CN103989727A - Acalypha australis L. and mequindox containing compound medicine for livestock and poultry - Google Patents

Abstract

The invention relates to an Acalypha australis L. and mequindox containing compound medicine for livestock and poultry. The compound medicine comprises the following raw materials in parts by weight: 3-30 parts of mequindox and 5-200 parts of Acalypha australis L. Studies prove that after Acalypha australis L. is added to an antibacterial medicine, the resistance of bacteria to the antibacterial medicine can be reversed, thus improving the treatment effects of the antibacterial medicine. By preparing the compound medicine into powder, tablets, oral liquid or granules according to conventional methods and adding the compound medicine to feeds for livestock and poultry according to the additive amount of 0.1-7g of main medicine/kg of feed to feed the livestock and poultry, the medicine cost and the medicine residues can be substantially reduced, higher economic benefits can be created for farmers, and food safety is simultaneously ensured.

Description

A kind of poultry compound medicine containing containing pearl grass and LIJUNJING

Technical field

The invention belongs to poultry escherichia coli and Salmonella infection treatment technology field, relate in particular to a kind of Guangxi precious jade medicine that contains containing the poultry compound medicine of pearl grass and LIJUNJING.

Background technology

Fowl salmonellosis (Avian salmonellosis) is the general name of the caused poultry disease of one or more Salmonellas in Salmonella.According to the difference of pathogen antigen structure, can be divided into 3 classes: Pullorum Disease, avian typhoid and avian paratyphoid.Wherein Pullorum Disease and avian typhoid Salmonella have host specificity, mainly cause chicken and turkey morbidity; Avian paratyphoid Salmonella can extensively infect many animals and people, and poultry that is subject to its pollution and products thereof is one of main source of mankind's Salmonella infection and alimentary toxicosis.The propagation circulation that the normal formation of fowl salmonellosis is quite complicated.Sick fowl and the fowl that carries disease germs are the main sources of infection, can pass through digestive tract, respiratory tract or eye conjunctiva horizontal transmission, after ovum vertical transmission, can infect poult, cause most of death, poultry carries disease germs for a long time, the ovum that then output is carried disease germs, if using, this hatches as hatching egg, that can go round and begin again is handed down from age to age, and causes fowl salmonellosis in chicken group, to be difficult to purify, and causes serious economic loss.Because Salmonella pollution face in chicken group is large, and easily produce drug resistance, immune effect of vaccine is in the market all undesirable.Therefore, breeder flock quarantine, medicine are controlled, environment disinfected purifies, strengthen feeding and management and health and epidemic prevention is still control and prevents that Salmonella from entering the essential measure of food chain.Chickling goes out to drink 0.01% potassium permanganate solution after shell or other drug carries out chemoprophylaxis for 1～2 day.This bacterium produces drug resistance to common drug, forces in production, to have to carry out various medicines and be used alternatingly, in the hope of better controlling fastbacteria.This bacterium produces stronger drug resistance to aminoglycoside medicaments at present, and aminoglycoside inactive enzyme is that the necessary group of some protection antibacterial activity in these antibacterials molecules is modified, and itself and the ribosomal affinity of target site are greatly reduced.These inactive enzymes comprise aminoglycoside acyltransferase (AAC), aminoglycoside adenylyl transferase (AAD), aminoglycoside phosphotransferase (APH), gland nucleotidyltransferase (ANT) etc.In the fastbacteria of many aminoglycosides, found at present the inactive enzyme of different qualities.

