CN104041221A - Treatment method for seeds of notopterygium roots - Google Patents

Treatment method for seeds of notopterygium roots Download PDF

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Publication number
CN104041221A
CN104041221A CN201410308185.2A CN201410308185A CN104041221A CN 104041221 A CN104041221 A CN 104041221A CN 201410308185 A CN201410308185 A CN 201410308185A CN 104041221 A CN104041221 A CN 104041221A
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China
Prior art keywords
notopterygium root
notopterygium
seed
seeds
gibberellin
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Pending
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CN201410308185.2A
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Chinese (zh)
Inventor
陈志�
尚军
韩鸿萍
马永贵
乔枫
谢惠春
鲍敏
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Qinghai Normal University
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Qinghai Normal University
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Priority to CN201410308185.2A priority Critical patent/CN104041221A/en
Publication of CN104041221A publication Critical patent/CN104041221A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a treatment method for seeds of notopterygium roots and particularly relates to a method of germinating pretreatment. The method comprises the following steps: weighing 1000g of seeds of notopterygium roots; uniformly mixing the seeds of notopterygium roots by 50-500ppm auxin according to 20wt% of the seeds of notopterygium roots; treating for 90 days at 5-25 DEG C; uniformly mixing 50-500ppm (parts per million) gibberellin according to 25wt% of the seeds of notopterygium roots; treating for 60 days at 10-25 DEG C; counting 3 parts of the seeds of notopterygium roots after treatment, wherein each part contains 1000 seeds; germinating for 1 week at 20 DEG C and carrying out statistics on germination rate. With adoption of treatment by using auxin and gibberellin, the germination rate of the seeds of notopterygium roots can be remarkably improved to reach over 95%, thereby solving the key technical problem on manual standardized planting of notopterygium roots.

