | 658 | 6.38 | 71.0 |
| |
700 | 22.0 | - |
Observed in one month after the medication, each processed group does not have any bad reaction to paddy growth, does not observe the drug toxicity incident of injury yet.This shows that Recompounded pesticide can be promoted the use of safely.
(2) field control effectiveness test of wheat powdery mildew
The high diseased region in Henan Province with 2 mu of experimental field of wheat five equilibriums, uses WP1, WP2, WP3 and blank (CP) respectively to test a area respectively, and the wheat equivalent dispenser of wherein plantation was once observed the effect of preventing and treating wheat powdery mildew on the 30th day in the back.The result is respectively shown in table 3-2, surprisingly, the control efficiency of WP1 and WP3 slightly is better than WP2, shows in the Recompounded pesticide that active ingredient such as Ningnanmycin can work in coordination with fluorine bacterium azoles and play synergistic effect, but also has reduced the consumption of more expensive fluorine bacterium azoles effectively; And the Ningnanmycin of embodiment 1 preparation and the former medicine of Ningnanmycin do not make significant difference to the synergistic effect of Recompounded pesticide, can exchange use.
The control efficiency table of table 3-2 Recompounded pesticide and 30% fluorine bacterium azoles
| Processed group | The wheat strain number of sampling | Diseased plant rate (%) | Prevent and treat efficient (%) |
| WP1 | 317 | 1.9 | 74.0 |
| WP2 | 321 | 2.2 | 69.9 |
| WP3 | 357 | 2.0 | 72.6 |
| |
300 | 7.3 | - |
One and a half months is observed after the medication, and each processed group does not have any bad reaction to wheat growth, does not observe the drug toxicity incident of injury yet.This shows that Recompounded pesticide can be promoted the use of safely.
Claims (10)
1. composition pesticide, it comprises Ningnanmycin and fluorine bacterium azoles, wherein Ningnanmycin be purifying or provide by the composition that contains Ningnanmycin.
2. the described composition pesticide of claim 1, it is made up of composition that contains Ningnanmycin and fluorine bacterium azoles, and the wherein said composition that contains Ningnanmycin comprises Ningnanmycin, formula 2 compounds and formula 3 compounds as follows
3. claim 1 or 2 described composition pesticides, Ningnanmycin or contain the composition of Ningnanmycin and the weight ratio of fluorine bacterium azoles is 1-40: 1-60 wherein, be preferably 1-10: 10-50, more preferably 2-6: 15-40 most preferably is 4: 25.
4. the described composition pesticide of claim 2, the wherein said composition comprises water amount that contains Ningnanmycin is lower than 1% (w/w), preferably is lower than 0.5% (w/w), more preferably less than 0.4% (w/w).
5. the described composition pesticide of claim 2, the content of Ningnanmycin is preferably 40-50% (w/w) greater than 35% (w/w) in the wherein said composition that contains Ningnanmycin, and 40-45% (w/w) more preferably is as 40-42% (w/w).
6. the described composition pesticide of claim 2, the wherein said content that contains composition Chinese style 2 compounds of Ningnanmycin is 35-55% (w/w), is preferably 40-48% (w/w); And/or the content of formula 3 compounds is 7-23% (w/w), is preferably 11-18% (w/w).
7. the described composition pesticide of claim 2, the wherein said composition that contains Ningnanmycin are to prepare by the time that prolongs fermented and cultured, and more preferably it prepares by the following method:
(1) rejuvenation streptomyces noursei Xichang mutation, preferably rejuvenation under the condition of 28 ℃ and 80% relative moisture;
(2) cultivate mycelium on solid culture medium, preferably aerobic was cultivated 14 days under the condition of 28 ℃ and 80% relative moisture;
(3) carry out enrichment culture in the liquid medium within, preferably under 28 ℃ condition, cultivated 14 days;
(4) carry out fermented and cultured in fermentation tank, the time of preferred fermented and cultured is preferably greater than 70 hours, more preferably greater than 70.5 hours, as 72 hours, is more preferably under the condition of throughput of 28 ℃ and 8L/ hour and cultivates; With
(5) purifying preferably includes acidifying, filtration and drying.
8. pesticidal preparations, it comprises acceptable assistant on the arbitrary described composition pesticide of claim 1-7 and the agricultural chemicals, and preferred described auxiliary agent is selected from one or more of lauryl sodium sulfate, sodium lignin sulfonate, sodium metnylene bis-naphthalene sulfonate, kaolin, ethylene glycol, xanthans, silicone oil, water, dimethylbenzene, calcium dodecyl benzene sulfonate, phenethyl phenol polyethenoxy ether, triphen ethyl-phenol polyoxyethylene, Negel, ammonium sulfate and calcium carbonate.
9. the described pesticidal preparations of claim 8, it is missible oil (EC), pulvis (DP), granule (GR), wetting powder (WP), finish (OL), microemulsion (ME), suspending agent (SC), suspension emulsion (SE), water dispersible granules (WG) or aqueous emulsion (EW).