Escherichia coli are common conditioned pathogens, also be normal a kind of disease of sending out, its multiple location severe infections such as chicken acute sepsis, peritonitis, hepatitis, pneumonia, enteritis of can formerly having carried out the coffin upon burial, also can secondary or mixed sending out in virosis, to aquaculture, causes huge economic loss.The beta-lactam lopps antibiotic such as amoxicillin, ampicillin, ceftiofur, ceftriaxone, amoxicillin/clavulanate, ampicillin/sulbactam are the conventional important drugs of this disease of control, along with cultivation scale intensive degree is more and more large, medication is more and more spread unchecked, its Resistant strain is more and more many, also more about the report of Drug Resistance of E. coli.Studies confirm that, one of main mechanism of drug resistance is to produce beta-lactamase, especially extended spectrumβ-lactamase (Extended-Spectrum β-lactamase, ESBLs), this enzyme can be more by force and is hydrolyzed rapidly beta-lactam nucleus, makes this class antibiotic lose antibacterial activity.ESBLs is the main enzyme type of beta-lactamase, and it is not only to cephalo three generations and aztreonam drug resistance, and aminoglycoside, quinolones and sulfonamides are to crossing drug resistant, only to carbapenems, a mould alkene class medicaments insensitive.Many documents have reported colibacillary multi-drug resistant and have produced ESBLs and have substantial connection, both at home and abroad to comparatively pay close attention to.The detection, extraction, enzyme that inventor has carried out food animal source pathogenic bacterium ESBLs recently presses down the researchs such as enzyme protection effect to antibiotic percent hydrolysis and sulbactam, Tazobactam Sodium and Chinese medicine; to part ESBLs, colibacillary drug sensitive test shows, its multidrug resistant rate is apparently higher than non-ESBLs bacterial strain.In addition, result also shows to produce ESBLs bacterial strain not only to the serious drug resistance of the third generation cephalosporins such as ceftiofur, and also to multiple antimicrobial drug drug resistance such as fluoroquinolones, aminoglycoside and sulfonamides, fosfomycin etc., is serious multidrug resistant.Inventor is separated to the escherichia coli that tolerate P-levels mycin produces fosA3 drug resistant gene in producing ESBLs bacterial strain, has more strengthened the drug resistance of antibacterial.

Salmonella and colibacillary drug resistance cause raiser's drug cost to increase, and treatment extends the course for the treatment of, and mortality of livestock increases, and to production, brings larger economic loss, and increase poultry drug residue risk.Therefore, in actual production, need badly a kind of to no matter whether the Salmonella of drug resistance and the medicine that escherichia coli can have better therapeutical effect, and can shorten and treat the course for the treatment of, reduce mortality rate, reduce economic loss and reduce drug residue, for raiser creates higher economic benefit, also ensure that the mankind's is healthy and safe.

Summary of the invention

The technical problem to be solved in the present invention is to provide a kind of Guangxi precious jade medicine that contains containing the poultry compound medicine of pearl grass and LIJUNJING, it can kill antibacterial quickly, overcome antimicrobial drug treatment due to large, the residual height of weak curative effect, consumption and the high defect of cost that drug resistance problem causes, avoided that simple Chinese medicine sterilizing spectrum is narrow, slow problem sterilizes.

For solving above technical problem, the present invention by the following technical solutions: a kind of poultry compound medicine containing containing pearl grass and LIJUNJING, is comprised of the raw material of following weight portion: LIJUNJING 3-30 part, contains the careless 5-200 part of pearl.

Described compound medicine is prepared into powder, tablet, oral liquid or granule.

The preparation method of described compound medicine is: will be containing broken mistake 60 mesh sieve of pearl grass meal, then mix with pulverous antimicrobial drug, be prepared into according to a conventional method powder, tablet, oral liquid or granule.

Containing pearl grass (Acalypha australis L.), have another name called Acalypha australis, Herba Acalyphae, people Herba Amaranthi tricoloris, belong to Euphorbiaceae Acalypha, draft is to suffruticose draft, and stem is very thin, thick below 3 millimeters, female flower bract is lobate, is lobate greatly, and length can reach 1 centimetre, do not divide, there is sawtooth at edge, and base portion is heart-shaped, female flower is kidney shape when open, when closed, is clam, wherein keeps fruit, therefore have the reputation of " Acalypha australis ", also therefore and easily identification.Originate in various places, Guangxi, be born on the ground waste or more moistening place, limit, village.Herb decocts that water is for oral administration can treat bacillary dysentery, diarrhoea etc.

For the drug resistance problem of current poultry escherichia coli and Salmonella infection existence, inventor utilizes Guangxi precious jade medicine to form compound medicine containing pearl grass and antimicrobial drug.Studies have shown that, in antimicrobial drug, add containing after pearl grass, can reverse the drug resistance of bacteria medicine, thereby improve the therapeutic effect of antimicrobial drug.According to a conventional method this compound medicine is made to powder, tablet, oral liquid or granule, according to the addition of 0.1-7g principal agent/kg feedstuff, add in animal and fowl fodder and feed, can significantly reduce drug cost and drug residue, for raiser creates higher economic benefit, also guaranteed food safety simultaneously.

The specific embodiment

One, the drug resistance inversion effect research to antimicrobial drug containing pearl grass

Antimicrobial drug itself has the effect for the treatment of escherichia coli and Salmonella, but has stronger drug resistance owing to producing escherichia coli and the Salmonella of drug resistant gene, causes antimicrobial drug not show good therapeutic effect in normal using dosage situation.