Description

A kind of notopterygium incisium seed processing method
Technical field
The invention belongs to seed treatment field, relate to a kind of notopterygium incisium seed processing method.
Background technology
Notopterygium root ( notopterygium incisum) be Umbelliferae Notopterygium medicinal plant; be precious Wild Medicinal Plants Resources, by < < China Precious, Rare, Endangered protective plant register > > and < < Chinese Wild herb resource protection management rules > >, divided and classify second class protection species and three grades of protection species in imminent danger as.
Notopterygium root tradition producing region is mainly the high and cold mountain area that Tibetan, the Qiang and other ethnic groups live in concentrated communities, mainly be distributed in the provinces such as Gansu, Qinghai, Sichuan, Tibet, habitat is special, owing to being difficult to domesticating and cultivating, crude drug source mainly relies on wild resource and excavates, the restriction of the reproductive characteristics such as seed development is incomplete owing to being subject to, latter stage of ripening is long, notopterygium root breeding and repopulate are mainly realized by vegetative propagation, and wild resource recovers to be subject to serious restriction.Notopterygium root has extensive application in traditional Chinese medicine and Tibetan medicine, under the driving of economic interests, transition is excavated, cause decline and the quantity of wild stocks to reduce, a large amount of forfeitures of notopterygium root and notopterygium forbesii germ plasm resource have been caused, in addition artificial breeding problem not yet solves, traditionally, be difficult to collect the propagating materialss such as sufficient seed and rhizome, the seriousness of notopterygium root degree in imminent danger can not be ignored.
Before not yet finding substituted plant and product at present, by wild plant introduction and acclimatization, carry out artificial cultivation, set up standardization production base, be to guarantee wild resource protection, the Sustainable Development and Utilization that realizes wild plant resource is one of the most effective approach.
Notopterygium root seed deep-sleep under nature, under natural endowment, one one summer of winter of seed demand could sprout.A large amount of seeds have been lost vitality between this very long resting stage, and germination rate is extremely low, and artificial cultivation plantation survival rate is low, and cost is large.Therefore,, for realizing the artificial standardized planting of notopterygium root, first must solve the low problem of notopterygium root seed germination rate.
Summary of the invention
The object of the embodiment of the present invention is to provide a kind of notopterygium incisium seed processing method, particularly sprouts the method for pre-treatment, is intended to solve the problem that artificial standardized planting notopterygium root seed germination rate is low, cost is large.
The embodiment of the present invention is achieved in that a kind of notopterygium incisium seed processing method, and the method comprises the following steps:
Take 1000 grams of notopterygium root seeds;
Adopt growth hormone to process notopterygium root seed;
Adopt gibberellin to process notopterygium root seed;
Number is got and is processed 3 parts, rear notopterygium root seed, 1000 every part, sprouts statistics percentage of seedgermination.
The method that further, adopts growth hormone to process notopterygium root seed is: adopt growth hormone to mix thoroughly by 20% of notopterygium root grain weight, place and process.
Further, the concentration of the growth hormone of processing notopterygium root seed is 50~500 ppm.
Further, the treatment temperature that adopts growth hormone to process notopterygium root seed is 5~25 ℃, the processing time is 90 days.
The method that further, adopts gibberellin to process notopterygium root seed is: adopt gibberellin to mix thoroughly by 25% of notopterygium root grain weight, place and process.
Further, the concentration of the gibberellin of processing notopterygium root seed is 50~500 ppm.
Further, the treatment temperature that adopts growth hormone to process notopterygium root seed is 10~25 ℃, the processing time is 60 days.
Further, number get and process after 3 parts, notopterygium root seed, 1000 every part, sprout 1 week statistics percentage of seedgermination for 20 ℃.
A kind of notopterygium incisium seed processing method of the present invention has following beneficial effect: under nature, during wild notopterygium root seed shedding, in proembryo state, germination rate is extremely low or do not sprout, and is difficult to realize artificial standardized planting.This technology can significantly be improved notopterygium root seed germination rate after adopting growth hormone to process, and it is reached more than 95%.For realizing the artificial standardized planting of notopterygium root, solved crucial technical problem.
Accompanying drawing explanation
Fig. 1 is the notopterygium root seed treatment process flow diagram that the embodiment of the present invention provides.
Fig. 2 is the growth hormone indole-3-acetic acid chemical structural drawing that the embodiment of the present invention provides.
Fig. 3 is the gibberellin A 3 chemical structural drawing that the embodiment of the present invention provides.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, a kind of notopterygium incisium seed processing method technical scheme of the present invention is further described.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
For convenience of explanation, only show part related to the present invention.
As shown in Figure 1-Figure 3, the invention provides a kind of notopterygium incisium seed processing method, the method comprises the following steps:
S101: take 1000 grams of seeds;
S102: adopt growth hormone to process notopterygium root seed;
S103: adopt gibberellin to process notopterygium root seed;
S104: number is got and processed 3 parts, rear notopterygium root seed, 1000 every part, sprouts;
S105: statistics percentage of seedgermination.
A prioritization scheme as the embodiment of the present invention: the method that adopts growth hormone to process notopterygium root seed is: adopt growth hormone to mix thoroughly by 20% of notopterygium root grain weight, place and process.
A prioritization scheme as the embodiment of the present invention: the concentration of processing the growth hormone of notopterygium root seed is 50~500 ppm.
A prioritization scheme as the embodiment of the present invention: the treatment temperature that adopts growth hormone to process notopterygium root seed is 5~25 ℃, and the processing time is 90 days.
A prioritization scheme as the embodiment of the present invention: the concentration of processing the gibberellin of notopterygium root seed is 50~500 ppm.
A prioritization scheme as the embodiment of the present invention: the temperature that adopts gibberellin to process notopterygium root seed is 10~25 ℃, and the processing time is 60 days.
A prioritization scheme as the embodiment of the present invention: number is got and processed 3 parts, rear notopterygium root seed,, sprouts 1 week statistics percentage of seedgermination for 20 ℃ by 1000 every part.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
Embodiment 1.
Taking 1000 seeds weighs.
Adopt concentration be 50~500 ppm growth hormone by 20% of notopterygium root grain weight, admix notopterygium root seed, mix thoroughly, in 5~25 ℃, place and process 90 days.
By 25% of notopterygium root grain weight, admixing concentration is 50~500 ppm gibberellin, mixes thoroughly, and in 10~25 ℃, number is got 1000 seeds and sprouted, and processes 60 days.
Number is got and is processed 3 parts, rear notopterygium root seed, 1000 every part, sprouts 1 week statistics percentage of seedgermination for 20 ℃.
Statistics percentage of seedgermination, notopterygium root Zhong Fa Ya Shuais≤and 95%.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.