10. claim 8 or 9 described pesticidal preparations, it is the preparation that is selected from following prescription:
(1) composition and the weight portion thereof of fluorine bacterium azoles+Ningnanmycin wetting powder (WP) prescription: fluorine bacterium azoles 1-60; Ningnanmycin or contain the composition 1-40 of Ningnanmycin; Lauryl sodium sulfate 2; Sodium lignin sulfonate 4; Sodium metnylene bis-naphthalene sulfonate 5; Complement to 100 with kaolin;
(2) composition and the weight portion thereof of fluorine bacterium azoles+Ningnanmycin suspending agent (SC) prescription: fluorine bacterium azoles 1-60; Ningnanmycin or contain the composition 1-40 of Ningnanmycin; Sodium lignin sulfonate 3; Sodium metnylene bis-naphthalene sulfonate 2; Ethylene glycol 4; Xanthans 0.2; Silicone oil 0.3; Water complements to 100;
(3) composition and the weight portion thereof of fluorine bacterium azoles+Ningnanmycin microemulsion (ME) prescription: fluorine bacterium azoles 1-60; Ningnanmycin or contain the composition 1-40 of Ningnanmycin; Dimethylbenzene 10; Calcium dodecyl benzene sulfonate 5; Phenethyl phenol polyethenoxy ether 7; Triphen ethyl-phenol polyoxyethylene 4; Ethylene glycol 4; Water complements to 100;
(4) composition and the weight portion thereof of fluorine bacterium azoles+Ningnanmycin aqueous emulsion (EW) prescription: fluorine bacterium azoles 1-60; Ningnanmycin or contain the composition 1-40 of Ningnanmycin; Dimethylbenzene 10; Calcium dodecyl benzene sulfonate 5.5; Phenethyl phenol polyethenoxy ether 7.5; Triphen ethyl-phenol polyoxyethylene 4; Ethylene glycol 4; Water complements to 100;
(5) composition and the weight portion thereof of fluorine bacterium azoles+Ningnanmycin water dispersible granules (WG) prescription: fluorine bacterium azoles 1-60; Ningnanmycin or contain the composition 1-40 of Ningnanmycin; Negel 4; Sodium lignin sulfonate 8; Lauryl sodium sulfate 3; Ammonium sulfate 5; Calcium carbonate complements to 100.
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| CN 201110039684 CN102067840B (en) | 2011-02-17 | 2011-02-17 | Compound pesticide containing triflumizole and ningnanmycin |
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Cited By (6)
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| CN103371188A (en) * | 2012-04-15 | 2013-10-30 | 陕西韦尔奇作物保护有限公司 | Synergistic bactericidal composition containing agricultural antibiotic |
| CN103918658A (en) * | 2014-04-24 | 2014-07-16 | 深圳诺普信农化股份有限公司 | Compound bactericide composition containing cyazofamid |
| CN104054712A (en) * | 2014-05-28 | 2014-09-24 | 深圳诺普信农化股份有限公司 | Sterilization composition containing boscalid |
| CN104068023A (en) * | 2014-07-09 | 2014-10-01 | 深圳诺普信农化股份有限公司 | Agricultural sterilization composition |
| WO2015160619A1 (en) * | 2014-04-16 | 2015-10-22 | Bayer Cropscience Lp | Compositions comprising ningnanmycin and a fungicide |
| US9192166B2 (en) | 2013-02-11 | 2015-11-24 | Bayer Cropscience Lp | Compositions comprising a streptomyces-based biological control agent and an insecticide |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103371188A (en) * | 2012-04-15 | 2013-10-30 | 陕西韦尔奇作物保护有限公司 | Synergistic bactericidal composition containing agricultural antibiotic |
| CN103371188B (en) * | 2012-04-15 | 2017-03-29 | 长江大学 | A kind of Synergistic bactericidal composition containing antibiotic in pyrimidine nucleoside class |
| US9192166B2 (en) | 2013-02-11 | 2015-11-24 | Bayer Cropscience Lp | Compositions comprising a streptomyces-based biological control agent and an insecticide |
| WO2015160619A1 (en) * | 2014-04-16 | 2015-10-22 | Bayer Cropscience Lp | Compositions comprising ningnanmycin and a fungicide |
| CN103918658A (en) * | 2014-04-24 | 2014-07-16 | 深圳诺普信农化股份有限公司 | Compound bactericide composition containing cyazofamid |
| CN103918658B (en) * | 2014-04-24 | 2015-08-12 | 深圳诺普信农化股份有限公司 | A kind of combination sterilization composition containing cyazofamid |
| CN104054712A (en) * | 2014-05-28 | 2014-09-24 | 深圳诺普信农化股份有限公司 | Sterilization composition containing boscalid |
| CN104068023A (en) * | 2014-07-09 | 2014-10-01 | 深圳诺普信农化股份有限公司 | Agricultural sterilization composition |
| CN104068023B (en) * | 2014-07-09 | 2015-09-09 | 深圳诺普信农化股份有限公司 | A kind of agricultural bactericidal composition |
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| CN102067840B (en) | 2013-05-29 |
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