1, containing pearl grass, mix with antimicrobial drug after to colibacillary drug resistance inhibition test

On 96 orifice plates, adopt microtest tube doubling dilution, first measured antimicrobial drug to the minimal inhibitory concentration of the e. coli strains of product ESBLs and fosA3 drug resistant gene and contained pearl grass to producing the subinhibitory concentration of ESBLs and fosA3 drug resistant gene e. coli strains, what then in culture medium, add subinhibitory concentration reuses containing pearl grass the minimal inhibitory concentration that microtest tube doubling dilution is cultivated mensuration antimicrobial drug; Utilize continuous passage method be added with same subinhibitory concentration containing the culture medium of pearl grass and same concentration antimicrobial drug in to producing the cultivation of going down to posterity of ESBLs and fosA3 drug resistant gene e. coli strains, until the antimicrobial drug minimal inhibitory concentration observing with go down to posterity before compare and diminish more than 4 times, can be judged as containing pearl grass antibacterial medical instrument is had to drug resistance inversion effect.Result of the test statistics is as shown in table 1:

Table 1 subinhibitory concentration (0.5g/mL) goes down to posterity and causes the variation (μ g/mL) of producing ESBLs and fosA3 escherichia coli Western medicine MIC containing pearl grass.

As seen from the above table, antimicrobial drug all reduces more than 4 times the colibacillary minimal inhibitory concentration of drug resistance, can judge containing pearl grass and have drug resistance inversion effect to producing ESBLs and fosA3 drug resistant gene escherichia coli.

2, containing pearl grass, mix with antimicrobial drug after drug resistance inhibition test to Salmonella

On 96 orifice plates, adopt microtest tube doubling dilution, first measured antimicrobial drug to the minimal inhibitory concentration of drug resistance salmonella strain with containing the subinhibitory concentration of pearl grass to drug resistance salmonella strain, what then in culture medium, add subinhibitory concentration reuses containing pearl grass the minimal inhibitory concentration that microtest tube doubling dilution is cultivated mensuration antimicrobial drug; Utilize continuous passage method be added with same subinhibitory concentration containing the culture medium of pearl grass and same concentration antimicrobial drug in to the cultivation of going down to posterity of Salmonella persister, until the antimicrobial drug minimal inhibitory concentration observing with go down to posterity before compare and diminish more than 4 times, can be judged as containing pearl grass antibacterial medical instrument is had to drug resistance inversion effect.Result of the test statistics is as shown in table 2:

Table 2 subinhibitory concentration (0.5g/mL) goes down to posterity and causes the variation (μ g/mL) of drug resistance Salmonella Western medicine MIC containing pearl grass.

As seen from the above table, antimicrobial drug all reduces more than 4 times the minimal inhibitory concentration of drug resistance Salmonella, can judge containing pearl grass drug resistance Salmonella is had to drug resistance inversion effect.

Two, the application example of compound medicine of the present invention

According to table 3 formula and following methods Preparation Example 1-embodiment 4: will be containing after broken mistake 60 mesh sieves of pearl grass meal, as basic material, then with pulverous antimicrobial drug by weight ratio put into blender and mix, compound medicine is prepared into formulation products.

The formula of table 3 embodiment 1-4 and preparation dosage form

Numbering	Antimicrobial drug	Containing pearl grass	Dosage form
				Embodiment 1	LIJUNJING 11g	5g	Oral liquid
Embodiment 2	LIJUNJING 20g	10g	Granule
				Embodiment 3	LIJUNJING 3g	45g	Powder
Embodiment 4	LIJUNJING 30g	180g	Tablet

1, the efficacy experiment of the compound medicine product of embodiment 1-4 gained to the piglet diarrhea of 33 ages in days

Clinical symptoms: Guangxi Fangcheng Port pig farm, 33 age in days piglets, this swinery after wean is turn-taked, starts to occur diarrhoea, draws watery stools, feces contains indigested feedstuff, pig material feeding but be exactly not grow, indivedual serious diarrhea of pigs dehydrations are dead, do not occur symptoms of emesis, raiser uses multi-medicament, and ideal effect does not all appear in treatment.

Cut open inspection pathological changes: after cuing open inspection, Intestinum Sus domestica wall is poor, especially small intestinal rear end and colon position, intestinal mucosa comes off, and intestinal wall is congested, hemorrhage.