Claims (4)

1. a notopterygium incisium seed processing method, is characterized in that the method comprises the following steps:
1) take 1000 grams of notopterygium root seeds;
2) adopt growth hormone to process notopterygium root seed: with concentration be 50~500 ppm growth hormone by 20% of notopterygium root grain weight, admix notopterygium root seed, mix thoroughly, in 5~25 ℃, place 90 days;
3) adopt gibberellin to process notopterygium root seed: with concentration be 50~500 ppm growth hormone by 20% of notopterygium root grain weight, admix notopterygium root seed, mix thoroughly, in 5~25 ℃, place 90 days;
4) number is got and is processed 3 parts, rear notopterygium root seed, 1000 every part, sprouts statistics percentage of seedgermination.
2. notopterygium incisium seed processing method as claimed in claim 1, it is characterized in that: the method that adopts gibberellin to process notopterygium root seed is: adopt gibberellin to mix thoroughly by 25% of notopterygium root grain weight, by 25% of notopterygium root grain weight, admix 50~500 ppm gibberellin, mix thoroughly, under 10~25 ℃ of conditions, place and process 60 days.
3. notopterygium incisium seed processing method as claimed in claim 1, it is characterized in that: the treatment temperature that adopts gibberellin to process notopterygium root seed is 10~25 ℃, by 25% of notopterygium root grain weight, admixes 50~500 ppm gibberellin, mix thoroughly, under 10~25 ℃ of conditions, place and process 60 days.
4. notopterygium incisium seed processing method as claimed in claim 1, is characterized in that: number is got and processed 3 parts, rear notopterygium root seed, 1000 every part, sprouts 1 week statistics germination rate for 20 ℃.
CN201410308185.2A 2014-07-01 2014-07-01 Treatment method for seeds of notopterygium roots Pending CN104041221A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106688358A (en) * 2015-07-23 2017-05-24 中国中医科学院中药研究所 Rapid germination of Chinese herb notopterygium forbesii seeds
CN108849499A (en) * 2018-05-28 2018-11-23 大连根特生物工程技术有限公司 A kind of expanding propagation method of the method for preparing Rhizoma Et Radix Notopterygii artificial seed and artificial seed
CN109041629A (en) * 2018-06-21 2018-12-21 四川千草生物技术股份有限公司 A kind of Rhizoma Et Radix Notopterygii seed afterripening processing method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5188655A (en) * 1988-01-21 1993-02-23 Jones Travis R Plant growth enhancing compositions using gibberellins, indoleacetic acid and kinetin
CN101133734A (en) * 2006-08-28 2008-03-05 青海师范大学 Cruentate fritillary seed processing method
CN101427680A (en) * 2007-11-10 2009-05-13 西宁集源生物科技有限公司 Notopterygium incisium seed processing method
CN103238399A (en) * 2013-04-28 2013-08-14 青海师范大学 Gentianopsis paludosa Ma. seed treatment method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5188655A (en) * 1988-01-21 1993-02-23 Jones Travis R Plant growth enhancing compositions using gibberellins, indoleacetic acid and kinetin
CN101133734A (en) * 2006-08-28 2008-03-05 青海师范大学 Cruentate fritillary seed processing method
CN101427680A (en) * 2007-11-10 2009-05-13 西宁集源生物科技有限公司 Notopterygium incisium seed processing method
CN103238399A (en) * 2013-04-28 2013-08-14 青海师范大学 Gentianopsis paludosa Ma. seed treatment method

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Title
张恩和 等: "野生羌活种子休眠机理及破除休眠技术研究", 《草地学报》 *
李宗仁 等: "羌活种子特性和发芽率研究(一)", 《青海大学学报(自然科学版)》 *
杨植松 等: "羌活种子贮藏和处理方法的研究", 《中草药》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106688358A (en) * 2015-07-23 2017-05-24 中国中医科学院中药研究所 Rapid germination of Chinese herb notopterygium forbesii seeds
CN108849499A (en) * 2018-05-28 2018-11-23 大连根特生物工程技术有限公司 A kind of expanding propagation method of the method for preparing Rhizoma Et Radix Notopterygii artificial seed and artificial seed
CN109041629A (en) * 2018-06-21 2018-12-21 四川千草生物技术股份有限公司 A kind of Rhizoma Et Radix Notopterygii seed afterripening processing method

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Application publication date: 20140917