Laboratory diagnosis: get pathologic liver and dye sheet, Gram’s staining, micro-Microscopic observation, the gram negative bacilli of red color visible; Tentatively be judged as bacillus coli.By escherichia coli, cultivate, biochemical test discriminating, judgement Infection in Piglets is bacillus coli, pcr amplification proves and produces ESBLs drug resistant gene and the fosA3 drug resistant gene that has CTX-M type.

Test therapeutic regimen: morbidity swinery is divided 6 groups of administrations, use embodiment 1 according to the addition administration of 0.3g/kg feedstuff for first group, use embodiment 2 according to the addition administration of 0.3g/kg feedstuff for second group, use embodiment 3 according to the addition administration of 3g/kg feedstuff for the 3rd group, use embodiment 4 according to the addition administration of 1.4g/kg feedstuff for the 4th group; Use LIJUNJING powder according to the addition administration of 0.2g/kg feedstuff for the 5th group, the 6th group with containing the thick powder of pearl grass according to the addition administration of 4g/kg feedstuff; Above six groups of therapeutic regimens all continue to use 5 days.

Result of use feedback: first group to the 4th group medication is after 2 days, and swinery is no longer drawn watery stools, feces is shaped, and no longer contains feedstuff in feces, continues to use 3 days swinery recovery from illness; The 5th group of medication is after 2 days, and swinery is still unhealthy, continue to use 3 days, substantially get well, but still draw pasty state feces individually, but drug withdrawal is after 3 days, and recurrence appears in swinery; The 6th group of medication is after 2 days, and swinery, without remarkable improvement, continues to use 3 days, and swinery continues to have loose bowels, and all the other are also more serious individually.Visible, contain containing the compound of pearl grass swinery curative effect is better than to folk prescription LIJUNJING and folk prescription containing pearl grass.

2, the efficacy experiment of embodiment 1-4 gained compound medicine product to Salmonella gallinarum disease.

Clinical symptoms: Nanning chicken house, 14 Day-old Broiler Chickens, there is dysentery in chicken group, row white, pasty state loose stool, perianal tomentum fecal pollution, has Calx sample lump, and what have sends painful shriek, has the phenomena of mortality.Cut open the indivedual Hepar Gallus domesticus enlargements of inspection and be kermesinus to darkviolet, or khaki slightly, matter is crisp easily broken, and surface is dispersed in or greyish white, the downright bad point of lark that gathers, and be red petechia sometimes, typically Salmonella genius morbi.

Laboratory diagnosis: get pathologic liver and dye sheet, Gram’s staining, micro-Microscopic observation, the gram negative bacilli of red color visible; Tentatively be judged as Salmonella disease.By Salmonella, cultivate, biochemical test is differentiated, judges that chicken infects for Salmonella disease, and drug sensitive test result is judged as persister Salmonella according to NCCLS standard.

Test therapeutic regimen: morbidity chicken group is divided into 6 groups of administrations, use embodiment 1 according to the addition administration of 0.3g/kg feedstuff for first group, use embodiment 2 according to the addition administration of 0.3g/kg feedstuff for second group, use embodiment 3 according to the addition administration of 3g/kg feedstuff for the 3rd group, use embodiment 4 according to the addition administration of 1.4g/kg feedstuff for the 4th group; Use LIJUNJING powder according to the addition administration of 0.2g/kg feedstuff for the 5th group, the 6th group with containing the thick powder of pearl grass according to the addition administration of 4g/kg feedstuff; Above six groups of therapeutic regimens all continue to use 5 days.

Effect feedback: after the chicken group medication of first group to the 4th group, sick chicken feed intake rises, and no longer occurs dead chicken, continues to use 3 days, and chicken group be almost recovered; The chicken group medication of the 5th group is after 2 days, and chicken group majority is still unhealthy, still has indivedual chicken deaths, continues to use 3 days, and chicken death phenomenon is not still effectively controlled; The chicken group medication of the 6th group is after 2 days, and chicken group does not have too large remarkable improvement, continues to use 3 days, and chicken death phenomenon is not still effectively controlled.Visible, the compound medicine curative effect containing containing pearl grass is better than folk prescription.

Claims (2)

1. contain the poultry compound medicine containing pearl grass and LIJUNJING, it is characterized in that, by the raw material of following weight portion, formed: LIJUNJING 3-30 part, contains the careless 5-200 part of pearl.

2. the poultry compound medicine containing containing pearl grass and LIJUNJING according to claim 1, is characterized in that, described compound medicine is prepared into powder, tablet, oral liquid or granule.